[Histonet] Humanized mouse monoclonals

[Thomas Crowell]

Dear Histonetters,

I was wondering if anyone could provide me with a basic protocol using a 
preformed complex of a humanized mouse monoclonal (human Fc ) with a 
biotinylated anti-human IgG to detect antigens in human cryostat sections 
- essentially, human on human IHC staining?  I have several antibodies 
with different total protein concentrations.

Thanks

Tom Crowell
BiogenIDEC
Cambridge, MA