FW: [Histonet] Azur B

[Brett, Lawrence]

Kristipher
Yes we use Azure B regularly as a counterstain for S100 or other
immunocytochemistry for melanoma.
Use a simple 1% aqueous solution of Azure B for 5 minutes after the immuno,
it turns the melanin green, making a nice contrast to the brown immunostain.
We don't usually counterstain it, you may have to stain with haematoxylin
first.

Lawrence Brett
Royal Infirmary
Edinburgh
Scotland, UK

-----Original Message-----
From: Kristopher Kalleberg [mailto:Kristopher.Kalleberg <@t> unilever.com]
Sent: 11 January 2005 13:39
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Azur B


HAs anyone used Azur(e) B for a melanin stain.  I read the other day that
you
can use Azur B to stain malanin a green-blue, but everything else I read
only
says it is used for plant tissue.  If anyone has used it for melanin, how
does
it work and what would be the best counterstain.  Thanks.

Kris L Kalleberg
Unilever R&D
45 River Rd. 
Edgewater, NJ 07020
201 840 2472


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