[Histonet] reversible tissue adhesion to slides

[Philip Oshel]

Janis,

How about attaching the part you *don't* want? That way the 
dissection will also release the desired tissue. Use a minute (no! 
smaller! still smaller ... ) drop of superglue to hold the tissue to 
the slide -- the same glue used to glue fresh tissue to the mounting 
plate of a vibrating microtome.

Phil

>Dear colleagues,
>
>We are looking for a method to very firmly attach unfixed larval
>Drosophila ventral ganglia to a glass slide so we can slice off one
>surface of the ganglion (to remove neurons that we don't want; it's a
>long story; e-mail me if you want to know), to be followed by
>*releasing the attached tissue* from the slide and preparing primary
>neuronal cultures.  The tissue needs to be kept alive and happy
>under saline.  Polylysine doesn't hold tight enough for the slicing
>step (which we do with a fine blade).  Possibilities that have come
>to mind are using more adhesive slides (e.g., coated with
>aminosilane?), using some sort of double-stick tape, or a
>releasable glue (e.g., something like vetbond but that can be made
>to release?).  Obviously part of the difficulty is to release the tissue
>without destroying it.   Does anyone have a suggestion?  thanks!
>
>Janis Weeks
>
>--=[|]=-- --=[|]=-- --=[|]=-- --=[|]=-- --=[|]=--
>Janis C. Weeks, Professor of Biology
>weeks <@t> uoneuro.uoregon.edu
>http://www.neuro.uoregon.edu/faculty/weeks.html
>Institute of Neuroscience
>1254 University of Oregon
>Eugene, OR  97403-1254
>voice (541)346-4517, FAX (541)346-4548
>--=[|]=-- --=[|]=-- --=[|]=-- --=[|]=-- --=[|]=--
>
>
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-- 
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison,  WI  53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)