[Histonet] apoptosis question

[Patsy Ruegg]

I have been doing cleaved caspase 3 from Cell Signaling with great success
on rat samples but one of my customers wants to use it on sheep cells and we
are not getting any staining.  Has anyone else gotten apoptosis signals from
sheep cells?

Patsy

The dilemma:

1. i have never seen caspase 3 (cleaved or other) by IHC in the sheep cells.

2. im not sure if they have it or the IHC ab doesnt detect it. - So i cant
draw a conclusion - 3. with dapi the nuclei look generally okay, as well as
by tunel stain on whole mount lungs

 

 

4 - what can i do without repeating expt on existing slides to confirm or
deny presence of apoptosis (on these sheep cells)???

Annexin V , tunel. apotag?