[Histonet] murine eosinophil immunostaining

[Gayle Callis]

We are embarking on murine eosinophil staining on FFPE fixed lung (not 
short fixation time!).  I will be doing other searches for available 
antibodies to detect eosinophils, but would like any recommendations from 
laboratories successfully doing murine eosinophil IHC.

1.  Whether this will work on FFPE tissue, if so, retrieval?

2.  Are frozen sections a preference - something I would prefer to do anyway.

3. Antibody source

Any other suggestions will be welcome as this is NOT a project that 
originated in our laboratory so we have no control on fixation time in NBF.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)