[Histonet] Warthin-Starry on B-5 fixed tissue

[Moklebust, Amanda C]

	I have been asked to try out a Warthin-Starry protocol previously
posted on the histonet (Slides are placed in 1% heated sliver nitrate, then
developed in a solution of silver nitrate, hydroquinone, and gelatin).  I am
only getting the control to stain if I skip our usual pretreatment for
mercury pigment.  No, the control was not fixed in B-5, but we normally put
everything into alcoholic iodine for 4 minutes, water rinse, then 1 minute
in sodium thiosulfate, and a final water rinse. I have not been working with
B-5 fixed tissue for very long, so I would like to know what other techs can
recommend.  Do I just continue to avoid alcoholic iodine?  Is there a better
option for B-5 fixed tissue?  Thanks in advance for your assistance. 

Amanda Moklebust,
Seattle Cancer Care Alliance
 


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