[Histonet] (no subject)

[Gayle Callis]

Depending on the kind of staining wanted, I have stained paraffin sections 
on slides that were stored for years and didn't have any adverse 
effects.  I was not doing immunostaining, just special stains.

Caveat:  some special stains may be affected by long term storage, one 
reported is Masson's Trichchrome for collagen.  I would also be careful 
about longer term storage for immunohistochemical stains.  Days are 
probably not a problem.

This has been discussed before on Histonet, check the archives for further 
info.

  and At 02:57 PM 1/3/2005, you wrote:
>Hello everybody,
>I was wondering how long paraplast sections that have been
>mounted on slides could be left alone after drying before
>staining.  Is there any reason they couldn't be left for days
>or weeks?
>Thanks for your advice,
>Frank
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)