[Histonet] C4d

[Drew Sally A.]

We are using C4d from Biogenesis(Cat.#2222-8004) at a 1:600 dilution.  We pretreat the slides using an EDTA buffer and HIER in BioCare Medical's DeCloaker. Our protocol on the Ventana immunostainer includes an amplification step. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of ANN
MARUSKA
Sent: Wednesday, January 05, 2005 9:02 AM
To: histonet <@t> pathology.swmed.EDU
Subject: [Histonet] C4d


Hi histonetters,
 
Is anyone using C4d on paraffin tissue for their transplant specimens
as an IHC stain?  I am interested in which product you are using and how
it has performed for you.
Thanks in advance.
 
Ann
 
Ann Maruska
Fairview-University Medical Center
Mpls. MN  55454
amarusk1 <@t> fairview.org
612-273-9119

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