[Histonet] The "Stuff" to Find Lymph Nodes

[clifford berger]

Hello Wanda,

Please take a look at Dissect Aid on our website, www.decal-bone.com.  It is 
a special fixative for revealing lymph nodes which we have been 
manufacturing for over 20 years. You can call our toll free number or reply 
to this email for a free sample.

Cliff Berger
Decal Chemical Corp
1800-428-5856
www.decal-bone.com







----- Original Message ----- 
From: "Patrick Paulusse" <wolf <@t> webhart.net>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>; "Smith Wanda" 
<Wanda.Smith <@t> HCAhealthcare.com>
Sent: Tuesday, January 04, 2005 6:58 PM
Subject: Re: [Histonet] The "Stuff" to Find Lymph Nodes


> Wanda,
>
> Although I have not been fortunate enough to have tried some of the
> commercially available lymph node retrieval solutions, I do use a homemade
> solution which was outlined in an article (referenced below) that you 
> might
> be interested in trying.  The recipe is as follows:
>
>    500 ml of absolute ethanol
>    170 ml distilled water
>    80 ml of 37% formaldehyde
>    50 ml of acetic acid
>
> According to the article the reagents must be added in this order (does
> anyone know why?).  I've used this since 2001 with great success.  I 
> dissect
> off the pericolic adipose tissue and leave it sit in the solution for 24
> hours and the smallest of lymph nodes show up white against the 
> grey/yellow
> adipose tissue. I've also let it sit over the weekend and have seen no
> deleterious affects because of the prolonged treatment.  If you try it let
> me know what you think.
>
> Article Reference:
>
> "GEWF solution: an inexpensive, simple, and effectiveaid for the retrieval
> of lymph nodes from colorectal cancer specimens". Ken J. Newell, Barry W.
> Sawka, Brian F. Rudick, David K. Driman. Arch Pathol Lab Medicine, May
> 2001;125:642-645.
>
> Patrick Paulusse
> Histology Supervisor
> Pembroke Regional Hospital
> Ontario, Canada
>
> ----- Original Message -----
> From: "Smith Wanda" <Wanda.Smith <@t> HCAhealthcare.com>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Tuesday, January 04, 2005 3:40 PM
> Subject: [Histonet] The "Stuff" to Find Lymph Nodes
>
>
>> Dear Histonetters,
>> My mind has gone blank...and I need help please!
>> What are some of the commercial solutions available to help ID lymph 
>> nodes
>> at the grossing table?  What are your experiences, pro and con, of these
>> solutions???
>> One of my Pathologist has read a few articles concerning finding lymph
> nodes
>> on colon cancer cases.  A high number of lymph nodes must be found, down
> to
>> the 1-2 mm size nodes and we are interested in other's experiences.
>> Thanks for any and all info!!!
>> Wanda
>>
>> > Wanda G. Smith,  HTL/HT(ASCP)
>> > Pathology Supervisor
>> > Trident Medical Center Laboratory Services
>> > *9330 Medical Plaza Drive, Charleston, SC 29406
>> > *843-797-4586 *fax 843-797-4296
>> > *wanda.smith <@t> hcahealthcare.com
>> >
>> >
>> >
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet