[Histonet] The "Stuff" to Find Lymph Nodes

[Favara, Cynthia (NIH/NIAID)]

Wanda, 

This is similar to Davidson Fixative that we use here for eyes.

I think the order is important for two reasons but would not want to be
tested on either.

Impurities are added to absolute ETOH and depending on what is added for
this purpose a precipitate may form when it is added to acidic solutions.
Formaldehyde will do the same. This is based on my very old knowledge and
many years of experience. In my experience the precipitate forms in the
solution interface and does not always redissolve.

I am confident someone will have a more scientific explanation and I look
forward to reading.

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Patrick Paulusse [mailto:wolf <@t> webhart.net] 
Sent: Tuesday, January 04, 2005 4:59 PM
To: Histonet; Smith Wanda
Subject: Re: [Histonet] The "Stuff" to Find Lymph Nodes

Wanda,

Although I have not been fortunate enough to have tried some of the
commercially available lymph node retrieval solutions, I do use a homemade
solution which was outlined in an article (referenced below) that you might
be interested in trying.  The recipe is as follows:

    500 ml of absolute ethanol
    170 ml distilled water
    80 ml of 37% formaldehyde
    50 ml of acetic acid

According to the article the reagents must be added in this order (does
anyone know why?).  I've used this since 2001 with great success.  I dissect
off the pericolic adipose tissue and leave it sit in the solution for 24
hours and the smallest of lymph nodes show up white against the grey/yellow
adipose tissue. I've also let it sit over the weekend and have seen no
deleterious affects because of the prolonged treatment.  If you try it let
me know what you think.

Article Reference:

"GEWF solution: an inexpensive, simple, and effectiveaid for the retrieval
of lymph nodes from colorectal cancer specimens". Ken J. Newell, Barry W.
Sawka, Brian F. Rudick, David K. Driman. Arch Pathol Lab Medicine, May
2001;125:642-645.

Patrick Paulusse
Histology Supervisor
Pembroke Regional Hospital
Ontario, Canada

----- Original Message -----
From: "Smith Wanda" <Wanda.Smith <@t> HCAhealthcare.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, January 04, 2005 3:40 PM
Subject: [Histonet] The "Stuff" to Find Lymph Nodes


> Dear Histonetters,
> My mind has gone blank...and I need help please!
> What are some of the commercial solutions available to help ID lymph 
> nodes at the grossing table?  What are your experiences, pro and con, 
> of these solutions???
> One of my Pathologist has read a few articles concerning finding lymph
nodes
> on colon cancer cases.  A high number of lymph nodes must be found, 
> down
to
> the 1-2 mm size nodes and we are interested in other's experiences.
> Thanks for any and all info!!!
> Wanda
>
> > Wanda G. Smith,  HTL/HT(ASCP)
> > Pathology Supervisor
> > Trident Medical Center Laboratory Services *9330 Medical Plaza 
> > Drive, Charleston, SC 29406
> > *843-797-4586 *fax 843-797-4296
> > *wanda.smith <@t> hcahealthcare.com
> >
> >
> >
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


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