[Histonet] RE:Quantum dot IHC

[Melissa Gonzalez]

Jakub,
I would suggest diluting further in ranges until you still see the
unspecific background disappear but retain the "real" staining. Also,
ofcourse, make sure the secondary is compatible with the primary
(ie-cross-reactivity between species). 
I am actually curious, you are the first person I have communicated with
that has given Quantum dots a try. What source are you using to
visualize the staining? How do you differentiate the different
wavelengths using these products? I am so happy with Alexa Fluors that I
haven't yet given the Quantum dots a go. 
Good luck, 

Happy New Year to everyone, 
Melissa




Message: 9
Date: Fri, 30 Dec 2005 13:28:07 +0100
From: Jakub Ot?hal <jotahal <@t> ftvs.cuni.cz>
Subject: [Histonet] Quantum dot immunohistochemistry
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002b01c60d3c$7d6110f0$8428e793 <@t> kubanotes>
Content-Type: text/plain;	charset="iso-8859-2"

Dear Histonet users,

I would like to ask you for advice. I am starting with quantum dot
conjugated secondary antibody immunohistochemistry. I have tried one
trial
with the same protocol I use with Alexa Fluor. Secondary antibody
concentration 1:100 (1:50 is recommended by chemicon), but I have
terrible
background. Does anybody have idea what is "best" working dilution?

 

Thank you very much

Happy New Year

Jakub Otahal

 

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-- 
 Jakub Otahal MD,PhD
Department of Developemental Epileptology 
Institute of Physiology 
Czech Academy of Sciences 
Videnska 1083 
142 20 Prague 4 
Czech Republic 
tel: +420 241062495

 <mailto:jotahal <@t> ftvs.cuni.cz> jotahal <@t> ftvs.cuni.cz
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