[Histonet] Re: Brain sections-rat

[kotresh mattur]

 Dear all,
            We are facing some problems in rat brain sectioning and staining. We are sectioning brain at 5-6 microns thickness and then we take sections at 37-40 centigrade after leaving for 1min. in water bath or till the sections are flattened. During staining at hydration step sections are lifting off. For this problem we tried to take the sections on histogrip coated slides which also did not help us at all. Whether there are any special delicate techniques in brain sectioning, staining? Regarding this problem can anybody help with their vast experience? Suggestions/advice from all are welcome.

 Thanks in advance

Dr.K.Y.Mathur



Dr.K.Y.Mathur
Junior Scientist
Dept.of Preclincal Safety Evaluation
Discovery Research
Dr.Reddy's Laboratory Ltd.
Bollaram Road, Miyapur,
HYDERABAD-INDIA-49.
 
Ph No: 
040-23045439-exn 424,421,422,423
Cell :09391293129

		
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