Hello, We are doing stereotaxic surgeries on rats in my lab. After 2 weeks, we fixate the brain via perfusion using 4% PFA (after vascular rinse) and then immerse the brains in 4% PFA overnight. My question is where can I find information on pump flow rate and PFA volume to use during fixation? Thanks. Wisam Barkho daufoi <@t> msn.com