[Histonet] RE: Frozen sections
C.M. van der Loos
c.m.vanderloos <@t> amc.uva.nl
Mon Apr 4 09:46:20 CDT 2005
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<TBODY> <TR> <TD colSpan=2> <P><FONT face=Times
size=2>Hi,</FONT></P> <P><FONT face=Times
size=2>Good question! </P> <P><FONT face=Times
size=2>Actually we never tried to fix&n acetone before storage at -80C. </FONT>The only t know is that proper storage at -80C of unfixed cryostat section works well for a long time. Storing them unfixed gives you at least
t example, antigens preferabl much better in this res face=Times size=2>Chris van der Loos, PhD<BR>D Pathology<BR>Academical Medical Center M2-230<BR>Meiber gdreef 9<BR>NL-1105 AZ Amsterdam<BR>The
Netherlands</FONT>< size=2>phone: +31 20 5665631< BR>fax: +31 20
6960389<BR>e-mail: href="mailto:c.m.vanderloos <@t> amc.uva.nl" face=Times size=2>c.m.vanderloos <@t> amc.uva.nl< /FONT></A></P> <P><FONT face=Times
size=2></FONT> </P> <P><FONT face=Times
size=2>----- Original Message -----</FO NT></P></TD></TR> <TR> <TD
align=right><B><FONT face=Times size=2>From </FONT></B></TD> <TD><FONT face=Times
size=2>Tyler <mtyler <@t> uctgsh1. uct.ac.za></FONT></TD></TR> <TR> <TD
align=right><B><FONT face=Times size=2>Date </FONT></B></TD> <TD><FONT face=Times
size=2>Mon, 04 Apr 2005 12:06:16 + 0200</FONT></TD></TR> <TR> <TD
align=right><B><FONT face=Times size=2>To </FONT></B></TD> <TD><FONT face=Times
size=2>histonet <histonet <@t> lists .utsouthwestern.edu></FONT></TD></TR> <TR>
<TD align=right><B><FONT face=Times
size=2>Subject&nb <TD><FONT face=Times size=2>[Histonet] Frozen
sections< <P><FONT face=Times size=2>Hi<BR>Asking this question
for sections. if<BR>one fixes cry slides at -80°C?<BR>Is i sections unfixed before freezing? Thank< BR>you<BR>Marilyn<BR></FONT></P></BODY>
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