From juan.gutierrez <@t> christushealth.org Fri Apr 1 05:42:05 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:52 2005 Subject: [Histonet] Glass Coverslippers Message-ID: We have the Leica CV5030, I'm not sure if it is the one you are referring to, and are finally contempt with it. We were probably one of the first ones to buy it and had to work some bugs out. The coverslipper sat on a counter unused for over six months waiting for a hardware and software upgrade. After it was upgraded we had constant problems with it. The problems ranged from: incorrect coverslip placement on the slide to actual slide breakage(nothing makes me happier than having to recut slides). But in all fairness, and after repeated visits from the service techs, we have a good workhorse now. The service has improved tremendously with the new Field Service Engineer(Steven Work). If you decide to buy it make sure the installer doesn't leave until you are 100% satisfied with the instruments operation. The new one out of the box might not work as good as the demo unit. Another unit you might want to consider is the Meisei Robot Coverslipper. I'm not sure who's selling it now, but we use to have one that was very hard to beat. Old age and careless techs finally did it in. It works on the same principle as the Leica, but they've had longer to work out the bugs. Like the disclaimer at the bottom says, this is MY personal opinion and not the one of my employers and their lawyers. Good luck on your search. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grant, Debra Sent: Thursday, March 31, 2005 2:11 PM To: Histonet Subject: [Histonet] Glass Coverslippers Hi All, We would like to purchase a glass coverslipper very soon, and are interested in positive and negative feedback on all glass coverslippers. Thanks for your time in advance! P.S Is anyone demoing the Leica Coverslipper at the moment? Debby Grant Histology Specialist I Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org (913)926-4305 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Fri Apr 1 05:50:24 2005 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:24:52 2005 Subject: FW: [Histonet] Glass Coverslippers Message-ID: I tried both the Leica and the Sakura. I chose the Sakura, it simply fitted my needs better. The Leica has more bells and whistles that I did not need, there were fewer moving parts, if there was a "hang up" of the slides/coverslips for some reason the Sakura allowed easier access to retrieve them, easier to clean. For me, it was just easier to use overall. Of course glass is definitely a slower process but we are not clinical and are not under such strict time constraints. Just my two cents. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacy McLaughlin Sent: Thursday, March 31, 2005 2:23 PM To: 'Grant, Debra'; Histonet Subject: RE: [Histonet] Glass Coverslippers Debby, We will be demoing the Leica Coverslipper next week. I'll be glad to let you know how it goes. Does anyone have any experience with the Sakura Glas coverslipper? We're trying that one in a few weeks. Thanks. Stacy McLaughlin -----Original Message----- From: Grant, Debra [mailto:DRG@Stowers-Institute.org] Sent: Thursday, March 31, 2005 3:11 PM To: Histonet Subject: [Histonet] Glass Coverslippers Hi All, We would like to purchase a glass coverslipper very soon, and are interested in positive and negative feedback on all glass coverslippers. Thanks for your time in advance! P.S Is anyone demoing the Leica Coverslipper at the moment? Debby Grant Histology Specialist I Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org (913)926-4305 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If the reader of this e-mail message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this e-mail message in error, please immediately notify Cooley Dickinson Healthcare at 413-582-2000 and delete or shred the original message and all copies thereof. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Fri Apr 1 08:00:44 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] ASCP BOR - answers to questions In-Reply-To: <00b201c5365a$6a3d83a0$50d7d445@domainnotset.invalid> Message-ID: Peggy, 2005 is the last year ASCP will provide Tech Sample for Histology. When I inquired why, I was told that not enough people were purchasing the Histology Tech Sample. This is a sad note because of the requirement to have newly registered Histo techs to have 30 hours of CE every threes. The requirement for CEUs goes up, but the routes to obtain the CEU goes down. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of lpwenk@sbcglobal.net Sent: Thursday, March 31, 2005 7:31 PM To: Histonet Subject: [Histonet] ASCP BOR - answers to questions Going to try to answer several questions in this one email. If not interested in ASCP BOR or in some answers - please push the delete button now. 1. WHO TO TALK TO ABOUT COMPLAINTS ABOUT ASCP BOARD OF REGISTRY (BOR) The NSH appointed representative to ASCP BOR is Marilyn Gamble. There is a link to her email at the NSH web site http://www.nsh.org - click on Who We Are - click on Appointments - click on Marilyn's email link under ASCP BOR The list of histotechs and pathologists on the ASCP BOR Histotechnology exam committee can be found on the ASCP BOR webpage. There are no email links to their names. http://www.ascp.org/bor/about - click on Histotechnology Examination Committee Also, you can contact ASCP BOR directly via phone or email. You could ask them a question, or ask for the phone number on the Histotechnology exam committee. The list of staff people working at the BOR and their roles can be found on: http://www.ascp.org/bor/about/ - at the bottom of the page, click on Contact an ASCP Board of Registry staff member. 2. WHY THE INCREASE IN THE COST OF GRADING HT/HTL PRACTICAL EXAMS? Well, the ASCP BOR staff member was at NSH S/C in Toronto, and talked about it at the booth, and at several committee meetings. I also called ASCP BOR in the fall for additional information, which they gave out. Nothing hidden or secret about it. Ask, and they will tell. The HT and HTL exams are the most expensive technician/technologist exams for ASCP BOR, because HT/HTL/pathologists graders have to be brought in from all other the USA, two weekends a year, to grade all the sets. The fee paid by the HT/HTL candidates did not cover the cost of the ASCP grading the written (computer) and the practical HT/HTL exams. ASCP BOR had made several changes recently, to keep the cost down. Instead of submitting 15 slides, BOR statistically found that 9 slides were sufficient to achieve the same pass/fail rate as 15 slides. (In other words, if someone does a good job on cutting and staining 9, they would have done the same good job on 15. And the same with someone doing a not so good/bad job.) By reducing the number of slides, they reduced the number of people needed to grade the slides, thereby reducing costs. A new change is that the candidate must pass the written/computer portion first, before they can submit their practical. This will reduce the number of practicals being sent in. This again will reduce the number of graders needed, which will keep costs down. However, unfortunately, these changes could not keep the costs down enough, and now ASCP BOR is asking the candidates to help to cover SOME of the costs of grading the practical exams, by paying an additional $75. Notice the word SOME. This additional fee still does not cover all the costs. 3. WHAT DO I, AS A HISTOTECH, GET BY BEING AN ASCP MEMBER? Maybe not as much as being an NSH member, but I still think I get some things. - Tech Samples (for continuing education) - ASCP Teleconferences (at least 3 each quarter are histology. Others on management are also helpful) - "Laboratory Medicine" (which might not have as much histology articles as I might like, but it gives me a better understanding of how all the labs fit in together. Helpful in management issues.) - Representatives from ASCP in Washington DC, working on: laws for payment and reimbursement which effect my pay; getting grants to start new lab schools; etc. - Books like the Frieda Carson textbook, HT/HTL exam study books, Jules Elias book on IHC - Annual wage and salary survey And all of these things cost money to set up and run. Money from ASCP members. All of the above information I obtained by A) calling ASCP BOR and asking them questions I have, B) attending committee meetings at NSH where the ASCP BOR staff are there to answer questions, and C) talking with the ASCP BOR staff that are at the ASCP BOR booth in the exhibit hall that the NSH S/C, D) reading the ASCP BOR web page, "Laboratory Medicine", and anything else I can get. It's not a secret. And calling does help. Sometimes it takes a while, but things do change. I've called the NSH representatives to ASCP BOR and the chair of the ASCP BOR Histotechnology exam committee in the past with concerns, ideas, suggestions, questions. I've gotten answers, and often they have incorporated my suggestions into changing things on the exams (different tissues, stains, checking on a question when my students swear there wasn't a right answer (typo, it turned out, which the BOR QC had already caught so the students' scores were not effected, etc.)). I've raised questions about the wage and salary survey, and that was modified. I've offered suggestions about their flyers about histotechs, and these were changed in the next revision (about 4 years later - slowly, remember?). No, they didn't change these things because it was ME who called. They changed because I offered a good suggestion. There are also things they did not change. So it goes. No, it isn't perfect. But from where I stand, I think they need more constructive input and more people being involved. That's us, people. We care about our field, otherwise we wouldn't still be in the field and on Histonet. So let's be constructive and offer concrete suggestions and ideas for improvement to our representatives - email, call, and attend the NSH Education Committee or Instructors in Histotechnology meeting in Fort Lauderdale. It takes more than 6 people sitting in on a committee, to make changes. It's sad when the meetings are open to everyone of the 1200 people in attendance, and only 6 people show up. (That is my personal opinion, not something gleaned from a book or webpage.) Off my soap box for a while. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Fri Apr 1 08:04:19 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] PTAH Mordant In-Reply-To: <006b01c53651$7d68d3c0$50d7d445@domainnotset.invalid> Message-ID: Ann Preece, now that's going back to the good ole days. Thank you all for your answers. The majority are using Bouin's as a mordant and staining the usual way. That's why I love you all. Even an old goat (cabrito here in Texas) like me keeps learning from y'all. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of lpwenk@sbcglobal.net Sent: Thursday, March 31, 2005 6:27 PM To: Joe Nocito; Histonet Subject: Re: [Histonet] PTAH Mordant We use Bouin fixative - 60 degree oven for 1 hour (after deparaffinizing slides and running them down to water). (Can skip the Lugolizing steps) Then wash in running water until yellow disappears from the tissue (1-10 minutes, depending on type of tissue. The more striated muscle there is, the longer it takes.) Then stain as usual in PTAH. We get better, more consistent staining with this method. One of my fellow students found it when we were in training. We had to do a PTAH for our practical. Worked great. I believe it was from the Ann Preece book. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Joe Nocito" To: "Histonet" Sent: Thursday, March 31, 2005 2:27 PM Subject: [Histonet] PTAH Mordant > Hey y'all, > since we no longer have Zenkers because of the mercury, what is being used > as a mordant for the PTAH stain? My pathologists have been hounding me all > week, but with my other net postings and replies, I just forgot. Thanks > Histoland > > > Joe Nocito, BS, HT(ASCP) QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Fri Apr 1 08:42:46 2005 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Glass Coverslippers Message-ID: <63B8B599DE283148B92E83C78B32C15D8B4DC2@cmhexbe2.childrensmemorial.org> Hi all, We are looking for LCM- Laser capture microscope. Is anybody has experience with that? We are thinking between Leica and Arcturus. Any response is appreciated. Naira -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GUTIERREZ, JUAN Sent: Friday, April 01, 2005 5:42 AM To: Grant, Debra; Histonet Subject: RE: [Histonet] Glass Coverslippers We have the Leica CV5030, I'm not sure if it is the one you are referring to, and are finally contempt with it. We were probably one of the first ones to buy it and had to work some bugs out. The coverslipper sat on a counter unused for over six months waiting for a hardware and software upgrade. After it was upgraded we had constant problems with it. The problems ranged from: incorrect coverslip placement on the slide to actual slide breakage(nothing makes me happier than having to recut slides). But in all fairness, and after repeated visits from the service techs, we have a good workhorse now. The service has improved tremendously with the new Field Service Engineer(Steven Work). If you decide to buy it make sure the installer doesn't leave until you are 100% satisfied with the instruments operation. The new one out of the box might not work as good as the demo unit. Another unit you might want to consider is the Meisei Robot Coverslipper. I'm not sure who's selling it now, but we use to have one that was very hard to beat. Old age and careless techs finally did it in. It works on the same principle as the Leica, but they've had longer to work out the bugs. Like the disclaimer at the bottom says, this is MY personal opinion and not the one of my employers and their lawyers. Good luck on your search. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grant, Debra Sent: Thursday, March 31, 2005 2:11 PM To: Histonet Subject: [Histonet] Glass Coverslippers Hi All, We would like to purchase a glass coverslipper very soon, and are interested in positive and negative feedback on all glass coverslippers. Thanks for your time in advance! P.S Is anyone demoing the Leica Coverslipper at the moment? Debby Grant Histology Specialist I Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org (913)926-4305 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. From RBARNHART <@t> summithealth.org Fri Apr 1 08:53:53 2005 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] National Histology Walkout Message-ID: I have only worked in on lab in my 10 yrs (I know it is pretty unheard of). And I have always been appreciated by the pathologist in what ever role I have been in. And for pay I really can't complain we are in the middle to high according to the ASCP survey. I think there should be something done about the ASCP in regards to histology education. I have a horrible time trying to find any. Sometimes I wonder why I keep renewing my membership. >>> Larry Woody 3/31/2005 4:06:41 PM >>> I too have been lucky enough to work at some places that treated me with respect, ie; the Mayo Clinic, and my current job at Amgen, but the field is under paid and a lot of places you don't get respect or treated fairly. Someone delivering packages for UPS makes more than the average Tech, is that right. Larry --------------------------------- Do you Yahoo!? Yahoo! Mail - You care about security. So do we. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DRG <@t> Stowers-Institute.org Fri Apr 1 09:14:10 2005 From: DRG <@t> Stowers-Institute.org (Grant, Debra) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Glass coverslippers Message-ID: <1099754255-747195340@pathology.swmed.edu> Hi All, Thanks for all your great responses. We will be trying the Leica coverslipper next week! :-) have a great weekend! Debby Grant Histology Specialist I Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org From relia1 <@t> earthlink.net Fri Apr 1 09:22:51 2005 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Relia Job Alert!! Florida Licensed Histotechnologist needed Message-ID: Hi Histonetters, I am presently on a search for one of my best clients located in Southeastern Florida. My client is in need of a Florida licensed histotechnologist. They offer excellent compensation, benefits and relocation assistance. This is a full time 40 hour per week M-F day shift position. If you or anyone you know might be interested in hearing more about this opportunity please call me toll free at 866-607-3542 or e-mail me at relia1@earthlink.net Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net From gcallis <@t> montana.edu Fri Apr 1 10:18:26 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: Immunofluorescence and background In-Reply-To: References: <8362520.1112176318135.JavaMail.jerry.lyons@ucd.ie> Message-ID: <6.0.0.22.1.20050401091533.01b45210@gemini.msu.montana.edu> PFA increase autofluorescence in tissues. You could use a different color fluorophore if you are doing immunofluorescent staining. I am attaching a review of autofluorescence to you privately, although it is related to GFP it is still a very fine review of what you are experiencing. You may not be able to reduce autofluorescence, although some have used borohydride treatments with some success, and various other means, but it is a problem. At 05:08 PM 3/31/2005, you wrote: >I have a different but similar problem with Immunofluorescence and >background. I'm cutting rat spinal cord sections that have been perfused and >post fixed in 4% paraformaldehyde, cryoprotected and cut as frozen sections. > > >The autofluorescence in the tissue is so high I am unable to clearly see >fluorescent labelled cells. > > >Is this a problem of the fixation in 4% paraformaldehyde? or a property of >spinal cord? or is there something obvious I should or shouldn't be doing? > > >Regards, cath > >---------------------------------------------------------------------------- > >Catherine Gorrie >School of Medical Sciences >University of New South Wales >Sydney, NSW > >ph: 02 9385 2462 >fax: 02 9385 8016 >e-mail: c.gorrie@unsw.edu.au >---------------------------------------------------------------------------- > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From dellav <@t> musc.edu Fri Apr 1 04:58:52 2005 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] ASCP BOR - answers to questions Message-ID: As always Peggy, you are informative, helpful and the voice of reason. Thank you. all very good suggestions. My worst fear would be that a "walkout" would do little but draw negative attention to a discipline little known by the public. this simply isn't the kind of publicity that would serve to advance the profession or correct the conditions lamented by some on this list. the views expressed above are my own and are not intended to represent the views of the NSH Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> 03/31/05 20:31 PM >>> Going to try to answer several questions in this one email. If not interested in ASCP BOR or in some answers - please push the delete button now. 1. WHO TO TALK TO ABOUT COMPLAINTS ABOUT ASCP BOARD OF REGISTRY (BOR) The NSH appointed representative to ASCP BOR is Marilyn Gamble. There is a link to her email at the NSH web site http://www.nsh.org - click on Who We Are - click on Appointments - click on Marilyn's email link under ASCP BOR The list of histotechs and pathologists on the ASCP BOR Histotechnology exam committee can be found on the ASCP BOR webpage. There are no email links to their names. http://www.ascp.org/bor/about - click on Histotechnology Examination Committee Also, you can contact ASCP BOR directly via phone or email. You could ask them a question, or ask for the phone number on the Histotechnology exam committee. The list of staff people working at the BOR and their roles can be found on: http://www.ascp.org/bor/about/ - at the bottom of the page, click on Contact an ASCP Board of Registry staff member. 2. WHY THE INCREASE IN THE COST OF GRADING HT/HTL PRACTICAL EXAMS? Well, the ASCP BOR staff member was at NSH S/C in Toronto, and talked about it at the booth, and at several committee meetings. I also called ASCP BOR in the fall for additional information, which they gave out. Nothing hidden or secret about it. Ask, and they will tell. The HT and HTL exams are the most expensive technician/technologist exams for ASCP BOR, because HT/HTL/pathologists graders have to be brought in from all other the USA, two weekends a year, to grade all the sets. The fee paid by the HT/HTL candidates did not cover the cost of the ASCP grading the written (computer) and the practical HT/HTL exams. ASCP BOR had made several changes recently, to keep the cost down. Instead of submitting 15 slides, BOR statistically found that 9 slides were sufficient to achieve the same pass/fail rate as 15 slides. (In other words, if someone does a good job on cutting and staining 9, they would have done the same good job on 15. And the same with someone doing a not so good/bad job.) By reducing the number of slides, they reduced the number of people needed to grade the slides, thereby reducing costs. A new change is that the candidate must pass the written/computer portion first, before they can submit their practical. This will reduce the number of practicals being sent in. This again will reduce the number of graders needed, which will keep costs down. However, unfortunately, these changes could not keep the costs down enough, and now ASCP BOR is asking the candidates to help to cover SOME of the costs of grading the practical exams, by paying an additional $75. Notice the word SOME. This additional fee still does not cover all the costs. 3. WHAT DO I, AS A HISTOTECH, GET BY BEING AN ASCP MEMBER? Maybe not as much as being an NSH member, but I still think I get some things. - Tech Samples (for continuing education) - ASCP Teleconferences (at least 3 each quarter are histology. Others on management are also helpful) - "Laboratory Medicine" (which might not have as much histology articles as I might like, but it gives me a better understanding of how all the labs fit in together. Helpful in management issues.) - Representatives from ASCP in Washington DC, working on: laws for payment and reimbursement which effect my pay; getting grants to start new lab schools; etc. - Books like the Frieda Carson textbook, HT/HTL exam study books, Jules Elias book on IHC - Annual wage and salary survey And all of these things cost money to set up and run. Money from ASCP members. All of the above information I obtained by A) calling ASCP BOR and asking them questions I have, B) attending committee meetings at NSH where the ASCP BOR staff are there to answer questions, and C) talking with the ASCP BOR staff that are at the ASCP BOR booth in the exhibit hall that the NSH S/C, D) reading the ASCP BOR web page, "Laboratory Medicine", and anything else I can get. It's not a secret. And calling does help. Sometimes it takes a while, but things do change. I've called the NSH representatives to ASCP BOR and the chair of the ASCP BOR Histotechnology exam committee in the past with concerns, ideas, suggestions, questions. I've gotten answers, and often they have incorporated my suggestions into changing things on the exams (different tissues, stains, checking on a question when my students swear there wasn't a right answer (typo, it turned out, which the BOR QC had already caught so the students' scores were not effected, etc.)). I've raised questions about the wage and salary survey, and that was modified. I've offered suggestions about their flyers about histotechs, and these were changed in the next revision (about 4 years later - slowly, remember?). No, they didn't change these things because it was ME who called. They changed because I offered a good suggestion. There are also things they did not change. So it goes. No, it isn't perfect. But from where I stand, I think they need more constructive input and more people being involved. That's us, people. We care about our field, otherwise we wouldn't still be in the field and on Histonet. So let's be constructive and offer concrete suggestions and ideas for improvement to our representatives - email, call, and attend the NSH Education Committee or Instructors in Histotechnology meeting in Fort Lauderdale. It takes more than 6 people sitting in on a committee, to make changes. It's sad when the meetings are open to everyone of the 1200 people in attendance, and only 6 people show up. (That is my personal opinion, not something gleaned from a book or webpage.) Off my soap box for a while. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Fri Apr 1 10:36:25 2005 From: cforster <@t> umn.edu (Colleen Forster) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Walk Out Message-ID: <424D7889.3070906@umn.edu> I agree with Vinnie. We want to draw positive attention not negative as we are already the "unknown". Colleen Forster U of Mn From slappycraw <@t> yahoo.com Fri Apr 1 10:44:19 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] ASCP BOR - answers to questions Message-ID: <20050401164419.92672.qmail@web52608.mail.yahoo.com> Key words there "little known by the general public", is exactly why places get away with hiring unqualified people in labs. I don't know where most of the people who respond on this list are working but if you haven't worked in a lab with unqualified people then in my opinion you've been very lucky. When a lab hires someone who doesn't have the degree or the BOR and they are considered the same level it is demeaning and it's just wrong and what has the ASCP done about it. This profession is in the closet and it's high time someone brought it out to the public. Larry --------------------------------- Do you Yahoo!? Yahoo! Mail - 250MB free storage. Do more. Manage less. From gcallis <@t> montana.edu Fri Apr 1 11:02:49 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Tech Sample discontinuation Message-ID: <6.0.0.22.1.20050401095050.01b9f290@gemini.msu.montana.edu> Sadly, a supply and demand consideration. If people won't buy it, then it isn't going to be produced. Having written a Tech Sample, it takes an unbelievable amount of time to produce it. The effort by authors (who are asked to do this) and the road to final sample (editing, etc ) was as much work as a juried publication in a major journal. There are and will be many other ways to keep histotechs' continuing education current - and I foresee on-line as the wave of the future, faster and easier to produce. One way or the other, we will stay well informed - if required, so be it. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From STEGTM <@t> samcstl.org Fri Apr 1 11:02:19 2005 From: STEGTM <@t> samcstl.org (Therersa Stegall) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] ASCP BOR - answers to questions Message-ID: You are so right. Especially if that uncertified employee has seniority. Some days I bounce around the lab like a rubber monkey, between ascessioning, cutting, staining, IHC and specials, frozens (and all of the troubleshooting and equipment maintenance); while the "microtomy monkeys" sit with their arms folded after the mornings' run has been sectioned and stained. And they make more $$$ than I do. I have 2 degrees, have been certified for more years than I want to talk about, and...... The thing is, I love what I do, and try to learn more always. Maybe I wouldn't be so frustrated if I just chucked it, and went to manage a Quik Trip - those folks get more for slinging gas, beer/coffee, cigs than we licensed medical professionals do. Okay, I feel better now. Peace, Terre From Barry.R.Rittman <@t> uth.tmc.edu Fri Apr 1 11:30:40 2005 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Walk Out Message-ID: <566FB0B522443D43AF02D2ADBE35A6F00163F365@UTHEVS3.mail.uthouston.edu> A walkout does nothing. I believe that like most organizations, he ASCP has its good and its bad points. It does provide some very useful learning aids and in all fairness is a powerful and organized advocate for the pathology profession. This is important in the current climate when insurance companies in Texas are trying to squirm out of paying for laboratory tests. On the other hand the organization is one that provides the tests for histotechs who will be employed by pathologists. This is, for a general principal an unhealthy relationship. The employers are doing the certification for those who will be future employees in one form or another. What incentive is there for such an organization to push for higher salaries for histotechs? What is obviously required is an independent testing and certification agency. This would make for a much more open marketplace for histotechs. This however is not the only problem. When I trained in England salaries were pitiful. You have to remember this was a long time ago (I think that Noah had just landed the ark). My salary was around the equivalent of a cowboy in the 1850s. Then a union became organized for the nurses and the MLTs joined. Within 3 to 4 years salaries had tripled. While I know that Texas is a right to work state and also that unions have their bad as well as their good points, they can act as powerful lobbies. Who precisely is lobbying specifically for us now? How many of you have written to the ASCP and to legislators voicing your concerns for our profession and stressing what would happen if we did not have dedicated but underpaid people working in our laboratories? We all tend to be somewhat isolated, individually, statewide, and as a nation. If you work for a really good pathologist who is concerned for your welfare and does their best for pay increases for you, it is difficult to worry about other individuals who are not as fortunate in their workplaces. As long as the histology profession consists of individual small groups and certification is controlled by potential employers I do not think that anything will change. I feel that ASCP and other groups need to be convinced that we are all part of a team. All members of that team must be totally committed to the team's mission of providing great health care. All members of the team should also share in the financial and other benefits that come from such efforts. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Colleen Forster Sent: Friday, April 01, 2005 10:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Walk Out I agree with Vinnie. We want to draw positive attention not negative as we are already the "unknown". Colleen Forster U of Mn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gervaip <@t> aol.com Fri Apr 1 11:38:32 2005 From: Gervaip <@t> aol.com (Gervaip@aol.com) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] ASCP BOR - answers to questions- this is going to hurt! Message-ID: <46.66818b59.2f7ee118@aol.com> Vinnie, it is true, we are already the unknown. And much of it has to do with our stubbornness to accept the fact that if we want to be included with MTs and CTs, and fly with the eagles, we need to do our part when it comes to education. Personally, I could not just walk out and would not be able to do it. But we have an NSH representative on the ASCP Board. Why can Marilyn Gamble not bring this to their attention and see what steps can be taken to correct an old situation. I for one, think that we should have gone for it.... the 4 year degree and just bit the bullet. The associate degree is only a small step forward. Is it going to take another 20 years to make it a degreed registry? Pearl Gervais, BS, HT/HTL And I might add, I did not complete my degree until after I got my HTL. If I can do it, others can too. I worked 50 hour weeks, had two young children and commuted 65 miles to work. And I still attended workshops, local, out of state and the national to learn more before it was required here in Louisiana to have CEUs. Not easy, but it can be done. From slappycraw <@t> yahoo.com Fri Apr 1 11:50:33 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] ASCP BOR - answers to questions- this is going to hurt! Message-ID: <20050401175033.58883.qmail@web52610.mail.yahoo.com> It takes extreme positions to change the status quo because starting with an extreme position you end up where you want to be. When you ask for a salary you don't start on the low end do you? Larry __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gliuygao <@t> hotmail.com Fri Apr 1 13:05:24 2005 From: gliuygao <@t> hotmail.com (yan gao) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] test In-Reply-To: <20050401175033.58883.qmail@web52610.mail.yahoo.com> Message-ID: test From Janet.Bonner <@t> FLHOSP.ORG Fri Apr 1 13:08:14 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] National Histology Walkout Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4207@fh2k093.fhmis.net> There was a message yesterday, but I honestly believe it was in jest. Unless you live in Chicago, IL, there isn't anywhere to "walk" out of!! - -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: WWmn916@aol.com; histonet@lists.utsouthwestern.edu Sent: 3/31/2005 4:44 PM Subject: Re: [Histonet] National Histology Walkout Deborah, Did I miss something? Who said they were going to walk out? From what I have read they are going to boycott not renewing their ASCP stickers. Did I miss a message? Robyn >>> 03/31/05 12:56 PM >>> Wow, I must be living in a bubble in the Sacramento area. I personally don't feel victimized or taken advantage of in this profession. Is it just that I've been lucky for the past 11 years to experience respect as a histotech? I, for one, would NOT join a national walk-out for that reason. It's belief in patient care, the science and the art that I love so much about histology. I know doctors in this area respect their techs. Sincerely, Deborah King, HT(ASCP) Histology Supervisor Sacramento, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ThisisAnn <@t> aol.com Fri Apr 1 13:16:20 2005 From: ThisisAnn <@t> aol.com (ThisisAnn@aol.com) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Position in NJ Message-ID: <155.4dcb2b4c.2f7ef804@aol.com> Laboratory Corporation of America, located in Raritan, NJ, has an immediate opening for a Histotechnician/technologist. Some experience preferred. Position includes grossing, embedding, cutting and, routine/special staining. Please contact Ann Angelo at 1-800-524-0249 x2912 and fax your resume to 908-927-1129 From Jackie.O'Connor <@t> abbott.com Fri Apr 1 13:37:35 2005 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] National Histology Walkout Message-ID: I don't get the reference to Chicago - I live 50 NW of Chicago, but we still say we're from Chicago. What did I miss? "Bonner, Janet" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/01/2005 01:08 PM To: "'Robyn Vazquez '" , "'histonet-bounces@lists.utsouthwestern.edu '" , "'WWmn916@aol.com '" , "'histonet@lists.utsouthwestern.edu '" cc: Subject: RE: [Histonet] National Histology Walkout There was a message yesterday, but I honestly believe it was in jest. Unless you live in Chicago, IL, there isn't anywhere to "walk" out of!! - -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: WWmn916@aol.com; histonet@lists.utsouthwestern.edu Sent: 3/31/2005 4:44 PM Subject: Re: [Histonet] National Histology Walkout Deborah, Did I miss something? Who said they were going to walk out? From what I have read they are going to boycott not renewing their ASCP stickers. Did I miss a message? Robyn >>> 03/31/05 12:56 PM >>> Wow, I must be living in a bubble in the Sacramento area. I personally don't feel victimized or taken advantage of in this profession. Is it just that I've been lucky for the past 11 years to experience respect as a histotech? I, for one, would NOT join a national walk-out for that reason. It's belief in patient care, the science and the art that I love so much about histology. I know doctors in this area respect their techs. Sincerely, Deborah King, HT(ASCP) Histology Supervisor Sacramento, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gliuygao <@t> hotmail.com Fri Apr 1 13:50:34 2005 From: gliuygao <@t> hotmail.com (yan gao) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] (no subject) Message-ID: Dear Histonet, I had some trouble stain pERK antibody and monoclone pMEK antibody on xenograft melanoma tumor. Can anyone share some tips for me. I did try cell signaling technology #4376 & 3910. I see alittle stain on #4376. but weeks. Thanks. Best Regards, Yan Novartis Institute for BioMedical Research From mainlinemohs <@t> yahoo.com Fri Apr 1 13:52:22 2005 From: mainlinemohs <@t> yahoo.com (Michael Lehrer) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Position available Philadelphia suburbs Message-ID: <20050401195222.60163.qmail@web31104.mail.mud.yahoo.com> Hello histonetters- I'm expanding a Mohs practice in the Philadelphia suburbs and need a histotech to work with me. Someone with Mohs experience would be great, but we could easily teach the nuances of Mohs to anyone with histotech skills. Salary would be top notch, and, all benefits would be included. (Those not needing benefits could trade them for a higher salary!) This would ideally be a full time position, but I would also consider hiring 2 part timers. I look forward to hearing from anyone interested. Thanks, Michael S. Lehrer, MD mainlinemohs@yahoo.com --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From gliuygao <@t> hotmail.com Fri Apr 1 13:54:16 2005 From: gliuygao <@t> hotmail.com (yan gao) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] ERk antibody In-Reply-To: Message-ID: >From: "yan gao" >To: histonet@lists.utsouthwestern.edu, histonet@list.utsouthwestern.edu >Subject: [Histonet] (no subject) >Date: Fri, 01 Apr 2005 14:50:34 -0500 > > > > Dear Histonet, > > I had some trouble stain pERK antibody and monoclone pMEK >antibody on > xenograft melanoma tumor. Can anyone share some tips for me. I >did try > cell signaling technology #4376 & 3910. I see waek stain on >#4376. Thanks. > > > Best Regards, > > Yan > > Novartis Institute for BioMedical Research >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Fri Apr 1 14:08:53 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] National Histology Walkout Message-ID: Janet, I belong to a union. If they were to strike then I would be expected to walk out. BUT!!! As bad as it sounds...I would cross the picket line, because the patients that I serve have dermatological cancer and that's way more important than $$$...I am paid good already. I just wish they gave us more vacation though as always... Robyn OHSU From RCazares <@t> schosp.org Fri Apr 1 14:21:39 2005 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: <913FAC2B773C19488E26AE6572180FA501982248@exch01.schosp.org> Hi all, I just have to add my opinion to what is going on, on the Histo net. Don't department heads have a say in what the qualifications should be for tech positions? Maybe it is time to get together with the powers that be and make a change. ASCP has raised the education level for certifying histo-techs, this should be an opportunity to get together with your administrators and make requirement changes for your Histo tech positions. That is exactly what I am going to do. If your institution prefers to hire uncertified people to do the work certified techs should be doing, is this a place you really want to work? Everyone deserves to be treated with respect and deserves to know that their hard work is appreciated. If you don't get this where you are at, leave. There are a lot of other places in need of histo-techs. After my job was phased out at a hospital where I had worked for 10 years, I went to work at 3 different labs before I found my niche. We spend too much of our daily lives at our jobs, we don't need to be unhappy and with high blood pressure the minute we walk into our lab. Life is too short. And as far as Continuing Education, NSH offers online CE and teleconferences (about 8 or 9 a year ). Leica and Thermoshandon (is that their name now?) as I'm sure other vendors do also, offer 1 day workshops for CEU's . The continuing education for our field is out there, sometimes we just have to look for it. P.S. I'll have a position open in December, anybody interested? Lots of Love to all my Histo brothers and sisters, Ruth Cazares HT (ASCP) Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From pmarcum <@t> vet.upenn.edu Fri Apr 1 14:22:02 2005 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Linda Durbin Exackt Message-ID: <1112386922.424dad6a2d7f9@imp.vet.upenn.edu> Hi All, Sorry to bother the list with this however, I am attempting to reach Linda Durbin. Linda if you see this or someone lets you know please call Pam Marcum at 610-925-6278. I am at the University of PA Vet School and need to speak with you. Pam Marcum From kelly.mcqueeney <@t> bms.com Fri Apr 1 14:40:39 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: ASCP - My 2 cents In-Reply-To: <913FAC2B773C19488E26AE6572180FA501982248@exch01.schosp.org> References: <913FAC2B773C19488E26AE6572180FA501982248@exch01.schosp.org> Message-ID: <424DB1C7.6030503@bms.com> I have no idea what level of education a qualified histotech must have, can someone enlighten me? Thanks, Kelly Cazares, Ruth wrote: >Hi all, > > > >I just have to add my opinion to what is going on, on the Histo net. >Don't department heads have a say in what the qualifications should be >for tech positions? Maybe it is time to get together with the powers >that be and make a change. ASCP has raised the education level for >certifying histo-techs, this should be an opportunity to get together >with your administrators and make requirement changes for your Histo >tech positions. That is exactly what I am going to do. If your >institution prefers to hire uncertified people to do the work certified >techs should be doing, is this a place you really want to work? >Everyone deserves to be treated with respect and deserves to know that >their hard work is appreciated. If you don't get this where you are at, >leave. There are a lot of other places in need of histo-techs. > > > >After my job was phased out at a hospital where I had worked for 10 >years, I went to work at 3 different labs before I found my niche. We >spend too much of our daily lives at our jobs, we don't need to be >unhappy and with high blood pressure the minute we walk into our lab. >Life is too short. > > > >And as far as Continuing Education, NSH offers online CE and >teleconferences (about 8 or 9 a year ). Leica and Thermoshandon (is >that their name now?) as I'm sure other vendors do also, offer 1 day >workshops for CEU's . The continuing education for our field is out >there, sometimes we just have to look for it. > > > >P.S. I'll have a position open in December, anybody interested? > > > > > >Lots of Love to all my Histo brothers and sisters, > > > > > >Ruth Cazares HT (ASCP) > >Department of Pathology > >Swedish Covenant Hospital > >Chicago, IL 60625 > > > > > >*** Confidentiality Statement *** >This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. > > >Thank you for your cooperation. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Janet.Bonner <@t> FLHOSP.ORG Fri Apr 1 14:42:05 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] National Histology Walkout Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4209@fh2k093.fhmis.net> ASCP Headquarters used to be in Chicago, IL -----Original Message----- From: Jackie M O'Connor [mailto:Jackie.O'Connor@abbott.com] Sent: Friday, April 01, 2005 2:38 PM To: Bonner, Janet Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] National Histology Walkout I don't get the reference to Chicago - I live 50 NW of Chicago, but we still say we're from Chicago. What did I miss? "Bonner, Janet" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/01/2005 01:08 PM To: "'Robyn Vazquez '" , "'histonet-bounces@lists.utsouthwestern.edu '" , "'WWmn916@aol.com '" , "'histonet@lists.utsouthwestern.edu '" cc: Subject: RE: [Histonet] National Histology Walkout There was a message yesterday, but I honestly believe it was in jest. Unless you live in Chicago, IL, there isn't anywhere to "walk" out of!! - -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: WWmn916@aol.com; histonet@lists.utsouthwestern.edu Sent: 3/31/2005 4:44 PM Subject: Re: [Histonet] National Histology Walkout Deborah, Did I miss something? Who said they were going to walk out? From what I have read they are going to boycott not renewing their ASCP stickers. Did I miss a message? Robyn >>> 03/31/05 12:56 PM >>> Wow, I must be living in a bubble in the Sacramento area. I personally don't feel victimized or taken advantage of in this profession. Is it just that I've been lucky for the past 11 years to experience respect as a histotech? I, for one, would NOT join a national walk-out for that reason. It's belief in patient care, the science and the art that I love so much about histology. I know doctors in this area respect their techs. Sincerely, Deborah King, HT(ASCP) Histology Supervisor Sacramento, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Fri Apr 1 15:18:19 2005 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: <566FB0B522443D43AF02D2ADBE35A6F00163F468@UTHEVS3.mail.uthouston.edu> Ruth I can see where you are coming from but I do not agree with this concept. It is fine for individual histotechs to try to get as much salary as possible from their current employer, good luck to y'all for this. However this does not change the overall situation for histotechs in general and depends on a one on one interaction or confrontation. The concept of the squeaky wheel springs to mind. What is desperately needed is a uniform salary base for histotechs depending on their level, certification etc. This does not mean that salaries would have to be the same for all regardless of their location, but there would be a baseline for all that all employers would have to abide by. We all recognize that some places are simply more expensive to live at than others. What is needed is a base scale with appropriate adjustments for location and for each stage of advancement in the profession. If expertise and salaries are tied together and for all defined positions there is a requirement (for HIPPA or similar regulations) that in order to perform certain tests you have certification, then salary should follow. A united approach is, I believe, the only way to ensure our profession maintains its standards of excellence and also is recognized for its contributions to the health of patients. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth Sent: Friday, April 01, 2005 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: ASCP - My 2 cents Hi all, I just have to add my opinion to what is going on, on the Histo net. Don't department heads have a say in what the qualifications should be for tech positions? Maybe it is time to get together with the powers that be and make a change. ASCP has raised the education level for certifying histo-techs, this should be an opportunity to get together with your administrators and make requirement changes for your Histo tech positions. That is exactly what I am going to do. If your institution prefers to hire uncertified people to do the work certified techs should be doing, is this a place you really want to work? Everyone deserves to be treated with respect and deserves to know that their hard work is appreciated. If you don't get this where you are at, leave. There are a lot of other places in need of histo-techs. After my job was phased out at a hospital where I had worked for 10 years, I went to work at 3 different labs before I found my niche. We spend too much of our daily lives at our jobs, we don't need to be unhappy and with high blood pressure the minute we walk into our lab. Life is too short. And as far as Continuing Education, NSH offers online CE and teleconferences (about 8 or 9 a year ). Leica and Thermoshandon (is that their name now?) as I'm sure other vendors do also, offer 1 day workshops for CEU's . The continuing education for our field is out there, sometimes we just have to look for it. P.S. I'll have a position open in December, anybody interested? Lots of Love to all my Histo brothers and sisters, Ruth Cazares HT (ASCP) Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Fri Apr 1 15:26:19 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: Kelly, I have been doing histology for going on 15 years (?) and never certified as my own choice. I do EXCELLENT QUALITY work. I was taught in the military as OTJ training. Robyn From Charles.Embrey <@t> carle.com Fri Apr 1 16:06:48 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: Barry, You hit the nail right on the head: "If expertise and salaries are tied together and for all defined positions there is a requirement (for HIPPA or similar regulations) that in order to perform certain tests you have certification, then salary should follow." Regulations you speak of are more like CLIA '88, CAP guidelines and licensure. Except for a few states, like Florida, anyone can fully function as a histotech without any type of certification. Many states are now addressing the licensure issue. The lab professions in Illinois (my state) are pushing for legislation but the histology professionals in the state requested exemption from the bill because they were afraid of a negative impact. Licensure is great for those that are able to be licensed (certified) but can be a nightmare for those that have chosen to work in the field for many years (I think Robyn says she has been working 15) without certification that now suddenly find themselves legislated out of a job. Pathologists and lab managers also feel the pinch in having to search for certified people and the heartache of having to terminate loyal employees because they don't meet the licensing requirements. In the 25 years I have been in the field I have seen pay and benefits increase more so on the upper limits but not so much in the starting range. Licensure can be a double edge sword. We just have to remember to be prepared and duck when we see it coming. Charles Embrey PA(ASCP), HT(ASCP) Urbana IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Friday, April 01, 2005 3:18 PM To: histonet Subject: RE: [Histonet] Re: ASCP - My 2 cents Ruth I can see where you are coming from but I do not agree with this concept. It is fine for individual histotechs to try to get as much salary as possible from their current employer, good luck to y'all for this. However this does not change the overall situation for histotechs in general and depends on a one on one interaction or confrontation. The concept of the squeaky wheel springs to mind. What is desperately needed is a uniform salary base for histotechs depending on their level, certification etc. This does not mean that salaries would have to be the same for all regardless of their location, but there would be a baseline for all that all employers would have to abide by. We all recognize that some places are simply more expensive to live at than others. What is needed is a base scale with appropriate adjustments for location and for each stage of advancement in the profession. If expertise and salaries are tied together and for all defined positions there is a requirement (for HIPPA or similar regulations) that in order to perform certain tests you have certification, then salary should follow. A united approach is, I believe, the only way to ensure our profession maintains its standards of excellence and also is recognized for its contributions to the health of patients. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth Sent: Friday, April 01, 2005 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: ASCP - My 2 cents Hi all, I just have to add my opinion to what is going on, on the Histo net. Don't department heads have a say in what the qualifications should be for tech positions? Maybe it is time to get together with the powers that be and make a change. ASCP has raised the education level for certifying histo-techs, this should be an opportunity to get together with your administrators and make requirement changes for your Histo tech positions. That is exactly what I am going to do. If your institution prefers to hire uncertified people to do the work certified techs should be doing, is this a place you really want to work? Everyone deserves to be treated with respect and deserves to know that their hard work is appreciated. If you don't get this where you are at, leave. There are a lot of other places in need of histo-techs. After my job was phased out at a hospital where I had worked for 10 years, I went to work at 3 different labs before I found my niche. We spend too much of our daily lives at our jobs, we don't need to be unhappy and with high blood pressure the minute we walk into our lab. Life is too short. And as far as Continuing Education, NSH offers online CE and teleconferences (about 8 or 9 a year ). Leica and Thermoshandon (is that their name now?) as I'm sure other vendors do also, offer 1 day workshops for CEU's . The continuing education for our field is out there, sometimes we just have to look for it. P.S. I'll have a position open in December, anybody interested? Lots of Love to all my Histo brothers and sisters, Ruth Cazares HT (ASCP) Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Fri Apr 1 16:31:34 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: <20050401223134.65333.qmail@web52610.mail.yahoo.com> Well get out the violins I'm tearing up here. Why would you choose not to get certified if you "love" your job so much. If you've been in the field that long it would be a snap to get certified and besides they wouldn't be firing anyone if they made licensure mandatory, it would have to begin at a future date, everyone else would be grandfathered in. As far as pathologists not being able to find qualified people, they are partly to blame, they don't want mandatory licensure. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Jackie.O'Connor <@t> abbott.com Fri Apr 1 16:50:08 2005 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] National Histology Walkout Message-ID: I love my job. I love my job. I love my job. I'm paid what I consider a fair wage - after all, I agreed to the salary before I took this job - didn't we all? I agreed to the benefits, I agreed to the terms. No one made me take this job, and no one makes me stay here. I'm good at what I do, and my bosses appreciate, respect, and reward that. I've been in this business since 1970 - ASCP since 1971 - quit paying my ASCP dues around 1980, I guess. I lost my certificate with all the little stickers - the ASCP told me I would have to re-pay for all the lost stickers. So - no more stickers. I've had 3 really great jobs in 35 years, and about 15 that just plain sucked - but I've never had to stay somewhere I didn't like. I followed my Navy husband around the country for 20 of those years, so I was never able to work anywhere more than 3 years at a time - but I never EVER had trouble finding a good histology job where I was treated fairly. I DID turn down many jobs that just didn't pay well, or had horrible working conditions. I once turned down a good paying job at a world famous zoo because I was disgusted with the appearance of the working conditions behind the scenes. I wasn't working for the love of histology - I was a Navy wife trying to supplement my husbands crummy income to raise 4 kids. When he was deployed, and I was left alone with those 4 kids, the hospital I was working at ( a good plug for Sharp Memorial in San Diego) allowed me to adjust my schedule to accommodate what I needed to do to handle my kids. I've also learned that not every organization is willing to listen to an unhappy employee. If your boss is keeping his boss happy, they really don't care if he's "mean" to you. I remember going to HR at one place because my boss showed up for work apparently drunk - HR asked me "What do you want us to do about it?" I worked in another place where the pathologist insisted she knew secrets about Elvis' death. Worked another place where one of the pathologists was arrested for selling gold from full mouth dental extractions. I've got a million of them. I also worked at a hospital (1970's) where all the lab workers went out on strike (I was a scab - I was sent there to fill in from another hospital where I was employed - I didn't know the meaning of the word scab then). The hospital fired ever single person who went on strike. Sometimes the light just has to come on before you realize there aren't many Nirvana's out there. I still have to work - I've still got 3 kids to finish college - I know that if I lost this job tomorrow, I'd have to get another one -but I was looking for a job when I found this one. What do you want from the ASCP? They haven't done anything for me in 25 years - anything I've learned I've done on my own - and I've done well (I think). "Robyn Vazquez" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/01/2005 02:08 PM To: WWmn916@aol.com, Janet.Bonner@FLHOSP.ORG, histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu cc: Subject: RE: [Histonet] National Histology Walkout Janet, I belong to a union. If they were to strike then I would be expected to walk out. BUT!!! As bad as it sounds...I would cross the picket line, because the patients that I serve have dermatological cancer and that's way more important than $$$...I am paid good already. I just wish they gave us more vacation though as always... Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Fri Apr 1 17:17:37 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] National Histology Walkout Message-ID: Jackie, Your a gold mine:0) From rnewlin <@t> burnham.org Fri Apr 1 18:44:46 2005 From: rnewlin <@t> burnham.org (Robin Newlin) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] National Histology Walkout References: Message-ID: <001901c5371d$2c154d60$930d10ac@RNEWLIN> VERY WELL PUT!!!!!! ----- Original Message ----- From: "Jackie M O'Connor" To: "Robyn Vazquez" Cc: Sent: Friday, April 01, 2005 2:50 PM Subject: RE: [Histonet] National Histology Walkout > I love my job. I love my job. I love my job. I'm paid what I consider a > fair wage - after all, I agreed to the salary before I took this job - > didn't we all? > I agreed to the benefits, I agreed to the terms. No one made me take this > job, and no one makes me stay here. I'm good at what I do, and my bosses > appreciate, respect, and reward that. > I've been in this business since 1970 - ASCP since 1971 - quit paying my > ASCP dues around 1980, I guess. I lost my certificate with all the little > stickers - the ASCP told me I would have to re-pay for all the lost > stickers. So - no more stickers. I've had 3 really great jobs in 35 > years, and about 15 that just plain sucked - but I've never had to stay > somewhere I didn't like. I followed my Navy husband around the country > for 20 of those years, so I was never able to work anywhere more than 3 > years at a time - but I never EVER had trouble finding a good histology > job where I was treated fairly. I DID turn down many jobs that just > didn't pay well, or had horrible working conditions. I once turned down a > good paying job at a world famous zoo because I was disgusted with the > appearance of the working conditions behind the scenes. I wasn't working > for the love of histology - I was a Navy wife trying to supplement my > husbands crummy income to raise 4 kids. When he was deployed, and I was > left alone with those 4 kids, the hospital I was working at ( a good plug > for Sharp Memorial in San Diego) allowed me to adjust my schedule to > accommodate what I needed to do to handle my kids. > I've also learned that not every organization is willing to listen to an > unhappy employee. If your boss is keeping his boss happy, they really > don't care if he's "mean" to you. I remember going to HR at one place > because my boss showed up for work apparently drunk - HR asked me "What do > you want us to do about it?" I worked in another place where the > pathologist insisted she knew secrets about Elvis' death. Worked another > place where one of the pathologists was arrested for selling gold from > full mouth dental extractions. I've got a million of them. I also worked > at a hospital (1970's) where all the lab workers went out on strike (I was > a scab - I was sent there to fill in from another hospital where I was > employed - I didn't know the meaning of the word scab then). The hospital > fired ever single person who went on strike. > Sometimes the light just has to come on before you realize there aren't > many Nirvana's out there. > I still have to work - I've still got 3 kids to finish college - I know > that if I lost this job tomorrow, I'd have to get another one -but I was > looking for a job when I found this one. > What do you want from the ASCP? They haven't done anything for me in 25 > years - anything I've learned I've done on my own - and I've done well (I > think). > > > > > > "Robyn Vazquez" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 04/01/2005 02:08 PM > > > To: WWmn916@aol.com, Janet.Bonner@FLHOSP.ORG, > histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > cc: > Subject: RE: [Histonet] National Histology Walkout > > > Janet, > I belong to a union. If they were to strike then I would be expected to > walk out. BUT!!! As bad as it sounds...I would cross the picket line, > because the patients that I serve have dermatological cancer and that's > way more important than $$$...I am paid good already. I just wish they > gave us more vacation though as always... > > Robyn > OHSU > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From katri <@t> cogeco.ca Fri Apr 1 20:59:47 2005 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] email problems Message-ID: <001301c53730$07bc0e50$6a9a9618@Katri> A big Thank You to all the kind souls, that tried to contact me after my plea of help! I finally figured out, that my Spam killer is hogging all my mails, neatly dividing them into two categories: Blocked and Accepted ones, but never letting most of them through to my Outlook inbox. Bizarre!!!!! I now have over 1600 messages to go through and most of them from my Histonet friends! Katri Katri Tuomala Hamilton, Ontario, Canada From millicent_renee <@t> yahoo.com Sat Apr 2 09:33:32 2005 From: millicent_renee <@t> yahoo.com (Millicent Bruce) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] mart stain Message-ID: <20050402153332.18086.qmail@web31503.mail.mud.yahoo.com> Hi! I'm work for a plastic surgeon who has recently completed a fellowship on the Mohs procedure. He has been working as a Mohs surgeon for about 10 months. We are all still new at this. He has mentioned something about a mart stain. Is there anybody that can help me? I need to know how to do it. If anybody can tell me how, or atleast let me know where I can find some information about it would be helpful. Thank you very much, Millicent Bruce __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From pruegg <@t> ihctech.net Sat Apr 2 10:52:14 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] participation In-Reply-To: <001a01c52ff2$c2b01d40$29d1df42@TARANGO> Message-ID: <200504021652.j32GqscS088263@pro12.abac.com> I must chime in here a bit. Those who do not participate in the their professional organizations (ASCP, NSH, state societies, etc.) are missing out on resources that they of course don't know anything about because they are not engaged. Even when you do pay ASCP dues each year the benefits are not always transparent. ASCP is the Certifying agency for our profession, without them none of us would have documentation that we are "professionals". I know that there are plenty of people out there who may have been working in the field for a long time and can perform the required tasks, but the fact that they have not gotten certified says something about them that frankly would not impress me as a perspective employer. With a few exceptions (ie Jackie O, who is certified I know) I would be willing to bet that these non-certified, non-engaged people do not read the Journals or attend classes in their field, which again would not impress me as their employer. It is our job to educate the administrators. They often have no idea that non-certified people are working for them or even that certification is available in some cases. NSH has been working for many years to try and get CAP to require certified Histotech's be working in the histo lab, we even asked that they at least require supervisors be certified. CAP still has no such requirements. It can be a long tuff battle, but we are making progress. ASCP, NSH, CAP and State Histology Societies, to mention a few, are our professional bargaining agencies. Through them we will make the changes in the field we want to see. Without these agencies we are just individuals whining about issues we are doing nothing to affect. Best regards, Patsy Ruegg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Wednesday, March 23, 2005 2:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 17, Issue 1 I want to know why the ASCP is refusing to tell me what my certification number is. I pass their ridiculous test 1st try. I don't pay their dues every year because it's a ripoff. While I'm here in Alsaka, I need to know my number. I have it written down back home, but I'm not there now. They'll only tell me my number if i pay them their yearly fees. I passed the exam, so why are they bullying me here? Maybe if they worked a little harder on our wages or getting us some respect in the lab, I could afford it or wouldn't mind paying it. Mark Tarango > Message: 9 > Date: Thu, 31 Mar 2005 20:30:39 -0500 > From: > Subject: [Histonet] ASCP BOR - answers to questions > To: "Histonet" > Message-ID: <00b201c5365a$6a3d83a0$50d7d445@domainnotset.invalid> > Content-Type: text/plain; charset="iso-8859-1" > > Going to try to answer several questions in this one email. If not > interested in ASCP BOR or in some answers - please push the delete button > now. > > 1. WHO TO TALK TO ABOUT COMPLAINTS ABOUT ASCP BOARD OF REGISTRY (BOR) > > The NSH appointed representative to ASCP BOR is Marilyn Gamble. There is a > link to her email at the NSH web site > > http://www.nsh.org > - click on Who We Are > - click on Appointments > - click on Marilyn's email link under ASCP BOR > > The list of histotechs and pathologists on the ASCP BOR Histotechnology > exam > committee can be found on the ASCP BOR webpage. There are no email links > to > their names. > > http://www.ascp.org/bor/about > - click on Histotechnology Examination Committee > > Also, you can contact ASCP BOR directly via phone or email. You could ask > them a question, or ask for the phone number on the Histotechnology exam > committee. The list of staff people working at the BOR and their roles > can > be found on: > > http://www.ascp.org/bor/about/ > - at the bottom of the page, click on Contact an ASCP Board of Registry > staff member. > > > 2. WHY THE INCREASE IN THE COST OF GRADING HT/HTL PRACTICAL EXAMS? > > Well, the ASCP BOR staff member was at NSH S/C in Toronto, and talked > about > it at the booth, and at several committee meetings. I also called ASCP BOR > in the fall for additional information, which they gave out. Nothing > hidden > or secret about it. Ask, and they will tell. > > The HT and HTL exams are the most expensive technician/technologist exams > for ASCP BOR, because HT/HTL/pathologists graders have to be brought in > from > all other the USA, two weekends a year, to grade all the sets. > > The fee paid by the HT/HTL candidates did not cover the cost of the ASCP > grading the written (computer) and the practical HT/HTL exams. > > ASCP BOR had made several changes recently, to keep the cost down. > > Instead of submitting 15 slides, BOR statistically found that 9 slides > were > sufficient to achieve the same pass/fail rate as 15 slides. (In other > words, > if someone does a good job on cutting and staining 9, they would have done > the same good job on 15. And the same with someone doing a not so good/bad > job.) By reducing the number of slides, they reduced the number of people > needed to grade the slides, thereby reducing costs. > > A new change is that the candidate must pass the written/computer portion > first, before they can submit their practical. This will reduce the number > of practicals being sent in. This again will reduce the number of graders > needed, which will keep costs down. > > However, unfortunately, these changes could not keep the costs down > enough, > and now ASCP BOR is asking the candidates to help to cover SOME of the > costs > of grading the practical exams, by paying an additional $75. Notice the > word > SOME. This additional fee still does not cover all the costs. > > > 3. WHAT DO I, AS A HISTOTECH, GET BY BEING AN ASCP MEMBER? > > Maybe not as much as being an NSH member, but I still think I get some > things. > - Tech Samples (for continuing education) > - ASCP Teleconferences (at least 3 each quarter are histology. Others on > management are also helpful) > - "Laboratory Medicine" (which might not have as much histology articles > as > I might like, but it gives me a better understanding of how all the labs > fit > in together. Helpful in management issues.) > - Representatives from ASCP in Washington DC, working on: laws for payment > and reimbursement which effect my pay; getting grants to start new lab > schools; etc. > - Books like the Frieda Carson textbook, HT/HTL exam study books, Jules > Elias book on IHC > - Annual wage and salary survey > > And all of these things cost money to set up and run. Money from ASCP > members. > > All of the above information I obtained by A) calling ASCP BOR and asking > them questions I have, B) attending committee meetings at NSH where the > ASCP > BOR staff are there to answer questions, and C) talking with the ASCP BOR > staff that are at the ASCP BOR booth in the exhibit hall that the NSH S/C, > D) reading the ASCP BOR web page, "Laboratory Medicine", and anything else > I > can get. It's not a secret. > > And calling does help. Sometimes it takes a while, but things do change. > I've called the NSH representatives to ASCP BOR and the chair of the ASCP > BOR Histotechnology exam committee in the past with concerns, ideas, > suggestions, questions. I've gotten answers, and often they have > incorporated my suggestions into changing things on the exams (different > tissues, stains, checking on a question when my students swear there > wasn't > a right answer (typo, it turned out, which the BOR QC had already caught > so > the students' scores were not effected, etc.)). I've raised questions > about > the wage and salary survey, and that was modified. I've offered > suggestions > about their flyers about histotechs, and these were changed in the next > revision (about 4 years later - slowly, remember?). No, they didn't change > these things because it was ME who called. They changed because I offered > a > good suggestion. There are also things they did not change. So it goes. > > No, it isn't perfect. But from where I stand, I think they need more > constructive input and more people being involved. That's us, people. We > care about our field, otherwise we wouldn't still be in the field and on > Histonet. So let's be constructive and offer concrete suggestions and > ideas > for improvement to our representatives - email, call, and attend the NSH > Education Committee or Instructors in Histotechnology meeting in Fort > Lauderdale. It takes more than 6 people sitting in on a committee, to > make > changes. It's sad when the meetings are open to everyone of the 1200 > people > in attendance, and only 6 people show up. (That is my personal opinion, > not > something gleaned from a book or webpage.) > > Off my soap box for a while. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sat Apr 2 11:06:44 2005 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] ASCP BOR - answers to questions References: <20050401164419.92672.qmail@web52608.mail.yahoo.com> Message-ID: <001301c537a6$595f16f0$7929f318@yourxhtr8hvc4p> As a clinical instructor for the histology program here in San Antonio, I always emphasize education and taking the registry. Since opening PRL in 2000, all of my techs are certified or are eligible. The incentive to take and pass the board is a $2.00 an hour pay raise and a refund of the exam fees. All my board eligible techs have rotated through PRL. so I know they can do he job. Those who fail to at least attempt to take the registry are held at their current pay rate and they know it. it is also annotated on their annual evaluations. The three techs that are eligible have applied and the only tech not eligible is a student. I have always encouraged taking the registry, even when I was in the military. My beef is not with the exam, it's with ASCP. Since they are the only governing board for histology, we are stuck with their rules, regulations and prices. At least MTs and MLTs have another avenue to get registered, albeit it might not be as well recognized or well accepted as the ASCP, they still have another avenue. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Larry Woody" To: Sent: Friday, April 01, 2005 10:44 AM Subject: Re: [Histonet] ASCP BOR - answers to questions > Key words there "little known by the general public", is exactly why > places get away with hiring unqualified people in labs. I don't know where > most of the people who respond on this list are working but if you haven't > worked in a lab with unqualified people then in my opinion you've been > very lucky. When a lab hires someone who doesn't have the degree or the > BOR and they are considered the same level it is demeaning and it's just > wrong and what has the ASCP done about it. This profession is in the > closet and it's high time someone brought it out to the public. Larry > > > --------------------------------- > Do you Yahoo!? > Yahoo! Mail - 250MB free storage. Do more. Manage less. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Anti-Virus. > Version: 7.0.308 / Virus Database: 266.9.1 - Release Date: 4/1/2005 > > From Krat18 <@t> aol.com Sat Apr 2 12:26:24 2005 From: Krat18 <@t> aol.com (Krat18@aol.com) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Who does the immunos? Message-ID: What policy do most labs have about letting their techs do immunos? Are all the techs trained to do them, or are only the registered techs trained to do them, or do only a limited number of techs do them? Our program is fairly new, and there is a discussion among the pathologists and administration and techs about what is the best policy. I'd like to hear from other labs about their procedures. Thanks in advance. Karen _krat18@aol.com_ (mailto:krat18@aol.com) From mainlinemohs <@t> yahoo.com Sat Apr 2 14:58:34 2005 From: mainlinemohs <@t> yahoo.com (Michael Lehrer) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] mart stain In-Reply-To: 6667 Message-ID: <20050402205834.17001.qmail@web31108.mail.mud.yahoo.com> Here's the reference. Hope this helps. -Michael S. Lehrer, MD -Mohs Micrographic Surgery -www.mainlinemohs.com Dermatol Surg. 2004 Mar;30(3):403-8.Related Articles, Links Immunostaining melanoma frozen sections: the 1-hour protocol. Bricca GM, Brodland DG, Zitelli JA. BACKGROUND: There is significant debate over the use of frozen section processing in Mohs micrographic surgery (MMS) for melanoma. Opponents argue that individual melanocytes are too subtle to view consistently on frozen sections. On the other hand, proponents state that (1) melanocytes are visible on well-prepared frozen sections and (2) MMS using frozen sections for evaluation of melanoma surgical margins achieves comparable recurrence rates when compared with MMS using paraffin-embedded, permanent sections. OBJECTIVE: To introduce a new immunohistochemical (IHC) staining protocol that consistently produces melanoma frozen section slides in 1 hour that are easily evaluated during MMS. METHODS: We adapted a polymer-based IHC staining protocol to use with MMS frozen sections for the evaluation of melanoma surgical margins. RESULTS: When used with antibody directed against MART-1 for frozen section evaluation of melanoma, the section staining is reproducible and specific for melanocytes. CONCLUSIONS: In contrast to current IHC protocols that are time consuming (2 to 2.5 hours), we present a new frozen section protocol that takes approximately 1 hour to perform. This technique benefits patients, histotechnicians, and surgeons Millicent Bruce wrote: Hi! I'm work for a plastic surgeon who has recently completed a fellowship on the Mohs procedure. He has been working as a Mohs surgeon for about 10 months. We are all still new at this. He has mentioned something about a mart stain. Is there anybody that can help me? I need to know how to do it. If anybody can tell me how, or atleast let me know where I can find some information about it would be helpful. Thank you very much, Millicent Bruce --------------------------------- Do you Yahoo!? Yahoo! Mail - You care about security. So do we. From GODSGIRLNOW <@t> msn.com Sat Apr 2 16:29:00 2005 From: GODSGIRLNOW <@t> msn.com (Roxanne Soto) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Who does the immunos? References: Message-ID: Karen, This would depend on your state and you institution. Many institutions only allow technologists to do them. Some states will aonly allow Technologists to do them. If this is a brand new job description, then it can be incorporated into the job description. If it is an existng description, review it and see what it says, you can always add it to the current job description. I would recommend that it be a separate job description for the IHC Tech, because it takes a special kind of tech to be able to handle all of the stress and math and chemistry required to work up all of them antibodies and be able to troubleshoot them as well as being able to help the pathologists with their questions. For example, the antibody CEA--there is a monoclonal and a polyclonal antibody for this and the pathologist called me and asked for CEA and I asked hime if he wanted monoclonal or polyclonal and he asked me what the difference is. The person doing the IHC will have to have the knowledge to tell him that since he was ordering it on a liver biopsy and he was looking for cancer originating from the colon, what he probably wanted was CEA polyclonal, as this antibody is for liver metasasis from colonic cancers, and he probably wanted a hepar done as well. Let me know if you need any other help----be glad to offer my 2 cents any time Roxanne Soto ----- Original Message ----- From: Krat18@aol.com To: histonet@lists.utsouthwestern.edu Sent: Saturday, April 02, 2005 1:26 PM Subject: [Histonet] Who does the immunos? What policy do most labs have about letting their techs do immunos? Are all the techs trained to do them, or are only the registered techs trained to do them, or do only a limited number of techs do them? Our program is fairly new, and there is a discussion among the pathologists and administration and techs about what is the best policy. I'd like to hear from other labs about their procedures. Thanks in advance. Karen _krat18@aol.com_ (mailto:krat18@aol.com) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pex0220 <@t> yahoo.com.cn Sun Apr 3 09:11:03 2005 From: pex0220 <@t> yahoo.com.cn (=?gb2312?q?=CC=EC=20=D0=C1?=) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Problems about Tween 20 Message-ID: <20050403141103.72660.qmail@web15510.mail.cnb.yahoo.com> Hello, all, I checked some data , which showed that Tween 20 could make bone sections flatten. But I am not sure of contrations(1%,2%,5%), time of incubation. If you have some experience in it , do you do me a favor? Thank you evey much! Guofeng Qian --------------------------------- Do You Yahoo!? ×¢²áÊÀ½çÒ»Á÷Æ·ÖʵÄÑÅ»¢Ãâ·ÑµçÓÊ From stewart_georgia <@t> sbcglobal.net Sun Apr 3 09:54:41 2005 From: stewart_georgia <@t> sbcglobal.net (Georgia Stewart) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Who does the immunos? 2-cents more In-Reply-To: 6667 Message-ID: <20050403145441.85631.qmail@web81403.mail.yahoo.com> Roxanne, What you said is very true, but unfortunately in the laboratory where I worked the pathologists didn't function in this way. The supervisor is certified HT but her only continuing education has been the 5 days she spent at Ventana learing how to use their automated IHC stainer. California does not license HTs or HTLs and as far as I know there is no requirement that a tech be certified before they can perform IHC in California. One lab I'm familiar with, only allows the med techs to perform the IHC (on an automated stainer) because they are "educated and licensed" and don't allow the HTs to perform any IHC. Don't think anyone at this facility is HTL certified. ASCP is some of the problem, but I feel it is also, in part, a result of the pathologists not wanting to recognize the ongoing need for continuing education, and not just in IHC. The lab offered no incentive for continuing education, either in monetary assistance to attend seminars/state histology meetings, etc., or any increase in salary because of continuing education paid for by the tech. The only ones who received monetary assistance were the cytotechs who are required to have continuing education. The cytotechs are licensed by the state of California and they are also ASCP certified. In this lab, licensing was the motivating factor for the pathologists to pay for the cytotechs continuing education. Georgia Roxanne Soto wrote: Karen, This would depend on your state and you institution. Many institutions only allow technologists to do them. Some states will aonly allow Technologists to do them. If this is a brand new job description, then it can be incorporated into the job description. If it is an existng description, review it and see what it says, you can always add it to the current job description. I would recommend that it be a separate job description for the IHC Tech, because it takes a special kind of tech to be able to handle all of the stress and math and chemistry required to work up all of them antibodies and be able to troubleshoot them as well as being able to help the pathologists with their questions. For example, the antibody CEA--there is a monoclonal and a polyclonal antibody for this and the pathologist called me and asked for CEA and I asked hime if he wanted monoclonal or polyclonal and he asked me what the difference is. The person doing the IHC will have to have the knowledge to tell him that since he was ordering it on a liver biopsy and he was looking for cancer originating from the colon, what he probably wanted was CEA polyclonal, as this antibody is for liver metasasis from colonic cancers, and he probably wanted a hepar done as well. Let me know if you need any other help----be glad to offer my 2 cents any time Roxanne Soto ----- Original Message ----- From: Krat18@aol.com To: histonet@lists.utsouthwestern.edu Sent: Saturday, April 02, 2005 1:26 PM Subject: [Histonet] Who does the immunos? What policy do most labs have about letting their techs do immunos? Are all the techs trained to do them, or are only the registered techs trained to do them, or do only a limited number of techs do them? Our program is fairly new, and there is a discussion among the pathologists and administration and techs about what is the best policy. I'd like to hear from other labs about their procedures. Thanks in advance. Karen _krat18@aol.com_ (mailto:krat18@aol.com) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Sun Apr 3 14:08:04 2005 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Who does the immunos? Message-ID: Our lab is separate from the Surgical Pathology lab and is manned by two techs (an MT and an HT) that only do IHC and ISH. Periodically we've had the Surg. Path. techs rotate through to be able to back us up as the need arises but this hasn't been happening of late due to staff shortages. At the very least, we are able to have the Surg. Path. techs cut our slides as needed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Krat18@aol.com Sent: Sat 4/2/2005 12:26 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Who does the immunos? What policy do most labs have about letting their techs do immunos? Are all the techs trained to do them, or are only the registered techs trained to do them, or do only a limited number of techs do them? Our program is fairly new, and there is a discussion among the pathologists and administration and techs about what is the best policy. I'd like to hear from other labs about their procedures. Thanks in advance. Karen _krat18@aol.com_ (mailto:krat18@aol.com) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ftulenko06 <@t> jcu.edu Sun Apr 3 14:48:14 2005 From: ftulenko06 <@t> jcu.edu (ftulenko06@jcu.edu) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] question Message-ID: <803da5d8.e75c2a89.8237e00@mirapoint.jcu.edu> Hello, I have been serially sectioning turtle embryo skulls embedded in Paraplast. The ribbons are being mounted on UltraStick/Ultrafrost Adhesion slides in a hot water-bath, and the slides placed on a slide warmer. Most sections remain intact in the position they were cut, but it seems that random sections shift in position while sitting on the slide warmer. I am not sure why this is occurring. Could it be the result of water between the tissue and the slide? Any advice on how to avoid this would be appreciated. Thanks, Frank From jnocito <@t> satx.rr.com Sun Apr 3 15:26:05 2005 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Paying the high ASCP fees each year References: <200504020854.1dhLSO6fn3NZFpr0@mx-a065a01.pas.sa.earthlink.net> <000c01c52ff3$3483abd0$29d1df42@TARANGO> Message-ID: <000f01c5388b$5d19aae0$7929f318@yourxhtr8hvc4p> Mark, well said, but starting this year, whoever passes the registry has to renew their registry every three years or their certification expires. This is another thorn in my side. Yes, everyone should have continuing education, that's a fact, but to have people renew their certification every three years is just another avenue for the ASCP to collect money. Gee, they are sounding more and more like the IRS. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Mark Tarango" To: Sent: Wednesday, March 23, 2005 4:56 PM Subject: [Histonet] Paying the high ASCP fees each year > Why pay ASCP to bully me around? I pay NSH, and CSH(California) every > year because its worth it, and they don't try and force you into it. I > like reading the JOH, that alone makes the NSH dues worthwhile. ASCP, I > won't pay. They're not even friendly on the phone. Why can't the NSH > just start certifying people? > > Mark Tarango > >>Message: 23 >>Date: Sat, 2 Apr 2005 09:52:14 -0700 >>From: "Patsy Ruegg" >>Subject: [Histonet] participation >>To: "'Mark Tarango'" , >> >>Message-ID: <200504021652.j32GqscS088263@pro12.abac.com> >>Content-Type: text/plain; charset="us-ascii". > >>I must chime in here a bit. Those who do not participate in the their >>professional organizations (ASCP, NSH, state societies, etc.) are missing >>out on resources that they of course don't know anything about because >>they >>are not engaged. Even when you do pay ASCP dues each year the benefits are >>not always transparent. ASCP is the Certifying agency for our profession, >>without them none of us would have documentation that we are >>"professionals". I know that there are plenty of people out there who may >>have been working in the field for a long time and can perform the >>required >>tasks, but the fact that they have not gotten certified says something >>about >>them that frankly would not impress me as a perspective employer. With a >>few exceptions (ie Jackie O, who is certified I know) I would be willing >>to >>bet that these non-certified, non-engaged people do not read the Journals >>or >>attend classes in their field, which again would not impress me as their >>employer. >>It is our job to educate the administrators. They often have no idea that >>non-certified people are working for them or even that certification is >>available in some cases. NSH has been working for many years to try and >>get >>CAP to require certified Histotech's be working in the histo lab, we even >>asked that they at least require supervisors be certified. CAP still has >>no >>such requirements. It can be a long tuff battle, but we are making >>progress. ASCP, NSH, CAP and State Histology Societies, to mention a few, >>are our professional bargaining agencies. Through them we will make the >>changes in the field we want to see. Without these agencies we are just >>individuals whining about issues we are doing nothing to affect. > >>Best regards, > >>Patsy Ruegg > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Anti-Virus. > Version: 7.0.308 / Virus Database: 266.9.1 - Release Date: 4/1/2005 > > From jnocito <@t> satx.rr.com Sun Apr 3 15:29:12 2005 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Who does the immunos? References: Message-ID: <001601c5388b$cc8c3f00$7929f318@yourxhtr8hvc4p> Karen, my primary immuno tech has 20 years or Histo experience and her HT. Her back up has a BS, 20 years of experience. The reason why it is like this is that I hired one tech who I taught immunos to, the tech with a degree has no immuno experience at all ad wants to learn. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: To: Sent: Saturday, April 02, 2005 1:26 PM Subject: [Histonet] Who does the immunos? > What policy do most labs have about letting their techs do immunos? Are > all the techs trained to do them, or are only the registered techs > trained to > do them, or do only a limited number of techs do them? Our program is > fairly > new, and there is a discussion among the pathologists and administration > and > techs about what is the best policy. I'd like to hear from other labs > about their procedures. > > Thanks in advance. > > Karen > _krat18@aol.com_ (mailto:krat18@aol.com) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Anti-Virus. > Version: 7.0.308 / Virus Database: 266.9.1 - Release Date: 4/1/2005 > > From hawkmoon15 <@t> cox.net Sun Apr 3 16:30:13 2005 From: hawkmoon15 <@t> cox.net (Sarah Jones) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Paying the high ASCP fees each year References: <200504020854.1dhLSO6fn3NZFpr0@mx-a065a01.pas.sa.earthlink.net><000c01c52ff3$3483abd0$29d1df42@TARANGO> <000f01c5388b$5d19aae0$7929f318@yourxhtr8hvc4p> Message-ID: <001401c53894$523d6e00$9756e344@sd.cox.net> Okay, okay....I have had to bite my lip to stay out of this until now. However, I can no longer stand by and see this crusifiction continue for the ASCP. A few years back, the "American Society of Clinical Pathologists" became the "American Society of Clinical Pathology". There was a HUGE HUSH when this was done. To the best of my knowledge, this was not done with any kind of fanfare. TRUST ME! We, as a professional group, are MUCH better off now than we were in the many, many years prior, during which time our dues stayed at the level (far cheaper) amount. I go waaaaaaaaaayyyyyyyyyyyyy back, to when the Registry (ASCP) was in Muncie, Indiana....this was prior to the move to Chicago, Illinois. When the ASCP was basically 'owned' by the Pathologists, many things were different. Our dues remained the same for EONS. In fact, I wondered why they never seemed to change. However, our salaries were as etched in stone as the dues were. THAT, my friends, was because the Pathologists did not allow for any significant salary increases, which was what they were (IMHO) trying to accomplish. In other words, if you were 'ASCP', they could then control your wages. Of course, that was back in the days before we were in such a short supply. Soooooooo....my best guess is that the Pathologists let go of any control AND financial support they had on the ASCP when it became obvious that we ARE in such short supply. I am sure that when that happened, the ASCP had to start raising the fees to simply survive. The ASCP was there for us LONG before the NSH. I belong to the NSH as well, and I realize all they have done for us. In addition, I belong to our state Histology Society. However, I have no intention of discarding the ASCP now, simply because they are now in need of re-defining their support and what they can offer each dicipline in continuing education. I can remember being in Chicago for the meeting from which the NSH arose. That was done because we Histotechs felt that we needed better representation than we had to that point in time. Yes, the NSH does more for us as an individual group than the ASCP has ever done. However, you have to consider that the ASCP represents far more disciplines than ours! In addition, the ASCP was there prior to any other. Surely THAT means something to you! Sincerely, Sarah A. Jones sarah.jones@dakocytomation.com ----- Original Message ----- From: "Joe Nocito" To: "Mark Tarango" ; Sent: Sunday, April 03, 2005 1:26 PM Subject: Re: [Histonet] Paying the high ASCP fees each year > Mark, > well said, but starting this year, whoever passes the registry has to renew > their registry every three years or their certification expires. This is > another thorn in my side. > Yes, everyone should have continuing education, that's a fact, but to > have people renew their certification every three years is just another > avenue for the ASCP to collect money. Gee, they are sounding more and more > like the IRS. > > Joe Nocito BS, HT(ASCP)QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > ----- Original Message ----- > From: "Mark Tarango" > To: > Sent: Wednesday, March 23, 2005 4:56 PM > Subject: [Histonet] Paying the high ASCP fees each year > > > > Why pay ASCP to bully me around? I pay NSH, and CSH(California) every > > year because its worth it, and they don't try and force you into it. I > > like reading the JOH, that alone makes the NSH dues worthwhile. ASCP, I > > won't pay. They're not even friendly on the phone. Why can't the NSH > > just start certifying people? > > > > Mark Tarango > > > >>Message: 23 > >>Date: Sat, 2 Apr 2005 09:52:14 -0700 > >>From: "Patsy Ruegg" > >>Subject: [Histonet] participation > >>To: "'Mark Tarango'" , > >> > >>Message-ID: <200504021652.j32GqscS088263@pro12.abac.com> > >>Content-Type: text/plain; charset="us-ascii". > > > >>I must chime in here a bit. Those who do not participate in the their > >>professional organizations (ASCP, NSH, state societies, etc.) are missing > >>out on resources that they of course don't know anything about because > >>they > >>are not engaged. Even when you do pay ASCP dues each year the benefits are > >>not always transparent. ASCP is the Certifying agency for our profession, > >>without them none of us would have documentation that we are > >>"professionals". I know that there are plenty of people out there who may > >>have been working in the field for a long time and can perform the > >>required > >>tasks, but the fact that they have not gotten certified says something > >>about > >>them that frankly would not impress me as a perspective employer. With a > >>few exceptions (ie Jackie O, who is certified I know) I would be willing > >>to > >>bet that these non-certified, non-engaged people do not read the Journals > >>or > >>attend classes in their field, which again would not impress me as their > >>employer. > >>It is our job to educate the administrators. They often have no idea that > >>non-certified people are working for them or even that certification is > >>available in some cases. NSH has been working for many years to try and > >>get > >>CAP to require certified Histotech's be working in the histo lab, we even > >>asked that they at least require supervisors be certified. CAP still has > >>no > >>such requirements. It can be a long tuff battle, but we are making > >>progress. ASCP, NSH, CAP and State Histology Societies, to mention a few, > >>are our professional bargaining agencies. Through them we will make the > >>changes in the field we want to see. Without these agencies we are just > >>individuals whining about issues we are doing nothing to affect. > > > >>Best regards, > > > >>Patsy Ruegg > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > No virus found in this incoming message. > > Checked by AVG Anti-Virus. > > Version: 7.0.308 / Virus Database: 266.9.1 - Release Date: 4/1/2005 > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rod.coombe <@t> imvs.sa.gov.au Sun Apr 3 19:32:23 2005 From: rod.coombe <@t> imvs.sa.gov.au (Rod Coombe) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Cassette and slide microwriters Message-ID: <000601c538ad$c516ca70$d86d140a@ITP36079> Hello all, I would very much appreciate feedback, good and bad on cassette and slide microwiters. Which ones are best and what success people have had interfacing them to their laboratory information system, especially Ultra (Triple G). Thankyou Rod Coombe Manager Tissue Pathology IMVS Frome Road, Adelaide South Australia Phone (08) 82223210 / 0401120808 From JWEEMS <@t> sjha.org Sun Apr 3 19:44:53 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Cassette and slide microwriters Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA9D267@sjhaexc02.sjha.org> Please be sure to reply to the net. I am trying to get Sakura set up with Triple G right now. It is a slow process. Thanks! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rod Coombe Sent: Sun 4/3/2005 8:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette and slide microwriters Hello all, I would very much appreciate feedback, good and bad on cassette and slide microwiters. Which ones are best and what success people have had interfacing them to their laboratory information system, especially Ultra (Triple G). Thankyou Rod Coombe Manager Tissue Pathology IMVS Frome Road, Adelaide South Australia Phone (08) 82223210 / 0401120808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From jessgrocki <@t> yahoo.com Sun Apr 3 19:49:14 2005 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Job at UConn Health Center Message-ID: <20050404004914.77462.qmail@web81709.mail.yahoo.com> Hi Everyone, Just wanted to let everyone know that there is a FT histotechnologist position at UConn Health Center in Connecticut. If you go to www.uchc.edu you can read the full description. No one posted it so I thought I'd let everyone know in case someone is interested. I am a former employee so if anyone wants to know what its like to work there I can give you a heads up. Good Luck, Jessica Piche-Grocki, HT (ASCP) From godsgirlnow <@t> MSN.COM Sun Apr 3 19:58:00 2005 From: godsgirlnow <@t> MSN.COM (Roxanne Soto) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Who does the immunos? 2-cents more References: <20050403145441.85631.qmail@web81403.mail.yahoo.com> Message-ID: This is why many are requiring that those that do IHC have their qualification in IHC, because it requires continuing education. Also, job descriptions for techs that do IHC should have some kind of continuing education requirement in it. Roxanne ----- Original Message ----- From: Georgia Stewart To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 03, 2005 10:54 AM Subject: Re: [Histonet] Who does the immunos? 2-cents more Roxanne, What you said is very true, but unfortunately in the laboratory where I worked the pathologists didn't function in this way. The supervisor is certified HT but her only continuing education has been the 5 days she spent at Ventana learing how to use their automated IHC stainer. California does not license HTs or HTLs and as far as I know there is no requirement that a tech be certified before they can perform IHC in California. One lab I'm familiar with, only allows the med techs to perform the IHC (on an automated stainer) because they are "educated and licensed" and don't allow the HTs to perform any IHC. Don't think anyone at this facility is HTL certified. ASCP is some of the problem, but I feel it is also, in part, a result of the pathologists not wanting to recognize the ongoing need for continuing education, and not just in IHC. The lab offered no incentive for continuing education, either in monetary assistance to attend seminars/state histology meetings, etc., or any increase in salary because of continuing education paid for by the tech. The only ones who received monetary assistance were the cytotechs who are required to have continuing education. The cytotechs are licensed by the state of California and they are also ASCP certified. In this lab, licensing was the motivating factor for the pathologists to pay for the cytotechs continuing education. Georgia Roxanne Soto > wrote: Karen, This would depend on your state and you institution. Many institutions only allow technologists to do them. Some states will aonly allow Technologists to do them. If this is a brand new job description, then it can be incorporated into the job description. If it is an existng description, review it and see what it says, you can always add it to the current job description. I would recommend that it be a separate job description for the IHC Tech, because it takes a special kind of tech to be able to handle all of the stress and math and chemistry required to work up all of them antibodies and be able to troubleshoot them as well as being able to help the pathologists with their questions. For example, the antibody CEA--there is a monoclonal and a polyclonal antibody for this and the pathologist called me and asked for CEA and I asked hime if he wanted monoclonal or polyclonal and he asked me what the difference is. The person doing the IHC will have to have the knowledge to tell him that since he was ordering it on a liver biopsy and he was looking for cancer originating from the colon, what he probably wanted was CEA polyclonal, as this antibody is for liver metasasis from colonic cancers, and he probably wanted a hepar done as well. Let me know if you need any other help----be glad to offer my 2 cents any time Roxanne Soto ----- Original Message ----- From: Krat18@aol.com To: histonet@lists.utsouthwestern.edu Sent: Saturday, April 02, 2005 1:26 PM Subject: [Histonet] Who does the immunos? What policy do most labs have about letting their techs do immunos? Are all the techs trained to do them, or are only the registered techs trained to do them, or do only a limited number of techs do them? Our program is fairly new, and there is a discussion among the pathologists and administration and techs about what is the best policy. I'd like to hear from other labs about their procedures. Thanks in advance. Karen _krat18@aol.com_ (mailto:krat18@aol.com) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Megan.Clarke <@t> hnehealth.nsw.gov.au Sun Apr 3 21:54:35 2005 From: Megan.Clarke <@t> hnehealth.nsw.gov.au (Megan Clarke) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] BAF47 Message-ID: Hi Histonetters, Would like your help.We are trying to find an antibody BAF47/SNF5-anti-INI1 Can anyone please help us in the search for this. We would like it for formalin fixed parrafin embedded sections. Thank you for your help Megan Clarke Immunohistochemistry Lab HAPS,Newcastle AUSTRALIA From euan.mcnaughtone <@t> agresearch.co.nz Sun Apr 3 23:03:21 2005 From: euan.mcnaughtone <@t> agresearch.co.nz (McNaughton, Euan) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Secondary antibody-HRP conjugates Message-ID: Hi All, Has anyone had any experience with Zymed SuperPicTure or other kits in which HRP is directly conjugated to the secondary antibody? I can see why it'd be useful for tissues that have high levels of endogenous biotin, but I'm curious about their claims of "superior sensitivity" for general use. I'm quite new to the IHC game, but I thought that the avidin-biotin system was used to improve senstivity, and so removing it would have the opposite effect... Any comments would be appreciated! Cheers, Euan McNaughton ======================================================================= Attention: The information contained in this message and/or attachments from AgResearch Limited is intended only for the persons or entities to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipients is prohibited by AgResearch Limited. If you have received this message in error, please notify the sender immediately. ======================================================================= From sverma8 <@t> lycos.com Mon Apr 4 04:44:15 2005 From: sverma8 <@t> lycos.com (Subhash Verma) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Gayle Callis-PNA staining Message-ID: <20050404094415.DC5A1CA099@ws7-4.us4.outblaze.com> Hi Gayle, I have been unsuccessful to stain germinal centres from bovine spleen and lymphnodes (formalin fixed parafin embedded sections)with lectin conjuagated to HRP as per the protocol suggetsed by you. Any suggestions, please. Thanks. SV -- _______________________________________________ NEW! Lycos Dating Search. The only place to search multiple dating sites at once. http://datingsearch.lycos.com From mtyler <@t> uctgsh1.uct.ac.za Mon Apr 4 05:06:16 2005 From: mtyler <@t> uctgsh1.uct.ac.za (Tyler) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Frozen sections Message-ID: <42511198.29D397E2@uctgsh1.uct.ac.za> Hi Asking this question for a colleague who does the flourescent staining on frozen sections.Does it make a difference to fluorescent staining if one fixes cryosections in acetone before freezing the slides at -80?C? Is it better to rather store the sections unfixed before freezing? Thank you Marilyn From mtyler <@t> uctgsh1.uct.ac.za Mon Apr 4 05:35:28 2005 From: mtyler <@t> uctgsh1.uct.ac.za (Tyler) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] [Fwd: Frozen sections] Message-ID: <42511870.E7EEFF3@uctgsh1.uct.ac.za> From GDawson <@t> dynacaremilwaukee.com Mon Apr 4 07:27:34 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] QIHC Message-ID: Hi, everybody. I'm wondering if anyone who has recently taken the new QIHC computer exam can recommend good study material - I'm feeleng pretty apprehensive. Thank you! Aurora Kallenbach, HT(ASCP) Dynacare Laboratories, Milwaukee, WI histoaurora@yahoo.com From kelly.mcqueeney <@t> bms.com Mon Apr 4 08:12:33 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: ASCP - My 2 cents In-Reply-To: References: Message-ID: <42513D41.6040808@bms.com> But lets say I wanted to be a Histotechnologist (is that the term?), what kind of education would I need? What is the difference in education for a Pathology Assistant, Technologist, etc? Sorry for the simple question, I read everyone's emails about certification and I'm just curious. Very interesting.... Kelly McQueeney Robyn Vazquez wrote: >Kelly, > >I have been doing histology for going on 15 years (?) and never certified as my own choice. I do EXCELLENT QUALITY work. I was taught in the military as OTJ training. > >Robyn > > > > From gliuygao <@t> hotmail.com Mon Apr 4 08:19:20 2005 From: gliuygao <@t> hotmail.com (yan gao) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] ERk antibody Message-ID: >From: "yan gao" >To: histonet@pathology.swmed.edu >Subject: [Histonet] ERk antibody >Date: Fri, 01 Apr 2005 14:54:16 -0500 > > > >From: "yan gao" > >To: histonet@lists.utsouthwestern.edu, > histonet@list.utsouthwestern.edu > >Subject: [Histonet] (no subject) > >Date: Fri, 01 Apr 2005 14:50:34 -0500 > > > > > > > > Dear Histonet, > > > > I had some trouble stain pERK antibody and monoclone pMEK > >antibody on > > xenograft melanoma tumor. Can anyone share some tips for me. >I > >did try > > cell signaling technology #4376 & 3910. I see weak stain on > >#4376. Thanks. > > > > > > Best Regards, > > > > Yan > > > > Novartis Institute for BioMedical Research > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Mon Apr 4 08:21:22 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: ASCP - My 2 cents In-Reply-To: Message-ID: Chuck, here in Texas, the pathologist's lobbyist shot down a licensure requirement for Texas. I guess they thought that they might have to pay for CE or worse, increase the salary base. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Friday, April 01, 2005 4:07 PM To: Rittman, Barry R; histonet Subject: RE: [Histonet] Re: ASCP - My 2 cents Barry, You hit the nail right on the head: "If expertise and salaries are tied together and for all defined positions there is a requirement (for HIPPA or similar regulations) that in order to perform certain tests you have certification, then salary should follow." Regulations you speak of are more like CLIA '88, CAP guidelines and licensure. Except for a few states, like Florida, anyone can fully function as a histotech without any type of certification. Many states are now addressing the licensure issue. The lab professions in Illinois (my state) are pushing for legislation but the histology professionals in the state requested exemption from the bill because they were afraid of a negative impact. Licensure is great for those that are able to be licensed (certified) but can be a nightmare for those that have chosen to work in the field for many years (I think Robyn says she has been working 15) without certification that now suddenly find themselves legislated out of a job. Pathologists and lab managers also feel the pinch in having to search for certified people and the heartache of having to terminate loyal employees because they don't meet the licensing requirements. In the 25 years I have been in the field I have seen pay and benefits increase more so on the upper limits but not so much in the starting range. Licensure can be a double edge sword. We just have to remember to be prepared and duck when we see it coming. Charles Embrey PA(ASCP), HT(ASCP) Urbana IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Friday, April 01, 2005 3:18 PM To: histonet Subject: RE: [Histonet] Re: ASCP - My 2 cents Ruth I can see where you are coming from but I do not agree with this concept. It is fine for individual histotechs to try to get as much salary as possible from their current employer, good luck to y'all for this. However this does not change the overall situation for histotechs in general and depends on a one on one interaction or confrontation. The concept of the squeaky wheel springs to mind. What is desperately needed is a uniform salary base for histotechs depending on their level, certification etc. This does not mean that salaries would have to be the same for all regardless of their location, but there would be a baseline for all that all employers would have to abide by. We all recognize that some places are simply more expensive to live at than others. What is needed is a base scale with appropriate adjustments for location and for each stage of advancement in the profession. If expertise and salaries are tied together and for all defined positions there is a requirement (for HIPPA or similar regulations) that in order to perform certain tests you have certification, then salary should follow. A united approach is, I believe, the only way to ensure our profession maintains its standards of excellence and also is recognized for its contributions to the health of patients. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cazares, Ruth Sent: Friday, April 01, 2005 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: ASCP - My 2 cents Hi all, I just have to add my opinion to what is going on, on the Histo net. Don't department heads have a say in what the qualifications should be for tech positions? Maybe it is time to get together with the powers that be and make a change. ASCP has raised the education level for certifying histo-techs, this should be an opportunity to get together with your administrators and make requirement changes for your Histo tech positions. That is exactly what I am going to do. If your institution prefers to hire uncertified people to do the work certified techs should be doing, is this a place you really want to work? Everyone deserves to be treated with respect and deserves to know that their hard work is appreciated. If you don't get this where you are at, leave. There are a lot of other places in need of histo-techs. After my job was phased out at a hospital where I had worked for 10 years, I went to work at 3 different labs before I found my niche. We spend too much of our daily lives at our jobs, we don't need to be unhappy and with high blood pressure the minute we walk into our lab. Life is too short. And as far as Continuing Education, NSH offers online CE and teleconferences (about 8 or 9 a year ). Leica and Thermoshandon (is that their name now?) as I'm sure other vendors do also, offer 1 day workshops for CEU's . The continuing education for our field is out there, sometimes we just have to look for it. P.S. I'll have a position open in December, anybody interested? Lots of Love to all my Histo brothers and sisters, Ruth Cazares HT (ASCP) Department of Pathology Swedish Covenant Hospital Chicago, IL 60625 *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tflore <@t> lsuhsc.edu Mon Apr 4 08:29:23 2005 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] microns on frozen sections of lung Message-ID: Is there an "ideal temperature" to frozen section lung tissue? Any input would surely be appreciated, as I usually frozen section, at 4-6 microns, renal, skin and heart between -15 and -12 o C degrees, with not a lot of problem. But this last time, lung tissue was included and I had such great difficulty in obtaining a decent frozen section and was almost embarrassed to turn in what I was able to obtain. Teresa Flores LSUHSC EM Lab New Orleans, LA From Charles.Embrey <@t> carle.com Mon Apr 4 08:39:50 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: A Histotechnologist requires: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnologist or Histologic Technician program. Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry, AND one year full time acceptable experience in a histopathology laboratory within the last ten years. This year of experience must be under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology, or eligible) or an appropriately certified medical scientist. Histotechnician Requires: Route 1: Successful completion of a NAACLS accredited Histotechnician program. Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology, or eligible), or an appropriately certified medical scientist. And the Pathologists' Assistant requires: Route 1: Baccalaureate degree from a regionally accredited college/university AND successful completion of a NAACLS accredited Pathologists' Assistant program within the last five years, OR Route 2: Baccalaureate degree from a regionally accredited college/university with 20 semester hours (30 quarter hours) of biology, AND three years full time acceptable experience as a Pathologists' Assistant within the last ten years. The three years of experience must be under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology). NOTE: Route 2 will expire effective December 31, 2007. After that date, all candidates will be required to complete a NAACLS accredited Pathologists' Assistant Program. Please also note that all but one (Wayne State) Pathologists' Assistant programs are Master Level programs. Charles Embrey PA(ASCP) Urbana IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly D Mcqueeney Sent: Monday, April 04, 2005 8:13 AM To: Robyn Vazquez Cc: histonet@lists.utsouthwestern.edu; RCazares@schosp.org Subject: Re: [Histonet] Re: ASCP - My 2 cents But lets say I wanted to be a Histotechnologist (is that the term?), what kind of education would I need? What is the difference in education for a Pathology Assistant, Technologist, etc? Sorry for the simple question, I read everyone's emails about certification and I'm just curious. Very interesting.... Kelly McQueeney Robyn Vazquez wrote: >Kelly, > >I have been doing histology for going on 15 years (?) and never certified as my own choice. I do EXCELLENT QUALITY work. I was taught in the military as OTJ training. > >Robyn > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tflore <@t> lsuhsc.edu Mon Apr 4 08:34:45 2005 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Technical Fees for frozen sections, anyone? Message-ID: A request of frozen sectioning heart and lung tissue on a research project has been asked by a fellow doing this research. He would like to know what our lab would charge for "just frozen sectioning a couple of slides" I, of course, informed the fellow that it would be $50.00 a slide, as material is brought in over embedded in OCT and has to be trimmed down to fit the molds used in the cryostat; the material is also brought unevenly embedded; and there is always a "stat" deadline to meet due to a grant being submitted. Has anyone else had to do this type of work and is willing to share what they charged? Thanking in advance to responses, Teresa Flores LSUHSC EM Lab New Orleans, LA From ian.montgomery <@t> bio.gla.ac.uk Mon Apr 4 08:54:01 2005 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:24:53 2005 Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents Message-ID: <6.2.1.2.2.20050404142705.04704dd0@udcf.gla.ac.uk> Reading these and similar postings over the last few weeks I'm truly staggered that a number of my colleagues in the US have an apparent disregard for academic qualifications. While OJT is invaluable, without decent recognised qualifications they are as nothing. How on earth do you think our jobs will be recognised as a distinct specialised branch of science without formal qualifications. Get a grip of yourselves, the more highly qualified we become the greater our standing in the scientific community. Educationally, I have a first and second degree but that is not enough to make me a fully rounded scientist so I have started another degree course and I'm in the twilight of my career. Please, education is there, grasp it, it's a precious beautiful gift, don't let our branch of science become some OJT training only job, we are career scientists, never forget it. Take home message from my rant, get qualified as soon as possible. Any qualification in biological science, coupled with suitable training makes you a more attractive proposition to a prospective employer. You can demonstrate both technical ability and that you have an open enquiring mind receptive to the ever changing world of biological science. Ian. >But lets say I wanted to be a Histotechnologist (is that the term?), what >kind of education would I need? What is the difference in education for a >Pathology Assistant, Technologist, etc? Sorry for the simple question, I >read everyone's emails about certification and I'm just curious. Very >interesting.... > >Kelly McQueeney > > >Robyn Vazquez wrote: > >>Kelly, >> >>I have been doing histology for going on 15 years (?) and never certified >>as my own choice. I do EXCELLENT QUALITY work. I was taught in the >>military as OTJ training. >> >>Robyn >> >> > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07623 975451 e-mail: ian.montgomery@bio.gla.ac.uk From Charles.Embrey <@t> carle.com Mon Apr 4 09:09:01 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: Larry, You are partially right. If a state begins to license a trade they normally do give a few years for those working to fulfill the requirements. It really depends on how the law is written as to what happens next. The state may just "grandfather" in anyone currently working in the field or (most probably) require them to pass the licensure test. This is were histotechs can get burned. If the state follows Florida's lead and counts the ASCP/BOR as the licensing test for the State but the practicing HT can't be certified by ASCP because they don't meet the new education standards they would be legislated out of their job. It's a cruel fact but very possible. Pathologists normally fight the licensure issue because they don't want the State dictating who they have to hire. In my experience I have found that licensure doesn't always equal quality Charles Embrey -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: Friday, April 01, 2005 4:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: ASCP - My 2 cents Well get out the violins I'm tearing up here. Why would you choose not to get certified if you "love" your job so much. If you've been in the field that long it would be a snap to get certified and besides they wouldn't be firing anyone if they made licensure mandatory, it would have to begin at a future date, everyone else would be grandfathered in. As far as pathologists not being able to find qualified people, they are partly to blame, they don't want mandatory licensure. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sue.Kapoor <@t> uhsi.org Mon Apr 4 09:16:26 2005 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] FW: Need used hood for Tissue-Tek DRS-601 stainer Message-ID: <61E9F2400F53D5119CFC00508B44E33B02E9E0BE@khmcexch.uhsi.org> -----Original Message----- From: Kapoor, Sue Sent: Monday, March 28, 2005 2:50 PM To: histonet@pathology.swmed.edu Subject: Need used hood for Tissue-Tek DRS-601 stainer To all Vendors - I'm looking for a used/refurbished hood for this stainer. Please email off line with quotes to: Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 Thank you! From abright <@t> brightinstruments.com Mon Apr 4 09:30:44 2005 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] microns on frozen sections of lung Message-ID: Teresa, For frozen sections of lung -18 to -20? C, specimen temperature. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com -----Original Message----- From: Flores, Teresa [mailto:tflore@lsuhsc.edu] Sent: 04 April 2005 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microns on frozen sections of lung Is there an "ideal temperature" to frozen section lung tissue? Any input would surely be appreciated, as I usually frozen section, at 4-6 microns, renal, skin and heart between -15 and -12 o C degrees, with not a lot of problem. But this last time, lung tissue was included and I had such great difficulty in obtaining a decent frozen section and was almost embarrassed to turn in what I was able to obtain. Teresa Flores LSUHSC EM Lab New Orleans, LA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfockler <@t> mail1.vcu.edu Mon Apr 4 09:39:33 2005 From: cfockler <@t> mail1.vcu.edu (cfockler@mail1.vcu.edu) Date: Fri Sep 16 15:24:53 2005 Subject: [Histonet] Re: ASCP - My 2 cents In-Reply-To: <42513D41.6040808@bms.com> Message-ID: <200504041439.KAA08840@arrakis.vcu.edu> I have listened to all of this talk about certification, and no one has yet to comment on those of us with degrees. Should a histotech with a degree start at a higher salary base? What about certification? Considering we are only eligible after a year in the field, we are a lot of times neglected as far as base salary is concerned even though we do have a degree, but are not yet certified. How should ASCP, or even our employers address that issue? Candyce Fockler Histotechnologist VCUHS ------------------- > But lets say I wanted to be a Histotechnologist (is that the term?), > what kind of education would I need? What is the difference in education > for a Pathology Assistant, Technologist, etc? Sorry for the simple > question, I read everyone's emails about certification and I'm just > curious. Very interesting.... > > Kelly McQueeney > > > Robyn Vazquez wrote: > > >Kelly, > > > >I have been doing histology for going on 15 years (?) and never certified as my own choice. I do EXCELLENT QUALITY work. I was taught in the military as OTJ training. > > > >Robyn > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From c.m.vanderloos <@t> amc.uva.nl Mon Apr 4 09:46:20 2005 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] RE: Frozen sections Message-ID: <3364f86335ffe5.335ffe53364f86@amc.uva.nl>

Hi,

Good question!

Actually we never tried to fix&n acetone before storage at -80C. The only t know is that proper storage at -80C of unfixed cryostat section works well for a long time. Storing them unfixed gives you at least t example, antigens preferabl much better in this res face=Times size=2>Chris van der Loos, PhD
D Pathology
Academical Medical Center M2-230
Meiber gdreef 9
NL-1105 AZ Amsterdam
The Netherlands< size=2>phone:  +31 20 5665631< BR>fax:    +31 20 6960389
e-mail: href="mailto:c.m.vanderloos@amc.uva.nl" face=Times size=2>c.m.vanderloos@amc.uva.nl< /FONT>

 

----- Original Message -----

From  Tyler <mtyler@uctgsh1. uct.ac.za>
Date  Mon, 04 Apr 2005 12:06:16 + 0200
To  histonet <histonet@lists .utsouthwestern.edu>
Subject&nb [Histonet] Frozen sections<

Hi
Asking this question for sections. if
one fixes cry slides at -80?C?
Is i sections unfixed before freezing? Thank< BR>you
Marilyn

From Dixon.Leslie <@t> mayo.edu Mon Apr 4 09:38:45 2005 From: Dixon.Leslie <@t> mayo.edu (Dixon, Leslie E.) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: <115ED45FBA6D3D4B89E7F40A9EB5339D0361C1EC@excsrv60.mayo.edu> To continue on Candyce's thought. What about those with HTL certification? I have never had an employer differentiate between HT and HTL despite the difference in education and expected knowledge. Leslie -----Original Message----- From: cfockler@mail1.vcu.edu [mailto:cfockler@mail1.vcu.edu] Sent: Monday, April 04, 2005 7:40 AM To: Kelly D Mcqueeney Cc: histonet@lists.utsouthwestern.edu; RCazares@schosp.org Subject: Re: [Histonet] Re: ASCP - My 2 cents I have listened to all of this talk about certification, and no one has yet to comment on those of us with degrees. Should a histotech with a degree start at a higher salary base? What about certification? Considering we are only eligible after a year in the field, we are a lot of times neglected as far as base salary is concerned even though we do have a degree, but are not yet certified. How should ASCP, or even our employers address that issue? Candyce Fockler Histotechnologist VCUHS ------------------- > But lets say I wanted to be a Histotechnologist (is that the term?), > what kind of education would I need? What is the difference in education > for a Pathology Assistant, Technologist, etc? Sorry for the simple > question, I read everyone's emails about certification and I'm just > curious. Very interesting.... > > Kelly McQueeney > > > Robyn Vazquez wrote: > > >Kelly, > > > >I have been doing histology for going on 15 years (?) and never certified as my own choice. I do EXCELLENT QUALITY work. I was taught in the military as OTJ training. > > > >Robyn > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Mon Apr 4 09:51:37 2005 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] ASCP - my 2 cents Message-ID: <20050404145138.84370.qmail@web50308.mail.yahoo.com> Bravo Ian. I could not agree more. I went through an accredited 2 year histology program, got an Associates, and then received my HT. Some years later they began the HTL. I went back to school (while having small children) to get my Bachelors degree so I could take it and I passed with flying colors. I feel this field requires you have more than just chemistry and biology 101 if you want to fully understand HOW everything works and what to do when it doesn't. I still search the net and read all I can to keep up when something new comes along. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From slappycraw <@t> yahoo.com Mon Apr 4 10:02:50 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:54 2005 Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents Message-ID: <20050404150250.16287.qmail@web52609.mail.yahoo.com> Bravo Ian: The problem here in the US is that there are thousands upon thousands of people working in histology labs that are not registered and don't have the theory behind histology and probably never will, and that is just the way some institutions and organizations want to keep it. That is not to say that some of these people are not dedicated to their jobs but if your in the field why not get the registry. Larry --------------------------------- Do you Yahoo!? Take Yahoo! Mail with you! Get it on your mobile phone. From gcallis <@t> montana.edu Mon Apr 4 10:05:40 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Contacting Re: Linda Durbin Exackt In-Reply-To: <1112386922.424dad6a2d7f9@imp.vet.upenn.edu> References: <1112386922.424dad6a2d7f9@imp.vet.upenn.edu> Message-ID: <6.0.0.22.1.20050404090412.01b6caf0@gemini.msu.montana.edu> Linda Durbin At 02:22 PM 4/1/2005, you wrote: >Hi All, > > >Sorry to bother the list with this however, I am attempting to reach Linda >Durbin. > >Linda if you see this or someone lets you know please call Pam Marcum at >610-925-6278. >I am at the University of PA Vet School and need to speak with you. > >Pam Marcum > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From kelly.mcqueeney <@t> bms.com Mon Apr 4 10:11:55 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:54 2005 Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents In-Reply-To: <6.2.1.2.2.20050404142705.04704dd0@udcf.gla.ac.uk> References: <6.2.1.2.2.20050404142705.04704dd0@udcf.gla.ac.uk> Message-ID: <4251593B.3070204@bms.com> Thanks for the pep talk, Ian. I have been interested in hearing opinions of people in your field. I don't work in a clinical lab, I'm just along for the histonet ride in case I need advice and expertise in histology. Truly, the advice I have received is phenominal and I consider the all histonetters experts when it comes to histology. I have 2 degrees and it suits me just fine. I worked very hard educating myself. Believe it or not, I'm a very well rounded scientist and very qualified. I work for big pharma and histology is a only small part of our work (even though I have been doing it for 10 years). In addition, we perform studies that include all aspects of molecular biology, immunology, histology, toxicology, pharmacology, plus in vivo and in vitro analysis of multiple species. The list goes on and on... There is no better formal training than on-the-job. Employers loves seeing formal training, but they really love seeing that you dipped your hand in everything and can (or have) master many fields. It's nice to continue your education throughout your career, but what about the people who have families and second jobs? Kids in college? Family members/parents that need support? They may not have time to get all of the formal qualifications and will rely on skills they learn the field.....and better themselves everyday by being inquisitive, demanding, and ambitious. This is the best kind of education. Kelly Ian Montgomery wrote: > Reading these and similar postings over the last few weeks I'm > truly staggered that a number of my colleagues in the US have an > apparent disregard for academic qualifications. While OJT is > invaluable, without decent recognised qualifications they are as > nothing. How on earth do you think our jobs will be recognised as a > distinct specialised branch of science without formal qualifications. > Get a grip of yourselves, the more highly qualified we become the > greater our standing in the scientific community. Educationally, I > have a first and second degree but that is not enough to make me a > fully rounded scientist so I have started another degree course and > I'm in the twilight of my career. Please, education is there, grasp > it, it's a precious beautiful gift, don't let our branch of science > become some OJT training only job, we are career scientists, never > forget it. > Take home message from my rant, get qualified as soon as > possible. Any qualification in biological science, coupled with > suitable training makes you a more attractive proposition to a > prospective employer. You can demonstrate both technical ability and > that you have an open enquiring mind receptive to the ever changing > world of biological science. > Ian. > > > >> But lets say I wanted to be a Histotechnologist (is that the term?), >> what kind of education would I need? What is the difference in >> education for a Pathology Assistant, Technologist, etc? Sorry for the >> simple question, I read everyone's emails about certification and I'm >> just curious. Very interesting.... >> >> Kelly McQueeney >> >> >> Robyn Vazquez wrote: >> >>> Kelly, >>> >>> I have been doing histology for going on 15 years (?) and never >>> certified as my own choice. I do EXCELLENT QUALITY work. I was >>> taught in the military as OTJ training. >>> >>> Robyn >>> >>> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Dr. Ian Montgomery, > Histotechnology, > Graham Kerr Building, > Institute of Biomedical & Life Sciences, > University of Glasgow, > Glasgow, > G12 8QQ. > Tel: 0141 339 8855 > Office: 4652 > Lab: 6644. > Pager: 07623 975451 > e-mail: ian.montgomery@bio.gla.ac.uk > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lchung <@t> ppmh.org Mon Apr 4 10:20:58 2005 From: lchung <@t> ppmh.org (Chung, Luong) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: You wouldn't go to see an OJT Doctors or nurses would you? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry Woody Sent: Monday, April 04, 2005 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents Bravo Ian: The problem here in the US is that there are thousands upon thousands of people working in histology labs that are not registered and don't have the theory behind histology and probably never will, and that is just the way some institutions and organizations want to keep it. That is not to say that some of these people are not dedicated to their jobs but if your in the field why not get the registry. Larry --------------------------------- Do you Yahoo!? Take Yahoo! Mail with you! Get it on your mobile phone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Mon Apr 4 10:29:04 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] re: microns on frozen sections of lung Message-ID: <20050404152904.35245.qmail@web30415.mail.mud.yahoo.com> Hi Teresa, Lung tends to be a bit on the watery side. If there is pulmonaty edema it will be very watery and suseptable to freeze artefact which can make it seem like its full of holes. The more water a tissue has the warmer I cut them. I will often start trimming at at temp that is just starts to cut in a flat sheet without crumpling. As the block continues to cool on the chuck the tissue comes to the temp where it starts to cut just right. With my system we have small steel blocks that you can press to the face of the block cool it further it in small incerments if needed ( without freezing it too hard with a spray) Make sure your crostat is set up properly ( blade angle ect) and above all start with a sharp blade. If you want to make sure your problems are do to the tissue and not something wrong with the cryostat, make a block of only OCT and try cutting it. It should cut very easily. Stephen From pex0220 <@t> yahoo.com.cn Mon Apr 4 10:51:16 2005 From: pex0220 <@t> yahoo.com.cn (=?gb2312?q?=CC=EC=20=D0=C1?=) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Tween 20 Message-ID: <20050404155116.14658.qmail@web15509.mail.cnb.yahoo.com> Hello, all, I checked some data , which showed that Tween 20 could make bone sections flatten. But I am not sure of contrations(1%,2%,5%), time of incubation. If you have some experience in it , do you do me a favor? Thank you very much! Guofeng --------------------------------- Do You Yahoo!? ×¢²áÊÀ½çÒ»Á÷Æ·ÖʵÄÑÅ»¢Ãâ·ÑµçÓÊ From gcallis <@t> montana.edu Mon Apr 4 10:53:57 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Turtle embryo skulls sections slippage In-Reply-To: <803da5d8.e75c2a89.8237e00@mirapoint.jcu.edu> References: <803da5d8.e75c2a89.8237e00@mirapoint.jcu.edu> Message-ID: <6.0.0.22.1.20050404094412.01b8b480@gemini.msu.montana.edu> Frank, After picking up paraffin sections onto any kind of positive charged surface, you should let the slides drain vertically immediately after pickup . The water must drain away, from under the sections. Your sections should flatten on a waterbath adequately if you use a proper waterbath temperature for your given paraffin (this can vary a few degrees for any given paraffin). Don't even use a slide warmer, vertical slide holders are available - polyethylene boards with slanted slots, hold up to 70 or more slides. If you observe what happens, water tends to pool underneath a section. Good drainage will prevent this. After draining water away, go to a slide dryer. If the water still looks pooled at section edge (after draining for a time) you can release it with a sharp point or razor blade, wick it away. When using a warm waterbath, hopefully you never put additional adhesive in the waterbath. Adhesives in water coat the plus charge and negate its effects to make a section stick to the slide. Use pure distilled water, no adhesives. Good luck At 01:48 PM 4/3/2005, you wrote: >Hello, >I have been serially sectioning turtle embryo skulls embedded >in Paraplast. The ribbons are being mounted on >UltraStick/Ultrafrost Adhesion slides in a hot water-bath, >and the slides placed on a slide warmer. Most sections >remain intact in the position they were cut, but it seems >that random sections shift in position while sitting on the >slide warmer. I am not sure why this is occurring. Could it >be the result of water between the tissue and the slide? Any >advice on how to avoid this would be appreciated. >Thanks, >Frank > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From settembr <@t> umdnj.edu Mon Apr 4 10:48:24 2005 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] BAF47 Message-ID: Hello Megan, I purchased BAF47 through BD Transduction Laboratories, Cat.# 612110 or 612111 ( the difference is the amount) www.bdbiosciences.com Santa Cruz Biotechnnologies has it to. They are in Santa Cruz, California www.scbt.com Cat# sc-13055 Dana Settembre University Hospital - UMDNJ Newark, NJ USA >>> Megan Clarke 4/3/2005 10:54:35 PM >>> Hi Histonetters, Would like your help.We are trying to find an antibody BAF47/SNF5-anti-INI1 Can anyone please help us in the search for this. We would like it for formalin fixed parrafin embedded sections. Thank you for your help Megan Clarke Immunohistochemistry Lab HAPS,Newcastle AUSTRALIA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Apr 4 11:09:34 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] unsuccessful PNA staining In-Reply-To: <20050404094415.DC5A1CA099@ws7-4.us4.outblaze.com> References: <20050404094415.DC5A1CA099@ws7-4.us4.outblaze.com> Message-ID: <6.0.0.22.1.20050404095909.01b9eec0@gemini.msu.montana.edu> The method I sent is the one that comes from the Lectin Histochemistry (Brook, Leathem and Schumacher ISBN# 1 85996 100 2) book and also from a workshop by Barb Wright (a magician at immuno and lectin staining). Her method was taken from this book. What I sent to you are guidelines, and you may need to work with concentrations (lectin-HRP), incubations, etc to make it work. If you tell us exactly how you did the staining, then more suggestions can be made especially from others who do FFPE lectin work. My successful lectin staining has been done on frozen sections and NOT FFPE and I used Vectors lectin. The book, by the way, is inexpensive and wonderful source of information. At 03:44 AM 4/4/2005, you wrote: >Hi Gayle, > >I have been unsuccessful to stain germinal centres from bovine spleen and >lymphnodes (formalin fixed parafin embedded sections)with lectin >conjuagated to HRP as per the protocol suggetsed by you. Any suggestions, >please. >Thanks. >SV >-- Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Mon Apr 4 11:12:33 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: Frozen sections fixed or unfixed for IFA In-Reply-To: <42511198.29D397E2@uctgsh1.uct.ac.za> References: <42511198.29D397E2@uctgsh1.uct.ac.za> Message-ID: <6.0.0.22.1.20050404101010.01b978f0@gemini.msu.montana.edu> WE do it both ways with success but HOW you store and handle your sections is important. I think we get our best fluorescent staining on fresh FS air dried overnight, then fixed the next day followed by immediate IFA staining. The sections MUST be dry before fixation, no matter which way you handle them. At 04:06 AM 4/4/2005, you wrote: >Hi >Asking this question for a colleague who does the flourescent staining >on frozen sections.Does it make a difference to fluorescent staining if >one fixes cryosections in acetone before freezing the slides at -80?C? >Is it better to rather store the sections unfixed before freezing? Thank >you >Marilyn > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From shive003 <@t> umn.edu Mon Apr 4 11:18:18 2005 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] unsuccessful PNA staining References: <20050404094415.DC5A1CA099@ws7-4.us4.outblaze.com> <6.0.0.22.1.20050404095909.01b9eec0@gemini.msu.montana.edu> Message-ID: <010b01c53931$e9e7a3e0$41065486@auxs.umn.edu> There may also be a difference in the type and/or amounts of lectins expressed by species other than humans (I assume the book was about human lectin staining). I found this to be true when trying to stain non-primate mammalian endothelium with Ulex europaeus. The inquirer should first be sure that the glycoprotein he's trying to label on the bovine spleen/lymph node cells with PNA lectin is known to be present there. ----- Original Message ----- From: "Gayle Callis" To: "Subhash Verma" ; Sent: Monday, April 04, 2005 11:09 AM Subject: [Histonet] unsuccessful PNA staining > The method I sent is the one that comes from the Lectin Histochemistry > (Brook, Leathem and Schumacher ISBN# 1 85996 100 2) book and also from a > workshop by Barb Wright (a magician at immuno and lectin staining). Her > method was taken from this book. What I sent to you are guidelines, and > you may need to work with concentrations (lectin-HRP), incubations, etc to > make it work. > > If you tell us exactly how you did the staining, then more suggestions can > be made especially from others who do FFPE lectin work. My successful > lectin staining has been done on frozen sections and NOT FFPE and I used > Vectors lectin. > > The book, by the way, is inexpensive and wonderful source of information. > > > > At 03:44 AM 4/4/2005, you wrote: > >Hi Gayle, > > > >I have been unsuccessful to stain germinal centres from bovine spleen and > >lymphnodes (formalin fixed parafin embedded sections)with lectin > >conjuagated to HRP as per the protocol suggetsed by you. Any suggestions, > >please. > >Thanks. > >SV > >-- > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Sue.Kapoor <@t> uhsi.org Mon Apr 4 11:31:17 2005 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Vendors I need quotes Message-ID: <61E9F2400F53D5119CFC00508B44E33B02E9E0C0@khmcexch.uhsi.org> To all vendors, I am preparing my budget for next year and I'm looking for quotes for continuous feed slide stainers - new and used. Please email me at: sue.kapoor@uhsi.org Thank you, Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 Thank you! From krat18 <@t> aol.com Mon Apr 4 12:03:40 2005 From: krat18 <@t> aol.com (krat18@aol.com) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Good procedure for Cat Scratch Fever? In-Reply-To: <20050404145138.84370.qmail@web50308.mail.yahoo.com> References: <20050404145138.84370.qmail@web50308.mail.yahoo.com> Message-ID: <8C7074F6233F870-2B0-29333@mblk-r25.sysops.aol.com> We're looking for a good procedure to demonstrate Cat Scratch organisms. Could anyone out there please help us out? Are you using a modified Steiner stain for that? Please send along your procedure, if possible. Thanks. Karen krat18@aol.com From gcallis <@t> montana.edu Mon Apr 4 12:12:21 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Marking pens that WORK! Message-ID: <6.0.0.22.1.20050404110140.01b28e30@gemini.msu.montana.edu> Dear All, We just tried some new marking pens that actually stay on the plastic cassette during processing and superfrost slides during staining. The rep was kind enough to send sample, tested them on two different brand cassettes, and Erie Superfrost slides. They do NOT dry out after a few uses, and have a superb fine tip for marking. We did not try them under special staining conditions i.e i.e exposing slides to acids i.e. formic acid treatment of prion tissue sections before staining, or Bouins mordant for Massons trichrome. Something to keep in mind and test out. www.marketlabinc.com, QL0479 Moist Mark plus markers 10/pk for $36. Call them at 800 237-3604 and ask for a sample to try for your particular brand of cassette or slide, or special staining needs Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Charles.Embrey <@t> carle.com Mon Apr 4 12:56:18 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:54 2005 Subject: FW: Re: [Histonet] Re: ASCP - My 2 cents Message-ID: -----Original Message----- From: Charles.Embrey Sent: Monday, April 04, 2005 12:56 PM To: 'Chung, Luong' Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents What do you think a doctor's residency is? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Monday, April 04, 2005 10:21 AM To: Larry Woody; Histonet (E-mail) Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents You wouldn't go to see an OJT Doctors or nurses would you? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry Woody Sent: Monday, April 04, 2005 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents Bravo Ian: The problem here in the US is that there are thousands upon thousands of people working in histology labs that are not registered and don't have the theory behind histology and probably never will, and that is just the way some institutions and organizations want to keep it. That is not to say that some of these people are not dedicated to their jobs but if your in the field why not get the registry. Larry --------------------------------- Do you Yahoo!? Take Yahoo! Mail with you! Get it on your mobile phone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lchung <@t> ppmh.org Mon Apr 4 13:00:23 2005 From: lchung <@t> ppmh.org (Chung, Luong) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: He has an M.D. behind his name. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Monday, April 04, 2005 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: FW: Re: [Histonet] Re: ASCP - My 2 cents -----Original Message----- From: Charles.Embrey Sent: Monday, April 04, 2005 12:56 PM To: 'Chung, Luong' Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents What do you think a doctor's residency is? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Monday, April 04, 2005 10:21 AM To: Larry Woody; Histonet (E-mail) Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents You wouldn't go to see an OJT Doctors or nurses would you? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry Woody Sent: Monday, April 04, 2005 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents Bravo Ian: The problem here in the US is that there are thousands upon thousands of people working in histology labs that are not registered and don't have the theory behind histology and probably never will, and that is just the way some institutions and organizations want to keep it. That is not to say that some of these people are not dedicated to their jobs but if your in the field why not get the registry. Larry --------------------------------- Do you Yahoo!? Take Yahoo! Mail with you! Get it on your mobile phone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Mon Apr 4 13:07:18 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: Ok, someone right out of medical school has the M.D. after their name. Now would you let them remove your gallbladder without assistance before they do their (OJT)residency in surgery. Sure the degree is about the knowledge and theory but the OJT is about the practice. I for one think that a true professional has an ample helping of both. Chuck -----Original Message----- From: Chung, Luong [mailto:lchung@ppmh.org] Sent: Monday, April 04, 2005 1:00 PM To: Charles.Embrey; histonet@lists.utsouthwestern.edu Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents He has an M.D. behind his name. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Monday, April 04, 2005 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: FW: Re: [Histonet] Re: ASCP - My 2 cents -----Original Message----- From: Charles.Embrey Sent: Monday, April 04, 2005 12:56 PM To: 'Chung, Luong' Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents What do you think a doctor's residency is? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Monday, April 04, 2005 10:21 AM To: Larry Woody; Histonet (E-mail) Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents You wouldn't go to see an OJT Doctors or nurses would you? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry Woody Sent: Monday, April 04, 2005 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents Bravo Ian: The problem here in the US is that there are thousands upon thousands of people working in histology labs that are not registered and don't have the theory behind histology and probably never will, and that is just the way some institutions and organizations want to keep it. That is not to say that some of these people are not dedicated to their jobs but if your in the field why not get the registry. Larry --------------------------------- Do you Yahoo!? Take Yahoo! Mail with you! Get it on your mobile phone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Mon Apr 4 13:07:27 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: Or She. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Monday, April 04, 2005 2:00 PM To: Charles.Embrey; histonet@lists.utsouthwestern.edu Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents He has an M.D. behind his name. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Monday, April 04, 2005 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: FW: Re: [Histonet] Re: ASCP - My 2 cents -----Original Message----- From: Charles.Embrey Sent: Monday, April 04, 2005 12:56 PM To: 'Chung, Luong' Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents What do you think a doctor's residency is? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Monday, April 04, 2005 10:21 AM To: Larry Woody; Histonet (E-mail) Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents You wouldn't go to see an OJT Doctors or nurses would you? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry Woody Sent: Monday, April 04, 2005 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents Bravo Ian: The problem here in the US is that there are thousands upon thousands of people working in histology labs that are not registered and don't have the theory behind histology and probably never will, and that is just the way some institutions and organizations want to keep it. That is not to say that some of these people are not dedicated to their jobs but if your in the field why not get the registry. Larry --------------------------------- Do you Yahoo!? Take Yahoo! Mail with you! Get it on your mobile phone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbroomal <@t> NEMOURS.ORG Mon Apr 4 13:24:32 2005 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A798E@wlmmsx01.nemours.org> Kinda reminds me of the free labor, I mean clinical internship portion of my HT program. Kristen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Monday, April 04, 2005 2:07 PM To: Chung, Luong Cc: histonet@lists.utsouthwestern.edu Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents Ok, someone right out of medical school has the M.D. after their name. Now would you let them remove your gallbladder without assistance before they do their (OJT)residency in surgery. Sure the degree is about the knowledge and theory but the OJT is about the practice. I for one think that a true professional has an ample helping of both. Chuck From ohenry <@t> dfw.net Mon Apr 4 13:41:29 2005 From: ohenry <@t> dfw.net (Susan Owens) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Rodney Avila,HT(ASCP) Message-ID: <003301c53945$ebd727c0$b7dd3040@Nationwide.net> It is with great sadness that I tell you all of the 'passing' Saturday(after a brief illness) of our friend and colleague Rodney Avila. Rodney, a fine young man (age 36), is originally from San Antonio Texas and now will return there to be buried, in a military service, on Wednesday.(Plans not yet final.) Rodney has been working these past couple of years in Dallas Texas and in Arlington Texas. Anyone who wishes the funeral home address please email privately. Susan Owens-TX ohenry@dfw.net voice: 817-261-7938 From juan.gutierrez <@t> christushealth.org Mon Apr 4 14:04:22 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Good procedure for Cat Scratch Fever? Message-ID: We are using the Biocare immuno for it. Much cleaner and more specific. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of krat18@aol.com Sent: Monday, April 04, 2005 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good procedure for Cat Scratch Fever? We're looking for a good procedure to demonstrate Cat Scratch organisms. Could anyone out there please help us out? Are you using a modified Steiner stain for that? Please send along your procedure, if possible. Thanks. Karen krat18@aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Mon Apr 4 14:34:31 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4212@fh2k093.fhmis.net> Bravo, Jeanine!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: Chung, Luong; Charles.Embrey; histonet@lists.utsouthwestern.edu Sent: 4/4/2005 2:07 PM Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents Or She. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Monday, April 04, 2005 2:00 PM To: Charles.Embrey; histonet@lists.utsouthwestern.edu Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents He has an M.D. behind his name. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Monday, April 04, 2005 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: FW: Re: [Histonet] Re: ASCP - My 2 cents -----Original Message----- From: Charles.Embrey Sent: Monday, April 04, 2005 12:56 PM To: 'Chung, Luong' Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents What do you think a doctor's residency is? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Monday, April 04, 2005 10:21 AM To: Larry Woody; Histonet (E-mail) Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents You wouldn't go to see an OJT Doctors or nurses would you? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry Woody Sent: Monday, April 04, 2005 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents Bravo Ian: The problem here in the US is that there are thousands upon thousands of people working in histology labs that are not registered and don't have the theory behind histology and probably never will, and that is just the way some institutions and organizations want to keep it. That is not to say that some of these people are not dedicated to their jobs but if your in the field why not get the registry. Larry --------------------------------- Do you Yahoo!? Take Yahoo! Mail with you! Get it on your mobile phone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RCazares <@t> schosp.org Mon Apr 4 15:13:02 2005 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: My 2 cents Message-ID: <913FAC2B773C19488E26AE6572180FA501982551@exch01.schosp.org> Kelly, It is those people that are inquisitive, demanding and ambitious that will in fact go back to school seeking a degree. It is because they do wish to better themselves that they will find the time for even one class at a time, juggling jobs, family and other responsibilities. There are many of us out there, and yes, it may take us 10 years or more going part time, but we did it. And so can others. Ruth Cazares *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From kelly.mcqueeney <@t> bms.com Mon Apr 4 15:38:03 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: My 2 cents In-Reply-To: <913FAC2B773C19488E26AE6572180FA501982551@exch01.schosp.org> References: <913FAC2B773C19488E26AE6572180FA501982551@exch01.schosp.org> Message-ID: <4251A5AB.9060903@bms.com> Responding to the initial email... MY point was...2 degrees are not enough? Cazares, Ruth wrote: >Kelly, > > > >It is those people that are inquisitive, demanding and ambitious that >will in fact go back to school seeking a degree. It is because they do >wish to better themselves that they will find the time for even one >class at a time, juggling jobs, family and other responsibilities. >There are many of us out there, and yes, it may take us 10 years or more >going part time, but we did it. And so can others. > > > >Ruth Cazares > > > > > >*** Confidentiality Statement *** >This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. > > >Thank you for your cooperation. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From cfockler <@t> mail1.vcu.edu Mon Apr 4 18:15:20 2005 From: cfockler <@t> mail1.vcu.edu (cfockler@mail1.vcu.edu) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: My 2 cents In-Reply-To: <4251A5AB.9060903@bms.com> Message-ID: <200504042315.TAA01300@arrakis.vcu.edu> I said it once and I'll say it again, everyone keeps talking about "going back" and getting an education. . what about the ones coming in with a degree? Is my education not worth something from the beginning? Am I not as good a tech or employee if I haven't paid someone an arm and a leg to take a "test" to say I am proficient at my job? A lot of people are good at taking "tests" but are horrible at their job! And a lot of people are horrible test takers, but are some of the most valuable employees I have seen. Where's the line? Where's the consistency? Candyce ------------------- > Responding to the initial email... MY point was...2 degrees are not enough? > > Cazares, Ruth wrote: > > >Kelly, > > > > > > > >It is those people that are inquisitive, demanding and ambitious that > >will in fact go back to school seeking a degree. It is because they do > >wish to better themselves that they will find the time for even one > >class at a time, juggling jobs, family and other responsibilities. > >There are many of us out there, and yes, it may take us 10 years or more > >going part time, but we did it. And so can others. > > > > > > > >Ruth Cazares > > > > > > > > > > > >*** Confidentiality Statement *** > >This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. > > > > > >Thank you for your cooperation. > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Candyce Fockler Histotechnician Anatomic Pathology From jnocito <@t> satx.rr.com Mon Apr 4 20:31:41 2005 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents References: Message-ID: <00c101c5397f$3a68be60$7929f318@yourxhtr8hvc4p> Okay, this may be a little out range, but I'm trying to become a Pathologists' Assistant doing the OJT route. Does that make me any less professional that someone who completes an accredited program? Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Charles.Embrey" To: "Chung, Luong" Cc: Sent: Monday, April 04, 2005 1:07 PM Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents Ok, someone right out of medical school has the M.D. after their name. Now would you let them remove your gallbladder without assistance before they do their (OJT)residency in surgery. Sure the degree is about the knowledge and theory but the OJT is about the practice. I for one think that a true professional has an ample helping of both. Chuck -----Original Message----- From: Chung, Luong [mailto:lchung@ppmh.org] Sent: Monday, April 04, 2005 1:00 PM To: Charles.Embrey; histonet@lists.utsouthwestern.edu Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents He has an M.D. behind his name. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Monday, April 04, 2005 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: FW: Re: [Histonet] Re: ASCP - My 2 cents -----Original Message----- From: Charles.Embrey Sent: Monday, April 04, 2005 12:56 PM To: 'Chung, Luong' Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents What do you think a doctor's residency is? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Monday, April 04, 2005 10:21 AM To: Larry Woody; Histonet (E-mail) Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents You wouldn't go to see an OJT Doctors or nurses would you? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry Woody Sent: Monday, April 04, 2005 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents Bravo Ian: The problem here in the US is that there are thousands upon thousands of people working in histology labs that are not registered and don't have the theory behind histology and probably never will, and that is just the way some institutions and organizations want to keep it. That is not to say that some of these people are not dedicated to their jobs but if your in the field why not get the registry. Larry --------------------------------- Do you Yahoo!? Take Yahoo! Mail with you! Get it on your mobile phone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Anti-Virus. Version: 7.0.308 / Virus Database: 266.9.1 - Release Date: 4/1/2005 From bruno.lectard <@t> cea.fr Tue Apr 5 08:56:50 2005 From: bruno.lectard <@t> cea.fr (LECTARD Bruno 172070) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Frozen section Message-ID: <014DAB1C8A8DD611B60F0008C75DB91C0522D351@oldtrafford.far.cea.fr> Hi all, I looking for a protocol for frozen rat tissus. Can you help me Bruno From Charles.Embrey <@t> carle.com Tue Apr 5 09:23:51 2005 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: Joe, Once you finish your OJT requirements, take the certification exam and become a PA you will see that the OJT vs Program trained debate is alive and well within the PA ranks. I think many people within the AAPA and also on histonet fail to realize that OJT stands for On The Job TRAINING. It is simply another tool to teach a profession. The problem is that where NAACLS approved programs are all very similar in the content and level of instruction, OJT programs are not regulated and can differ greatly. This is where certification helps to level the playing field. Everyone, program trained or OJT must be able to prove a certain knowledge base. Even in a very hands on profession as Histology the required knowledge base of twenty years ago is hardly adequate today. For the PA exam- Study, study and re-study your Robbins. The knowledge base is vast and all areas are covered in the exam. I have heard people complain that they are not good test takers. Well, in my experience, most people that fail tests just aren't prepared. Degrees are great and proper OJT is invaluable but even pathologists have to follow their med school and residency with a very tough board exam. I don't think I want a pathologist, that failed his boards, reading my slide with the excuse: "I'm a good pathologist but I failed my boards because I'm not a good test taker." I know you and your experience level Joe. Trust me; you will make a fine and professional (even if a bit vocal) PA. Just remember, ROBBINS-ROBBINS-ROBBINS. Chuck -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Monday, April 04, 2005 8:32 PM To: Charles.Embrey; Chung, Luong Cc: histonet@lists.utsouthwestern.edu Subject: Re: Re: [Histonet] Re: ASCP - My 2 cents Okay, this may be a little out range, but I'm trying to become a Pathologists' Assistant doing the OJT route. Does that make me any less professional that someone who completes an accredited program? Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Charles.Embrey" To: "Chung, Luong" Cc: Sent: Monday, April 04, 2005 1:07 PM Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents Ok, someone right out of medical school has the M.D. after their name. Now would you let them remove your gallbladder without assistance before they do their (OJT)residency in surgery. Sure the degree is about the knowledge and theory but the OJT is about the practice. I for one think that a true professional has an ample helping of both. Chuck -----Original Message----- From: Chung, Luong [mailto:lchung@ppmh.org] Sent: Monday, April 04, 2005 1:00 PM To: Charles.Embrey; histonet@lists.utsouthwestern.edu Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents He has an M.D. behind his name. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Monday, April 04, 2005 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: FW: Re: [Histonet] Re: ASCP - My 2 cents -----Original Message----- From: Charles.Embrey Sent: Monday, April 04, 2005 12:56 PM To: 'Chung, Luong' Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents What do you think a doctor's residency is? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Monday, April 04, 2005 10:21 AM To: Larry Woody; Histonet (E-mail) Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents You wouldn't go to see an OJT Doctors or nurses would you? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry Woody Sent: Monday, April 04, 2005 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents Bravo Ian: The problem here in the US is that there are thousands upon thousands of people working in histology labs that are not registered and don't have the theory behind histology and probably never will, and that is just the way some institutions and organizations want to keep it. That is not to say that some of these people are not dedicated to their jobs but if your in the field why not get the registry. Larry --------------------------------- Do you Yahoo!? Take Yahoo! Mail with you! Get it on your mobile phone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Anti-Virus. Version: 7.0.308 / Virus Database: 266.9.1 - Release Date: 4/1/2005 From Dorothy.L.Webb <@t> HealthPartners.Com Tue Apr 5 09:40:11 2005 From: Dorothy.L.Webb <@t> HealthPartners.Com (Dorothy.L.Webb@HealthPartners.Com) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] nails Message-ID: There is a product from Sigma called "Tween" that works great at both softening and fixation. It is a 5% solution in 10% formalin and stays in there for 24 hours and then processed routinely, cut and placed on a charged slide and we only stain for PAS unless it has the nailbed with it. The order number is P4634. Good luck! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From mauger <@t> email.chop.edu Tue Apr 5 09:43:09 2005 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] BAF47 Message-ID: Megan, You can get it from BD Transduction Labs #612111. Jo Mauger >>> "Megan Clarke" 04/03/05 10:54 PM >>> Hi Histonetters, Would like your help.We are trying to find an antibody BAF47/SNF5-anti-INI1 Can anyone please help us in the search for this. We would like it for formalin fixed parrafin embedded sections. Thank you for your help Megan Clarke Immunohistochemistry Lab HAPS,Newcastle AUSTRALIA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> elht.nhs.uk Tue Apr 5 09:48:44 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] nails[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F143@bhrv-nt-11.bhrv.nwest.nhs.uk> Fabric softener apparently does it to. Dunno which one, but it apparently works; wonders if its got Tween in it too? -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: 05 April 2005 15:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nails[Scanned] There is a product from Sigma called "Tween" that works great at both softening and fixation. It is a 5% solution in 10% formalin and stays in there for 24 hours and then processed routinely, cut and placed on a charged slide and we only stain for PAS unless it has the nailbed with it. The order number is P4634. Good luck! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Tue Apr 5 09:59:37 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] nails[Scanned] Message-ID: This business of softening nails continues to intrigue me. It seems that the only common factor (in all the various protocols) is water, and that what you are doing is hydrating the nail. Of course, you then process and dehydrate it. It remains a mystery. I remain unconvinced at the utility of any method. I our hands, nails stay as hard as - well - nails I guess. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 05 April 2005 15:49 To: 'Dorothy.L.Webb@HealthPartners.Com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] Fabric softener apparently does it to. Dunno which one, but it apparently works; wonders if its got Tween in it too? -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: 05 April 2005 15:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nails[Scanned] There is a product from Sigma called "Tween" that works great at both softening and fixation. It is a 5% solution in 10% formalin and stays in there for 24 hours and then processed routinely, cut and placed on a charged slide and we only stain for PAS unless it has the nailbed with it. The order number is P4634. Good luck! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathrm35 <@t> adelphia.net Tue Apr 5 10:03:01 2005 From: pathrm35 <@t> adelphia.net (pathrm35@adelphia.net) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Physician office/lab computer systems Message-ID: <24574076.1112713381703.JavaMail.root@web7.mail.adelphia.net> Does anyone know of a good computer software system designed specifically for a dermatology practice (several offices w/ a small lab) ? I am not looking for a hospital based LIS as we are too small for that. Please feel free to contact me as I do have some questions. Any company reps please feel free as well. Thanks, Ron Martin 561-721-2400 From Kemlo.Rogerson <@t> elht.nhs.uk Tue Apr 5 10:06:09 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] nails[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F147@bhrv-nt-11.bhrv.nwest.nhs.uk> Don't think it's just water, I assuming it's something dead scientific that alters the chemical bonding between proteins; like hydrolysis or something. I mean what does Fabric conditioner have in it that makes cloths soft? No I think it's something dead scientific, but I'll be beggared if I know. -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 05 April 2005 16:00 To: Kemlo Rogerson; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] This business of softening nails continues to intrigue me. It seems that the only common factor (in all the various protocols) is water, and that what you are doing is hydrating the nail. Of course, you then process and dehydrate it. It remains a mystery. I remain unconvinced at the utility of any method. I our hands, nails stay as hard as - well - nails I guess. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 05 April 2005 15:49 To: 'Dorothy.L.Webb@HealthPartners.Com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] Fabric softener apparently does it to. Dunno which one, but it apparently works; wonders if its got Tween in it too? -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: 05 April 2005 15:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nails[Scanned] There is a product from Sigma called "Tween" that works great at both softening and fixation. It is a 5% solution in 10% formalin and stays in there for 24 hours and then processed routinely, cut and placed on a charged slide and we only stain for PAS unless it has the nailbed with it. The order number is P4634. Good luck! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Tue Apr 5 10:07:23 2005 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents In-Reply-To: References: Message-ID: <1112713643.4252a9ab2fbc3@imp.vet.upenn.edu> I have been watching this debate for over a week now and feel it has added to a good debate and insulted some very good histologist registered, un-registered, with or without degrees and OJT. I am a registered histologist who was trained on the job while in college many years ago. My training was excellent and more research in the beginning than clinical (I have done both). I also spent a number of years in industry and can say I have friends who fall into all categories listed. I know some histologist who were OJT and are better and more knowledgeable than some with degrees and vice versa. The issue we are overlooking is the fact that we have all of these people in Histology and Pathology now and not all have the same opportunities of being in cities or near places with programs. Many hospitals will not pay to send a histologist to a local meeting in town much less state, region or national for more training and to get CEUs. While I know this will fall on some deaf ears not everyone can afford to go and pay out of pocket or for a teleconference when the boss or hospital says NO. This is the real issue and the problem we need to face. The ASCP is our licensing body and yet they have had little interest in where we train or how. It has been to the pathologist advantage to OJT as the salaries could be kept lower and we were not required to have degrees or in some cases register. We do need more education and understanding now as the field has progressed past just special stains and H&Es to immunos. Many who are being trained OJT are not learning basic chemistry of stains but how to order them and who to call in technical service when they fail instead of how to make them. Real training in chemistry and biology in general is needed and we should see what NSH and the ASCP can do to help. If we are not being heard then we need to bring it in louder that is how blood drawing became a field instead of a call for anyone with the touch in the clinical laboratory. Our field is much more complex!! These are critical times and I for one do not want to go back to the old days when IHC first came in and the pathologists wanted to transfer everything to hematology as they were degreed and knfew more. They could not ix tissue and did not understand anything about tissue and most histology laboratories got IHC back within a ew years. Now we could be looking at some of these same ideas again. Don't just fight education help figure out how to make it available to anyone who wants to learn our field and go and recruit at the high schools or community colleges where programs are becoming available. We don't do enough for ourselves to advertise who we are. Off the Soap Box and joining Joe in trouble. Pam Marcum Quoting "Charles.Embrey" : > Joe, Once you finish your OJT requirements, take the certification exam > and become a PA you will see that the OJT vs Program trained debate is > alive and well within the PA ranks. I think many people within the AAPA > and also on histonet fail to realize that OJT stands for On The Job > TRAINING. It is simply another tool to teach a profession. The problem > is that where NAACLS approved programs are all very similar in the > content and level of instruction, OJT programs are not regulated and can > differ greatly. This is where certification helps to level the playing > field. Everyone, program trained or OJT must be able to prove a certain > knowledge base. Even in a very hands on profession as Histology the > required knowledge base of twenty years ago is hardly adequate today. > For the PA exam- Study, study and re-study your Robbins. The knowledge > base is vast and all areas are covered in the exam. I have heard people > complain that they are not good test takers. Well, in my experience, > most people that fail tests just aren't prepared. Degrees are great and > proper OJT is invaluable but even pathologists have to follow their med > school and residency with a very tough board exam. I don't think I want > a pathologist, that failed his boards, reading my slide with the excuse: > "I'm a good pathologist but I failed my boards because I'm not a good > test taker." > I know you and your experience level Joe. Trust me; you will make a > fine and professional (even if a bit vocal) PA. Just remember, > ROBBINS-ROBBINS-ROBBINS. > > Chuck > > > -----Original Message----- > From: Joe Nocito [mailto:jnocito@satx.rr.com] > Sent: Monday, April 04, 2005 8:32 PM > To: Charles.Embrey; Chung, Luong > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: Re: [Histonet] Re: ASCP - My 2 cents > > Okay, > this may be a little out range, but I'm trying to become a > Pathologists' > Assistant doing the OJT route. Does that make me any less professional > that > someone who completes an accredited program? > > Joe Nocito BS, HT(ASCP)QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > ----- Original Message ----- > From: "Charles.Embrey" > To: "Chung, Luong" > Cc: > Sent: Monday, April 04, 2005 1:07 PM > Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents > > > Ok, someone right out of medical school has the M.D. after their name. > Now would you let them remove your gallbladder without assistance before > they do their (OJT)residency in surgery. Sure the degree is about the > knowledge and theory but the OJT is about the practice. I for one think > that a true professional has an ample helping of both. > Chuck > > -----Original Message----- > From: Chung, Luong [mailto:lchung@ppmh.org] > Sent: Monday, April 04, 2005 1:00 PM > To: Charles.Embrey; histonet@lists.utsouthwestern.edu > Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents > > He has an M.D. behind his name. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of > Charles.Embrey > Sent: Monday, April 04, 2005 1:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: FW: Re: [Histonet] Re: ASCP - My 2 cents > > > > > -----Original Message----- > From: Charles.Embrey > Sent: Monday, April 04, 2005 12:56 PM > To: 'Chung, Luong' > Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents > > What do you think a doctor's residency is? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, > Luong > Sent: Monday, April 04, 2005 10:21 AM > To: Larry Woody; Histonet (E-mail) > Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents > > You wouldn't go to see an OJT Doctors or nurses would you? > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry > Woody > Sent: Monday, April 04, 2005 11:03 AM > To: histonet@lists.utsouthwestern.edu > Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents > > > Bravo Ian: The problem here in the US is that there are thousands upon > thousands of people working in histology labs that are not registered > and don't have the theory behind histology and probably never will, and > that is just the way some institutions and organizations want to keep > it. That is not to say that some of these people are not dedicated to > their jobs but if your in the field why not get the registry. Larry > > > --------------------------------- > Do you Yahoo!? > Take Yahoo! Mail with you! Get it on your mobile phone. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Anti-Virus. > Version: 7.0.308 / Virus Database: 266.9.1 - Release Date: 4/1/2005 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Terry.Marshall <@t> rothgen.nhs.uk Tue Apr 5 10:22:46 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] nails[Scanned] Message-ID: What makes clothes soft doesn't necessarily make nails soft. Fabric conditioners work by fluffing out fibres, or at least, stopping them matting. There be no fibres in toe nails (unless you have a fungal infection). Some swear by decalcifying fluid, though there be no calcium in nails either. But, there be water in decalcifying fluid. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 05 April 2005 16:06 To: Marshall Terry Dr, Consultant Histopathologist; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] Don't think it's just water, I assuming it's something dead scientific that alters the chemical bonding between proteins; like hydrolysis or something. I mean what does Fabric conditioner have in it that makes cloths soft? No I think it's something dead scientific, but I'll be beggared if I know. -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 05 April 2005 16:00 To: Kemlo Rogerson; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] This business of softening nails continues to intrigue me. It seems that the only common factor (in all the various protocols) is water, and that what you are doing is hydrating the nail. Of course, you then process and dehydrate it. It remains a mystery. I remain unconvinced at the utility of any method. I our hands, nails stay as hard as - well - nails I guess. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 05 April 2005 15:49 To: 'Dorothy.L.Webb@HealthPartners.Com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] Fabric softener apparently does it to. Dunno which one, but it apparently works; wonders if its got Tween in it too? -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: 05 April 2005 15:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nails[Scanned] There is a product from Sigma called "Tween" that works great at both softening and fixation. It is a 5% solution in 10% formalin and stays in there for 24 hours and then processed routinely, cut and placed on a charged slide and we only stain for PAS unless it has the nailbed with it. The order number is P4634. Good luck! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Tue Apr 5 10:30:43 2005 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and they insist that we need to use Superfrost Plus s Message-ID: We're talking of buying a Ventana Benchmark XT and they insist that we need to use Superfrost Plus slides. They are going to cost much more than what we're using now. Does anyone have the inside track on the least expensive place to buy them from? You folks are always "The" source for All Histology Wisdom! Thanks! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Dorothy.L.Webb <@t> HealthPartners.Com Tue Apr 5 10:31:41 2005 From: Dorothy.L.Webb <@t> HealthPartners.Com (Dorothy.L.Webb@HealthPartners.Com) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] antibody Message-ID: Does anyone know who performs IHC testing for rickettsieae..we checked with Mayo and Propath..any ideas and thanks!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Leslie.Chaussey <@t> uchospitals.edu Tue Apr 5 10:34:27 2005 From: Leslie.Chaussey <@t> uchospitals.edu (Leslie.Chaussey@uchospitals.edu) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] nails[Scanned] Message-ID: <4CB46920276D5047BEBDA04E67BBF59F024C8911@uchadmb03.uchad.uchospitals.edu> We use Nair to soften nails... I was told that it's a dekeratinizing agent. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Tuesday, April 05, 2005 10:23 AM To: Kemlo Rogerson; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] What makes clothes soft doesn't necessarily make nails soft. Fabric conditioners work by fluffing out fibres, or at least, stopping them matting. There be no fibres in toe nails (unless you have a fungal infection). Some swear by decalcifying fluid, though there be no calcium in nails either. But, there be water in decalcifying fluid. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 05 April 2005 16:06 To: Marshall Terry Dr, Consultant Histopathologist; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] Don't think it's just water, I assuming it's something dead scientific that alters the chemical bonding between proteins; like hydrolysis or something. I mean what does Fabric conditioner have in it that makes cloths soft? No I think it's something dead scientific, but I'll be beggared if I know. -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 05 April 2005 16:00 To: Kemlo Rogerson; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] This business of softening nails continues to intrigue me. It seems that the only common factor (in all the various protocols) is water, and that what you are doing is hydrating the nail. Of course, you then process and dehydrate it. It remains a mystery. I remain unconvinced at the utility of any method. I our hands, nails stay as hard as - well - nails I guess. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 05 April 2005 15:49 To: 'Dorothy.L.Webb@HealthPartners.Com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] Fabric softener apparently does it to. Dunno which one, but it apparently works; wonders if its got Tween in it too? -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: 05 April 2005 15:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nails[Scanned] There is a product from Sigma called "Tween" that works great at both softening and fixation. It is a 5% solution in 10% formalin and stays in there for 24 hours and then processed routinely, cut and placed on a charged slide and we only stain for PAS unless it has the nailbed with it. The order number is P4634. Good luck! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Hospitals ******************************************************************************** From STEGTM <@t> samcstl.org Tue Apr 5 10:48:49 2005 From: STEGTM <@t> samcstl.org (Therersa Stegall) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] ASCP - my 2 cents Message-ID: I concur. If you care about your work, continuing education, both in school and on the job (this "net" helps) is the way to stay current. From dholmes <@t> anatomy.umsmed.edu Tue Apr 5 10:49:29 2005 From: dholmes <@t> anatomy.umsmed.edu (Dianne Holmes) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] HELP! "alien" bubbles ? Message-ID: I have just completed an immuno rxn on rabbit brain and have come up with micro-bubbles that cant be de-hydrated? I mounted and stained one set of tissue with cresyl violet. No rxn was done on this set and they are perfect. Next day, I ran 2 sets through immunos. One with BDA and the other a BDA/PHAL . Dehydrated as usual , mounted with Cytoseal 60 and checking it under the scope (because these bubbles can not be seen while coverslipping) the sections are "surrounded" by bubbles and on the perpherals of the tissue but none on the inner cells? Repeated a couple of slides with a longer dehydration step and it made NO difference. I would expect to see 'less' of the bubbles but it was unchanged? Anyone have any idea what is going on?? From Terry.Marshall <@t> rothgen.nhs.uk Tue Apr 5 10:45:17 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] nails[Scanned] Message-ID: Been there done that too. Useless. Moreover it's illogical. Since nail is 100% keratin, if it worked, there would be no nail left:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Leslie.Chaussey@uchospitals.edu [mailto:Leslie.Chaussey@uchospitals.edu] Sent: 05 April 2005 16:34 To: Marshall Terry Dr, Consultant Histopathologist; Kemlo.Rogerson@elht.nhs.uk; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] We use Nair to soften nails... I was told that it's a dekeratinizing agent. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Tuesday, April 05, 2005 10:23 AM To: Kemlo Rogerson; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] What makes clothes soft doesn't necessarily make nails soft. Fabric conditioners work by fluffing out fibres, or at least, stopping them matting. There be no fibres in toe nails (unless you have a fungal infection). Some swear by decalcifying fluid, though there be no calcium in nails either. But, there be water in decalcifying fluid. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 05 April 2005 16:06 To: Marshall Terry Dr, Consultant Histopathologist; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] Don't think it's just water, I assuming it's something dead scientific that alters the chemical bonding between proteins; like hydrolysis or something. I mean what does Fabric conditioner have in it that makes cloths soft? No I think it's something dead scientific, but I'll be beggared if I know. -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 05 April 2005 16:00 To: Kemlo Rogerson; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] This business of softening nails continues to intrigue me. It seems that the only common factor (in all the various protocols) is water, and that what you are doing is hydrating the nail. Of course, you then process and dehydrate it. It remains a mystery. I remain unconvinced at the utility of any method. I our hands, nails stay as hard as - well - nails I guess. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 05 April 2005 15:49 To: 'Dorothy.L.Webb@HealthPartners.Com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] Fabric softener apparently does it to. Dunno which one, but it apparently works; wonders if its got Tween in it too? -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: 05 April 2005 15:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nails[Scanned] There is a product from Sigma called "Tween" that works great at both softening and fixation. It is a 5% solution in 10% formalin and stays in there for 24 hours and then processed routinely, cut and placed on a charged slide and we only stain for PAS unless it has the nailbed with it. The order number is P4634. Good luck! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Hospitals ******************************************************************************** From Kemlo.Rogerson <@t> elht.nhs.uk Tue Apr 5 10:54:07 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] nails[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F148@bhrv-nt-11.bhrv.nwest.nhs.uk> Hot off the press; Immac hair removal cream softens cos it breaks up keratin. That's scientific and you rub it on a leave it for a day or two. So how exactly does a fabric conditioner fluff up fibres then? Is it enzymic? -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 05 April 2005 16:23 To: Kemlo Rogerson; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] What makes clothes soft doesn't necessarily make nails soft. Fabric conditioners work by fluffing out fibres, or at least, stopping them matting. There be no fibres in toe nails (unless you have a fungal infection). Some swear by decalcifying fluid, though there be no calcium in nails either. But, there be water in decalcifying fluid. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 05 April 2005 16:06 To: Marshall Terry Dr, Consultant Histopathologist; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] Don't think it's just water, I assuming it's something dead scientific that alters the chemical bonding between proteins; like hydrolysis or something. I mean what does Fabric conditioner have in it that makes cloths soft? No I think it's something dead scientific, but I'll be beggared if I know. -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 05 April 2005 16:00 To: Kemlo Rogerson; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] This business of softening nails continues to intrigue me. It seems that the only common factor (in all the various protocols) is water, and that what you are doing is hydrating the nail. Of course, you then process and dehydrate it. It remains a mystery. I remain unconvinced at the utility of any method. I our hands, nails stay as hard as - well - nails I guess. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 05 April 2005 15:49 To: 'Dorothy.L.Webb@HealthPartners.Com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] Fabric softener apparently does it to. Dunno which one, but it apparently works; wonders if its got Tween in it too? -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: 05 April 2005 15:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nails[Scanned] There is a product from Sigma called "Tween" that works great at both softening and fixation. It is a 5% solution in 10% formalin and stays in there for 24 hours and then processed routinely, cut and placed on a charged slide and we only stain for PAS unless it has the nailbed with it. The order number is P4634. Good luck! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Tue Apr 5 11:05:27 2005 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] 4 cents worth Message-ID: <566FB0B522443D43AF02D2ADBE35A6F00163F8A7@UTHEVS3.mail.uthouston.edu> I must in general agree with Pam and others who have stressed the need for formal recognition of skills such as with a recognized program. I do not think that anyone has been insulted on Histonet with regard to their on the job training. I also had a lot of on the job training. What those of y'all who may be indignant when the quality of OJT is questioned have to realize is that it is not possible to equate OTJ in different workplaces. You may have had superb training or may have been in a job where the training was poor or mediocre at best. You may have been working your buts off or had time to improve your skills and experiment with new procedures. It is difficult to know what your specific situation is unless you have a piece of paper where you may be compared to other individuals. Having passed the ASCP exam does not necessarily mean that you are better trained than somewhat who has worked in a great lab and only received OJT. It does mean that you can be compared to others who have passed the ASCP exam, it is a basic level. Having a degree especially, outside the field may indicate that you have reached a certain level of academia, but also does not guarantee that you have "street smarts" or will be able to pick up techniques and concepts better than an individual who has not received such training. You only have to ask someone who has been placed in charge of helping graduate students with their histology projects to know how true this is. The ASCP exam, while it is not perfect is a first step in ensuring that individuals have the basics. If we are going to work on anything lets work on improving the ASCP examination. I personally have a lot of problems with the practical portion where automated machines are allowed to be used to prepare slides. Barry From la.sebree <@t> hosp.wisc.edu Tue Apr 5 11:09:54 2005 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and theyinsist that we need to use Superfrost Plus s Message-ID: Angela, You will definitely need "Plus" slides no matter whose instrument you use. Find out which laboratory supply company your institution has a contract with. They may be your least expensive choice. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Tuesday, April 05, 2005 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and theyinsist that we need to use Superfrost Plus s We're talking of buying a Ventana Benchmark XT and they insist that we need to use Superfrost Plus slides. They are going to cost much more than what we're using now. Does anyone have the inside track on the least expensive place to buy them from? You folks are always "The" source for All Histology Wisdom! Thanks! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Tue Apr 5 11:18:54 2005 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] 4 cents worth In-Reply-To: <566FB0B522443D43AF02D2ADBE35A6F00163F8A7@UTHEVS3.mail.uthouston.edu> Message-ID: <000001c539fb$29d051e0$3601a8c0@brownpathology.net> I agree, Barry!!! I don't think that I would want to submitt an automated slide. How can you take pride in that? Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, April 05, 2005 11:05 AM To: histonet Subject: [Histonet] 4 cents worth I must in general agree with Pam and others who have stressed the need for formal recognition of skills such as with a recognized program. I do not think that anyone has been insulted on Histonet with regard to their on the job training. I also had a lot of on the job training. What those of y'all who may be indignant when the quality of OJT is questioned have to realize is that it is not possible to equate OTJ in different workplaces. You may have had superb training or may have been in a job where the training was poor or mediocre at best. You may have been working your buts off or had time to improve your skills and experiment with new procedures. It is difficult to know what your specific situation is unless you have a piece of paper where you may be compared to other individuals. Having passed the ASCP exam does not necessarily mean that you are better trained than somewhat who has worked in a great lab and only received OJT. It does mean that you can be compared to others who have passed the ASCP exam, it is a basic level. Having a degree especially, outside the field may indicate that you have reached a certain level of academia, but also does not guarantee that you have "street smarts" or will be able to pick up techniques and concepts better than an individual who has not received such training. You only have to ask someone who has been placed in charge of helping graduate students with their histology projects to know how true this is. The ASCP exam, while it is not perfect is a first step in ensuring that individuals have the basics. If we are going to work on anything lets work on improving the ASCP examination. I personally have a lot of problems with the practical portion where automated machines are allowed to be used to prepare slides. Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Tue Apr 5 11:23:58 2005 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Sep 16 15:24:54 2005 Subject: FW: [Histonet] We're talking of buying a Ventana Benchmark XT andtheyinsist that we need to use Superfrost Plus s Message-ID: <000201c539fb$deaf92b0$3601a8c0@brownpathology.net> I think the issue is that Ventana is insisting on those specific slides.... Not just any old "Plus Slides". At least that is the issue I face when sending reference work out to a particular laboratory. I have been told that I have to cut them on only Fisher or VWR Superfrost Plus slides because of a problem that exists when using other slides on their particular instrumentation. I HOPE that this issue is being looked at closely!! (Ethel, can you comment here?) Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Tuesday, April 05, 2005 11:10 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XT andtheyinsist that we need to use Superfrost Plus s Angela, You will definitely need "Plus" slides no matter whose instrument you use. Find out which laboratory supply company your institution has a contract with. They may be your least expensive choice. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Tuesday, April 05, 2005 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and theyinsist that we need to use Superfrost Plus s We're talking of buying a Ventana Benchmark XT and they insist that we need to use Superfrost Plus slides. They are going to cost much more than what we're using now. Does anyone have the inside track on the least expensive place to buy them from? You folks are always "The" source for All Histology Wisdom! Thanks! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From John.Sheppard <@t> Health-Partners.org Tue Apr 5 11:22:22 2005 From: John.Sheppard <@t> Health-Partners.org (John.Sheppard@Health-Partners.org) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: Women have been using midwives in childbirth for eons, most had little or no formal training. OJT was the way for me! -Big Shep- HT(ASCP) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From gcallis <@t> montana.edu Tue Apr 5 11:35:21 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: HELP! "alien" bubbles ? In-Reply-To: References: Message-ID: <6.0.0.22.1.20050405100708.01b1da08@gemini.msu.montana.edu> Dianne, Two possibilities here: 1) Is the Cytoseal 60 in a plastic bottle that has the tiny lift up tab at top so you can dispense? If so, anytime time I have used these bottles for coverslipping, I get bubbles. When this bottle is tipped back and forth, you not only agitate the media, but the top allows air to get sucked into the media i.e. regurgitation? You can tip the bottle on its side to ensure the tip is always filled with media and no air gets back in, although this leads to leakage of media from tip - too messy! OR Use the old fashioned method (our favorite - a drop falling off of either a narrow glass rod, or applicator stick onto the section.). If you use applicator stick, it can be tossed when done. Just pour media into a bottle with tight lid. OR use the classic balsam bottle with glass rod - you can still get these from Fisher. We keep these around for huge staining projects. The other possibility is incompatibility of solvents although this may be an old wives tale - was told milleniums ago that using toluene based mounting media with sections coming out of xylene will cause bubbles - although we do not have many problems doing just this in our lab. If coverslipping out of a xylene substitute, i.e. Clearite 3, we make sure we have a mounting media compatible with that solvent. Check with manufacturer. Most mounting medias seem to be toluene based. If we need to thin a thickened media, we use the solvent it is dissolved in. ie. toluene, NOT xylene. Our Richard Allan toluene based mounting media is compatible with xylene and Clearite 3. Propar xylene substitutes, but we do use "drop off a stick" method to avoid bubbles. Instead of adding more dehydrants, add extra clearing station and be sure to coverslip out of one that is not close to dehydrant. We use 2 X clearing, and coverslip out of the 3rd clearant. If the sections are thicker, slightly longer times in dehyrants and clearing agents may be needed. My overall personal opinion is that goofy dispenser bottle is the culprit, and they are NOT used here anymore because we dislike them intensely. This is also a problem with aqueous mounting media dispenser bottles - we lay these on their side to keep tip filled and not with air. At 09:49 AM 4/5/2005, you wrote: >I have just completed an immuno rxn on rabbit brain and have come up >with micro-bubbles that cant be de-hydrated? I mounted and stained one >set of tissue with cresyl violet. No rxn was done on this set and they >are perfect. Next day, I ran 2 sets through immunos. One with BDA and >the other a BDA/PHAL . Dehydrated as usual , mounted with Cytoseal 60 >and checking it under the scope (because these bubbles can not be seen >while coverslipping) the sections are "surrounded" by bubbles and on >the perpherals of the tissue but none on the inner cells? Repeated a >couple of slides with a longer dehydration step and it made NO >difference. I would expect to see 'less' of the bubbles but it was >unchanged? Anyone have any idea what is going on?? > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Tue Apr 5 11:40:39 2005 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] 4 cents worth Message-ID: I disagree Bonnie. My co-worker and I have designed every protocol our automated immunostainers run and I do take pride in that. Although way back when (1976) when I did my practical, all my steps were manual. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie Whitaker Sent: Tuesday, April 05, 2005 11:19 AM To: 'histonet' Subject: RE: [Histonet] 4 cents worth I agree, Barry!!! I don't think that I would want to submitt an automated slide. How can you take pride in that? Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, April 05, 2005 11:05 AM To: histonet Subject: [Histonet] 4 cents worth I must in general agree with Pam and others who have stressed the need for formal recognition of skills such as with a recognized program. I do not think that anyone has been insulted on Histonet with regard to their on the job training. I also had a lot of on the job training. What those of y'all who may be indignant when the quality of OJT is questioned have to realize is that it is not possible to equate OTJ in different workplaces. You may have had superb training or may have been in a job where the training was poor or mediocre at best. You may have been working your buts off or had time to improve your skills and experiment with new procedures. It is difficult to know what your specific situation is unless you have a piece of paper where you may be compared to other individuals. Having passed the ASCP exam does not necessarily mean that you are better trained than somewhat who has worked in a great lab and only received OJT. It does mean that you can be compared to others who have passed the ASCP exam, it is a basic level. Having a degree especially, outside the field may indicate that you have reached a certain level of academia, but also does not guarantee that you have "street smarts" or will be able to pick up techniques and concepts better than an individual who has not received such training. You only have to ask someone who has been placed in charge of helping graduate students with their histology projects to know how true this is. The ASCP exam, while it is not perfect is a first step in ensuring that individuals have the basics. If we are going to work on anything lets work on improving the ASCP examination. I personally have a lot of problems with the practical portion where automated machines are allowed to be used to prepare slides. Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JNocito <@t> Pathreflab.com Tue Apr 5 11:40:49 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents In-Reply-To: <1112713643.4252a9ab2fbc3@imp.vet.upenn.edu> Message-ID: thanks to Pam and Chuck. Believe me, if I could go to an accredited program, I would. My pathologists are not willing to let me leave here for 2 years. They say I am a vital part of this company. My nights are filled with reading, reading and re-reading. I know what is at stake. My mentor pathologist is great at showing the basics of grossing large specimens and I am grateful. When I told him that now I know what it must have felt like as a first year pathology resident, he told me no I don't because I don't have 4 years of medical school behind me. He meant that since I'm doing this practically by myself without the benefit of medical school, he holds me in high regards just for attempting the exam. Whatever people think of me really doesn't matter when I have a boss that stands behind my decisions to improve myself. OJT or not, in his eyes, I am more professional than some of his peers. I can deal with that. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of pmarcum@vet.upenn.edu Sent: Tuesday, April 05, 2005 10:07 AM To: Charles.Embrey Cc: Joe Nocito; Chung, Luong; histonet@lists.utsouthwestern.edu Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents I have been watching this debate for over a week now and feel it has added to a good debate and insulted some very good histologist registered, un-registered, with or without degrees and OJT. I am a registered histologist who was trained on the job while in college many years ago. My training was excellent and more research in the beginning than clinical (I have done both). I also spent a number of years in industry and can say I have friends who fall into all categories listed. I know some histologist who were OJT and are better and more knowledgeable than some with degrees and vice versa. The issue we are overlooking is the fact that we have all of these people in Histology and Pathology now and not all have the same opportunities of being in cities or near places with programs. Many hospitals will not pay to send a histologist to a local meeting in town much less state, region or national for more training and to get CEUs. While I know this will fall on some deaf ears not everyone can afford to go and pay out of pocket or for a teleconference when the boss or hospital says NO. This is the real issue and the problem we need to face. The ASCP is our licensing body and yet they have had little interest in where we train or how. It has been to the pathologist advantage to OJT as the salaries could be kept lower and we were not required to have degrees or in some cases register. We do need more education and understanding now as the field has progressed past just special stains and H&Es to immunos. Many who are being trained OJT are not learning basic chemistry of stains but how to order them and who to call in technical service when they fail instead of how to make them. Real training in chemistry and biology in general is needed and we should see what NSH and the ASCP can do to help. If we are not being heard then we need to bring it in louder that is how blood drawing became a field instead of a call for anyone with the touch in the clinical laboratory. Our field is much more complex!! These are critical times and I for one do not want to go back to the old days when IHC first came in and the pathologists wanted to transfer everything to hematology as they were degreed and knfew more. They could not ix tissue and did not understand anything about tissue and most histology laboratories got IHC back within a ew years. Now we could be looking at some of these same ideas again. Don't just fight education help figure out how to make it available to anyone who wants to learn our field and go and recruit at the high schools or community colleges where programs are becoming available. We don't do enough for ourselves to advertise who we are. Off the Soap Box and joining Joe in trouble. Pam Marcum Quoting "Charles.Embrey" : > Joe, Once you finish your OJT requirements, take the certification exam > and become a PA you will see that the OJT vs Program trained debate is > alive and well within the PA ranks. I think many people within the AAPA > and also on histonet fail to realize that OJT stands for On The Job > TRAINING. It is simply another tool to teach a profession. The problem > is that where NAACLS approved programs are all very similar in the > content and level of instruction, OJT programs are not regulated and can > differ greatly. This is where certification helps to level the playing > field. Everyone, program trained or OJT must be able to prove a certain > knowledge base. Even in a very hands on profession as Histology the > required knowledge base of twenty years ago is hardly adequate today. > For the PA exam- Study, study and re-study your Robbins. The knowledge > base is vast and all areas are covered in the exam. I have heard people > complain that they are not good test takers. Well, in my experience, > most people that fail tests just aren't prepared. Degrees are great and > proper OJT is invaluable but even pathologists have to follow their med > school and residency with a very tough board exam. I don't think I want > a pathologist, that failed his boards, reading my slide with the excuse: > "I'm a good pathologist but I failed my boards because I'm not a good > test taker." > I know you and your experience level Joe. Trust me; you will make a > fine and professional (even if a bit vocal) PA. Just remember, > ROBBINS-ROBBINS-ROBBINS. > > Chuck > > > -----Original Message----- > From: Joe Nocito [mailto:jnocito@satx.rr.com] > Sent: Monday, April 04, 2005 8:32 PM > To: Charles.Embrey; Chung, Luong > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: Re: [Histonet] Re: ASCP - My 2 cents > > Okay, > this may be a little out range, but I'm trying to become a > Pathologists' > Assistant doing the OJT route. Does that make me any less professional > that > someone who completes an accredited program? > > Joe Nocito BS, HT(ASCP)QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > ----- Original Message ----- > From: "Charles.Embrey" > To: "Chung, Luong" > Cc: > Sent: Monday, April 04, 2005 1:07 PM > Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents > > > Ok, someone right out of medical school has the M.D. after their name. > Now would you let them remove your gallbladder without assistance before > they do their (OJT)residency in surgery. Sure the degree is about the > knowledge and theory but the OJT is about the practice. I for one think > that a true professional has an ample helping of both. > Chuck > > -----Original Message----- > From: Chung, Luong [mailto:lchung@ppmh.org] > Sent: Monday, April 04, 2005 1:00 PM > To: Charles.Embrey; histonet@lists.utsouthwestern.edu > Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents > > He has an M.D. behind his name. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of > Charles.Embrey > Sent: Monday, April 04, 2005 1:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: FW: Re: [Histonet] Re: ASCP - My 2 cents > > > > > -----Original Message----- > From: Charles.Embrey > Sent: Monday, April 04, 2005 12:56 PM > To: 'Chung, Luong' > Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents > > What do you think a doctor's residency is? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, > Luong > Sent: Monday, April 04, 2005 10:21 AM > To: Larry Woody; Histonet (E-mail) > Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents > > You wouldn't go to see an OJT Doctors or nurses would you? > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry > Woody > Sent: Monday, April 04, 2005 11:03 AM > To: histonet@lists.utsouthwestern.edu > Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents > > > Bravo Ian: The problem here in the US is that there are thousands upon > thousands of people working in histology labs that are not registered > and don't have the theory behind histology and probably never will, and > that is just the way some institutions and organizations want to keep > it. That is not to say that some of these people are not dedicated to > their jobs but if your in the field why not get the registry. Larry > > > --------------------------------- > Do you Yahoo!? > Take Yahoo! Mail with you! Get it on your mobile phone. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Anti-Virus. > Version: 7.0.308 / Virus Database: 266.9.1 - Release Date: 4/1/2005 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Tue Apr 5 11:44:06 2005 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Automation for test 4 cents worth Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328CB82@usca0082k08.labvision.apogent.com> Recent comments on the ASCP test: I personally have a lot of problems with the practical portion where automated machines are allowed to be used to prepare slides. I don't think that I would want to submitt an automated slide. How can you take pride in that?. Well, I have a lot of respect for both Bonnie and Barry, but I'm not sure that what we're testing is whether a tech can successfully answer the timer, drain the slides and put them in the next solution. That is the trivial part of staining. If that's a problem then how about using commercially-prepared stains for the test slides? Should we also require hand-dipping for tissue processing? I think most labs now days both buy the prepared stains and use automation to do the staining. The skill is not the moving of objects through a series of solutions - it is in knowing which to use and how to set up the solutions properly and, most importantly, in identifying when the stain is not correct - and knowing how to correct it. Indeed, even with commercially-prepared stains, there is a lot of skill involved in deciding which one is best for your labs' use. And skill/knowledge certainly is involved in setting up a machine to perform any stain properly - the machine is not magic - it simply does what the user tells it to do. It won't make a bad tech look good simply by its use. Disclaimer - the company I work for manufactures automated instruments for the histology lab. Tim Morken Lab Vision - Neomarkers www.labvision.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie Whitaker Sent: Tuesday, April 05, 2005 9:19 AM To: 'histonet' Subject: RE: [Histonet] 4 cents worth I agree, Barry!!! I don't think that I would want to submitt an automated slide. How can you take pride in that? Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, April 05, 2005 11:05 AM To: histonet Subject: [Histonet] 4 cents worth I must in general agree with Pam and others who have stressed the need for formal recognition of skills such as with a recognized program. I do not think that anyone has been insulted on Histonet with regard to their on the job training. I also had a lot of on the job training. What those of y'all who may be indignant when the quality of OJT is questioned have to realize is that it is not possible to equate OTJ in different workplaces. You may have had superb training or may have been in a job where the training was poor or mediocre at best. You may have been working your buts off or had time to improve your skills and experiment with new procedures. It is difficult to know what your specific situation is unless you have a piece of paper where you may be compared to other individuals. Having passed the ASCP exam does not necessarily mean that you are better trained than somewhat who has worked in a great lab and only received OJT. It does mean that you can be compared to others who have passed the ASCP exam, it is a basic level. Having a degree especially, outside the field may indicate that you have reached a certain level of academia, but also does not guarantee that you have "street smarts" or will be able to pick up techniques and concepts better than an individual who has not received such training. You only have to ask someone who has been placed in charge of helping graduate students with their histology projects to know how true this is. The ASCP exam, while it is not perfect is a first step in ensuring that individuals have the basics. If we are going to work on anything lets work on improving the ASCP examination. I personally have a lot of problems with the practical portion where automated machines are allowed to be used to prepare slides. Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Apr 5 12:02:33 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XT andtheyinsist that we need to use Superfrost Plus s In-Reply-To: Message-ID: <200504051702.j35H2Os0022864@chip.viawest.net> Statlabs has the best price for plus slides I have found. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Tuesday, April 05, 2005 9:10 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XT andtheyinsist that we need to use Superfrost Plus s Angela, You will definitely need "Plus" slides no matter whose instrument you use. Find out which laboratory supply company your institution has a contract with. They may be your least expensive choice. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Tuesday, April 05, 2005 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and theyinsist that we need to use Superfrost Plus s We're talking of buying a Ventana Benchmark XT and they insist that we need to use Superfrost Plus slides. They are going to cost much more than what we're using now. Does anyone have the inside track on the least expensive place to buy them from? You folks are always "The" source for All Histology Wisdom! Thanks! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pex0220 <@t> yahoo.com.cn Tue Apr 5 12:04:46 2005 From: pex0220 <@t> yahoo.com.cn (=?gb2312?q?=CC=EC=20=D0=C1?=) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Immunofluorescence in muscle section Message-ID: <20050405170446.26093.qmail@web15505.mail.cnb.yahoo.com> Hello, all Does someone have any experience about immunofluorescence in muscle section? If yes, I hope that someone can provide a protocol to me, I think that it is not easy. Thank you! Qian --------------------------------- Do You Yahoo!? ×¢²áÊÀ½çÒ»Á÷Æ·ÖʵÄÑÅ»¢Ãâ·ÑµçÓÊ From JWEEMS <@t> sjha.org Tue Apr 5 12:04:50 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist that we need to use Superfrost Plus s Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA45891@sjhaexc02.sjha.org> Mercedes Medical also has good prices. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patsy Ruegg Sent: Tuesday, April 05, 2005 1:03 PM To: 'Sebree Linda A.'; 'Angela Bitting'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist that we need to use Superfrost Plus s Statlabs has the best price for plus slides I have found. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Tuesday, April 05, 2005 9:10 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XT andtheyinsist that we need to use Superfrost Plus s Angela, You will definitely need "Plus" slides no matter whose instrument you use. Find out which laboratory supply company your institution has a contract with. They may be your least expensive choice. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Tuesday, April 05, 2005 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and theyinsist that we need to use Superfrost Plus s We're talking of buying a Ventana Benchmark XT and they insist that we need to use Superfrost Plus slides. They are going to cost much more than what we're using now. Does anyone have the inside track on the least expensive place to buy them from? You folks are always "The" source for All Histology Wisdom! Thanks! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From pmarcum <@t> vet.upenn.edu Tue Apr 5 12:11:13 2005 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XT andtheyinsist that we need to use Superfrost Plus s In-Reply-To: <200504051702.j35H2Os0022864@chip.viawest.net> References: <200504051702.j35H2Os0022864@chip.viawest.net> Message-ID: <1112721073.4252c6b15d0de@imp.vet.upenn.edu> I believe the issue with Ventana saying only Plus slides from Fisher or VWR is that these are all Erie slides and that is the surface that holds best for the Ventana system. You may find slides cheaper however, check to see what the original manufacturer is so the sections stay on the slides better. Pam Marcum Quoting Patsy Ruegg : > Statlabs has the best price for plus slides I have found. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda > A. > Sent: Tuesday, April 05, 2005 9:10 AM > To: Angela Bitting; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XT > andtheyinsist that we need to use Superfrost Plus s > > Angela, > > You will definitely need "Plus" slides no matter whose instrument you use. > Find out which laboratory supply company your institution has a contract > with. They may be your least expensive choice. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela > Bitting > Sent: Tuesday, April 05, 2005 10:31 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and > theyinsist that we need to use Superfrost Plus s > > > We're talking of buying a Ventana Benchmark XT and they insist that we need > to use Superfrost Plus slides. They are going to cost much more than what > we're using now. Does anyone have the inside track on the least expensive > place to buy them from? You folks are always "The" > source for All Histology Wisdom! Thanks! > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. > It is intended solely for the addressee. Access to this message by > anyone else is unauthorized. If you are not the intended recipient, any > disclosure, copying, distribution or any action taken, or omitted to be > taken, in reliance on it is prohibited and may be unlawful. If you have > received this message in error, please delete all electronic copies of > this message (and the documents attached to it, if any), destroy any > hard copies you may have created and notify me immediately by replying > to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From juan.gutierrez <@t> christushealth.org Tue Apr 5 11:52:09 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist that we need to use Superfrost Plus s Message-ID: I believe I may be THE culprit, if not at least one of them that got them to make the request. The problem with using other charged slides is that some of them have more charge on them. The result is that when you have a liquid coverslip and a solution is dropped on the supercharged slide, the solution migrates to the edge of the slide. Needless to say we were getting all this slides with no stain on them. Not even a counterstain was on it. To make a long story longer, I tested the Superfrost Plus slides against my trusty old favorite and Superfrost won:o( Good luck with your search and don't let a little thing like that keep you from buying the best system on the market. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie Whitaker Sent: Tuesday, April 05, 2005 11:24 AM To: histonet@pathology.swmed.edu Subject: FW: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist that we need to use Superfrost Plus s I think the issue is that Ventana is insisting on those specific slides.... Not just any old "Plus Slides". At least that is the issue I face when sending reference work out to a particular laboratory. I have been told that I have to cut them on only Fisher or VWR Superfrost Plus slides because of a problem that exists when using other slides on their particular instrumentation. I HOPE that this issue is being looked at closely!! (Ethel, can you comment here?) Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Tuesday, April 05, 2005 11:10 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XT andtheyinsist that we need to use Superfrost Plus s Angela, You will definitely need "Plus" slides no matter whose instrument you use. Find out which laboratory supply company your institution has a contract with. They may be your least expensive choice. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Tuesday, April 05, 2005 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and theyinsist that we need to use Superfrost Plus s We're talking of buying a Ventana Benchmark XT and they insist that we need to use Superfrost Plus slides. They are going to cost much more than what we're using now. Does anyone have the inside track on the least expensive place to buy them from? You folks are always "The" source for All Histology Wisdom! Thanks! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Tue Apr 5 11:57:10 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Automation for test 4 cents worth Message-ID: When I worked at a histology school here in TX we had our students start from scratch. We also made sure they knew what each reagent's purpose was. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim - Labvision Sent: Tuesday, April 05, 2005 11:44 AM To: 'histonet' Subject: RE: [Histonet] Automation for test 4 cents worth Recent comments on the ASCP test: I personally have a lot of problems with the practical portion where automated machines are allowed to be used to prepare slides. I don't think that I would want to submitt an automated slide. How can you take pride in that?. Well, I have a lot of respect for both Bonnie and Barry, but I'm not sure that what we're testing is whether a tech can successfully answer the timer, drain the slides and put them in the next solution. That is the trivial part of staining. If that's a problem then how about using commercially-prepared stains for the test slides? Should we also require hand-dipping for tissue processing? I think most labs now days both buy the prepared stains and use automation to do the staining. The skill is not the moving of objects through a series of solutions - it is in knowing which to use and how to set up the solutions properly and, most importantly, in identifying when the stain is not correct - and knowing how to correct it. Indeed, even with commercially-prepared stains, there is a lot of skill involved in deciding which one is best for your labs' use. And skill/knowledge certainly is involved in setting up a machine to perform any stain properly - the machine is not magic - it simply does what the user tells it to do. It won't make a bad tech look good simply by its use. Disclaimer - the company I work for manufactures automated instruments for the histology lab. Tim Morken Lab Vision - Neomarkers www.labvision.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie Whitaker Sent: Tuesday, April 05, 2005 9:19 AM To: 'histonet' Subject: RE: [Histonet] 4 cents worth I agree, Barry!!! I don't think that I would want to submitt an automated slide. How can you take pride in that? Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, April 05, 2005 11:05 AM To: histonet Subject: [Histonet] 4 cents worth I must in general agree with Pam and others who have stressed the need for formal recognition of skills such as with a recognized program. I do not think that anyone has been insulted on Histonet with regard to their on the job training. I also had a lot of on the job training. What those of y'all who may be indignant when the quality of OJT is questioned have to realize is that it is not possible to equate OTJ in different workplaces. You may have had superb training or may have been in a job where the training was poor or mediocre at best. You may have been working your buts off or had time to improve your skills and experiment with new procedures. It is difficult to know what your specific situation is unless you have a piece of paper where you may be compared to other individuals. Having passed the ASCP exam does not necessarily mean that you are better trained than somewhat who has worked in a great lab and only received OJT. It does mean that you can be compared to others who have passed the ASCP exam, it is a basic level. Having a degree especially, outside the field may indicate that you have reached a certain level of academia, but also does not guarantee that you have "street smarts" or will be able to pick up techniques and concepts better than an individual who has not received such training. You only have to ask someone who has been placed in charge of helping graduate students with their histology projects to know how true this is. The ASCP exam, while it is not perfect is a first step in ensuring that individuals have the basics. If we are going to work on anything lets work on improving the ASCP examination. I personally have a lot of problems with the practical portion where automated machines are allowed to be used to prepare slides. Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SnyderW <@t> uhcwv.org Tue Apr 5 12:14:13 2005 From: SnyderW <@t> uhcwv.org (Snyder, Wendy) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] CAP negative controls Message-ID: <7B5CC54F6E30BD42A8C61E4896484D19011A9244@uhc-exchange.uhc.wvuhs.com> Hello, I have just received the sept. 04 CAP checklist for AP. There is a revised Immuno question pertaining to negative controls on it. The checklist number is ANP.22570. CAP now requires you to have 2 negative controls. One negative REAGENT control and one negative TISSUE control. I am wanting to put together a negative TISSUE block, a sausage block. I am looking for a list of negative tissue's to use in this block to run on a number of immuno stains. I would love to hear some feed back on this. Can anyone help? Wendy Snyder HT(ASCP) United Hospital Center Clarksburg, WV From Julie.Sanders <@t> med.va.gov Tue Apr 5 12:29:02 2005 From: Julie.Sanders <@t> med.va.gov (Julie.Sanders@med.va.gov) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Superfrost Plus slides Message-ID: <457381D92B01BD44B21CF37CC02EBDFD029275C7@vhacinexc2.v10.med.va.gov> We get ours from Fisher, but I believe they're made by Erie Scientific, you could contact either one for a quote. We get a fairly good price from Fisher. Julie Julie Sanders, Supervisor Anatomic Pathology VAMC, Cincinnati, Ohio on 4-5-05 you wrote: We're talking of buying a Ventana Benchmark XT and they insist that we need to use Superfrost Plus slides. They are going to cost much more than what we're using now. Does anyone have the inside track on the least expensive place to buy them from? You folks are always "The" source for All Histology Wisdom! Thanks! From GDawson <@t> dynacaremilwaukee.com Tue Apr 5 12:27:25 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: ASCP - My 2 cents Message-ID: Great post but it seems odd to me that it should be up to the histotechnician him/herself to advertise for their own field. To tell a histotech that the reason there is a shortage of people in their field is because after putting in a hectic day with possible mandatory overtime, they should be pounding the pavement and heralding not only how great histlogoy is but WHAT histology is seems a bit unfair. That would be a hard row to hoe as well: talk up a field where there are prevalent shortages in the workforce, the payscale is significantly lower than that of other laboratory "professionals", recognition and respect is low, and the number of institutions where you can participate in an accredited course in the field are few and far between. What ever happened to the law of supply and demand? The supply of histotechs is low so the demand and the payscale should be high, right? Sounds good in theory but it's untrue in practice. I can think of many reasons for this but feel no need to be flamed for voicing those ideas. In short, I think that those instututions/individuals that really need histotechs need to be doing the pushing for more of them, not the dwindling pool of existing histotechs. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: pmarcum@vet.upenn.edu [mailto:pmarcum@vet.upenn.edu] Sent: Tuesday, April 05, 2005 9:07 AM To: Charles.Embrey Cc: Joe Nocito; Chung, Luong; histonet@lists.utsouthwestern.edu Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents I have been watching this debate for over a week now and feel it has added to a good debate and insulted some very good histologist registered, un-registered, with or without degrees and OJT. I am a registered histologist who was trained on the job while in college many years ago. My training was excellent and more research in the beginning than clinical (I have done both). I also spent a number of years in industry and can say I have friends who fall into all categories listed. I know some histologist who were OJT and are better and more knowledgeable than some with degrees and vice versa. The issue we are overlooking is the fact that we have all of these people in Histology and Pathology now and not all have the same opportunities of being in cities or near places with programs. Many hospitals will not pay to send a histologist to a local meeting in town much less state, region or national for more training and to get CEUs. While I know this will fall on some deaf ears not everyone can afford to go and pay out of pocket or for a teleconference when the boss or hospital says NO. This is the real issue and the problem we need to face. The ASCP is our licensing body and yet they have had little interest in where we train or how. It has been to the pathologist advantage to OJT as the salaries could be kept lower and we were not required to have degrees or in some cases register. We do need more education and understanding now as the field has progressed past just special stains and H&Es to immunos. Many who are being trained OJT are not learning basic chemistry of stains but how to order them and who to call in technical service when they fail instead of how to make them. Real training in chemistry and biology in general is needed and we should see what NSH and the ASCP can do to help. If we are not being heard then we need to bring it in louder that is how blood drawing became a field instead of a call for anyone with the touch in the clinical laboratory. Our field is much more complex!! These are critical times and I for one do not want to go back to the old days when IHC first came in and the pathologists wanted to transfer everything to hematology as they were degreed and knfew more. They could not ix tissue and did not understand anything about tissue and most histology laboratories got IHC back within a ew years. Now we could be looking at some of these same ideas again. Don't just fight education help figure out how to make it available to anyone who wants to learn our field and go and recruit at the high schools or community colleges where programs are becoming available. We don't do enough for ourselves to advertise who we are. Off the Soap Box and joining Joe in trouble. Pam Marcum Quoting "Charles.Embrey" : > Joe, Once you finish your OJT requirements, take the certification exam > and become a PA you will see that the OJT vs Program trained debate is > alive and well within the PA ranks. I think many people within the AAPA > and also on histonet fail to realize that OJT stands for On The Job > TRAINING. It is simply another tool to teach a profession. The problem > is that where NAACLS approved programs are all very similar in the > content and level of instruction, OJT programs are not regulated and can > differ greatly. This is where certification helps to level the playing > field. Everyone, program trained or OJT must be able to prove a certain > knowledge base. Even in a very hands on profession as Histology the > required knowledge base of twenty years ago is hardly adequate today. > For the PA exam- Study, study and re-study your Robbins. The knowledge > base is vast and all areas are covered in the exam. I have heard people > complain that they are not good test takers. Well, in my experience, > most people that fail tests just aren't prepared. Degrees are great and > proper OJT is invaluable but even pathologists have to follow their med > school and residency with a very tough board exam. I don't think I want > a pathologist, that failed his boards, reading my slide with the excuse: > "I'm a good pathologist but I failed my boards because I'm not a good > test taker." > I know you and your experience level Joe. Trust me; you will make a > fine and professional (even if a bit vocal) PA. Just remember, > ROBBINS-ROBBINS-ROBBINS. > > Chuck > > > -----Original Message----- > From: Joe Nocito [mailto:jnocito@satx.rr.com] > Sent: Monday, April 04, 2005 8:32 PM > To: Charles.Embrey; Chung, Luong > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: Re: [Histonet] Re: ASCP - My 2 cents > > Okay, > this may be a little out range, but I'm trying to become a > Pathologists' > Assistant doing the OJT route. Does that make me any less professional > that > someone who completes an accredited program? > > Joe Nocito BS, HT(ASCP)QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > ----- Original Message ----- > From: "Charles.Embrey" > To: "Chung, Luong" > Cc: > Sent: Monday, April 04, 2005 1:07 PM > Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents > > > Ok, someone right out of medical school has the M.D. after their name. > Now would you let them remove your gallbladder without assistance before > they do their (OJT)residency in surgery. Sure the degree is about the > knowledge and theory but the OJT is about the practice. I for one think > that a true professional has an ample helping of both. > Chuck > > -----Original Message----- > From: Chung, Luong [mailto:lchung@ppmh.org] > Sent: Monday, April 04, 2005 1:00 PM > To: Charles.Embrey; histonet@lists.utsouthwestern.edu > Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents > > He has an M.D. behind his name. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of > Charles.Embrey > Sent: Monday, April 04, 2005 1:56 PM > To: histonet@lists.utsouthwestern.edu > Subject: FW: Re: [Histonet] Re: ASCP - My 2 cents > > > > > -----Original Message----- > From: Charles.Embrey > Sent: Monday, April 04, 2005 12:56 PM > To: 'Chung, Luong' > Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents > > What do you think a doctor's residency is? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, > Luong > Sent: Monday, April 04, 2005 10:21 AM > To: Larry Woody; Histonet (E-mail) > Subject: RE: Re: [Histonet] Re: ASCP - My 2 cents > > You wouldn't go to see an OJT Doctors or nurses would you? > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Larry > Woody > Sent: Monday, April 04, 2005 11:03 AM > To: histonet@lists.utsouthwestern.edu > Subject: Fwd: Re: [Histonet] Re: ASCP - My 2 cents > > > Bravo Ian: The problem here in the US is that there are thousands upon > thousands of people working in histology labs that are not registered > and don't have the theory behind histology and probably never will, and > that is just the way some institutions and organizations want to keep > it. That is not to say that some of these people are not dedicated to > their jobs but if your in the field why not get the registry. Larry > > > --------------------------------- > Do you Yahoo!? > Take Yahoo! Mail with you! Get it on your mobile phone. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Anti-Virus. > Version: 7.0.308 / Virus Database: 266.9.1 - Release Date: 4/1/2005 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sayeed <@t> www.urol.bcm.tmc.edu Tue Apr 5 12:55:57 2005 From: Sayeed <@t> www.urol.bcm.tmc.edu (Sayeed, Mohammed) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] need a good protocol for apoptosis Message-ID: <282891BCC3007E498FAB812EC4BAF7180CE9CF@UROFB-NT.urology.bcm.tmc.local> I need a protocol for apoptosis for paraffin embedded tissue. Has incubating paraffin sections at 50 degrees for a certain length of time any effect on the out come of the apoptosis staining? Thanks. M. Sayeed Histology lab Manager Dept. Of Pathology Prostate research (Spore) Baylor College Of Medicine Phone: 713-798-3650 From bmcmahill <@t> incytepathology.com Tue Apr 5 12:57:10 2005 From: bmcmahill <@t> incytepathology.com (Bonnie J. McMahill) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] CAP negative controls Message-ID: Wendy, We use a multiple tissue control block for all of our immunos. The tissue in it serves the purpose of positive and negative tissue control. We are then able to use it for multiple antibodies. It was fashioned after the "sausage" block that Hector Battifora made many moons ago. Bonnie McMahill Histology Supervisor InCyte Pathology Spokane, WA > -----Original Message----- > From: Snyder, Wendy [SMTP:SnyderW@uhcwv.org] > Sent: Tuesday, April 05, 2005 10:14 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] CAP negative controls > > Hello, > I have just received the sept. 04 CAP checklist for AP. There is a > revised Immuno question pertaining to negative controls on it. The > checklist number is ANP.22570. CAP now requires you to have 2 negative > controls. One negative REAGENT control and one negative TISSUE control. I > am wanting to put together a negative TISSUE block, a sausage block. I am > looking for a list of negative tissue's to use in this block to run on a > number of immuno stains. I would love to hear some feed back on this. Can > anyone help? > > Wendy Snyder HT(ASCP) > United Hospital Center > Clarksburg, WV > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Apr 5 13:50:39 2005 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] need a good protocol for apoptosis In-Reply-To: <282891BCC3007E498FAB812EC4BAF7180CE9CF@UROFB-NT.urology.bcm.tmc.local> Message-ID: <000801c53a10$5cd35a50$76d48a80@AMY> You could look at performing tunel in which you would need to purchase a kit or you could stain immunohistochemically for cleaved caspase 3, which is a marker of apoptosis. The heat should not affect the detection of apoptosis in fixed tissue. To induce apoptosis in cell culture you can place the cells at 36?C for 30 minutes, but you need to control the temp. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sayeed, Mohammed Sent: Tuesday, April 05, 2005 10:56 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] need a good protocol for apoptosis I need a protocol for apoptosis for paraffin embedded tissue. Has incubating paraffin sections at 50 degrees for a certain length of time any effect on the out come of the apoptosis staining? Thanks. M. Sayeed Histology lab Manager Dept. Of Pathology Prostate research (Spore) Baylor College Of Medicine Phone: 713-798-3650 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MadaryJ <@t> MedImmune.com Tue Apr 5 14:11:59 2005 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] automated versus manual for practical(practical testing centers?) Message-ID: <746FDB897740814EA52BDDCB5ED1DDBC1BA4EF@medimmune4.medimmune.com> As most I did all three practicals manually getting the first in 1982. I have to say though as a confirmed cheapskate what of the folks that rely on the lab they are in to do the work and the supervisor will not approve of purchasing reagents for manual, and even if the person paid, would have an issue with the the regs/safety issues of getting new chemicals in the lab? I am all for punishing new people in the lab and making their life difficult espceially those non certified types, oh my God! What if they have no access to manual methods? Maybe the folks using automated methods should have to do more or something. How far does it go, coverslipping? I mean one could argue buying a staining kit is just as bad as automation since you are not` making reagents. I think this is an important issue as you all have pointed out. Personally, I feel there should be testing centers for the practical. Hey there is a money making idea that someone can steal from me! From gcallis <@t> montana.edu Tue Apr 5 14:15:28 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Up to 10 cents worth RE: Automation for test 4 cents worth In-Reply-To: <0556BE8AC5551E4E8AF6BB9E42509BA20328CB82@usca0082k08.labvi sion.apogent.com> References: <0556BE8AC5551E4E8AF6BB9E42509BA20328CB82@usca0082k08.labvision.apogent.com> Message-ID: <6.0.0.22.1.20050405114653.01b504d0@gemini.msu.montana.edu> Tim et al. Couldn't stay out of it, and totally agree with Tim's comments and we don't even have automated IHC in our lab. HOWEVER, if we did have an automated immunostainer and I had to take HT/HTL practical IHC test in this day and age, I would use automated IHC staining. That also goes for using automated microtomes and automated stainers to do the H&E. I personally found HT and HTL practical(s) lessons in discipline - reading up on methods, their theory, setting up and following directions to stain, but more importantly problem solving. It was a learning experience from start to finish both times, and one heck of a review of methods already used in the laboratory. One thing learned, there was no difference between using commecially versus inhouse prepared stains, results were the same - been there, tried and compared (at expense of reagents and time) then using commercial stains for practicals. As for other 2 cents, etc worth commentary on ASCP - I've paid ASCP dues since 1962 and haven't missed a year - ok, so I'm old!! This has never been a sore issue - somehow a necessary maintenance of professional status I paid dues even when there was little money in our pockets, I was not working but lived under a mountain of diapers. Biggest ASCP disappointment was Laboratory Medicine journal - histotechnics became a rare subject but an option to stop subscription was exercised. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman Bozeman MT 59717-3610 At 10:44 AM 4/5/2005, you wrote: >Recent comments on the ASCP test: >I personally have a lot of problems with the practical portion where >automated machines are allowed to be used to prepare slides. >I don't think that I would want to submitt an automated slide. How can you >take pride in that?. > >Well, I have a lot of respect for both Bonnie and Barry, but I'm not sure >that what we're testing is whether a tech can successfully answer the timer, >drain the slides and put them in the next solution. That is the trivial part >of staining. If that's a problem then how about using commercially-prepared >stains for the test slides? Should we also require hand-dipping for tissue >processing? I think most labs now days both buy the prepared stains and use >automation to do the staining. > >The skill is not the moving of objects through a series of solutions - it is >in knowing which to use and how to set up the solutions properly and, most >importantly, in identifying when the stain is not correct - and knowing >how to correct it. Indeed, even with commercially-prepared stains, there is >a lot of skill involved in deciding which one is best for your labs' use. >And skill/knowledge certainly is involved in setting up a machine to perform >any stain properly - the machine is not magic - it simply does what the user >tells it to do. It won't make a bad tech look good simply by its use. > >Disclaimer - the company I work for manufactures automated instruments for >the histology lab. > >Tim Morken >Lab Vision - Neomarkers >www.labvision.com > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie >Whitaker >Sent: Tuesday, April 05, 2005 9:19 AM >To: 'histonet' >Subject: RE: [Histonet] 4 cents worth > > > >I agree, Barry!!! I don't think that I would want to submitt an automated >slide. How can you take pride in that? > >Bonnie Whitaker > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, >Barry R >Sent: Tuesday, April 05, 2005 11:05 AM >To: histonet >Subject: [Histonet] 4 cents worth > > >I must in general agree with Pam and others who have stressed the need for >formal recognition of skills such as with a recognized program. > >I do not think that anyone has been insulted on Histonet with regard to >their on the job training. I also had a lot of on the job training. What >those of y'all who may be indignant when the quality of OJT is questioned >have to realize is that it is not possible to equate OTJ in different >workplaces. > >You may have had superb training or may have been in a job where the >training was poor or mediocre at best. You may have been working your buts >off or had time to improve your skills and experiment with new procedures. >It is difficult to know what your specific situation is unless you have a >piece of paper where you may be compared to other individuals. Having passed >the ASCP exam does not necessarily mean that you are better trained than >somewhat who has worked in a great lab and only received OJT. It does mean >that you can be compared to others who have passed the ASCP exam, it is a >basic level. > >Having a degree especially, outside the field may indicate that you have >reached a certain level of academia, but also does not guarantee that you >have "street smarts" or will be able to pick up techniques and concepts >better than an individual who has not received such training. You only have >to ask someone who has been placed in charge of helping graduate students >with their histology projects to know how true this is. > >The ASCP exam, while it is not perfect is a first step in ensuring that >individuals have the basics. > >If we are going to work on anything lets work on improving the ASCP >examination. > >I personally have a lot of problems with the practical portion where >automated machines are allowed to be used to prepare slides. > >Barry > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters <@t> uiowa.edu Tue Apr 5 14:17:50 2005 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] bacteria stain in plant tissue Message-ID: Hi, Has anyone had any experience staining for bacteria in plant tissues? In Ruzin's book basic fuchsin is used, but it also stains nuclei, mucin and lignified tissues. My bacteria of interest is gram negative. Thanks for any ideas you can give me!! Kathy From millicent_renee <@t> yahoo.com Tue Apr 5 14:24:58 2005 From: millicent_renee <@t> yahoo.com (Millicent Bruce) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] time on mart-1 stain Message-ID: <20050405192458.93626.qmail@web31508.mail.mud.yahoo.com> Hello. I posted a question a couple days ago about information on the mart-1 stain. Thank you for all the information. My next question is: The procedure I got takes about 2 hours. The surgeon said he heard that it has been able to be cut down to 30 mintues to an hour. Has or does anybody know about the shorter process? Thanks for the help, Millicent Bruce __________________________________ Do you Yahoo!? Yahoo! Personals - Better first dates. More second dates. http://personals.yahoo.com From cfavara <@t> niaid.nih.gov Tue Apr 5 14:33:10 2005 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] bacteria stain in plant tissue Message-ID: I have no experience with plant tissue so this is just a thought! Have you tried just a plan gram Stain? Others would be Brown-Hopps and Gram Twort. I like both of these in tissue. Good Luck! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Walters, Katherine S [mailto:katherine-walters@uiowa.edu] Sent: Tuesday, April 05, 2005 12:18 PM To: histonet@pathology.swmed.edu Subject: [Histonet] bacteria stain in plant tissue Hi, Has anyone had any experience staining for bacteria in plant tissues? In Ruzin's book basic fuchsin is used, but it also stains nuclei, mucin and lignified tissues. My bacteria of interest is gram negative. Thanks for any ideas you can give me!! Kathy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maxim_71 <@t> mail.ru Tue Apr 5 14:11:13 2005 From: maxim_71 <@t> mail.ru (Maxim) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Re: Good procedure for Cat Scratch Fever Message-ID: <199504718.20050405231113@mail.ru> Hi, Karen! I looking this procedure already much years. For determination of the Chlamydia I use parallel stains Gram, PAS, AFB for FFPE sections of lymph nodes. This organisms are PAS-positive, gramnegative, not stainable carbol-fuchsine. I don't have possibles making ISH and IHC techniques. This organisms are seen into macrophages and endothelial cells as PAS-positive granules of varuios sizes. And me helped specific morphologic picture and clinical data. And I use methods Warthin-Staryy (AFIP modification, 1994). Hope this help. Maxim Peshkov, HTL. Department of biopsy and cytological researches. Pathological and anatomical bureau. Russia, Taganrog mailto:maxim_71@mail.ru From Barry.R.Rittman <@t> uth.tmc.edu Tue Apr 5 14:39:49 2005 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Sep 16 15:24:54 2005 Subject: [Histonet] Up to 10 cents worth RE: Automation for test 4 centsworth Message-ID: <566FB0B522443D43AF02D2ADBE35A6F00163F9BC@UTHEVS3.mail.uthouston.edu> I think that an important point has been lost here. It makes no difference if you make up your own solutions or use commercially available ones if the results are the same. In England where I trained cost was a definite factor. I would happily have mowed lawns for a week rather than make up the Feulgen reagent again, it was a real pain, and commercial ones now seem to be very good. One positive aspect of making solutions is that it forces one to understand the ingredients and manual staining the method and therefore able to make corrections if anything goes wrong. It is often this lack of understanding of the methods used that is distressing to me. It is the same with machines, is the staining carried out by the histotech in a robotic manner? If you are going to use machine staining then the basis for that staining needs to be clearly understood. I agree that taking courses and exams even if you feel that you are an expert is valuable as it often forces one to look from a different perspective (and sometimes provides that necessary humbling). Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, April 05, 2005 2:15 PM To: Morken, Tim - Labvision; Histonet@lists.utsouthwestern.edu Subject: [Histonet] Up to 10 cents worth RE: Automation for test 4 centsworth Tim et al. Couldn't stay out of it, and totally agree with Tim's comments and we don't even have automated IHC in our lab. HOWEVER, if we did have an automated immunostainer and I had to take HT/HTL practical IHC test in this day and age, I would use automated IHC staining. That also goes for using automated microtomes and automated stainers to do the H&E. I personally found HT and HTL practical(s) lessons in discipline - reading up on methods, their theory, setting up and following directions to stain, but more importantly problem solving. It was a learning experience from start to finish both times, and one heck of a review of methods already used in the laboratory. One thing learned, there was no difference between using commecially versus inhouse prepared stains, results were the same - been there, tried and compared (at expense of reagents and time) then using commercial stains for practicals. As for other 2 cents, etc worth commentary on ASCP - I've paid ASCP dues since 1962 and haven't missed a year - ok, so I'm old!! This has never been a sore issue - somehow a necessary maintenance of professional status I paid dues even when there was little money in our pockets, I was not working but lived under a mountain of diapers. Biggest ASCP disappointment was Laboratory Medicine journal - histotechnics became a rare subject but an option to stop subscription was exercised. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman Bozeman MT 59717-3610 At 10:44 AM 4/5/2005, you wrote: >Recent comments on the ASCP test: >I personally have a lot of problems with the practical portion where >automated machines are allowed to be used to prepare slides. >I don't think that I would want to submitt an automated slide. How can you >take pride in that?. > >Well, I have a lot of respect for both Bonnie and Barry, but I'm not sure >that what we're testing is whether a tech can successfully answer the timer, >drain the slides and put them in the next solution. That is the trivial part >of staining. If that's a problem then how about using commercially-prepared >stains for the test slides? Should we also require hand-dipping for tissue >processing? I think most labs now days both buy the prepared stains and use >automation to do the staining. > >The skill is not the moving of objects through a series of solutions - it is >in knowing which to use and how to set up the solutions properly and, most >importantly, in identifying when the stain is not correct - and knowing >how to correct it. Indeed, even with commercially-prepared stains, there is >a lot of skill involved in deciding which one is best for your labs' use. >And skill/knowledge certainly is involved in setting up a machine to perform >any stain properly - the machine is not magic - it simply does what the user >tells it to do. It won't make a bad tech look good simply by its use. > >Disclaimer - the company I work for manufactures automated instruments for >the histology lab. > >Tim Morken >Lab Vision - Neomarkers >www.labvision.com > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie >Whitaker >Sent: Tuesday, April 05, 2005 9:19 AM >To: 'histonet' >Subject: RE: [Histonet] 4 cents worth > > > >I agree, Barry!!! I don't think that I would want to submitt an automated >slide. How can you take pride in that? > >Bonnie Whitaker > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, >Barry R >Sent: Tuesday, April 05, 2005 11:05 AM >To: histonet >Subject: [Histonet] 4 cents worth > > >I must in general agree with Pam and others who have stressed the need for >formal recognition of skills such as with a recognized program. > >I do not think that anyone has been insulted on Histonet with regard to >their on the job training. I also had a lot of on the job training. What >those of y'all who may be indignant when the quality of OJT is questioned >have to realize is that it is not possible to equate OTJ in different >workplaces. > >You may have had superb training or may have been in a job where the >training was poor or mediocre at best. You may have been working your buts >off or had time to improve your skills and experiment with new procedures. >It is difficult to know what your specific situation is unless you have a >piece of paper where you may be compared to other individuals. Having passed >the ASCP exam does not necessarily mean that you are better trained than >somewhat who has worked in a great lab and only received OJT. It does mean >that you can be compared to others who have passed the ASCP exam, it is a >basic level. > >Having a degree especially, outside the field may indicate that you have >reached a certain level of academia, but also does not guarantee that you >have "street smarts" or will be able to pick up techniques and concepts >better than an individual who has not received such training. You only have >to ask someone who has been placed in charge of helping graduate students >with their histology projects to know how true this is. > >The ASCP exam, while it is not perfect is a first step in ensuring that >individuals have the basics. > >If we are going to work on anything lets work on improving the ASCP >examination. > >I personally have a lot of problems with the practical portion where >automated machines are allowed to be used to prepare slides. > >Barry > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Tue Apr 5 14:42:36 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] automated versus manual for practical(practical testingcenters?) Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA45898@sjhaexc02.sjha.org> Perhaps its time to stop the practical - do the other disciplines do a practical? It's all so subjective. I think our problem as a laboratory science is that we do not make diagnoses - that sets us apart from the others. And some pathologists have promoted the separation by not respecting us for how important we are to them. My belief is that a pathologist is only as good as the tech. Another 2 cents worth. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Madary, Joseph Sent: Tuesday, April 05, 2005 3:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automated versus manual for practical(practical testingcenters?) As most I did all three practicals manually getting the first in 1982. I have to say though as a confirmed cheapskate what of the folks that rely on the lab they are in to do the work and the supervisor will not approve of purchasing reagents for manual, and even if the person paid, would have an issue with the the regs/safety issues of getting new chemicals in the lab? I am all for punishing new people in the lab and making their life difficult espceially those non certified types, oh my God! What if they have no access to manual methods? Maybe the folks using automated methods should have to do more or something. How far does it go, coverslipping? I mean one could argue buying a staining kit is just as bad as automation since you are not` making reagents. I think this is an important issue as you all have pointed out. Personally, I feel there should be testing centers for the practical. Hey there is a money making idea that someone can steal from me! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From dmccaig <@t> ckha.on.ca Tue Apr 5 14:45:31 2005 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] INKING BREAST SPECIMENS Message-ID: <3E5A3F039F0BD8118B4700C00D00202404352E@CKHA9> Is there a universal protocol for colors when inking mastectomy specimens? Are green, blue, yellow, red and black assigned to a particular aspect of the specimen or is it pathologists preference? Thanks Diana From djohnson14 <@t> hotmail.com Tue Apr 5 15:21:05 2005 From: djohnson14 <@t> hotmail.com (Dave Johnson) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Superfrost Plus slides In-Reply-To: <457381D92B01BD44B21CF37CC02EBDFD029275C7@vhacinexc2.v10.med.va.gov> Message-ID: Anyone know why only the Erie slides work on the Ventana? >From: Julie.Sanders@med.va.gov >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Superfrost Plus slides >Date: Tue, 5 Apr 2005 12:29:02 -0500 > > >We get ours from Fisher, but I believe they're made by Erie Scientific, you >could contact either one for a quote. We get a fairly good price from >Fisher. >Julie >Julie Sanders, Supervisor Anatomic Pathology >VAMC, Cincinnati, Ohio > > > >on 4-5-05 you wrote: >We're talking of buying a Ventana Benchmark XT and they insist that we >need to use Superfrost Plus slides. They are going to cost much more >than what we're using now. Does anyone have the inside track on the >least expensive place to buy them from? >You folks are always "The" source for All Histology Wisdom! >Thanks! > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bjrenquist <@t> ucdavis.edu Tue Apr 5 15:29:38 2005 From: bjrenquist <@t> ucdavis.edu (Ben) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Cryosectioning cryoprotected (30% sucrose) brain Message-ID: <200504052029.j35KTXGS013331@pop19.ucdavis.edu> Hello All, I am attempting to slice sheep brainstem and hypothalamus on a cryostat at -20 C. The tissue was treated as such: Perfusion through carotid artery (6L of 4% paraformaldehyde) Postfixation for 24 hours at 4 C Cryoprotected in a 20% sucrose solution for 5 days at 4 C Cryoprotected in a 30% sucrose 30% ethylene glycol solution and stored at -20 C. I am having some major problems with slicing this tissue. It appears to never freeze. Does anyone know at what temperature I should be slicing? Any opinions on use of a vibratome instead of a cryostat? I'm trying to section tissue at 30-40 um. Thanks in advance for your help. Ben Renquist UCDavis From DDDeltour <@t> mar.med.navy.mil Tue Apr 5 16:00:27 2005 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Superfrost Plus slides BENCHMARK EXPLANATION Message-ID: <080566D001A3D9459FFC0A391A646C9104BEF8AC@marxchg03.mar.med.navy.mil> If you are talking about the slides that have red control border it is because of the paint. The painted bordr on the Erie slides is painted on the back of the slide, not the top. The Benchmark was having problems with reagent pooling and not rinsing because of this "built-up" paint border. Here is the fisher site that has the slides. https://www1.fishersci.com/Coupon?cid=1333&gid=45852 Notice how they are listed for automatic stainers and manual staining. Doug -----Original Message----- From: Dave Johnson To: histonet@lists.utsouthwestern.edu Sent: 4/5/2005 4:21 PM Subject: RE: [Histonet] Superfrost Plus slides Anyone know why only the Erie slides work on the Ventana? >From: Julie.Sanders@med.va.gov >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Superfrost Plus slides >Date: Tue, 5 Apr 2005 12:29:02 -0500 > > >We get ours from Fisher, but I believe they're made by Erie Scientific, you >could contact either one for a quote. We get a fairly good price from >Fisher. >Julie >Julie Sanders, Supervisor Anatomic Pathology >VAMC, Cincinnati, Ohio > > > >on 4-5-05 you wrote: >We're talking of buying a Ventana Benchmark XT and they insist that we >need to use Superfrost Plus slides. They are going to cost much more >than what we're using now. Does anyone have the inside track on the >least expensive place to buy them from? >You folks are always "The" source for All Histology Wisdom! >Thanks! > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mainlinemohs <@t> yahoo.com Tue Apr 5 16:12:47 2005 From: mainlinemohs <@t> yahoo.com (Michael Lehrer) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] time on mart-1 stain In-Reply-To: 6667 Message-ID: <20050405211247.98267.qmail@web31101.mail.mud.yahoo.com> We're at 90 min to 2 hours and can't find any steps to eliminate. I'm not sure how they claim this can be done faster, but I welcome any suggestions. Michael S. Lehrer, MD Millicent Bruce wrote: Hello. I posted a question a couple days ago about information on the mart-1 stain. Thank you for all the information. My next question is: The procedure I got takes about 2 hours. The surgeon said he heard that it has been able to be cut down to 30 mintues to an hour. Has or does anybody know about the shorter process? Thanks for the help, Millicent Bruce __________________________________ Do you Yahoo!? Yahoo! Personals - Better first dates. More second dates. http://personals.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Yahoo! Mail - Helps protect you from nasty viruses. From bills <@t> icpmr.wsahs.nsw.gov.au Tue Apr 5 16:14:34 2005 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] automated versus manual for practical(practicaltestingcenters?) In-Reply-To: <83AACDB0810528418AA106F9AE9B7F7EA45898@wsahs.nsw.gov.au> Message-ID: <001501c53a24$775fd510$0ecd080a@wsahs.nsw.gov.au> Dear Histonetters, On of my mentors and probably the Pathologist I learn most pathology from always said we work in Histopathology. "Histo" referring to the histotechs function while "pathology" referred to the Pathologist function, a team effort? Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Wednesday, 06 April 2005 5:43 AM To: Madary, Joseph; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] automated versus manual for practical(practicaltestingcenters?) Perhaps its time to stop the practical - do the other disciplines do a practical? It's all so subjective. I think our problem as a laboratory science is that we do not make diagnoses - that sets us apart from the others. And some pathologists have promoted the separation by not respecting us for how important we are to them. My belief is that a pathologist is only as good as the tech. Another 2 cents worth. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Madary, Joseph Sent: Tuesday, April 05, 2005 3:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automated versus manual for practical(practical testingcenters?) As most I did all three practicals manually getting the first in 1982. I have to say though as a confirmed cheapskate what of the folks that rely on the lab they are in to do the work and the supervisor will not approve of purchasing reagents for manual, and even if the person paid, would have an issue with the the regs/safety issues of getting new chemicals in the lab? I am all for punishing new people in the lab and making their life difficult espceially those non certified types, oh my God! What if they have no access to manual methods? Maybe the folks using automated methods should have to do more or something. How far does it go, coverslipping? I mean one could argue buying a staining kit is just as bad as automation since you are not` making reagents. I think this is an important issue as you all have pointed out. Personally, I feel there should be testing centers for the practical. Hey there is a money making idea that someone can steal from me! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From petepath <@t> yahoo.com Tue Apr 5 16:15:22 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] re:INKING BREAST SPECIMENS Message-ID: <20050405211522.90198.qmail@web30412.mail.mud.yahoo.com> Hi Diana, As far as I know there is no universal protocol. As a pathologist I can tell you it is very useful to have a standard in your lab so the pathologist does not have to keep referring to the gross for every case and slide. I instituted a protocol in our lab which I will share with you. It is a bit silly but here is a way to remember it. Red is a Superior color Yellow is an Inferior color ( also we P-- downward) Green has an E like Medial Blue is and should be opposite Green and therefore Lateral Black is on the back Posterior Orange a pregnant woman has a pumpkin in the front Anterior Now when I see a slide with yellow I know its inferior and black I know is posterior. I do not have to look up how every slide is labled and inked. It makes life much easier. This arrangement takes into account that green and blue can look similar on the gross tissue and therefore should not be right next to each other. The only time we deviate from this is if the anatomy of the specimen requires a different scheme such as in skin lesion from a right or left side. Otherwise our residents and PA's dictate" inked according to departmental protocol." Another obsevation to pass to your pathologists. Sometimes yellow and orange can look similar under the microscope. If they cannot tell them apart, if you polarize the tissue the orange ink polarizes orange yellow color and the yellow ink polarizes a yellow green color. Stephen Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 From gcallis <@t> montana.edu Tue Apr 5 16:52:51 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Cryosectioning cryoprotected (30% sucrose) brain In-Reply-To: <200504052029.j35KTXGS013331@pop19.ucdavis.edu> References: <200504052029.j35KTXGS013331@pop19.ucdavis.edu> Message-ID: <6.0.0.22.1.20050405154617.01b18858@gemini.msu.montana.edu> Ethylene glycol is anti-freeze - don't use it. Just let brain cryoprotect in 30% sucrose - we never bother with a sucrose gradient anymore but you can use it if you want. You should snap freeze the brain properly and then get good frozen sections at that thickness at approx -17C. but if you have anti-freeze in tissue, you are probably cutting mush at that temperature. At 02:29 PM 4/5/2005, you wrote: >Hello All, > > > >I am attempting to slice sheep brainstem and hypothalamus on a cryostat at >-20 C. The tissue was treated as such: > > > >Perfusion through carotid artery (6L of 4% paraformaldehyde) > >Postfixation for 24 hours at 4 C > >Cryoprotected in a 20% sucrose solution for 5 days at 4 C > >Cryoprotected in a 30% sucrose 30% ethylene glycol solution and stored at >-20 C. > > > >I am having some major problems with slicing this tissue. It appears to >never freeze. Does anyone know at what temperature I should be slicing? >Any opinions on use of a vibratome instead of a cryostat? I'm trying to >section tissue at 30-40 um. Thanks in advance for your help. > > > >Ben Renquist > >UCDavis > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From AnthonyH <@t> chw.edu.au Tue Apr 5 16:59:59 2005 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] nails[Scanned] Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3741269E214@simba.kids> Perming solution (Ammonium thiglycolate?) does soften the nail, but stinks. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Wednesday, 6 April 2005 1:23 AM To: Kemlo Rogerson; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] What makes clothes soft doesn't necessarily make nails soft. Fabric conditioners work by fluffing out fibres, or at least, stopping them matting. There be no fibres in toe nails (unless you have a fungal infection). Some swear by decalcifying fluid, though there be no calcium in nails either. But, there be water in decalcifying fluid. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 05 April 2005 16:06 To: Marshall Terry Dr, Consultant Histopathologist; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] Don't think it's just water, I assuming it's something dead scientific that alters the chemical bonding between proteins; like hydrolysis or something. I mean what does Fabric conditioner have in it that makes cloths soft? No I think it's something dead scientific, but I'll be beggared if I know. -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 05 April 2005 16:00 To: Kemlo Rogerson; Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] This business of softening nails continues to intrigue me. It seems that the only common factor (in all the various protocols) is water, and that what you are doing is hydrating the nail. Of course, you then process and dehydrate it. It remains a mystery. I remain unconvinced at the utility of any method. I our hands, nails stay as hard as - well - nails I guess. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 05 April 2005 15:49 To: 'Dorothy.L.Webb@HealthPartners.Com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] nails[Scanned] Fabric softener apparently does it to. Dunno which one, but it apparently works; wonders if its got Tween in it too? -----Original Message----- From: Dorothy.L.Webb@HealthPartners.Com [mailto:Dorothy.L.Webb@HealthPartners.Com] Sent: 05 April 2005 15:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] nails[Scanned] There is a product from Sigma called "Tween" that works great at both softening and fixation. It is a 5% solution in 10% formalin and stays in there for 24 hours and then processed routinely, cut and placed on a charged slide and we only stain for PAS unless it has the nailbed with it. The order number is P4634. Good luck! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From djohnson14 <@t> hotmail.com Tue Apr 5 17:14:49 2005 From: djohnson14 <@t> hotmail.com (Dave Johnson) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Superfrost Plus slides BENCHMARK EXPLANATION In-Reply-To: <080566D001A3D9459FFC0A391A646C9104BEF8AC@marxchg03.mar.med.navy.mil> Message-ID: I was told that the Mercedes Medical and Surgipath plus slides dont work for whatever reason but the Erie ones do. What is the difference in the coating? Silane Poly lysine >From: "Deltour, Douglas D. (HM2)" >To: 'Dave Johnson ' , >"'histonet@lists.utsouthwestern.edu '" >Subject: RE: [Histonet] Superfrost Plus slides BENCHMARK EXPLANATION >Date: Tue, 5 Apr 2005 17:00:27 -0400 > > If you are talking about the slides that have red control border it is >because of the paint. The painted bordr on the Erie slides is painted on >the >back of the slide, not the top. The Benchmark was having problems with >reagent pooling and not rinsing because of this "built-up" paint border. >Here is the fisher site that has the slides. >https://www1.fishersci.com/Coupon?cid=1333&gid=45852 >Notice how they are listed for automatic stainers and manual staining. > >Doug > >-----Original Message----- >From: Dave Johnson >To: histonet@lists.utsouthwestern.edu >Sent: 4/5/2005 4:21 PM >Subject: RE: [Histonet] Superfrost Plus slides > > >Anyone know why only the Erie slides work on the Ventana? > > > >From: Julie.Sanders@med.va.gov > >To: histonet@lists.utsouthwestern.edu > >Subject: [Histonet] Superfrost Plus slides > >Date: Tue, 5 Apr 2005 12:29:02 -0500 > > > > > >We get ours from Fisher, but I believe they're made by Erie Scientific, >you > >could contact either one for a quote. We get a fairly good price from > >Fisher. > >Julie > >Julie Sanders, Supervisor Anatomic Pathology > >VAMC, Cincinnati, Ohio > > > > > > > >on 4-5-05 you wrote: > >We're talking of buying a Ventana Benchmark XT and they insist that we > >need to use Superfrost Plus slides. They are going to cost much more > >than what we're using now. Does anyone have the inside track on the > >least expensive place to buy them from? > >You folks are always "The" source for All Histology Wisdom! > >Thanks! > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From conniegrubaugh <@t> hotmail.com Tue Apr 5 17:54:16 2005 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] automated versus manual for practical(practicaltestingcenters?) In-Reply-To: <83AACDB0810528418AA106F9AE9B7F7EA45898@sjhaexc02.sjha.org> Message-ID: I think it is past time to stop the practical. So many of the stains are done on machines. I have had a " registered tech," that did not know how to run slides manually to just de-parffinize them. Why can't there just be more questions on the exam of theory ect. ect. ect. And that is my 2 cents worth....... Connie G. >From: "Weems, Joyce" >To: "Madary, Joseph" , >Subject: RE: [Histonet] automated versus manual for practical(practicaltestingcenters?) >Date: Tue, 5 Apr 2005 15:42:36 -0400 > >Perhaps its time to stop the practical - do the other disciplines do a practical? It's all so subjective. I think our problem as a laboratory science is that we do not make diagnoses - that sets us apart from the others. And some pathologists have promoted the separation by not respecting us for how important we are to them. My belief is that a pathologist is only as good as the tech. Another 2 cents worth. > >Joyce > >Joyce Weems >Pathology Manager >Saint Joseph's Hospital >5665 Peachtree Dunwoody Rd NE >Atlanta, GA 30342 >404-851-7376 - Phone >404-851-7831 - Fax > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Madary, >Joseph >Sent: Tuesday, April 05, 2005 3:12 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] automated versus manual for practical(practical >testingcenters?) > > >As most I did all three practicals manually getting the first in 1982. I have to say though as a confirmed cheapskate what of the folks that rely on the lab they are in to do the work and the supervisor will not approve of purchasing reagents for manual, and even if the person paid, would have an issue with the the regs/safety issues of getting new chemicals in the lab? I am all for punishing new people in the lab and making their life difficult espceially those non certified types, oh my God! What if they have no access to manual methods? Maybe the folks using automated methods should have to do more or something. How far does it go, coverslipping? I mean one could argue buying a staining kit is just as bad as automation since you are not` making reagents. I think this is an important issue as you all have pointed out. Personally, I feel there should be testing centers for the practical. Hey there is a money making idea that someone can steal from me! > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. >Thank you. Saint Josephs Health System, Inc. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lindas <@t> awesomenet.net Tue Apr 5 18:00:29 2005 From: lindas <@t> awesomenet.net (Linda Davis) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] antibody References: Message-ID: <00ac01c53a33$43835230$b50ac942@D6JLZ851> Try Esoterix or Quest Diagnostics. Linda Davis ----- Original Message ----- From: To: Sent: Tuesday, April 05, 2005 10:31 AM Subject: [Histonet] antibody > > Does anyone know who performs IHC testing for rickettsieae..we checked > with > Mayo and Propath..any ideas and thanks!! > > > ________________________________________ > > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please > be advised that you have received this e-mail in error and that any > use, dissemination, forwarding, printing, or copying of this e-mail > is strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. > You will be reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > No virus found in this incoming message. > Checked by AVG Anti-Virus. > Version: 7.0.308 / Virus Database: 266.9.1 - Release Date: 4/1/2005 > > -- No virus found in this outgoing message. Checked by AVG Anti-Virus. Version: 7.0.308 / Virus Database: 266.9.1 - Release Date: 4/1/2005 From jnocito <@t> satx.rr.com Tue Apr 5 18:52:55 2005 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] INKING BREAST SPECIMENS References: <3E5A3F039F0BD8118B4700C00D00202404352E@CKHA9> Message-ID: <003a01c53a3a$97292f20$7929f318@yourxhtr8hvc4p> Diana, we just use one color, but we separate the four quadrants and dictate which quadrant is placed in which cassettes. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Diana McCaig" To: Sent: Tuesday, April 05, 2005 2:45 PM Subject: [Histonet] INKING BREAST SPECIMENS > Is there a universal protocol for colors when inking mastectomy > specimens? > Are green, blue, yellow, red and black assigned to a particular aspect of > the specimen or is it pathologists preference? > Thanks > Diana > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Anti-Virus. > Version: 7.0.308 / Virus Database: 266.9.3 - Release Date: 4/5/2005 > > From jmahoney <@t> alegent.org Tue Apr 5 18:54:48 2005 From: jmahoney <@t> alegent.org (Janice A Mahoney) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Cyto net Message-ID: Can anyone tell me if there is a "cytonet" for cytology like this one for histology? Thanks, Jan From jwatson <@t> gnf.org Tue Apr 5 20:32:19 2005 From: jwatson <@t> gnf.org (James Watson) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Cryosectioning cryoprotected (30% sucrose) brain Message-ID: Ben, The only time I have seen or used the ethylene glycol in sucrose as a cryoprotectant has been on brains that have been cut on a sliding microtome (using dry ice to freeze the tissue), for thick sections (30 microns +), for floating sections. The sections collapse on the knife, are picked up with a paint brush, and flattened out in PBS in the well plate for staining before mounting onto the slide. James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, April 05, 2005 2:53 PM To: Ben; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cryosectioning cryoprotected (30% sucrose) brain Ethylene glycol is anti-freeze - don't use it. Just let brain cryoprotect in 30% sucrose - we never bother with a sucrose gradient anymore but you can use it if you want. You should snap freeze the brain properly and then get good frozen sections at that thickness at approx -17C. but if you have anti-freeze in tissue, you are probably cutting mush at that temperature. At 02:29 PM 4/5/2005, you wrote: >Hello All, > > > >I am attempting to slice sheep brainstem and hypothalamus on a cryostat >at -20 C. The tissue was treated as such: > > > >Perfusion through carotid artery (6L of 4% paraformaldehyde) > >Postfixation for 24 hours at 4 C > >Cryoprotected in a 20% sucrose solution for 5 days at 4 C > >Cryoprotected in a 30% sucrose 30% ethylene glycol solution and stored >at -20 C. > > > >I am having some major problems with slicing this tissue. It appears >to never freeze. Does anyone know at what temperature I should be >slicing? Any opinions on use of a vibratome instead of a cryostat? I'm trying to >section tissue at 30-40 um. Thanks in advance for your help. > > > >Ben Renquist > >UCDavis > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From herme013 <@t> umn.edu Tue Apr 5 22:22:48 2005 From: herme013 <@t> umn.edu (Yves Heremans) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] zincTris fixation on frozen sections Message-ID: <6a48b3695164cba5657f40248038766e@umn.edu> Dear All, I am in the process of staining mouse kidney samples containing grafted mouse pancreatic islets with antibodies against MHC H2Dd and H2Db. Tissue samples available are either FFPE or unfixed, snap frozen. I decided to concentrate on the frozen samples due to the general difficulty of staining for surface markers after formalin fixation. My questions are: 1. If I want to use frozen sections, immediately fixed in zincTris (formalin-free), will I need to process the slides through increasing alcohols just as suggested for zincTris fixation of tissue samples ? 2. Will zincTris fixation yield better morphology compared to conventional acetone fixation ? Yves Heremans University of Minnesota Stem Cell Institute Tel 612-625-0964 Fax 612-624-2436 Address for US Postal Mail: University of Minnesota MMC 716 420 Delaware Street SE Minneapolis, MN 55455 Address for COURIER DELIVERY: Suite 14-285 Moos Tower 515 Delaware Street SE From Kemlo.Rogerson <@t> elht.nhs.uk Wed Apr 6 02:32:47 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Cyto net[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F14B@bhrv-nt-11.bhrv.nwest.nhs.uk> There was but it seems to have disappeared. I posted a thread a few months back, got some very odd replies. It was Cytopathnet.org -----Original Message----- From: Janice A Mahoney [mailto:jmahoney@alegent.org] Sent: 06 April 2005 00:55 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cyto net[Scanned] Can anyone tell me if there is a "cytonet" for cytology like this one for histology? Thanks, Jan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgfields <@t> lexhealth.org Wed Apr 6 07:35:56 2005 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Superfrost Plus slides Message-ID: Erie makes all the slides made.... at least this is what I have been told for years. I am not sure if each company coats their own or Erie does that also. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Dave Johnson [mailto:djohnson14@hotmail.com] Sent: Tuesday, April 05, 2005 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Superfrost Plus slides Anyone know why only the Erie slides work on the Ventana? >From: Julie.Sanders@med.va.gov >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Superfrost Plus slides >Date: Tue, 5 Apr 2005 12:29:02 -0500 > > >We get ours from Fisher, but I believe they're made by Erie Scientific, you >could contact either one for a quote. We get a fairly good price from >Fisher. >Julie >Julie Sanders, Supervisor Anatomic Pathology >VAMC, Cincinnati, Ohio > > > >on 4-5-05 you wrote: >We're talking of buying a Ventana Benchmark XT and they insist that we >need to use Superfrost Plus slides. They are going to cost much more >than what we're using now. Does anyone have the inside track on the >least expensive place to buy them from? >You folks are always "The" source for All Histology Wisdom! >Thanks! > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From Sayeed <@t> www.urol.bcm.tmc.edu Wed Apr 6 07:56:23 2005 From: Sayeed <@t> www.urol.bcm.tmc.edu (Sayeed, Mohammed) Date: Fri Sep 16 15:24:55 2005 Subject: FW: [Histonet] need a good protocol for apoptosis Message-ID: <282891BCC3007E498FAB812EC4BAF7180CE9D8@UROFB-NT.urology.bcm.tmc.local> Dr Ayala wrote. M. Sayeed Histology lab Manager Dept. Of Pathology Prostate research (Spore) Baylor College Of Medicine Phone: 713-798-3650 -----Original Message----- From: Gustavo Ayala [mailto:gayala@bcm.tmc.edu] Sent: Tuesday, April 05, 2005 3:47 PM To: Sayeed, Mohammed Subject: Re: [Histonet] need a good protocol for apoptosis We are doing tunnel. We have done caspase 3 and it does not correlate with tunnel. GA On Apr 5, 2005, at 2:04 PM, Sayeed, Mohammed wrote: d a good protocol for apoptosis You could look at performing tunel in which you would need to purchase a kit or you could stain immunohistochemically for cleaved caspase 3, which is a marker of apoptosis. The heat should not affect the detection of apoptosis in fixed tissue. To induce apoptosis in cell culture you can place the cells at 36?C for 30 minutes, but you need to control the temp. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sayeed, Mohammed Sent: Tuesday, April 05, 2005 10:56 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] need a good protocol for apoptosis I need a protocol for apoptosis for paraffin embedded tissue. Has incubating paraffin sections at 50 degrees for a certain length of time any effect on the out come of the apoptosis staining? Thanks. M. Sayeed Histology lab Manager Gustavo Ayala, M.D. Associate Professor Department of Pathology and Scott Department Of Urology Baylor College of Medicine One Baylor Plaza Houston, TX 77030 713-798-3705 (W) 713-318-5668 (PGR) 713-444-9887 (Cel) Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains either private, confidential or privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. From GDawson <@t> dynacaremilwaukee.com Wed Apr 6 07:54:03 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] automated versus manual for practical(practicaltes tingcenters?) Message-ID: Connie, What did you do? I would suggest terminating him/her immediately. You couldn't even demote that person to a lab aide in my opinion since not knowing how to de-paraffinize slides by hand would disqualify them for that job as well. That level of ignorance is a slap in the face to all histotechs and screams of problems with this individual down the line so nip it in the bud. To not even know how to de-paraffinize shows a complete disregard for knowing the very basics of histology and a lack of ability to put 2 and 2 together. Any registered histotech that does not know how to de-paraffinize without the aid of a machine should have their certification revoked and needs to pursue another career choice. Just My Opinion, Glen Dawson -----Original Message----- From: connie grubaugh [mailto:conniegrubaugh@hotmail.com] Sent: Tuesday, April 05, 2005 4:54 PM To: JWEEMS@sjha.org; MadaryJ@MedImmune.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] automated versus manual for practical(practicaltestingcenters?) I think it is past time to stop the practical. So many of the stains are done on machines. I have had a " registered tech," that did not know how to run slides manually to just de-parffinize them. Why can't there just be more questions on the exam of theory ect. ect. ect. And that is my 2 cents worth....... Connie G. >From: "Weems, Joyce" >To: "Madary, Joseph" , >Subject: RE: [Histonet] automated versus manual for practical(practicaltestingcenters?) >Date: Tue, 5 Apr 2005 15:42:36 -0400 > >Perhaps its time to stop the practical - do the other disciplines do a practical? It's all so subjective. I think our problem as a laboratory science is that we do not make diagnoses - that sets us apart from the others. And some pathologists have promoted the separation by not respecting us for how important we are to them. My belief is that a pathologist is only as good as the tech. Another 2 cents worth. > >Joyce > >Joyce Weems >Pathology Manager >Saint Joseph's Hospital >5665 Peachtree Dunwoody Rd NE >Atlanta, GA 30342 >404-851-7376 - Phone >404-851-7831 - Fax > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Madary, >Joseph >Sent: Tuesday, April 05, 2005 3:12 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] automated versus manual for practical(practical >testingcenters?) > > >As most I did all three practicals manually getting the first in 1982. I have to say though as a confirmed cheapskate what of the folks that rely on the lab they are in to do the work and the supervisor will not approve of purchasing reagents for manual, and even if the person paid, would have an issue with the the regs/safety issues of getting new chemicals in the lab? I am all for punishing new people in the lab and making their life difficult espceially those non certified types, oh my God! What if they have no access to manual methods? Maybe the folks using automated methods should have to do more or something. How far does it go, coverslipping? I mean one could argue buying a staining kit is just as bad as automation since you are not` making reagents. I think this is an important issue as you all have pointed out. Personally, I feel there should be testing centers for the practical. Hey there is a money making idea that someone can steal from me! > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. >Thank you. Saint Josephs Health System, Inc. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andromeda_tm <@t> libero.it Wed Apr 6 08:04:11 2005 From: andromeda_tm <@t> libero.it (Massimo) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Staining... Message-ID: Torino 06 April 2005 (ITALY) Greetings to everyone, I am looking at an optical microscope a prepared slice with a thin section of a rat kidney. I do not know the origin of the specimen. Its colour in transmitted light is between cyan and green. Does someone tell me what kind of staining has been used? Any suggestions would be appreciated. Thank you. Best Regards, Massimo -------------------------- "Il faut garder sa libert? d'esprit et croire que dans la nature l'absurde suivant nos th?ories n'est pas toujours impossible." (Claude Bernard) ____________________________________________________________ 6X velocizzare la tua navigazione a 56k? 6X Web Accelerator di Libero! Scaricalo su INTERNET GRATIS 6X http://www.libero.it From Michele_Marggi <@t> ssmhc.com Wed Apr 6 08:37:17 2005 From: Michele_Marggi <@t> ssmhc.com (Michele_Marggi@ssmhc.com) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] who reads what? Message-ID: Hello all: I am hoping that I can get some help..... I am wondering what system people are using to divide up cases/slides to your pathologists. We currently have a very "painful" process of dividing up cases to our pathologists. Trying to make it fair in terms of numbers, types of cases, and even trying to accommodate personal interests or specialties. All of this consideration means a lot of time added to the process. I would really appreciate some response and ideas for a better and more efficient process. Thanks, Michele Marggi Surgical Pathology Supervisor St. Marys Hospital Medical Center 707 S Mills Street Madison WI 53715 Telephone: 608.258.6930 Fax: 608.258.6268 From andromeda_tm <@t> libero.it Wed Apr 6 08:40:56 2005 From: andromeda_tm <@t> libero.it (Massimo) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Staining... Message-ID: Torino 06 April 2005 (ITALY) Greetings to everyone, I am looking at an optical microscope a prepared slice with a thin section of a rat kidney. I do not know the origin of the specimen. Its colour in transmitted light is between cyan and green. Does someone tell me what kind of staining has been used? Any suggestions would be appreciated. Thank you. Best Regards, Massimo -------------------------- "Il faut garder sa libert? d'esprit et croire que dans la nature l'absurde suivant nos th?ories n'est pas toujours impossible." (Claude Bernard) ____________________________________________________________ 6X velocizzare la tua navigazione a 56k? 6X Web Accelerator di Libero! Scaricalo su INTERNET GRATIS 6X http://www.libero.it From kelly.mcqueeney <@t> bms.com Wed Apr 6 08:46:11 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Cryosectioning cryoprotected (30% sucrose) brain In-Reply-To: <6.0.0.22.1.20050405154617.01b18858@gemini.msu.montana.edu> References: <200504052029.j35KTXGS013331@pop19.ucdavis.edu> <6.0.0.22.1.20050405154617.01b18858@gemini.msu.montana.edu> Message-ID: <4253E823.6090706@bms.com> I also had some problems sectioning rat brain in the cryostat after perfusion. I got a lot of folding and curling (always 20 micron). This may be due to the temperature in the cryostat. I sectioned the fixed tissue at the same temperature we section fresh tissue. We section fresh tissue at knife = -13, specimen = -8 with one cryostat and knife = -15, specimen = -11 with our other cryostat. The optimal cutting temperature always varies from one cryostat to another, it is important to play around with the temperature if you are getting folding or cracking.. Gayle, do you use -17C for specimen and knife (chamber) temperature? Thanks, Kelly Gayle Callis wrote: > Ethylene glycol is anti-freeze - don't use it. Just let brain > cryoprotect in 30% sucrose - we never bother with a sucrose gradient > anymore but you can use it if you want. > > You should snap freeze the brain properly and then get good frozen > sections at that thickness at approx -17C. but if you have > anti-freeze in tissue, you are probably cutting mush at that temperature. > > > At 02:29 PM 4/5/2005, you wrote: > >> Hello All, >> >> >> >> I am attempting to slice sheep brainstem and hypothalamus on a >> cryostat at >> -20 C. The tissue was treated as such: >> >> >> >> Perfusion through carotid artery (6L of 4% paraformaldehyde) >> >> Postfixation for 24 hours at 4 C >> >> Cryoprotected in a 20% sucrose solution for 5 days at 4 C >> >> Cryoprotected in a 30% sucrose 30% ethylene glycol solution and >> stored at >> -20 C. >> >> >> >> I am having some major problems with slicing this tissue. It appears to >> never freeze. Does anyone know at what temperature I should be slicing? >> Any opinions on use of a vibratome instead of a cryostat? I'm trying to >> section tissue at 30-40 um. Thanks in advance for your help. >> >> >> >> Ben Renquist >> >> UCDavis >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Traczyk7 <@t> aol.com Wed Apr 6 09:00:45 2005 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Looking for Laurie Starkus Message-ID: <141.432d94da.2f85458d@aol.com> Laurie if you are out there, or someone knows where she is, please contact me. Thanks, Dorothy Dorothy Murphy Traczyk Hacker Instruments & Industries Inc. From gu.lang <@t> gmx.at Wed Apr 6 09:19:56 2005 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 16 15:24:55 2005 Subject: AW: [Histonet] who reads what? In-Reply-To: Message-ID: Hi We had the same system. But now we are lucky to deliver all cases to one pathologist. The others go to her room and take their part. The person, who reads the slides is in most times not the same, that did the grossing. Gudrun Lang Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Michele_Marggi@ssmhc.com Gesendet: Mittwoch, 06. April 2005 15:37 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] who reads what? Hello all: I am hoping that I can get some help..... I am wondering what system people are using to divide up cases/slides to your pathologists. We currently have a very "painful" process of dividing up cases to our pathologists. Trying to make it fair in terms of numbers, types of cases, and even trying to accommodate personal interests or specialties. All of this consideration means a lot of time added to the process. I would really appreciate some response and ideas for a better and more efficient process. Thanks, Michele Marggi Surgical Pathology Supervisor St. Marys Hospital Medical Center 707 S Mills Street Madison WI 53715 Telephone: 608.258.6930 Fax: 608.258.6268 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Wed Apr 6 09:47:18 2005 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] RE: Staining... Message-ID: <235ee0235870.235870235ee0@amc.uva.nl> Hi Massimo, You're sure your colleagues didn't make a late April 1st joke with you? In terms of immunohistochemistry the cyan - green color can be either peroxidase activity with tetramethylbenzidine as chromogen or beta-galatosidase activity with X-gal+ferri-ferro cyanide as chromogen. Chris van der Loos, PhD Dept. of Pathology Academical Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 fax: +31 20 6960389 ----- Original Message ----- From "Massimo" Date Wed, 6 Apr 2005 15:40:56 +0200 To "ML Histonet" Subject [Histonet] Staining... Torino 06 April 2005 (ITALY) Greetings to everyone, I am looking at an optical microscope a prepared slice with a thin section of a rat kidney. I do not know the origin of the specimen. Its colour in transmitted light is between cyan and green. Does someone tell me what kind of staining has been used? Any suggestions would be appreciated. Thank you. Best Regards, Massimo -------------- next part -------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tompset2 <@t> msu.edu Wed Apr 6 10:07:34 2005 From: tompset2 <@t> msu.edu (Amber R Tompsett) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Cryosectioning Whole Fish Message-ID: Hello Histonetters, I am currently trying to develop a method to cryosection whole Japanese medaka (a guppie-sized fish). We would eventually like to use labeled probes to do in-situ hybridization on the sections. We have tried sectioning with a disposable steel blade at multiple temperatures and section thicknesses. The muscle seems to section fine, but the organs in the body cavity and the brain (which are the parts of the fish we are interested in) shred or just completely disappear. Any ideas about what is happening or suggestions on how to get better sections? Thanks, Amber Tompsett Graduate Assistant Department of Zoology Michigan State University From gcallis <@t> montana.edu Wed Apr 6 10:07:32 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Re: zincTris fixation on frozen sections In-Reply-To: <6a48b3695164cba5657f40248038766e@umn.edu> References: <6a48b3695164cba5657f40248038766e@umn.edu> Message-ID: <6.0.0.22.1.20050406083918.01b68058@gemini.msu.montana.edu> Yves, We have tried what you want to do, without success. For answer to question #1 - IF you try this, NO you would just rinse in TRIS buffered saline X 3 after ZnTRIS fixation. You should do a time panel to determine if this is going to work, 2,4,6,8,10, 15, 30 minutes - whatever. I have never seen this fixative used for fresh frozen sections, only tissues destined for paraffin embedding. Further comments next. For answer to question #2, when we tried ZnTRIS on fresh frozen sections murine tissue for CD markers, it was a total failure. Morphology was no better and IHC staining was poor and diffuse. We only do solvent fixation for surface markers. If we used ZnTRIS , it would only be for paraffin work to avoid NBF for CD markers. Although touted to have good morphology with paraffin sections, we have not found that to be true, plus the mouse tissues were dry, friable and difficult to cut. We did get staining of CD markers though. We found it much faster with better results to do use the following suggestions. You can try other fixation protocols to try and improve the morphology 1. An acetone/alcohol fixation (AA) Air dry FS overnight at RT, they MUST BE VERY DRY. Fix next day (of staining) in 75% acetone/25% absolute ethanol or 75 ml acetone mixed with 25 ml 100% ethanol (do NOT use methanol or any other alcohol) . Fix for 5 minutes, then go directly to buffer rinse. DO NOT air dry sections again, and proceed to staining. 2. A double acetone fixation protocol works also, although morphology is not as good as AA fixation. We rarely do single acetone fixation, and if we do it, very dry FS in 4C acetone for 10 min, air dry and stain. The double acetone protocol is: Air dry FS overnight at RT (over 16 mesh silica gel), then fix in 4C acetone for 10 min, air dry 15 min in front of fan if possible, go back to acetone for 10 min at 4C, air dry 20 min and proceed with staining. It is a dilemma, but we live with some loss of morphology and excellent IHC staining for all our cell surface markers, an unfortunate circumstance when working with certain surface markers in the mouse. Overall, our sections with AA are excellent, although some surface markers may not like the alcohol component, and others may like only minimal fixation in cold acetone - a lot depends on the antigen. We have even found CD markers that like AA better than vendor suggested cold acetone fixation, although they say it will not work. Rule is TRY IT!!! At 09:22 PM 4/5/2005, you wrote: >I am in the process of staining mouse kidney samples containing grafted >mouse pancreatic islets with antibodies against MHC H2Dd and H2Db. Tissue >samples available are either FFPE or unfixed, snap frozen. I decided to >concentrate on the frozen samples due to the general difficulty of >staining for surface markers after formalin fixation. My questions are: > >1. If I want to use frozen sections, immediately fixed in zincTris >(formalin-free), will I need to process the slides through increasing >alcohols just as suggested for zincTris fixation of tissue samples ? >2. Will zincTris fixation yield better morphology compared to conventional >acetone fixation ? > >Yves Heremans >University of Minnesota >Stem Cell Institute >Tel 612-625-0964 >Fax 612-624-2436 Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From abright <@t> brightinstruments.com Wed Apr 6 10:21:12 2005 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Cryosectioning Whole Fish Message-ID: Dear Amber, What is the temperature of the specimen and the cryochamber? Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com -----Original Message----- From: Amber R Tompsett [mailto:tompset2@msu.edu] Sent: 06 April 2005 16:08 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryosectioning Whole Fish Hello Histonetters, I am currently trying to develop a method to cryosection whole Japanese medaka (a guppie-sized fish). We would eventually like to use labeled probes to do in-situ hybridization on the sections. We have tried sectioning with a disposable steel blade at multiple temperatures and section thicknesses. The muscle seems to section fine, but the organs in the body cavity and the brain (which are the parts of the fish we are interested in) shred or just completely disappear. Any ideas about what is happening or suggestions on how to get better sections? Thanks, Amber Tompsett Graduate Assistant Department of Zoology Michigan State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Wed Apr 6 10:24:51 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] who reads what? Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB421B@fh2k093.fhmis.net> We used to distribute by 20-slide trays - taking into consideration the Histology slides only. Then, because the Pathologists also had Cytology, Bone Marrows, FNAs, Flow Cytometry and Peripheral smears to read, we now distribute the night before by slide count, using Pathology/Cytology dictation, the Copath System, and verbal call-ins - taking into consideration ALL the slides the Pathologists have to read. I believe we're close to 1500 slides per day to distribute among 14 Pathologists. -Janet -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: 4/6/2005 9:37 AM Subject: [Histonet] who reads what? Hello all: I am hoping that I can get some help..... I am wondering what system people are using to divide up cases/slides to your pathologists. We currently have a very "painful" process of dividing up cases to our pathologists. Trying to make it fair in terms of numbers, types of cases, and even trying to accommodate personal interests or specialties. All of this consideration means a lot of time added to the process. I would really appreciate some response and ideas for a better and more efficient process. Thanks, Michele Marggi Surgical Pathology Supervisor St. Marys Hospital Medical Center 707 S Mills Street Madison WI 53715 Telephone: 608.258.6930 Fax: 608.258.6268 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Apr 6 10:34:39 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Super frost plus charge slides from Erie versus others Message-ID: <6.0.0.22.1.20050406091859.01b681b0@gemini.msu.montana.edu> If the name on the slide box is Super Frost Plus Charge, these are made by Erie, under registered trademark. The key is to look for that trademark. If one has doubts about who makes the slides, ask a vendor or call Erie. Under VWR and Fisherbrand labels, with box also saying Super Frost Plus Charge all have the registered trademark = Erie manufactured slides. VWR and Fisher are not the only ones who buy slides from Erie and have the company name (not Erie) on slide boxes. Sometimes these slides are custom designs for particular immunostainers. One comes to mind here. Key to identity: look for that trademark, even though the company name is different. If the name if NOT Super Frost Plus Charge, then it is NOT an Erie product. A very confusing issue at times. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From jdmd77 <@t> hotmail.com Wed Apr 6 10:38:57 2005 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] who reads what? In-Reply-To: Message-ID: Hello Michele - Though it may be impossible in your situation - please remember that it is the responsibility of the pathologists to determine and agree upon their group and individual workload and schedules. Though frequently this is solely based upon seniority or politics within the group - the ideal circumstance is when the workload is based upon the best interest of patient care. When pathologists have "areas of interest" it means that they are generally better at diagnosing diseases of those particular body organs. Those interests translate to better patient care, since the best possible pathologist in the group is looking at the type of pathology they have the greatest knowledge base in; rather than just being a symptom of a finnicky pathologist. The director of pathology should be involved in establishing the "routine" for delegating slide distribution. Is this the case in your institution? Julia Dahl MD >From: Michele_Marggi@ssmhc.com >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] who reads what? >Date: Wed, 6 Apr 2005 08:37:17 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by >mc10-f38.hotmail.com with Microsoft SMTPSVC(6.0.3790.211); Wed, 6 Apr 2005 >06:38:55 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1DJAjf-00008m-N4; Wed, 06 Apr >2005 08:38:28 -0500 >Received: from [199.165.152.167] (helo=swlx167.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1DJAjC-0008Vb-OPfor >histonet@lists.utsouthwestern.edu; Wed, 06 Apr 2005 08:38:09 -0500 >Received: from [67.66.142.138] (helo=ssmhc.com)by swlx167.swmed.edu with >esmtp (Exim 4.44) id 1DJAjB-0006x2-Dbfor histonet@lists.utsouthwestern.edu; >Wed, 06 Apr 2005 08:37:54 -0500 >Received: from ([165.104.141.6])by mail01.ssmhc.com with ESMTP id >KP-KZ182.27898934;Wed, 06 Apr 2005 08:37:16 -0500 >Received: from smhmcntn1.smhmc.ssmhc.com ([172.28.5.6])by stlhub.ssmhc.com >(Lotus Domino Release 6.5.1)with ESMTP id 2005040608371603-26547 ;Wed, 6 >Apr 2005 08:37:16 -0500 X-Message-Info: >skrQa+6szm3dLltnGS8PVCLgjqMNIRXmysCN8IvzDag= >X-Mailer: Lotus Notes Release 5.0.4 June 8, 2000 >X-MIMETrack: Serialize by Router on SMHMCNTN1/SSMHC(Release >6.5.3FP1|December15, 2004) at 04/06/2005 08:37:19 AM,Itemize by SMTP Server >on stlhub/SSMHC(Release 6.5.1|January 21,2004) at 04/06/2005 08:37:16 >AM,Serialize by Router on stlhub/SSMHC(Release 6.5.1|January 21,2004) at >04/06/2005 08:37:16 AM,Serialize complete at 04/06/2005 08:37:16 AM >X-Scan-Signature: 02458d915c013d2bafb5a1ca86d08841 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: fafe997e7c8aede70e4d53d18f8e9e53 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.3 required=5.5 tests=NO_REAL_NAME >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 06 Apr 2005 13:38:56.0159 (UTC) >FILETIME=[FACF9AF0:01C53AAD] > >Hello all: > >I am hoping that I can get some help..... > >I am wondering what system people are using to divide up cases/slides to >your pathologists. We currently have a very "painful" process of dividing >up cases to our pathologists. Trying to make it fair in terms of numbers, >types of cases, and even trying to accommodate personal interests or >specialties. All of this consideration means a lot of time added to the >process. I would really appreciate some response and ideas for a better >and more efficient process. > >Thanks, > >Michele Marggi >Surgical Pathology Supervisor >St. Marys Hospital Medical Center >707 S Mills Street >Madison WI 53715 >Telephone: 608.258.6930 >Fax: 608.258.6268 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Wed Apr 6 10:38:35 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] who reads what? Message-ID: That's not a histology lab. - that's a factory. Good God - how do you cope? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bonner, Janet [mailto:Janet.Bonner@FLHOSP.ORG] Sent: 06 April 2005 16:25 To: 'Michele_Marggi@ssmhc.com '; 'histonet-bounces@lists.utsouthwestern.edu '; 'histonet@lists.utsouthwestern.edu ' Subject: RE: [Histonet] who reads what? We used to distribute by 20-slide trays - taking into consideration the Histology slides only. Then, because the Pathologists also had Cytology, Bone Marrows, FNAs, Flow Cytometry and Peripheral smears to read, we now distribute the night before by slide count, using Pathology/Cytology dictation, the Copath System, and verbal call-ins - taking into consideration ALL the slides the Pathologists have to read. I believe we're close to 1500 slides per day to distribute among 14 Pathologists. -Janet -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: 4/6/2005 9:37 AM Subject: [Histonet] who reads what? Hello all: I am hoping that I can get some help..... I am wondering what system people are using to divide up cases/slides to your pathologists. We currently have a very "painful" process of dividing up cases to our pathologists. Trying to make it fair in terms of numbers, types of cases, and even trying to accommodate personal interests or specialties. All of this consideration means a lot of time added to the process. I would really appreciate some response and ideas for a better and more efficient process. Thanks, Michele Marggi Surgical Pathology Supervisor St. Marys Hospital Medical Center 707 S Mills Street Madison WI 53715 Telephone: 608.258.6930 Fax: 608.258.6268 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michele_Marggi <@t> ssmhc.com Wed Apr 6 10:46:50 2005 From: Michele_Marggi <@t> ssmhc.com (Michele_Marggi@ssmhc.com) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] who reads what? Message-ID: Yes- our director is involved. I just knew the histonet would be a great starting point- and I was right....I appreciate all the responses, and look forward to more. Thanks! Michele Marggi Surgical Pathology Supervisor St. Marys Hospital Medical Center 707 S Mills Street Madison WI 53715 Telephone: 608.258.6930 Fax: 608.258.6268 "Julia Dahl" cc: Subject: RE: [Histonet] who reads what? 04/06/2005 10:38 AM Hello Michele - Though it may be impossible in your situation - please remember that it is the responsibility of the pathologists to determine and agree upon their group and individual workload and schedules. Though frequently this is solely based upon seniority or politics within the group - the ideal circumstance is when the workload is based upon the best interest of patient care. When pathologists have "areas of interest" it means that they are generally better at diagnosing diseases of those particular body organs. Those interests translate to better patient care, since the best possible pathologist in the group is looking at the type of pathology they have the greatest knowledge base in; rather than just being a symptom of a finnicky pathologist. The director of pathology should be involved in establishing the "routine" for delegating slide distribution. Is this the case in your institution? Julia Dahl MD >From: Michele_Marggi@ssmhc.com >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] who reads what? >Date: Wed, 6 Apr 2005 08:37:17 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by >mc10-f38.hotmail.com with Microsoft SMTPSVC(6.0.3790.211); Wed, 6 Apr 2005 >06:38:55 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1DJAjf-00008m-N4; Wed, 06 Apr >2005 08:38:28 -0500 >Received: from [199.165.152.167] (helo=swlx167.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1DJAjC-0008Vb-OPfor >histonet@lists.utsouthwestern.edu; Wed, 06 Apr 2005 08:38:09 -0500 >Received: from [67.66.142.138] (helo=ssmhc.com)by swlx167.swmed.edu with >esmtp (Exim 4.44) id 1DJAjB-0006x2-Dbfor histonet@lists.utsouthwestern.edu; >Wed, 06 Apr 2005 08:37:54 -0500 >Received: from ([165.104.141.6])by mail01.ssmhc.com with ESMTP id >KP-KZ182.27898934;Wed, 06 Apr 2005 08:37:16 -0500 >Received: from smhmcntn1.smhmc.ssmhc.com ([172.28.5.6])by stlhub.ssmhc.com >(Lotus Domino Release 6.5.1)with ESMTP id 2005040608371603-26547 ;Wed, 6 >Apr 2005 08:37:16 -0500 X-Message-Info: >skrQa+6szm3dLltnGS8PVCLgjqMNIRXmysCN8IvzDag= >X-Mailer: Lotus Notes Release 5.0.4 June 8, 2000 >X-MIMETrack: Serialize by Router on SMHMCNTN1/SSMHC(Release >6.5.3FP1|December15, 2004) at 04/06/2005 08:37:19 AM,Itemize by SMTP Server >on stlhub/SSMHC(Release 6.5.1|January 21,2004) at 04/06/2005 08:37:16 >AM,Serialize by Router on stlhub/SSMHC(Release 6.5.1|January 21,2004) at >04/06/2005 08:37:16 AM,Serialize complete at 04/06/2005 08:37:16 AM >X-Scan-Signature: 02458d915c013d2bafb5a1ca86d08841 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >,< mailto:histonet-request@lists.utsouthwestern.edu?subject=subscribe> >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: fafe997e7c8aede70e4d53d18f8e9e53 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.3 required=5.5 tests=NO_REAL_NAME >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 06 Apr 2005 13:38:56.0159 (UTC) >FILETIME=[FACF9AF0:01C53AAD] > >Hello all: > >I am hoping that I can get some help..... > >I am wondering what system people are using to divide up cases/slides to >your pathologists. We currently have a very "painful" process of dividing >up cases to our pathologists. Trying to make it fair in terms of numbers, >types of cases, and even trying to accommodate personal interests or >specialties. All of this consideration means a lot of time added to the >process. I would really appreciate some response and ideas for a better >and more efficient process. > >Thanks, > >Michele Marggi >Surgical Pathology Supervisor >St. Marys Hospital Medical Center >707 S Mills Street >Madison WI 53715 >Telephone: 608.258.6930 >Fax: 608.258.6268 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dobbin <@t> upei.ca Wed Apr 6 12:18:54 2005 From: dobbin <@t> upei.ca (Greg Dobbin) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Cryosectioning Whole Fish Message-ID: <4253E1BE.9147.F61D48@localhost> ------- Forwarded message follows ------- From: Greg Dobbin To: "Amber R Tompsett" Subject: Re: [Histonet] Cryosectioning Whole Fish Date sent: Wed, 6 Apr 2005 13:18:12 -0400 Hi Amber, Would it be too disruptive to your study to inject a small amount of cryomatrix intra-abdominally prior to freezing? I think the internal organs, being small and fragile, are not sticking to one another when frozen. I also suspect there is quite a high fat component in there as well, so with fat, a colder cutting temperature is generally better. By the way, I haven't tried the intra-abdominal injection technique myself. Let me know if you try it and how well it works. Good luck. Greg From: "Amber R Tompsett" To: histonet@lists.utsouthwestern.edu Date sent: Wed, 06 Apr 2005 11:07:34 -0400 Subject: [Histonet] Cryosectioning Whole Fish > Hello Histonetters, > > I am currently trying to develop a method to cryosection whole > Japanese medaka (a guppie-sized fish). We would eventually like to > use labeled probes to do in-situ hybridization on the sections. We > have tried sectioning with a disposable steel blade at multiple > temperatures and section thicknesses. The muscle seems to section > fine, but the organs in the body cavity and the brain (which are the > parts of the fish we are interested in) shred or just completely > disappear. > > Any ideas about what is happening or suggestions on how to get > better sections? > > Thanks, > Amber Tompsett > Graduate Assistant > Department of Zoology > Michigan State University > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------- End of forwarded message ------- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Greg Dobbin Research Technologist, Pathology Lab, Atlantic Veterinary College, U.P.E.I. 550 University Ave. Charlottetown, P.E.I. Canada, C1A 4P3 Phone: (902)566-0744 Fax: (902)566-0851 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Happiness is a journey, not a destination. From Rcartun <@t> harthosp.org Wed Apr 6 11:34:22 2005 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] antibody Message-ID: I would check with Dr. David Walker at the University of Texas Medical Branch in Galveston. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 04/05/05 11:31AM >>> Does anyone know who performs IHC testing for rickettsieae..we checked with Mayo and Propath..any ideas and thanks!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------- Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From petepath <@t> yahoo.com Wed Apr 6 11:46:35 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] who reads what? Message-ID: <20050406164635.65112.qmail@web30405.mail.mud.yahoo.com> I have a simple spread sheet that lists the pathologist names on the y axis and the cases which are destributed to them on the X axis. The chart numbes cases consecutively. Each pathologist has a pre determined number that are filled into his boxes on the chart when they are accessioned. For instance If i am on our surge path 1 rotation I will reciece #s 1-10, 30 -40, 60 -70, 80 -90, 101-105....... Depending on the total for the day will recieve further #s on this chart. The other 5 pathologists recieving cases will also have designated #s on this chart. As soon as the case os accessioned, the number is filled in on the chart. Any cases which I read exclusively ( such as ped GI) are placed in my boxes even if out of order. So you are left with a big spread sheet filled in with the cases of the day. As soon as they are accessioned my transcribers, PA's residents and clinicians know who to go to with questions. We have different flow charts depending on how many we have this dat. % man, 6 man and 7 man rotations. It sounds complicated but is truely an easy way to get this complicated job done. I have a copy of the chart that I underline when a case is brought to me so I always know if it is really my case they are bringing me before I read it. Before this I would find I read two trays of someone elses work and wonder why I seem to have so much that day. I know this sounds complicated but I can send an example of the chart to any one who would like. I am sending one to Michelle. Maybe she will give us some feed back to see if it works for her. From jdmd77 <@t> hotmail.com Wed Apr 6 12:12:46 2005 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] who reads what? In-Reply-To: Message-ID: Unfortunately - a great deal of the pathology environment IS becoming a "factory." Hospitals own the histology laboratories in many situations - requiring incredible feats to overcome bureaucratic resistance to adequate staffing - and on the outpatient side, many pathologists in the US are client billing (allowing the clinician to bill for pathology services) or forming "pod laboratories" where the clinician is "owner" of the histology laboratory - essentially providing kick-backs to the clinicians for sending the biopsy material to that outpatient pathology laboratory... This translates to PATHOLOGIST DRIVEN decimation of reimbursement (tell me, folks - in whose mind could it possibly be SANE to negotiate for a lower reimbursement than what we struggle to get from the insurance companies - and call it "a businessman's dream"?). Insurance or pathologist driven declines in reimbursement translate to substantial increases in volume daily for each histotechnologist and each pathologist. The factory model has some advantages: Histotechs who are more skilled at handling smaller biopsy specimens can "subspecialize" and cut prostates, breast cores, GIs and such... while other histotechs may be particularly skilled at cutting larger surgicals without wrinkles, chatter or holes. Pathologists in these "factory" settings may also subspecialize - but I'm not seeing as much of that as you'd expect. The subspecialty becomes "general biopsy pathologist" - splitting a days work between prostate needle cores, breast biopsies, GI biopsies and skin. The jack of all trades is never master of ONE... I think that this translates to risk to patient care. As a practicing pathologist - I don't balk at reviewing 100+ slides/day - as long as they are in my area of "expertise" (GI/liver). But hand me 100 pap smears and that will take me 10 hours.... 100 skins and maybe I could sign that out in a week. Though there was a push to primary care and "generalist" medicine while I was in medical school in the 90s - I'm a firm believer and practicer of subspecialization for the sake of better patient care and, believe it or not - EFFICIENCY. Until more pathologists are willing to say "No way" to client billing, "Not a chance" to pod laboratories and to be involved locally in resisting HMO capitated contracts that give exclusive contracts to Quest/LabCorp, etc. for 100% of clinical and anatomic services - the factory model will increase and GET significantly worse. The minimum number of CASES (not slides - remember that some cases require 3 slides - 2 levels and 1 special stain) - for a factory pathologist is 100. That's the minimum. And the compensation for those 100 cases: Certainly not the $10,000. of income that that generates (average reimbursement for a 88305 [skin, GI, breast core] is $100). Between the client billing arrangements that many of the factories agree to ($50 for the pathology group - $50 for the gastroenterologist who sends the biopsy), and the capitated contracts with HMOs, the income available to histotechs, PAs, grossing clerks, administrative support staff, couriers and pathologists is gouged by the CEO of factory laboratory - marketing, lawyers to defend against OIG investigations and lobbyists to paint a rosy picture to the government. Someone in each laboratory (and hopefully it's a top down phenomenon) has to take interest in what is best for the patient - in each case - every day. Isn't that what made medicine so appealing as a career in the first place? JD >From: "Marshall Terry Dr,Consultant Histopathologist" > >To: "Bonner, Janet" , >,, >Subject: RE: [Histonet] who reads what? >Date: Wed, 6 Apr 2005 16:38:35 +0100 >MIME-Version: 1.0 > >That's not a histology lab. - that's a factory. >Good God - how do you cope? > >Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > >-----Original Message----- >From: Bonner, Janet [mailto:Janet.Bonner@FLHOSP.ORG] >Sent: 06 April 2005 16:25 >To: 'Michele_Marggi@ssmhc.com '; >'histonet-bounces@lists.utsouthwestern.edu '; >'histonet@lists.utsouthwestern.edu ' >Subject: RE: [Histonet] who reads what? > > > We used to distribute by 20-slide trays - taking into consideration the >Histology slides only. Then, because the Pathologists also had Cytology, >Bone Marrows, FNAs, Flow Cytometry and Peripheral smears to read, we now >distribute the night before by slide count, using Pathology/Cytology >dictation, the Copath System, and verbal call-ins - taking into >consideration ALL the slides the Pathologists have to read. I believe we're >close to 1500 slides per day to distribute among 14 Pathologists. > > -Janet > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >To: histonet@lists.utsouthwestern.edu >Sent: 4/6/2005 9:37 AM >Subject: [Histonet] who reads what? > >Hello all: > >I am hoping that I can get some help..... > >I am wondering what system people are using to divide up cases/slides >to >your pathologists. We currently have a very "painful" process of >dividing >up cases to our pathologists. Trying to make it fair in terms of >numbers, >types of cases, and even trying to accommodate personal interests or >specialties. All of this consideration means a lot of time added to the >process. I would really appreciate some response and ideas for a >better >and more efficient process. > >Thanks, > >Michele Marggi >Surgical Pathology Supervisor >St. Marys Hospital Medical Center >707 S Mills Street >Madison WI 53715 >Telephone: 608.258.6930 >Fax: 608.258.6268 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Wed Apr 6 12:11:03 2005 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Clinical Lab Message-ID: <002901c53acb$9dcb84e0$7c01a8c0@biopath.org> Does anyone know if there is a server like this one where people post questions to a group of subscribers in the clinical side of the lab (i.e. chemistry, blood, etc.). I know of someone who is looking at purchasing equipment for the clinical lab and she wants to get feedback from others who have experience with the various types that are out there. Thanks, Paula Bio-Path Medical Group Fountain Valley, CA From jdmd77 <@t> hotmail.com Wed Apr 6 12:17:16 2005 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] who reads what? In-Reply-To: <20050406164635.65112.qmail@web30405.mail.mud.yahoo.com> Message-ID: Your methodology looks really functional - particularly that it allows the HTs and Pathologists to track what case is where at any time... instead of "Who's looking at the case on Mr. Jones" being blared over the department loudspeakers. How did you become interested in pediatric GI? Julia >From: "Stephen Peters M.D." >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] who reads what? >Date: Wed, 6 Apr 2005 09:46:35 -0700 (PDT) >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by mc2-f22.hotmail.com >with Microsoft SMTPSVC(6.0.3790.211); Wed, 6 Apr 2005 10:13:10 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1DJDgT-0007ej-45; Wed, 06 Apr >2005 11:47:26 -0500 >Received: from [199.165.152.166] (helo=swlx166.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1DJDft-0007ec-J5for >histonet@lists.utsouthwestern.edu; Wed, 06 Apr 2005 11:46:56 -0500 >Received: from [68.142.200.108] (helo=web30405.mail.mud.yahoo.com)by >swlx166.swmed.edu with smtp (Exim 4.44) id 1DJDfn-0005Ju-VRfor >histonet@lists.utsouthwestern.edu; Wed, 06 Apr 2005 11:46:41 -0500 >Received: (qmail 65114 invoked by uid 60001); 6 Apr 2005 16:46:35 -0000 >Received: from [69.74.36.202] by web30405.mail.mud.yahoo.com via HTTP;Wed, >06 Apr 2005 09:46:35 PDT >X-Message-Info: skrQa+6szm2W48mVszJgdmSSU6lG/CSJSvolL+cJspo= >Comment: DomainKeys? See http://antispam.yahoo.com/domainkeys >DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=s1024; >d=yahoo.com;b=wyKRnOS0pCRfa5IoNrLrFdj/y2CjjzBGYczy2GJpMKK8NEd0OkxCLspriKdr0fJk5gpAlEJlVRT4IbCK+aoaU5uoZZatBqPdboFaCBdUNTmalBeq6I7rjhQdyayHk7J8U44Q8mll2rJ4zOiyl4ouhH+Dit0/P6JV5WDYgn9dP50=; >X-Scan-Signature: 8a6fea9a433dc978cbd63de341601038 >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 02458d915c013d2bafb5a1ca86d08841 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.5 required=5.5 >tests=FORGED_YAHOO_RCVD autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 06 Apr 2005 17:13:10.0790 (UTC) >FILETIME=[E8C4A260:01C53ACB] > >I have a simple spread sheet that lists the pathologist names on the y axis >and the >cases which are destributed to them on the X axis. The chart numbes cases >consecutively. Each pathologist has a pre determined number that are filled >into his boxes > on the chart when they are accessioned. For instance If i am on our surge >path 1 rotation I >will reciece #s 1-10, 30 -40, 60 -70, 80 -90, 101-105....... Depending on >the total for the >day will recieve further #s on this chart. The other 5 pathologists >recieving cases will also >have designated #s on this chart. As soon as the case os accessioned, the >number is filled > in on the chart. Any cases which I read exclusively ( such as ped GI) >are placed in my >boxes even if out of order. So you are left with a big spread sheet filled >in with the cases of >the day. As soon as they are accessioned my transcribers, PA's residents >and >clinicians know who to go to with questions. We have different flow charts >depending on >how many we have this dat. % man, 6 man and 7 man rotations. It sounds >complicated but >is truely an easy way to get this complicated job done. I have a copy of >the chart that >I underline when a case is brought to me so I always know if it is really >my case they >are bringing me before I read it. Before this I would find I read two trays >of someone elses >work and wonder why I seem to have so much that day. I know this sounds >complicated but >I can send an example of the chart to any one who would like. >I am sending one to Michelle. Maybe she will give us some feed back to see >if it works for her. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Wed Apr 6 12:19:15 2005 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] who reads what? Message-ID: <1DB04B57B04C5747B87C3B39F3E605AA02E450F9@harrier> Dear Michelle, We had a similar situation here about 5 or 6 years ago. The adjective "painful" is appropriate indeed in describing this problem. I don't know how many pathologists you have on staff but the greater the number the worse the problem is. We had 5 at the time and an additional (conditional) part-time pathologist thrown into the mix. We also had recently hired a PA so he was factored in as well. Obviously, not to read out slides, but for a calculation of grossing bench time for the pathologist "du jour". We ended up with 4 pages of instructions that basically laid out about a dozen various scenarios. These scenarios were divided into thirds with 5 steps of special instructions for each third. Once you determined which one of the 12 (or the closest variation thereof) scenarios existed for that day, you then had a 2 page worksheet to complete with the calculations (number of slides) and to which pathologist they were to go. This became so cumbersome and ridiculous that we did what we had to do...gave it back to the pathologists. Not only was this a valuable waste of our tech time every day, the pathologists for the most part were unhappy. The histotechs conveniently became the "scapegoats" as well in all of this as any unhappiness about the division of labor was squarely placed on their shoulders. After all, they had "divvied" the work up! We have made dramatic increases in production volumes since then and now have 8 pathologists to serve. I can't imagine having to divide the work up for them again. Every service is different and obviously I do not know the philosophy or flow of yours. But with all the variables thrown in (frozen sections, intra-operative consultations, specific case assignments of special interest/specialty, etc., etc.) it is beyond reasonable expectation to have anyone other than the pathologists calculate their division of labor. When we initially met to discuss this with the pathologists a couple were adamantly opposed to doing the division themselves. It soon became apparent that this was not for any practical or process improvement reasons. It was simply because they did not want to do it. Someone had been doing it for them and if they didn't like the way the day shook out it was never their fault. Fortunately, logic and reason ruled the day and we've never looked back (thank the Lord). I'm certain it's apparent that I believe you should put the ball in the pathologists court and have them take this over. I do provide production numbers daily to the pathologists and they take it from there. I don't know what their exact system is but they seem to be doing just fine with it. If your pathologist group is reasonable this may be a solution for you. I hope this is helpful. If you have any questions feel free to contact me. Good luck. Thomas Jasper HT(ASCP)BAS Anatomic Pathology Coordinator SMDC Clinical Laboratory Duluth, MN tjasper@smdc.org -----Original Message----- From: Michele_Marggi@ssmhc.com [mailto:Michele_Marggi@ssmhc.com] Sent: Wednesday, April 06, 2005 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] who reads what? Hello all: I am hoping that I can get some help..... I am wondering what system people are using to divide up cases/slides to your pathologists. We currently have a very "painful" process of dividing up cases to our pathologists. Trying to make it fair in terms of numbers, types of cases, and even trying to accommodate personal interests or specialties. All of this consideration means a lot of time added to the process. I would really appreciate some response and ideas for a better and more efficient process. Thanks, Michele Marggi Surgical Pathology Supervisor St. Marys Hospital Medical Center 707 S Mills Street Madison WI 53715 Telephone: 608.258.6930 Fax: 608.258.6268 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From la.sebree <@t> hosp.wisc.edu Wed Apr 6 12:23:15 2005 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Superfrost Plus slides Message-ID: Other brands work on Ventana instruments also; we've done it. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dave Johnson Sent: Tuesday, April 05, 2005 3:21 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Superfrost Plus slides Anyone know why only the Erie slides work on the Ventana? >From: Julie.Sanders@med.va.gov >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Superfrost Plus slides >Date: Tue, 5 Apr 2005 12:29:02 -0500 > > >We get ours from Fisher, but I believe they're made by Erie Scientific, >you could contact either one for a quote. We get a fairly good price >from Fisher. Julie >Julie Sanders, Supervisor Anatomic Pathology >VAMC, Cincinnati, Ohio > > > >on 4-5-05 you wrote: >We're talking of buying a Ventana Benchmark XT and they insist that we >need to use Superfrost Plus slides. They are going to cost much more >than what we're using now. Does anyone have the inside track on the >least expensive place to buy them from? You folks are always "The" >source for All Histology Wisdom! Thanks! > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Wed Apr 6 12:24:28 2005 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Tech to Path Ratio Message-ID: <1DB04B57B04C5747B87C3B39F3E605AA02E450FA@harrier> Thanks to Denise and Stephen for their responses to my question about Tech to Path Ratio. I've incorporated your information and am doing what I can with what I have for now. Thomas Jasper HT(ASCP)BAS Anatomic Pathology Coordinator SMDC Clinical Laboratory Duluth, MN tjasper@smdc.org This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From Heather.A.Harper <@t> pcola.med.navy.mil Wed Apr 6 12:34:33 2005 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] How to eliminate AFB contamination on slides? Message-ID: <807FE48C5A7CC940B973B58D32E7014318F93F63@nhpens-exch1.pcola.med.navy.mil> We are having problems with the AFB stain. Almost everything we do AFB stains on has extracellular "AFB positive organisms" without an associated tissue response. Do we need to change our stain or use better grade water? Anybody who has any ideas or suggestions or has had this problem in the past, let me know what you did to correct the problem. Thank you to all in advance. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL From Janet.Bonner <@t> FLHOSP.ORG Wed Apr 6 12:50:13 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] How to eliminate AFB contamination on slides? Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4221@fh2k093.fhmis.net> We discovered that using the type of control slide that has the control in a box at the top so we could put the patient section on the bottom of the same slide led to contamination, organisms coming off of the control!! When we stopped doing the above, the contamination stopped. Another suggestion would be to filter your stain before each use. Janet -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: 4/6/2005 1:34 PM Subject: [Histonet] How to eliminate AFB contamination on slides? We are having problems with the AFB stain. Almost everything we do AFB stains on has extracellular "AFB positive organisms" without an associated tissue response. Do we need to change our stain or use better grade water? Anybody who has any ideas or suggestions or has had this problem in the past, let me know what you did to correct the problem. Thank you to all in advance. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Wed Apr 6 12:52:07 2005 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] INKING BREAST SPECIMENS Message-ID: <95111571D1C6B5498C64F68DD0769BB506BEEA@cvm36.vetmed.wsu.edu> Diana, It's been 8 yrs. Since I've been around mastectomy specimens, but we had to use black India ink only because our pathologist told us that India ink was the only dye that didn't give false IHC results... I think due to natural estrogens in some plant derived dyes. Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, April 05, 2005 3:53 PM To: Diana McCaig; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] INKING BREAST SPECIMENS Diana, we just use one color, but we separate the four quadrants and dictate which quadrant is placed in which cassettes. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Diana McCaig" To: Sent: Tuesday, April 05, 2005 2:45 PM Subject: [Histonet] INKING BREAST SPECIMENS > Is there a universal protocol for colors when inking mastectomy > specimens? > Are green, blue, yellow, red and black assigned to a particular aspect of > the specimen or is it pathologists preference? > Thanks > Diana > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Anti-Virus. > Version: 7.0.308 / Virus Database: 266.9.3 - Release Date: 4/5/2005 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Wed Apr 6 12:56:02 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] How to eliminate AFB contamination on slides Message-ID: <20050406175602.10622.qmail@web52606.mail.yahoo.com> Try putting your patient tissue on a seperate slide and filtering the CF before each use. Larry --------------------------------- Yahoo! Messenger Show us what our next emoticon should look like. Join the fun. From petepath <@t> yahoo.com Wed Apr 6 13:29:29 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] who reads what? Message-ID: <20050406182930.46916.qmail@web30415.mail.mud.yahoo.com> I had a number of requests for my accessioning list. It sounds like this is a common problem. If it is OK with histonet users, I will photograph them and put them on a page of my web site with an explanation. This way I only have to type this once. (It is pretty clear how bad I type! ). I will post a link in a day or so when it is ready. Stephen From gcallis <@t> montana.edu Wed Apr 6 13:32:46 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Registered tech without knowledge on deparaffinizing sections In-Reply-To: References: Message-ID: <6.0.0.22.1.20050406093532.01b5afc0@gemini.msu.montana.edu> You are not doing this tech any favors by firing them. Why not take the time to re-teach them, armed with literature to back you up to complete their so called training. This tech is not entirely to blame for not knowing how to deparaffinize sections whether manually or by machine. After all, with registry but lack of know how, this person will merely seek another job and carry on, blissfully unaware and still poorly trained. I would point the fickle finger of fate at where the fault lies in the first place - and it is the laboratory where the tech trained and their "training" methods. I would entertain bets the people responsible for training this person were too busy to explain why/how's of manual/machine section deparaffinization OR they didn't understand deparaffinization either (whew, that would be sad!). Techs in training (or not) should read text books but only if laboratories training people have texts, an outlined study guide, and websites acessible/available - but when it comes to down to performing the actual hands on procedure, the experienced technician(s) involved in training need to stand with them - ready to explain (including any theory!) in detail what is going on. If you hire the person lacking in knowledge, become their mentor, be diplomatic and kind but help/teach people (even registered) the finer, basic points of histotechnics. Our profession will be better for it. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman Bozeman MT 59717-3610 At 06:54 AM 4/6/2005, you wrote: >Connie, > >What did you do? I would suggest terminating him/her immediately. You >couldn't even demote that person to a lab aide in my opinion since not >knowing how to de-paraffinize slides by hand would disqualify them for that >job as well. That level of ignorance is a slap in the face to all >histotechs and screams of problems with this individual down the line so nip >it in the bud. To not even know how to de-paraffinize shows a complete >disregard for knowing the very basics of histology and a lack of ability to >put 2 and 2 together. > >Any registered histotech that does not know how to de-paraffinize without >the aid of a machine should have their certification revoked and needs to >pursue another career choice. > >Just My Opinion, >Glen Dawson > >-----Original Message----- >From: connie grubaugh [mailto:conniegrubaugh@hotmail.com] >Sent: Tuesday, April 05, 2005 4:54 PM >To: JWEEMS@sjha.org; MadaryJ@MedImmune.com; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] automated versus manual for >practical(practicaltestingcenters?) > > > > I think it is past time to stop the practical. So many of the stains > are done on machines. I have had a " registered tech," that did not > know how to run slides manually to just de-parffinize them. > > Why can't there just be more questions on the exam of theory ect. ect. > ect. > And that is my 2 cents worth....... > > Connie G. > >From: "Weems, Joyce" >To: "Madary, Joseph" > , >Subject: > RE: [Histonet] automated versus manual for > practical(practicaltestingcenters?) >Date: Tue, 5 Apr 2005 15:42:36 > -0400 > >Perhaps its time to stop the practical - do the other > disciplines do a practical? It's all so subjective. I think our > problem as a laboratory science is that we do not make diagnoses - > that sets us apart from the others. And some pathologists have > promoted the separation by not respecting us for how important we are > to them. My belief is that a pathologist is only as good as the tech. > Another 2 cents worth. > >Joyce > >Joyce Weems >Pathology Manager > >Saint Joseph's Hospital >5665 Peachtree Dunwoody Rd NE >Atlanta, GA > 30342 >404-851-7376 - Phone >404-851-7831 - Fax > >-----Original > Message----- >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of > Madary, >Joseph >Sent: Tuesday, April 05, 2005 3:12 PM >To: > histonet@lists.utsouthwestern.edu >Subject: [Histonet] automated > versus manual for practical(practical >testingcenters?) > > >As most I > did all three practicals manually getting the first in 1982. I have to > say though as a confirmed cheapskate what of the folks that rely on > the lab they are in to do the work and the supervisor will not approve > of purchasing reagents for manual, and even if the person paid, would > have an issue with the the regs/safety issues of getting new chemicals > in the lab? I am all for punishing new people in the lab and making > their life difficult espceially those non certified types, oh my God! > What if they have no access to manual methods? Maybe the folks using > automated methods should have to do more or something. How far does it > go, coverslipping? I mean one could argue buying a staining kit is > just as bad as automation since you are not` making reagents. I think > this is an important issue as you all have pointed out. Personally, I > feel there should be testing centers for the practical. Hey there is a > money making idea that someone can steal from me! > > >_______________________________________________ >Histonet mailing > list >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >Confidentiality Notice ** The information contained in this message > may be privileged and is confidential information intended for the use > of the addressee listed above. If you are neither the intended > recipient nor the employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > disclosure, copying, distribution or the taking of any action in > reliance on the contents of this information is strictly prohibited. > If you have received this communication in error, please notify us > immediately by replying to the message and deleting it from your > computer. >Thank you. Saint Josephs Health System, Inc. > > >_______________________________________________ >Histonet mailing > list >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DNolan <@t> evanhospital.com Wed Apr 6 13:46:26 2005 From: DNolan <@t> evanhospital.com (Donna M. Nolan) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] slides for Ventana Message-ID: <2C7B7A4D7DE62A46BC2EFB70D3ABF9326A4932@EVANXSCL.evanhospital.net> What brands have you tried and used successfully? I know others are less expensive but have been hesitant to try them and have something happen to the patient sample. From dellav <@t> musc.edu Wed Apr 6 14:15:41 2005 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] How to eliminate AFB contamination on slides? Message-ID: Heather, this can be a complex problem to resolve. acid fast organisms are environmental bugs and frequently contaminate water systems. we have lots of acid fast bugs in the tap water here. this means that your tissue float baths could have afb floating around in them, which can account for bugs being found on the glass surrounding sections that don't have the accompanying histologic features suggested of acid fast infection. don't believe me? it happened here and took so sleuthing to figure our where the contamination was coming from. we clean all tissue floats with bleach daily and allow to air dry after use. at another facility, we had a Millipore de-ionizing system with a 30 gallon wall mounted holding tank. the holding tank became contaminated with AFB which was a bit of a headache to clean. once again, changing out filters according to manufacturers recommendations is critical to avoiding this problem you will need to use ultra-filtered water using a sub-micron particle filter. be sure to change out these filters regularly as organisms can get past the filter once the filter becomes saturated. don't bother using sterile water. you have no assurance that bugs are not present in sterile water, just that they are non-viable. your carbol fuchsin stain solution can become contaminated from very positive control sections. it is important that slides are NOT immersed into a coplin jar of carbol fuchsin that you intend to re-use. slides should be stained horizontally, flooding the slides individually with stain. as has already been stated by others, do NOT place patient section onto the same slide as your positive control to avoid cross contamination good luck in resolving your problem Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> 04/06/05 01:34PM >>> We are having problems with the AFB stain. Almost everything we do AFB stains on has extracellular "AFB positive organisms" without an associated tissue response. Do we need to change our stain or use better grade water? Anybody who has any ideas or suggestions or has had this problem in the past, let me know what you did to correct the problem. Thank you to all in advance. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Wed Apr 6 14:25:30 2005 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] How to eliminate AFB contamination on slides? References: <807FE48C5A7CC940B973B58D32E7014318F93F63@nhpens-exch1.pcola.med.navy.mil> Message-ID: <002a01c53ade$66c4c750$7e034246@yourlk4rlmsu> If your method has sections in a coplin jar of carbol fuchsin in an oven at 60?C for an hour or so, then be aware that this technique has been shown to be a source of cross contamination. It's unfortunate since it produces good stains. Another source of contamination is the taps used for washing sections. They can have a scale built up inside the vacuum trap. The scale may be acid fast organisms. Eliminate by changing the vacuum trap and regularly cleaning the insides of the tap with a test tube brush. Bryan Llewellyn ----- Original Message ----- From: To: Sent: Wednesday, April 06, 2005 10:34 AM Subject: [Histonet] How to eliminate AFB contamination on slides? > We are having problems with the AFB stain. Almost everything we do AFB > stains on has extracellular "AFB positive organisms" without an associated > tissue response. Do we need to change our stain or use better grade water? > Anybody who has any ideas or suggestions or has had this problem in the > past, let me know what you did to correct the problem. Thank you to all in > advance. > > > > Heather A. Harper > > Supervisor of Histology/Morgue > > Naval Hospital of Pensacola, FL > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gcallis <@t> montana.edu Wed Apr 6 14:31:49 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] How to eliminate AFB contamination on slides? In-Reply-To: <807FE48C5A7CC940B973B58D32E7014318F93F63@nhpens-exch1.pcol a.med.navy.mil> References: <807FE48C5A7CC940B973B58D32E7014318F93F63@nhpens-exch1.pcola.med.navy.mil> Message-ID: <6.0.0.22.1.20050406132521.01bb6738@gemini.msu.montana.edu> Saprophytic acid fast organisms can grow in distilled water storage carboys, found in tubings on faucets and shower drains! Clean your distilled water containers frequently, use bleach, detergent and rinse well if you use carboys (polyethylene type). Set up a regular cleaning schedule to prevent contamination. Replace old tubings, etc on carboys, faucets regularly also. The organisms are not in your stain, more likely you get them when you pick up the section from water bath filled with contaminated water. Do you use tap water or distilled water in waterbath? If tap water, the tap may be the source. This is a known problem, there are publications about it. At 11:34 AM 4/6/2005, you wrote: >We are having problems with the AFB stain. Almost everything we do AFB >stains on has extracellular "AFB positive organisms" without an associated >tissue response. Do we need to change our stain or use better grade water? >Anybody who has any ideas or suggestions or has had this problem in the >past, let me know what you did to correct the problem. Thank you to all in >advance. > > > >Heather A. Harper > >Supervisor of Histology/Morgue > >Naval Hospital of Pensacola, FL > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From KMB1904 <@t> aol.com Wed Apr 6 14:51:59 2005 From: KMB1904 <@t> aol.com (KMB1904@aol.com) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] histotech wanted Message-ID: <66.54744e32.2f8597df@aol.com> We will be looking for a ASCP certified histotech at Nanticoke Memorial Hospital in Seaford, De. Our long time technician is retiring. It is a 2 person histology lab with 2 pathologists. Please reply if interested and I can give you the details! kmb1904@aol.com Kathy Bowden HT From David_S._Ahmed/HOU/UTMDACC <@t> mdanderson.org Wed Apr 6 15:29:36 2005 From: David_S._Ahmed/HOU/UTMDACC <@t> mdanderson.org (David_S._Ahmed/HOU/UTMDACC@mdanderson.org) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Histology Technician Opening Message-ID: M.D. Anderson Cancer Center in Houston,Texas has a full-time position open for a histology technician. If you are interested, please apply online at www.mdanderson.org. The contact person is Dianna Menard, Senior Recruiter, in Human Resources at (713)745-6184. Thank you. David S. Ahmed Chief Histology Technician Department of Pathology M.D. Anderson Cancer Center From miller <@t> coho.net Wed Apr 6 15:40:42 2005 From: miller <@t> coho.net (Diane G. Miller) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Clinical Lab References: <002901c53acb$9dcb84e0$7c01a8c0@biopath.org> Message-ID: <0bf801c53ae8$e7770d90$0200a8c0@desktop> Here is the listserver for the clinical side of the lab. http://www.ualberta.ca/~pletendr/MEDLAB-L.html Diane ----- Original Message ----- From: Paula Lucas To: Histonet (E-mail) Sent: Wednesday, April 06, 2005 10:11 AM Subject: [Histonet] Clinical Lab Does anyone know if there is a server like this one where people post questions to a group of subscribers in the clinical side of the lab (i.e. chemistry, blood, etc.). I know of someone who is looking at purchasing equipment for the clinical lab and she wants to get feedback from others who have experience with the various types that are out there. Thanks, Paula Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From claus <@t> itsa.ucsf.edu Wed Apr 6 16:04:51 2005 From: claus <@t> itsa.ucsf.edu (Claudia Schaller) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] beta galactosidase help! Message-ID: <42544EF3.1040902@itsa.ucsf.edu> Hi everybody, i need urgent help with beta galactosidase detection in Bouin's fixed testes sections (frozen and paraffin)! Testes got first fixed in Bouin's, then embedded in paraffin and O.C.T, respectively. Staining with X-gal on frozens didn't work? Does someone has any idea if the fixative (contains formaldehyde, picric acid, glacial acetic acid) destroys the enzyme? Does X-gal work on already paraffin embedded sections? Does someone know a good polyclonal anti beta- galactosidase antibody which i could use for the paraffin sections? ANY help is highly appreciated!!! Thanks Claudia From TJJ <@t> Stowers-Institute.org Wed Apr 6 16:34:17 2005 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Universal (protein) block for IHC Message-ID: Has anybody converted to using a universal protein block (casein) for their immunohistochemistry protocols rather than using species specific normal sera? For those who have made the conversion, did you have to make any changes to your methodology or did you just plug it in to your protocol and run? Did you notice any difference in the staining intensity for known antibodies (stronger, weaker, no change)? Are you also using it as a secondary antibody diluent or for washes? Finally, are you using commercially available reagent or are you making your own? Thanks so much! Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org From kelly.mcqueeney <@t> bms.com Wed Apr 6 16:41:39 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Universal (protein) block for IHC In-Reply-To: <0IEJ00120O1SKA@chimera.bms.com> References: <0IEJ00120O1SKA@chimera.bms.com> Message-ID: <42545793.6010107@bms.com> Hi Teri, I noticed a decrease in specific staining when I plugged it into my protocol. I made it myself and it was a big pain. Buy it! Try increasing antibody concentration on a few slides and see what happens. I never use a block with my secondary. Good luck! Johnson, Teri wrote: >Has anybody converted to using a universal protein block (casein) for >their immunohistochemistry protocols rather than using species specific >normal sera? For those who have made the conversion, did you have to >make any changes to your methodology or did you just plug it in to your >protocol and run? Did you notice any difference in the staining >intensity for known antibodies (stronger, weaker, no change)? Are you >also using it as a secondary antibody diluent or for washes? Finally, >are you using commercially available reagent or are you making your own? > >Thanks so much! > > >Teri Johnson >Managing Director Histology Facility >Stowers Institute for Medical Research >1000 E. 50th St. >Kansas City, Missouri 64110 >tjj@stowers-institute.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From katherine-walters <@t> uiowa.edu Wed Apr 6 17:13:36 2005 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Universal (protein) block for IHC Message-ID: I thought casein was just a fancy name for skim milk. I use it sometimes as an avidin block by making a 5% solution out of cheap dried milk. Kathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly D Mcqueeney Sent: Wednesday, April 06, 2005 4:42 PM To: Johnson, Teri Cc: Histonet Subject: Re: [Histonet] Universal (protein) block for IHC Hi Teri, I noticed a decrease in specific staining when I plugged it into my protocol. I made it myself and it was a big pain. Buy it! Try increasing antibody concentration on a few slides and see what happens. I never use a block with my secondary. Good luck! Johnson, Teri wrote: >Has anybody converted to using a universal protein block (casein) for >their immunohistochemistry protocols rather than using species specific >normal sera? For those who have made the conversion, did you have to >make any changes to your methodology or did you just plug it in to your >protocol and run? Did you notice any difference in the staining >intensity for known antibodies (stronger, weaker, no change)? Are you >also using it as a secondary antibody diluent or for washes? Finally, >are you using commercially available reagent or are you making your own? > >Thanks so much! > > >Teri Johnson >Managing Director Histology Facility >Stowers Institute for Medical Research >1000 E. 50th St. >Kansas City, Missouri 64110 >tjj@stowers-institute.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Apr 6 17:21:58 2005 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Universal (protein) block for IHC Message-ID: <000001c53af7$0c49e670$76d48a80@AMY> Terri I have been using dako's serum free protein block for about 1.5 years now. When I switched I just replaced that reagent with the blocking agent I was currently using. I have not noticed any decrease in staining intensity. I did see decreases in background staining, which could be due to the serum free protein block or improvement in my own technique, since I'm always learning about better ways to do things. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Wednesday, April 06, 2005 2:34 PM To: Histonet Subject: [Histonet] Universal (protein) block for IHC Has anybody converted to using a universal protein block (casein) for their immunohistochemistry protocols rather than using species specific normal sera? For those who have made the conversion, did you have to make any changes to your methodology or did you just plug it in to your protocol and run? Did you notice any difference in the staining intensity for known antibodies (stronger, weaker, no change)? Are you also using it as a secondary antibody diluent or for washes? Finally, are you using commercially available reagent or are you making your own? Thanks so much! Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Wed Apr 6 17:30:39 2005 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] 2 cents, 4cents, 10 cents, automated vs. manual... Message-ID: <1DB04B57B04C5747B87C3B39F3E605AA02E450FB@harrier> Basically have watched this thread evolve from ASCP power and policy (or lack of) issues etc. to the staining stuff. I've stayed out of the fray, yet wanted to comment. I may have forgotten a point or two along the way, but hope to make an intelligent contribution nonetheless. Thank you Peggy Wenk for directing folks to Marilyn Gamble and for the other links/information. Joe Nocito, et al; registry renewal and certification expiration could well be the product of a profit driven agenda. I realize that costs rise as time goes on. One could argue this is the price of higher technical standards. Maybe so, but it seems unnecessary to me. I believe it is the responsibility of the institution of employment to set standards for those they employ. Our staff techs have to be registry eligible (all are certified but one, who is scheduled to take the exam) and obtain 10 CEUs per year. I believe if people are not keeping up on CE they are devaluing themselves. They will soon find themselves dead-ended in a job or less than desirable for employment elsewhere. This is an example of individuals taking responsibility for themselves. I do not like the forced regulation, post-certification, as I believe this could be particularly detrimental to those who specialize. Charles Embrey, et al; thank you for the educational breakdown for histotechs and PAs. Charles, I am generally in agreement with you on most of your posts and I will state now that I do advocate education. Ian Montgomery, et al; thank you for your comments. I have met some very kind and intelligent colleagues from the UK. I have no doubt that there is more of a premium placed on advanced formal education in the UK than there is in the US. The nature and evolution of histology have gotten us to this point in which we are desperately trying to balance practical skill with advanced education. We are moving in the direction of education as what is expected of histotechs today has greatly expanded and diversified. This list-server alone is proof of that. We are becoming highly specialized and no one can know how to do it all and do it well. To my knowledge (US experience) historically, histology was initially performed by physicians or people off the street. This induced the development of professional, qualified and well educated non-physicians to handle the work. Of all lab disciplines (in my opinion) histology requires the greatest amount of eye-hand coordination and fine motor skill, along with an artistic ability. This is why there have been so many "good" non-registered histotechs out there in the field. A deft hand, some common sense and a good work ethic basically filled the bill. I have worked with some exceptional OJT techs. I have also worked with degreed people that have questionable functionality, so it goes. What it all boils down to is this; people need to know more for the sake of patients and the sake of themselves. Education is the only way. Candyce Fockler, et al; degrees are great, as I stated education is the key. I still believe in a practical demonstration of lab skill as an indicator of technician worth. Demonstration of solid technical skill by registry eligible individuals undoubtedly has merit. Certification only serves to validate that skill all the more. Manual versus automated - Tim Morken, et al; great points, I can appreciate the merits of manual staining (deeper understanding and all) hey, I'm an old school guy. But, automation is in the cards. With shortages and all we can't help it. Also, patient slides are submitted (real work) after automated staining for diagnosis. Tim (I believe) made the point that whether a stain is manual or automated it can still be goofed up. It's still up to the techs to know if it's right or not. The case of the tech and deparaffinizing slides is unbelievable. To me that is a serious educational gap that should not have been missed. I would like to thank everyone who has commented on this thread as it has evolved. I believe these are important matters to discuss and I have appreciated all viewpoints that have come out. I apologize to those I did not mention by name that made contributions, I thank you again as well. I don't believe this is tied into the thread but I would like to mention it now anyway. I hope that Julia Dahl and Stephen Peters will continue to post on this list server. They along with the other MDs (you know who you are) that monitor the Histonet are much needed to understand and further our profession. I sincerely appreciate the postings from these individuals. Thomas Jasper HT(ASCP)BAS Anatomic Pathology Coordinator SMDC Clinical Laboratory Duluth, MN tjasper@smdc.org This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From conniemoss <@t> relia.net Wed Apr 6 18:37:27 2005 From: conniemoss <@t> relia.net (Connie McManus) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist that we need to use Superfrost Plus s In-Reply-To: <1112721073.4252c6b15d0de@imp.vet.upenn.edu> References: <200504051702.j35H2Os0022864@chip.viawest.net> <1112721073.4252c6b15d0de@imp.vet.upenn.edu> Message-ID: <49765.208.186.240.165.1112830647.squirrel@email.relia.net> When we got the Ventana at the UT Vet Diag Lab, we were told not only by Ventana, but also by our partners at the NVSL that other brands produced irregularities in the staining. At the time, this seemed odd, so I decided to test things out just to see for myself. I used Surgipath, StatLab as well as Fisher's (and Erie Sci) and as far as I could tell, they all stained equally well, time after time. I never observed any staining irregularities. I have no idea what is behind this preference Ventana has with Fisher's. Frankly, I think it's carp fodder. In my opinion, you can choose which ever brand suits you, regardless of what Ventana says. -- Connie McManus Mt Ogden Scientific Services 950 W Kershaw, Suite E Ogden UT 84401 tel: 801/334-6677 direct: 801/745-2583 cell: 435/757/2975 fax: 435/514-1781 conniemoss@relia.net www.mtogdensci.com pmarcum@vet.upenn.edu said: > I believe the issue with Ventana saying only Plus slides from > Fisher or VWR is that these are all Erie slides and that is the > surface that holds best for the Ventana system. You may find slides > cheaper > however, check to see what the original manufacturer is so the sections > stay on the slides better. > > Pam Marcum > > Quoting Patsy Ruegg : > >> Statlabs has the best price for plus slides I have found. >> Patsy >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree >> Linda >> A. >> Sent: Tuesday, April 05, 2005 9:10 AM >> To: Angela Bitting; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XT >> andtheyinsist that we need to use Superfrost Plus s >> >> Angela, >> >> You will definitely need "Plus" slides no matter whose instrument you >> use. >> Find out which laboratory supply company your institution has a contract >> with. They may be your least expensive choice. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela >> Bitting >> Sent: Tuesday, April 05, 2005 10:31 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and >> theyinsist that we need to use Superfrost Plus s >> >> >> We're talking of buying a Ventana Benchmark XT and they insist that we >> need >> to use Superfrost Plus slides. They are going to cost much more than >> what >> we're using now. Does anyone have the inside track on the least >> expensive >> place to buy them from? You folks are always "The" >> source for All Histology Wisdom! Thanks! >> >> Angela Bitting, HT(ASCP) >> Technical Specialist, Histology >> Geisinger Medical Center >> 100 N Academy Ave. MC 23-00 >> Danville, PA 17822 >> phone 570-214-9634 >> fax 570-271-5916 >> >> >> IMPORTANT WARNING: The information in this message (and the documents >> attached to it, if any) is confidential and may be legally privileged. >> It is intended solely for the addressee. Access to this message by >> anyone else is unauthorized. If you are not the intended recipient, any >> disclosure, copying, distribution or any action taken, or omitted to be >> taken, in reliance on it is prohibited and may be unlawful. If you have >> received this message in error, please delete all electronic copies of >> this message (and the documents attached to it, if any), destroy any >> hard copies you may have created and notify me immediately by replying >> to this email. Thank you. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ====== From yelkaco <@t> ticon.net Wed Apr 6 19:05:30 2005 From: yelkaco <@t> ticon.net (Steven Coakley) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] information Message-ID: <004401c53b05$92df2520$8381b542@oemcomputer> Good afternoon, I am looking for Lee Lunas book Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. Also, does anyone know of a special stain used for differentiating mature and young collagen. Thank you, Steven C yelkaco@ticon.net From p_bourne_14526 <@t> yahoo.com Wed Apr 6 20:37:45 2005 From: p_bourne_14526 <@t> yahoo.com (Patricia Bourne) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist that we need to use Superfrost Plus s In-Reply-To: <49765.208.186.240.165.1112830647.squirrel@email.relia.net> Message-ID: <20050407013745.59359.qmail@web53703.mail.yahoo.com> We are testing the Ventana now and were told the same thing. We use Surgipath slides and have little if any problems. Interesting I had a call from Surgipath telling me they were discontinuing the slides I normally use and said since I used the Ventana the new slides they were getting would work better. I asked how did they know I even had a Ventana and they went silent. The silence says wonders.....I think the people Ventana are very interesting people...... __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From petepath <@t> yahoo.com Wed Apr 6 21:13:04 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] who reads what? Surg Path specimen distribution chart Message-ID: <20050407021304.51103.qmail@web30407.mail.mud.yahoo.com> I just uploaded a page describing the surg path specimen distribution list on my web site at: http://pathologyinnovations.com/surg_path_offerings.htm. I hope other find it as useful as our group has. Stephen Peters M.D. Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com Senior Attending Pathologist Hackensack University Medical Center 201 996 4836 From pruegg <@t> ihctech.net Wed Apr 6 21:18:36 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist that we need to use Superfrost Plus s In-Reply-To: <49765.208.186.240.165.1112830647.squirrel@email.relia.net> Message-ID: <200504070219.j372JK7g024270@pro12.abac.com> As Pam mentioned for some reason Ventana thinks that the slides produced by Erie hold the sections on better than any others, and perhaps that is true in their experience but I would suggest that trying other cheaper brands is worth an effort. I don't use the Ventana systems but I do lots of IHC and some very harsh HIER and I do not have a section detachment problem with the non-Erie + slides from StatLabs. Patsy -----Original Message----- From: Connie McManus [mailto:conniemoss@relia.net] Sent: Wednesday, April 06, 2005 4:37 PM To: pmarcum@vet.upenn.edu Cc: Patsy Ruegg; histonet@lists.utsouthwestern.edu; 'Sebree Linda A.' Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist that we need to use Superfrost Plus s When we got the Ventana at the UT Vet Diag Lab, we were told not only by Ventana, but also by our partners at the NVSL that other brands produced irregularities in the staining. At the time, this seemed odd, so I decided to test things out just to see for myself. I used Surgipath, StatLab as well as Fisher's (and Erie Sci) and as far as I could tell, they all stained equally well, time after time. I never observed any staining irregularities. I have no idea what is behind this preference Ventana has with Fisher's. Frankly, I think it's carp fodder. In my opinion, you can choose which ever brand suits you, regardless of what Ventana says. -- Connie McManus Mt Ogden Scientific Services 950 W Kershaw, Suite E Ogden UT 84401 tel: 801/334-6677 direct: 801/745-2583 cell: 435/757/2975 fax: 435/514-1781 conniemoss@relia.net www.mtogdensci.com pmarcum@vet.upenn.edu said: > I believe the issue with Ventana saying only Plus slides from > Fisher or VWR is that these are all Erie slides and that is the > surface that holds best for the Ventana system. You may find slides > cheaper > however, check to see what the original manufacturer is so the sections > stay on the slides better. > > Pam Marcum > > Quoting Patsy Ruegg : > >> Statlabs has the best price for plus slides I have found. >> Patsy >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree >> Linda >> A. >> Sent: Tuesday, April 05, 2005 9:10 AM >> To: Angela Bitting; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XT >> andtheyinsist that we need to use Superfrost Plus s >> >> Angela, >> >> You will definitely need "Plus" slides no matter whose instrument you >> use. >> Find out which laboratory supply company your institution has a contract >> with. They may be your least expensive choice. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela >> Bitting >> Sent: Tuesday, April 05, 2005 10:31 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and >> theyinsist that we need to use Superfrost Plus s >> >> >> We're talking of buying a Ventana Benchmark XT and they insist that we >> need >> to use Superfrost Plus slides. They are going to cost much more than >> what >> we're using now. Does anyone have the inside track on the least >> expensive >> place to buy them from? You folks are always "The" >> source for All Histology Wisdom! Thanks! >> >> Angela Bitting, HT(ASCP) >> Technical Specialist, Histology >> Geisinger Medical Center >> 100 N Academy Ave. MC 23-00 >> Danville, PA 17822 >> phone 570-214-9634 >> fax 570-271-5916 >> >> >> IMPORTANT WARNING: The information in this message (and the documents >> attached to it, if any) is confidential and may be legally privileged. >> It is intended solely for the addressee. Access to this message by >> anyone else is unauthorized. If you are not the intended recipient, any >> disclosure, copying, distribution or any action taken, or omitted to be >> taken, in reliance on it is prohibited and may be unlawful. If you have >> received this message in error, please delete all electronic copies of >> this message (and the documents attached to it, if any), destroy any >> hard copies you may have created and notify me immediately by replying >> to this email. Thank you. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ====== From petepath <@t> yahoo.com Wed Apr 6 21:29:35 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] who reads what? Surg Path specimen distribution chart Message-ID: <20050407022935.58300.qmail@web30404.mail.mud.yahoo.com> If you are having trouble with the link just go to my home page at www.pathologyinnovations.com and click on surg path offerings on the top navigation bar. From mab70 <@t> medschl.cam.ac.uk Thu Apr 7 01:58:30 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] information Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF21114D4@mius2.medlan.cam.ac.uk> I remember seeing something in Harry Cook's book. I can't remember at the moment but will look it up when I go to the lab. If I don't contact you in the next couple of days, remind me, you may contact me privately. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Steven Coakley [mailto:yelkaco@ticon.net] Sent: Thursday, April 07, 2005 1:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] information Good afternoon, I am looking for Lee Lunas book Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. Also, does anyone know of a special stain used for differentiating mature and young collagen. Thank you, Steven C yelkaco@ticon.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Krat18 <@t> aol.com Thu Apr 7 02:08:44 2005 From: Krat18 <@t> aol.com (Krat18@aol.com) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist th... Message-ID: <195.3c90aba5.2f86367c@aol.com> In a message dated 4/6/2005 9:20:39 P.M. Central Standard Time, pruegg@ihctech.net writes: I do not have a section detachment problem with the non-Erie + slides from StatLabs. I just want to add that I HAVE had a problem with sections detaching from the Surgipath + slides with my Ventana ..... very frustrating. Eliminated everything else as a problem, then switched back to Superfrost Plus, and now no problems at all. Guess that's why I wasn't too keen on trying other brands, since having sections fall off after a 3-1/2 hour immuno run was maddening to all of us, especially the pathologists! From ncoolen <@t> burns.nl Thu Apr 7 03:36:35 2005 From: ncoolen <@t> burns.nl (Coolen, Neeltje) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Laminin antibody Message-ID: <8A67A7CA4D84D44CB1B8F3D657CE599E300495@server-02.brandwonden.local> Dear Histonetters, Are there any people who have experience with the following anti-laminin antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)? We tried to use this antibody on paraffin-embedded human skin tissue (formalin-fixed), but it did not work. We tried several antigen retrieval procedures, like pepsin, proteinase K and trypsin digestion, but all of these fail. Does anyone know an appropriate antigen retrieval or should I try another antibody? Thank you for your help. Neeltje Coolen, PhD student VSBN Zeestraat 26 1941 AJ Beverwijk The Netherlands From David.Edmondson <@t> christie-tr.nwest.nhs.uk Thu Apr 7 06:06:15 2005 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Laminin antibody Message-ID: We use have used Protease digestion with success, though we do not use the Antibody much. Protease is Sigma P-8038, Type XXIV. 10mg in 10ml of PBS 20 mins Dave Histology Christie Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Coolen, Neeltje Sent: 07 April 2005 09:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Laminin antibody Dear Histonetters, Are there any people who have experience with the following anti-laminin antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)? We tried to use this antibody on paraffin-embedded human skin tissue (formalin-fixed), but it did not work. We tried several antigen retrieval procedures, like pepsin, proteinase K and trypsin digestion, but all of these fail. Does anyone know an appropriate antigen retrieval or should I try another antibody? Thank you for your help. Neeltje Coolen, PhD student VSBN Zeestraat 26 1941 AJ Beverwijk The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Thu Apr 7 08:00:54 2005 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Laminin antibody Message-ID: We're using a polyclonal laminin at 1:50 from Neomarkers with pretty good luck. Pretreatment with protease. The stain can be pretty dirty but we can't back off on the dilution or incubation time or we lose some of the specific staining. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Coolen, Neeltje Sent: Thursday, April 07, 2005 3:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Laminin antibody Dear Histonetters, Are there any people who have experience with the following anti-laminin antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)? We tried to use this antibody on paraffin-embedded human skin tissue (formalin-fixed), but it did not work. We tried several antigen retrieval procedures, like pepsin, proteinase K and trypsin digestion, but all of these fail. Does anyone know an appropriate antigen retrieval or should I try another antibody? Thank you for your help. Neeltje Coolen, PhD student VSBN Zeestraat 26 1941 AJ Beverwijk The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Thu Apr 7 08:02:07 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] We're talking of buying a Ventana BenchmarkXTandtheyinsist th... Message-ID: The issue is not detachment, it is stain repulsion. The extra charge on non-Erie slides tends to repel the stain away from the tissue when you use the Ventana systems with the liquid coverslip. It does not pose problems for other instruments and has nothing to do with tissue falling off. AND to further complicate things, other slides will work fine on the machines. It is only certain batches that leave Germany (yes 9 times out of 10 is German slides) with an extra amount of charge. So if you want to take the risk with other slides, go for it. I don't have time to repeat immunos, so if they recommend a particular slide I use them. Now lets get on with our lives. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Krat18@aol.com Sent: Thursday, April 07, 2005 2:09 AM To: pruegg@ihctech.net; conniemoss@relia.net; pmarcum@vet.upenn.edu Cc: histonet@lists.utsouthwestern.edu; la.sebree@hosp.wisc.edu Subject: Re: [Histonet] We're talking of buying a Ventana BenchmarkXTandtheyinsist th... In a message dated 4/6/2005 9:20:39 P.M. Central Standard Time, pruegg@ihctech.net writes: I do not have a section detachment problem with the non-Erie + slides from StatLabs. I just want to add that I HAVE had a problem with sections detaching from the Surgipath + slides with my Ventana ..... very frustrating. Eliminated everything else as a problem, then switched back to Superfrost Plus, and now no problems at all. Guess that's why I wasn't too keen on trying other brands, since having sections fall off after a 3-1/2 hour immuno run was maddening to all of us, especially the pathologists! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Thu Apr 7 08:09:27 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Laminin antibody Message-ID: We use the same antibody at 1:20 with proteinase k from DAKO for 10min at RT. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Coolen, Neeltje Sent: Thursday, April 07, 2005 3:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Laminin antibody Dear Histonetters, Are there any people who have experience with the following anti-laminin antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)? We tried to use this antibody on paraffin-embedded human skin tissue (formalin-fixed), but it did not work. We tried several antigen retrieval procedures, like pepsin, proteinase K and trypsin digestion, but all of these fail. Does anyone know an appropriate antigen retrieval or should I try another antibody? Thank you for your help. Neeltje Coolen, PhD student VSBN Zeestraat 26 1941 AJ Beverwijk The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu Apr 7 08:25:12 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Laminin antibody Message-ID: I use this antibody @ a 1:25 dilution. Dako LSAB2 detection. Dako Proteinase K enzyme retrieval for 5 minutes & it works very well. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Coolen, Neeltje [mailto:ncoolen@burns.nl] Sent: Thursday, April 07, 2005 2:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Laminin antibody Dear Histonetters, Are there any people who have experience with the following anti-laminin antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)? We tried to use this antibody on paraffin-embedded human skin tissue (formalin-fixed), but it did not work. We tried several antigen retrieval procedures, like pepsin, proteinase K and trypsin digestion, but all of these fail. Does anyone know an appropriate antigen retrieval or should I try another antibody? Thank you for your help. Neeltje Coolen, PhD student VSBN Zeestraat 26 1941 AJ Beverwijk The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kelly.mcqueeney <@t> bms.com Thu Apr 7 08:27:27 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:55 2005 Subject: [Histonet] Universal (protein) block for IHC In-Reply-To: References: Message-ID: <4255353F.2010603@bms.com> Casein is the predominant phosphoprotein (80%) found in fresh milk. It is relatively hydrophobic , making it poorly soluble in water. That is why I suggest buying it. Even though I did see a decrease in specific staining, it's probably due to my prep of casein. Manufacturers have developed the perfect concentration (0.3 or 0.03%, I forgot) for blocking. Best of luck! Walters, Katherine S wrote: >I thought casein was just a fancy name for skim milk. I use it >sometimes as an avidin block by making a 5% solution out of cheap dried >milk. > >Kathy > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly D >Mcqueeney >Sent: Wednesday, April 06, 2005 4:42 PM >To: Johnson, Teri >Cc: Histonet >Subject: Re: [Histonet] Universal (protein) block for IHC > >Hi Teri, >I noticed a decrease in specific staining when I plugged it into my >protocol. I made it myself and it was a big pain. Buy it! Try increasing > >antibody concentration on a few slides and see what happens. I never use > >a block with my secondary. > >Good luck! > >Johnson, Teri wrote: > > > >>Has anybody converted to using a universal protein block (casein) for >>their immunohistochemistry protocols rather than using species specific >>normal sera? For those who have made the conversion, did you have to >>make any changes to your methodology or did you just plug it in to your >>protocol and run? Did you notice any difference in the staining >>intensity for known antibodies (stronger, weaker, no change)? Are you >>also using it as a secondary antibody diluent or for washes? Finally, >>are you using commercially available reagent or are you making your >> >> >own? > > >>Thanks so much! >> >> >>Teri Johnson >>Managing Director Histology Facility >>Stowers Institute for Medical Research >>1000 E. 50th St. >>Kansas City, Missouri 64110 >>tjj@stowers-institute.org >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From JNocito <@t> Pathreflab.com Thu Apr 7 08:42:53 2005 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist that we need to use Superfrost Plus s In-Reply-To: <49765.208.186.240.165.1112830647.squirrel@email.relia.net> Message-ID: I have 2 Benchmark XT's and have had problems having tissue stay on the slides, especially from Surgipath. I talked to my rep and he said that for some reason, their slides do not work and they are working on a substitute. I purchase my plus slides from StatLab and don't have any problems. I agree with everyone. Doing immunos for 3 1/2 hours and then having the tissue fall off is irritating, not to mention expensive to repeat. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Connie McManus Sent: Wednesday, April 06, 2005 6:37 PM To: pmarcum@vet.upenn.edu Cc: histonet@lists.utsouthwestern.edu; 'Sebree Linda A.' Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist that we need to use Superfrost Plus s When we got the Ventana at the UT Vet Diag Lab, we were told not only by Ventana, but also by our partners at the NVSL that other brands produced irregularities in the staining. At the time, this seemed odd, so I decided to test things out just to see for myself. I used Surgipath, StatLab as well as Fisher's (and Erie Sci) and as far as I could tell, they all stained equally well, time after time. I never observed any staining irregularities. I have no idea what is behind this preference Ventana has with Fisher's. Frankly, I think it's carp fodder. In my opinion, you can choose which ever brand suits you, regardless of what Ventana says. -- Connie McManus Mt Ogden Scientific Services 950 W Kershaw, Suite E Ogden UT 84401 tel: 801/334-6677 direct: 801/745-2583 cell: 435/757/2975 fax: 435/514-1781 conniemoss@relia.net www.mtogdensci.com pmarcum@vet.upenn.edu said: > I believe the issue with Ventana saying only Plus slides from > Fisher or VWR is that these are all Erie slides and that is the > surface that holds best for the Ventana system. You may find slides > cheaper > however, check to see what the original manufacturer is so the sections > stay on the slides better. > > Pam Marcum > > Quoting Patsy Ruegg : > >> Statlabs has the best price for plus slides I have found. >> Patsy >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree >> Linda >> A. >> Sent: Tuesday, April 05, 2005 9:10 AM >> To: Angela Bitting; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XT >> andtheyinsist that we need to use Superfrost Plus s >> >> Angela, >> >> You will definitely need "Plus" slides no matter whose instrument you >> use. >> Find out which laboratory supply company your institution has a contract >> with. They may be your least expensive choice. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela >> Bitting >> Sent: Tuesday, April 05, 2005 10:31 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and >> theyinsist that we need to use Superfrost Plus s >> >> >> We're talking of buying a Ventana Benchmark XT and they insist that we >> need >> to use Superfrost Plus slides. They are going to cost much more than >> what >> we're using now. Does anyone have the inside track on the least >> expensive >> place to buy them from? You folks are always "The" >> source for All Histology Wisdom! Thanks! >> >> Angela Bitting, HT(ASCP) >> Technical Specialist, Histology >> Geisinger Medical Center >> 100 N Academy Ave. MC 23-00 >> Danville, PA 17822 >> phone 570-214-9634 >> fax 570-271-5916 >> >> >> IMPORTANT WARNING: The information in this message (and the documents >> attached to it, if any) is confidential and may be legally privileged. >> It is intended solely for the addressee. Access to this message by >> anyone else is unauthorized. If you are not the intended recipient, any >> disclosure, copying, distribution or any action taken, or omitted to be >> taken, in reliance on it is prohibited and may be unlawful. If you have >> received this message in error, please delete all electronic copies of >> this message (and the documents attached to it, if any), destroy any >> hard copies you may have created and notify me immediately by replying >> to this email. Thank you. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jackdodo <@t> msn.com Thu Apr 7 09:03:07 2005 From: jackdodo <@t> msn.com (WAYNE HOLLAND) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] nails (Can you charge) In-Reply-To: Message-ID: I am very interested in using this tween solution for nails only. I have a question about the charging. Since we will not be using an acid for the "Decal" Will we still be able to charge for a decal process? Would this still be considered a decal process, chemically speaking? Your thoughts PLEASE! >From: Dorothy.L.Webb@HealthPartners.Com >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] nails >Date: Tue, 05 Apr 2005 09:40:11 -0500 > > >There is a product from Sigma called "Tween" that works great at both >softening and fixation. It is a 5% solution in 10% formalin and stays in >there for 24 hours and then processed routinely, cut and placed on a >charged >slide and we only stain for PAS unless it has the nailbed with it. The >order number is P4634. Good luck! > > >________________________________________ > >This e-mail and any files transmitted with it are confidential and are >intended solely for the use of the individual or entity to whom they >are addressed. If you are not the intended recipient or the individual >responsible for delivering the e-mail to the intended recipient, please >be advised that you have received this e-mail in error and that any >use, dissemination, forwarding, printing, or copying of this e-mail >is strictly prohibited. > >If you have received this e-mail in error, please immediately notify >the HealthPartners Support Center by telephone at (952) 967-6600. >You will be reimbursed for reasonable costs incurred in notifying us. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tflore <@t> lsuhsc.edu Thu Apr 7 09:31:43 2005 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Does anyone charge for running immuno controls? Message-ID: Our EM Lab has never charged for controls on performing immunofluorescent studies. We do not use a negative and a positive control for each antigen (but I do understand that there is a new CAP regulation addressing this) but rather, our EM lab saves previously reported positive strongly positive for IgG, IgM, IgA, and C3. We section through leftover tissue (either renal or skin) and fix in hi grade acetone and save slides in a -70oc freezer. When sectioning a case for immunofluorescent studies, we pull two slides out. On one of the slides with control section we place patient section on the lower half of the control and label (+) and tag both patient and control tissue with Polyvalent (cocktail of IgG,IgM,IgA). The other control we label (-) and place a pt section on the lower part as well and leave in pbs and Coverslip. Does anyone charge for these controls. Our fee for immunofluorescent tagging is $100.00 each antigen. (I understand that the AFIP charges $157.00 for each antigen) Any imput will surely be appreciated. Teresa From juan.gutierrez <@t> christushealth.org Thu Apr 7 09:52:29 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Does anyone charge for running immuno controls? Message-ID: No we don't. Controls are only use for QC purposes. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Flores, Teresa Sent: Thursday, April 07, 2005 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does anyone charge for running immuno controls? Our EM Lab has never charged for controls on performing immunofluorescent studies. We do not use a negative and a positive control for each antigen (but I do understand that there is a new CAP regulation addressing this) but rather, our EM lab saves previously reported positive strongly positive for IgG, IgM, IgA, and C3. We section through leftover tissue (either renal or skin) and fix in hi grade acetone and save slides in a -70oc freezer. When sectioning a case for immunofluorescent studies, we pull two slides out. On one of the slides with control section we place patient section on the lower half of the control and label (+) and tag both patient and control tissue with Polyvalent (cocktail of IgG,IgM,IgA). The other control we label (-) and place a pt section on the lower part as well and leave in pbs and Coverslip. Does anyone charge for these controls. Our fee for immunofluorescent tagging is $100.00 each antigen. (I understand that the AFIP charges $157.00 for each antigen) Any imput will surely be appreciated. Teresa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Apr 7 09:57:21 2005 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Does anyone charge for running immuno controls? Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C63038BDD07@EMAIL.archildrens.org> We don't charge for controls. I would consider them part of the test. I've never heard of charging for them Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital - Changing Children's Lives Phone - 501.364.4240 Fax - 501.364.3912 Visit us on the web at www. archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Flores, Teresa Sent: Thursday, April 07, 2005 9:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does anyone charge for running immuno controls? Our EM Lab has never charged for controls on performing immunofluorescent studies. We do not use a negative and a positive control for each antigen (but I do understand that there is a new CAP regulation addressing this) but rather, our EM lab saves previously reported positive strongly positive for IgG, IgM, IgA, and C3. We section through leftover tissue (either renal or skin) and fix in hi grade acetone and save slides in a -70oc freezer. When sectioning a case for immunofluorescent studies, we pull two slides out. On one of the slides with control section we place patient section on the lower half of the control and label (+) and tag both patient and control tissue with Polyvalent (cocktail of IgG,IgM,IgA). The other control we label (-) and place a pt section on the lower part as well and leave in pbs and Coverslip. Does anyone charge for these controls. Our fee for immunofluorescent tagging is $100.00 each antigen. (I understand that the AFIP charges $157.00 for each antigen) Any imput will surely be appreciated. Teresa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From peoshel <@t> wisc.edu Thu Apr 7 10:00:52 2005 From: peoshel <@t> wisc.edu (Philip Oshel) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Universal (protein) block for IHC In-Reply-To: References: Message-ID: Kathy, Casein is the main protein in milk. Skim milk has lots more stuff in it than just casein, and who knows what that's doing to your sections? Phil >I thought casein was just a fancy name for skim milk. I use it >sometimes as an avidin block by making a 5% solution out of cheap dried >milk. > >Kathy > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly D >Mcqueeney >Sent: Wednesday, April 06, 2005 4:42 PM >To: Johnson, Teri >Cc: Histonet >Subject: Re: [Histonet] Universal (protein) block for IHC > >Hi Teri, >I noticed a decrease in specific staining when I plugged it into my >protocol. I made it myself and it was a big pain. Buy it! Try increasing > >antibody concentration on a few slides and see what happens. I never use > >a block with my secondary. > >Good luck! > >Johnson, Teri wrote: > >>Has anybody converted to using a universal protein block (casein) for >>their immunohistochemistry protocols rather than using species specific >>normal sera? For those who have made the conversion, did you have to >>make any changes to your methodology or did you just plug it in to your >>protocol and run? Did you notice any difference in the staining >>intensity for known antibodies (stronger, weaker, no change)? Are you >>also using it as a secondary antibody diluent or for washes? Finally, >>are you using commercially available reagent or are you making your >own? >> >>Thanks so much! >> >> >>Teri Johnson >>Managing Director Histology Facility >>Stowers Institute for Medical Research >>1000 E. 50th St. >>Kansas City, Missouri 64110 >>tjj@stowers-institute.org >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) From BMolinari <@t> heart.thi.tmc.edu Thu Apr 7 10:18:50 2005 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] GMA/VVG Message-ID: Hi all, A while ago a protocol was posted for a VVG (elastin)stain for GMA. I found the post but could not open the attachment. If anyone has a protocol for this stain I would greatly appreciate it. Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,Texas 77030 832-355-6524 832-355-6812 (fax) From dellav <@t> musc.edu Thu Apr 7 10:28:25 2005 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Universal (protein) block for IHC Message-ID: we use skim milk as our daily protein block and have done so for about two years.. it works fine and our immunos look great. I would recommend it as a suitable alternative. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> Philip Oshel 04/07/05 11:00AM >>> Kathy, Casein is the main protein in milk. Skim milk has lots more stuff in it than just casein, and who knows what that's doing to your sections? Phil >I thought casein was just a fancy name for skim milk. I use it >sometimes as an avidin block by making a 5% solution out of cheap dried >milk. > >Kathy > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly D >Mcqueeney >Sent: Wednesday, April 06, 2005 4:42 PM >To: Johnson, Teri >Cc: Histonet >Subject: Re: [Histonet] Universal (protein) block for IHC > >Hi Teri, >I noticed a decrease in specific staining when I plugged it into my >protocol. I made it myself and it was a big pain. Buy it! Try increasing > >antibody concentration on a few slides and see what happens. I never use > >a block with my secondary. > >Good luck! > >Johnson, Teri wrote: > >>Has anybody converted to using a universal protein block (casein) for >>their immunohistochemistry protocols rather than using species specific >>normal sera? For those who have made the conversion, did you have to >>make any changes to your methodology or did you just plug it in to your >>protocol and run? Did you notice any difference in the staining >>intensity for known antibodies (stronger, weaker, no change)? Are you >>also using it as a secondary antibody diluent or for washes? Finally, >>are you using commercially available reagent or are you making your >own? >> >>Thanks so much! >> >> >>Teri Johnson >>Managing Director Histology Facility >>Stowers Institute for Medical Research >>1000 E. 50th St. >>Kansas City, Missouri 64110 >>tjj@stowers-institute.org >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SJones <@t> cvm.tamu.edu Thu Apr 7 10:39:40 2005 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XT andtheyinsist that we need to use Supe Message-ID: I have found that the Surgipath plus coated slides that are manufactured by them (not an Erie slide) are more adhesive than the VWR slides. As far as I know, Surgipath is the only other manufacturer of plus coated slides besides Erie. Sarah Sarah Jones, HT(ASCP) Histology Lab Dept. of Veterinary Integrative Biosciences College of Veterinary Medicine Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> 04/05/05 12:11 PM >>> I believe the issue with Ventana saying only Plus slides from Fisher or VWR is that these are all Erie slides and that is the surface that holds best for the Ventana system. You may find slides cheaper however, check to see what the original manufacturer is so the sections stay on the slides better. Pam Marcum Quoting Patsy Ruegg : > Statlabs has the best price for plus slides I have found. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda > A. > Sent: Tuesday, April 05, 2005 9:10 AM > To: Angela Bitting; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XT > andtheyinsist that we need to use Superfrost Plus s > > Angela, > > You will definitely need "Plus" slides no matter whose instrument you use. > Find out which laboratory supply company your institution has a contract > with. They may be your least expensive choice. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela > Bitting > Sent: Tuesday, April 05, 2005 10:31 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and > theyinsist that we need to use Superfrost Plus s > > > We're talking of buying a Ventana Benchmark XT and they insist that we need > to use Superfrost Plus slides. They are going to cost much more than what > we're using now. Does anyone have the inside track on the least expensive > place to buy them from? You folks are always "The" > source for All Histology Wisdom! Thanks! > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. > It is intended solely for the addressee. Access to this message by > anyone else is unauthorized. If you are not the intended recipient, any > disclosure, copying, distribution or any action taken, or omitted to be > taken, in reliance on it is prohibited and may be unlawful. If you have > received this message in error, please delete all electronic copies of > this message (and the documents attached to it, if any), destroy any > hard copies you may have created and notify me immediately by replying > to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hborgeri <@t> wfubmc.edu Thu Apr 7 10:37:44 2005 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Universal (protein) block for IHC Message-ID: <9AEEF1FB6254224AA355ED285F8491650999CC26@EXCHVS2.medctr.ad.wfubmc.edu> I routinely use casein in both my diluent as well as wash buffer for all my immunos (I primarily use an alkaline phosphatase detection system, but when doing double or triple immuno labeling I also use an HRP or Beta Galactosidase system). Before converting from the standard protocol which used a non-immune serum from the same species as the primary for blocking non-specific proteins, I ran a comparison and found there to be no difference at all. I use a Tris wash buffer that contains 0.5% casein as well as 0.1% Tween 20 as a surfactant (I use the MicroProbe system which employs capillary action). My slides are clean and have virtually no background staining. The only time when I have noticed some non-specific binding is when using goat primaries. If anyone would like my protocol, please respond privately since I cannot attach on Histonet. Hermina Hermina M. Borgerink, BA, HTL(ASCP)QIHC Wake Forest University Health Sciences Department of Pathology Medical Center Blvd. Winston-Salem, NC 27157 Tel. (336) 716-1538 Fax. (336) 716-1515 e-mail hborgeri@wfubmc.edu "Treat a man as he appears to be, and you make him worse. But treat a man as if he already were what he potentially could be, and you make him what he should be." (Goethe) This electronic message, including any attachments, is confidential and proprietary and is solely for the intended recipient. If you are not the intended recipient, this message was sent to you in error and you are hereby advised that any review, disclosure, copying, distribution or use of this message, or any of the information included therein, is unauthorized and strictly prohibited. If you have received this electronic transmission in error, please immediately notify the sender by reply and permanently delete all copies of this message and its attachments. Thank you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Thursday, April 07, 2005 11:01 AM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Universal (protein) block for IHC Kathy, Casein is the main protein in milk. Skim milk has lots more stuff in it than just casein, and who knows what that's doing to your sections? Phil >I thought casein was just a fancy name for skim milk. I use it >sometimes as an avidin block by making a 5% solution out of cheap dried >milk. > >Kathy > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly D >Mcqueeney >Sent: Wednesday, April 06, 2005 4:42 PM >To: Johnson, Teri >Cc: Histonet >Subject: Re: [Histonet] Universal (protein) block for IHC > >Hi Teri, >I noticed a decrease in specific staining when I plugged it into my >protocol. I made it myself and it was a big pain. Buy it! Try increasing > >antibody concentration on a few slides and see what happens. I never use > >a block with my secondary. > >Good luck! > >Johnson, Teri wrote: > >>Has anybody converted to using a universal protein block (casein) for >>their immunohistochemistry protocols rather than using species specific >>normal sera? For those who have made the conversion, did you have to >>make any changes to your methodology or did you just plug it in to your >>protocol and run? Did you notice any difference in the staining >>intensity for known antibodies (stronger, weaker, no change)? Are you >>also using it as a secondary antibody diluent or for washes? Finally, >>are you using commercially available reagent or are you making your >own? >> >>Thanks so much! >> >> >>Teri Johnson >>Managing Director Histology Facility >>Stowers Institute for Medical Research >>1000 E. 50th St. >>Kansas City, Missouri 64110 >>tjj@stowers-institute.org >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Thu Apr 7 11:00:47 2005 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Re laminin AB Message-ID: <009101c53b8a$f692f940$112b5c9f@Carlos> I use Sigma's polyclonal Ab reagent....very good after HIER and more consistent and cleaner than proteolytic retrieval. See the protocol here http://www.immunoportal.com/ From pruegg <@t> ihctech.net Thu Apr 7 11:14:38 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist that we need to use Supe In-Reply-To: Message-ID: <200504071614.j37GEZs0005117@chip.viawest.net> Not so Sarah, StatLabs I am told have their + slides produced by someone in ?Germany. Patsy -----Original Message----- From: Sarah Jones [mailto:SJones@cvm.tamu.edu] Sent: Thursday, April 07, 2005 8:40 AM To: pruegg@ihctech.net; pmarcum@vet.upenn.edu Cc: la.sebree@hosp.wisc.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XTandtheyinsist that we need to use Supe I have found that the Surgipath plus coated slides that are manufactured by them (not an Erie slide) are more adhesive than the VWR slides. As far as I know, Surgipath is the only other manufacturer of plus coated slides besides Erie. Sarah Sarah Jones, HT(ASCP) Histology Lab Dept. of Veterinary Integrative Biosciences College of Veterinary Medicine Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> 04/05/05 12:11 PM >>> I believe the issue with Ventana saying only Plus slides from Fisher or VWR is that these are all Erie slides and that is the surface that holds best for the Ventana system. You may find slides cheaper however, check to see what the original manufacturer is so the sections stay on the slides better. Pam Marcum Quoting Patsy Ruegg : > Statlabs has the best price for plus slides I have found. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda > A. > Sent: Tuesday, April 05, 2005 9:10 AM > To: Angela Bitting; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] We're talking of buying a Ventana Benchmark XT > andtheyinsist that we need to use Superfrost Plus s > > Angela, > > You will definitely need "Plus" slides no matter whose instrument you use. > Find out which laboratory supply company your institution has a contract > with. They may be your least expensive choice. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela > Bitting > Sent: Tuesday, April 05, 2005 10:31 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] We're talking of buying a Ventana Benchmark XT and > theyinsist that we need to use Superfrost Plus s > > > We're talking of buying a Ventana Benchmark XT and they insist that we need > to use Superfrost Plus slides. They are going to cost much more than what > we're using now. Does anyone have the inside track on the least expensive > place to buy them from? You folks are always "The" > source for All Histology Wisdom! Thanks! > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. > It is intended solely for the addressee. Access to this message by > anyone else is unauthorized. If you are not the intended recipient, any > disclosure, copying, distribution or any action taken, or omitted to be > taken, in reliance on it is prohibited and may be unlawful. If you have > received this message in error, please delete all electronic copies of > this message (and the documents attached to it, if any), destroy any > hard copies you may have created and notify me immediately by replying > to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu Apr 7 11:19:54 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Ethyl Alcohol Message-ID: All, Routine histology in my lab is looking for alternative sources for their ethanol. They currently use a distilled ethanol that is NOT denatured which they order from AAPER. The box reads "200 proof USP Ethyl Alcohol". She may be superstitious but the histology manager does not want to change to something like a denatured ethanol or dehydrants containing multiple types of alcohols so she wants "the real thing". Blocks are coming out just as she wants them to so she would be loathe to change anything on the tissue processors. I believe she needs a permit to have this kind of ethanol but sees that as a necessary evil. A recent drastic price hike has her searching for alternatives. Any suggestions would be very welcome. Thanx in Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From settembr <@t> umdnj.edu Thu Apr 7 11:37:40 2005 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Laminin antibody Message-ID: Neeltje, Try the antibody with no antigen retreival. Dana Settembre University Hospital -UMDNJ Newark, NJ USA >>> "Coolen, Neeltje" 4/7/2005 4:36:35 AM >>> Dear Histonetters, Are there any people who have experience with the following anti-laminin antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)? We tried to use this antibody on paraffin-embedded human skin tissue (formalin-fixed), but it did not work. We tried several antigen retrieval procedures, like pepsin, proteinase K and trypsin digestion, but all of these fail. Does anyone know an appropriate antigen retrieval or should I try another antibody? Thank you for your help. Neeltje Coolen, PhD student VSBN Zeestraat 26 1941 AJ Beverwijk The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Thu Apr 7 11:50:42 2005 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] GMA/VVG Message-ID: Sorry, elastic -----Original Message----- From: Lucy Brooks [mailto:lucyb@biocare.net] Sent: Thursday, April 07, 2005 10:29 AM To: Molinari, Betsy Subject: Re: [Histonet] GMA/VVG Elastin or Elastic?? ----- Original Message ----- From: "Molinari, Betsy" To: Sent: Thursday, April 07, 2005 8:18 AM Subject: [Histonet] GMA/VVG Hi all, A while ago a protocol was posted for a VVG (elastin)stain for GMA. I found the post but could not open the attachment. If anyone has a protocol for this stain I would greatly appreciate it. Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,Texas 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bjrenquist <@t> ucdavis.edu Thu Apr 7 12:14:43 2005 From: bjrenquist <@t> ucdavis.edu (Ben) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Thanks on the cryopreservative question. Message-ID: <200504071714.j37HEiOC018854@pop19.ucdavis.edu> Thanks to all that have responded. You have been a great help. I'm going to infiltrate the block with 30% sucrose without the ethylene glycol then store the slices in the ethylene glycol cryopreservative at -20C. Looks like the best course of action. Ben Here is the thread of messages on ethylene glycol cryoprotectant: Hello All, I am attempting to slice sheep brainstem and hypothalamus on a cryostat at -20 C. The tissue was treated as such: Perfusion through carotid artery (6L of 4% paraformaldehyde) Postfixation for 24 hours at 4 C Cryoprotected in a 20% sucrose solution for 5 days at 4 C Cryoprotected in a 30% sucrose 30% ethylene glycol solution and stored at -20 C. I am having some major problems with slicing this tissue. It appears to never freeze. Does anyone know at what temperature I should be slicing? Any opinions on use of a vibratome instead of a cryostat? I'm trying to section tissue at 30-40 um. Thanks in advance for your help. Ben Renquist UCDavis Response 1: Ethylene Glycol is anti-freeze. Pam Marcum Response 2: This can be answer. The 30% ethylene glycol solution is your problem. Next time do it without ethylene glycol. 20% sucrose following by 30% sucrose is good enough to protect the tissue. Good luck, Margaryan Response 3: Ethylene glycol is anti-freeze - don't use it. Just let brain cryoprotect in 30% sucrose - we never bother with a sucrose gradient anymore but you can use it if you want. You should snap freeze the brain properly and then get good frozen sections at that thickness at approx -17C. but if you have anti-freeze in tissue, you are probably cutting mush at that temperature. Response 4: Ben, The only time I have seen or used the ethylene glycol in sucrose as a cryoprotectant has been on brains that have been cut on a sliding microtome (using dry ice to freeze the tissue), for thick sections (30 microns +), for floating sections. The sections collapse on the knife, are picked up with a paint brush, and flattened out in PBS in the well plate for staining before mounting onto the slide. James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Response 5: I also had some problems sectioning rat brain in the cryostat after perfusion. I got a lot of folding and curling (always 20 micron). This may be due to the temperature in the cryostat. I sectioned the fixed tissue at the same temperature we section fresh tissue. We section fresh tissue at knife = -13, specimen = -8 with one cryostat and knife = -15, specimen = -11 with our other cryostat. The optimal cutting temperature always varies from one cryostat to another, it is important to play around with the temperature if you are getting folding or cracking.. Gayle, do you use -17C for specimen and knife (chamber) temperature? Thanks, Kelly Question to Responders 2 and 3: Thank you for your response. I already have some tissue that I would like to stain. Is it possible to pull out the ethylene glycol by putting into 30% sucrose and changing that out every few days to maintain the ethylene glycol gradient. Ben Response 6: You can try. I am not sure. Ask our folks. Good luck Response 7: I would just immerse in 30% sucrose, it will exchange without a gradient, just get rid of the ethylene glycol as fast as possible. This whole storage of sucrose cryoprotected tissue problem was just discussed. You can store in 30% sucrose at 4C until you need to snap freeze and avoid storing at -20C. I don't recall people using ethylene glycol at all. the tissue IS fixed, and I don't think anything grow in 30% sucrose Or snap freeze your samples after 30% sucrose cryoprotection, and store frozen blocks at -80C, this is what we do, until you need to equilibrate to cutting temperatures. I would not want even a trace of ethylene glycol contamination around any of my tissues - I avoid alcohol fixatives for this reason too. Fiddling with using EG gradient, then undoing it is a pain and a lot of unnecessary work plus it is messing up your frozen sections. I do not recall ever seeing EG used for this purpose - is this something you have as a protocol? if so, from where? Gayle Callis Question to Responder 6: Thanks, one more question, then I'll quit bothering you. Is there any chance that a vibratome might work on this tissue or would I have similar problems with that? Ben Response 8: I think it can, I usually do agarose embedded tissue for vibratome, but I think it can also work. Please, let me know when you will finish: if it worked? All best, Naira From kgibbon <@t> qltinc.com Thu Apr 7 12:16:43 2005 From: kgibbon <@t> qltinc.com (kgibbon@qltinc.com) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Ethyl Alcohol Message-ID: hi Glen, I have used denatured alcohol for many years for all processing and dehydrating. Most of the vendors sell it in various container sizes, the larger the cheaper usually. I have no problems with any stains or tissues. It is too much hassle to fill in the customs duty forms for the pure stuff Kevin Gibbon QLT Inc. |---------+-----------------------------------------> | | "Dawson, Glen" | | | | | | Sent by: | | | histonet-bounces@lists.utsouth| | | western.edu | | | | | | | | | 04/07/2005 09:19 AM | | | | |---------+-----------------------------------------> >------------------------------------------------------------------------------------------------------------------------------| | | | To: histonet@lists.utsouthwestern.edu | | cc: | | Subject: [Histonet] Ethyl Alcohol | >------------------------------------------------------------------------------------------------------------------------------| All, Routine histology in my lab is looking for alternative sources for their ethanol. They currently use a distilled ethanol that is NOT denatured which they order from AAPER. The box reads "200 proof USP Ethyl Alcohol". She may be superstitious but the histology manager does not want to change to something like a denatured ethanol or dehydrants containing multiple types of alcohols so she wants "the real thing". Blocks are coming out just as she wants them to so she would be loathe to change anything on the tissue processors. I believe she needs a permit to have this kind of ethanol but sees that as a necessary evil. A recent drastic price hike has her searching for alternatives. Any suggestions would be very welcome. Thanx in Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Apr 7 13:07:47 2005 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Universal (protein) block for IHC Message-ID: I find it interesting that some labs still use a protein or normal sera block. We stopped doing this years ago (for paraffin sections) and we never see any nonspecific staining. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Johnson, Teri" 04/06/05 05:34PM >>> Has anybody converted to using a universal protein block (casein) for their immunohistochemistry protocols rather than using species specific normal sera? For those who have made the conversion, did you have to make any changes to your methodology or did you just plug it in to your protocol and run? Did you notice any difference in the staining intensity for known antibodies (stronger, weaker, no change)? Are you also using it as a secondary antibody diluent or for washes? Finally, are you using commercially available reagent or are you making your own? Thanks so much! Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------- Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From liz <@t> premierlab.com Thu Apr 7 13:09:41 2005 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] plus slides Message-ID: <001201c53b9c$f85f0180$76d48a80@AMY> For all this is worth, here is my experience with plus slides. I have found no differences in tissue attachment in the following brands of plus slides - VWR, Fisher, Stat Labs superfrost plus and histobond slides - I would use any one of these for immuno's without fear of losing my sections. I have tried Surgipaths snowcoat x-tra and some plus slide that Mercedes Medical sells (made in China). In my experience the immuno's I did with the slides from surgipath and Mercedes medical, the tissue sections fell of the slides (with both automated and very careful handling of manual methods) I would never use those slides for immunos or even fixed frozen sections. I cut some fixed tissue for frozen and used surgipaths snowcoat extra and the sections fell off, but adhered just fine to the the StatLab histobond slides. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 From djohnson14 <@t> hotmail.com Thu Apr 7 13:29:50 2005 From: djohnson14 <@t> hotmail.com (Dave Johnson) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] plus slides In-Reply-To: <001201c53b9c$f85f0180$76d48a80@AMY> Message-ID: What about the Mercedes Medical Starfrost Plus german slides? >From: "Elizabeth Chlipala" >To: "'Histonet'" >Subject: [Histonet] plus slides >Date: Thu, 7 Apr 2005 12:09:41 -0600 > >For all this is worth, here is my experience with plus slides. I have >found no differences in tissue attachment in the following brands of >plus slides - > >VWR, Fisher, Stat Labs superfrost plus and histobond slides - I would >use any one of these for immuno's without fear of losing my sections. > >I have tried Surgipaths snowcoat x-tra and some plus slide that Mercedes >Medical sells (made in China). In my experience the immuno's I did with >the slides from surgipath and Mercedes medical, the tissue sections fell >of the slides (with both automated and very careful handling of manual >methods) I would never use those slides for immunos or even fixed >frozen sections. I cut some fixed tissue for frozen and used surgipaths >snowcoat extra and the sections fell off, but adhered just fine to the >the StatLab histobond slides. > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >Manager >Premier Laboratory, LLC >P.O. Box 18592 >Boulder, Colorado 80308 >Office: (303) 735-5001 >Fax: (303) 735-3540 >liz@premierlab.com >www.premierlab.com > >Ship to Address: >Premier Laboratory >University of Colorado >MCDB, Room A3B40 >Boulder, Colorado 80309 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Thu Apr 7 13:41:02 2005 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Universal (protein) block for IHC Message-ID: For antibody staining in clinical labs, antibodies and detection systems have been well characterized and optimized for use in human samples. When we automated the immunostaining in the clinical labs I worked in, we never used it there either. Performing antibody staining on animal samples, especially polyclonal antibody staining, tends to bring more background into the picture. Unfortunately there are times when we have very small amounts of sample (be it antibody or specimen) to do our initial workups, and therefore really need to start with a system that gives us the greatest chance for success with little to no background staining. When using any avidin/biotin system, we routinely do a biotin block. We know not every tissue sample will have endogenous biotin causing non-specific staining, but it just keeps us from having to include a control to account for that variable alone. Same goes with the protein block. Undoubtedly, many of us continue to do things in protocols "because we've always done it". When trying to obtain staining using antibodies which may have never been tested in IHC against species also never tested, which we do a lot of, I tend to lean toward the side of what can theoretically produce the best possible outcome the first time out of the gates. We would need to do some serious side-by-side in-house development for me to be comfortable dropping it altogether in this environment. Saw a quote that I dearly love--don't know who to attribute it to: "I want to live in Theory--everything works there." Best wishes, Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Thursday, April 07, 2005 1:08 PM To: histonet@lists.utsouthwestern.edu; Johnson, Teri Subject: Re: [Histonet] Universal (protein) block for IHC I find it interesting that some labs still use a protein or normal sera block. We stopped doing this years ago (for paraffin sections) and we never see any nonspecific staining. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Johnson, Teri" 04/06/05 05:34PM >>> Has anybody converted to using a universal protein block (casein) for their immunohistochemistry protocols rather than using species specific normal sera? For those who have made the conversion, did you have to make any changes to your methodology or did you just plug it in to your protocol and run? Did you notice any difference in the staining intensity for known antibodies (stronger, weaker, no change)? Are you also using it as a secondary antibody diluent or for washes? Finally, are you using commercially available reagent or are you making your own? Thanks so much! Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------- Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From dellav <@t> musc.edu Thu Apr 7 13:49:27 2005 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] plus slides Message-ID: we use these daily without problems. >>> "Dave Johnson" 04/07/05 02:29PM >>> What about the Mercedes Medical Starfrost Plus german slides? >From: "Elizabeth Chlipala" >To: "'Histonet'" >Subject: [Histonet] plus slides >Date: Thu, 7 Apr 2005 12:09:41 -0600 > >For all this is worth, here is my experience with plus slides. I have >found no differences in tissue attachment in the following brands of >plus slides - > >VWR, Fisher, Stat Labs superfrost plus and histobond slides - I would >use any one of these for immuno's without fear of losing my sections. > >I have tried Surgipaths snowcoat x-tra and some plus slide that Mercedes >Medical sells (made in China). In my experience the immuno's I did with >the slides from surgipath and Mercedes medical, the tissue sections fell >of the slides (with both automated and very careful handling of manual >methods) I would never use those slides for immunos or even fixed >frozen sections. I cut some fixed tissue for frozen and used surgipaths >snowcoat extra and the sections fell off, but adhered just fine to the >the StatLab histobond slides. > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >Manager >Premier Laboratory, LLC >P.O. Box 18592 >Boulder, Colorado 80308 >Office: (303) 735-5001 >Fax: (303) 735-3540 >liz@premierlab.com >www.premierlab.com > >Ship to Address: >Premier Laboratory >University of Colorado >MCDB, Room A3B40 >Boulder, Colorado 80309 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Thu Apr 7 13:55:52 2005 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Universal (protein) block for IHC Message-ID: Just like Richard Cartun's lab, we also stopped using normal sera/ protein block years ago with consistently good results. Dana Settembre University Hospital-UMDNJ Newerk, NJ >>> Richard Cartun 4/7/2005 2:07:47 PM >>> I find it interesting that some labs still use a protein or normal sera block. We stopped doing this years ago (for paraffin sections) and we never see any nonspecific staining. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Johnson, Teri" 04/06/05 05:34PM >>> Has anybody converted to using a universal protein block (casein) for their immunohistochemistry protocols rather than using species specific normal sera? For those who have made the conversion, did you have to make any changes to your methodology or did you just plug it in to your protocol and run? Did you notice any difference in the staining intensity for known antibodies (stronger, weaker, no change)? Are you also using it as a secondary antibody diluent or for washes? Finally, are you using commercially available reagent or are you making your own? Thanks so much! Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------- Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thallada <@t> noch.org Thu Apr 7 14:35:30 2005 From: thallada <@t> noch.org (Hallada, Teri) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Cryostat disposable blades Message-ID: I have finally convinced our pathologists to try disposable blades in the cryostat. I would like to hear from the group about any favorite blades or any other helpful hints. Teri Hallada BS MT CT (ASCP) thallada@noch.org Confidentiality Statement: This message is intended only for the individual or entity to which it is addressed. It may contain privileged, confidential information which is exempt from disclosure under applicable laws. If you are not the intended recipient, please note that you are strictly prohibited from disseminating or distributing this information (other than to the intended recipient) or copying this information. If you have received this communication in error, please notify us immediately by e-mail or by telephone at (616) 842-3600. Thank you. From DRG <@t> Stowers-Institute.org Thu Apr 7 14:47:31 2005 From: DRG <@t> Stowers-Institute.org (Grant, Debra) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Cryostat disposable blades Message-ID: Hi Teri, We use Accu-Edge high profile from Sakura for our Leica and Microm cryostat. We also use the same disposable blades for our Leica and Microm automated microtomes. They work great! Debby Grant Histology Specialist I Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hallada, Teri Sent: Thursday, April 07, 2005 2:36 PM To: Histonet Subject: [Histonet] Cryostat disposable blades I have finally convinced our pathologists to try disposable blades in the cryostat. I would like to hear from the group about any favorite blades or any other helpful hints. Teri Hallada BS MT CT (ASCP) thallada@noch.org Confidentiality Statement: This message is intended only for the individual or entity to which it is addressed. It may contain privileged, confidential information which is exempt from disclosure under applicable laws. If you are not the intended recipient, please note that you are strictly prohibited from disseminating or distributing this information (other than to the intended recipient) or copying this information. If you have received this communication in error, please notify us immediately by e-mail or by telephone at (616) 842-3600. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From browning <@t> HHSC.CA Thu Apr 7 15:12:36 2005 From: browning <@t> HHSC.CA (Browning Deb) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Universal (protein) block for IHC Message-ID: <3AADFB88753AD31189C100902786B91C17919E43@hch_nt_exchange.hhsc.ca> May I ask, what do you use to block, or is this step skipped altogether? There was a time we couldn't use a blocker because it reacted with the antibody diluent, so we actually saved time. Now we have switched to kits that come with blocker, so we use it, with no adverse effect other than increased TAT. -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Thursday, April 07, 2005 2:08 PM To: histonet@lists.utsouthwestern.edu; TJJ@Stowers-Institute.org Subject: Re: [Histonet] Universal (protein) block for IHC I find it interesting that some labs still use a protein or normal sera block. We stopped doing this years ago (for paraffin sections) and we never see any nonspecific staining. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Johnson, Teri" 04/06/05 05:34PM >>> Has anybody converted to using a universal protein block (casein) for their immunohistochemistry protocols rather than using species specific normal sera? For those who have made the conversion, did you have to make any changes to your methodology or did you just plug it in to your protocol and run? Did you notice any difference in the staining intensity for known antibodies (stronger, weaker, no change)? Are you also using it as a secondary antibody diluent or for washes? Finally, are you using commercially available reagent or are you making your own? Thanks so much! Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------- Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From petepath <@t> yahoo.com Thu Apr 7 15:18:50 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Cryostat disposable blades Message-ID: <20050407201850.70862.qmail@web30403.mail.mud.yahoo.com> We use accu edge and have found them to work well. I could not resist offering this excerpt from my frozen section tutorial. I find that I always get my best quality section with a new sharp blade. I think some of the places we try to save money in medicine are a bit "pound foolish". Your patients surgery is costing thousands of dollars. Hundreds of dollars are spent on disposables including lap pads, gloves, sponges, drapes, cautery, needles, needle magnets, BP cuff, IV tubing, ……..We are conserving pennies on what may be the most important decision impacting on the procedure. Yet some will risk quality by trying to get "20 shaves" out of a disposable blade. In my practice I treat every patient to a new section of blade. I will change it as soon as my section quality begins to fall. Some tissues such as tough collagenous tissues or calcified tissues will quickly dull the blade. If I'm having trouble getting a good section with a new blade on occasion I have changed to a second new blade and easily prepared a quality section. Safety Tip If you cut yourself on a blade used on only one patient, you will have minimized your risk of transmittable disease. If you cut yourself on a blade that has been used for days, it is like sleeping with numerous partners......without the fun! In this day and age of doing FNA's with ridiculously flexible long safety needles and using annoying safety scalpels we can justify using a sharp blade for safety reasons..... and get the benefit of awesome frozens every time! From mab70 <@t> medschl.cam.ac.uk Fri Apr 8 02:22:57 2005 From: mab70 <@t> medschl.cam.ac.uk (Margaret Blount) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Cryostat disposable blades Message-ID: <2A70D44ECF6F1A4390DD1D98E8BEDEF21114D6@mius2.medlan.cam.ac.uk> I like Feather C35 blades, they are very reliable. Margaret Margaret Blount Chief Technician Clinical Biochemistry University of Cambridge Addenbrooke's Hospital Hills Road Cambridge CB2 2QR -----Original Message----- From: Hallada, Teri [mailto:thallada@noch.org] Sent: Thursday, April 07, 2005 8:36 PM To: Histonet Subject: [Histonet] Cryostat disposable blades I have finally convinced our pathologists to try disposable blades in the cryostat. I would like to hear from the group about any favorite blades or any other helpful hints. Teri Hallada BS MT CT (ASCP) thallada@noch.org Confidentiality Statement: This message is intended only for the individual or entity to which it is addressed. It may contain privileged, confidential information which is exempt from disclosure under applicable laws. If you are not the intended recipient, please note that you are strictly prohibited from disseminating or distributing this information (other than to the intended recipient) or copying this information. If you have received this communication in error, please notify us immediately by e-mail or by telephone at (616) 842-3600. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ctsblack <@t> capeheart.uct.ac.za Fri Apr 8 03:03:17 2005 From: ctsblack <@t> capeheart.uct.ac.za (Melanie Black) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Smoothelin Antibody from Abcam Message-ID: Hi There Perhaps somebody could help me with my problem?? I am trying to differentiate Myofibroblasts from Smooth Muscle cells. SMC's will obviously stain positive with Actin,Desmin, myosin and Vimentin. I want to do a Smoothelin stain, for which Myofibroblasts are negative and thereby assume that I have identified Myo's. I am using Abcam's Anti- Smoothelin, and using placenta and tonsil controls trying to optimize this Ab. However....it is staining TROPHOBLASTS and NOT Smooth Muscle Cells at all!!!!! I am getting stunning Specific staining of the entire placental Trophoblasts, and nothing on contrcatile vessels/arteries. Has anybody used this antibody at all????? AB 8969 Anti- Smoothelin. I would appreciate any adivice. Many Thanks Melanie Black PS: I have referred this onto Abcam Technical Support and am waiting for a reply. -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 From ctsblack <@t> capeheart.uct.ac.za Fri Apr 8 03:28:56 2005 From: ctsblack <@t> capeheart.uct.ac.za (Melanie Black) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Smoothelin Antibody from Abcam Message-ID: Hi There Perhaps somebody could help me with my problem?? I am trying to differentiate Myofibroblasts from Smooth Muscle cells. SMC's will obviously stain positive with Actin,Desmin, myosin and Vimentin. I want to do a Smoothelin stain, for which Myofibroblasts are negative and thereby assume that I have identified Myo's. I am using Abcam's Anti- Smoothelin, and using placenta and tonsil controls trying to optimize this Ab. However....it is staining TROPHOBLASTS and NOT Smooth Muscle Cells at all!!!!! I am getting stunning Specific staining of the entire placental Trophoblasts, and nothing on contrcatile vessels/arteries. Has anybody used this antibody at all????? AB 8969 Anti- Smoothelin. I would appreciate any adivice. Many Thanks Melanie Black PS: I have referred this onto Abcam Technical Support and am waiting for a reply. -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 From ctsblack <@t> capeheart.uct.ac.za Fri Apr 8 05:00:28 2005 From: ctsblack <@t> capeheart.uct.ac.za (Melanie Black) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Smoothelin Antibody from Abcam Message-ID: Hi There Perhaps somebody could help me with my problem?? I am trying to differentiate Myofibroblasts from Smooth Muscle cells. SMC's will obviously stain positive with Actin,Desmin, myosin and Vimentin. I want to do a Smoothelin stain, for which Myofibroblasts are negative and thereby assume that I have identified Myo's. I am using Abcam's Anti- Smoothelin, and using placenta and tonsil controls trying to optimize this Ab. However....it is staining TROPHOBLASTS and NOT Smooth Muscle Cells at all!!!!! I am getting stunning Specific staining of the entire placental Trophoblasts, and nothing on contrcatile vessels/arteries. Has anybody used this antibody at all????? AB 8969 Anti- Smoothelin. I would appreciate any adivice. Many Thanks Melanie Black PS: I have referred this onto Abcam Technical Support and am waiting for a reply. -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 From DNolan <@t> evanhospital.com Fri Apr 8 05:13:10 2005 From: DNolan <@t> evanhospital.com (Donna M. Nolan) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] slides for Ventana Message-ID: <2C7B7A4D7DE62A46BC2EFB70D3ABF9326A4935@EVANXSCL.evanhospital.net> We have been using the colorfrost slides for a year but have been having trouble with the sections lifting on the Ventana Benchmark. I thought it might be the way we pick the section up from the water bath but we tried the SurgiPath slides the other day and those sections stayed on just fine. The SurgiPath slides we purchased seem to hold the specimen better.. We will have to evaluate the staining. We have tried both slides for H&E staining when the tissue is hard and the SurgiPath usually is the one the tissue stays on. From akbitting <@t> geisinger.edu Fri Apr 8 06:57:19 2005 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] plus slides Message-ID: Again, the issue is not with tissue adhesion, it is the uniformity of the coating. The XT uses air to mix the reagent while its on the slides and if the coating isn't uniform there is spotty staining. >>> "Elizabeth Chlipala" 04/07/05 2:09 PM >>> For all this is worth, here is my experience with plus slides. I have found no differences in tissue attachment in the following brands of plus slides - VWR, Fisher, Stat Labs superfrost plus and histobond slides - I would use any one of these for immuno's without fear of losing my sections. I have tried Surgipaths snowcoat x-tra and some plus slide that Mercedes Medical sells (made in China). In my experience the immuno's I did with the slides from surgipath and Mercedes medical, the tissue sections fell of the slides (with both automated and very careful handling of manual methods) I would never use those slides for immunos or even fixed frozen sections. I cut some fixed tissue for frozen and used surgipaths snowcoat extra and the sections fell off, but adhered just fine to the the StatLab histobond slides. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From leahcox27 <@t> yahoo.com Fri Apr 8 07:28:11 2005 From: leahcox27 <@t> yahoo.com (Leah Cox) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] slides for Ventana In-Reply-To: 6667 Message-ID: <20050408122811.65077.qmail@web50204.mail.yahoo.com> Esco superfrost slides work as well. On our Ventana, the tissue doesn't hold the stain with other slides, but no problems at all with the Esco slides. "Donna M. Nolan" wrote:We have been using the colorfrost slides for a year but have been having trouble with the sections lifting on the Ventana Benchmark. I thought it might be the way we pick the section up from the water bath but we tried the SurgiPath slides the other day and those sections stayed on just fine. The SurgiPath slides we purchased seem to hold the specimen better.. We will have to evaluate the staining. We have tried both slides for H&E staining when the tissue is hard and the SurgiPath usually is the one the tissue stays on. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From portera203 <@t> yahoo.com Fri Apr 8 07:29:19 2005 From: portera203 <@t> yahoo.com (Amy Porter) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Cryostat disposable blades In-Reply-To: 6667 Message-ID: <20050408122919.48633.qmail@web40911.mail.yahoo.com> We use Dura-Edge triple bevel from Source Medical or Dynamic Diagnostics. Very comparable to AccuEdge and cost effective. "Hallada, Teri" wrote:I have finally convinced our pathologists to try disposable blades in the cryostat. I would like to hear from the group about any favorite blades or any other helpful hints. Teri Hallada BS MT CT (ASCP) thallada@noch.org Confidentiality Statement: This message is intended only for the individual or entity to which it is addressed. It may contain privileged, confidential information which is exempt from disclosure under applicable laws. If you are not the intended recipient, please note that you are strictly prohibited from disseminating or distributing this information (other than to the intended recipient) or copying this information. If you have received this communication in error, please notify us immediately by e-mail or by telephone at (616) 842-3600. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Amy S.Porter, HT(ASCP), QIHC Michigan State University - Department of Physiology Division of Human Pathology Investigative Histopathology Laboratory 2201 Biomedical Physical Sciences Room 2133 East Lansing, MI 48824-3320 Ph: 517-355-6475 ext 1480/1481 Fax: 517-432-1368 portera@msu.edu --------------------------------- Do you Yahoo!? Better first dates. More second dates. Yahoo! Personals From Kemlo.Rogerson <@t> elht.nhs.uk Fri Apr 8 07:48:45 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] SurgiPath Macropath[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F174@bhrv-nt-11.bhrv.nwest.nhs.uk> We are having resolution problems with our SurgiPath Macropath; other than replacing the video camera with a high resolution digital one, does anyone have experience with this equipment? Especially, but not exclusively in the UK. Kemlo Rogerson East Lancs Cell Path Manager Burnley 01282 474306 Mobile 07749754194 "Fall seven times. Stand up eight" From Charlene.Henry <@t> STJUDE.ORG Fri Apr 8 08:37:59 2005 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Tissue Micro Arrayer Message-ID: <5CB39BCA5724F349BCB748675C6CA1A202C756BC@SJMEMXMB02.stjude.sjcrh.local> Hi Folks, Does anyone know of another vendor that makes the tissue micro arrayer other than Beecher Instrument. I placed an order with Beecher Instrument in November, 2004 and they are telling us that it will still be 2-3 months before it will be shipped. At this point I'm willing to look for another vendor. Thanks, Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From Charlene.Henry <@t> STJUDE.ORG Fri Apr 8 08:46:03 2005 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] slides for Ventana Message-ID: <5CB39BCA5724F349BCB748675C6CA1A202C756BD@SJMEMXMB02.stjude.sjcrh.local> We also have had trouble with the tissue lifting off of the super frost slides on the Benchmark XT. We use the poly l lysine slides from NewComers and they work great on the Benchmark. I have not lost a single tissue since we started using them. The only problem we had was due to us storing our controls at 4?C and sometimes there was no staining on some of our controls. We now dry all of our slides for 15 minutes before they go on the Benchmark and all work great. We came to the conclusion that the moisture on the slides being pulled from the refrigerator was interfering with the staining process on the Benchmark. Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna M. Nolan Sent: Friday, April 08, 2005 5:13 AM To: Histonet Subject: [Histonet] slides for Ventana We have been using the colorfrost slides for a year but have been having trouble with the sections lifting on the Ventana Benchmark. I thought it might be the way we pick the section up from the water bath but we tried the SurgiPath slides the other day and those sections stayed on just fine. The SurgiPath slides we purchased seem to hold the specimen better.. We will have to evaluate the staining. We have tried both slides for H&E staining when the tissue is hard and the SurgiPath usually is the one the tissue stays on. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From timothy.macatee <@t> med.nyu.edu Fri Apr 8 09:25:54 2005 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Cryostat disposable blades In-Reply-To: <20050407201850.70862.qmail@web30403.mail.mud.yahoo.com> Message-ID: More question about blades. How long does the average blade last? I know it depends on the tissue, etc., but is their an average? Can you tell the difference between a dirty blade and a dull one? How do you clean, or should you, clean your blades? Does wiping off excess paraffin with a kin-wipe dull the blade? These questions come to mind since I'll be cutting all kinds of tissues (core facility) and I want to make sure I do quality sectioning regardless of tissue type or what species it comes from. Another question. What differences of hematoxylin types and or staining times do people use when staining human versus mouse tissues? On 4/7/05 4:18 PM, "Stephen Peters M.D." wrote: > > We use accu edge and have found them to work well. I could not resist > offering this excerpt from my frozen section tutorial. > > > > I find that I always get my best quality section with a new sharp blade. I > think some of > > the places we try to save money in medicine are a bit "pound foolish". Your > > patients surgery is costing thousands of dollars. Hundreds of dollars are > spent > > on disposables including lap pads, gloves, sponges, drapes, cautery, needles, > needle magnets, BP cuff, IV tubing, ..We are conserving pennies on what may > be the > > most important decision impacting on the procedure. Yet some will risk > quality by trying > > to get "20 shaves" out of a disposable blade. > > In my practice I treat every patient to a new section of blade. I will change > it as soon as > > my section quality begins to fall. Some tissues such as tough collagenous > tissues or > > calcified tissues will quickly dull the blade. If I'm having trouble getting a > good section > > with a new blade on occasion I have changed to a second new blade and easily > > prepared a quality section. > > > > Safety Tip If you cut yourself on a blade used on only one patient, you > will > > have minimized your risk of transmittable disease. If you cut yourself on a > blade that > > has been used for days, it is like sleeping with numerous > partners......without the fun! In > > this day and age of doing FNA's with ridiculously flexible long safety needles > and > > using annoying safety scalpels we can justify using a sharp blade for safety > reasons..... > > and get the benefit of awesome frozens every time! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From BDUE <@t> PARTNERS.ORG Fri Apr 8 09:35:30 2005 From: BDUE <@t> PARTNERS.ORG (Due, Brice) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Cryostat disposable blades Message-ID: <59C772E2D8EDF345AE1601F5B60B3CB5027990@PHSXMB7.partners.org> I've had lots of good results with Sturkey's Extremus **High-Profile** blades. I've cut sections ranging in thicknesses from 6um-100um of human muscles (mainly), whole mouse eyes, human lens, and whole mouse pups bones and all, brain with dura. These blades have a super-hard coating which seems to prevent them from dulling or getting scored as easily as Feather/Accu-Edge. They are the thickest blades I've ever seen, and are thus very very stiff. **Note I use their High Profile blades only -- Sturkey has had manufacturing and perhaps design problems in the past with their LOW profile Extremus blades. Their High Profile blades have always been outstanding around here. (But IMHO, for paraffin nothing is as good as a fresh low profile Feather/Accu-Edge blade.) -brice Neuropathology Lab Brigham & Women's Hospital Boston -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Amy Porter Sent: Friday, April 08, 2005 8:29 AM To: Hallada, Teri; Histonet Subject: Re: [Histonet] Cryostat disposable blades We use Dura-Edge triple bevel from Source Medical or Dynamic Diagnostics. Very comparable to AccuEdge and cost effective. "Hallada, Teri" wrote:I have finally convinced our pathologists to try disposable blades in the cryostat. I would like to hear from the group about any favorite blades or any other helpful hints. Teri Hallada BS MT CT (ASCP) thallada@noch.org Confidentiality Statement: This message is intended only for the individual or entity to which it is addressed. It may contain privileged, confidential information which is exempt from disclosure under applicable laws. If you are not the intended recipient, please note that you are strictly prohibited from disseminating or distributing this information (other than to the intended recipient) or copying this information. If you have received this communication in error, please notify us immediately by e-mail or by telephone at (616) 842-3600. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Amy S.Porter, HT(ASCP), QIHC Michigan State University - Department of Physiology Division of Human Pathology Investigative Histopathology Laboratory 2201 Biomedical Physical Sciences Room 2133 East Lansing, MI 48824-3320 Ph: 517-355-6475 ext 1480/1481 Fax: 517-432-1368 portera@msu.edu --------------------------------- Do you Yahoo!? Better first dates. More second dates. Yahoo! Personals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PatPatterson <@t> mhd.com Fri Apr 8 09:52:51 2005 From: PatPatterson <@t> mhd.com (Patterson, Pat) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Tape Coverslip and undecalcified bone Message-ID: <293C7C19EFF7D611AE1A0002A53F81140FA634AF@omega.mhd.com> Hi to all- I wanted to know whether anyone out there who uses the TissueTek tape coverslipper - has had a problem with bone sections having a brown precipitate when they are not fully decalcified? Was you only solution to hand coverslip them? (or obviously make sure sections are fully decalcified). Thanks for your help - Pat Patterson *********************************************************************** This electronic transmission contains information from Methodist Health System and should be considered confidential and privileged. The information contained in the above messages is intended only for the use of the individual(s) and entity(ies) named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution, or use of this information is prohibited. If you receive this transmission in error, please notify the sender immediately by return e-mail. Methodist Health System, its subsidiaries and affiliates hereby claim all applicable privileges related to the transmission of this communication. From JWEEMS <@t> sjha.org Fri Apr 8 09:57:02 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Charging for decal Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA458FB@sjhaexc02.sjha.org> Do you folks bill decal per block or per specimen? Thanks Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. -------------- next part -------------- Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From krat18 <@t> aol.com Fri Apr 8 11:49:25 2005 From: krat18 <@t> aol.com (krat18@aol.com) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Cryostat disposable blades In-Reply-To: Message-ID: <8C70A720EB0A7CC-EDC-31BA@mblk-d28.sysops.aol.com> We have had very good results with Thermo Shandon Teflon-coated high profile blades. I have cut a whole tray (24 blocks) frequently with one blade if there's no calcium (not to mention staples!) in the tissue. You should NOT wipe off Teflon-coated blades, since it removes the Teflon --- we usually brush paraffin trimmings away. Karen krat18@aol.com From krat18 <@t> aol.com Fri Apr 8 11:55:02 2005 From: krat18 <@t> aol.com (krat18@aol.com) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Charging for decal In-Reply-To: <83AACDB0810528418AA106F9AE9B7F7EA458FB@sjhaexc02.sjha.org> References: <83AACDB0810528418AA106F9AE9B7F7EA458FB@sjhaexc02.sjha.org> Message-ID: <8C70A72D79ECE34-EDC-329D@mblk-d28.sysops.aol.com> We bill per specimen. Karen krat18@aol.com From TillRenee <@t> uams.edu Fri Apr 8 12:41:29 2005 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] flourescent Message-ID: Is there a basic protocol for doing fluorescent IHC on frozen or paraffin sections? Is it better to use a primary antibody conjugated with FITC or whatever, instead of with the secondary? I never thought it would be such a headache figuring out fluorescence staining on sectioned tissues as opposed to cells. Also, do you need to get all the reagent separate, or can some of the detections kits be used for fluorescence? Thanks. Renee' Till, HT Arkansas Childrens Nutrition Center 1120 Marshall Little Rock, AR 72202 (501) 364-2774 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From dpahisto <@t> yahoo.com Fri Apr 8 13:00:33 2005 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Mohs Training Message-ID: <20050408180033.65751.qmail@web32106.mail.mud.yahoo.com> Does anyone know of a training program, or classe/symposiums (in California) for learning Moh's techniques? Cindy DuBois, HT ASCP Delta Pathology Assoc. Stockton, CA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From tissuearray <@t> hotmail.com Fri Apr 8 13:38:27 2005 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Tissue Micro Arrayer In-Reply-To: <5CB39BCA5724F349BCB748675C6CA1A202C756BC@SJMEMXMB02.stjude.sjcrh.local> Message-ID: Charlene, Chemicon makes a Tissue Microarray but I would not recommend it. I tested it and found some serious problems with it. If you want to read my write-up go to my website. [1]www.arrayworkshop.com I also have an arrayer that I am having designed and it is being developed at this time. It will hopefully be on the market within the next 3 to 6 months. It is a while to wait but, I can give the company selling it your email? And when it comes on the market you will be notified to check it out. Sorry I can't be of more help. Thom Jensen >From: "Henry, Charlene" >To: >Subject: [Histonet] Tissue Micro Arrayer >Date: Fri, 8 Apr 2005 08:37:59 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by MC6-F29.hotmail.com with Microsoft SMTPSVC(6.0.3790.211); Fri, 8 Apr 2005 06:42:09 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by swlx162.swmed.edu with esmtp (Exim 4.34)id 1DJtgl-0004C5-7U; Fri, 08 Apr 2005 08:38:28 -0500 >Received: from [199.165.152.166] (helo=swlx166.swmed.edu)by swlx162.swmed.edu with esmtp (Exim 4.34) id 1DJtgP-0004By-TEfor histonet@lists.utsouthwestern.edu; Fri, 08 Apr 2005 08:38:12 -0500 >Received: from [192.55.208.20] (helo=mgate1.stjude.org)by swlx166.swmed.edu with esmtp (Exim 4.44) id 1DJtgO-0004aI-DYfor histonet@lists.utsouthwestern.edu; Fri, 08 Apr 2005 08:38:01 -0500 >X-Message-Info: K7FBVhI5yFOWY9O2thoWbPd+f/K9bp6jb8wWQSmaOWs= >X-SEF-Processed: 5_0_0_713__2005_04_08_08_37_59 >X-SEF-FD9E3BCC-24E9-4B6F-96E-FFC4B78-SJCRH: 1 >X-MimeOLE: Produced By Microsoft Exchange V6.5.7226.0 >Content-class: urn:content-classes:message >X-MS-Has-Attach: >X-MS-TNEF-Correlator: >Thread-Topic: Tissue Micro Arrayer >Thread-Index: AcU8QC2uAvPH/iFVQv+1I3GN9ATyJg== >X-OriginalArrivalTime: 08 Apr 2005 13:37:59.0596 (UTC)FILETIME=[2DEC46C0:01C53C40] >X-Scan-Signature: 565204f95959f4d9279b065632258925 >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology andrelated fields >List-Unsubscribe: , >List-Archive: >List-Post: >List-Help: >List-Subscribe: , >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 7b5a3038135de56cb8cb078a17a45140 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu > >Hi Folks, >Does anyone know of another vendor that makes the tissue micro arrayer >other than Beecher Instrument. I placed an order with Beecher Instrument >in November, 2004 and they are telling us that it will still be 2-3 >months before it will be shipped. At this point I'm willing to look for >another vendor. >Thanks, > >Charlene Henry HT (ASCP), QIHC >Histology/Immunohistochemistry Section Head >Department of Pathology >St. Jude Children's Research Hospital >901-495-3191 >fax 901-495-3100 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. http://www.arrayworkshop.com/ From Karen_Skish <@t> rush.edu Fri Apr 8 13:59:36 2005 From: Karen_Skish <@t> rush.edu (Karen_Skish@rush.edu) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Great opportunity in Chicago Message-ID: Looking for experienced histology technician, full-time. Responsibilities include processing, embedding, cutting, and staining of brain tissue from multiple research studies on aging and Alzheimer?s disease. Willingness to perform brain, spinal cord, nerve, and muscle removal at autopsy a plus. Great work environment. If interested, send letter/resume to Karen_skish@rush.edu, or apply on-line at www.jobsatrush.com. Refer to Req#EP17965 Research Assistant 2?Brain Bank. Karen M Skish, MS, PA(ASCP)MT Rush University Medical Center Rush Alzheimer's Disease Center Laboratory Cohn Research Building, Lab 436 1735 West Harrison Street Chicago IL 60612 From DRG <@t> Stowers-Institute.org Fri Apr 8 14:17:42 2005 From: DRG <@t> Stowers-Institute.org (Grant, Debra) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Mouse femurs and tibias fixation time Message-ID: Hi all, I am processing some mouse femurs and tibias for IHC. Any recommendations as to amount of fixation time for Zinc formalin before paraffin processing? Thanks in advance! Cheers! Debby Grant Histology Specialist I Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org From GODSGIRLNOW <@t> msn.com Fri Apr 8 14:35:28 2005 From: GODSGIRLNOW <@t> msn.com (Roxanne Soto) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Charging for decal References: <83AACDB0810528418AA106F9AE9B7F7EA458FB@sjhaexc02.sjha.org> Message-ID: We charge per specimen. Roxanne ----- Original Message ----- From: Weems, Joyce To: Histonet Sent: Friday, April 08, 2005 10:57 AM Subject: [Histonet] Charging for decal Do you folks bill decal per block or per specimen? Thanks Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From Jackie.O'Connor <@t> abbott.com Fri Apr 8 14:40:32 2005 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Mouse femurs and tibias fixation time Message-ID: I let them fix for IHC 24-48 hours. Are you going to decalcify? I decalcify in a formic acid decal, then trim away some of the cortical bone for the perspective I want (I'm doing whole legs). Since they bow to the right or left, I can get the surface I want. Trimming away the cortical bone enables the processing solutions to penetrate much better, and I have no artifacts. Jacqueline M. O'Connor HT(ASCP) Assistant Scientist GPRD Cancer Research Abbott Laboratories, Abbott Park, IL Jackie.OConnor@abbott.com "Grant, Debra" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/08/2005 02:17 PM To: "Histonet" cc: Subject: [Histonet] Mouse femurs and tibias fixation time Hi all, I am processing some mouse femurs and tibias for IHC. Any recommendations as to amount of fixation time for Zinc formalin before paraffin processing? Thanks in advance! Cheers! Debby Grant Histology Specialist I Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lindas <@t> awesomenet.net Fri Apr 8 14:48:19 2005 From: lindas <@t> awesomenet.net (Linda Davis) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Charging for decal References: <83AACDB0810528418AA106F9AE9B7F7EA458FB@sjhaexc02.sjha.org> Message-ID: <006901c53c73$ea721da0$860ac942@D6JLZ851> We bill per specimen. Linda Davis ----- Original Message ----- From: "Weems, Joyce" To: "Histonet" Sent: Friday, April 08, 2005 9:57 AM Subject: [Histonet] Charging for decal > Do you folks bill decal per block or per specimen? > > Thanks > Joyce > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of > this information is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to the > message and deleting it from your computer. > Thank you. Saint Josep From mcauliff <@t> umdnj.edu Fri Apr 8 18:02:16 2005 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] flourescent In-Reply-To: References: Message-ID: <42570D78.2080604@umdnj.edu> Hi Renee: The basic princilpes and protocols are pretty much the same for all immunos, if you can do cells sections should not be that much of a problem. Easy for me to say! Get a DAKO kit, a Vector kit, a Zymed kit or whatever and follow the instructions. Call the maker of the kit before you order to be sure you are getting the correct kit for your application. If possible, go to a lab that does these regularly and learn from someone with experience. Go to the DAKO USA site and download their excellent immunostaining manual. It will teach you everything you need and more. Geoff Till, Renee wrote: >Is there a basic protocol for doing fluorescent IHC on frozen or >paraffin sections? Is it better to use a primary antibody conjugated >with FITC or whatever, instead of with the secondary? I never thought it >would be such a headache figuring out fluorescence staining on sectioned >tissues as opposed to cells. Also, do you need to get all the reagent >separate, or can some of the detections kits be used for fluorescence? > >Thanks. > > > >Renee' Till, HT > > > >Arkansas Childrens Nutrition Center > >1120 Marshall > >Little Rock, AR > >72202 > > > >(501) 364-2774 > > > > >Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Vickroy.Jim <@t> mhsil.com Fri Apr 8 15:07:01 2005 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] microtome question Message-ID: Has anybody experienced any problems with the block holders on the Shandon Finesse microtomes? We like the instruments but we are having some problems with the block holders not holding the tissue firm enough and therefore the blocks can move and therefore chunk. We have tried a couple of new holders but are experiencing the same problem. Any ideas? Jim Vickroy Memorial Medical Center Surgical Pathology Dept. Springfield, Illinois From liz <@t> premierlab.com Fri Apr 8 15:21:22 2005 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Mouse femurs and tibias fixation time Message-ID: <000201c53c78$887c3270$76d48a80@AMY> Debra I agree with Jackie we fix for 24-48 hours and then decal in 5% formic acid. We section the knees in a frontal plane, so we remove most of the muscle prior to placement in decal. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grant, Debra Sent: Friday, April 08, 2005 12:18 PM To: Histonet Subject: [Histonet] Mouse femurs and tibias fixation time Hi all, I am processing some mouse femurs and tibias for IHC. Any recommendations as to amount of fixation time for Zinc formalin before paraffin processing? Thanks in advance! Cheers! Debby Grant Histology Specialist I Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RCHIOVETTI <@t> aol.com Fri Apr 8 15:25:21 2005 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] microtome question Message-ID: In a message dated 4/8/2005 1:07:44 PM US Mountain Standard Time, Vickroy.Jim@mhsil.com writes: > we are having > some problems with the block holders not holding the tissue firm enough > and therefore the blocks can move and therefore chunk. We have tried a > couple of new holders but are experiencing the same problem. Jim, You may already have ruled this out, but I wonder if the problem could be in the knife holder instead of the cassette clamp? It's strange that you get the same problem with more than one new specimen holder...nless there was some kind of manufacturing defect that went undetected, I guess. My Two Cents' Worth! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Independent Consultant for The Science, Technology and Industrial Sectors 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Member, Arizona Small Business Association - ASBA From ohenry <@t> dfw.net Fri Apr 8 15:58:48 2005 From: ohenry <@t> dfw.net (Susan Owens) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] re:Tape Coverslip and undecalcified bone Message-ID: <005701c53c7d$c3fc5640$63dd3040@Nationwide.net> Pat, we have been having the same problem for the last 12 months or so and it only effects bone/cartilage. We do know the following. 1. More of a problem for us with knee cartilage. 2. But not all knee cartilage specimens. 3. It's NOT a precipitate. It you take one straight from the coverslipper to the scope you can actually see it happening. I looks like the tape is actually lifting up and the xylene removed from certain pieces of tissue. You can actually see it move across your field from normal looking tissue to what you call 'brown precipitate'.. 4. It also appears to happen more on the slightly thicker sections. Not thick, just thicker then usual. 5. We believe it to be a tape problem. 6. Only hand coverslips corrects the problem. We did experience something like this several years ago and after a while it stop/corrected. Right now this is not a big problem for us since it only effects 2-3 slides a day. Like I said, for us it's only effecting the non-decaled knee cartilage. I mean to call the company, but just haven't done so. Susan Owens Susan Owens-Texas ohenry@dfw.net voice: 817-261-7938 fax: 817-548-9876 "A bad day at the dog show is better then a good day at work!" >Message: 1 >Date: Fri, 8 Apr 2005 09:52:51 -0500 >From: "Patterson, Pat" >Subject: [Histonet] Tape Coverslip and undecalcified bone >To: "'histonet@lists.utsouthwestern.edu'" >Hi to all- >I wanted to know whether anyone out there who uses the TissueTek tape >coverslipper - has had a problem with bone sections having a brown >precipitate when they are not fully decalcified? Was you only solution to >hand coverslip them? (or obviously make sure sections are fully >decalcified). >Thanks for your help - >Pat Patterson From sakima <@t> bigpond.net.au Fri Apr 8 18:57:08 2005 From: sakima <@t> bigpond.net.au (Satoshi Akima) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] flourescent In-Reply-To: References: Message-ID: Hi Renee, I do confocal microscopy rather than on paraffin sections but I can tell you this: Try to get a primary conjugated antibody where possible. The more steps you have, the more steps that can go wrong. It's also less expensive to get a primary conjugated antibody. Many antibodies that are made for FACS can also be used for immunofluorescent or immunohistochemistry. Toshi Akima PhD Student Centre for Transplantation and Renal Research Westmead Millenium Institute Sydney, Australia On 09/04/2005, at 3:41 AM, Till, Renee wrote: > Is there a basic protocol for doing fluorescent IHC on frozen or > paraffin sections? Is it better to use a primary antibody conjugated > with FITC or whatever, instead of with the secondary? I never thought > it > would be such a headache figuring out fluorescence staining on > sectioned > tissues as opposed to cells. Also, do you need to get all the reagent > separate, or can some of the detections kits be used for fluorescence? > > Thanks. > > > > Renee' Till, HT > > > > Arkansas Childrens Nutrition Center > > 1120 Marshall > > Little Rock, AR > > 72202 > > > > (501) 364-2774 > > > > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From yelkaco <@t> ticon.net Fri Apr 8 20:28:03 2005 From: yelkaco <@t> ticon.net (Steven Coakley) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] question Message-ID: <001801c53ca3$71a06500$8284b542@oemcomputer> Does anyone have information as to how the Microm STP 120 tissue processor does on processing tissue. It has a "shake: and "spin" feature but no vacuum or heated reagents like the VIP. Steven C From yelkaco <@t> ticon.net Fri Apr 8 21:01:00 2005 From: yelkaco <@t> ticon.net (Steven Coakley) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Microm STP 120 Message-ID: <00b101c53ca8$0ab16f60$8284b542@oemcomputer> Our lab is looking at the Microm STP 120 tissue processor. It does not have vacuum or heated reagents but does feature "shake" and "spin"?? Has anyone used this processor? Any opinions? Steven C From sakima <@t> bigpond.net.au Sat Apr 9 07:26:59 2005 From: sakima <@t> bigpond.net.au (Satoshi Akima) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Is TUNEL as waste of time? Message-ID: <4ccebfb3ec1afff4fd701714e8afc816@bigpond.net.au> Dear all, I am looking into the Roche TUNEL kit and have been getting various opinions. Some say it is easy and reviewers will ask for a TUNEL stain in preference to other methods such as staining for caspase-3. However many people find that they get non-specific staining and even where things seem to have gone well you get a great many cells that seem absolutely healthy staining up positive. Do people think that TUNEL is a sham technique that ivory tower reviewers who never get their hands dirty in the lab ask for, never realising how hopeless the technique is? Or is it still a genuinely useful tool? Toshi Akima PhD Student Centre for Transplantation and Renal Research Westmead Millenium Institute Sydney, Australia From cmeiers <@t> thelabrat.com Sat Apr 9 09:16:37 2005 From: cmeiers <@t> thelabrat.com (Christophe Meiers) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] great opportunity Message-ID: <20050409141637.C092C37D00@sitemail.everyone.net> fantastic opportunity for HistoTech Supervisor, Biotech company Lexicon Genetics, The Woodlands, Texas _________________________________________________________________ Search the web here using Google-http://www.thelabrat.com/search.html From gu.lang <@t> gmx.at Sat Apr 9 10:14:35 2005 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] polymer-detection Message-ID: Hi histonetters To those, who use the Ventana Benchmark XT. If you substitute the biotin-avidin-detection with polymer-detection, will the protocols be noticeable shorter? And is the quality of staining better or worse or equal? Are there any limitations for the substitution (routine diagnostic histology)? Thank you for any hints. Gudrun Lang Linz, Austria From sfarley <@t> seattlecca.org Sun Apr 10 15:22:43 2005 From: sfarley <@t> seattlecca.org (Farley, Sunni R) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Taft's Method For Nucleic Acids Message-ID: <5E6BFDF4F0AB2C4DA69CF4473FC7B948323307@wala01.seattlecca.org> I am looking for information regarding Methyl Green-Pyronin staining for nucleic acids. I have contacted Rowley Biochemical Inc (Danvers, MA) about their Methyl Green Pyronin stain and Differentiating Solution. They provided me with the chemical composition of these solutions and also the protocol for Taft's Method for Nucleic Acids. Does anybody routinely use these items and this method? Also, have you used them on B5-fixed tissue? Any information and/or suggestions would be much appreciated. Thank you for your time! Sunni R. Farley Histotechnician Pathology Department Seattle Cancer Care Alliance 825 Eastlake Ave East Seattle, Washington 98109 (206) 288-1312 This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From onep00 <@t> hotmail.com Sun Apr 10 22:51:48 2005 From: onep00 <@t> hotmail.com (Pam O'Neill) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] tissue softening agent Message-ID: Anyone with experience in paleo pathology----- I am looking for information on a softening agent from England called "comfort". It is used in the textile industry, but supposedly is good for softening dessicated tissue. A company name and a phone number would be very helpful. Thankyou, Pam O'Neill SVMMC Toledo, Ohio _________________________________________________________________ Don’t just search. Find. Check out the new MSN Search! http://search.msn.click-url.com/go/onm00200636ave/direct/01/ From jkiernan <@t> uwo.ca Mon Apr 11 00:31:48 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Is TUNEL as waste of time? References: <4ccebfb3ec1afff4fd701714e8afc816@bigpond.net.au> Message-ID: <425A0BC4.7277A907@uwo.ca> TUNEL and the related technique of in situ nick translation are in situ end-labelling (ISEL) methods. They work by adding labelled nucleotides to the fractures in broken DNA molecules. Many cells peg out quietly (apoptosis), either because they are no longer needed (normal) or because of disease. In a cell that's about to die, enzymes become active that cut DNA into short fragments. The cut ends provide binding sites for the labelled nucleotides used in ISEL methods. ---- Satoshi Akima wrote: > > Dear all, > > I am looking into the Roche TUNEL kit and have been getting various > opinions. Some say it is easy and reviewers will ask for a TUNEL stain > in preference to other methods such as staining for caspase-3. However > many people find that they get non-specific staining and even where > things seem to have gone well you get a great many cells that seem > absolutely healthy staining up positive. > > Do people think that TUNEL is a sham technique that ivory tower > reviewers who never get their hands dirty in the lab ask for, never > realising how hopeless the technique is? Or is it still a genuinely > useful tool? > > Toshi Akima > PhD Student > Centre for Transplantation and Renal Research > Westmead Millenium Institute > Sydney, Australia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Mon Apr 11 00:42:49 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Taft's Method For Nucleic Acids References: <5E6BFDF4F0AB2C4DA69CF4473FC7B948323307@wala01.seattlecca.org> Message-ID: <425A0E59.F30D7D08@uwo.ca> You enquired about "Taft's method" for green DNA and red RNA. Taft studied and possibly improved the method. Brachet had recognized that it stained the nucleic acids in different colours, about 10 years earlier. The original technique dated from about 1910 (Unna & Pappenheim); it helped to define the plasma cell. If you mean the technique described and investigated by E.B.Taft in 1951 (Stain Technol 26,205; Exp Cell Res 2,312), it is a complicated procedure, developed at a time when methyl green was always contaminated with crystal violet and pyronine dyes were frequently unreliable. Things are easier these days. ALSO: Please note that the correct spelling is pyronine, not pyronin. Organic bases have -ine endings. Methyl green (CI 42585) has probably not been manufactured for 30 or 40 years. Dye powders and solutions are still labelled "methyl green" even by the most trustworthy suppliers, but the dye they contain is ethyl green (CI 42590). The catalogue of at least one huge company indexes ethyl and methyl green, both as CI 42590. That's almost honest. This may seem a trivial point, but it is not. Methyl green spontaneously loses a methyl group from its quaternary nitrogen atom. The product is crystal violet, which must be removed from the aqueous dye solution by repeated extractions with chloroform in a separating funnel. (I did this in the 1960s and early 1970s, and it could take up half a working day.) Ethyl green is much more stable than methyl green, and the chloroform extraction is not needed. The staining method should be renamed ethyl green-pyronine Suppliers should proudly sell ethyl green rather than the old, troublesome methyl green. The Biological Stain Commission certifies ethyl green, pyronine B and pyronine Y. The tests and requirements have been published by Penney et al (2002) in Biotech Histochem 77: 237-275. Staining with ethyl green & pyronine always need careful experimentation to get critical separation of the DNA and RNA colours. John Kiernan London, Canada. -------------------------------------- "Farley, Sunni R" wrote: > I am looking for information regarding Methyl Green-Pyronin staining for > nucleic acids. I have contacted Rowley Biochemical Inc (Danvers, MA) about > their Methyl Green Pyronin stain and Differentiating Solution. They > provided me with the chemical composition of these solutions and also the > protocol > for Taft's Method for Nucleic Acids. Does anybody routinely use these > items and this method? Also, have you used them on B5-fixed tissue? > Any information and/or suggestions would be much appreciated. Thank you for > your time! > > Sunni R. Farley > Histotechnician > Pathology Department > Seattle Cancer Care Alliance > 825 Eastlake Ave East > Seattle, Washington 98109 > (206) 288-1312 > From kelly.mcqueeney <@t> bms.com Mon Apr 11 08:11:42 2005 From: kelly.mcqueeney <@t> bms.com (Kelly D Mcqueeney) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Is TUNEL as waste of time? In-Reply-To: <4ccebfb3ec1afff4fd701714e8afc816@bigpond.net.au> References: <4ccebfb3ec1afff4fd701714e8afc816@bigpond.net.au> Message-ID: <425A778E.6090901@bms.com> What about apoptotic CK-18 antibody? It is supposed to be more sensitive than TUNEL. You can use it with Ki-67 antobody. Good luck, Kelly Satoshi Akima wrote: > Dear all, > > I am looking into the Roche TUNEL kit and have been getting various > opinions. Some say it is easy and reviewers will ask for a TUNEL stain > in preference to other methods such as staining for caspase-3. However > many people find that they get non-specific staining and even where > things seem to have gone well you get a great many cells that seem > absolutely healthy staining up positive. > > Do people think that TUNEL is a sham technique that ivory tower > reviewers who never get their hands dirty in the lab ask for, never > realising how hopeless the technique is? Or is it still a genuinely > useful tool? > > Toshi Akima > PhD Student > Centre for Transplantation and Renal Research > Westmead Millenium Institute > Sydney, Australia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adelsyscarol <@t> yahoo.com Mon Apr 11 09:07:00 2005 From: adelsyscarol <@t> yahoo.com (Carol Wilson) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] looking for manual for Damon/IEC DPR 6000 Centrifuge Message-ID: <20050411140701.86955.qmail@web51506.mail.yahoo.com> I know this is a longshot, but does anyone out there in histoland have a manual for a Damon/IEC DPR 6000 centrifuge? I'd be willing to pay shipping fee and copying costs, or reasonable price. Thanks Carol Wilson, HT(ASCP) Histology and Laboratory Products and Service Adelsys,Inc. 1925 Lee Rd. Cleveland Heights, OH 44118 216-932-7500 Fax 216-932-7850 --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. From John.Sheppard <@t> Health-Partners.org Mon Apr 11 09:39:34 2005 From: John.Sheppard <@t> Health-Partners.org (John.Sheppard@Health-Partners.org) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] RE:microtome question Message-ID: We have been using the Finesse Microtomes for over six years. We have not had any problems with the block holder. What thickness are you trimming into the tissue at? I usually trim paraffin blocks at 25 microns. You can chip tissue out if you are trimming too agressively. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From leswes <@t> shaw.ca Mon Apr 11 10:16:29 2005 From: leswes <@t> shaw.ca (Lesley Weston) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] tissue softening agent In-Reply-To: Message-ID: "Downey" or any other liquid laundry softener will do just as well - that's what "Comfort" is. Lesley Weston. -- > From: Pam O'Neill > Date: Mon, 11 Apr 2005 03:51:48 +0000 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] tissue softening agent > > Anyone with experience in paleo pathology----- I am looking for information > on a softening agent from England called "comfort". It is used in the > textile industry, but supposedly is good for softening dessicated tissue. A > company name and a phone number would be very helpful. Thankyou, Pam O'Neill > SVMMC Toledo, Ohio > > _________________________________________________________________ > Don?t just search. Find. Check out the new MSN Search! > http://search.msn.click-url.com/go/onm00200636ave/direct/01/ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Apr 11 10:39:36 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Is TUNEL as waste of time? In-Reply-To: <425A778E.6090901@bms.com> Message-ID: <200504111540.j3BFeMeb080662@pro12.abac.com> In my experience, with what we were doing Tunnel was not helpful at all. We could not distinguish apoptosis from necrosis. We had a lot of necrosis so this was useless to us. Cleaved Caspase 3 in my hands seemed only to label the cells undergoing apoptosis. CC3 is cheaper and more consistently reliable in my experience. Note: I had been paying over $600. for a very small amount of the CC3 antibody from Cell Signaling but just recently tried the same clone from BioCare and found it to be just as good at about 1/4 the price. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly D Mcqueeney Sent: Monday, April 11, 2005 6:12 AM To: Satoshi Akima Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Is TUNEL as waste of time? What about apoptotic CK-18 antibody? It is supposed to be more sensitive than TUNEL. You can use it with Ki-67 antobody. Good luck, Kelly Satoshi Akima wrote: > Dear all, > > I am looking into the Roche TUNEL kit and have been getting various > opinions. Some say it is easy and reviewers will ask for a TUNEL stain > in preference to other methods such as staining for caspase-3. However > many people find that they get non-specific staining and even where > things seem to have gone well you get a great many cells that seem > absolutely healthy staining up positive. > > Do people think that TUNEL is a sham technique that ivory tower > reviewers who never get their hands dirty in the lab ask for, never > realising how hopeless the technique is? Or is it still a genuinely > useful tool? > > Toshi Akima > PhD Student > Centre for Transplantation and Renal Research > Westmead Millenium Institute > Sydney, Australia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sue.Kapoor <@t> uhsi.org Mon Apr 11 11:07:52 2005 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] need hood for Tissue Tek slide stainer Message-ID: <61E9F2400F53D5119CFC00508B44E33B02E9E0CE@khmcexch.uhsi.org> To all vendors - I'm looking for a used or refurbished hood for my slide stainer, Tissue Tek DRS-601. Please email me at: sue.kapoor@uhsi.org thank you, Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 From malek.j <@t> ghc.org Mon Apr 11 13:09:47 2005 From: malek.j <@t> ghc.org (Malek, Jack M) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Immunohistochemical staining interference by ethanol vs alcohol blend Message-ID: <63EB2FB8C50AD242898B76E2B2C7C12202121AC6@ROC2T9.ghc.org> Has anyone experienced interference with immunohistochemical staining when using an ethanol blend versus an alcohol blend? We are preparing to switch from Richard Allan's Flex 100 (isopropanol/methanol blend) to their Dehydrant (ethanol/isopropanol/methanol blend). Thank you in advance, Jack Malek GHC Pathology Seattle, Washington 206-326-3251 Malek.j@ghc.org From SBarnes <@t> elch.org Mon Apr 11 13:58:49 2005 From: SBarnes <@t> elch.org (Sue Barnes) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] formula 83 Message-ID: Hi, Has anyone had any experience with the formula 83 replacement for xylene? Received a flyer on it and was wondering if anyone was using it yet. Sue Barnes CT,HT (ASCP) sbarnes@elch.org East Liverpool City Hospital East Liverpool, Ohio From malek.j <@t> ghc.org Mon Apr 11 14:16:30 2005 From: malek.j <@t> ghc.org (Malek, Jack M) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Ethanol Interference with Immunohistochemical staining Message-ID: <63EB2FB8C50AD242898B76E2B2C7C12202B12539@ROC2T9.ghc.org> Has anyone experienced interference with immunohistochemical staining when using an ethanol blend versus an alcohol blend? We are preparing to switch from Richard Allan's Flex 100 (isopropanol/methanol blend) to their Dehydrant (ethanol/isopropanol/methanol blend). Thank you in advance, Jack Malek GHC Pathology Seattle, Washington 206-326-3251 Malek.j@ghc.org From int09018 <@t> alphahunt.com Mon Apr 11 15:12:15 2005 From: int09018 <@t> alphahunt.com (HCS) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] looking for suggestions on distiguishing protozoa. Message-ID: <000601c53ed2$c1e23990$6601a8c0@hp> Does someone have a suggestion for a stain that would distinguish bacteria from protozoa? Thanks in advance LeRoy Brown HCS www.histocs.com 360-966-7300 From Luis.Chiriboga <@t> med.nyu.edu Mon Apr 11 09:20:26 2005 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] p16 source Message-ID: Hi everyone Can anyone recommend a source for anti-p16 for use in human frozen tissue.......? TIA `Luis From sakima <@t> bigpond.net.au Mon Apr 11 17:35:27 2005 From: sakima <@t> bigpond.net.au (Satoshi Akima) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] RE: Is TUNEL a waste of time? Message-ID: <0787a008ede14e834647a585c31a69e2@bigpond.net.au> On 11/04/2005, at 7:54 PM, Edwards, R.E. wrote: > We abandoned the TUNEL method, many years ago as we felt it > gave too many false +ves, we used H@E staining until reliable > antibodies came along, e.g., caspase 3............ I have found others who use TUNEL staining express a similar opinion about the false positives. I would be interested to know if others too have found an excessive rate of false positives. Still the TUNEL method seems to be viewed by a great many as some sort of Gold Standard. Many find still swear by it as a very good tool. Regarding the issue of specificity - does caspase-3 also help to distinguish between apoptosis and necrosis I wonder? Toshi Akima PhD Student Centre for Transplantation and Renal Research Westmead Millenium Institute Sydney, Australia Toshi From histojock <@t> hotmail.com Mon Apr 11 17:41:48 2005 From: histojock <@t> hotmail.com (Histo Jock) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Re: Tissue Micro Arrayer Message-ID: Hi Charlene, I assume that you ordered a Beecher MTA-II? If so you should expect a wait. Beecher's orders for that model are running months ahead of production. I understand that it's been a very popular machine. If you ask them nicely they will send you an MTA-I to use until your MTA-II is ready or you can just change the order to an MTA-I. For most labs the MTA-I is more than you need anyway. Beecher normally has long wait times for everything they make. Beecher is the only source for tissue arrayers as they own all of the patents on them. Chemicon makes an arrayer by licensing a design from Beecher but as noted already it is both inferior to and hugely more expensive than the Beecher models ($60K!!! Chemicon vs. $10-20k Beecher). Nobody who really knows tissue arrays buys the Chemicon. I've never heard anybody speak highly of it. HistoJock >Hi Folks, >Does anyone know of another vendor that makes the tissue micro >arrayer other than Beecher Instrument. I placed an order with >Beecher >Instrument in November, 2004 and they are telling us that it will still be >2-3 >months before it will be shipped. At this point I'm willing to look for >another vendor. >Thanks, > >Charlene Henry HT (ASCP), QIHC _________________________________________________________________ Don’t just search. Find. Check out the new MSN Search! http://search.msn.click-url.com/go/onm00200636ave/direct/01/ From yelkaco <@t> ticon.net Mon Apr 11 18:02:12 2005 From: yelkaco <@t> ticon.net (Steven Coakley) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Embedding Center Message-ID: <003901c53eea$8f6bc040$5e81b542@oemcomputer> Our Labs looking for a smaller size used or refurbished embedding center. Thanks From immrstambo <@t> hotmail.com Mon Apr 11 19:29:34 2005 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] formula 83 In-Reply-To: Message-ID: We've been using it now for about a month. We tried it about a year ago and finally got approval to switch. I love it! It is almost as good as xylene. Good Luck! Christine Tambasco, HT (ASCP) ST. Mary's Hospital 427 Guy Park Ave Amsterdam, New York 12010 >From: "Sue Barnes" >To: >Subject: [Histonet] formula 83 >Date: Mon, 11 Apr 2005 14:58:49 -0400 > >Hi, >Has anyone had any experience with the formula 83 replacement for xylene? > >Received a flyer on it and was wondering if anyone was using it yet. > >Sue Barnes CT,HT (ASCP) > >sbarnes@elch.org >East Liverpool City Hospital >East Liverpool, Ohio > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From immrstambo <@t> hotmail.com Mon Apr 11 19:40:02 2005 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] microtome question In-Reply-To: Message-ID: maybe it's the cassettes that are not fitting right? It's possible to get a bad batch. Try it and good luck! Christine Tambasco, HT (ASCP) St. Mary's Hospital @ Amsterdam, New York >From: RCHIOVETTI@aol.com >To: Vickroy.Jim@mhsil.com, histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] microtome question >Date: Fri, 8 Apr 2005 16:25:21 EDT > >In a message dated 4/8/2005 1:07:44 PM US Mountain Standard Time, >Vickroy.Jim@mhsil.com writes: > > > we are having > > some problems with the block holders not holding the tissue firm enough > > and therefore the blocks can move and therefore chunk. We have tried a > > couple of new holders but are experiencing the same problem. > >Jim, > >You may already have ruled this out, but I wonder if the problem could be in >the knife holder instead of the cassette clamp? It's strange that you get the >same problem with more than one new specimen holder...nless there was some >kind of manufacturing defect that went undetected, I guess. > >My Two Cents' Worth! > >Cheers, > >Bob > >Robert (Bob) Chiovetti, Ph.D. >Independent Consultant for The Science, Technology and Industrial Sectors >132 North Elster Drive >Tucson, AZ 85710-3212 USA >Tel./Fax 520-546-4986 >Member, Arizona Small Business Association - ASBA >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From reynaniteraven <@t> yahoo.com Mon Apr 11 20:59:37 2005 From: reynaniteraven <@t> yahoo.com (Jacqueline Lair) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] microtome question Message-ID: <20050412015938.34862.qmail@web81509.mail.yahoo.com> I was having a similar problem with the microtomes in our lab.. the block would chunk out the tissue and the block seemed as if it were barely held into the block holder, very "wobbly like". You could wiggle the block around in the block holder after it was clamped in. This is what I did: Look at the block holder as its held or in the "open" position you would need to have it to insert a block. You should see one or two small openings (the "openings" will either be on the bottom or on the sides of the block holder, depending on which type of microtome you are using.) between the metal on the block holder; i.e. where the metal would come or clamp together to hold a paraffin block. Next, take a pick and scrape the paraffin out of the "opening(s)" in the block holder. When I did the scraping, my block holder had a bunch of paraffin built up "inside" of it, which was causing the block holder to not clamp down as tightly or securely on the block. I find that if I face in many blocks at one time, I have to scrape out the block holder after Ive finished all of my facing. Now, Ive just gotten into the habit of scraping out the block holder after Im done facing to ensure that there is no paraffin build up within the block holder at all. I hope this makes sense? I hope it helps. Jacqueline Lair, HT (ASCP) From kp <@t> vdl.co.nz Tue Apr 12 00:25:56 2005 From: kp <@t> vdl.co.nz (Kerry Patience) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Formula 83 Message-ID: Is formula 83 a xylene substitute? What company produces it and how does the price compare to xylene? From Julie.Burns <@t> nuth.northy.nhs.uk Tue Apr 12 03:29:02 2005 From: Julie.Burns <@t> nuth.northy.nhs.uk (Burns, Julie) Date: Fri Sep 16 15:24:56 2005 Subject: [Histonet] Delaware diamond knives Message-ID: Hi I work in a busy electron microscopy unit and I am considering the purchase of a delaware diamond knive - I was wanting to know of anyones experiences of these diamond knives ? many thanks Julie Burns From jl <@t> eyepath.ku.dk Tue Apr 12 08:13:35 2005 From: jl <@t> eyepath.ku.dk (Jens Lindegaard) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Porcine macrophages Message-ID: Hello Can anyone tell me which antibody to use in order to immunostain porcine macrophages in paraffin embedded sections? Jens Lindegaard M.D. Eye Pathology Institute Frederik V's Vej 11, 1.floor DK-2100 Copenhagen Denmark Phone +45 3532 6083 Fax +45 3532 6080 From Carlos.Prada-Puentes <@t> pat.nhs.uk Tue Apr 12 08:59:39 2005 From: Carlos.Prada-Puentes <@t> pat.nhs.uk (Prada-Puentes Carlos (RW6) PAHNT) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] WT1 Message-ID: Hi there, I have been using WT1 with good results from Santa Cruz biotechnology. My problem is that after 6 months the antibody has become unstable and altough we have concentrated it the nuclear staining is weaker and weaker. The datasheet does not recommend freezing and gives ONLY ONE YEAR!!!! Of guaranteed stability kept at 4 C..Should I freeze it when the new vial comes? Considering we use at a dilution of 1/3000 we need to freeze before we use it all. Thank you to all Dr Carlos Prada-Puentes From sluhisto <@t> yahoo.com Tue Apr 12 09:01:15 2005 From: sluhisto <@t> yahoo.com (Histology SLU) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Known Gastrin Controls Message-ID: <20050412140115.83247.qmail@web51007.mail.yahoo.com> Hello All: Does anyone have any extra human gastrin IHC control blocks that you can spare? Thanks in advance for your help and have a wonderful day! Susan --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From Sue.Kapoor <@t> uhsi.org Tue Apr 12 10:01:14 2005 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] vendors Message-ID: <61E9F2400F53D5119CFC00508B44E33B02E9E0D1@khmcexch.uhsi.org> To all vendors, I'm looking for a used fume hood for a Tissue-Tek DRS-601 slide stainer and a used Tissue-Tek Cryo Console. Please email me at: sue.kapoor@uhsi.org Thank you, Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 From pruegg <@t> ihctech.net Tue Apr 12 10:46:35 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Porcine macrophages In-Reply-To: Message-ID: <200504121546.j3CFkQOD004555@chip.viawest.net> I use RDI-Macropabm-387 (Mac387) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jens Lindegaard Sent: Tuesday, April 12, 2005 6:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Porcine macrophages Hello Can anyone tell me which antibody to use in order to immunostain porcine macrophages in paraffin embedded sections? Jens Lindegaard M.D. Eye Pathology Institute Frederik V's Vej 11, 1.floor DK-2100 Copenhagen Denmark Phone +45 3532 6083 Fax +45 3532 6080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From meyenhmf <@t> umdnj.edu Tue Apr 12 10:49:02 2005 From: meyenhmf <@t> umdnj.edu (meyenhmf@umdnj.edu) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] vendors In-Reply-To: <61E9F2400F53D5119CFC00508B44E33B02E9E0D1@khmcexch.uhsi.org> References: <61E9F2400F53D5119CFC00508B44E33B02E9E0D1@khmcexch.uhsi.org> Message-ID: <1113320942.425bedeee6f1a@webmail.umdnj.edu> I have a slightly used (1 year in a small hospital) ^)! with hood available. Also lots of other EM, Histo and Lab Equipment. Please contact me at: micro@superlink.net Regards, Markus F. Meyenhofer Microscopy Labs micro@superlink.net Quoting "Kapoor, Sue" : > To all vendors, I'm looking for a used fume hood for a Tissue-Tek > DRS-601 > slide stainer and a used Tissue-Tek Cryo Console. > > Please email me at: > sue.kapoor@uhsi.org > > Thank you, > Sue Kapoor, HT (ASCP) > Histology Coordinator > Kenosha Medical Center > Kenosha, WI > 262-653-5570 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- From Joyce.Rush <@t> sjmcmn.org Tue Apr 12 10:55:04 2005 From: Joyce.Rush <@t> sjmcmn.org (Rush, Joyce) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Recycling Message-ID: <2337362E8548BE4C85EE5DD5D526B0851942B4@sjw3smail2.SJMCMN.ORG> We are looking into recycling our alcohol, formalin and Americlear. I'd love to hear any experiences doing this. We are a relatively small user and currently sewer everything. I'm trying to gather information for the day when we can not do this. Thanks, Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 From pruegg <@t> ihctech.net Tue Apr 12 11:11:10 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Tape transfer sections and IHC Message-ID: <200504121611.j3CGB1OD014811@chip.viawest.net> I am doing hrp IHC on tape transfer sections and I am experiencing a problem with unwanted staining on the coating on the polymer coated slides. Does anyone else out there do IHC on the tape sections experience this problem? Please suggest a solution to this. Thank you, Patsy From Connie.Sims <@t> dchstx.org Tue Apr 12 11:18:39 2005 From: Connie.Sims <@t> dchstx.org (Connie Sims) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Recycling Message-ID: <11906C81B7B03E47A6F10CE5F4784449018A9C1F@dchexch01.driscoll.dch> We currently have a CBG recycler. I love it! It does take some tech time and planning but the cost savings and environmental benefits are worth it. Formalin requires rebuffering and alcohol is only 95%. Americlear is completely reuseable. I haven't had any problems so far, its been almost a year now. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rush, Joyce Sent: Tuesday, April 12, 2005 10:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling We are looking into recycling our alcohol, formalin and Americlear. I'd love to hear any experiences doing this. We are a relatively small user and currently sewer everything. I'm trying to gather information for the day when we can not do this. Thanks, Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================================================================== Driscoll Children's Hospital Disclaimer: This e-mail message and all information enclosed, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution of the information enclosed, in its entirety or in part, is strictly prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and immediately destroy all copies of the message. For further assistance or questions please contact the email administrator at Driscoll Children's Hospital at (361) 694-5000. ========================================================================================================= From cfavara <@t> niaid.nih.gov Tue Apr 12 11:52:37 2005 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Porcine macrophages Message-ID: Have you looked into F4/80? c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Tuesday, April 12, 2005 8:47 AM To: 'Jens Lindegaard'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Porcine macrophages I use RDI-Macropabm-387 (Mac387) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jens Lindegaard Sent: Tuesday, April 12, 2005 6:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Porcine macrophages Hello Can anyone tell me which antibody to use in order to immunostain porcine macrophages in paraffin embedded sections? Jens Lindegaard M.D. Eye Pathology Institute Frederik V's Vej 11, 1.floor DK-2100 Copenhagen Denmark Phone +45 3532 6083 Fax +45 3532 6080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Tue Apr 12 12:02:17 2005 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Recycling Message-ID: <3D502BBF5356D31184650090275B750D0346C968@MAIL> We also use a CBG recycler for formalin, xylene, and 95% ethanol. We love it here! It's very easy to use. The unit itself is very reliable. The customer service with CBG is excellent. (my own opinion, no other affiliation with CBG) Stacy McLaughlin -----Original Message----- From: Connie Sims [mailto:Connie.Sims@dchstx.org] Sent: Tuesday, April 12, 2005 11:19 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Recycling We currently have a CBG recycler. I love it! It does take some tech time and planning but the cost savings and environmental benefits are worth it. Formalin requires rebuffering and alcohol is only 95%. Americlear is completely reuseable. I haven't had any problems so far, its been almost a year now. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rush, Joyce Sent: Tuesday, April 12, 2005 10:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling We are looking into recycling our alcohol, formalin and Americlear. I'd love to hear any experiences doing this. We are a relatively small user and currently sewer everything. I'm trying to gather information for the day when we can not do this. Thanks, Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================ ============================ Driscoll Children's Hospital Disclaimer: This e-mail message and all information enclosed, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution of the information enclosed, in its entirety or in part, is strictly prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and immediately destroy all copies of the message. For further assistance or questions please contact the email administrator at Driscoll Children's Hospital at (361) 694-5000. ============================================================================ ============================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If the reader of this e-mail message is not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this e-mail message in error, please immediately notify Cooley Dickinson Healthcare at 413-582-2000 and delete or shred the original message and all copies thereof. Thank you. From sleslie <@t> northernhealth.org Tue Apr 12 12:14:58 2005 From: sleslie <@t> northernhealth.org (Shawn Leslie) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] (no subject) Message-ID: <23A9437CB1BFCA44A975CF3AD9191D1246C41E@NMHEXEVS01.nmh.nmrhs.net> To All, I have a trainee who is about to sit for her HT computer exam, and wondered if anyone has taken it recently, and could offer some helpful hints. Thanks Shawn Leslie Confidentiality Notice: This e-mail message, including any attachments, may contain confidential information. The information is intended only for the use of the individual(s) or entity named above. If you are not the intended recipient, you are notified that any disclosure, copying, distribution, or the taking of any action in reliance on the contents of this e-mail information is prohibited. If you have received this e-mail in error, please contact the sender by reply e-mail and destroy all copies of the original message. From GoodwinD <@t> pahosp.com Tue Apr 12 13:24:11 2005 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] looking for PT work Message-ID: <992899E9EC268548AB8DDE246AF88473055F4FAE@PAHEX01.uphs.upenn.edu> Histo tech looking for PT evening hours in the Philadelphia area. Routine embedding, microtomy, special stains, etc. If interested, please contact Coleen Kiehl at 215-829-6940. From gcallis <@t> montana.edu Tue Apr 12 14:08:01 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Background with tape transfer slides, IHC Message-ID: <6.0.0.22.1.20050412125533.01b14070@gemini.msu.montana.edu> Patsy, I have not had a problem. I do add Tween 20 to all buffers and diluents and we only use 1/2X slides. We find these hold bone perfectly but we do double flash to ensure no lift off. The Tween is 0.05% but you can try and increase concentration higher, 0.2% or so, maybe even higher than that. You may be experiencing ionic interaction of chromogen to the polymer, commonly occurs with plus charge slides also, i.e. ring around the section only you have tinted polymer. I am assuming you are using DAB and that is the background?? I am doing hrp IHC on tape transfer sections and I am experiencing a problem with unwanted staining on the coating on the polymer coated slides. Does anyone else out there do IHC on the tape sections experience this problem? Please suggest a solution to this. Thank you, Patsy Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Tue Apr 12 14:52:24 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Fluorescent immunostaining versus IHC Message-ID: <6.0.0.22.1.20050412130938.01b4a210@gemini.msu.montana.edu> Renee, Our immunofluoroescent staining is commonly done with two fluorophores, and have done it for both confocal and fluorescence microscopy. It isn't anymore difficult but there are some tricks to the trade, so to speak. We basically do it the same as IHC, but have noticed, at least with our antibodies, the primary antibody concentration increases. We cannot use a fluorophore directly conjugated to FITC for our mouse frozen sections and apply these antibodies for fluorescence microscopy - these are murine CD4 FITC or CD8-FITC - we get NO fluorescence. There are reasons for this. With human kidney biopsies and IFA staining, the staining is excellent with directly conjugated primaries - IgG-FITC, etc. although the frozen sections are fixed minimally with cold acetone. However, one can increase the fluorescence signal a great deal ( and you want the brightest signal with the least photobleaching you can get) by using biotinylated primaries then Strepavidin-Alexa dyes OR a purified antibody followed by a fluorophore conjugated secondary i.e. secondary conjugated to FITC or RRX or a rhodamine that is crisp, separates nicely from FITC, and is more resistant to photobleaching. With our murine CD marker work, we prefer using a primary that is purified w/secondary antibody-fluorophore or a biotinylated antibody w/Strepavidin-Alexa dye (488 equivalent to FITC) or Strepavidin-555 (equivalent to Rhodamine). How we combine them for double IFA staining varies. With any fluorophore conjugated secondary, it is a good idea to spin the diluted antibody just before applying to section to eliminate protein aggregates that have fluorophore attached - we refer to this as glowing garbage. One can purchase secondary antibodies from Molecular Probes that are conjugated to Alexa dyes and we only use secondary antibodies adsorbed to the species being stained. You need to be aware of autofluorescence and what causes it - major culprits are NBF or paraformaldehyde fixation - very annoying and Histonet abounds with questions on how to get rid of it, not much fun nor always an easy task. We avoid aldehyde fixatives for this reason and use fresh frozen sections fixed with acetone or acetone/ethyl alcohol mixture. We avoid methanol with CD markers. Some use paraformaldehyde or other fixtives but that must be determined with fixation panels. I have never used a detection kit for IFA work, everything is inhouse dilutions of secondary antibodies or Strepavidin Alexas. We do not use Strepavidin conjugated to any FITC or its derivatives, the rhodamines. We found they did not hold up as well as Alexa conjugates. FITC and FITC derivatives are fluoresceinated versus the Alexa dyes which are sulphonated - with the latter being more stable with less photobleaching when excited. Our technics tend to be rather purist, and we generally leave Tween out of buffers, although some do use it. All incubations with the fluorophore conjugates are done in the dark, so if you use an open immunostainer, it must be covered or do simple manual staining for that portion. We now coverslip using Molecular Probes Prolong Gold antifade, hard set and ready to use. Jackson ImmunoResearch has excellent antibodies - get their hard copy catalog - a wealth of information. Rockland and Southern Biotechnology are two more antibody sources. Molecular Probes has a wonderful handbook although huge, but it is very educational and FREE. Many companies recommend freezing down aliquots of fluorophore conjugates, and their spec sheets tell how to do this. This is a good book both IHC and IFA information: Immunochemistry 2, a practical approach Edited by AP Johnstone and MW Turner, ISBN# 0 19 963609 5, paperback. Purchased from Springer-Verlag publishers, they have a website. very you wrote: Hi Renee, I do confocal microscopy rather than on paraffin sections but I can tell you this: Try to get a primary conjugated antibody where possible. The more steps you have, the more steps that can go wrong. It's also less expensive to get a primary conjugated antibody. Many antibodies that are made for FACS can also be used for immunofluorescent or immunohistochemistry. Toshi Akima On 09/04/2005, at 3:41 AM, Till, Renee wrote: > Is there a basic protocol for doing fluorescent IHC on frozen or > paraffin sections? Is it better to use a primary antibody conjugated > with FITC or whatever, instead of with the secondary? I never thought > it> would be such a headache figuring out fluorescence staining on > sectioned tissues as opposed to cells. Also, do you need to get all the reagent > separate, or can some of the detections kits be used for fluorescence? > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From petepath <@t> yahoo.com Tue Apr 12 14:59:23 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Job opening for experienced Immunohistotechnician in New Jersey Message-ID: <20050412195923.45472.qmail@web30407.mail.mud.yahoo.com> We have a full time opening for an experienced immunohistotech in our clinical histology lab at Hackensack University Medical Center. We use Ventana Benchmark. If interested contact Lorraine Mooney at: lmooney@humed.com From Nancy.Temple <@t> ssfhs.org Tue Apr 12 15:19:10 2005 From: Nancy.Temple <@t> ssfhs.org (Temple Nancy) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Histotech position Message-ID: Position for full time registered histotech, day shift, at St. Francis Hospital, Indianapolis, In. We do routine histology, and are preparing to start automated IHC with Ventana Benchmark. If interested, please contact: Nancy Temple (HT ASCP) Supervisor Histology/Cytology St. Francis Hospital 8111 S. Emerson Ave. Indianapolis, In 46237 317-851-3836 ____________________________________________________________________________ __________________________________ The information contained in this email and any accompanying documents is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited. If you are not the intended recipient(s), please contact the sender by e-mail and destroy all copies of the original message. Thank you. From mrsgbd2001 <@t> yahoo.com Tue Apr 12 15:49:31 2005 From: mrsgbd2001 <@t> yahoo.com (Gareth Davis) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] (no subject) In-Reply-To: 6667 Message-ID: <20050412204931.9311.qmail@web52704.mail.yahoo.com> Hi, I took the HT computer test last December. One helpful hint I can give is to know everything about tissue idenitification, what stain is needed for that tissue, what may go wrong with stain for a specific tissue and how to identify mistakes. I saw so many images, and it really caught me offguard. I expected many, but not as many as I saw and the questions to the images were not what I expected. I did pass with a good score, but I was really surprised that I did. Good luck. Gareth Davis --- Shawn Leslie wrote: > > > To All, > > I have a trainee who is about to sit for her HT > computer exam, and wondered if anyone has taken it > recently, and could offer some helpful hints. Thanks > > Shawn Leslie > > Confidentiality Notice: This e-mail message, > including any attachments, may contain confidential > information. The information is intended only for > the use of the individual(s) or entity named above. > If you are not the intended recipient, you are > notified that any disclosure, copying, distribution, > or the taking of any action in reliance on the > contents of this e-mail information is prohibited. > If you have received this e-mail in error, please > contact the sender by reply e-mail and destroy all > copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From Debbiejsiena <@t> aol.com Tue Apr 12 16:06:33 2005 From: Debbiejsiena <@t> aol.com (Debbiejsiena@aol.com) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Histotech Position Message-ID: <45.260767bf.2f8d9259@aol.com> Full time position registered histotech or histotech that can be registered, day shift at Tissue Techniques, Dallas, Texas. We do routine histology, special stains and IHC with the Ventana Benchmark LT. If interested, please contact: Debbie Siena (HT ASCP, QIHC) - Owner Tissue Techniques Pathology Labs LLC 13016 Bee Street Suite # 100 Dallas, Texas 75234-6158 Office: 972-241-6277 E-mail: tissuetech@juno.com web site: tissuetechpathology.com From lhotaks <@t> mcmaster.ca Tue Apr 12 16:10:33 2005 From: lhotaks <@t> mcmaster.ca (Sarka Lhotak) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Re: Is TUNEL a waste of time? Message-ID: Dear Toshi, TUNEL is not a waste of time, just don't use the Roche kit! I had the same problems with it: lots of normal looking nuclei staining positive. Now I am using the Trevigen TACS kit (in Canada sold through BIO/CAN)and it works great. You can buy individual components, no need to get the whole kit each time you run out of a component. I buy the TdT enzyme, biotinylated dNTP nucleotides, Co 2+ cations, Proteinase K, TdT Labeling buffer and TdT Stop buffer. I follow their protocol up to the Stop buffer step, then I vizualize the incorporated biotinylated nucleotides either with my usual IHC Streptavidin-peroxidase, Nova-Red incubations, or for fluorescence, with Streptavidin-Alexa from Molecular Probes. The staining is very clean with nuclei of apoptotic morphology staining positive. You can check my double immunofluorescence for TUNEL and cleaved caspase-3 in mouse thymus in our recent paper: Circulation 2005;111:1814-1821, Supplemental on-line Figure 5. TUNEL specifically stained nuclei in cells that had cytoplasm positive for cleaved caspase-3. Sarka Lhotak McMaster University, Hamilton, Ontario From POWELL_SA <@t> Mercer.edu Tue Apr 12 16:16:05 2005 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] (no subject) In-Reply-To: <20050412204931.9311.qmail@web52704.mail.yahoo.com> Message-ID: <01LN0IXJFHZ48WZ983@Macon2.Mercer.edu> A histotech I know took the HT last weekend here and told me there was a lot more cytology than expected on hers. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gareth Davis Sent: Tuesday, April 12, 2005 3:50 PM To: Shawn Leslie; Histonet Subject: Re: [Histonet] (no subject) Hi, I took the HT computer test last December. One helpful hint I can give is to know everything about tissue idenitification, what stain is needed for that tissue, what may go wrong with stain for a specific tissue and how to identify mistakes. I saw so many images, and it really caught me offguard. I expected many, but not as many as I saw and the questions to the images were not what I expected. I did pass with a good score, but I was really surprised that I did. Good luck. Gareth Davis --- Shawn Leslie wrote: > > > To All, > > I have a trainee who is about to sit for her HT computer exam, and > wondered if anyone has taken it recently, and could offer some helpful > hints. Thanks > > Shawn Leslie > > Confidentiality Notice: This e-mail message, including any > attachments, may contain confidential information. The information is > intended only for the use of the individual(s) or entity named above. > If you are not the intended recipient, you are notified that any > disclosure, copying, distribution, or the taking of any action in > reliance on the contents of this e-mail information is prohibited. > If you have received this e-mail in error, please contact the sender > by reply e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbroomal <@t> NEMOURS.ORG Tue Apr 12 16:20:36 2005 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] (no subject) Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A79A9@wlmmsx01.nemours.org> I can attest to that too. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shirley Powell Sent: Tuesday, April 12, 2005 5:16 PM To: 'Gareth Davis'; 'Shawn Leslie'; 'Histonet' Subject: RE: [Histonet] (no subject) A histotech I know took the HT last weekend here and told me there was a lot more cytology than expected on hers. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gareth Davis Sent: Tuesday, April 12, 2005 3:50 PM To: Shawn Leslie; Histonet Subject: Re: [Histonet] (no subject) Hi, I took the HT computer test last December. One helpful hint I can give is to know everything about tissue idenitification, what stain is needed for that tissue, what may go wrong with stain for a specific tissue and how to identify mistakes. I saw so many images, and it really caught me offguard. I expected many, but not as many as I saw and the questions to the images were not what I expected. I did pass with a good score, but I was really surprised that I did. Good luck. Gareth Davis --- Shawn Leslie wrote: > > > To All, > > I have a trainee who is about to sit for her HT computer exam, and > wondered if anyone has taken it recently, and could offer some helpful > hints. Thanks > > Shawn Leslie > > Confidentiality Notice: This e-mail message, including any > attachments, may contain confidential information. The information is > intended only for the use of the individual(s) or entity named above. > If you are not the intended recipient, you are notified that any > disclosure, copying, distribution, or the taking of any action in > reliance on the contents of this e-mail information is prohibited. > If you have received this e-mail in error, please contact the sender > by reply e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters <@t> uiowa.edu Tue Apr 12 18:25:18 2005 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Re: Is TUNEL a waste of time? 3% citric acid step Message-ID: Hi Sarka, I pulled up your article and I noticed that you used a 3% citric acid step before the TUNEL stain for minimizing non-specific staining. What is the purpose of that step? (I tried to pull up the article that you referenced there, but our account only goes back to 1998). Thanks for any information you can give me. Kathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarka Lhotak Sent: Tuesday, April 12, 2005 4:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Is TUNEL a waste of time? Dear Toshi, TUNEL is not a waste of time, just don't use the Roche kit! I had the same problems with it: lots of normal looking nuclei staining positive. Now I am using the Trevigen TACS kit (in Canada sold through BIO/CAN)and it works great. You can buy individual components, no need to get the whole kit each time you run out of a component. I buy the TdT enzyme, biotinylated dNTP nucleotides, Co 2+ cations, Proteinase K, TdT Labeling buffer and TdT Stop buffer. I follow their protocol up to the Stop buffer step, then I vizualize the incorporated biotinylated nucleotides either with my usual IHC Streptavidin-peroxidase, Nova-Red incubations, or for fluorescence, with Streptavidin-Alexa from Molecular Probes. The staining is very clean with nuclei of apoptotic morphology staining positive. You can check my double immunofluorescence for TUNEL and cleaved caspase-3 in mouse thymus in our recent paper: Circulation 2005;111:1814-1821, Supplemental on-line Figure 5. TUNEL specifically stained nuclei in cells that had cytoplasm positive for cleaved caspase-3. Sarka Lhotak McMaster University, Hamilton, Ontario _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mail2melody <@t> yahoo.com Tue Apr 12 21:05:47 2005 From: mail2melody <@t> yahoo.com (Melody Thiessen) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] looking for suggestions on distiguishing protozoa. Message-ID: <20050413020547.16142.qmail@web42202.mail.yahoo.com> "Does someone have a suggestion for a stain that would distinguish bacteria from protozoa?" I checked with my Microbiology instructor. He said that usually bacteria and protozoa can be distinguished from one another based on size alone (protozoa are considerably larger than bacteria). Shape can also indicate as bacteria are usually one of the three major shapes: coccus (round), bacillus (rod), or spirochete (like a snake). Most standard micro stains (crystal violet, methylene blue, etc.) should stain both bacteria and protozoa enough to see both clearly. I hope that helps a little. -Melody Thiessen (HT student) --------------------------------- Yahoo! Mail Mobile Take Yahoo! Mail with you! Check email on your mobile phone. From Krat18 <@t> aol.com Wed Apr 13 03:40:30 2005 From: Krat18 <@t> aol.com (Krat18@aol.com) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Cost of automated immunos? Message-ID: <104.5f022af6.2f8e34fe@aol.com> Our lab is starting to think about doing automated immunos. Can anyone out there is Histoland give me an idea of the COST of doing an automated immuno vs. a manual one? I know there are many variables, but we're looking at the Benchmark, so any idea of the cost per test on that machine? We would appreciate any input. Thanks in advance! Chris Davis _chrisdavis@hotmail.com_ (mailto:chrisdavis@hotmail.com) From Krat18 <@t> aol.com Wed Apr 13 04:06:52 2005 From: Krat18 <@t> aol.com (Krat18@aol.com) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Cost per test of doing immunos? Message-ID: <1a9.35caa85c.2f8e3b2c@aol.com> Our lab is starting to think about doing automated immunos. Can anyone out there in Histoland give me an idea of the COST PER TEST of doing an automated immuno vs. a manual one? I know there are many variables, but we're looking at the Ventana Benchmark, so any idea of the cost per test on that machine? We would appreciate any input. Thanks in advance! Chris Davis _chrisdavis1012@hotmail.com_ (mailto:chrisdavis1012@hotmail.com) (Sorry, there was an error in the address in my last message.) From TillRenee <@t> uams.edu Wed Apr 13 07:26:24 2005 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] controls and secondaries Message-ID: What is the difference between a secondary such as a goat anti- mouse and a straight mouse IgG istotype control as far as it's applications in the IHC process? I ask because someone from another lab recently came to me for help with a long list of stains they need done in a short period of time. They have already ordered everything they think they need to complete the stains. Most of them are flouescent conjugated primaries, but two will have to have the fluorescence attached to the secondary. I am wondering about this because I was asked why their protocol would call specifically for goat serum to block, and would it matter if it was another species. I told them that usually the blocking serum is the same as the species the secondary was made in and was told that the secondary wasn't made in anything different such as a goat anti-mouse, they had what I would normally use as control serum. Can it be used as a secondary? I have never tried this. Supposedly it worked with their flow samples as a secondary (these are mouse primaries on pig tissue). Renee' Till, HT Arkansas Childrens Nutrition Center 1120 Marshall Little Rock, AR 72202 (501) 364-2774 From dahmed <@t> mdanderson.org Wed Apr 13 07:36:44 2005 From: dahmed <@t> mdanderson.org (dahmed@mdanderson.org) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] (no subject) Message-ID: Does anyone use Isopropyl Alcohol instead of Ethyl Alcohol on their processor? Does anyone feel it does a better job at dehydrating the tissue? At the request of the pathologists, I am looking for any method that will speed up the processing time for breast tissue. Any information and/or suggestion is welcome. Thank you. David S. Ahmed Chief Histology Technician Department of Pathology M.D. Anderson Cancer Center From la.sebree <@t> hosp.wisc.edu Wed Apr 13 07:49:19 2005 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Cost of automated immunos? Message-ID: Chris, I suggest contacting Ventana, they can give you a pretty accurate cost estimate per slide. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Krat18@aol.com Sent: Wednesday, April 13, 2005 3:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost of automated immunos? Our lab is starting to think about doing automated immunos. Can anyone out there is Histoland give me an idea of the COST of doing an automated immuno vs. a manual one? I know there are many variables, but we're looking at the Benchmark, so any idea of the cost per test on that machine? We would appreciate any input. Thanks in advance! Chris Davis _chrisdavis@hotmail.com_ (mailto:chrisdavis@hotmail.com) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Wed Apr 13 07:44:09 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Isopropyl (again) Message-ID: If anyone ever came up with the brilliant idea of producing an FAQ, this would certainly be in the top 10 :-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: dahmed@mdanderson.org [mailto:dahmed@mdanderson.org] Sent: 13 April 2005 13:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Does anyone use Isopropyl Alcohol instead of Ethyl Alcohol on their processor? Does anyone feel it does a better job at dehydrating the tissue? At the request of the pathologists, I am looking for any method that will speed up the processing time for breast tissue. Any information and/or suggestion is welcome. Thank you. David S. Ahmed Chief Histology Technician Department of Pathology M.D. Anderson Cancer Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dndsomi <@t> aol.com Wed Apr 13 08:48:30 2005 From: dndsomi <@t> aol.com (dndsomi@aol.com) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Microscope Service Message-ID: <8C70E469C9E2B5D-9E0-26B71@mblk-r21.sysops.aol.com> We are trying to find a microscope service person in the East Tennessee area. Does anyone have contact information? Thanks. DDietz HT From petepath <@t> yahoo.com Wed Apr 13 09:06:41 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Isopropyl (again) Message-ID: <20050413140642.59674.qmail@web30403.mail.mud.yahoo.com> I cannot help you with the isopropal question. But let me ask you are you trying to speed up your turn around time on breasts or are you having trouble cutting under processed tissue? If you are trying to speed up turn around time you picked a bad tissue to be in a rush with! Reading a miserably processed breast specimen is a frustrating and risky buisness frought with potential legal repercussions. I think your pathologists would agree with this. I suggest a little patience. If the surgeons are busting their chops it is time for a little histology lesson for the surgeons. I like to tell my surgeons I will be be happy to give a rush diagnosis, but if they would like the correct diagnosis that will take a bit longer! If poorly processed tissue is the problem then it is a matter of all the things you already know. The sections should be cut no more than 3mm thick across the entire section ( not just the edge). Once cut sections should be give adequate fixation in clean formalin before putting it in the processor. Over night if possible. Processor solutions should be clean and changed at appropriate intervals. Baskets should not be overstuffed if possible especially with, cassettes filled with large samples. If you have multiple processors at your disposal you may want to sequester these cases an adjust the program and solution changes until you are satisfied with the result. If your grossing staff leaves intact specimen sit overnight in the fixative it arrived in and then cuts it the next day, fixation will often not be adequate because inadequate amout of formalin, formalin which has been weakened by dilution or inability of the formalin to penetrate and fix a thick piece of tissue. I am sure you already know this . Make sure your grossing staff do to. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From JWEEMS <@t> sjha.org Wed Apr 13 09:13:12 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Cost of automated immunos? Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA45947@sjhaexc02.sjha.org> Also, DAKO. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sebree Linda A. Sent: Wednesday, April 13, 2005 8:49 AM To: Krat18@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cost of automated immunos? Chris, I suggest contacting Ventana, they can give you a pretty accurate cost estimate per slide. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Krat18@aol.com Sent: Wednesday, April 13, 2005 3:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost of automated immunos? Our lab is starting to think about doing automated immunos. Can anyone out there is Histoland give me an idea of the COST of doing an automated immuno vs. a manual one? I know there are many variables, but we're looking at the Benchmark, so any idea of the cost per test on that machine? We would appreciate any input. Thanks in advance! Chris Davis _chrisdavis@hotmail.com_ (mailto:chrisdavis@hotmail.com) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From sluhisto <@t> yahoo.com Wed Apr 13 09:17:47 2005 From: sluhisto <@t> yahoo.com (Histology SLU) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Cost of automated immunos? In-Reply-To: 6667 Message-ID: <20050413141747.63770.qmail@web51006.mail.yahoo.com> I second DAKO. "Weems, Joyce" wrote:Also, DAKO. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sebree Linda A. Sent: Wednesday, April 13, 2005 8:49 AM To: Krat18@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cost of automated immunos? Chris, I suggest contacting Ventana, they can give you a pretty accurate cost estimate per slide. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Krat18@aol.com Sent: Wednesday, April 13, 2005 3:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost of automated immunos? Our lab is starting to think about doing automated immunos. Can anyone out there is Histoland give me an idea of the COST of doing an automated immuno vs. a manual one? I know there are many variables, but we're looking at the Benchmark, so any idea of the cost per test on that machine? We would appreciate any input. Thanks in advance! Chris Davis _chrisdavis@hotmail.com_ (mailto:chrisdavis@hotmail.com) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From llewllew <@t> shaw.ca Wed Apr 13 09:35:33 2005 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] isopropanol References: Message-ID: <002001c54036$0d0e9bc0$7e034246@yourlk4rlmsu> I used isopropanol quite a bit years ago. It dehydrates satisfactorily, although it is not as efficient as absolute ethanol. It leaves the tissues a little more brittle than ethanol does, so the sectioning is slightly different. I never found any difference in speed, certainly not to the degree you appear to be looking for. Our reasons for using it had to do with the problems getting a license for using ethanol in Britain at that time. In essence, any time you speed up processing, particularly for fatty tissues, you will reduce quality. That is usually the trade off. Cutting thinner blocks and using a fast fixative (like Carnoy or Clark) are probably the only things that might help, and fixatives like that have their own issues regarding the quality of the morphology in the finished preparation. Essentially, what you are looking for has been done decades ago, and the times and procedures we use now is the result. Bryan Llewellyn ----- Original Message ----- From: To: Sent: Wednesday, April 13, 2005 5:36 AM Subject: [Histonet] (no subject) > Does anyone use Isopropyl Alcohol instead of Ethyl Alcohol on their > processor? Does anyone feel it does a better job at dehydrating the > tissue? At the request of the pathologists, I am looking for any method > that will speed up the processing time for breast tissue. Any information > and/or suggestion is welcome. > > Thank you. > > > David S. Ahmed > Chief Histology Technician > Department of Pathology > M.D. Anderson Cancer Center > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gcallis <@t> montana.edu Wed Apr 13 09:46:16 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Eudora and no email messages Message-ID: <6.0.0.22.1.20050413084021.01b12a30@gemini.msu.montana.edu> Is something wrong with the system? I have received no emails via Eudora since late yesterday afternoon. Nothing comes in when I open the "Check Mail" box. Extension is 6367 Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Janet.Bonner <@t> FLHOSP.ORG Wed Apr 13 09:46:39 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] isopropanol Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4235@fh2k093.fhmis.net> AND who knows what it does to the reactivity of the IHC staining... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: Histonet Sent: 4/13/2005 10:35 AM Subject: [Histonet] isopropanol I used isopropanol quite a bit years ago. It dehydrates satisfactorily, although it is not as efficient as absolute ethanol. It leaves the tissues a little more brittle than ethanol does, so the sectioning is slightly different. I never found any difference in speed, certainly not to the degree you appear to be looking for. Our reasons for using it had to do with the problems getting a license for using ethanol in Britain at that time. In essence, any time you speed up processing, particularly for fatty tissues, you will reduce quality. That is usually the trade off. Cutting thinner blocks and using a fast fixative (like Carnoy or Clark) are probably the only things that might help, and fixatives like that have their own issues regarding the quality of the morphology in the finished preparation. Essentially, what you are looking for has been done decades ago, and the times and procedures we use now is the result. Bryan Llewellyn ----- Original Message ----- From: To: Sent: Wednesday, April 13, 2005 5:36 AM Subject: [Histonet] (no subject) > Does anyone use Isopropyl Alcohol instead of Ethyl Alcohol on their > processor? Does anyone feel it does a better job at dehydrating the > tissue? At the request of the pathologists, I am looking for any method > that will speed up the processing time for breast tissue. Any information > and/or suggestion is welcome. > > Thank you. > > > David S. Ahmed > Chief Histology Technician > Department of Pathology > M.D. Anderson Cancer Center > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mclarke <@t> allsaintshealthcare.org Wed Apr 13 09:48:33 2005 From: mclarke <@t> allsaintshealthcare.org (Clarke, Mary) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] RE: Formula 83 Message-ID: <3959B2255CA51443928A90517C973D6418EC63@WFEXBE04.wfsi.priv> We used the Formula 83 for about a year and we were happy with it. Terri Clarke Histology Supervisor All Saints Laboratory 3801 Spring Street Racine, Wi 53405 mclarke@allsaintshealthcare.org 262-687-6609 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, April 12, 2005 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 17, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Immunohistochemical staining interference by ethanol vs alcohol blend (Malek, Jack M) 2. formula 83 (Sue Barnes) 3. Ethanol Interference with Immunohistochemical staining (Malek, Jack M) 4. looking for suggestions on distiguishing protozoa. (HCS) 5. p16 source (Luis Chiriboga) 6. RE: Is TUNEL a waste of time? (Satoshi Akima) 7. Re: Tissue Micro Arrayer (Histo Jock) 8. Embedding Center (Steven Coakley) 9. RE: formula 83 (Christine Tambasco) 10. Re: microtome question (Christine Tambasco) 11. Re: microtome question (Jacqueline Lair) 12. Formula 83 (Kerry Patience) 13. Delaware diamond knives (Burns, Julie) 14. Porcine macrophages (Jens Lindegaard) 15. WT1 (Prada-Puentes Carlos (RW6) PAHNT) 16. Known Gastrin Controls (Histology SLU) 17. vendors (Kapoor, Sue) 18. RE: Porcine macrophages (Patsy Ruegg) 19. Re: vendors (meyenhmf@umdnj.edu) 20. Recycling (Rush, Joyce) 21. Tape transfer sections and IHC (Patsy Ruegg) 22. RE: Recycling (Connie Sims) 23. RE: Porcine macrophages (Favara, Cynthia (NIH/NIAID)) ---------------------------------------------------------------------- Message: 1 Date: Mon, 11 Apr 2005 11:09:47 -0700 From: "Malek, Jack M" Subject: [Histonet] Immunohistochemical staining interference by ethanol vs alcohol blend To: Message-ID: <63EB2FB8C50AD242898B76E2B2C7C12202121AC6@ROC2T9.ghc.org> Content-Type: text/plain; charset="us-ascii" Has anyone experienced interference with immunohistochemical staining when using an ethanol blend versus an alcohol blend? We are preparing to switch from Richard Allan's Flex 100 (isopropanol/methanol blend) to their Dehydrant (ethanol/isopropanol/methanol blend). Thank you in advance, Jack Malek GHC Pathology Seattle, Washington 206-326-3251 Malek.j@ghc.org ------------------------------ Message: 2 Date: Mon, 11 Apr 2005 14:58:49 -0400 From: "Sue Barnes" Subject: [Histonet] formula 83 To: Message-ID: Content-Type: text/plain; charset="Windows-1252" Hi, Has anyone had any experience with the formula 83 replacement for xylene? Received a flyer on it and was wondering if anyone was using it yet. Sue Barnes CT,HT (ASCP) sbarnes@elch.org East Liverpool City Hospital East Liverpool, Ohio ------------------------------ Message: 3 Date: Mon, 11 Apr 2005 12:16:30 -0700 From: "Malek, Jack M" Subject: [Histonet] Ethanol Interference with Immunohistochemical staining To: Message-ID: <63EB2FB8C50AD242898B76E2B2C7C12202B12539@ROC2T9.ghc.org> Content-Type: text/plain; charset="us-ascii" Has anyone experienced interference with immunohistochemical staining when using an ethanol blend versus an alcohol blend? We are preparing to switch from Richard Allan's Flex 100 (isopropanol/methanol blend) to their Dehydrant (ethanol/isopropanol/methanol blend). Thank you in advance, Jack Malek GHC Pathology Seattle, Washington 206-326-3251 Malek.j@ghc.org ------------------------------ Message: 4 Date: Mon, 11 Apr 2005 13:12:15 -0700 From: "HCS" Subject: [Histonet] looking for suggestions on distiguishing protozoa. To: "Histonet" Message-ID: <000601c53ed2$c1e23990$6601a8c0@hp> Content-Type: text/plain; charset="iso-8859-1" Does someone have a suggestion for a stain that would distinguish bacteria from protozoa? Thanks in advance LeRoy Brown HCS www.histocs.com 360-966-7300 ------------------------------ Message: 5 Date: Mon, 11 Apr 2005 10:20:26 -0400 From: Luis Chiriboga Subject: [Histonet] p16 source To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi everyone Can anyone recommend a source for anti-p16 for use in human frozen tissue.......? TIA `Luis ------------------------------ Message: 6 Date: Tue, 12 Apr 2005 08:35:27 +1000 From: Satoshi Akima Subject: [Histonet] RE: Is TUNEL a waste of time? To: histonet@lists.utsouthwestern.edu Message-ID: <0787a008ede14e834647a585c31a69e2@bigpond.net.au> Content-Type: text/plain; charset=US-ASCII; format=flowed On 11/04/2005, at 7:54 PM, Edwards, R.E. wrote: > We abandoned the TUNEL method, many years ago as we felt it > gave too many false +ves, we used H@E staining until reliable > antibodies came along, e.g., caspase 3............ I have found others who use TUNEL staining express a similar opinion about the false positives. I would be interested to know if others too have found an excessive rate of false positives. Still the TUNEL method seems to be viewed by a great many as some sort of Gold Standard. Many find still swear by it as a very good tool. Regarding the issue of specificity - does caspase-3 also help to distinguish between apoptosis and necrosis I wonder? Toshi Akima PhD Student Centre for Transplantation and Renal Research Westmead Millenium Institute Sydney, Australia Toshi ------------------------------ Message: 7 Date: Mon, 11 Apr 2005 18:41:48 -0400 From: "Histo Jock" Subject: [Histonet] Re: Tissue Micro Arrayer To: histonet@lists.utsouthwestern.edu Cc: Charlene.Henry@STJUDE.ORG Message-ID: Content-Type: text/plain; format=flowed Hi Charlene, I assume that you ordered a Beecher MTA-II? If so you should expect a wait. Beecher's orders for that model are running months ahead of production. I understand that it's been a very popular machine. If you ask them nicely they will send you an MTA-I to use until your MTA-II is ready or you can just change the order to an MTA-I. For most labs the MTA-I is more than you need anyway. Beecher normally has long wait times for everything they make. Beecher is the only source for tissue arrayers as they own all of the patents on them. Chemicon makes an arrayer by licensing a design from Beecher but as noted already it is both inferior to and hugely more expensive than the Beecher models ($60K!!! Chemicon vs. $10-20k Beecher). Nobody who really knows tissue arrays buys the Chemicon. I've never heard anybody speak highly of it. HistoJock >Hi Folks, >Does anyone know of another vendor that makes the tissue micro >arrayer other than Beecher Instrument. I placed an order with >Beecher >Instrument in November, 2004 and they are telling us that it will still be >2-3 >months before it will be shipped. At this point I'm willing to look for >another vendor. >Thanks, > >Charlene Henry HT (ASCP), QIHC _________________________________________________________________ Don't just search. Find. Check out the new MSN Search! http://search.msn.click-url.com/go/onm00200636ave/direct/01/ ------------------------------ Message: 8 Date: Mon, 11 Apr 2005 18:02:12 -0500 From: "Steven Coakley" Subject: [Histonet] Embedding Center To: Message-ID: <003901c53eea$8f6bc040$5e81b542@oemcomputer> Content-Type: text/plain; charset="iso-8859-1" Our Labs looking for a smaller size used or refurbished embedding center. Thanks ------------------------------ Message: 9 Date: Tue, 12 Apr 2005 00:29:34 +0000 From: "Christine Tambasco" Subject: RE: [Histonet] formula 83 To: SBarnes@elch.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format="flowed" We've been using it now for about a month. We tried it about a year ago and finally got approval to switch. I love it! It is almost as good as xylene. Good Luck! Christine Tambasco, HT (ASCP) ST. Mary's Hospital 427 Guy Park Ave Amsterdam, New York 12010 >From: "Sue Barnes" >To: >Subject: [Histonet] formula 83 >Date: Mon, 11 Apr 2005 14:58:49 -0400 > >Hi, >Has anyone had any experience with the formula 83 replacement for xylene? > >Received a flyer on it and was wondering if anyone was using it yet. > >Sue Barnes CT,HT (ASCP) > >sbarnes@elch.org >East Liverpool City Hospital >East Liverpool, Ohio > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 12 Apr 2005 00:40:02 +0000 From: "Christine Tambasco" Subject: Re: [Histonet] microtome question To: Vickroy.Jim@mhsil.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format="flowed" maybe it's the cassettes that are not fitting right? It's possible to get a bad batch. Try it and good luck! Christine Tambasco, HT (ASCP) St. Mary's Hospital @ Amsterdam, New York >From: RCHIOVETTI@aol.com >To: Vickroy.Jim@mhsil.com, histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] microtome question >Date: Fri, 8 Apr 2005 16:25:21 EDT > >In a message dated 4/8/2005 1:07:44 PM US Mountain Standard Time, >Vickroy.Jim@mhsil.com writes: > > > we are having > > some problems with the block holders not holding the tissue firm enough > > and therefore the blocks can move and therefore chunk. We have tried a > > couple of new holders but are experiencing the same problem. > >Jim, > >You may already have ruled this out, but I wonder if the problem could be in >the knife holder instead of the cassette clamp? It's strange that you get the >same problem with more than one new specimen holder...nless there was some >kind of manufacturing defect that went undetected, I guess. > >My Two Cents' Worth! > >Cheers, > >Bob > >Robert (Bob) Chiovetti, Ph.D. >Independent Consultant for The Science, Technology and Industrial Sectors >132 North Elster Drive >Tucson, AZ 85710-3212 USA >Tel./Fax 520-546-4986 >Member, Arizona Small Business Association - ASBA >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 11 Apr 2005 18:59:37 -0700 (PDT) From: Jacqueline Lair Subject: Re: [Histonet] microtome question To: histonet@lists.utsouthwestern.edu Message-ID: <20050412015938.34862.qmail@web81509.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii I was having a similar problem with the microtomes in our lab.. the block would chunk out the tissue and the block seemed as if it were barely held into the block holder, very "wobbly like". You could wiggle the block around in the block holder after it was clamped in. This is what I did: Look at the block holder as its held or in the "open" position you would need to have it to insert a block. You should see one or two small openings (the "openings" will either be on the bottom or on the sides of the block holder, depending on which type of microtome you are using.) between the metal on the block holder; i.e. where the metal would come or clamp together to hold a paraffin block. Next, take a pick and scrape the paraffin out of the "opening(s)" in the block holder. When I did the scraping, my block holder had a bunch of paraffin built up "inside" of it, which was causing the block holder to not clamp down as tightly or securely on the block. I find that if I face in many blocks at one time, I have to scrape out the block holder after Ive finished all of my facing. Now, Ive just gotten into the habit of scraping out the block holder after Im done facing to ensure that there is no paraffin build up within the block holder at all. I hope this makes sense? I hope it helps. Jacqueline Lair, HT (ASCP) ------------------------------ Message: 12 Date: Tue, 12 Apr 2005 17:25:56 +1200 From: "Kerry Patience" Subject: [Histonet] Formula 83 To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Is formula 83 a xylene substitute? What company produces it and how does the price compare to xylene? ------------------------------ Message: 13 Date: Tue, 12 Apr 2005 09:29:02 +0100 From: "Burns, Julie" Subject: [Histonet] Delaware diamond knives To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi I work in a busy electron microscopy unit and I am considering the purchase of a delaware diamond knive - I was wanting to know of anyones experiences of these diamond knives ? many thanks Julie Burns ------------------------------ Message: 14 Date: Tue, 12 Apr 2005 15:13:35 +0200 From: "Jens Lindegaard" Subject: [Histonet] Porcine macrophages To: histonet@lists.utsouthwestern.edu Message-ID: Hello Can anyone tell me which antibody to use in order to immunostain porcine macrophages in paraffin embedded sections? Jens Lindegaard M.D. Eye Pathology Institute Frederik V's Vej 11, 1.floor DK-2100 Copenhagen Denmark Phone +45 3532 6083 Fax +45 3532 6080 ------------------------------ Message: 15 Date: Tue, 12 Apr 2005 14:59:39 +0100 From: "Prada-Puentes Carlos \(RW6\) PAHNT" Subject: [Histonet] WT1 To: "HISTONET" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi there, I have been using WT1 with good results from Santa Cruz biotechnology. My problem is that after 6 months the antibody has become unstable and altough we have concentrated it the nuclear staining is weaker and weaker. The datasheet does not recommend freezing and gives ONLY ONE YEAR!!!! Of guaranteed stability kept at 4 C..Should I freeze it when the new vial comes? Considering we use at a dilution of 1/3000 we need to freeze before we use it all. Thank you to all Dr Carlos Prada-Puentes ------------------------------ Message: 16 Date: Tue, 12 Apr 2005 07:01:15 -0700 (PDT) From: Histology SLU Subject: [Histonet] Known Gastrin Controls To: histonet@lists.utsouthwestern.edu Message-ID: <20050412140115.83247.qmail@web51007.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Hello All: Does anyone have any extra human gastrin IHC control blocks that you can spare? Thanks in advance for your help and have a wonderful day! Susan --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! ------------------------------ Message: 17 Date: Tue, 12 Apr 2005 10:01:14 -0500 From: "Kapoor, Sue" Subject: [Histonet] vendors To: histonet@lists.utsouthwestern.edu Message-ID: <61E9F2400F53D5119CFC00508B44E33B02E9E0D1@khmcexch.uhsi.org> Content-Type: text/plain; charset=iso-8859-1 To all vendors, I'm looking for a used fume hood for a Tissue-Tek DRS-601 slide stainer and a used Tissue-Tek Cryo Console. Please email me at: sue.kapoor@uhsi.org Thank you, Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 ------------------------------ Message: 18 Date: Tue, 12 Apr 2005 09:46:35 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] Porcine macrophages To: "'Jens Lindegaard'" , Message-ID: <200504121546.j3CFkQOD004555@chip.viawest.net> Content-Type: text/plain; charset="US-ASCII" I use RDI-Macropabm-387 (Mac387) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jens Lindegaard Sent: Tuesday, April 12, 2005 6:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Porcine macrophages Hello Can anyone tell me which antibody to use in order to immunostain porcine macrophages in paraffin embedded sections? Jens Lindegaard M.D. Eye Pathology Institute Frederik V's Vej 11, 1.floor DK-2100 Copenhagen Denmark Phone +45 3532 6083 Fax +45 3532 6080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Tue, 12 Apr 2005 11:49:02 -0400 From: meyenhmf@umdnj.edu Subject: Re: [Histonet] vendors To: "Kapoor, Sue" Cc: histonet@lists.utsouthwestern.edu Message-ID: <1113320942.425bedeee6f1a@webmail.umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1 I have a slightly used (1 year in a small hospital) ^)! with hood available. Also lots of other EM, Histo and Lab Equipment. Please contact me at: micro@superlink.net Regards, Markus F. Meyenhofer Microscopy Labs micro@superlink.net Quoting "Kapoor, Sue" : > To all vendors, I'm looking for a used fume hood for a Tissue-Tek > DRS-601 > slide stainer and a used Tissue-Tek Cryo Console. > > Please email me at: > sue.kapoor@uhsi.org > > Thank you, > Sue Kapoor, HT (ASCP) > Histology Coordinator > Kenosha Medical Center > Kenosha, WI > 262-653-5570 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- ------------------------------ Message: 20 Date: Tue, 12 Apr 2005 10:55:04 -0500 From: "Rush, Joyce" Subject: [Histonet] Recycling To: Message-ID: <2337362E8548BE4C85EE5DD5D526B0851942B4@sjw3smail2.SJMCMN.ORG> Content-Type: text/plain; charset="iso-8859-1" We are looking into recycling our alcohol, formalin and Americlear. I'd love to hear any experiences doing this. We are a relatively small user and currently sewer everything. I'm trying to gather information for the day when we can not do this. Thanks, Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 ------------------------------ Message: 21 Date: Tue, 12 Apr 2005 10:11:10 -0600 From: "Patsy Ruegg" Subject: [Histonet] Tape transfer sections and IHC To: Message-ID: <200504121611.j3CGB1OD014811@chip.viawest.net> Content-Type: text/plain; charset="US-ASCII" I am doing hrp IHC on tape transfer sections and I am experiencing a problem with unwanted staining on the coating on the polymer coated slides. Does anyone else out there do IHC on the tape sections experience this problem? Please suggest a solution to this. Thank you, Patsy ------------------------------ Message: 22 Date: Tue, 12 Apr 2005 11:18:39 -0500 From: "Connie Sims" Subject: RE: [Histonet] Recycling To: histonet@lists.utsouthwestern.edu Message-ID: <11906C81B7B03E47A6F10CE5F4784449018A9C1F@dchexch01.driscoll.dch> Content-Type: text/plain; charset=us-ascii We currently have a CBG recycler. I love it! It does take some tech time and planning but the cost savings and environmental benefits are worth it. Formalin requires rebuffering and alcohol is only 95%. Americlear is completely reuseable. I haven't had any problems so far, its been almost a year now. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rush, Joyce Sent: Tuesday, April 12, 2005 10:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling We are looking into recycling our alcohol, formalin and Americlear. I'd love to hear any experiences doing this. We are a relatively small user and currently sewer everything. I'm trying to gather information for the day when we can not do this. Thanks, Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================================== ================================ Driscoll Children's Hospital Disclaimer: This e-mail message and all information enclosed, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution of the information enclosed, in its entirety or in part, is strictly prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and immediately destroy all copies of the message. For further assistance or questions please contact the email administrator at Driscoll Children's Hospital at (361) 694-5000. ======================================================================== ================================= ------------------------------ Message: 23 Date: Tue, 12 Apr 2005 12:52:37 -0400 From: "Favara, Cynthia (NIH/NIAID)" Subject: RE: [Histonet] Porcine macrophages To: 'Patsy Ruegg' , 'Jens Lindegaard' , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain Have you looked into F4/80? c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Tuesday, April 12, 2005 8:47 AM To: 'Jens Lindegaard'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Porcine macrophages I use RDI-Macropabm-387 (Mac387) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jens Lindegaard Sent: Tuesday, April 12, 2005 6:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Porcine macrophages Hello Can anyone tell me which antibody to use in order to immunostain porcine macrophages in paraffin embedded sections? Jens Lindegaard M.D. Eye Pathology Institute Frederik V's Vej 11, 1.floor DK-2100 Copenhagen Denmark Phone +45 3532 6083 Fax +45 3532 6080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 17, Issue 20 **************************************** From mclarke <@t> allsaintshealthcare.org Wed Apr 13 09:50:13 2005 From: mclarke <@t> allsaintshealthcare.org (Clarke, Mary) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] RE: Recycler Message-ID: <3959B2255CA51443928A90517C973D6418EC64@WFEXBE04.wfsi.priv> We have been using the CBG recycler for about 5 years and have had no problems with it so far. The recycled product works great and it is a great cost saver. Terri Clarke Histology Supervisor All Saints Laboratory 3801 Spring Street Racine, Wi 53405 mclarke@allsaintshealthcare.org 262-687-6609 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, April 12, 2005 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 17, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Immunohistochemical staining interference by ethanol vs alcohol blend (Malek, Jack M) 2. formula 83 (Sue Barnes) 3. Ethanol Interference with Immunohistochemical staining (Malek, Jack M) 4. looking for suggestions on distiguishing protozoa. (HCS) 5. p16 source (Luis Chiriboga) 6. RE: Is TUNEL a waste of time? (Satoshi Akima) 7. Re: Tissue Micro Arrayer (Histo Jock) 8. Embedding Center (Steven Coakley) 9. RE: formula 83 (Christine Tambasco) 10. Re: microtome question (Christine Tambasco) 11. Re: microtome question (Jacqueline Lair) 12. Formula 83 (Kerry Patience) 13. Delaware diamond knives (Burns, Julie) 14. Porcine macrophages (Jens Lindegaard) 15. WT1 (Prada-Puentes Carlos (RW6) PAHNT) 16. Known Gastrin Controls (Histology SLU) 17. vendors (Kapoor, Sue) 18. RE: Porcine macrophages (Patsy Ruegg) 19. Re: vendors (meyenhmf@umdnj.edu) 20. Recycling (Rush, Joyce) 21. Tape transfer sections and IHC (Patsy Ruegg) 22. RE: Recycling (Connie Sims) 23. RE: Porcine macrophages (Favara, Cynthia (NIH/NIAID)) ---------------------------------------------------------------------- Message: 1 Date: Mon, 11 Apr 2005 11:09:47 -0700 From: "Malek, Jack M" Subject: [Histonet] Immunohistochemical staining interference by ethanol vs alcohol blend To: Message-ID: <63EB2FB8C50AD242898B76E2B2C7C12202121AC6@ROC2T9.ghc.org> Content-Type: text/plain; charset="us-ascii" Has anyone experienced interference with immunohistochemical staining when using an ethanol blend versus an alcohol blend? We are preparing to switch from Richard Allan's Flex 100 (isopropanol/methanol blend) to their Dehydrant (ethanol/isopropanol/methanol blend). Thank you in advance, Jack Malek GHC Pathology Seattle, Washington 206-326-3251 Malek.j@ghc.org ------------------------------ Message: 2 Date: Mon, 11 Apr 2005 14:58:49 -0400 From: "Sue Barnes" Subject: [Histonet] formula 83 To: Message-ID: Content-Type: text/plain; charset="Windows-1252" Hi, Has anyone had any experience with the formula 83 replacement for xylene? Received a flyer on it and was wondering if anyone was using it yet. Sue Barnes CT,HT (ASCP) sbarnes@elch.org East Liverpool City Hospital East Liverpool, Ohio ------------------------------ Message: 3 Date: Mon, 11 Apr 2005 12:16:30 -0700 From: "Malek, Jack M" Subject: [Histonet] Ethanol Interference with Immunohistochemical staining To: Message-ID: <63EB2FB8C50AD242898B76E2B2C7C12202B12539@ROC2T9.ghc.org> Content-Type: text/plain; charset="us-ascii" Has anyone experienced interference with immunohistochemical staining when using an ethanol blend versus an alcohol blend? We are preparing to switch from Richard Allan's Flex 100 (isopropanol/methanol blend) to their Dehydrant (ethanol/isopropanol/methanol blend). Thank you in advance, Jack Malek GHC Pathology Seattle, Washington 206-326-3251 Malek.j@ghc.org ------------------------------ Message: 4 Date: Mon, 11 Apr 2005 13:12:15 -0700 From: "HCS" Subject: [Histonet] looking for suggestions on distiguishing protozoa. To: "Histonet" Message-ID: <000601c53ed2$c1e23990$6601a8c0@hp> Content-Type: text/plain; charset="iso-8859-1" Does someone have a suggestion for a stain that would distinguish bacteria from protozoa? Thanks in advance LeRoy Brown HCS www.histocs.com 360-966-7300 ------------------------------ Message: 5 Date: Mon, 11 Apr 2005 10:20:26 -0400 From: Luis Chiriboga Subject: [Histonet] p16 source To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi everyone Can anyone recommend a source for anti-p16 for use in human frozen tissue.......? TIA `Luis ------------------------------ Message: 6 Date: Tue, 12 Apr 2005 08:35:27 +1000 From: Satoshi Akima Subject: [Histonet] RE: Is TUNEL a waste of time? To: histonet@lists.utsouthwestern.edu Message-ID: <0787a008ede14e834647a585c31a69e2@bigpond.net.au> Content-Type: text/plain; charset=US-ASCII; format=flowed On 11/04/2005, at 7:54 PM, Edwards, R.E. wrote: > We abandoned the TUNEL method, many years ago as we felt it > gave too many false +ves, we used H@E staining until reliable > antibodies came along, e.g., caspase 3............ I have found others who use TUNEL staining express a similar opinion about the false positives. I would be interested to know if others too have found an excessive rate of false positives. Still the TUNEL method seems to be viewed by a great many as some sort of Gold Standard. Many find still swear by it as a very good tool. Regarding the issue of specificity - does caspase-3 also help to distinguish between apoptosis and necrosis I wonder? Toshi Akima PhD Student Centre for Transplantation and Renal Research Westmead Millenium Institute Sydney, Australia Toshi ------------------------------ Message: 7 Date: Mon, 11 Apr 2005 18:41:48 -0400 From: "Histo Jock" Subject: [Histonet] Re: Tissue Micro Arrayer To: histonet@lists.utsouthwestern.edu Cc: Charlene.Henry@STJUDE.ORG Message-ID: Content-Type: text/plain; format=flowed Hi Charlene, I assume that you ordered a Beecher MTA-II? If so you should expect a wait. Beecher's orders for that model are running months ahead of production. I understand that it's been a very popular machine. If you ask them nicely they will send you an MTA-I to use until your MTA-II is ready or you can just change the order to an MTA-I. For most labs the MTA-I is more than you need anyway. Beecher normally has long wait times for everything they make. Beecher is the only source for tissue arrayers as they own all of the patents on them. Chemicon makes an arrayer by licensing a design from Beecher but as noted already it is both inferior to and hugely more expensive than the Beecher models ($60K!!! Chemicon vs. $10-20k Beecher). Nobody who really knows tissue arrays buys the Chemicon. I've never heard anybody speak highly of it. HistoJock >Hi Folks, >Does anyone know of another vendor that makes the tissue micro >arrayer other than Beecher Instrument. I placed an order with >Beecher >Instrument in November, 2004 and they are telling us that it will still be >2-3 >months before it will be shipped. At this point I'm willing to look for >another vendor. >Thanks, > >Charlene Henry HT (ASCP), QIHC _________________________________________________________________ Don't just search. Find. Check out the new MSN Search! http://search.msn.click-url.com/go/onm00200636ave/direct/01/ ------------------------------ Message: 8 Date: Mon, 11 Apr 2005 18:02:12 -0500 From: "Steven Coakley" Subject: [Histonet] Embedding Center To: Message-ID: <003901c53eea$8f6bc040$5e81b542@oemcomputer> Content-Type: text/plain; charset="iso-8859-1" Our Labs looking for a smaller size used or refurbished embedding center. Thanks ------------------------------ Message: 9 Date: Tue, 12 Apr 2005 00:29:34 +0000 From: "Christine Tambasco" Subject: RE: [Histonet] formula 83 To: SBarnes@elch.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format="flowed" We've been using it now for about a month. We tried it about a year ago and finally got approval to switch. I love it! It is almost as good as xylene. Good Luck! Christine Tambasco, HT (ASCP) ST. Mary's Hospital 427 Guy Park Ave Amsterdam, New York 12010 >From: "Sue Barnes" >To: >Subject: [Histonet] formula 83 >Date: Mon, 11 Apr 2005 14:58:49 -0400 > >Hi, >Has anyone had any experience with the formula 83 replacement for xylene? > >Received a flyer on it and was wondering if anyone was using it yet. > >Sue Barnes CT,HT (ASCP) > >sbarnes@elch.org >East Liverpool City Hospital >East Liverpool, Ohio > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 12 Apr 2005 00:40:02 +0000 From: "Christine Tambasco" Subject: Re: [Histonet] microtome question To: Vickroy.Jim@mhsil.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format="flowed" maybe it's the cassettes that are not fitting right? It's possible to get a bad batch. Try it and good luck! Christine Tambasco, HT (ASCP) St. Mary's Hospital @ Amsterdam, New York >From: RCHIOVETTI@aol.com >To: Vickroy.Jim@mhsil.com, histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] microtome question >Date: Fri, 8 Apr 2005 16:25:21 EDT > >In a message dated 4/8/2005 1:07:44 PM US Mountain Standard Time, >Vickroy.Jim@mhsil.com writes: > > > we are having > > some problems with the block holders not holding the tissue firm enough > > and therefore the blocks can move and therefore chunk. We have tried a > > couple of new holders but are experiencing the same problem. > >Jim, > >You may already have ruled this out, but I wonder if the problem could be in >the knife holder instead of the cassette clamp? It's strange that you get the >same problem with more than one new specimen holder...nless there was some >kind of manufacturing defect that went undetected, I guess. > >My Two Cents' Worth! > >Cheers, > >Bob > >Robert (Bob) Chiovetti, Ph.D. >Independent Consultant for The Science, Technology and Industrial Sectors >132 North Elster Drive >Tucson, AZ 85710-3212 USA >Tel./Fax 520-546-4986 >Member, Arizona Small Business Association - ASBA >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 11 Apr 2005 18:59:37 -0700 (PDT) From: Jacqueline Lair Subject: Re: [Histonet] microtome question To: histonet@lists.utsouthwestern.edu Message-ID: <20050412015938.34862.qmail@web81509.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii I was having a similar problem with the microtomes in our lab.. the block would chunk out the tissue and the block seemed as if it were barely held into the block holder, very "wobbly like". You could wiggle the block around in the block holder after it was clamped in. This is what I did: Look at the block holder as its held or in the "open" position you would need to have it to insert a block. You should see one or two small openings (the "openings" will either be on the bottom or on the sides of the block holder, depending on which type of microtome you are using.) between the metal on the block holder; i.e. where the metal would come or clamp together to hold a paraffin block. Next, take a pick and scrape the paraffin out of the "opening(s)" in the block holder. When I did the scraping, my block holder had a bunch of paraffin built up "inside" of it, which was causing the block holder to not clamp down as tightly or securely on the block. I find that if I face in many blocks at one time, I have to scrape out the block holder after Ive finished all of my facing. Now, Ive just gotten into the habit of scraping out the block holder after Im done facing to ensure that there is no paraffin build up within the block holder at all. I hope this makes sense? I hope it helps. Jacqueline Lair, HT (ASCP) ------------------------------ Message: 12 Date: Tue, 12 Apr 2005 17:25:56 +1200 From: "Kerry Patience" Subject: [Histonet] Formula 83 To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Is formula 83 a xylene substitute? What company produces it and how does the price compare to xylene? ------------------------------ Message: 13 Date: Tue, 12 Apr 2005 09:29:02 +0100 From: "Burns, Julie" Subject: [Histonet] Delaware diamond knives To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi I work in a busy electron microscopy unit and I am considering the purchase of a delaware diamond knive - I was wanting to know of anyones experiences of these diamond knives ? many thanks Julie Burns ------------------------------ Message: 14 Date: Tue, 12 Apr 2005 15:13:35 +0200 From: "Jens Lindegaard" Subject: [Histonet] Porcine macrophages To: histonet@lists.utsouthwestern.edu Message-ID: Hello Can anyone tell me which antibody to use in order to immunostain porcine macrophages in paraffin embedded sections? Jens Lindegaard M.D. Eye Pathology Institute Frederik V's Vej 11, 1.floor DK-2100 Copenhagen Denmark Phone +45 3532 6083 Fax +45 3532 6080 ------------------------------ Message: 15 Date: Tue, 12 Apr 2005 14:59:39 +0100 From: "Prada-Puentes Carlos \(RW6\) PAHNT" Subject: [Histonet] WT1 To: "HISTONET" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi there, I have been using WT1 with good results from Santa Cruz biotechnology. My problem is that after 6 months the antibody has become unstable and altough we have concentrated it the nuclear staining is weaker and weaker. The datasheet does not recommend freezing and gives ONLY ONE YEAR!!!! Of guaranteed stability kept at 4 C..Should I freeze it when the new vial comes? Considering we use at a dilution of 1/3000 we need to freeze before we use it all. Thank you to all Dr Carlos Prada-Puentes ------------------------------ Message: 16 Date: Tue, 12 Apr 2005 07:01:15 -0700 (PDT) From: Histology SLU Subject: [Histonet] Known Gastrin Controls To: histonet@lists.utsouthwestern.edu Message-ID: <20050412140115.83247.qmail@web51007.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Hello All: Does anyone have any extra human gastrin IHC control blocks that you can spare? Thanks in advance for your help and have a wonderful day! Susan --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! ------------------------------ Message: 17 Date: Tue, 12 Apr 2005 10:01:14 -0500 From: "Kapoor, Sue" Subject: [Histonet] vendors To: histonet@lists.utsouthwestern.edu Message-ID: <61E9F2400F53D5119CFC00508B44E33B02E9E0D1@khmcexch.uhsi.org> Content-Type: text/plain; charset=iso-8859-1 To all vendors, I'm looking for a used fume hood for a Tissue-Tek DRS-601 slide stainer and a used Tissue-Tek Cryo Console. Please email me at: sue.kapoor@uhsi.org Thank you, Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 ------------------------------ Message: 18 Date: Tue, 12 Apr 2005 09:46:35 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] Porcine macrophages To: "'Jens Lindegaard'" , Message-ID: <200504121546.j3CFkQOD004555@chip.viawest.net> Content-Type: text/plain; charset="US-ASCII" I use RDI-Macropabm-387 (Mac387) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jens Lindegaard Sent: Tuesday, April 12, 2005 6:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Porcine macrophages Hello Can anyone tell me which antibody to use in order to immunostain porcine macrophages in paraffin embedded sections? Jens Lindegaard M.D. Eye Pathology Institute Frederik V's Vej 11, 1.floor DK-2100 Copenhagen Denmark Phone +45 3532 6083 Fax +45 3532 6080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Tue, 12 Apr 2005 11:49:02 -0400 From: meyenhmf@umdnj.edu Subject: Re: [Histonet] vendors To: "Kapoor, Sue" Cc: histonet@lists.utsouthwestern.edu Message-ID: <1113320942.425bedeee6f1a@webmail.umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1 I have a slightly used (1 year in a small hospital) ^)! with hood available. Also lots of other EM, Histo and Lab Equipment. Please contact me at: micro@superlink.net Regards, Markus F. Meyenhofer Microscopy Labs micro@superlink.net Quoting "Kapoor, Sue" : > To all vendors, I'm looking for a used fume hood for a Tissue-Tek > DRS-601 > slide stainer and a used Tissue-Tek Cryo Console. > > Please email me at: > sue.kapoor@uhsi.org > > Thank you, > Sue Kapoor, HT (ASCP) > Histology Coordinator > Kenosha Medical Center > Kenosha, WI > 262-653-5570 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- ------------------------------ Message: 20 Date: Tue, 12 Apr 2005 10:55:04 -0500 From: "Rush, Joyce" Subject: [Histonet] Recycling To: Message-ID: <2337362E8548BE4C85EE5DD5D526B0851942B4@sjw3smail2.SJMCMN.ORG> Content-Type: text/plain; charset="iso-8859-1" We are looking into recycling our alcohol, formalin and Americlear. I'd love to hear any experiences doing this. We are a relatively small user and currently sewer everything. I'm trying to gather information for the day when we can not do this. Thanks, Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 ------------------------------ Message: 21 Date: Tue, 12 Apr 2005 10:11:10 -0600 From: "Patsy Ruegg" Subject: [Histonet] Tape transfer sections and IHC To: Message-ID: <200504121611.j3CGB1OD014811@chip.viawest.net> Content-Type: text/plain; charset="US-ASCII" I am doing hrp IHC on tape transfer sections and I am experiencing a problem with unwanted staining on the coating on the polymer coated slides. Does anyone else out there do IHC on the tape sections experience this problem? Please suggest a solution to this. Thank you, Patsy ------------------------------ Message: 22 Date: Tue, 12 Apr 2005 11:18:39 -0500 From: "Connie Sims" Subject: RE: [Histonet] Recycling To: histonet@lists.utsouthwestern.edu Message-ID: <11906C81B7B03E47A6F10CE5F4784449018A9C1F@dchexch01.driscoll.dch> Content-Type: text/plain; charset=us-ascii We currently have a CBG recycler. I love it! It does take some tech time and planning but the cost savings and environmental benefits are worth it. Formalin requires rebuffering and alcohol is only 95%. Americlear is completely reuseable. I haven't had any problems so far, its been almost a year now. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rush, Joyce Sent: Tuesday, April 12, 2005 10:55 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling We are looking into recycling our alcohol, formalin and Americlear. I'd love to hear any experiences doing this. We are a relatively small user and currently sewer everything. I'm trying to gather information for the day when we can not do this. Thanks, Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================================== ================================ Driscoll Children's Hospital Disclaimer: This e-mail message and all information enclosed, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution of the information enclosed, in its entirety or in part, is strictly prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and immediately destroy all copies of the message. For further assistance or questions please contact the email administrator at Driscoll Children's Hospital at (361) 694-5000. ======================================================================== ================================= ------------------------------ Message: 23 Date: Tue, 12 Apr 2005 12:52:37 -0400 From: "Favara, Cynthia (NIH/NIAID)" Subject: RE: [Histonet] Porcine macrophages To: 'Patsy Ruegg' , 'Jens Lindegaard' , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain Have you looked into F4/80? c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Tuesday, April 12, 2005 8:47 AM To: 'Jens Lindegaard'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Porcine macrophages I use RDI-Macropabm-387 (Mac387) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jens Lindegaard Sent: Tuesday, April 12, 2005 6:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Porcine macrophages Hello Can anyone tell me which antibody to use in order to immunostain porcine macrophages in paraffin embedded sections? Jens Lindegaard M.D. Eye Pathology Institute Frederik V's Vej 11, 1.floor DK-2100 Copenhagen Denmark Phone +45 3532 6083 Fax +45 3532 6080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 17, Issue 20 **************************************** From jdmd77 <@t> hotmail.com Wed Apr 13 10:13:34 2005 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Isopropyl (again) In-Reply-To: <20050413140642.59674.qmail@web30403.mail.mud.yahoo.com> Message-ID: A technique that I have found useful in assisting in decreasing fixation time for breast tissue: (1) Write the patient's last name and accession number on a paper towel (or 3) (2) If the breast specimen is received oriented, write MEDIAL or LATERAL or whatever "landmark you do KNOW on one edge of the paper towel (3) As soon as possible after the fresh specimen is received, ink the margins of the breast tissue and apply mordant (acetic acid or Bouins) (4) section the fresh, inked tissue as thinly as possible (usually between 4 and 8 mm) (5) As each section is cut from the gross specimen, "stretch" the tissue out on the labeled DRY paper towel, allowing expansion of the tissue, maintaining the integrity of the orientation (i.e. gross serial sections placed next to one another (6) FOLD the paper towel(s) over the fresh breast tissue and place the SECTIONED, oriented breast tissue in at least 5 times volume (but preferably 10X volume) fixative. This method allows a greater surface area of the breast tissue to be fixed simultaneously than the general method of leaving a lump of fatty breast tissue in fixative overnight. Remember that formalin fixes at approximately 1 mm per hour. If you have a 6 mm sections of breast tissue handled in this manner - the tissue needs to be in fixative for 6 hours PRIOR to cassetting the representative sections. Remind surgeons that their patients are best served when these "STAT" cases are done in the morning - and I agree with Dr. Peters that routine education of our clinical colleagues is helpful in promoting the "we're on the same team" for each patient environment. Just my opinion, Julia >From: "Stephen Peters M.D." >To: Histonet@lists.utsouthwestern.edu >Subject: re:[Histonet] Isopropyl (again) >Date: Wed, 13 Apr 2005 07:06:41 -0700 (PDT) >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by mc4-f8.hotmail.com >with Microsoft SMTPSVC(6.0.3790.211); Wed, 13 Apr 2005 07:08:16 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1DLiWF-000336-Pz; Wed, 13 Apr >2005 09:07:08 -0500 >Received: from [199.165.152.167] (helo=swlx167.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1DLiVu-00032o-JTfor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 09:06:52 -0500 >Received: from [68.142.200.106] (helo=web30403.mail.mud.yahoo.com)by >swlx167.swmed.edu with smtp (Exim 4.44) id 1DLiVu-0001X6-5sfor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 09:06:42 -0500 >Received: (qmail 59676 invoked by uid 60001); 13 Apr 2005 14:06:42 -0000 >Received: from [69.74.36.202] by web30403.mail.mud.yahoo.com via HTTP;Wed, >13 Apr 2005 07:06:41 PDT >X-Message-Info: cmh8WMAfDXrY5PGm8SFuqC+zJHouuuoQgEcbCHoYkiw= >Comment: DomainKeys? See http://antispam.yahoo.com/domainkeys >DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=s1024; >d=yahoo.com;b=r52rWHDCB3Q4M5y8kSQJUACbg0eHfeql5y16JxAMnxRtIGNiCQRtpHhV6SDfzR/YbMvqJW1fR4QYlXUCV7BBeIjv3b6Gc8lkbujfBrVh/juAR9mVLMG+NsPsoq8gNyolHOgwbFSulaIyEB572h19CqOGjUXs6l+XeV/4R06Brag=; >X-Scan-Signature: a3aea070cbb59d170c13cfd6234bf193 >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 280efbe157041e2ff2738647b8dc565c >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.5 required=5.5 >tests=FORGED_YAHOO_RCVD autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 13 Apr 2005 14:08:16.0701 (UTC) >FILETIME=[3D113ED0:01C54032] > >I cannot help you with the isopropal question. But let me ask you are you >trying to speed >up your turn around time on breasts or are you having trouble cutting under >processed >tissue? > If you are trying to speed up turn around time you picked a bad tissue to >be in a >rush with! Reading a miserably processed breast specimen is a frustrating >and risky >buisness frought with potential legal repercussions. I think your >pathologists would > agree with this. I suggest a little patience. If the surgeons are busting >their chops it is time >for a little histology lesson for the surgeons. I like to tell my surgeons >I will be be happy to give a rush diagnosis, but if they would like the >correct diagnosis that will take a bit longer! > >If poorly processed tissue is the problem then it is a matter of all the >things you already know. The sections should be cut no more than 3mm thick >across the entire section > ( not just the edge). Once cut sections should be give adequate fixation >in clean >formalin before putting it in the processor. Over night if possible. >Processor solutions should >be clean and changed at appropriate intervals. Baskets should not be >overstuffed if possible especially with, cassettes filled with large >samples. If you have multiple processors at your disposal you may want to >sequester these cases an adjust the program and solution >changes until you are satisfied with the result. >If your grossing staff leaves intact specimen sit overnight in the >fixative it arrived in and >then cuts it the next day, fixation will often not be adequate because >inadequate amout > of formalin, formalin which has been weakened by dilution or inability of >the formalin >to penetrate and fix a thick piece of tissue. > >I am sure you already know this . Make sure your grossing staff do to. > > > >Stephen Peters M.D. >Vice Chairman of Pathology >Hackensack University Medical Center >201 996 4836 > >Pathology Innovations, LLC >410 Old Mill Lane, >Wyckoff, NJ 07481 >201 847 7600 >www.pathologyinnovations.com > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Apr 13 10:21:40 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] RE: controls and secondaries Message-ID: <6.0.0.22.1.20050413084637.01b222e0@gemini.msu.montana.edu> What is the difference between a secondary such as a goat anti- mouse and a straight mouse IgG istotype control as far as it's applications in the IHC process? *****The IgG isotype is used as the negative control, and must match the concentration of your primary. This is preferred to using normal mouse serum as a negative control since serums can contain nonspecific antibodies which can cause nonspecific background. I used normal serum one time and publication reviewers balked big time, I had to go back and redo ( a VERY difficult experiment) ALL my immunostaining using a mouse IgG isotype control. Haven't use normal serums of any sort since that time. So to set up your IFA or even IHC staining, you would have a primary (mouse anti-whatever) on one section and mouse IgG (it may be IgG1, IgG2a, etc, etc) at same concentration your primary on a separate section labeled negative control. at tI ask because someone from another lab recently came to me for help with a long list of stains they need done in a short period of time. They have already ordered everything they think they need to complete the stains. Most of them are flouescent conjugated primaries, but two will have to have the fluorescence attached to the secondary. *****Extra comment here: If the directly conjugated primary mouse antiwhatever-FITC fails to work, you can use DAKO's rabbit antiFITC, then come back with goat antirabbbit-FITC OR Donkey antiRAbbit-FITC. I have had primaries that work in FACS that will NOT work i.e. fluoresce on tissue sections. It is a spatial, stoichiometry (sp?) problem, but easily solved in a rather simple way, an extra two steps. Do not buy DAKO's antirabbit conjugated to anything, it doesn't work as well as I described. I hope this does not happen to you. **** IF your primary is conjugated to FITC or any other fluorophore, then the mouse IgG isotype must be conjugated to FITC also in order to have a correct negative control. If you have a problem with this, you can get mouse IgG (which contain ALL the isotypes in one whole IgG) conjugated to FITC from Jackson (fast, cheap and excellent) - still legal to use as long as the isotype is there. I am wondering about this because I was asked why their protocol would call specifically for goat serum to block, and would it matter if it was another species. *****As long as they are using goat antimouse, then the blocking serum is goat. This is what we do here. Blocking serum, even though you are using a directly FITC conjugated mouse antiwhatever could be goat, that is not uncommon. Swine, in your case would be avoided, but some use donkey, horse but we prefer to do what you said in next statement. I told them that usually the blocking serum is the same as the species the secondary was made in and was told that the secondary wasn't made in anything different such as a goat anti-mouse, they had what I would normally use as control serum. ???Question here: are you saying your control serum is mouse serum and used as the negative control? As said in beginnign, can contain nonspecific antibodies that could cause background as I said in beginning. *****If you are using a goat antimouse, the blocking serum should be goat serum. It can be used as or in the diluent for the goat antimouse-FITC secondary also. With FITC conjugated secondaries, we would use approx 2 - 5% goat serum in diluent and no BSA. If you were using donkey antimouse, then the blocking serum becomes donkey serum. A word to the wise when working with large animals, it is a good idea to have purchased a seondary adsorbed to the species being stained. In your case that would be Goat antiMouse-FITC adsorbed to swine. Can it be used as a secondary? I have never tried this. Supposedly it worked with their flow samples as a secondary (these are mouse primaries on pig tissue). ****I got lost here, you ARE using a goat antimouse (conjugated to FITC?)to detect your mouse antiwhatever on pig tissue section, correct? And that is what they used in FACS analysis too? If you wish, I will be happy to discuss this further privately. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From sfarley <@t> seattlecca.org Wed Apr 13 10:32:46 2005 From: sfarley <@t> seattlecca.org (Farley, Sunni R) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Methyl Green-Pyronin staining for nucleic acids Message-ID: <5E6BFDF4F0AB2C4DA69CF4473FC7B94832330F@wala01.seattlecca.org> I am looking for information regarding Methyl Green-Pyronin staining for nucleic acids. I recently contacted Rowley Biochemical Inc (Danvers, MA) about their Methyl Green Pyronin stain and Differentiating Solution. They provided me with the chemical composition of these solutions and also the protocol for Taft's Method for Nucleic Acids. Does anybody routinely use these items and this method? Also, have you used them on B5-fixed tissue? Any information and/or suggestions would be greatly appreciated. Thank you for your time! Sunni R. Farley Histotechnician Clinical Pathology Mailstop G1-201 Seattle Cancer Care Alliance 825 Eastlake Ave East Seattle, WA 98109 (206) 288-1312 This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From katri <@t> cogeco.ca Wed Apr 13 10:37:45 2005 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Isopropyl (again) References: Message-ID: <001a01c5403e$bd0be2f0$6a9a9618@Katri> Also, remember, if Her2 is requested later on, Dako's Her2 Kit recommends minimum 24 hour fixation in NBF for results to be valid and reliable. I understand in US, in order for the patient to be treated and reembursed by insurance companies, Dako Kit must be used. Patient comes first, not the TAT. My two cents worth... Katri Katri Tuomala Hamilton, Ontario, Canada From Lawrence.Brett <@t> luht.scot.nhs.uk Wed Apr 13 04:38:45 2005 From: Lawrence.Brett <@t> luht.scot.nhs.uk (Brett, Lawrence) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] tissue softening agent Message-ID: <857344E7D8F90240935A288998A89D811C60B0@wgh-ex1.luht.scot.nhs.uk> Pam This is common fabric conditioner in the UK, produced by Unilever. Available from most grocery shops over here, check out the website http://www.unilever.co.uk/ourbrands/homecare/comfort.asp or phone their customer careline on (UK) 0800 776647. Never heard of it being used in histology though! Lawrence Brett Edinburgh, UK -----Original Message----- From: Pam O'Neill [mailto:onep00@hotmail.com] Sent: 11 April 2005 04:52 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue softening agent Anyone with experience in paleo pathology----- I am looking for information on a softening agent from England called "comfort". It is used in the textile industry, but supposedly is good for softening dessicated tissue. A company name and a phone number would be very helpful. Thankyou, Pam O'Neill SVMMC Toledo, Ohio _________________________________________________________________ Don't just search. Find. Check out the new MSN Search! http://search.msn.click-url.com/go/onm00200636ave/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** The information contained in this message may be confidential or legally privileged and is intended for the addressee only, If you have received this message in error or there are any problems please notify the originator immediately. The unauthorised use, disclosure, copying or alteration of this message is strictly forbidden. ********************************************************************** From rgrow <@t> bmnet.com Wed Apr 13 11:11:28 2005 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Re:Microscope Service In-Reply-To: <200504131438.j3DEc0Jc019095@dns1.bmnet.com> Message-ID: Contact Appalachian Microscope Service: 865-983-2594 for repair and maintenance of your microscopes. They are located in Alcoa, TN and have serviced our scopes for years. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax Message: 21 Date: Wed, 13 Apr 2005 09:48:30 -0400 From: dndsomi@aol.com Subject: [Histonet] Microscope Service To: histonet@lists.utsouthwestern.edu Message-ID: <8C70E469C9E2B5D-9E0-26B71@mblk-r21.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" We are trying to find a microscope service person in the East Tennessee area. Does anyone have contact information? Thanks. DDietz From RRA <@t> Stowers-Institute.org Wed Apr 13 11:16:14 2005 From: RRA <@t> Stowers-Institute.org (Allen, Rhonda) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Serotonin on Mouse Message-ID: Hello, Is anyone using Serotonin on mouse embryos successfully? If so, what company and protocol are you using? Rhonda Allen Stowers Institute 1000 E. 50th street Kansas City, Missouri 64110 rra@stowers-institute.org From rgrow <@t> bmnet.com Wed Apr 13 11:16:17 2005 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Re: Cost of automated immunos? In-Reply-To: <200504131438.j3DEc0Jc019095@dns1.bmnet.com> Message-ID: I vote for Dako, too Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ------------------------------ Message: 23 Date: Wed, 13 Apr 2005 10:13:12 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Cost of automated immunos? To: "Sebree Linda A." , , Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA45947@sjhaexc02.sjha.org> Content-Type: text/plain; charset="iso-8859-1" Also, DAKO. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sebree Linda A. Sent: Wednesday, April 13, 2005 8:49 AM To: Krat18@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cost of automated immunos? Chris, I suggest contacting Ventana, they can give you a pretty accurate cost estimate per slide. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Krat18@aol.com Sent: Wednesday, April 13, 2005 3:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost of automated immunos? Our lab is starting to think about doing automated immunos. Can anyone out there is Histoland give me an idea of the COST of doing an automated immuno vs. a manual one? I know there are many variables, but we're looking at the Benchmark, so any idea of the cost per test on that machine? We would appreciate any input. Thanks in advance! Chris Davis _chrisdavis@hotmail.com_ (mailto:chrisdavis@hotmail.com) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. ------------------------------ Message: 24 Date: Wed, 13 Apr 2005 07:17:47 -0700 (PDT) From: Histology SLU Subject: RE: [Histonet] Cost of automated immunos? To: "Weems, Joyce" , "Sebree Linda A." , Krat18@aol.com, histonet@lists.utsouthwestern.edu Message-ID: <20050413141747.63770.qmail@web51006.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii I second DAKO. "Weems, Joyce" wrote:Also, DAKO. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sebree Linda A. Sent: Wednesday, April 13, 2005 8:49 AM To: Krat18@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cost of automated immunos? Chris, I suggest contacting Ventana, they can give you a pretty accurate cost estimate per slide. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Krat18@aol.com Sent: Wednesday, April 13, 2005 3:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost of automated immunos? Our lab is starting to think about doing automated immunos. Can anyone out there is Histoland give me an idea of the COST of doing an automated immuno vs. a manual one? I know there are many variables, but we're looking at the Benchmark, so any idea of the cost per test on that machine? We would appreciate any input. Thanks in advance! Chris Davis _chrisdavis@hotmail.com_ (mailto:chrisdavis@hotmail.com) From peoshel <@t> wisc.edu Wed Apr 13 11:26:11 2005 From: peoshel <@t> wisc.edu (Philip Oshel) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Eudora and no email messages In-Reply-To: <6.0.0.22.1.20050413084021.01b12a30@gemini.msu.montana.edu> References: <6.0.0.22.1.20050413084021.01b12a30@gemini.msu.montana.edu> Message-ID: Gayle, I'm using Eudora 5.2 in the lab and 6.1 at home, and -- sorry -- don't have any trouble receiving histonet. This is with Macs, OS 9.2.2. Phil >Is something wrong with the system? I have received no emails via >Eudora since late yesterday afternoon. Nothing comes in when I open >the "Check Mail" box. > >Extension is 6367 >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 (lab with voice mail) >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) From froyer <@t> bitstream.net Wed Apr 13 11:51:43 2005 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Re:Microscope Service In-Reply-To: References: Message-ID: <425D4E1F.3040501@bitstream.net> Another source close to TN: Lab Optical Services 20222 Avondale Rd Abingdon, VA 24210-6942 Attn.: Bill Hatcher (276) 628-4120 ~ Ford Ford M. Royer Midwest Science Biocenter Minneapolis, MN rgrow@bmnet.com wrote: >Contact Appalachian Microscope Service: 865-983-2594 for repair and >maintenance of your microscopes. They are located in Alcoa, TN and have >serviced our scopes for years. > >Renee Grow, BA., HT (ASCP) >rgrow@bmnet.com >Histology Supervisor >Blount Memorial Hospital >907 E. Lamar Alexander Pkwy. >Maryville, TN 37804-5016 >(865) 977-4744 >(865) 977-5766 Fax > >Message: 21 >Date: Wed, 13 Apr 2005 09:48:30 -0400 >From: dndsomi@aol.com >Subject: [Histonet] Microscope Service >To: histonet@lists.utsouthwestern.edu >Message-ID: <8C70E469C9E2B5D-9E0-26B71@mblk-r21.sysops.aol.com> >Content-Type: text/plain; charset="us-ascii" > >We are trying to find a microscope service person in the East Tennessee >area. Does anyone have contact information? Thanks. >DDietz > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From gcallis <@t> montana.edu Wed Apr 13 12:11:30 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Sorry, message was meant for ITC about Eudora and no email messages In-Reply-To: References: <6.0.0.22.1.20050413084021.01b12a30@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20050413105653.01b3c700@gemini.msu.montana.edu> Dear ALL, Sorry folks, but I messed up a filter setup on Eudora, now rectified. All Histonet and other email went to trash, felt lost in cyberspace. The original message about not getting emails was meant for our university ITC, ended up on Histonet too. Eudora is working fine as long as I am tweaking things willy nilly. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Wed Apr 13 12:36:06 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Help Are Histonetters getting kickback messages Message-ID: <6.0.0.22.1.20050413112255.01b86618@gemini.msu.montana.edu> Dear All - a major gripe I have been plagued by a constantly reoccuring kickback message from some stupid mail administrator at National Blood Services in UK. If the woman who is supposed to be getting Histonet (maybe not) would unsubscribe, I would be sooo happy, and this would come to a halt. If anyone knows this person in UK at this email address: sarah.white@nbs.nhs.uk - please tell them to fix it!! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From slappycraw <@t> yahoo.com Wed Apr 13 12:41:55 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Help Are Histonetters getting kickbacks Message-ID: <20050413174155.54188.qmail@web52603.mail.yahoo.com> How much are the kickbacks? --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From Joyce.Rush <@t> sjmcmn.org Wed Apr 13 12:57:07 2005 From: Joyce.Rush <@t> sjmcmn.org (Rush, Joyce) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Long and short biopsy runs with one processor Message-ID: <2337362E8548BE4C85EE5DD5D526B0851942C0@sjw3smail2.SJMCMN.ORG> We are a community hospital and last year processed 28,000 blocks. We are being challenged by our Laboratory Director to run a long and short, biopsy run daily, M-F. Currently we run everything together. We have one processor, a VIP5. I'd love to hear of ways people have handled this. Thanks so much! I always get such good advice from this group! Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 From Charlene.Henry <@t> STJUDE.ORG Wed Apr 13 13:06:01 2005 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Hepatitis C Probe Message-ID: <5CB39BCA5724F349BCB748675C6CA1A202C756CA@SJMEMXMB02.stjude.sjcrh.local> Hello Histonet, Does anyone know of a good Hepatitis C Probe? We are wanting to add the Hepatits C to our list of viral ISH protocols. Thanks, Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From jdmd77 <@t> hotmail.com Wed Apr 13 13:12:38 2005 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Long and short biopsy runs with one processor In-Reply-To: <2337362E8548BE4C85EE5DD5D526B0851942C0@sjw3smail2.SJMCMN.ORG> Message-ID: Joyce - First let me preface with my bias - I am a GI pathologist (read small biopsy material is what I live and breathe). Long processor runs are great for standard surgical material - but absolutely overprocess small pieces of tissue, resulting in hard, dehydrated, difficult to cut little brittle fragments. The best approach that I've seen and used is to examine your "bottlenecks." The main bottlenecks are the points at which you have lots to do in front of you - with limited resource to do it (i.e. you have 150 cassettes to embed coming off of the processor at one time and ONE embedding station. That's a bottleneck.) What time do you usually start your processor? Say your processor starts at 10:00 p.m. with a standard 6 hour process run. Your pathologists or your PAs close the grossing stations at 6:00 p.m. and load the processor - and everything sits there for 4 hours (that's another bottleneck). In this scenario - you can add a second processor run at virtually any time that you are staffed that allows you to turn over the processor for the standard run by 6:00 p.m. (when the standard next load is). Since a small biopsy run usually takes about 2 hours on the Vitek: 0600 - Standard processor emptied - large surgical cases ONLY (about 2/3 of your blocks) 0600 - 1000: Embed, cut, stain and coverslip surgicals 0700 - 0800: Previous day's and early AM courier run of small biopsies grossed in 0800 - 1000: Small biopsy processor run 1000 - small biopsy processor emptied 1000 - 1230: Embed, cut, stain and coverslip biopsies. If you wanted to do another biopsy run - to allow same day turnaround time - the 2nd biopsy run could be scheduled at 10:30 each day with slides coming out around 3:30. I'm interested to hear other people's ideas. Julia >From: "Rush, Joyce" >To: >Subject: [Histonet] Long and short biopsy runs with one processor >Date: Wed, 13 Apr 2005 12:57:07 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by mc7-f23.hotmail.com >with Microsoft SMTPSVC(6.0.3790.211); Wed, 13 Apr 2005 10:58:11 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1DLm7H-0004v5-7P; Wed, 13 Apr >2005 12:57:36 -0500 >Received: from [199.165.152.166] (helo=swlx166.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1DLm6v-0004uy-Kqfor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 12:57:19 -0500 >Received: from [66.173.44.122] (helo=keys.sjmcmn.org)by swlx166.swmed.edu >with esmtp (Exim 4.44) id 1DLm6u-0004b0-5Ffor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 12:57:09 -0500 >Received: from keys.sjmcmn.org (unknown [127.0.0.1])by keys.sjmcmn.org >(Postfix) with ESMTP id 50777256FE9for >;Wed, 13 Apr 2005 12:57:07 -0500 (CDT) >Received: from mail.SJMCMN.ORG ([10.1.0.6])by keys.sjmcmn.org (PGP >Universal service);Wed, 13 Apr 2005 12:57:07 -0500 >X-Message-Info: cmh8WMAfDXqeqGy5Qdhsq/iJQn/CwXc1jm673ZQpEo0= >X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 >Content-class: urn:content-classes:message >X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: Long and short biopsy >runs with one processor >Thread-Index: AcVAUjUG93SXzh8HQoGO/FSJK5JRgA== >X-Scan-Signature: 5ce762e8aa474ea7aaa37532c083de4e >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 201832d891fa504721b2e650a5a52212 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 13 Apr 2005 17:58:11.0794 (UTC) >FILETIME=[5B956320:01C54052] > >We are a community hospital and last year processed 28,000 blocks. We are >being challenged by our Laboratory Director to run a long and short, biopsy >run daily, M-F. Currently we run everything together. We have one >processor, a VIP5. I'd love to hear of ways people have handled this. >Thanks so much! I always get such good advice from this group! > >Joyce > >Joyce A. Rush, BS, MT(ASCP) >Laboratory Manager >St Joseph's Medical Center >523 North Third Street >Brainerd, MN 56401 >Office:218-828-7500 Fax: 218-828-7510 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Wed Apr 13 13:54:34 2005 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] varistain 24-3 instruction manual Message-ID: <3E5A3F039F0BD8118B4700C00D00202404354B@CKHA9> Does anyone have an instruction manual for a Shandon Varistain 24-3 autostainer? If the main pages could be scanned and emailed, it would be appreciated. diana From cjohnston <@t> mdanderson.org Wed Apr 13 14:09:01 2005 From: cjohnston <@t> mdanderson.org (cjohnston@mdanderson.org) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Tampa Area Message-ID: I am looking for anyone familiar with Sun Coast Hospital in the Tampa area. If you could email me privately, I would appreciate it . Carol Johnston Carol M. Johnston HT(ASCP) Division of Surgery M.D. Anderson Cancer Center 1515 Holcombe Blvd. Unit 017 Houston, Texas 77030 713-745-4625 fax: 713-745-2548 From VBlanc <@t> asterand.com Wed Apr 13 14:28:28 2005 From: VBlanc <@t> asterand.com (Vici Blanc) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Histotechnician position at Asterand Message-ID: <56E63440ABF1FC4897947D5BA4CFA44F04CD15E1@ATL1VEXC005.usdom004.tco.tc> Dear Histonetters, Asterand, a Detroit-based commercial tissue bank is looking for a histotechnician. The job description follows. If interested, please submit your resume to Christine Milne, human resources manager (cmilne@asterand.com), or you may reply to this email and I will forward it on to her. Thank you, Victoria M. Blanc, Ph.D. Director of Research Services Asterand, Inc 440 Burroughs TechOne, suite 501 Detroit, MI 48202 Phone: 313-263-0960, ext. 240 Fax: 313-263-0961 www.asterand.com POSITION SUMMARY: Prepares tissue specimens for routine and special procedures to confirm tissue diagnosis. Performs complex histological procedures, records and analyzes data, maintains and repairs instruments. Relies on experience and judgment to pan and accomplish goals. Works under general supervision. POSITION DUTIES AND RESPONSIBILTIES: * Slide preparation for Pathology review * Section slides from both paraffin embedded and OCT embedded tissues * Cryosectioning, Microtome sectioning and tissue staining and cover slipping * Data entry and tracking of all products derived from sectioning * General laboratory and equipment maintenance * Potential TMA construction * Custom sectioning for sales orders * Other duties as assigned. MINIMUM EXPERIENCE AND/OR EDUCATION REQUIRED: * Certification for Histotechnician HT * Requires Associate's degree or its equivalent * 2 to 4 years of experience in the field or related area PERSONAL ATTRIBUTES: * Excellent written and verbal communication skills * Interpersonal skills * Organizational skills and attention to detail * Problem solving skills * Passion for excellence PHYSCIAL DEMANDS: Standing for long periods of time This e-mail message, together with any attachments, contains information belonging to Asterand, Inc. that may be confidential, proprietary, copyrighted and/or legally protected or privileged, and is intended for the sole use of the individual or entity named on this message. If you are not the intended recipient, and have received this e-mail message in error, please immediately return this message to its original sender and permanently delete it from your electronic and paper records. Thank You. From aetechserv01 <@t> yahoo.com Wed Apr 13 16:29:59 2005 From: aetechserv01 <@t> yahoo.com (dfgder dsfvdfg) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Limited background Message-ID: <20050413212959.2689.qmail@web61208.mail.yahoo.com> Fellow Histonetters! I am having problems with background in connective tissue - specifically, sharpey's fibers from jaw bone. Has anyone encountered this before ? The dilution is OK. Other causes? Thanks AE __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From bills <@t> icpmr.wsahs.nsw.gov.au Wed Apr 13 16:30:48 2005 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Long and short biopsy runs with one processor In-Reply-To: Message-ID: <001701c54070$1002c920$0ecd080a@wsahs.nsw.gov.au> Julia, I agree with your philosophy about small runs. We are a pathology department supporting a large teaching hospital and an even larger area pathology service. However, we also do a considerable amount of private work for several endoscopy clinics in our locality. The scenario you describe is very similar to ours. We have the usual overnight run, usually 450-600 blocks with the large material in one processor and the smaller in another. We receive the endoscopy specimens anytime from 7:30am through to 3:00pm each day, with any pick ups after 6:00pm being processed the next morning in a short run 2-2.5hrs. This means we can have at least two runs per day of endoscopy specimens. The endoscopy specimens from the private clinics are about 30% of our work and the TAT for these specimens is >2 days from pick-up to hard copy result to the clinician. We also process renal biopsies several times per day as well as the occasional urgent specimen. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Julia Dahl Sent: Thursday, 14 April 2005 4:13 AM To: Joyce.Rush@sjmcmn.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Long and short biopsy runs with one processor Joyce - First let me preface with my bias - I am a GI pathologist (read small biopsy material is what I live and breathe). Long processor runs are great for standard surgical material - but absolutely overprocess small pieces of tissue, resulting in hard, dehydrated, difficult to cut little brittle fragments. The best approach that I've seen and used is to examine your "bottlenecks." The main bottlenecks are the points at which you have lots to do in front of you - with limited resource to do it (i.e. you have 150 cassettes to embed coming off of the processor at one time and ONE embedding station. That's a bottleneck.) What time do you usually start your processor? Say your processor starts at 10:00 p.m. with a standard 6 hour process run. Your pathologists or your PAs close the grossing stations at 6:00 p.m. and load the processor - and everything sits there for 4 hours (that's another bottleneck). In this scenario - you can add a second processor run at virtually any time that you are staffed that allows you to turn over the processor for the standard run by 6:00 p.m. (when the standard next load is). Since a small biopsy run usually takes about 2 hours on the Vitek: 0600 - Standard processor emptied - large surgical cases ONLY (about 2/3 of your blocks) 0600 - 1000: Embed, cut, stain and coverslip surgicals 0700 - 0800: Previous day's and early AM courier run of small biopsies grossed in 0800 - 1000: Small biopsy processor run 1000 - small biopsy processor emptied 1000 - 1230: Embed, cut, stain and coverslip biopsies. If you wanted to do another biopsy run - to allow same day turnaround time - the 2nd biopsy run could be scheduled at 10:30 each day with slides coming out around 3:30. I'm interested to hear other people's ideas. Julia >From: "Rush, Joyce" >To: >Subject: [Histonet] Long and short biopsy runs with one processor >Date: Wed, 13 Apr 2005 12:57:07 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by mc7-f23.hotmail.com >with Microsoft SMTPSVC(6.0.3790.211); Wed, 13 Apr 2005 10:58:11 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1DLm7H-0004v5-7P; Wed, 13 Apr >2005 12:57:36 -0500 >Received: from [199.165.152.166] (helo=swlx166.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1DLm6v-0004uy-Kqfor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 12:57:19 -0500 >Received: from [66.173.44.122] (helo=keys.sjmcmn.org)by swlx166.swmed.edu >with esmtp (Exim 4.44) id 1DLm6u-0004b0-5Ffor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 12:57:09 -0500 >Received: from keys.sjmcmn.org (unknown [127.0.0.1])by keys.sjmcmn.org >(Postfix) with ESMTP id 50777256FE9for >;Wed, 13 Apr 2005 12:57:07 -0500 (CDT) >Received: from mail.SJMCMN.ORG ([10.1.0.6])by keys.sjmcmn.org (PGP >Universal service);Wed, 13 Apr 2005 12:57:07 -0500 >X-Message-Info: cmh8WMAfDXqeqGy5Qdhsq/iJQn/CwXc1jm673ZQpEo0= >X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 >Content-class: urn:content-classes:message >X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: Long and short biopsy >runs with one processor >Thread-Index: AcVAUjUG93SXzh8HQoGO/FSJK5JRgA== >X-Scan-Signature: 5ce762e8aa474ea7aaa37532c083de4e >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 201832d891fa504721b2e650a5a52212 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 13 Apr 2005 17:58:11.0794 (UTC) >FILETIME=[5B956320:01C54052] > >We are a community hospital and last year processed 28,000 blocks. We are >being challenged by our Laboratory Director to run a long and short, biopsy >run daily, M-F. Currently we run everything together. We have one >processor, a VIP5. I'd love to hear of ways people have handled this. >Thanks so much! I always get such good advice from this group! > >Joyce > >Joyce A. Rush, BS, MT(ASCP) >Laboratory Manager >St Joseph's Medical Center >523 North Third Street >Brainerd, MN 56401 >Office:218-828-7500 Fax: 218-828-7510 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From eileen_dusek <@t> yahoo.com Wed Apr 13 16:49:14 2005 From: eileen_dusek <@t> yahoo.com (eileen dusek) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Clones ER 1D5 and PR 636 Message-ID: <20050413214914.78548.qmail@web30511.mail.mud.yahoo.com> Hi Everyone, Can someone please help me!!!! I need to get these clones working on the Ventana Benchmark. I have tried CC! and CC2, with and without heat, with and without EZ Prep. I am at a loss Thanks for your help. Eileen C. Dusek Edward Hospital __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From bills <@t> icpmr.wsahs.nsw.gov.au Wed Apr 13 16:55:05 2005 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Long and short biopsy runs with one processor In-Reply-To: Message-ID: <000301c54073$74295650$0ecd080a@wsahs.nsw.gov.au> Roxanne, Most are the small GE biopsies >3mm in diamater or "thin" slices of any urgents >3mm thick. Solution Time Formalin 0min 70%ethanol 5min 95% 5min 100% 5min 100% 10min 100% 10min Xylol 5min Xylol 5min Xylol 10min Wax 5min Wax 10min Wax 10min Temperature ambient with Pressur/Vacuum on all stations. Two Leica ASP300 and two VIP 3000 Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: Roxanne Soto [mailto:godsgirlnow@MSN.COM] Sent: Thursday, 14 April 2005 7:37 AM To: Bill Sinai Subject: Re: [Histonet] Long and short biopsy runs with one processor What size is the tissue on the 2 hour run? How long is each station? What kind of processor? Do you use the vacuum? Roxanne ----- Original Message ----- From: Bill Sinai To: histonet (E-mail) Sent: Wednesday, April 13, 2005 5:30 PM Subject: RE: [Histonet] Long and short biopsy runs with one processor Julia, I agree with your philosophy about small runs. We are a pathology department supporting a large teaching hospital and an even larger area pathology service. However, we also do a considerable amount of private work for several endoscopy clinics in our locality. The scenario you describe is very similar to ours. We have the usual overnight run, usually 450-600 blocks with the large material in one processor and the smaller in another. We receive the endoscopy specimens anytime from 7:30am through to 3:00pm each day, with any pick ups after 6:00pm being processed the next morning in a short run 2-2.5hrs. This means we can have at least two runs per day of endoscopy specimens. The endoscopy specimens from the private clinics are about 30% of our work and the TAT for these specimens is >2 days from pick-up to hard copy result to the clinician. We also process renal biopsies several times per day as well as the occasional urgent specimen. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Julia Dahl Sent: Thursday, 14 April 2005 4:13 AM To: Joyce.Rush@sjmcmn.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Long and short biopsy runs with one processor Joyce - First let me preface with my bias - I am a GI pathologist (read small biopsy material is what I live and breathe). Long processor runs are great for standard surgical material - but absolutely overprocess small pieces of tissue, resulting in hard, dehydrated, difficult to cut little brittle fragments. The best approach that I've seen and used is to examine your "bottlenecks." The main bottlenecks are the points at which you have lots to do in front of you - with limited resource to do it (i.e. you have 150 cassettes to embed coming off of the processor at one time and ONE embedding station. That's a bottleneck.) What time do you usually start your processor? Say your processor starts at 10:00 p.m. with a standard 6 hour process run. Your pathologists or your PAs close the grossing stations at 6:00 p.m. and load the processor - and everything sits there for 4 hours (that's another bottleneck). In this scenario - you can add a second processor run at virtually any time that you are staffed that allows you to turn over the processor for the standard run by 6:00 p.m. (when the standard next load is). Since a small biopsy run usually takes about 2 hours on the Vitek: 0600 - Standard processor emptied - large surgical cases ONLY (about 2/3 of your blocks) 0600 - 1000: Embed, cut, stain and coverslip surgicals 0700 - 0800: Previous day's and early AM courier run of small biopsies grossed in 0800 - 1000: Small biopsy processor run 1000 - small biopsy processor emptied 1000 - 1230: Embed, cut, stain and coverslip biopsies. If you wanted to do another biopsy run - to allow same day turnaround time - the 2nd biopsy run could be scheduled at 10:30 each day with slides coming out around 3:30. I'm interested to hear other people's ideas. Julia >From: "Rush, Joyce" >To: >Subject: [Histonet] Long and short biopsy runs with one processor >Date: Wed, 13 Apr 2005 12:57:07 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by mc7-f23.hotmail.com >with Microsoft SMTPSVC(6.0.3790.211); Wed, 13 Apr 2005 10:58:11 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1DLm7H-0004v5-7P; Wed, 13 Apr >2005 12:57:36 -0500 >Received: from [199.165.152.166] (helo=swlx166.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1DLm6v-0004uy-Kqfor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 12:57:19 -0500 >Received: from [66.173.44.122] (helo=keys.sjmcmn.org)by swlx166.swmed.edu >with esmtp (Exim 4.44) id 1DLm6u-0004b0-5Ffor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 12:57:09 -0500 >Received: from keys.sjmcmn.org (unknown [127.0.0.1])by keys.sjmcmn.org >(Postfix) with ESMTP id 50777256FE9for >;Wed, 13 Apr 2005 12:57:07 -0500 (CDT) >Received: from mail.SJMCMN.ORG ([10.1.0.6])by keys.sjmcmn.org (PGP >Universal service);Wed, 13 Apr 2005 12:57:07 -0500 >X-Message-Info: cmh8WMAfDXqeqGy5Qdhsq/iJQn/CwXc1jm673ZQpEo0= >X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 >Content-class: urn:content-classes:message >X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: Long and short biopsy >runs with one processor >Thread-Index: AcVAUjUG93SXzh8HQoGO/FSJK5JRgA== >X-Scan-Signature: 5ce762e8aa474ea7aaa37532c083de4e >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 201832d891fa504721b2e650a5a52212 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 13 Apr 2005 17:58:11.0794 (UTC) >FILETIME=[5B956320:01C54052] > >We are a community hospital and last year processed 28,000 blocks. We are >being challenged by our Laboratory Director to run a long and short, biopsy >run daily, M-F. Currently we run everything together. We have one >processor, a VIP5. I'd love to hear of ways people have handled this. >Thanks so much! I always get such good advice from this group! > >Joyce > >Joyce A. Rush, BS, MT(ASCP) >Laboratory Manager >St Joseph's Medical Center >523 North Third Street >Brainerd, MN 56401 >Office:218-828-7500 Fax: 218-828-7510 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From slappycraw <@t> yahoo.com Wed Apr 13 16:59:36 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Long and short biopsy runs with one processor Message-ID: <20050413215936.23558.qmail@web52607.mail.yahoo.com> Look into the RTP from Sakura- it does small specimens in about an hour and you can continuously put baskets on every 15 min. Larry __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From colesy1222 <@t> yahoo.com Wed Apr 13 18:13:28 2005 From: colesy1222 <@t> yahoo.com (Nicole Puglisi) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Long and short biopsy runs with one processor In-Reply-To: <20050413215936.23558.qmail@web52607.mail.yahoo.com> Message-ID: <20050413231328.22784.qmail@web30607.mail.mud.yahoo.com> The Peloris Dual Retort Rapid Tissue Processor is also something to look into. We purchased one 2 months ago. It is half the price of the Sakura RTP. Biopsies take 1 hour, skin cases 3 hours and other routine tissues 5 hours to process. It's a great processor!! Nicole Larry Woody wrote: Look into the RTP from Sakura- it does small specimens in about an hour and you can continuously put baskets on every 15 min. Larry __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From kamalpatel9999 <@t> yahoo.com Wed Apr 13 19:39:29 2005 From: kamalpatel9999 <@t> yahoo.com (kamal patel) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Long and short biopsy runs with one processor In-Reply-To: 6667 Message-ID: <20050414003929.78288.qmail@web60612.mail.yahoo.com> Larry, I'll check it out. KP --- Larry Woody wrote: > Look into the RTP from Sakura- it does small > specimens in about an hour and you can continuously > put baskets on every 15 min. Larry > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam > protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From kamalpatel9999 <@t> yahoo.com Wed Apr 13 19:44:38 2005 From: kamalpatel9999 <@t> yahoo.com (kamal patel) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Long and short biopsy runs with one processor In-Reply-To: 6667 Message-ID: <20050414004438.34590.qmail@web60625.mail.yahoo.com> Nicole, If the Peloris is such a "great processor" whya are they being given away ? Ameripath hasn't spent a dime re Capital Equipment purchase. Do the valves still clog and need to be removed and cleaned resulting in serious down-time? Do the reagent and wax lines still "foul" ? What happens if the rotary valve breaks ? It is the ONLY valve regulating Wax and reagent movement. Is the software still problematic and prone to crashes mid-run? I think to be fair to all manufacturers, ALL the +'s and -'s for all instruments need to be taken into consideration. Speed at the cost of having a FSE sleep next to the instrument continuously is not a solution. BTW, where do you work ? Do you have a Peloris in your lab? May I contact you and ask some more questions ? KP --- Nicole Puglisi wrote: > The Peloris Dual Retort Rapid Tissue Processor is > also something to look into. We purchased one 2 > months ago. It is half the price of the Sakura RTP. > Biopsies take 1 hour, skin cases 3 hours and other > routine tissues 5 hours to process. It's a great > processor!! > > Nicole > > > Larry Woody wrote: > Look into the RTP from Sakura- it does small > specimens in about an hour and you can continuously > put baskets on every 15 min. Larry > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam > protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam > protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From kamalpatel9999 <@t> yahoo.com Wed Apr 13 19:47:42 2005 From: kamalpatel9999 <@t> yahoo.com (kamal patel) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Long and short biopsy runs with one processor In-Reply-To: <000001c54075$be561130$0ecd080a@wsahs.nsw.gov.au> Message-ID: <20050414004742.47218.qmail@web60622.mail.yahoo.com> Bill, I agree, the VIPs are good old workhorses! You may be right about the single valve, not sure what woulf happen if it dumped. You know why Vision Biosystem and Leica split ? This will explain a lot. If Peloris reliable, why can't they sell them - they're giving them away ! Not a glowing testimonial. I'll stay with the VIP. Do you know where to get one of the old TP 1050's KP --- Bill Sinai wrote: > > The Leica are lesss than two years old while the > VIPs are both early 90s > vintage but still perform well. I have looked at > the Peloris but my biggest > worry was that there is only one pump for solutions > and vacuum. So that if > either fail does that stop both chambers from being > useable? Otherwise a > very sound idea which works. I was involved in the > very early stages of the > development of this processor and they use similar > components to the Leica > TP 1050 as Vision built these for Leica until > recently so reliability should > not be a problem. > > Bill Sinai > Laboratory Manager > Tissue Pathology, ICPMR > Westmead NSW 2145 > Australia > Ph 02 9845 7774 > > > -----Original Message----- > From: kamal patel [mailto:kamalpatel9999@yahoo.com] > Sent: Thursday, 14 April 2005 8:04 AM > To: Bill Sinai > Subject: RE: [Histonet] Long and short biopsy runs > with one processor > > > Bill, > > Has your lab considered upgrading your processor(s)? > We have a VIP as well, and an autostainer but > recently > looked into the Peloris from Vision Biosystems. > Ameripath seems to have good things to say about it. > > > --- Bill Sinai wrote: > > > > > Roxanne, > > > > Most are the small GE biopsies >3mm in diamater or > > "thin" slices of any > > urgents >3mm thick. > > Solution Time > > Formalin 0min > > 70%ethanol 5min > > 95% 5min > > 100% 5min > > 100% 10min > > 100% 10min > > Xylol 5min > > Xylol 5min > > Xylol 10min > > Wax 5min > > Wax 10min > > Wax 10min > > > > Temperature ambient with Pressur/Vacuum on all > > stations. > > > > Two Leica ASP300 and two VIP 3000 > > Bill Sinai > > Laboratory Manager > > Tissue Pathology, ICPMR > > Westmead NSW 2145 > > Australia > > Ph 02 9845 7774 > > > > -----Original Message----- > > From: Roxanne Soto [mailto:godsgirlnow@MSN.COM] > > Sent: Thursday, 14 April 2005 7:37 AM > > To: Bill Sinai > > Subject: Re: [Histonet] Long and short biopsy > runs > > with one processor > > > > > > What size is the tissue on the 2 hour run? How > > long is each station? > > What kind of processor? Do you use the vacuum? > > Roxanne > > ----- Original Message ----- > > From: Bill Sinai > > To: histonet (E-mail) > > Sent: Wednesday, April 13, 2005 5:30 PM > > Subject: RE: [Histonet] Long and short biopsy > > runs with one processor > > > > > > > > Julia, > > > > I agree with your philosophy about small runs. > > We are a pathology > > department supporting a large teaching > hospital > > and an even larger area > > pathology service. However, we also do a > > considerable amount of private > > work for several endoscopy clinics in our > > locality. > > The scenario you describe is very similar to > > ours. > > We have the usual overnight run, usually > 450-600 > > blocks with the large > > material in one processor and the smaller in > > another. We receive the > > endoscopy specimens anytime from 7:30am > through > > to 3:00pm each day, with > > any > > pick ups after 6:00pm being processed the next > > morning in a short run > > 2-2.5hrs. This means we can have at least two > > runs per day of endoscopy > > specimens. The endoscopy specimens from the > > private clinics are about > > 30% > > of our work and the TAT for these specimens is > > >2 days from pick-up to > > hard > > copy result to the clinician. > > > > We also process renal biopsies several times > per > > day as well as the > > occasional urgent specimen. > > > > Bill Sinai > > Laboratory Manager > > Tissue Pathology, ICPMR > > Westmead NSW 2145 > > Australia > > Ph 02 9845 7774 > > > > > > -----Original Message----- > > From: > histonet-bounces@lists.utsouthwestern.edu > > > > > [mailto:histonet-bounces@lists.utsouthwestern.edu]On > > Behalf Of Julia > > Dahl > > Sent: Thursday, 14 April 2005 4:13 AM > > To: Joyce.Rush@sjmcmn.org; > > Histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] Long and short biopsy > > runs with one processor > > > > > > Joyce - > > > > First let me preface with my bias - I am a GI > > pathologist (read small > > biopsy > > material is what I live and breathe). > > > > Long processor runs are great for standard > > surgical material - but > > absolutely overprocess small pieces of tissue, > > resulting in hard, > > dehydrated, difficult to cut little brittle > > fragments. > > > > The best approach that I've seen and used is > to > > examine your > > "bottlenecks." > > The main bottlenecks are the points at which > you > > have lots to do in > > front of > > you - with limited resource to do it (i.e. you > > have 150 cassettes to > > embed > > coming off of the processor at one time and > ONE > > embedding station. > > That's a > > bottleneck.) > > > > What time do you usually start your processor? > > Say your processor > > starts at > > 10:00 p.m. with a standard 6 hour process run. > > Your pathologists or > > your > > PAs close the grossing stations at 6:00 p.m. > and > > load the processor - > > and > > everything sits there for 4 hours (that's > === message truncated === __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From emry <@t> u.washington.edu Thu Apr 14 07:52:01 2005 From: emry <@t> u.washington.edu (Trisha Emry) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] EDTA Message-ID: Is it necessary to use distilled H20 for EDTA or can you use tap? Is anyone using heat with EDTA? Please send details. Thank you, Trisha Emry U of WA, Seattle From kamalpatel9999 <@t> yahoo.com Thu Apr 14 03:55:50 2005 From: kamalpatel9999 <@t> yahoo.com (kamal patel) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] EDTA In-Reply-To: 6667 Message-ID: <20050414085550.61092.qmail@web60615.mail.yahoo.com> Trisha, We have successfully used Tris-EDTA (ph 9.2) as a HIER method with certain antibodies. Which in particular are you testing ? As far as the water goes, I always believe that Distilled, or even double distilled if you have it - is best for all solution preparation. Water from the faucet can introduce salts and minerals that may affect your solution's abilities. KP --- Trisha Emry wrote: > Is it necessary to use distilled H20 for EDTA or can > you use tap? > > Is anyone using heat with EDTA? Please send > details. > > Thank you, > > Trisha Emry > U of WA, Seattle > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From sayanarra <@t> yahoo.com Thu Apr 14 04:12:19 2005 From: sayanarra <@t> yahoo.com (saya narra) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] EDTA Message-ID: <20050414091219.64929.qmail@web60615.mail.yahoo.com> Kamal, We have used tap water for our solution prep with no ill effects. Purifyers and bottled from store are expensive. Saya Narra UofT __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From sayanarra <@t> yahoo.com Thu Apr 14 04:42:40 2005 From: sayanarra <@t> yahoo.com (saya narra) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Processors Message-ID: <20050414094240.86704.qmail@web60616.mail.yahoo.com> Hello Bill, You said your VIP was reliable . I agree, we have one too and it is a good instrument. It seems no one may get a chance to get a Peloris as the competition says Vision Biosystems is FOR SALE or looking for a distributor in the USA. You are in Australia and the Histology field. Thie website says they are in Mt.Waverly, VIC, Australia. Is that near you? Have you heard any word on their Boss going door to door looking for a buyer in the USA ? Saya Narra UofT __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From GDawson <@t> dynacaremilwaukee.com Thu Apr 14 07:37:12 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Re: Cost of automated immunos? Message-ID: In terms of cost, DAKO wins hands down. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: rgrow@bmnet.com [mailto:rgrow@bmnet.com] Sent: Wednesday, April 13, 2005 10:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Cost of automated immunos? I vote for Dako, too Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ------------------------------ Message: 23 Date: Wed, 13 Apr 2005 10:13:12 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Cost of automated immunos? To: "Sebree Linda A." , , Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA45947@sjhaexc02.sjha.org> Content-Type: text/plain; charset="iso-8859-1" Also, DAKO. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sebree Linda A. Sent: Wednesday, April 13, 2005 8:49 AM To: Krat18@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cost of automated immunos? Chris, I suggest contacting Ventana, they can give you a pretty accurate cost estimate per slide. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Krat18@aol.com Sent: Wednesday, April 13, 2005 3:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost of automated immunos? Our lab is starting to think about doing automated immunos. Can anyone out there is Histoland give me an idea of the COST of doing an automated immuno vs. a manual one? I know there are many variables, but we're looking at the Benchmark, so any idea of the cost per test on that machine? We would appreciate any input. Thanks in advance! Chris Davis _chrisdavis@hotmail.com_ (mailto:chrisdavis@hotmail.com) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. ------------------------------ Message: 24 Date: Wed, 13 Apr 2005 07:17:47 -0700 (PDT) From: Histology SLU Subject: RE: [Histonet] Cost of automated immunos? To: "Weems, Joyce" , "Sebree Linda A." , Krat18@aol.com, histonet@lists.utsouthwestern.edu Message-ID: <20050413141747.63770.qmail@web51006.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii I second DAKO. "Weems, Joyce" wrote:Also, DAKO. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sebree Linda A. Sent: Wednesday, April 13, 2005 8:49 AM To: Krat18@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cost of automated immunos? Chris, I suggest contacting Ventana, they can give you a pretty accurate cost estimate per slide. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Krat18@aol.com Sent: Wednesday, April 13, 2005 3:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost of automated immunos? Our lab is starting to think about doing automated immunos. Can anyone out there is Histoland give me an idea of the COST of doing an automated immuno vs. a manual one? I know there are many variables, but we're looking at the Benchmark, so any idea of the cost per test on that machine? We would appreciate any input. Thanks in advance! Chris Davis _chrisdavis@hotmail.com_ (mailto:chrisdavis@hotmail.com) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Thu Apr 14 08:16:15 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Long and short biopsy runs with one processor Message-ID: <20050414131615.55212.qmail@web52602.mail.yahoo.com> The Sakura RTP also does not use formalin or xylene and the longest run is only 2 hours. It comes with a grossing unit that grosses the tissue to the correct size and thickness for the processor. Loading baskets on every 15 min. means you have tissue continuously coming off from the time surgery starts in the AM and most of the specimens can be read out the same day. I have used this processor and it is really a huge advancement in tissue processing. A doctor in Miami developed it-Dr. Morales. Larry --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From Rick.Couture <@t> vision-bio.com Thu Apr 14 08:20:39 2005 From: Rick.Couture <@t> vision-bio.com (Rick Couture) Date: Fri Sep 16 15:24:57 2005 Subject: [Histonet] Cost of automated immunos Message-ID: <000d01c540f4$c0a083b0$100318ac@visionusa.com.visionusa.com> You can contact Vision Biosystems also to get pricing on our new Bond-Max immunostainer. Rick Couture Technical Service Manager Vision Biosystems 700 Longwater Drive Norwell Ma. 02061 781-616-1135 Rick.Couture@vision-bio.com From Rick.Couture <@t> vision-bio.com Thu Apr 14 08:31:53 2005 From: Rick.Couture <@t> vision-bio.com (Rick Couture) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Vision Biosystems alive and well Message-ID: <001201c540f6$520c7dd0$100318ac@visionusa.com.visionusa.com> I wish to respond to a message from saya nara. Vision Biosystems is now being distributed in the U.S. They are not for sale and you can get a Peloris if you wish. We also have a new IHC autostainer , called the Bond-Max. Whoever the "competition" is that is telling you this in error. Rick Couture Technical Service Manager Vision Biosystems 700 Longwater Drive Norwell Ma. 02061 781-616-1135 Rick.Couture@vision-bio.com From Rcartun <@t> harthosp.org Thu Apr 14 08:51:57 2005 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Isopropyl (again) Message-ID: We do not use the HercepTest kit for HER-2 IHC detection and I am not aware of any problems regarding patient treatment or pathologist/oncologist/patient reimbursement. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Katri Tuomala" 04/13/05 11:37AM >>> Also, remember, if Her2 is requested later on, Dako's Her2 Kit recommends minimum 24 hour fixation in NBF for results to be valid and reliable. I understand in US, in order for the patient to be treated and reembursed by insurance companies, Dako Kit must be used. Patient comes first, not the TAT. My two cents worth... Katri Katri Tuomala Hamilton, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------- Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From JWEEMS <@t> sjha.org Thu Apr 14 09:11:22 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Isopropyl (again) Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA4598C@sjhaexc02.sjha.org> Some clincial trials will not allow patients unless their testing has been done with FDA approved method. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Thursday, April 14, 2005 9:52 AM To: katri@cogeco.ca; Histonet@lists.utsouthwestern.edu Subject: Re: re:[Histonet] Isopropyl (again) We do not use the HercepTest kit for HER-2 IHC detection and I am not aware of any problems regarding patient treatment or pathologist/oncologist/patient reimbursement. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Katri Tuomala" 04/13/05 11:37AM >>> Also, remember, if Her2 is requested later on, Dako's Her2 Kit recommends minimum 24 hour fixation in NBF for results to be valid and reliable. I understand in US, in order for the patient to be treated and reembursed by insurance companies, Dako Kit must be used. Patient comes first, not the TAT. My two cents worth... Katri Katri Tuomala Hamilton, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------- Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From MSonkin <@t> ameripath.com Tue Apr 12 12:55:42 2005 From: MSonkin <@t> ameripath.com (Sonkin, Mikhanie) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] nucleus details Message-ID: The last 3 months we have problem with nucleus staining for gastro-intestinal bx, and liver bx. The nuclei looks "milky", without any details. It does not happened with all tissue: 2-3 blocks from 50-100. And it is not every day. And it is happened with tissue into screen cassettes only. So we have suggetion that it may air incide the cassettes that prevent tissue from full processing. Did somebody have the same problem? I will be glad to hear any suggetions. Sincerely, Misha Sonkin P.S. Sorry for my English From Margaret.Perry <@t> sdstate.edu Tue Apr 12 17:04:02 2005 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Mallory PTAH and Zenkers Message-ID: Hello, I have a question about the Mallory's PTAH. If I use the zenkers without mercuric chloride do I still need to use Gram's iodine and sodium thiosulfate? I assume not but need to be sure. Thanks for the help. Margaret Perry HT South Dakota University Veterinary Science Histopathology Brookings SD From cmilne <@t> asterand.com Wed Apr 13 14:14:27 2005 From: cmilne <@t> asterand.com (Christine Milne) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Asterand Histology Technician Position Message-ID: <56E63440ABF1FC4897947D5BA4CFA44F04CD15D0@ATL1VEXC005.usdom004.tco.tc> Nancy Lemke, B.A., HT, HTL(ASCP), is now an employee of Asterand. She suggested I should post this message for an individual who might be looking for a Histology Technician position that we have open at Asterand. This position is for a full-time Histology Technician that we need immediately! Asterand (www.asterand.com ) is a privately held company founded in early 2000 to address the urgent need of genomics and proteomics researchers for high quality biological information and materials. Working to the highest ethical and quality standards, we provide the information and materials that are needed to conduct research on common disease targets, such as cancer, heart disease and brain disorders. Our customers include researchers working at the world's foremost and oldest pharmaceutical, biotech, genomic and proteonic companies. We have partnerships with hospitals and medical institutions around the world. Our headquarters are located in the TechOne Building at Wayne State University in Detroit, MI. Asterand was voted the #4 "Top 10 Small Companies, Best Places to Work in the Biotechnolgy Industry", globally, by The Scientist magazine for 2004. We have also heard we are again in the Top Ten. We have a full range of benefits including choice of health coverages, prescription drug plan, vision care, dental, life insurance, AD&D, long-term disability, 401K plan, a stock option plan and a generous paid time off plan. POSITION SUMMARY: Prepares tissue specimens for routine and special procedures to confirm tissue diagnosis. Performs complex histological procedures, records and analyzes data, maintains and repairs instruments. Relies on experience and judgment to pan and accomplish goals. Works under general supervision. POSITION DUTIES AND RESPONSIBILTIES: * Slide preparation for Pathology review * Section slides from both paraffin embedded and OCT embedded tissues * Cryosectioning, Microtome sectioning and tissue staining and cover slipping * Data entry and tracking of all products derived from sectioning * General laboratory and equipment maintenance * Potential TMA construction * Custom sectioning for sales orders * Other duties as assigned. MINIMUM EXPERIENCE AND/OR EDUCATION REQUIRED: * Certification for Histotechnician HT * Requires Associate's degree or its equivalent * 2 to 4 years of experience in the field or related area PERSONAL ATTRIBUTES: * Excellent written and verbal communication skills * Interpersonal skills * Organizational skills and attention to detail * Problem solving skills * Passion for excellence PHYSCIAL DEMANDS: Sitting or standing for long periods of time If you know of someone looking to join a wonderful company, please have them send their resume to me, cmilne@asterand.com . I appreciate any help! Thank you, Christine Milne Asterand, Inc. H.R. Manager TechOne Suite 501 440 Burroughs Street Detroit, MI 48202 Direct Dial: 313-263-0951 Fax: 313-263-0961 Main Number: 313-263-0960 cmilne@asterand.com This e-mail message, together with any attachments, contains information belonging to Asterand, Inc. that may be confidential, proprietary, copyrighted and/or legally protected or privileged, and is intended for the sole use of the individual or entity named on this message. If you are not the intended recipient, and have received this e-mail message in error, please immediately return this message to its original sender and permanently delete it from your electronic and paper records. Thank You. From Dorothy.L.Webb <@t> HealthPartners.Com Thu Apr 14 09:30:46 2005 From: Dorothy.L.Webb <@t> HealthPartners.Com (Dorothy.L.Webb@HealthPartners.Com) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Tween charge Message-ID: Does anyone know of the "charge" that should be used for softening toe or fingernails to prepare for processing? Could a decal charge be safely used? It seems to me that with taking the time and the solutions involved, there should be some sort of charge incurred. Any thoughts and thanks much!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From TMcNemar <@t> lmhealth.org Thu Apr 14 09:32:43 2005 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Histology position Message-ID: <6CD94D97ED7D924BA5C2B588FA9528213967AC@nt_exchange> We have an opening for a Histology Tech, full time days, no weekends or holidays. Registered or registry eligible under the Jan 2005 guidelines. We are a small but progressive lab with 3 pathologists. Located about 30 east of Columbus, Ohio. If interested, please respond by email to: TMCNEMAR@LMHEALTH.ORG Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio 43055 From rocan <@t> mac.com Thu Apr 14 09:42:38 2005 From: rocan <@t> mac.com (Rocan) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Universal mouse marker Message-ID: Hello everyone, I am doing some studies using chimeras One of the species I am using is mouse. I am wondering if there is a monoclonal (rat or hamster) that would recognize every cell of murine origin. In other words a Pan-murine antibody. Perhaps anti-histocompatibility ? Maybe is quite obvious but I can not think of anything. Your suggestions and your help are welcomed. Thank you in advance for your help. Rocio ----- Dr.Rocio Sierra-Honigmann Engineered Wound Repair Laboratory Cedars Sinai Research Institute 310-423-1882 Honigmannr@cshs.org From JWEEMS <@t> sjha.org Thu Apr 14 09:57:48 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Gary Gill Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA4598E@sjhaexc02.sjha.org> Excuse me everyone - I need to reach Gary. Are you there or does someone have a current email address? Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. -------------- next part -------------- Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From RCazares <@t> schosp.org Thu Apr 14 10:08:14 2005 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Nuclear bubbling in rapid processed biopsies Message-ID: <913FAC2B773C19488E26AE6572180FA501A96C6D@exch01.schosp.org> Hi all, I have a question for those using rapid biopsy programs. How long does the biopsy fix in formalin before starting the program? ( I'm interested in the ones that come in during the work day and obviously not the one that have sat in formalin overnight). Is there a minimum time for good results? Does anyone see bubbling of the nucleus in these rapid processed samples? I too am trying to modify the way we do things in our lab to accommodate the workload increase, but occasionally I see nuclear bubbling. Any thoughts, ideas or suggestions? Ruth Cazares *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From JWEEMS <@t> sjha.org Thu Apr 14 10:11:37 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Tween charge Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA45990@sjhaexc02.sjha.org> To my knowledge there is no way to charge for anything other than decal. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dorothy.L.Webb@HealthPartners.Com Sent: Thursday, April 14, 2005 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tween charge Does anyone know of the "charge" that should be used for softening toe or fingernails to prepare for processing? Could a decal charge be safely used? It seems to me that with taking the time and the solutions involved, there should be some sort of charge incurred. Any thoughts and thanks much!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From Janet.Bonner <@t> FLHOSP.ORG Thu Apr 14 10:37:14 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Vision Biosystems alive and well Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4237@fh2k093.fhmis.net> "not for sale"? Why worry about the competition? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu To: Histonet Sent: 4/14/2005 9:31 AM Subject: [Histonet] Vision Biosystems alive and well I wish to respond to a message from saya nara. Vision Biosystems is now being distributed in the U.S. They are not for sale and you can get a Peloris if you wish. We also have a new IHC autostainer , called the Bond-Max. Whoever the "competition" is that is telling you this in error. Rick Couture Technical Service Manager Vision Biosystems 700 Longwater Drive Norwell Ma. 02061 781-616-1135 Rick.Couture@vision-bio.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Apr 14 10:44:48 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:58 2005 Subject: For what purpose? Re: [Histonet] EDTA In-Reply-To: References: Message-ID: <6.0.0.22.1.20050414094247.01b556a8@gemini.msu.montana.edu> For what purpose, antigen retrieval OR decalcification of bone? Personally, any solutions made up in our go into distilled or equivalent water, tap water is reserved for rinsing sections or NBF fixed bone samples. At 06:52 AM 4/14/2005, you wrote: >Is it necessary to use distilled H20 for EDTA or can you use tap? > >Is anyone using heat with EDTA? Please send details. > >Thank you, > >Trisha Emry >U of WA, Seattle > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From dpahisto <@t> yahoo.com Thu Apr 14 11:19:51 2005 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Her2 testing Message-ID: <20050414161951.64144.qmail@web32107.mail.mud.yahoo.com> Is it still true that only the Dako Her2 is FDA approved? If not, what everyone's preference for this antibdody? Cindy DuBois __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From m.wei <@t> biogenex.com Thu Apr 14 11:34:17 2005 From: m.wei <@t> biogenex.com (May Wei) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Her2 testing Message-ID: <37DC9F93CF7F864182D0463EF93D571B0431EE@ISLETON2.california.biogenex.com> Dear Cindy, BioGenex's InSite Her-2/neu Kit is FDA approved. It is CB11 clone kit. May Wei, M. Med., MBA Sales Manager, International BioGenex Laboratories, Inc. 4600 Norris Canyon Road San Ramon, CA 94583 Tel: 1 800 421 4149 Fax: 1 925 866 2525 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Thursday, April 14, 2005 9:20 AM To: Histonet Subject: [Histonet] Her2 testing Is it still true that only the Dako Her2 is FDA approved? If not, what everyone's preference for this antibdody? Cindy DuBois __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Thu Apr 14 11:55:32 2005 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Ulex Europeaus for Ventana instruments Message-ID: I'm looking for a Ulex Europeaus antibody that will work on Ventana automated instruments. Any suggestions are appreciated. Thank you, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From jkiernan <@t> uwo.ca Thu Apr 14 11:58:24 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] EDTA --> pure water References: Message-ID: <425EA130.CBBBA9BC@uwo.ca> When instructions specify "water," without qualification, it is usual to use purified water. If tap water (whatever might be dissolved in it) is OK, the instructions will usually say "tap water" explicitly. EDTA has various uses; in histological work they mostly involve removal of calcium ions. If your tap water is hard (= contains calcium ions), it will use up some of the EDTA in the solution. If you know the Ca content of the tap water you can calculate the equivalent amount of wasted EDTA. One mole of calcium ions (40 grams) combines with one mole of disodium EDTA dihydrate (372 grams). For example, if a litre of tap water contains 100mg of calcium, that will consume 100X372/40 =930mg of EDTA. This would reduce the available EDTA in a 1% solution to about 0.9%. I do not know if my guess of 100ppm Ca is anywhere near reasonable for hard water, or if a certificate of tap water analysis reports the calcium content in this way. If my guesstimate is too low, hard water may eat up more than 10% of the EDTA in a 1% solution. A simple test for minimally "clean" lab water is to mix it with an equal volume of 1% aqueous silver nitrate. Any precipitate or opalescence indicates that the water contains anions with insoluble Ag salts or organic compounds that reduce Ag+. John Kiernan Anatomy, UWO, London, Canada. _______________________________________ Trisha Emry wrote: > > Is it necessary to use distilled H20 for EDTA or can you use tap? > > Is anyone using heat with EDTA? Please send details. > > Thank you, > > Trisha Emry > U of WA, Seattle ________________________________ From gcallis <@t> montana.edu Thu Apr 14 12:49:30 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Incredible book on mouse anatomy Message-ID: <6.0.0.22.1.20050414114731.01b06fd0@gemini.msu.montana.edu> A Color Atlas of Sectional Anatomy of the Mouse by Takamasa Iwaki, Hiroshi Yamashita, Toshiyuki Hayakawa Published in Japan in 15th September 2001 185 Pages Type: hardcover It costs $175.00, but is filled with spectacular color photos, text is in English and Japanese, Go to Braintree Scientific Website Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From p.rastogi <@t> biogenex.com Thu Apr 14 13:08:30 2005 From: p.rastogi <@t> biogenex.com (Promila Rastogi) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Her2 testing Message-ID: <37DC9F93CF7F864182D0463EF93D571B034B40@ISLETON2.california.biogenex.com> FYI, BioGenex recently received FDA approval for our InSite HER2/neu kit. Promila Rastogi Reagents Product Manager >Message: 17 >Date: Thu, 14 Apr 2005 09:19:51 -0700 (PDT) >From: Cindy DuBois >Subject: [Histonet] Her2 testing >To: Histonet >Message-ID: <20050414161951.64144.qmail@web32107.mail.mud.yahoo.com> >Content-Type: text/plain; charset=us-ascii >Is it still true that only the Dako Her2 is FDA approved? >If not, what everyone's preference for this antibdody? >Cindy DuBois From twebster <@t> nmcinc.org Thu Apr 14 13:17:22 2005 From: twebster <@t> nmcinc.org (Tim Webster) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Source for adjustable workstations? Message-ID: Hi All, A recent ergonomic assessment of our Histo Lab produced a seven page litany of ergonomic ills (Yay!) As we are about to renovate in stages, I was looking for a source of adjustable tables/benches/stations for embedding and microtomy. Staff height varies from 5'2" to 6'1+3/4. Adjusting the height of the chair is inadequate for the height of the benches and equipment. I have one supplier of adjustable tables (Custom-products.com) I would love someone to offer another name. (I struggle finding stuff on the web sometimes . . .) Also, how do you folks handle ergonomics for routine histo? Have a great day Tim Tim Webster Histology Specialist Northwestern Medical Center 133 Fairfield Street St Albans, VT 05478 (802) 524-1070 twebster@nmcinc.org From kbroomal <@t> NEMOURS.ORG Thu Apr 14 13:29:44 2005 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Source for adjustable workstations? Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A79B5@wlmmsx01.nemours.org> "Also, how do you folks handle ergonomics for routine histo?" Ibuprofen and muscle relaxers. Sorry, I couldn't resist. (I'm not making it up though!) But seriously, I'm 5'2" & get horrible muscle spasms in my shoulder from sitting at the microtome for long periods at a time, no matter how high the chair is positioned. I must stretch my body in some funky direction to reach the wheel. I've gotten used to using the automated part of the microtome with the foot pedal. I'd love to hear what you come up with! Kristen Broomall -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tim Webster Sent: Thursday, April 14, 2005 2:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Source for adjustable workstations? Hi All, A recent ergonomic assessment of our Histo Lab produced a seven page litany of ergonomic ills (Yay!) As we are about to renovate in stages, I was looking for a source of adjustable tables/benches/stations for embedding and microtomy. Staff height varies from 5'2" to 6'1+3/4. Adjusting the height of the chair is inadequate for the height of the benches and equipment. I have one supplier of adjustable tables (Custom-products.com) I would love someone to offer another name. (I struggle finding stuff on the web sometimes . . .) Also, how do you folks handle ergonomics for routine histo? Have a great day Tim Tim Webster Histology Specialist Northwestern Medical Center 133 Fairfield Street St Albans, VT 05478 (802) 524-1070 twebster@nmcinc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Apr 14 13:34:28 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Tween charge In-Reply-To: Message-ID: I think this would fall under the category of soaking, like overdehydrated blocks. There is no charge for this. Dorothy.L.Webb@HealthPartners.Com Sent by: histonet-bounces@lists.utsouthwestern.edu 04/14/2005 07:30 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Tween charge Does anyone know of the "charge" that should be used for softening toe or fingernails to prepare for processing? Could a decal charge be safely used? It seems to me that with taking the time and the solutions involved, there should be some sort of charge incurred. Any thoughts and thanks much!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DavidH <@t> marketlabinc.com Thu Apr 14 13:43:23 2005 From: DavidH <@t> marketlabinc.com (David Haagsma) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Source for adjustable workstations? Message-ID: <19E3602A16438E48B51A4250CA04B5F60F98CC@exchange.marketlab.com> Tim, MarketLab Inc. has adjustable microtome stations, http://www.marketlabinc.com/products/product.cfm/ML3158 And adjustable height grossing stations http://www.marketlabinc.com/products/product.cfm/ML3217 http://www.marketlabinc.com/products/categorydetail.cfm/722 And Microtome Arm Rests http://www.marketlabinc.com/products/product.cfm/ML1038L Thank you, Dave Haagsma www.marketlabinc.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Webster Sent: Thursday, April 14, 2005 2:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Source for adjustable workstations? Hi All, A recent ergonomic assessment of our Histo Lab produced a seven page litany of ergonomic ills (Yay!) As we are about to renovate in stages, I was looking for a source of adjustable tables/benches/stations for embedding and microtomy. Staff height varies from 5'2" to 6'1+3/4. Adjusting the height of the chair is inadequate for the height of the benches and equipment. I have one supplier of adjustable tables (Custom-products.com) I would love someone to offer another name. (I struggle finding stuff on the web sometimes . . .) Also, how do you folks handle ergonomics for routine histo? Have a great day Tim Tim Webster Histology Specialist Northwestern Medical Center 133 Fairfield Street St Albans, VT 05478 (802) 524-1070 twebster@nmcinc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sayanarra <@t> yahoo.com Thu Apr 14 13:46:26 2005 From: sayanarra <@t> yahoo.com (saya narra) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] "Vision Biosystems is now being distributed in the US" Message-ID: <20050414184626.52381.qmail@web60620.mail.yahoo.com> Hi Rick ! It appears your automated immunos are overpriced compared to Dako. As a general rule, it's not good to advertise what isn't competitive in a given market. Business 101. Also, you said " Vision Biosystems is now being distributed in the U.S. ". Who is the distributor ? Can you get me the Company info so I may contact them re pricing, service etc. I wasn't aware that Vision Biosystems had found a "distributor". What is the website of the Distributor ? Is it Leica ? Saya Narra UofT __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From slappycraw <@t> yahoo.com Thu Apr 14 13:49:58 2005 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Source for adjustable workstations? Message-ID: <20050414184959.12724.qmail@web52606.mail.yahoo.com> I have degenerative disc disease in my neck from 25 yrs of cutting but what may surprise you is that standing up to cut bothers me a whole lot less than sitting down, and it is less pressure on the back to stand up and I use a lot less ibuprofen. Larry --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! From ja.mitchell <@t> hosp.wisc.edu Thu Apr 14 14:29:14 2005 From: ja.mitchell <@t> hosp.wisc.edu (Mitchell Jean A.) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Timer Search Message-ID: <583D3E9A1E843445BD54E461D1A2F6F30F83F596@uwhis-xchng2.hosp.wisc.edu> I am on a search for the large (approx 3 1/2 in square) grey winding (120 minute) timers that were ever so popular in the lab once upon a time. Anyone have a clue if they are still available anywhere? I've been searching for awhile & can't come up with a source. Thanks much, Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Department of Neurology, Neuromuscular Laboratory Madison, WI From carolb <@t> mail.phys.mcw.edu Thu Apr 14 15:30:39 2005 From: carolb <@t> mail.phys.mcw.edu (Carol Bobrowitz) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Microm IHC stainer Message-ID: <59DF6640CDC0D7119DF000D0B761393C1A965A@thor.phys.mcw.edu> I'm curious. Any Histology techs out there that have demo'd the Microm IHC stainer? What are your comments? If Richard Allan was purchased by Fisher, who does the repairs and is there a yearly service contract? Vendors, Please do not send me any information. I would like comments from users not a sales pitch. Thanks, Carol Ann Bobrowitz Histology Laboratory Department of Physiology - Room 541 Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, Wisconsin 53226 414-456-8179 FAX 414-456-6546 From bills <@t> icpmr.wsahs.nsw.gov.au Thu Apr 14 16:24:48 2005 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] RE: Processors In-Reply-To: <20050414094240.86704.qmail@wsahs.nsw.gov.au> Message-ID: <001401c54138$632f85c0$0ecd080a@wsahs.nsw.gov.au> I believe they are looking for a distributor in the USA. I have heard no rumour about the company being for sale. Histology equipment is but a small part of their whole business. Mt Waverley is in Victoria (the smallest state in Australia) I am in the outer suburbs of Sydney in NSW. The distance between is 1000klm (700miles) Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: saya narra [mailto:sayanarra@yahoo.com] Sent: Thursday, 14 April 2005 7:43 PM To: histonet@lists.utsouthwestern.edu Cc: bills@icpmr.wsahs.nsw.gov.au Subject: Processors Hello Bill, You said your VIP was reliable . I agree, we have one too and it is a good instrument. It seems no one may get a chance to get a Peloris as the competition says Vision Biosystems is FOR SALE or looking for a distributor in the USA. You are in Australia and the Histology field. Thie website says they are in Mt.Waverly, VIC, Australia. Is that near you? Have you heard any word on their Boss going door to door looking for a buyer in the USA ? Saya Narra UofT __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From bills <@t> icpmr.wsahs.nsw.gov.au Thu Apr 14 16:35:31 2005 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Long and short biopsy runs with one processor In-Reply-To: Message-ID: <001501c54139$e27d9730$0ecd080a@wsahs.nsw.gov.au> We occasionally run cell blocks but most times they are put through the routine overnight processing. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: dahmed@mdanderson.org [mailto:dahmed@mdanderson.org] Sent: Thursday, 14 April 2005 11:13 PM To: Bill Sinai Subject: RE: [Histonet] Long and short biopsy runs with one processor What about cell blocks? Do you feel that your currenct processing schedule for biopsies would be appropriate for cytology cell blocks? Our current bx/cell block protocol is 20 minutes in all stations including formalin for a total run time of 6 hours? Can I trim this back a little? David S. Ahmed Chief Histology Technician Department of Pathology M.D. Anderson Cancer Center "Bill Sinai" To: Sent by: "'Roxanne Soto'" , "histonet (E-mail)" histonet-bounces@lists.utsouth western.edu cc: 04/13/2005 04:55 PM Subject: RE: [Histonet] Long and short biopsy runs with one processor Roxanne, Most are the small GE biopsies >3mm in diamater or "thin" slices of any urgents >3mm thick. Solution Time Formalin 0min 70%ethanol 5min 95% 5min 100% 5min 100% 10min 100% 10min Xylol 5min Xylol 5min Xylol 10min Wax 5min Wax 10min Wax 10min Temperature ambient with Pressur/Vacuum on all stations. Two Leica ASP300 and two VIP 3000 Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: Roxanne Soto [mailto:godsgirlnow@MSN.COM] Sent: Thursday, 14 April 2005 7:37 AM To: Bill Sinai Subject: Re: [Histonet] Long and short biopsy runs with one processor What size is the tissue on the 2 hour run? How long is each station? What kind of processor? Do you use the vacuum? Roxanne ----- Original Message ----- From: Bill Sinai To: histonet (E-mail) Sent: Wednesday, April 13, 2005 5:30 PM Subject: RE: [Histonet] Long and short biopsy runs with one processor Julia, I agree with your philosophy about small runs. We are a pathology department supporting a large teaching hospital and an even larger area pathology service. However, we also do a considerable amount of private work for several endoscopy clinics in our locality. The scenario you describe is very similar to ours. We have the usual overnight run, usually 450-600 blocks with the large material in one processor and the smaller in another. We receive the endoscopy specimens anytime from 7:30am through to 3:00pm each day, with any pick ups after 6:00pm being processed the next morning in a short run 2-2.5hrs. This means we can have at least two runs per day of endoscopy specimens. The endoscopy specimens from the private clinics are about 30% of our work and the TAT for these specimens is >2 days from pick-up to hard copy result to the clinician. We also process renal biopsies several times per day as well as the occasional urgent specimen. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Julia Dahl Sent: Thursday, 14 April 2005 4:13 AM To: Joyce.Rush@sjmcmn.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Long and short biopsy runs with one processor Joyce - First let me preface with my bias - I am a GI pathologist (read small biopsy material is what I live and breathe). Long processor runs are great for standard surgical material - but absolutely overprocess small pieces of tissue, resulting in hard, dehydrated, difficult to cut little brittle fragments. The best approach that I've seen and used is to examine your "bottlenecks." The main bottlenecks are the points at which you have lots to do in front of you - with limited resource to do it (i.e. you have 150 cassettes to embed coming off of the processor at one time and ONE embedding station. That's a bottleneck.) What time do you usually start your processor? Say your processor starts at 10:00 p.m. with a standard 6 hour process run. Your pathologists or your PAs close the grossing stations at 6:00 p.m. and load the processor - and everything sits there for 4 hours (that's another bottleneck). In this scenario - you can add a second processor run at virtually any time that you are staffed that allows you to turn over the processor for the standard run by 6:00 p.m. (when the standard next load is). Since a small biopsy run usually takes about 2 hours on the Vitek: 0600 - Standard processor emptied - large surgical cases ONLY (about 2/3 of your blocks) 0600 - 1000: Embed, cut, stain and coverslip surgicals 0700 - 0800: Previous day's and early AM courier run of small biopsies grossed in 0800 - 1000: Small biopsy processor run 1000 - small biopsy processor emptied 1000 - 1230: Embed, cut, stain and coverslip biopsies. If you wanted to do another biopsy run - to allow same day turnaround time - the 2nd biopsy run could be scheduled at 10:30 each day with slides coming out around 3:30. I'm interested to hear other people's ideas. Julia >From: "Rush, Joyce" >To: >Subject: [Histonet] Long and short biopsy runs with one processor >Date: Wed, 13 Apr 2005 12:57:07 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by mc7-f23.hotmail.com >with Microsoft SMTPSVC(6.0.3790.211); Wed, 13 Apr 2005 10:58:11 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1DLm7H-0004v5-7P; Wed, 13 Apr >2005 12:57:36 -0500 >Received: from [199.165.152.166] (helo=swlx166.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1DLm6v-0004uy-Kqfor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 12:57:19 -0500 >Received: from [66.173.44.122] (helo=keys.sjmcmn.org)by swlx166.swmed.edu >with esmtp (Exim 4.44) id 1DLm6u-0004b0-5Ffor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 12:57:09 -0500 >Received: from keys.sjmcmn.org (unknown [127.0.0.1])by keys.sjmcmn.org >(Postfix) with ESMTP id 50777256FE9for >;Wed, 13 Apr 2005 12:57:07 -0500 (CDT) >Received: from mail.SJMCMN.ORG ([10.1.0.6])by keys.sjmcmn.org (PGP >Universal service);Wed, 13 Apr 2005 12:57:07 -0500 >X-Message-Info: cmh8WMAfDXqeqGy5Qdhsq/iJQn/CwXc1jm673ZQpEo0= >X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 >Content-class: urn:content-classes:message >X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: Long and short biopsy >runs with one processor >Thread-Index: AcVAUjUG93SXzh8HQoGO/FSJK5JRgA== >X-Scan-Signature: 5ce762e8aa474ea7aaa37532c083de4e >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >,< mailto:histone t-request@lists.utsouthwestern.edu?subject=subscribe> >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 201832d891fa504721b2e650a5a52212 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 13 Apr 2005 17:58:11.0794 (UTC) >FILETIME=[5B956320:01C54052] > >We are a community hospital and last year processed 28,000 blocks. We are >being challenged by our Laboratory Director to run a long and short, biopsy >run daily, M-F. Currently we run everything together. We have one >processor, a VIP5. I'd love to hear of ways people have handled this. >Thanks so much! I always get such good advice from this group! > >Joyce > >Joyce A. Rush, BS, MT(ASCP) >Laboratory Manager >St Joseph's Medical Center >523 North Third Street >Brainerd, MN 56401 >Office:218-828-7500 Fax: 218-828-7510 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From sayanarra <@t> yahoo.com Thu Apr 14 20:20:11 2005 From: sayanarra <@t> yahoo.com (saya narra) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Distributor Message-ID: <20050415012011.27372.qmail@web60617.mail.yahoo.com> Rick Couture, Thank you for the information. I will speak with our Lab Manager and have her contact the Distributor of the Vision Biosystem Instruments directly, should we decide on the need to purchase. Who was the Distributor you spoke of again ? saya narra UofT __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ From JMyers1 <@t> aol.com Thu Apr 14 23:48:07 2005 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Re: Histonet Posting - Tampa Area Message-ID: <1ab.36836df6.2f90a187@aol.com> Carol: I am quite familiar with this institution; I worked as the lab manager in another hospital that's just a few miles away, and I've known several of the techs who have worked here. Please feel free to call/write me... Joe Myers, M.S., CT(ASCP) Clearwater, FL 727-504-0250 ******************************** Message: 8 Date: Wed, 13 Apr 2005 14:09:01 -0500 From: cjohnston@mdanderson.org Subject: [Histonet] Tampa Area To: histonet@pathology.swmed.edu I am looking for anyone familiar with Sun Coast Hospital in the Tampa area. If you could email me privately, I would appreciate it . Carol M. Johnston HT(ASCP) Division of Surgery M.D. Anderson Cancer Center 1515 Holcombe Blvd. Unit 017 Houston, Texas 77030 713-745-4625 fax: 713-745-2548 From schmitzn <@t> uni-muenster.de Fri Apr 15 02:54:26 2005 From: schmitzn <@t> uni-muenster.de (Schmitz, Nicole) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] RNA in situ hybridization-staining problems Message-ID: <320709412D172C48A73DA1D2D0BE20820184AFD1@UKMSRV032.ukmuenster.de> In our lab we perform RNA in situ hybridizations on paraffin sections. We use the Roche DIG Labelling Kit and the Nucleic Detection Kit (AP-conjugated antibody and NBT/BCIP staining). We tried to optimize the protocol but there are still some problems occuring. After getting rid of the background staining, which took quite a while we now have the problem that after the stopping of the final staining reaction the sections go on turning darker and darker over the next few days. Even the sense probe turns dark and if there is a high mRNA expression the sections sometimes turn so dark that you can see nothing anymore except the dark colour. Has anyone encountered that kind of problem before and has any suggestions? The problem is that we have to store the slides so just taking pictures right after staining is not the best solution.. And I have another question. While trying to solve the problem I found out that some people use an Poly d(T) oligonucleotide to measure RNA degradation in the tissue. Does anyone know more about that e.g. where to order and how to use? Would I have to use another protocol for that than my normal in situ protocol? Thank you for your help Sincerely, Nicole Schmitz From emerald_lake77 <@t> yahoo.com Fri Apr 15 05:42:50 2005 From: emerald_lake77 <@t> yahoo.com (- -) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] RNA in situ hybridization-staining problems In-Reply-To: <320709412D172C48A73DA1D2D0BE20820184AFD1@UKMSRV032.ukmuenster.de> Message-ID: <20050415104250.78402.qmail@web42302.mail.yahoo.com> Nicole, I encountered the same problem and switched to the HRP-conjugated anti-DIG antibody also by Roche. I used a TSA amplification system (Biogenex - mainly used for their machines, but I was able to use it by hand also) and finally, detected with DAB. All my samples come out great! There was need for some adjustment as I was getting background initially. But I got rid of that with time adjustment and blocking steps. I hope this helps. "Schmitz, Nicole" wrote: In our lab we perform RNA in situ hybridizations on paraffin sections. We use the Roche DIG Labelling Kit and the Nucleic Detection Kit (AP-conjugated antibody and NBT/BCIP staining). We tried to optimize the protocol but there are still some problems occuring. After getting rid of the background staining, which took quite a while we now have the problem that after the stopping of the final staining reaction the sections go on turning darker and darker over the next few days. Even the sense probe turns dark and if there is a high mRNA expression the sections sometimes turn so dark that you can see nothing anymore except the dark colour. Has anyone encountered that kind of problem before and has any suggestions? The problem is that we have to store the slides so just taking pictures right after staining is not the best solution.. And I have another question. While trying to solve the problem I found out that some people use an Poly d(T) oligonucleotide to measure RNA degradation in the tissue. Does anyone know more about that e.g. where to order and how to use? Would I have to use another protocol for that than my normal in situ protocol? Thank you for your help Sincerely, Nicole Schmitz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. From chash <@t> reg2.health.nb.ca Fri Apr 15 07:01:07 2005 From: chash <@t> reg2.health.nb.ca (Sharon Chase) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] RCC Antibody Help Message-ID: I am trying to establish a protocol for Vector's renal cell carcinoma antibody. The data sheet states trypsin digestion for 30 minutes. I obtain negative results with trypsin digestion using normal kidney and clear cell carcinoma as controls. I then did HIER retrieval using Biocare's reveal and borg solutions in their decloaker. The normal kidney's proximal tubules stained but the clear cell was patchy. Repeating the run on the following day, using the same reagents and working dilution, I obtained weak results with normal kidney and negative tumor cells. A fresh daily working dilution(1/50), I can obtain constant normal kidney staining but usually negative tumor cells. I tried another tumor control block and leaving the antibody overnight. Normal kidney is fine. Open to any suggestions. Sharon Chase Atlantic Health Science Center Saint John NB Canada From GDawson <@t> dynacaremilwaukee.com Fri Apr 15 07:28:27 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] RCC Antibody Help Message-ID: Sharon, I've recently tried the RCC from Biocare and it works very well. It comes as a pre-dilute so just follow the accompanying instructions and the work-up is extremely easy. My only detraction is that it is an abnormally expen$ive antibody. Good Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Sharon Chase [mailto:chash@reg2.health.nb.ca] Sent: Friday, April 15, 2005 6:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RCC Antibody Help I am trying to establish a protocol for Vector's renal cell carcinoma antibody. The data sheet states trypsin digestion for 30 minutes. I obtain negative results with trypsin digestion using normal kidney and clear cell carcinoma as controls. I then did HIER retrieval using Biocare's reveal and borg solutions in their decloaker. The normal kidney's proximal tubules stained but the clear cell was patchy. Repeating the run on the following day, using the same reagents and working dilution, I obtained weak results with normal kidney and negative tumor cells. A fresh daily working dilution(1/50), I can obtain constant normal kidney staining but usually negative tumor cells. I tried another tumor control block and leaving the antibody overnight. Normal kidney is fine. Open to any suggestions. Sharon Chase Atlantic Health Science Center Saint John NB Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri Apr 15 08:30:15 2005 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Temp/Humidity Records? Message-ID: During a recent inspection, the SOP and documentation for room temperature/humidity in the histo lab were noted as being absent. Although I document instrument temperatures, I have not kept records on room temp/humidity. It was noted that these factors may affect IHC staining quality. If I am to begin documenting these parameters, I'd like to know if there are any guidelines in a general sense (or a specific one) relating to the variances and requirements. Any help would be appreciated! Sara A. Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX From Terry.Marshall <@t> rothgen.nhs.uk Fri Apr 15 08:38:50 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Temp/Humidity Records? Message-ID: So you keep accurate records. What can you do with this information that is actually useful? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] Sent: 15 April 2005 14:30 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Temp/Humidity Records? During a recent inspection, the SOP and documentation for room temperature/humidity in the histo lab were noted as being absent. Although I document instrument temperatures, I have not kept records on room temp/humidity. It was noted that these factors may affect IHC staining quality. If I am to begin documenting these parameters, I'd like to know if there are any guidelines in a general sense (or a specific one) relating to the variances and requirements. Any help would be appreciated! Sara A. Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Fri Apr 15 08:46:10 2005 From: pmarcum <@t> vet.upenn.edu (Pamela A. Marcum) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Temp/Humidity Records? In-Reply-To: References: Message-ID: <1113572770.425fc5a21ff35@imp.vet.upenn.edu> The comment for the records were regarding IHC and yet I find changes in humidity and temperature have more effect on my sectioning and mounting of paraffin and plastic sections. If you are doing automated IHC I don't see how this would matter. If it is manual and you use a humidity chamber it still won't matter much. I could only see this mattering to IHC if it is done on the bench in open air. The changes I get seasons change throughout the day as heat or AC are added or changed. Is this really a new requirement?? Pam Marcum Quoting "Marshall Terry Dr, Consultant Histopathologist" : > So you keep accurate records. > What can you do with this information that is actually useful? > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] > Sent: 15 April 2005 14:30 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temp/Humidity Records? > > > During a recent inspection, the SOP and documentation for room > temperature/humidity in the histo lab were noted as being absent. > Although I document instrument temperatures, I have not kept records on > room temp/humidity. It was noted that these factors may affect IHC > staining quality. If I am to begin documenting these parameters, I'd > like to know if there are any guidelines in a general sense (or a > specific one) relating to the variances and requirements. Any help > would be appreciated! > > Sara A. Breeden, HT(ASCP) > New Mexico Department of Agriculture > Veterinary Diagnostic Services > POBox 4700 > Albuquerque, NM 87196 > (505)841-2576 > (505)841-2518 FAX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cwscouten <@t> myneurolab.com Fri Apr 15 09:45:13 2005 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Long and short biopsy runs with one processor Message-ID: <5784D843593D874C93E9BADCB87342AB44F84E@tpiserver03.Coretech-holdings.com> We offer a processor able to simultaneously process two runs, so you can be doing the long run while ripping through several short runs. It is a dip and dunk system, like a stainer, but has thorough vapor emission control. This style has less carryover and mixing than a single tank systems, and lets more than one run be processing at any one time. The TPC 15 DUO Tissue Processor Product: #511101 from Vibratome, made by Medite TPC 15 DUO Tissue Processor http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=511101&catdesc=Histology+Equipment&CatThreeID=595&CatOneID=4&subcatdesc=Tissue+Processing+Systems&idsubcategory=180 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rush, Joyce Sent: Wednesday, April 13, 2005 12:57 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Long and short biopsy runs with one processor We are a community hospital and last year processed 28,000 blocks. We are being challenged by our Laboratory Director to run a long and short, biopsy run daily, M-F. Currently we run everything together. We have one processor, a VIP5. I'd love to hear of ways people have handled this. Thanks so much! I always get such good advice from this group! Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jo-ann.bader <@t> mcgill.ca Fri Apr 15 09:50:55 2005 From: jo-ann.bader <@t> mcgill.ca (jo-ann-e.bader) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] CD31 on Mouse Message-ID: Good morning everyone, I have a couple of labs in our group who would like to do immuno with CD31 on formalin fixed, paraffin, mouse tissue. ?they cannot seem to find a method that works. Any advise will be appreciated. Thank you in advance Jo-Ann Bader -- Director's Assistant Molecular Oncology Group McGill University Health Centre 687 Pine Ave. W. Room H5-21 Montreal, QC,Canada, H3A-1A1 Tel: 514-843-1479 Fax; 514-843-1478 E-mail: jo-ann.bader@mcgill.ca From liz <@t> premierlab.com Fri Apr 15 10:17:32 2005 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] CD31 on Mouse In-Reply-To: Message-ID: <000201c541ce$424713f0$76d48a80@AMY> Jo-Ann We use the goat polyclonal from santa cruz. Works great on FFPE tissue. I'll send you the protocol and image off the histonet. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jo-ann-e.bader Sent: Friday, April 15, 2005 7:51 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CD31 on Mouse Good morning everyone, I have a couple of labs in our group who would like to do immuno with CD31 on formalin fixed, paraffin, mouse tissue. ?they cannot seem to find a method that works. Any advise will be appreciated. Thank you in advance Jo-Ann Bader -- Director's Assistant Molecular Oncology Group McGill University Health Centre 687 Pine Ave. W. Room H5-21 Montreal, QC,Canada, H3A-1A1 Tel: 514-843-1479 Fax; 514-843-1478 E-mail: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> state.pa.us Fri Apr 15 10:36:29 2005 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Temp/Humidity Records? Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57006C557D@enhbgpri04.pa.lcl> We went thru this same ordeal a few years ago during our AAVLD inspection. I also had the same comments regarding the closed conditions of automated IHC and how room temp and humidity would have little or any affect on IHC. The inspector explained the requirement is justified when troubleshooting any testing that has somehow been transformed over time based on your control changes. The data would be used to rule out the room conditions more then to blame the room conditions as the cause. Our whole laboratory is now wired to monitor these conditions. Roger Charles TSE and IHC Technician Pa Veterinary Laboratory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela A. Marcum Sent: Friday, April 15, 2005 9:46 AM To: Marshall Terry Dr,Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu; Breeden,Sara Subject: RE: [Histonet] Temp/Humidity Records? The comment for the records were regarding IHC and yet I find changes in humidity and temperature have more effect on my sectioning and mounting of paraffin and plastic sections. If you are doing automated IHC I don't see how this would matter. If it is manual and you use a humidity chamber it still won't matter much. I could only see this mattering to IHC if it is done on the bench in open air. The changes I get seasons change throughout the day as heat or AC are added or changed. Is this really a new requirement?? Pam Marcum Quoting "Marshall Terry Dr, Consultant Histopathologist" : > So you keep accurate records. > What can you do with this information that is actually useful? > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] > Sent: 15 April 2005 14:30 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temp/Humidity Records? > > > During a recent inspection, the SOP and documentation for room > temperature/humidity in the histo lab were noted as being absent. > Although I document instrument temperatures, I have not kept records on > room temp/humidity. It was noted that these factors may affect IHC > staining quality. If I am to begin documenting these parameters, I'd > like to know if there are any guidelines in a general sense (or a > specific one) relating to the variances and requirements. Any help > would be appreciated! > > Sara A. Breeden, HT(ASCP) > New Mexico Department of Agriculture > Veterinary Diagnostic Services > POBox 4700 > Albuquerque, NM 87196 > (505)841-2576 > (505)841-2518 FAX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KMH.02 <@t> ex.uchs.org Fri Apr 15 10:42:20 2005 From: KMH.02 <@t> ex.uchs.org (Hopkins, Karen) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] RE: CYTOLOGY PREP BY HISTOLOGY Message-ID: <640B23A5DC4B234BB065E56F2DB30596D962FA@uchex2ucmc.uchs.org> The Histology department at our Hospital is responsible for prepping all cytology specimens. I am interested in information about methods that other facilities use to prep cytology specimens. Generally, we accept direct smears for GYN specimens and send thinprep gyn's out. For non-gyn specimens we do a combination of air-dryed smears, direct smears, thinprep slides, and cytospin slides (Shandon 4 cytospin). We centrifuge many of the specimens and also vortex some. CB's we basically just centrifuge with CytoLyt solution and submit the cell button. I am curious about the following: Filtration methods including cyto-shuttle filtration, Plasma-Thrombin and cytoblock methods for cellblocks, and Autocyte Prep for thin layer preparation systems. Also, if anyone has any knowledge comparing thinprep to Surepath for gyn's I be interested in your comments. I'm working on something for continuing education and want to include the methods I am not familiar with if they are being utilized today. Thanks in advance for any and all responses. Karen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, April 15, 2005 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 17, Issue 25 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: EDTA --> pure water (John Kiernan) 2. Incredible book on mouse anatomy (Gayle Callis) 3. Re: Her2 testing (Promila Rastogi) 4. Source for adjustable workstations? (Tim Webster) 5. RE: Source for adjustable workstations? (Kristen Broomall) 6. Re: Tween charge (Jennifer MacDonald) 7. Source for adjustable workstations? (David Haagsma) 8. "Vision Biosystems is now being distributed in the US" (saya narra) 9. RE: Source for adjustable workstations? (Larry Woody) 10. Timer Search (Mitchell Jean A.) 11. Microm IHC stainer (Carol Bobrowitz) 12. RE: Processors (Bill Sinai) 13. RE: Long and short biopsy runs with one processor (Bill Sinai) 14. Distributor (saya narra) 15. Re: Histonet Posting - Tampa Area (JMyers1@aol.com) 16. RNA in situ hybridization-staining problems (Schmitz, Nicole) 17. Re: RNA in situ hybridization-staining problems (- -) 18. RCC Antibody Help (Sharon Chase) 19. RE: RCC Antibody Help (Dawson, Glen) 20. Temp/Humidity Records? (Breeden, Sara) 21. RE: Temp/Humidity Records? (Marshall Terry Dr, Consultant Histopathologist) 22. RE: Temp/Humidity Records? (Pamela A. Marcum) 23. RE: Long and short biopsy runs with one processor (Charles Scouten) 24. CD31 on Mouse (jo-ann-e.bader) 25. RE: CD31 on Mouse (Elizabeth Chlipala) 26. RE: Temp/Humidity Records? (Charles, Roger) ---------------------------------------------------------------------- Message: 1 Date: Thu, 14 Apr 2005 12:58:24 -0400 From: John Kiernan Subject: Re: [Histonet] EDTA --> pure water To: Trisha Emry Cc: Histonet@lists.utsouthwestern.edu Message-ID: <425EA130.CBBBA9BC@uwo.ca> Content-Type: text/plain; charset=us-ascii When instructions specify "water," without qualification, it is usual to use purified water. If tap water (whatever might be dissolved in it) is OK, the instructions will usually say "tap water" explicitly. EDTA has various uses; in histological work they mostly involve removal of calcium ions. If your tap water is hard (= contains calcium ions), it will use up some of the EDTA in the solution. If you know the Ca content of the tap water you can calculate the equivalent amount of wasted EDTA. One mole of calcium ions (40 grams) combines with one mole of disodium EDTA dihydrate (372 grams). For example, if a litre of tap water contains 100mg of calcium, that will consume 100X372/40 =930mg of EDTA. This would reduce the available EDTA in a 1% solution to about 0.9%. I do not know if my guess of 100ppm Ca is anywhere near reasonable for hard water, or if a certificate of tap water analysis reports the calcium content in this way. If my guesstimate is too low, hard water may eat up more than 10% of the EDTA in a 1% solution. A simple test for minimally "clean" lab water is to mix it with an equal volume of 1% aqueous silver nitrate. Any precipitate or opalescence indicates that the water contains anions with insoluble Ag salts or organic compounds that reduce Ag+. John Kiernan Anatomy, UWO, London, Canada. _______________________________________ Trisha Emry wrote: > > Is it necessary to use distilled H20 for EDTA or can you use tap? > > Is anyone using heat with EDTA? Please send details. > > Thank you, > > Trisha Emry > U of WA, Seattle ________________________________ ------------------------------ Message: 2 Date: Thu, 14 Apr 2005 11:49:30 -0600 From: Gayle Callis Subject: [Histonet] Incredible book on mouse anatomy To: Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050414114731.01b06fd0@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed A Color Atlas of Sectional Anatomy of the Mouse by Takamasa Iwaki, Hiroshi Yamashita, Toshiyuki Hayakawa Published in Japan in 15th September 2001 185 Pages Type: hardcover It costs $175.00, but is filled with spectacular color photos, text is in English and Japanese, Go to Braintree Scientific Website Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 3 Date: Thu, 14 Apr 2005 11:08:30 -0700 From: "Promila Rastogi" Subject: Re: [Histonet] Her2 testing To: , "Cindy DuBois" Message-ID: <37DC9F93CF7F864182D0463EF93D571B034B40@ISLETON2.california.biogenex.com> Content-Type: text/plain; charset="us-ascii" FYI, BioGenex recently received FDA approval for our InSite HER2/neu kit. Promila Rastogi Reagents Product Manager >Message: 17 >Date: Thu, 14 Apr 2005 09:19:51 -0700 (PDT) >From: Cindy DuBois >Subject: [Histonet] Her2 testing >To: Histonet >Message-ID: <20050414161951.64144.qmail@web32107.mail.mud.yahoo.com> >Content-Type: text/plain; charset=us-ascii >Is it still true that only the Dako Her2 is FDA approved? >If not, what everyone's preference for this antibdody? >Cindy DuBois ------------------------------ Message: 4 Date: Thu, 14 Apr 2005 14:17:22 -0400 From: "Tim Webster" Subject: [Histonet] Source for adjustable workstations? To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi All, A recent ergonomic assessment of our Histo Lab produced a seven page litany of ergonomic ills (Yay!) As we are about to renovate in stages, I was looking for a source of adjustable tables/benches/stations for embedding and microtomy. Staff height varies from 5'2" to 6'1+3/4. Adjusting the height of the chair is inadequate for the height of the benches and equipment. I have one supplier of adjustable tables (Custom-products.com) I would love someone to offer another name. (I struggle finding stuff on the web sometimes . . .) Also, how do you folks handle ergonomics for routine histo? Have a great day Tim Tim Webster Histology Specialist Northwestern Medical Center 133 Fairfield Street St Albans, VT 05478 (802) 524-1070 twebster@nmcinc.org ------------------------------ Message: 5 Date: Thu, 14 Apr 2005 14:29:44 -0400 From: Kristen Broomall Subject: RE: [Histonet] Source for adjustable workstations? To: 'Tim Webster' , histonet@lists.utsouthwestern.edu Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A79B5@wlmmsx01.nemours.org> Content-Type: text/plain; charset="iso-8859-1" "Also, how do you folks handle ergonomics for routine histo?" Ibuprofen and muscle relaxers. Sorry, I couldn't resist. (I'm not making it up though!) But seriously, I'm 5'2" & get horrible muscle spasms in my shoulder from sitting at the microtome for long periods at a time, no matter how high the chair is positioned. I must stretch my body in some funky direction to reach the wheel. I've gotten used to using the automated part of the microtome with the foot pedal. I'd love to hear what you come up with! Kristen Broomall -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tim Webster Sent: Thursday, April 14, 2005 2:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Source for adjustable workstations? Hi All, A recent ergonomic assessment of our Histo Lab produced a seven page litany of ergonomic ills (Yay!) As we are about to renovate in stages, I was looking for a source of adjustable tables/benches/stations for embedding and microtomy. Staff height varies from 5'2" to 6'1+3/4. Adjusting the height of the chair is inadequate for the height of the benches and equipment. I have one supplier of adjustable tables (Custom-products.com) I would love someone to offer another name. (I struggle finding stuff on the web sometimes . . .) Also, how do you folks handle ergonomics for routine histo? Have a great day Tim Tim Webster Histology Specialist Northwestern Medical Center 133 Fairfield Street St Albans, VT 05478 (802) 524-1070 twebster@nmcinc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 14 Apr 2005 11:34:28 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Tween charge To: Dorothy.L.Webb@HealthPartners.Com Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I think this would fall under the category of soaking, like overdehydrated blocks. There is no charge for this. Dorothy.L.Webb@HealthPartners.Com Sent by: histonet-bounces@lists.utsouthwestern.edu 04/14/2005 07:30 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Tween charge Does anyone know of the "charge" that should be used for softening toe or fingernails to prepare for processing? Could a decal charge be safely used? It seems to me that with taking the time and the solutions involved, there should be some sort of charge incurred. Any thoughts and thanks much!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 14 Apr 2005 14:43:23 -0400 From: "David Haagsma" Subject: [Histonet] Source for adjustable workstations? To: , "Tim Webster" Message-ID: <19E3602A16438E48B51A4250CA04B5F60F98CC@exchange.marketlab.com> Content-Type: text/plain; charset="US-ASCII" Tim, MarketLab Inc. has adjustable microtome stations, http://www.marketlabinc.com/products/product.cfm/ML3158 And adjustable height grossing stations http://www.marketlabinc.com/products/product.cfm/ML3217 http://www.marketlabinc.com/products/categorydetail.cfm/722 And Microtome Arm Rests http://www.marketlabinc.com/products/product.cfm/ML1038L Thank you, Dave Haagsma www.marketlabinc.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Webster Sent: Thursday, April 14, 2005 2:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Source for adjustable workstations? Hi All, A recent ergonomic assessment of our Histo Lab produced a seven page litany of ergonomic ills (Yay!) As we are about to renovate in stages, I was looking for a source of adjustable tables/benches/stations for embedding and microtomy. Staff height varies from 5'2" to 6'1+3/4. Adjusting the height of the chair is inadequate for the height of the benches and equipment. I have one supplier of adjustable tables (Custom-products.com) I would love someone to offer another name. (I struggle finding stuff on the web sometimes . . .) Also, how do you folks handle ergonomics for routine histo? Have a great day Tim Tim Webster Histology Specialist Northwestern Medical Center 133 Fairfield Street St Albans, VT 05478 (802) 524-1070 twebster@nmcinc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 14 Apr 2005 11:46:26 -0700 (PDT) From: saya narra Subject: [Histonet] "Vision Biosystems is now being distributed in the US" To: histonet@lists.utsouthwestern.edu Message-ID: <20050414184626.52381.qmail@web60620.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi Rick ! It appears your automated immunos are overpriced compared to Dako. As a general rule, it's not good to advertise what isn't competitive in a given market. Business 101. Also, you said " Vision Biosystems is now being distributed in the U.S. ". Who is the distributor ? Can you get me the Company info so I may contact them re pricing, service etc. I wasn't aware that Vision Biosystems had found a "distributor". What is the website of the Distributor ? Is it Leica ? Saya Narra UofT __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ ------------------------------ Message: 9 Date: Thu, 14 Apr 2005 11:49:58 -0700 (PDT) From: Larry Woody Subject: RE: [Histonet] Source for adjustable workstations? To: histonet@lists.utsouthwestern.edu Message-ID: <20050414184959.12724.qmail@web52606.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii I have degenerative disc disease in my neck from 25 yrs of cutting but what may surprise you is that standing up to cut bothers me a whole lot less than sitting down, and it is less pressure on the back to stand up and I use a lot less ibuprofen. Larry --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! ------------------------------ Message: 10 Date: Thu, 14 Apr 2005 14:29:14 -0500 From: "Mitchell Jean A." Subject: [Histonet] Timer Search To: Message-ID: <583D3E9A1E843445BD54E461D1A2F6F30F83F596@uwhis-xchng2.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" I am on a search for the large (approx 3 1/2 in square) grey winding (120 minute) timers that were ever so popular in the lab once upon a time. Anyone have a clue if they are still available anywhere? I've been searching for awhile & can't come up with a source. Thanks much, Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Department of Neurology, Neuromuscular Laboratory Madison, WI ------------------------------ Message: 11 Date: Thu, 14 Apr 2005 15:30:39 -0500 From: Carol Bobrowitz Subject: [Histonet] Microm IHC stainer To: "'Histonet (E-mail)" Message-ID: <59DF6640CDC0D7119DF000D0B761393C1A965A@thor.phys.mcw.edu> Content-Type: text/plain; charset="iso-8859-1" I'm curious. Any Histology techs out there that have demo'd the Microm IHC stainer? What are your comments? If Richard Allan was purchased by Fisher, who does the repairs and is there a yearly service contract? Vendors, Please do not send me any information. I would like comments from users not a sales pitch. Thanks, Carol Ann Bobrowitz Histology Laboratory Department of Physiology - Room 541 Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, Wisconsin 53226 414-456-8179 FAX 414-456-6546 ------------------------------ Message: 12 Date: Fri, 15 Apr 2005 07:24:48 +1000 From: "Bill Sinai" Subject: [Histonet] RE: Processors To: "'saya narra'" , "histonet \(E-mail\)" Message-ID: <001401c54138$632f85c0$0ecd080a@wsahs.nsw.gov.au> Content-Type: text/plain; charset="US-ASCII" I believe they are looking for a distributor in the USA. I have heard no rumour about the company being for sale. Histology equipment is but a small part of their whole business. Mt Waverley is in Victoria (the smallest state in Australia) I am in the outer suburbs of Sydney in NSW. The distance between is 1000klm (700miles) Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: saya narra [mailto:sayanarra@yahoo.com] Sent: Thursday, 14 April 2005 7:43 PM To: histonet@lists.utsouthwestern.edu Cc: bills@icpmr.wsahs.nsw.gov.au Subject: Processors Hello Bill, You said your VIP was reliable . I agree, we have one too and it is a good instrument. It seems no one may get a chance to get a Peloris as the competition says Vision Biosystems is FOR SALE or looking for a distributor in the USA. You are in Australia and the Histology field. Thie website says they are in Mt.Waverly, VIC, Australia. Is that near you? Have you heard any word on their Boss going door to door looking for a buyer in the USA ? Saya Narra UofT __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. ------------------------------ Message: 13 Date: Fri, 15 Apr 2005 07:35:31 +1000 From: "Bill Sinai" Subject: RE: [Histonet] Long and short biopsy runs with one processor To: , "histonet \(E-mail\)" Message-ID: <001501c54139$e27d9730$0ecd080a@wsahs.nsw.gov.au> Content-Type: text/plain; charset="US-ASCII" We occasionally run cell blocks but most times they are put through the routine overnight processing. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: dahmed@mdanderson.org [mailto:dahmed@mdanderson.org] Sent: Thursday, 14 April 2005 11:13 PM To: Bill Sinai Subject: RE: [Histonet] Long and short biopsy runs with one processor What about cell blocks? Do you feel that your currenct processing schedule for biopsies would be appropriate for cytology cell blocks? Our current bx/cell block protocol is 20 minutes in all stations including formalin for a total run time of 6 hours? Can I trim this back a little? David S. Ahmed Chief Histology Technician Department of Pathology M.D. Anderson Cancer Center "Bill Sinai" To: Sent by: "'Roxanne Soto'" , "histonet (E-mail)" histonet-bounces@lists.utsouth western.edu cc: 04/13/2005 04:55 PM Subject: RE: [Histonet] Long and short biopsy runs with one processor Roxanne, Most are the small GE biopsies >3mm in diamater or "thin" slices of any urgents >3mm thick. Solution Time Formalin 0min 70%ethanol 5min 95% 5min 100% 5min 100% 10min 100% 10min Xylol 5min Xylol 5min Xylol 10min Wax 5min Wax 10min Wax 10min Temperature ambient with Pressur/Vacuum on all stations. Two Leica ASP300 and two VIP 3000 Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: Roxanne Soto [mailto:godsgirlnow@MSN.COM] Sent: Thursday, 14 April 2005 7:37 AM To: Bill Sinai Subject: Re: [Histonet] Long and short biopsy runs with one processor What size is the tissue on the 2 hour run? How long is each station? What kind of processor? Do you use the vacuum? Roxanne ----- Original Message ----- From: Bill Sinai To: histonet (E-mail) Sent: Wednesday, April 13, 2005 5:30 PM Subject: RE: [Histonet] Long and short biopsy runs with one processor Julia, I agree with your philosophy about small runs. We are a pathology department supporting a large teaching hospital and an even larger area pathology service. However, we also do a considerable amount of private work for several endoscopy clinics in our locality. The scenario you describe is very similar to ours. We have the usual overnight run, usually 450-600 blocks with the large material in one processor and the smaller in another. We receive the endoscopy specimens anytime from 7:30am through to 3:00pm each day, with any pick ups after 6:00pm being processed the next morning in a short run 2-2.5hrs. This means we can have at least two runs per day of endoscopy specimens. The endoscopy specimens from the private clinics are about 30% of our work and the TAT for these specimens is >2 days from pick-up to hard copy result to the clinician. We also process renal biopsies several times per day as well as the occasional urgent specimen. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Julia Dahl Sent: Thursday, 14 April 2005 4:13 AM To: Joyce.Rush@sjmcmn.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Long and short biopsy runs with one processor Joyce - First let me preface with my bias - I am a GI pathologist (read small biopsy material is what I live and breathe). Long processor runs are great for standard surgical material - but absolutely overprocess small pieces of tissue, resulting in hard, dehydrated, difficult to cut little brittle fragments. The best approach that I've seen and used is to examine your "bottlenecks." The main bottlenecks are the points at which you have lots to do in front of you - with limited resource to do it (i.e. you have 150 cassettes to embed coming off of the processor at one time and ONE embedding station. That's a bottleneck.) What time do you usually start your processor? Say your processor starts at 10:00 p.m. with a standard 6 hour process run. Your pathologists or your PAs close the grossing stations at 6:00 p.m. and load the processor - and everything sits there for 4 hours (that's another bottleneck). In this scenario - you can add a second processor run at virtually any time that you are staffed that allows you to turn over the processor for the standard run by 6:00 p.m. (when the standard next load is). Since a small biopsy run usually takes about 2 hours on the Vitek: 0600 - Standard processor emptied - large surgical cases ONLY (about 2/3 of your blocks) 0600 - 1000: Embed, cut, stain and coverslip surgicals 0700 - 0800: Previous day's and early AM courier run of small biopsies grossed in 0800 - 1000: Small biopsy processor run 1000 - small biopsy processor emptied 1000 - 1230: Embed, cut, stain and coverslip biopsies. If you wanted to do another biopsy run - to allow same day turnaround time - the 2nd biopsy run could be scheduled at 10:30 each day with slides coming out around 3:30. I'm interested to hear other people's ideas. Julia >From: "Rush, Joyce" >To: >Subject: [Histonet] Long and short biopsy runs with one processor >Date: Wed, 13 Apr 2005 12:57:07 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by mc7-f23.hotmail.com >with Microsoft SMTPSVC(6.0.3790.211); Wed, 13 Apr 2005 10:58:11 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1DLm7H-0004v5-7P; Wed, 13 Apr >2005 12:57:36 -0500 >Received: from [199.165.152.166] (helo=swlx166.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1DLm6v-0004uy-Kqfor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 12:57:19 -0500 >Received: from [66.173.44.122] (helo=keys.sjmcmn.org)by swlx166.swmed.edu >with esmtp (Exim 4.44) id 1DLm6u-0004b0-5Ffor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 12:57:09 -0500 >Received: from keys.sjmcmn.org (unknown [127.0.0.1])by keys.sjmcmn.org >(Postfix) with ESMTP id 50777256FE9for >;Wed, 13 Apr 2005 12:57:07 -0500 (CDT) >Received: from mail.SJMCMN.ORG ([10.1.0.6])by keys.sjmcmn.org (PGP >Universal service);Wed, 13 Apr 2005 12:57:07 -0500 >X-Message-Info: cmh8WMAfDXqeqGy5Qdhsq/iJQn/CwXc1jm673ZQpEo0= >X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 >Content-class: urn:content-classes:message >X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: Long and short biopsy >runs with one processor >Thread-Index: AcVAUjUG93SXzh8HQoGO/FSJK5JRgA== >X-Scan-Signature: 5ce762e8aa474ea7aaa37532c083de4e >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >,< mailto:histone t-request@lists.utsouthwestern.edu?subject=subscribe> >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 201832d891fa504721b2e650a5a52212 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 13 Apr 2005 17:58:11.0794 (UTC) >FILETIME=[5B956320:01C54052] > >We are a community hospital and last year processed 28,000 blocks. We are >being challenged by our Laboratory Director to run a long and short, biopsy >run daily, M-F. Currently we run everything together. We have one >processor, a VIP5. I'd love to hear of ways people have handled this. >Thanks so much! I always get such good advice from this group! > >Joyce > >Joyce A. Rush, BS, MT(ASCP) >Laboratory Manager >St Joseph's Medical Center >523 North Third Street >Brainerd, MN 56401 >Office:218-828-7500 Fax: 218-828-7510 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. ------------------------------ Message: 14 Date: Thu, 14 Apr 2005 18:20:11 -0700 (PDT) From: saya narra Subject: [Histonet] Distributor To: histonet@lists.utsouthwestern.edu Message-ID: <20050415012011.27372.qmail@web60617.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Rick Couture, Thank you for the information. I will speak with our Lab Manager and have her contact the Distributor of the Vision Biosystem Instruments directly, should we decide on the need to purchase. Who was the Distributor you spoke of again ? saya narra UofT __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ ------------------------------ Message: 15 Date: Fri, 15 Apr 2005 00:48:07 EDT From: JMyers1@aol.com Subject: [Histonet] Re: Histonet Posting - Tampa Area To: histonet@lists.utsouthwestern.edu Message-ID: <1ab.36836df6.2f90a187@aol.com> Content-Type: text/plain; charset="US-ASCII" Carol: I am quite familiar with this institution; I worked as the lab manager in another hospital that's just a few miles away, and I've known several of the techs who have worked here. Please feel free to call/write me... Joe Myers, M.S., CT(ASCP) Clearwater, FL 727-504-0250 ******************************** Message: 8 Date: Wed, 13 Apr 2005 14:09:01 -0500 From: cjohnston@mdanderson.org Subject: [Histonet] Tampa Area To: histonet@pathology.swmed.edu I am looking for anyone familiar with Sun Coast Hospital in the Tampa area. If you could email me privately, I would appreciate it . Carol M. Johnston HT(ASCP) Division of Surgery M.D. Anderson Cancer Center 1515 Holcombe Blvd. Unit 017 Houston, Texas 77030 713-745-4625 fax: 713-745-2548 ------------------------------ Message: 16 Date: Fri, 15 Apr 2005 09:54:26 +0200 From: "Schmitz, Nicole" Subject: [Histonet] RNA in situ hybridization-staining problems To: Message-ID: <320709412D172C48A73DA1D2D0BE20820184AFD1@UKMSRV032.ukmuenster.de> Content-Type: text/plain; charset="iso-8859-1" In our lab we perform RNA in situ hybridizations on paraffin sections. We use the Roche DIG Labelling Kit and the Nucleic Detection Kit (AP-conjugated antibody and NBT/BCIP staining). We tried to optimize the protocol but there are still some problems occuring. After getting rid of the background staining, which took quite a while we now have the problem that after the stopping of the final staining reaction the sections go on turning darker and darker over the next few days. Even the sense probe turns dark and if there is a high mRNA expression the sections sometimes turn so dark that you can see nothing anymore except the dark colour. Has anyone encountered that kind of problem before and has any suggestions? The problem is that we have to store the slides so just taking pictures right after staining is not the best solution.. And I have another question. While trying to solve the problem I found out that some people use an Poly d(T) oligonucleotide to measure RNA degradation in the tissue. Does anyone know more about that e.g. where to order and how to use? Would I have to use another protocol for that than my normal in situ protocol? Thank you for your help Sincerely, Nicole Schmitz ------------------------------ Message: 17 Date: Fri, 15 Apr 2005 03:42:50 -0700 (PDT) From: - - Subject: Re: [Histonet] RNA in situ hybridization-staining problems To: histonet@lists.utsouthwestern.edu Message-ID: <20050415104250.78402.qmail@web42302.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Nicole, I encountered the same problem and switched to the HRP-conjugated anti-DIG antibody also by Roche. I used a TSA amplification system (Biogenex - mainly used for their machines, but I was able to use it by hand also) and finally, detected with DAB. All my samples come out great! There was need for some adjustment as I was getting background initially. But I got rid of that with time adjustment and blocking steps. I hope this helps. "Schmitz, Nicole" wrote: In our lab we perform RNA in situ hybridizations on paraffin sections. We use the Roche DIG Labelling Kit and the Nucleic Detection Kit (AP-conjugated antibody and NBT/BCIP staining). We tried to optimize the protocol but there are still some problems occuring. After getting rid of the background staining, which took quite a while we now have the problem that after the stopping of the final staining reaction the sections go on turning darker and darker over the next few days. Even the sense probe turns dark and if there is a high mRNA expression the sections sometimes turn so dark that you can see nothing anymore except the dark colour. Has anyone encountered that kind of problem before and has any suggestions? The problem is that we have to store the slides so just taking pictures right after staining is not the best solution.. And I have another question. While trying to solve the problem I found out that some people use an Poly d(T) oligonucleotide to measure RNA degradation in the tissue. Does anyone know more about that e.g. where to order and how to use? Would I have to use another protocol for that than my normal in situ protocol? Thank you for your help Sincerely, Nicole Schmitz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. ------------------------------ Message: 18 Date: Fri, 15 Apr 2005 09:01:07 -0300 From: "Sharon Chase" Subject: [Histonet] RCC Antibody Help To: Message-ID: Content-Type: text/plain; charset=US-ASCII I am trying to establish a protocol for Vector's renal cell carcinoma antibody. The data sheet states trypsin digestion for 30 minutes. I obtain negative results with trypsin digestion using normal kidney and clear cell carcinoma as controls. I then did HIER retrieval using Biocare's reveal and borg solutions in their decloaker. The normal kidney's proximal tubules stained but the clear cell was patchy. Repeating the run on the following day, using the same reagents and working dilution, I obtained weak results with normal kidney and negative tumor cells. A fresh daily working dilution(1/50), I can obtain constant normal kidney staining but usually negative tumor cells. I tried another tumor control block and leaving the antibody overnight. Normal kidney is fine. Open to any suggestions. Sharon Chase Atlantic Health Science Center Saint John NB Canada ------------------------------ Message: 19 Date: Fri, 15 Apr 2005 07:28:27 -0500 From: "Dawson, Glen" Subject: RE: [Histonet] RCC Antibody Help To: Sharon Chase , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Sharon, I've recently tried the RCC from Biocare and it works very well. It comes as a pre-dilute so just follow the accompanying instructions and the work-up is extremely easy. My only detraction is that it is an abnormally expen$ive antibody. Good Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Sharon Chase [mailto:chash@reg2.health.nb.ca] Sent: Friday, April 15, 2005 6:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RCC Antibody Help I am trying to establish a protocol for Vector's renal cell carcinoma antibody. The data sheet states trypsin digestion for 30 minutes. I obtain negative results with trypsin digestion using normal kidney and clear cell carcinoma as controls. I then did HIER retrieval using Biocare's reveal and borg solutions in their decloaker. The normal kidney's proximal tubules stained but the clear cell was patchy. Repeating the run on the following day, using the same reagents and working dilution, I obtained weak results with normal kidney and negative tumor cells. A fresh daily working dilution(1/50), I can obtain constant normal kidney staining but usually negative tumor cells. I tried another tumor control block and leaving the antibody overnight. Normal kidney is fine. Open to any suggestions. Sharon Chase Atlantic Health Science Center Saint John NB Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Fri, 15 Apr 2005 07:30:15 -0600 From: "Breeden, Sara" Subject: [Histonet] Temp/Humidity Records? To: Message-ID: Content-Type: text/plain; charset="us-ascii" During a recent inspection, the SOP and documentation for room temperature/humidity in the histo lab were noted as being absent. Although I document instrument temperatures, I have not kept records on room temp/humidity. It was noted that these factors may affect IHC staining quality. If I am to begin documenting these parameters, I'd like to know if there are any guidelines in a general sense (or a specific one) relating to the variances and requirements. Any help would be appreciated! Sara A. Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX ------------------------------ Message: 21 Date: Fri, 15 Apr 2005 14:38:50 +0100 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] Temp/Humidity Records? To: "Breeden, Sara" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" So you keep accurate records. What can you do with this information that is actually useful? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] Sent: 15 April 2005 14:30 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Temp/Humidity Records? During a recent inspection, the SOP and documentation for room temperature/humidity in the histo lab were noted as being absent. Although I document instrument temperatures, I have not kept records on room temp/humidity. It was noted that these factors may affect IHC staining quality. If I am to begin documenting these parameters, I'd like to know if there are any guidelines in a general sense (or a specific one) relating to the variances and requirements. Any help would be appreciated! Sara A. Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Fri, 15 Apr 2005 09:46:10 -0400 From: "Pamela A. Marcum" Subject: RE: [Histonet] Temp/Humidity Records? To: "Marshall Terry Dr, Consultant Histopathologist" Cc: histonet@lists.utsouthwestern.edu, "Breeden, Sara" Message-ID: <1113572770.425fc5a21ff35@imp.vet.upenn.edu> Content-Type: text/plain; charset=ISO-8859-1 The comment for the records were regarding IHC and yet I find changes in humidity and temperature have more effect on my sectioning and mounting of paraffin and plastic sections. If you are doing automated IHC I don't see how this would matter. If it is manual and you use a humidity chamber it still won't matter much. I could only see this mattering to IHC if it is done on the bench in open air. The changes I get seasons change throughout the day as heat or AC are added or changed. Is this really a new requirement?? Pam Marcum Quoting "Marshall Terry Dr, Consultant Histopathologist" : > So you keep accurate records. > What can you do with this information that is actually useful? > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] > Sent: 15 April 2005 14:30 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temp/Humidity Records? > > > During a recent inspection, the SOP and documentation for room > temperature/humidity in the histo lab were noted as being absent. > Although I document instrument temperatures, I have not kept records on > room temp/humidity. It was noted that these factors may affect IHC > staining quality. If I am to begin documenting these parameters, I'd > like to know if there are any guidelines in a general sense (or a > specific one) relating to the variances and requirements. Any help > would be appreciated! > > Sara A. Breeden, HT(ASCP) > New Mexico Department of Agriculture > Veterinary Diagnostic Services > POBox 4700 > Albuquerque, NM 87196 > (505)841-2576 > (505)841-2518 FAX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 23 Date: Fri, 15 Apr 2005 09:45:13 -0500 From: "Charles Scouten" Subject: RE: [Histonet] Long and short biopsy runs with one processor To: "Rush, Joyce" , Message-ID: <5784D843593D874C93E9BADCB87342AB44F84E@tpiserver03.Coretech-holdings.com> Content-Type: text/plain; charset="iso-8859-1" We offer a processor able to simultaneously process two runs, so you can be doing the long run while ripping through several short runs. It is a dip and dunk system, like a stainer, but has thorough vapor emission control. This style has less carryover and mixing than a single tank systems, and lets more than one run be processing at any one time. The TPC 15 DUO Tissue Processor Product: #511101 from Vibratome, made by Medite TPC 15 DUO Tissue Processor http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=511101&catdesc=Histology+Equipment&CatThreeID=595&CatOneID=4&subcatdesc=Tissue+Processing+Systems&idsubcategory=180 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rush, Joyce Sent: Wednesday, April 13, 2005 12:57 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Long and short biopsy runs with one processor We are a community hospital and last year processed 28,000 blocks. We are being challenged by our Laboratory Director to run a long and short, biopsy run daily, M-F. Currently we run everything together. We have one processor, a VIP5. I'd love to hear of ways people have handled this. Thanks so much! I always get such good advice from this group! Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 24 Date: Fri, 15 Apr 2005 10:50:55 -0400 From: "jo-ann-e.bader" Subject: [Histonet] CD31 on Mouse To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Good morning everyone, I have a couple of labs in our group who would like to do immuno with CD31 on formalin fixed, paraffin, mouse tissue. ?they cannot seem to find a method that works. Any advise will be appreciated. Thank you in advance Jo-Ann Bader -- Director's Assistant Molecular Oncology Group McGill University Health Centre 687 Pine Ave. W. Room H5-21 Montreal, QC,Canada, H3A-1A1 Tel: 514-843-1479 Fax; 514-843-1478 E-mail: jo-ann.bader@mcgill.ca ------------------------------ Message: 25 Date: Fri, 15 Apr 2005 09:17:32 -0600 From: "Elizabeth Chlipala" Subject: RE: [Histonet] CD31 on Mouse To: "'jo-ann-e.bader'" , "'Histonet'" Message-ID: <000201c541ce$424713f0$76d48a80@AMY> Content-Type: text/plain; charset="US-ASCII" Jo-Ann We use the goat polyclonal from santa cruz. Works great on FFPE tissue. I'll send you the protocol and image off the histonet. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jo-ann-e.bader Sent: Friday, April 15, 2005 7:51 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CD31 on Mouse Good morning everyone, I have a couple of labs in our group who would like to do immuno with CD31 on formalin fixed, paraffin, mouse tissue. ?they cannot seem to find a method that works. Any advise will be appreciated. Thank you in advance Jo-Ann Bader -- Director's Assistant Molecular Oncology Group McGill University Health Centre 687 Pine Ave. W. Room H5-21 Montreal, QC,Canada, H3A-1A1 Tel: 514-843-1479 Fax; 514-843-1478 E-mail: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 26 Date: Fri, 15 Apr 2005 11:36:29 -0400 From: "Charles, Roger" Subject: RE: [Histonet] Temp/Humidity Records? To: Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57006C557D@enhbgpri04.pa.lcl> Content-Type: text/plain; charset="us-ascii" We went thru this same ordeal a few years ago during our AAVLD inspection. I also had the same comments regarding the closed conditions of automated IHC and how room temp and humidity would have little or any affect on IHC. The inspector explained the requirement is justified when troubleshooting any testing that has somehow been transformed over time based on your control changes. The data would be used to rule out the room conditions more then to blame the room conditions as the cause. Our whole laboratory is now wired to monitor these conditions. Roger Charles TSE and IHC Technician Pa Veterinary Laboratory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela A. Marcum Sent: Friday, April 15, 2005 9:46 AM To: Marshall Terry Dr,Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu; Breeden,Sara Subject: RE: [Histonet] Temp/Humidity Records? The comment for the records were regarding IHC and yet I find changes in humidity and temperature have more effect on my sectioning and mounting of paraffin and plastic sections. If you are doing automated IHC I don't see how this would matter. If it is manual and you use a humidity chamber it still won't matter much. I could only see this mattering to IHC if it is done on the bench in open air. The changes I get seasons change throughout the day as heat or AC are added or changed. Is this really a new requirement?? Pam Marcum Quoting "Marshall Terry Dr, Consultant Histopathologist" : > So you keep accurate records. > What can you do with this information that is actually useful? > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] > Sent: 15 April 2005 14:30 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temp/Humidity Records? > > > During a recent inspection, the SOP and documentation for room > temperature/humidity in the histo lab were noted as being absent. > Although I document instrument temperatures, I have not kept records on > room temp/humidity. It was noted that these factors may affect IHC > staining quality. If I am to begin documenting these parameters, I'd > like to know if there are any guidelines in a general sense (or a > specific one) relating to the variances and requirements. Any help > would be appreciated! > > Sara A. Breeden, HT(ASCP) > New Mexico Department of Agriculture > Veterinary Diagnostic Services > POBox 4700 > Albuquerque, NM 87196 > (505)841-2576 > (505)841-2518 FAX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 17, Issue 25 **************************************** From Terry.Marshall <@t> rothgen.nhs.uk Fri Apr 15 10:44:50 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Temp/Humidity Records? Message-ID: This differs from being a waste of time and effort in what way? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Charles, Roger [mailto:rcharles@state.pa.us] Sent: 15 April 2005 16:36 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? We went thru this same ordeal a few years ago during our AAVLD inspection. I also had the same comments regarding the closed conditions of automated IHC and how room temp and humidity would have little or any affect on IHC. The inspector explained the requirement is justified when troubleshooting any testing that has somehow been transformed over time based on your control changes. The data would be used to rule out the room conditions more then to blame the room conditions as the cause. Our whole laboratory is now wired to monitor these conditions. Roger Charles TSE and IHC Technician Pa Veterinary Laboratory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela A. Marcum Sent: Friday, April 15, 2005 9:46 AM To: Marshall Terry Dr,Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu; Breeden,Sara Subject: RE: [Histonet] Temp/Humidity Records? The comment for the records were regarding IHC and yet I find changes in humidity and temperature have more effect on my sectioning and mounting of paraffin and plastic sections. If you are doing automated IHC I don't see how this would matter. If it is manual and you use a humidity chamber it still won't matter much. I could only see this mattering to IHC if it is done on the bench in open air. The changes I get seasons change throughout the day as heat or AC are added or changed. Is this really a new requirement?? Pam Marcum Quoting "Marshall Terry Dr, Consultant Histopathologist" : > So you keep accurate records. > What can you do with this information that is actually useful? > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] > Sent: 15 April 2005 14:30 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temp/Humidity Records? > > > During a recent inspection, the SOP and documentation for room > temperature/humidity in the histo lab were noted as being absent. > Although I document instrument temperatures, I have not kept records on > room temp/humidity. It was noted that these factors may affect IHC > staining quality. If I am to begin documenting these parameters, I'd > like to know if there are any guidelines in a general sense (or a > specific one) relating to the variances and requirements. Any help > would be appreciated! > > Sara A. Breeden, HT(ASCP) > New Mexico Department of Agriculture > Veterinary Diagnostic Services > POBox 4700 > Albuquerque, NM 87196 > (505)841-2576 > (505)841-2518 FAX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ajennings <@t> unmc.edu Fri Apr 15 11:37:36 2005 From: ajennings <@t> unmc.edu (Anita Jennings) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Tape transfer sections and IHC In-Reply-To: <200504121611.j3CGB1OD014811@chip.viawest.net> Message-ID: I had problems with a 'matrix' looking background and Bernice said they were working on a special mounting media. I know she checks the histonet so hopefully she will answer here. I am doing hrp IHC on tape transfer sections and I am experiencing a problem with unwanted staining on the coating on the polymer coated slides. Does anyone else out there do IHC on the tape sections experience this problem? Please suggest a solution to this. Thank you, Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ajennings <@t> unmc.edu Fri Apr 15 11:42:13 2005 From: ajennings <@t> unmc.edu (Anita Jennings) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Timer Search In-Reply-To: <583D3E9A1E843445BD54E461D1A2F6F30F83F596@uwhis-xchng2.hosp.wisc.edu> Message-ID: Your best bet would probably be a photo supply store or maybe Carolina Biologicals use to carry "school" supply I remember taking many a lab practical watching one of those clocks tick down as you moved from station to station "Mitchell Jean A." Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Timer Search 04/14/2005 02:29 PM I am on a search for the large (approx 3 1/2 in square) grey winding (120 minute) timers that were ever so popular in the lab once upon a time. Anyone have a clue if they are still available anywhere? I've been searching for awhile & can't come up with a source. Thanks much, Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Department of Neurology, Neuromuscular Laboratory Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> state.pa.us Fri Apr 15 11:54:12 2005 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Temp/Humidity Records? Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57006C557F@enhbgpri04.pa.lcl> This differs in being an accredited lab vs a non-accredited lab. -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Friday, April 15, 2005 11:45 AM To: Charles, Roger; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? This differs from being a waste of time and effort in what way? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Charles, Roger [mailto:rcharles@state.pa.us] Sent: 15 April 2005 16:36 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? We went thru this same ordeal a few years ago during our AAVLD inspection. I also had the same comments regarding the closed conditions of automated IHC and how room temp and humidity would have little or any affect on IHC. The inspector explained the requirement is justified when troubleshooting any testing that has somehow been transformed over time based on your control changes. The data would be used to rule out the room conditions more then to blame the room conditions as the cause. Our whole laboratory is now wired to monitor these conditions. Roger Charles TSE and IHC Technician Pa Veterinary Laboratory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela A. Marcum Sent: Friday, April 15, 2005 9:46 AM To: Marshall Terry Dr,Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu; Breeden,Sara Subject: RE: [Histonet] Temp/Humidity Records? The comment for the records were regarding IHC and yet I find changes in humidity and temperature have more effect on my sectioning and mounting of paraffin and plastic sections. If you are doing automated IHC I don't see how this would matter. If it is manual and you use a humidity chamber it still won't matter much. I could only see this mattering to IHC if it is done on the bench in open air. The changes I get seasons change throughout the day as heat or AC are added or changed. Is this really a new requirement?? Pam Marcum Quoting "Marshall Terry Dr, Consultant Histopathologist" : > So you keep accurate records. > What can you do with this information that is actually useful? > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] > Sent: 15 April 2005 14:30 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temp/Humidity Records? > > > During a recent inspection, the SOP and documentation for room > temperature/humidity in the histo lab were noted as being absent. > Although I document instrument temperatures, I have not kept records on > room temp/humidity. It was noted that these factors may affect IHC > staining quality. If I am to begin documenting these parameters, I'd > like to know if there are any guidelines in a general sense (or a > specific one) relating to the variances and requirements. Any help > would be appreciated! > > Sara A. Breeden, HT(ASCP) > New Mexico Department of Agriculture > Veterinary Diagnostic Services > POBox 4700 > Albuquerque, NM 87196 > (505)841-2576 > (505)841-2518 FAX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Fri Apr 15 12:09:46 2005 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] RCC Antibody Help Message-ID: We used to use Novocastra's RCC mAb, but were unhappy with its performance. Try Lab Vision's (www.labvision.com) mAb; I think you will be satisfied. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Sharon Chase" 04/15/05 08:01AM >>> I am trying to establish a protocol for Vector's renal cell carcinoma antibody. The data sheet states trypsin digestion for 30 minutes. I obtain negative results with trypsin digestion using normal kidney and clear cell carcinoma as controls. I then did HIER retrieval using Biocare's reveal and borg solutions in their decloaker. The normal kidney's proximal tubules stained but the clear cell was patchy. Repeating the run on the following day, using the same reagents and working dilution, I obtained weak results with normal kidney and negative tumor cells. A fresh daily working dilution(1/50), I can obtain constant normal kidney staining but usually negative tumor cells. I tried another tumor control block and leaving the antibody overnight. Normal kidney is fine. Open to any suggestions. Sharon Chase Atlantic Health Science Center Saint John NB Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------- Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From machadok <@t> drhsi.org Fri Apr 15 12:18:52 2005 From: machadok <@t> drhsi.org (Kathy Machado) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Looking for someone Message-ID: <7C1D5BE1ADF06E488AEB7A6247214A8682B2EF@HORNET.drmc.drhsi.org> Hello all, I am looking for Barbara Vissar. Last I knew she had left Colorado for Michigan. If anyone knows how to contact her please let me know. Barb, it has been five years... Sincerely, Kathy Machado Danville Regional Medical Center Danville,Virgina From gcallis <@t> montana.edu Fri Apr 15 12:19:41 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Tape transfer sections and IHC In-Reply-To: References: <200504121611.j3CGB1OD014811@chip.viawest.net> Message-ID: <6.0.0.22.1.20050415110355.01b34a80@gemini.msu.montana.edu> Anita, The matrix is the polymer which has been polymerized by UV light. It is more of a problem after IHC and using an aqueous mounting media. Several things: If your chromogen is DAB, just spend more time in alcohols and xylenes to dissolve away the polymer as this does not damage the DAB. Xylene substitutes don't work well, so keep a supply of xylene around for this purpose. For AEC where you must use an aqueous mounting media, use Crystal Mount (from Fisher), and be very generous when using it. Spread it carefully over the section and surrounding area so it really covers the section well before drying. Dry according to product insert directions and then mount a permanent coverslip over the Crystal Mount. The polymer is there but greatly diminished, less visible, or may seem to disappear all together. It fills IN the "bubbly" looking polymer matrix. Also, if you use 4X slides, the polymer left over is much worse since this is 4 times thicker than 1X slides. For bone work we found the 1/2 X slides are just as good, but we do flash the UV twice. We occasionally use 1X slides, and never 4X anymore. It would have to be the totally impossible sample before we use 4X. To do our IHC, I actually use a teflon coated razor blade to scrape away the polymer from around the section, then use a Vectro ImmEdge pen for the hydrophobic barrier. The reagents stay in place so much better without all that polymer there. At 10:37 AM 4/15/2005, you wrote: >I had problems with a 'matrix' looking background and Bernice said they >were working on a special mounting media. I know she checks the histonet >so hopefully she will answer >here. > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From rschoon <@t> email.unc.edu Fri Apr 15 12:54:36 2005 From: rschoon <@t> email.unc.edu (rschoon) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Temp/Humidity Records? In-Reply-To: References: Message-ID: <425FFFDC.1050703@email.unc.edu> I thinf that someone is either eating or drinking the wrong cactus by product. Marshall Terry Dr, Consultant Histopathologist wrote: >This differs from being a waste of time and effort in what way? > >Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > >-----Original Message----- >From: Charles, Roger [mailto:rcharles@state.pa.us] >Sent: 15 April 2005 16:36 >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Temp/Humidity Records? > > >We went thru this same ordeal a few years ago during our AAVLD >inspection. >I also had the same comments regarding the closed conditions of >automated IHC and how room temp and humidity would have little or any >affect on IHC. The inspector explained the requirement is justified >when troubleshooting any testing that has somehow been transformed over >time based on your control changes. The data would be used to rule out >the room conditions more then to blame the room conditions as the cause. >Our whole laboratory is now wired to monitor these conditions. > >Roger Charles >TSE and IHC Technician >Pa Veterinary Laboratory > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela >A. Marcum >Sent: Friday, April 15, 2005 9:46 AM >To: Marshall Terry Dr,Consultant Histopathologist >Cc: histonet@lists.utsouthwestern.edu; Breeden,Sara >Subject: RE: [Histonet] Temp/Humidity Records? > >The comment for the records were regarding IHC and yet I find changes in >humidity and temperature have more effect on my sectioning and mounting >of >paraffin and plastic sections. If you are doing automated IHC I don't >see how >this would matter. If it is manual and you use a humidity chamber it >still >won't matter much. I could only see this mattering to IHC if it is done >on the >bench in open air. The changes I get seasons change throughout the day >as heat >or AC are added or changed. > >Is this really a new requirement?? > > >Pam Marcum > > > > >Quoting "Marshall Terry Dr, Consultant Histopathologist" >: > > > >>So you keep accurate records. >>What can you do with this information that is actually useful? >> >>Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path >> Consultant Pathologist >> Rotherham General Hospital >> South Yorkshire >> England >> terry.marshall@rothgen.nhs.uk >> >>-----Original Message----- >>From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] >>Sent: 15 April 2005 14:30 >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] Temp/Humidity Records? >> >> >>During a recent inspection, the SOP and documentation for room >>temperature/humidity in the histo lab were noted as being absent. >>Although I document instrument temperatures, I have not kept records >> >> >on > > >>room temp/humidity. It was noted that these factors may affect IHC >>staining quality. If I am to begin documenting these parameters, I'd >>like to know if there are any guidelines in a general sense (or a >>specific one) relating to the variances and requirements. Any help >>would be appreciated! >> >>Sara A. Breeden, HT(ASCP) >>New Mexico Department of Agriculture >>Veterinary Diagnostic Services >>POBox 4700 >>Albuquerque, NM 87196 >>(505)841-2576 >>(505)841-2518 FAX >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From CCollins <@t> propathlab.com Fri Apr 15 13:35:07 2005 From: CCollins <@t> propathlab.com (Cory Collins) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Tape transfer sections and IHC Message-ID: Anita, We use a different type of tissue transfer using a substance called quick mount. It's a great help because we can cut the tissue from one slide and transfer it to numerous other slides for IHC and we get great results. My pathologist, Dr. Rodney T. Miller, has written a protocol for this technique at http://www.ihcworld.com/_books/Technical-IHC.pdf . If you have any questions you can e-mail me at ccollins@propathlab.com. Cory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Anita Jennings Sent: Friday, April 15, 2005 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tape transfer sections and IHC I had problems with a 'matrix' looking background and Bernice said they were working on a special mounting media. I know she checks the histonet so hopefully she will answer here. I am doing hrp IHC on tape transfer sections and I am experiencing a problem with unwanted staining on the coating on the polymer coated slides. Does anyone else out there do IHC on the tape sections experience this problem? Please suggest a solution to this. Thank you, Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From dlcowie <@t> prodigy.net Fri Apr 15 13:39:41 2005 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] RHS-1 microwave tissue processor for sale Message-ID: <20050415183941.17957.qmail@web81001.mail.yahoo.com> hello histonetters, My facility has a Hacker RHS-1 microwave tissue processor for sale. We purchased it new in 2002 and have only used it a handful of times. Our doc's prefer traditional processing. It has capacity for 110 cassettes and has all the upgrades. It is just taking up space. If anyone is interested, you may e-mail me privately or call me at 850-416-7251. Thanks, Dawn Cowie, HT Histology Supv. Pensacola Pathologists, PA From info <@t> instrumedics.com Fri Apr 15 14:37:51 2005 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Tape transfer sections and IHC References: Message-ID: <004c01c541f2$a2058910$6401a8c0@INSTRUMEDICS22> Anita, Your memory is excellent and in fact we did develop a mounting medium. CureMount is a mounting medium of low viscosity, which makes the background virtually invisible and the stained structures stand out beautifully. The refractive index (1.55) of the cured mounting medium matches the refractive index of the section. CureMount I (for frozen sections) and CureMount II (for paraffin sections) is dispensed onto the slide and the slide is coverslipped. The slide is placed under the CureMount Curing Lamp for 20 seconds. The slide is "dry" and can be archived the same day. For more information please visit our web site www.instrumedics.com If you have questions please call. 800-237-2772 Bernice ----- Original Message ----- From: "Anita Jennings" To: Sent: Friday, April 15, 2005 12:37 PM Subject: Re: [Histonet] Tape transfer sections and IHC > > > > > I had problems with a 'matrix' looking background and Bernice said they > were working on a special mounting media. I know she checks the histonet > so hopefully she will answer here. > > > > > > > > > > > > I am doing hrp IHC on tape transfer sections and I am experiencing a > problem > with unwanted staining on the coating on the polymer coated slides. Does > anyone else out there do IHC on the tape sections experience this problem? > Please suggest a solution to this. > Thank you, > Patsy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From CCollins <@t> propathlab.com Fri Apr 15 14:38:57 2005 From: CCollins <@t> propathlab.com (Cory Collins) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Tape transfer sections and IHC Message-ID: Sorry for not being more specific, you don't have to read the entire paper, scroll down to page 34 and take a look under the header of "Tissue Transfer Technique". Cory -----Original Message----- From: John Baker [mailto:bakerj@umich.edu] Sent: Friday, April 15, 2005 2:18 PM To: Cory Collins Subject: Re: [Histonet] Tape transfer sections and IHC Connie, fyi- ? I did not print out the 20 page paper but you may want to look at it. Have a fun weekend! John On Apr 15, 2005, at 2:35 PM, Cory Collins wrote: > Anita, > > We use a different type of tissue transfer using a substance called > quick > mount. It's a great help because we can cut the tissue from one > slide and > transfer it to numerous other slides for IHC and we get great results. > My > pathologist, Dr. Rodney T. Miller, has written a protocol for this > technique > at http://www.ihcworld.com/_books/Technical-IHC.pdf . If you have any > questions you can e-mail me at ccollins@propathlab.com. > > Cory > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Anita > Jennings > Sent: Friday, April 15, 2005 11:38 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Tape transfer sections and IHC > > > > > > > I had problems with a 'matrix' looking background and Bernice said > they > were working on a special mounting media. I know she checks the > histonet > so hopefully she will answer here. > > > > > > > > > > > > I am doing hrp IHC on tape transfer sections and I am experiencing a > problem > with unwanted staining on the coating on the polymer coated slides. > Does > anyone else out there do IHC on the tape sections experience this > problem? > Please suggest a solution to this. > Thank you, > Patsy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________________________________ > _______ > > > This e-mail may contain confidential or privileged information. If you > think you have received this e-mail > in error, please advise the sender by reply e-mail and then delete > this e-mail immediately. > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > John A. Baker Histology Unit Orthopaedic Research Laboratories The University of Michigan 400 North Ingalls, G-161 Ann Arbor, MI 48103-0486 office phone:734-936-1635 ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From tdobersztyn <@t> chmca.org Fri Apr 15 14:57:55 2005 From: tdobersztyn <@t> chmca.org (tdobersztyn@chmca.org) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] (no subject) Message-ID: Hello everyone. I wanted to let fellow histonetters know that there is a Senior Tech HT/EM position available at Akron Children's Hospital beginning Wednesday of next week. anyone interested can contact myself and I can send you more info. Electron Microscopy experience is a plus, but we are willing to train. Thanks! Theresa R Dobersztyn HT ASCP Electron Microscopy Laboratory Department of Pathology Akron Children's Hospital 1 Perkins Square Akron, Ohio 44308 330-543-8279 From CCollins <@t> propathlab.com Fri Apr 15 15:11:33 2005 From: CCollins <@t> propathlab.com (Cory Collins) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Tape transfer sections and IHC Message-ID: Dianne, you can get it from Newcomer Supply at 1-800-383-7799, catalog number 6270A. A tube of 30cc cost somewhere around $12.00. Cory -----Original Message----- From: Dianne Holmes [mailto:dholmes@anatomy.umsmed.edu] Sent: Friday, April 15, 2005 3:06 PM To: Cory Collins Subject: RE: [Histonet] Tape transfer sections and IHC cory - who makes the 'quick mount'? It was recommended to me last week and I didnt get the specifics cause I thought I could just use Permount. >>> "Cory Collins" 04/15/05 02:38PM >>> Sorry for not being more specific, you don't have to read the entire paper, scroll down to page 34 and take a look under the header of "Tissue Transfer Technique". Cory -----Original Message----- From: John Baker [mailto:bakerj@umich.edu] Sent: Friday, April 15, 2005 2:18 PM To: Cory Collins Subject: Re: [Histonet] Tape transfer sections and IHC Connie, fyi- ? I did not print out the 20 page paper but you may want to look at it. Have a fun weekend! John On Apr 15, 2005, at 2:35 PM, Cory Collins wrote: > Anita, > > We use a different type of tissue transfer using a substance called > quick > mount. It's a great help because we can cut the tissue from one > slide and > transfer it to numerous other slides for IHC and we get great results. > My > pathologist, Dr. Rodney T. Miller, has written a protocol for this > technique > at http://www.ihcworld.com/_books/Technical-IHC.pdf . If you have any > questions you can e-mail me at ccollins@propathlab.com. > > Cory > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Anita > Jennings > Sent: Friday, April 15, 2005 11:38 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Tape transfer sections and IHC > > > > > > > I had problems with a 'matrix' looking background and Bernice said > they > were working on a special mounting media. I know she checks the > histonet > so hopefully she will answer here. > > > > > > > > > > > > I am doing hrp IHC on tape transfer sections and I am experiencing a > problem > with unwanted staining on the coating on the polymer coated slides. > Does > anyone else out there do IHC on the tape sections experience this > problem? > Please suggest a solution to this. > Thank you, > Patsy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________________________________ > _______ > > > This e-mail may contain confidential or privileged information. If you > think you have received this e-mail > in error, please advise the sender by reply e-mail and then delete > this e-mail immediately. > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > John A. Baker Histology Unit Orthopaedic Research Laboratories The University of Michigan 400 North Ingalls, G-161 Ann Arbor, MI 48103-0486 office phone:734-936-1635 _____________________________________________________________________________ _ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From gcallis <@t> montana.edu Fri Apr 15 15:14:57 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] How does it work for bone In-Reply-To: References: Message-ID: <6.0.0.22.1.20050415141354.01b78b40@gemini.msu.montana.edu> Out of curiosity, how does it work for cutting undecalcified bone frozen sections and keep them on a slide? At 12:35 PM 4/15/2005, you wrote: >Anita, > >We use a different type of tissue transfer using a substance called quick >mount. It's a great help because we can cut the tissue from one slide and >transfer it to numerous other slides for IHC and we get great results. My >pathologist, Dr. Rodney T. Miller, has written a protocol for this technique >at http://www.ihcworld.com/_books/Technical-IHC.pdf . If you have any >questions you can e-mail me at ccollins@propathlab.com. > >Cory Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Fri Apr 15 15:15:16 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:58 2005 Subject: specifics needed on IHC world reference for tape transfer RE: [Histonet] Tape transfer sections and IHC In-Reply-To: References: Message-ID: <6.0.0.22.1.20050415141141.01ba2298@gemini.msu.montana.edu> You gave a great reference, but it is 53 pages long so could you specify what page to go to for this technic. Guess I am in a hurry today. At 12:35 PM 4/15/2005, you wrote: > My pathologist, Dr. Rodney T. Miller, has written a protocol for this > technique >at http://www.ihcworld.com/_books/Technical-IHC.pdf . If you have any >questions you can e-mail me at ccollins@propathlab.com. > >Cory Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From conniegrubaugh <@t> hotmail.com Fri Apr 15 15:16:30 2005 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] Microwave Processors Message-ID: Would all techs that have the time please email me about your experiences with the different microwave processors pro and con.. Connie Grubaugh From twheelock <@t> mclean.harvard.edu Fri Apr 15 15:20:46 2005 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Fri Sep 16 15:24:58 2005 Subject: [Histonet] MICROSCOPIC DIGITAL CAMERAS Message-ID: <4260221E.4040804@mclean.harvard.edu> Hi Everyone: I am in the market for a new digital camera. I use digital imaging to take a series of microscopic pictures on each case that comes through our department. These images are then put on our image archive for the research community. I have demoed the following cameras: The Leica DC 480 (5.1 megapixel) The Olympus DP70 (12.5 megapixel) The Zeiss AxioCam HR (1.3 Megapixel) Could people give me an idea of their experience using these cameras, the pros and cons, easy of use, etc. (Leica vs Olympus vs Zeiss)? The megapixel capacity seems not as important an issue to me, as comparing the overall function of each company's line of cameras, since I feel that I can pretty much extrapolate from one resolution capability to another within each company's line-up. The software seems to be about the same within each company's group of cameras. Thanks for any opinions or advice you can give me. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA 02478 617-855-3592 Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From jtaylor <@t> meriter.com Fri Apr 15 15:39:49 2005 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] CYCLIN D1 (SP4) on bone marrow biopsies Message-ID: <328CBAE62F31C642B422970E879DFADC093D2A@pcwex01> Wondering what people are using for a procedure for this antibody to get it to work on bone marrow biopsies that have been in decal solution. Please specify antibody source, antigen retrieval, decal solution used, etc. Thanks! Jean Taylor Immuno Tech General Medical Lab Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, April 15, 2005 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 17, Issue 25 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: EDTA --> pure water (John Kiernan) 2. Incredible book on mouse anatomy (Gayle Callis) 3. Re: Her2 testing (Promila Rastogi) 4. Source for adjustable workstations? (Tim Webster) 5. RE: Source for adjustable workstations? (Kristen Broomall) 6. Re: Tween charge (Jennifer MacDonald) 7. Source for adjustable workstations? (David Haagsma) 8. "Vision Biosystems is now being distributed in the US" (saya narra) 9. RE: Source for adjustable workstations? (Larry Woody) 10. Timer Search (Mitchell Jean A.) 11. Microm IHC stainer (Carol Bobrowitz) 12. RE: Processors (Bill Sinai) 13. RE: Long and short biopsy runs with one processor (Bill Sinai) 14. Distributor (saya narra) 15. Re: Histonet Posting - Tampa Area (JMyers1@aol.com) 16. RNA in situ hybridization-staining problems (Schmitz, Nicole) 17. Re: RNA in situ hybridization-staining problems (- -) 18. RCC Antibody Help (Sharon Chase) 19. RE: RCC Antibody Help (Dawson, Glen) 20. Temp/Humidity Records? (Breeden, Sara) 21. RE: Temp/Humidity Records? (Marshall Terry Dr, Consultant Histopathologist) 22. RE: Temp/Humidity Records? (Pamela A. Marcum) 23. RE: Long and short biopsy runs with one processor (Charles Scouten) 24. CD31 on Mouse (jo-ann-e.bader) 25. RE: CD31 on Mouse (Elizabeth Chlipala) 26. RE: Temp/Humidity Records? (Charles, Roger) ---------------------------------------------------------------------- Message: 1 Date: Thu, 14 Apr 2005 12:58:24 -0400 From: John Kiernan Subject: Re: [Histonet] EDTA --> pure water To: Trisha Emry Cc: Histonet@lists.utsouthwestern.edu Message-ID: <425EA130.CBBBA9BC@uwo.ca> Content-Type: text/plain; charset=us-ascii When instructions specify "water," without qualification, it is usual to use purified water. If tap water (whatever might be dissolved in it) is OK, the instructions will usually say "tap water" explicitly. EDTA has various uses; in histological work they mostly involve removal of calcium ions. If your tap water is hard (= contains calcium ions), it will use up some of the EDTA in the solution. If you know the Ca content of the tap water you can calculate the equivalent amount of wasted EDTA. One mole of calcium ions (40 grams) combines with one mole of disodium EDTA dihydrate (372 grams). For example, if a litre of tap water contains 100mg of calcium, that will consume 100X372/40 =930mg of EDTA. This would reduce the available EDTA in a 1% solution to about 0.9%. I do not know if my guess of 100ppm Ca is anywhere near reasonable for hard water, or if a certificate of tap water analysis reports the calcium content in this way. If my guesstimate is too low, hard water may eat up more than 10% of the EDTA in a 1% solution. A simple test for minimally "clean" lab water is to mix it with an equal volume of 1% aqueous silver nitrate. Any precipitate or opalescence indicates that the water contains anions with insoluble Ag salts or organic compounds that reduce Ag+. John Kiernan Anatomy, UWO, London, Canada. _______________________________________ Trisha Emry wrote: > > Is it necessary to use distilled H20 for EDTA or can you use tap? > > Is anyone using heat with EDTA? Please send details. > > Thank you, > > Trisha Emry > U of WA, Seattle ________________________________ ------------------------------ Message: 2 Date: Thu, 14 Apr 2005 11:49:30 -0600 From: Gayle Callis Subject: [Histonet] Incredible book on mouse anatomy To: Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050414114731.01b06fd0@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed A Color Atlas of Sectional Anatomy of the Mouse by Takamasa Iwaki, Hiroshi Yamashita, Toshiyuki Hayakawa Published in Japan in 15th September 2001 185 Pages Type: hardcover It costs $175.00, but is filled with spectacular color photos, text is in English and Japanese, Go to Braintree Scientific Website Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 3 Date: Thu, 14 Apr 2005 11:08:30 -0700 From: "Promila Rastogi" Subject: Re: [Histonet] Her2 testing To: , "Cindy DuBois" Message-ID: <37DC9F93CF7F864182D0463EF93D571B034B40@ISLETON2.california.biogenex.com> Content-Type: text/plain; charset="us-ascii" FYI, BioGenex recently received FDA approval for our InSite HER2/neu kit. Promila Rastogi Reagents Product Manager >Message: 17 >Date: Thu, 14 Apr 2005 09:19:51 -0700 (PDT) >From: Cindy DuBois >Subject: [Histonet] Her2 testing >To: Histonet >Message-ID: <20050414161951.64144.qmail@web32107.mail.mud.yahoo.com> >Content-Type: text/plain; charset=us-ascii >Is it still true that only the Dako Her2 is FDA approved? >If not, what everyone's preference for this antibdody? >Cindy DuBois ------------------------------ Message: 4 Date: Thu, 14 Apr 2005 14:17:22 -0400 From: "Tim Webster" Subject: [Histonet] Source for adjustable workstations? To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi All, A recent ergonomic assessment of our Histo Lab produced a seven page litany of ergonomic ills (Yay!) As we are about to renovate in stages, I was looking for a source of adjustable tables/benches/stations for embedding and microtomy. Staff height varies from 5'2" to 6'1+3/4. Adjusting the height of the chair is inadequate for the height of the benches and equipment. I have one supplier of adjustable tables (Custom-products.com) I would love someone to offer another name. (I struggle finding stuff on the web sometimes . . .) Also, how do you folks handle ergonomics for routine histo? Have a great day Tim Tim Webster Histology Specialist Northwestern Medical Center 133 Fairfield Street St Albans, VT 05478 (802) 524-1070 twebster@nmcinc.org ------------------------------ Message: 5 Date: Thu, 14 Apr 2005 14:29:44 -0400 From: Kristen Broomall Subject: RE: [Histonet] Source for adjustable workstations? To: 'Tim Webster' , histonet@lists.utsouthwestern.edu Message-ID: <9BCAC308B27CAD4196D8AC9ADF037AAA110A79B5@wlmmsx01.nemours.org> Content-Type: text/plain; charset="iso-8859-1" "Also, how do you folks handle ergonomics for routine histo?" Ibuprofen and muscle relaxers. Sorry, I couldn't resist. (I'm not making it up though!) But seriously, I'm 5'2" & get horrible muscle spasms in my shoulder from sitting at the microtome for long periods at a time, no matter how high the chair is positioned. I must stretch my body in some funky direction to reach the wheel. I've gotten used to using the automated part of the microtome with the foot pedal. I'd love to hear what you come up with! Kristen Broomall -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tim Webster Sent: Thursday, April 14, 2005 2:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Source for adjustable workstations? Hi All, A recent ergonomic assessment of our Histo Lab produced a seven page litany of ergonomic ills (Yay!) As we are about to renovate in stages, I was looking for a source of adjustable tables/benches/stations for embedding and microtomy. Staff height varies from 5'2" to 6'1+3/4. Adjusting the height of the chair is inadequate for the height of the benches and equipment. I have one supplier of adjustable tables (Custom-products.com) I would love someone to offer another name. (I struggle finding stuff on the web sometimes . . .) Also, how do you folks handle ergonomics for routine histo? Have a great day Tim Tim Webster Histology Specialist Northwestern Medical Center 133 Fairfield Street St Albans, VT 05478 (802) 524-1070 twebster@nmcinc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 14 Apr 2005 11:34:28 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Tween charge To: Dorothy.L.Webb@HealthPartners.Com Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I think this would fall under the category of soaking, like overdehydrated blocks. There is no charge for this. Dorothy.L.Webb@HealthPartners.Com Sent by: histonet-bounces@lists.utsouthwestern.edu 04/14/2005 07:30 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Tween charge Does anyone know of the "charge" that should be used for softening toe or fingernails to prepare for processing? Could a decal charge be safely used? It seems to me that with taking the time and the solutions involved, there should be some sort of charge incurred. Any thoughts and thanks much!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 14 Apr 2005 14:43:23 -0400 From: "David Haagsma" Subject: [Histonet] Source for adjustable workstations? To: , "Tim Webster" Message-ID: <19E3602A16438E48B51A4250CA04B5F60F98CC@exchange.marketlab.com> Content-Type: text/plain; charset="US-ASCII" Tim, MarketLab Inc. has adjustable microtome stations, http://www.marketlabinc.com/products/product.cfm/ML3158 And adjustable height grossing stations http://www.marketlabinc.com/products/product.cfm/ML3217 http://www.marketlabinc.com/products/categorydetail.cfm/722 And Microtome Arm Rests http://www.marketlabinc.com/products/product.cfm/ML1038L Thank you, Dave Haagsma www.marketlabinc.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Webster Sent: Thursday, April 14, 2005 2:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Source for adjustable workstations? Hi All, A recent ergonomic assessment of our Histo Lab produced a seven page litany of ergonomic ills (Yay!) As we are about to renovate in stages, I was looking for a source of adjustable tables/benches/stations for embedding and microtomy. Staff height varies from 5'2" to 6'1+3/4. Adjusting the height of the chair is inadequate for the height of the benches and equipment. I have one supplier of adjustable tables (Custom-products.com) I would love someone to offer another name. (I struggle finding stuff on the web sometimes . . .) Also, how do you folks handle ergonomics for routine histo? Have a great day Tim Tim Webster Histology Specialist Northwestern Medical Center 133 Fairfield Street St Albans, VT 05478 (802) 524-1070 twebster@nmcinc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 14 Apr 2005 11:46:26 -0700 (PDT) From: saya narra Subject: [Histonet] "Vision Biosystems is now being distributed in the US" To: histonet@lists.utsouthwestern.edu Message-ID: <20050414184626.52381.qmail@web60620.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi Rick ! It appears your automated immunos are overpriced compared to Dako. As a general rule, it's not good to advertise what isn't competitive in a given market. Business 101. Also, you said " Vision Biosystems is now being distributed in the U.S. ". Who is the distributor ? Can you get me the Company info so I may contact them re pricing, service etc. I wasn't aware that Vision Biosystems had found a "distributor". What is the website of the Distributor ? Is it Leica ? Saya Narra UofT __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ ------------------------------ Message: 9 Date: Thu, 14 Apr 2005 11:49:58 -0700 (PDT) From: Larry Woody Subject: RE: [Histonet] Source for adjustable workstations? To: histonet@lists.utsouthwestern.edu Message-ID: <20050414184959.12724.qmail@web52606.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii I have degenerative disc disease in my neck from 25 yrs of cutting but what may surprise you is that standing up to cut bothers me a whole lot less than sitting down, and it is less pressure on the back to stand up and I use a lot less ibuprofen. Larry --------------------------------- Do you Yahoo!? Yahoo! Small Business - Try our new resources site! ------------------------------ Message: 10 Date: Thu, 14 Apr 2005 14:29:14 -0500 From: "Mitchell Jean A." Subject: [Histonet] Timer Search To: Message-ID: <583D3E9A1E843445BD54E461D1A2F6F30F83F596@uwhis-xchng2.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" I am on a search for the large (approx 3 1/2 in square) grey winding (120 minute) timers that were ever so popular in the lab once upon a time. Anyone have a clue if they are still available anywhere? I've been searching for awhile & can't come up with a source. Thanks much, Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Department of Neurology, Neuromuscular Laboratory Madison, WI ------------------------------ Message: 11 Date: Thu, 14 Apr 2005 15:30:39 -0500 From: Carol Bobrowitz Subject: [Histonet] Microm IHC stainer To: "'Histonet (E-mail)" Message-ID: <59DF6640CDC0D7119DF000D0B761393C1A965A@thor.phys.mcw.edu> Content-Type: text/plain; charset="iso-8859-1" I'm curious. Any Histology techs out there that have demo'd the Microm IHC stainer? What are your comments? If Richard Allan was purchased by Fisher, who does the repairs and is there a yearly service contract? Vendors, Please do not send me any information. I would like comments from users not a sales pitch. Thanks, Carol Ann Bobrowitz Histology Laboratory Department of Physiology - Room 541 Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, Wisconsin 53226 414-456-8179 FAX 414-456-6546 ------------------------------ Message: 12 Date: Fri, 15 Apr 2005 07:24:48 +1000 From: "Bill Sinai" Subject: [Histonet] RE: Processors To: "'saya narra'" , "histonet \(E-mail\)" Message-ID: <001401c54138$632f85c0$0ecd080a@wsahs.nsw.gov.au> Content-Type: text/plain; charset="US-ASCII" I believe they are looking for a distributor in the USA. I have heard no rumour about the company being for sale. Histology equipment is but a small part of their whole business. Mt Waverley is in Victoria (the smallest state in Australia) I am in the outer suburbs of Sydney in NSW. The distance between is 1000klm (700miles) Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: saya narra [mailto:sayanarra@yahoo.com] Sent: Thursday, 14 April 2005 7:43 PM To: histonet@lists.utsouthwestern.edu Cc: bills@icpmr.wsahs.nsw.gov.au Subject: Processors Hello Bill, You said your VIP was reliable . I agree, we have one too and it is a good instrument. It seems no one may get a chance to get a Peloris as the competition says Vision Biosystems is FOR SALE or looking for a distributor in the USA. You are in Australia and the Histology field. Thie website says they are in Mt.Waverly, VIC, Australia. Is that near you? Have you heard any word on their Boss going door to door looking for a buyer in the USA ? Saya Narra UofT __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. ------------------------------ Message: 13 Date: Fri, 15 Apr 2005 07:35:31 +1000 From: "Bill Sinai" Subject: RE: [Histonet] Long and short biopsy runs with one processor To: , "histonet \(E-mail\)" Message-ID: <001501c54139$e27d9730$0ecd080a@wsahs.nsw.gov.au> Content-Type: text/plain; charset="US-ASCII" We occasionally run cell blocks but most times they are put through the routine overnight processing. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: dahmed@mdanderson.org [mailto:dahmed@mdanderson.org] Sent: Thursday, 14 April 2005 11:13 PM To: Bill Sinai Subject: RE: [Histonet] Long and short biopsy runs with one processor What about cell blocks? Do you feel that your currenct processing schedule for biopsies would be appropriate for cytology cell blocks? Our current bx/cell block protocol is 20 minutes in all stations including formalin for a total run time of 6 hours? Can I trim this back a little? David S. Ahmed Chief Histology Technician Department of Pathology M.D. Anderson Cancer Center "Bill Sinai" To: Sent by: "'Roxanne Soto'" , "histonet (E-mail)" histonet-bounces@lists.utsouth western.edu cc: 04/13/2005 04:55 PM Subject: RE: [Histonet] Long and short biopsy runs with one processor Roxanne, Most are the small GE biopsies >3mm in diamater or "thin" slices of any urgents >3mm thick. Solution Time Formalin 0min 70%ethanol 5min 95% 5min 100% 5min 100% 10min 100% 10min Xylol 5min Xylol 5min Xylol 10min Wax 5min Wax 10min Wax 10min Temperature ambient with Pressur/Vacuum on all stations. Two Leica ASP300 and two VIP 3000 Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: Roxanne Soto [mailto:godsgirlnow@MSN.COM] Sent: Thursday, 14 April 2005 7:37 AM To: Bill Sinai Subject: Re: [Histonet] Long and short biopsy runs with one processor What size is the tissue on the 2 hour run? How long is each station? What kind of processor? Do you use the vacuum? Roxanne ----- Original Message ----- From: Bill Sinai To: histonet (E-mail) Sent: Wednesday, April 13, 2005 5:30 PM Subject: RE: [Histonet] Long and short biopsy runs with one processor Julia, I agree with your philosophy about small runs. We are a pathology department supporting a large teaching hospital and an even larger area pathology service. However, we also do a considerable amount of private work for several endoscopy clinics in our locality. The scenario you describe is very similar to ours. We have the usual overnight run, usually 450-600 blocks with the large material in one processor and the smaller in another. We receive the endoscopy specimens anytime from 7:30am through to 3:00pm each day, with any pick ups after 6:00pm being processed the next morning in a short run 2-2.5hrs. This means we can have at least two runs per day of endoscopy specimens. The endoscopy specimens from the private clinics are about 30% of our work and the TAT for these specimens is >2 days from pick-up to hard copy result to the clinician. We also process renal biopsies several times per day as well as the occasional urgent specimen. Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Julia Dahl Sent: Thursday, 14 April 2005 4:13 AM To: Joyce.Rush@sjmcmn.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Long and short biopsy runs with one processor Joyce - First let me preface with my bias - I am a GI pathologist (read small biopsy material is what I live and breathe). Long processor runs are great for standard surgical material - but absolutely overprocess small pieces of tissue, resulting in hard, dehydrated, difficult to cut little brittle fragments. The best approach that I've seen and used is to examine your "bottlenecks." The main bottlenecks are the points at which you have lots to do in front of you - with limited resource to do it (i.e. you have 150 cassettes to embed coming off of the processor at one time and ONE embedding station. That's a bottleneck.) What time do you usually start your processor? Say your processor starts at 10:00 p.m. with a standard 6 hour process run. Your pathologists or your PAs close the grossing stations at 6:00 p.m. and load the processor - and everything sits there for 4 hours (that's another bottleneck). In this scenario - you can add a second processor run at virtually any time that you are staffed that allows you to turn over the processor for the standard run by 6:00 p.m. (when the standard next load is). Since a small biopsy run usually takes about 2 hours on the Vitek: 0600 - Standard processor emptied - large surgical cases ONLY (about 2/3 of your blocks) 0600 - 1000: Embed, cut, stain and coverslip surgicals 0700 - 0800: Previous day's and early AM courier run of small biopsies grossed in 0800 - 1000: Small biopsy processor run 1000 - small biopsy processor emptied 1000 - 1230: Embed, cut, stain and coverslip biopsies. If you wanted to do another biopsy run - to allow same day turnaround time - the 2nd biopsy run could be scheduled at 10:30 each day with slides coming out around 3:30. I'm interested to hear other people's ideas. Julia >From: "Rush, Joyce" >To: >Subject: [Histonet] Long and short biopsy runs with one processor >Date: Wed, 13 Apr 2005 12:57:07 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by mc7-f23.hotmail.com >with Microsoft SMTPSVC(6.0.3790.211); Wed, 13 Apr 2005 10:58:11 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1DLm7H-0004v5-7P; Wed, 13 Apr >2005 12:57:36 -0500 >Received: from [199.165.152.166] (helo=swlx166.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1DLm6v-0004uy-Kqfor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 12:57:19 -0500 >Received: from [66.173.44.122] (helo=keys.sjmcmn.org)by swlx166.swmed.edu >with esmtp (Exim 4.44) id 1DLm6u-0004b0-5Ffor >Histonet@lists.utsouthwestern.edu; Wed, 13 Apr 2005 12:57:09 -0500 >Received: from keys.sjmcmn.org (unknown [127.0.0.1])by keys.sjmcmn.org >(Postfix) with ESMTP id 50777256FE9for >;Wed, 13 Apr 2005 12:57:07 -0500 (CDT) >Received: from mail.SJMCMN.ORG ([10.1.0.6])by keys.sjmcmn.org (PGP >Universal service);Wed, 13 Apr 2005 12:57:07 -0500 >X-Message-Info: cmh8WMAfDXqeqGy5Qdhsq/iJQn/CwXc1jm673ZQpEo0= >X-MimeOLE: Produced By Microsoft Exchange V6.5.6944.0 >Content-class: urn:content-classes:message >X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: Long and short biopsy >runs with one processor >Thread-Index: AcVAUjUG93SXzh8HQoGO/FSJK5JRgA== >X-Scan-Signature: 5ce762e8aa474ea7aaa37532c083de4e >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >,< mailto:histone t-request@lists.utsouthwestern.edu?subject=subscribe> >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 201832d891fa504721b2e650a5a52212 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 13 Apr 2005 17:58:11.0794 (UTC) >FILETIME=[5B956320:01C54052] > >We are a community hospital and last year processed 28,000 blocks. We are >being challenged by our Laboratory Director to run a long and short, biopsy >run daily, M-F. Currently we run everything together. We have one >processor, a VIP5. I'd love to hear of ways people have handled this. >Thanks so much! I always get such good advice from this group! > >Joyce > >Joyce A. Rush, BS, MT(ASCP) >Laboratory Manager >St Joseph's Medical Center >523 North Third Street >Brainerd, MN 56401 >Office:218-828-7500 Fax: 218-828-7510 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. ------------------------------ Message: 14 Date: Thu, 14 Apr 2005 18:20:11 -0700 (PDT) From: saya narra Subject: [Histonet] Distributor To: histonet@lists.utsouthwestern.edu Message-ID: <20050415012011.27372.qmail@web60617.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Rick Couture, Thank you for the information. I will speak with our Lab Manager and have her contact the Distributor of the Vision Biosystem Instruments directly, should we decide on the need to purchase. Who was the Distributor you spoke of again ? saya narra UofT __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ ------------------------------ Message: 15 Date: Fri, 15 Apr 2005 00:48:07 EDT From: JMyers1@aol.com Subject: [Histonet] Re: Histonet Posting - Tampa Area To: histonet@lists.utsouthwestern.edu Message-ID: <1ab.36836df6.2f90a187@aol.com> Content-Type: text/plain; charset="US-ASCII" Carol: I am quite familiar with this institution; I worked as the lab manager in another hospital that's just a few miles away, and I've known several of the techs who have worked here. Please feel free to call/write me... Joe Myers, M.S., CT(ASCP) Clearwater, FL 727-504-0250 ******************************** Message: 8 Date: Wed, 13 Apr 2005 14:09:01 -0500 From: cjohnston@mdanderson.org Subject: [Histonet] Tampa Area To: histonet@pathology.swmed.edu I am looking for anyone familiar with Sun Coast Hospital in the Tampa area. If you could email me privately, I would appreciate it . Carol M. Johnston HT(ASCP) Division of Surgery M.D. Anderson Cancer Center 1515 Holcombe Blvd. Unit 017 Houston, Texas 77030 713-745-4625 fax: 713-745-2548 ------------------------------ Message: 16 Date: Fri, 15 Apr 2005 09:54:26 +0200 From: "Schmitz, Nicole" Subject: [Histonet] RNA in situ hybridization-staining problems To: Message-ID: <320709412D172C48A73DA1D2D0BE20820184AFD1@UKMSRV032.ukmuenster.de> Content-Type: text/plain; charset="iso-8859-1" In our lab we perform RNA in situ hybridizations on paraffin sections. We use the Roche DIG Labelling Kit and the Nucleic Detection Kit (AP-conjugated antibody and NBT/BCIP staining). We tried to optimize the protocol but there are still some problems occuring. After getting rid of the background staining, which took quite a while we now have the problem that after the stopping of the final staining reaction the sections go on turning darker and darker over the next few days. Even the sense probe turns dark and if there is a high mRNA expression the sections sometimes turn so dark that you can see nothing anymore except the dark colour. Has anyone encountered that kind of problem before and has any suggestions? The problem is that we have to store the slides so just taking pictures right after staining is not the best solution.. And I have another question. While trying to solve the problem I found out that some people use an Poly d(T) oligonucleotide to measure RNA degradation in the tissue. Does anyone know more about that e.g. where to order and how to use? Would I have to use another protocol for that than my normal in situ protocol? Thank you for your help Sincerely, Nicole Schmitz ------------------------------ Message: 17 Date: Fri, 15 Apr 2005 03:42:50 -0700 (PDT) From: - - Subject: Re: [Histonet] RNA in situ hybridization-staining problems To: histonet@lists.utsouthwestern.edu Message-ID: <20050415104250.78402.qmail@web42302.mail.yahoo.com> Content-Type: text/plain; charset=us-ascii Nicole, I encountered the same problem and switched to the HRP-conjugated anti-DIG antibody also by Roche. I used a TSA amplification system (Biogenex - mainly used for their machines, but I was able to use it by hand also) and finally, detected with DAB. All my samples come out great! There was need for some adjustment as I was getting background initially. But I got rid of that with time adjustment and blocking steps. I hope this helps. "Schmitz, Nicole" wrote: In our lab we perform RNA in situ hybridizations on paraffin sections. We use the Roche DIG Labelling Kit and the Nucleic Detection Kit (AP-conjugated antibody and NBT/BCIP staining). We tried to optimize the protocol but there are still some problems occuring. After getting rid of the background staining, which took quite a while we now have the problem that after the stopping of the final staining reaction the sections go on turning darker and darker over the next few days. Even the sense probe turns dark and if there is a high mRNA expression the sections sometimes turn so dark that you can see nothing anymore except the dark colour. Has anyone encountered that kind of problem before and has any suggestions? The problem is that we have to store the slides so just taking pictures right after staining is not the best solution.. And I have another question. While trying to solve the problem I found out that some people use an Poly d(T) oligonucleotide to measure RNA degradation in the tissue. Does anyone know more about that e.g. where to order and how to use? Would I have to use another protocol for that than my normal in situ protocol? Thank you for your help Sincerely, Nicole Schmitz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. ------------------------------ Message: 18 Date: Fri, 15 Apr 2005 09:01:07 -0300 From: "Sharon Chase" Subject: [Histonet] RCC Antibody Help To: Message-ID: Content-Type: text/plain; charset=US-ASCII I am trying to establish a protocol for Vector's renal cell carcinoma antibody. The data sheet states trypsin digestion for 30 minutes. I obtain negative results with trypsin digestion using normal kidney and clear cell carcinoma as controls. I then did HIER retrieval using Biocare's reveal and borg solutions in their decloaker. The normal kidney's proximal tubules stained but the clear cell was patchy. Repeating the run on the following day, using the same reagents and working dilution, I obtained weak results with normal kidney and negative tumor cells. A fresh daily working dilution(1/50), I can obtain constant normal kidney staining but usually negative tumor cells. I tried another tumor control block and leaving the antibody overnight. Normal kidney is fine. Open to any suggestions. Sharon Chase Atlantic Health Science Center Saint John NB Canada ------------------------------ Message: 19 Date: Fri, 15 Apr 2005 07:28:27 -0500 From: "Dawson, Glen" Subject: RE: [Histonet] RCC Antibody Help To: Sharon Chase , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Sharon, I've recently tried the RCC from Biocare and it works very well. It comes as a pre-dilute so just follow the accompanying instructions and the work-up is extremely easy. My only detraction is that it is an abnormally expen$ive antibody. Good Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: Sharon Chase [mailto:chash@reg2.health.nb.ca] Sent: Friday, April 15, 2005 6:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RCC Antibody Help I am trying to establish a protocol for Vector's renal cell carcinoma antibody. The data sheet states trypsin digestion for 30 minutes. I obtain negative results with trypsin digestion using normal kidney and clear cell carcinoma as controls. I then did HIER retrieval using Biocare's reveal and borg solutions in their decloaker. The normal kidney's proximal tubules stained but the clear cell was patchy. Repeating the run on the following day, using the same reagents and working dilution, I obtained weak results with normal kidney and negative tumor cells. A fresh daily working dilution(1/50), I can obtain constant normal kidney staining but usually negative tumor cells. I tried another tumor control block and leaving the antibody overnight. Normal kidney is fine. Open to any suggestions. Sharon Chase Atlantic Health Science Center Saint John NB Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Fri, 15 Apr 2005 07:30:15 -0600 From: "Breeden, Sara" Subject: [Histonet] Temp/Humidity Records? To: Message-ID: Content-Type: text/plain; charset="us-ascii" During a recent inspection, the SOP and documentation for room temperature/humidity in the histo lab were noted as being absent. Although I document instrument temperatures, I have not kept records on room temp/humidity. It was noted that these factors may affect IHC staining quality. If I am to begin documenting these parameters, I'd like to know if there are any guidelines in a general sense (or a specific one) relating to the variances and requirements. Any help would be appreciated! Sara A. Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX ------------------------------ Message: 21 Date: Fri, 15 Apr 2005 14:38:50 +0100 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] Temp/Humidity Records? To: "Breeden, Sara" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" So you keep accurate records. What can you do with this information that is actually useful? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] Sent: 15 April 2005 14:30 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Temp/Humidity Records? During a recent inspection, the SOP and documentation for room temperature/humidity in the histo lab were noted as being absent. Although I document instrument temperatures, I have not kept records on room temp/humidity. It was noted that these factors may affect IHC staining quality. If I am to begin documenting these parameters, I'd like to know if there are any guidelines in a general sense (or a specific one) relating to the variances and requirements. Any help would be appreciated! Sara A. Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Fri, 15 Apr 2005 09:46:10 -0400 From: "Pamela A. Marcum" Subject: RE: [Histonet] Temp/Humidity Records? To: "Marshall Terry Dr, Consultant Histopathologist" Cc: histonet@lists.utsouthwestern.edu, "Breeden, Sara" Message-ID: <1113572770.425fc5a21ff35@imp.vet.upenn.edu> Content-Type: text/plain; charset=ISO-8859-1 The comment for the records were regarding IHC and yet I find changes in humidity and temperature have more effect on my sectioning and mounting of paraffin and plastic sections. If you are doing automated IHC I don't see how this would matter. If it is manual and you use a humidity chamber it still won't matter much. I could only see this mattering to IHC if it is done on the bench in open air. The changes I get seasons change throughout the day as heat or AC are added or changed. Is this really a new requirement?? Pam Marcum Quoting "Marshall Terry Dr, Consultant Histopathologist" : > So you keep accurate records. > What can you do with this information that is actually useful? > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] > Sent: 15 April 2005 14:30 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temp/Humidity Records? > > > During a recent inspection, the SOP and documentation for room > temperature/humidity in the histo lab were noted as being absent. > Although I document instrument temperatures, I have not kept records on > room temp/humidity. It was noted that these factors may affect IHC > staining quality. If I am to begin documenting these parameters, I'd > like to know if there are any guidelines in a general sense (or a > specific one) relating to the variances and requirements. Any help > would be appreciated! > > Sara A. Breeden, HT(ASCP) > New Mexico Department of Agriculture > Veterinary Diagnostic Services > POBox 4700 > Albuquerque, NM 87196 > (505)841-2576 > (505)841-2518 FAX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 23 Date: Fri, 15 Apr 2005 09:45:13 -0500 From: "Charles Scouten" Subject: RE: [Histonet] Long and short biopsy runs with one processor To: "Rush, Joyce" , Message-ID: <5784D843593D874C93E9BADCB87342AB44F84E@tpiserver03.Coretech-holdings.com> Content-Type: text/plain; charset="iso-8859-1" We offer a processor able to simultaneously process two runs, so you can be doing the long run while ripping through several short runs. It is a dip and dunk system, like a stainer, but has thorough vapor emission control. This style has less carryover and mixing than a single tank systems, and lets more than one run be processing at any one time. The TPC 15 DUO Tissue Processor Product: #511101 from Vibratome, made by Medite TPC 15 DUO Tissue Processor http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=511101&catdesc=Histology+Equipment&CatThreeID=595&CatOneID=4&subcatdesc=Tissue+Processing+Systems&idsubcategory=180 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rush, Joyce Sent: Wednesday, April 13, 2005 12:57 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Long and short biopsy runs with one processor We are a community hospital and last year processed 28,000 blocks. We are being challenged by our Laboratory Director to run a long and short, biopsy run daily, M-F. Currently we run everything together. We have one processor, a VIP5. I'd love to hear of ways people have handled this. Thanks so much! I always get such good advice from this group! Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 24 Date: Fri, 15 Apr 2005 10:50:55 -0400 From: "jo-ann-e.bader" Subject: [Histonet] CD31 on Mouse To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Good morning everyone, I have a couple of labs in our group who would like to do immuno with CD31 on formalin fixed, paraffin, mouse tissue. ?they cannot seem to find a method that works. Any advise will be appreciated. Thank you in advance Jo-Ann Bader -- Director's Assistant Molecular Oncology Group McGill University Health Centre 687 Pine Ave. W. Room H5-21 Montreal, QC,Canada, H3A-1A1 Tel: 514-843-1479 Fax; 514-843-1478 E-mail: jo-ann.bader@mcgill.ca ------------------------------ Message: 25 Date: Fri, 15 Apr 2005 09:17:32 -0600 From: "Elizabeth Chlipala" Subject: RE: [Histonet] CD31 on Mouse To: "'jo-ann-e.bader'" , "'Histonet'" Message-ID: <000201c541ce$424713f0$76d48a80@AMY> Content-Type: text/plain; charset="US-ASCII" Jo-Ann We use the goat polyclonal from santa cruz. Works great on FFPE tissue. I'll send you the protocol and image off the histonet. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jo-ann-e.bader Sent: Friday, April 15, 2005 7:51 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CD31 on Mouse Good morning everyone, I have a couple of labs in our group who would like to do immuno with CD31 on formalin fixed, paraffin, mouse tissue. ?they cannot seem to find a method that works. Any advise will be appreciated. Thank you in advance Jo-Ann Bader -- Director's Assistant Molecular Oncology Group McGill University Health Centre 687 Pine Ave. W. Room H5-21 Montreal, QC,Canada, H3A-1A1 Tel: 514-843-1479 Fax; 514-843-1478 E-mail: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 26 Date: Fri, 15 Apr 2005 11:36:29 -0400 From: "Charles, Roger" Subject: RE: [Histonet] Temp/Humidity Records? To: Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57006C557D@enhbgpri04.pa.lcl> Content-Type: text/plain; charset="us-ascii" We went thru this same ordeal a few years ago during our AAVLD inspection. I also had the same comments regarding the closed conditions of automated IHC and how room temp and humidity would have little or any affect on IHC. The inspector explained the requirement is justified when troubleshooting any testing that has somehow been transformed over time based on your control changes. The data would be used to rule out the room conditions more then to blame the room conditions as the cause. Our whole laboratory is now wired to monitor these conditions. Roger Charles TSE and IHC Technician Pa Veterinary Laboratory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela A. Marcum Sent: Friday, April 15, 2005 9:46 AM To: Marshall Terry Dr,Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu; Breeden,Sara Subject: RE: [Histonet] Temp/Humidity Records? The comment for the records were regarding IHC and yet I find changes in humidity and temperature have more effect on my sectioning and mounting of paraffin and plastic sections. If you are doing automated IHC I don't see how this would matter. If it is manual and you use a humidity chamber it still won't matter much. I could only see this mattering to IHC if it is done on the bench in open air. The changes I get seasons change throughout the day as heat or AC are added or changed. Is this really a new requirement?? Pam Marcum Quoting "Marshall Terry Dr, Consultant Histopathologist" : > So you keep accurate records. > What can you do with this information that is actually useful? > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] > Sent: 15 April 2005 14:30 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temp/Humidity Records? > > > During a recent inspection, the SOP and documentation for room > temperature/humidity in the histo lab were noted as being absent. > Although I document instrument temperatures, I have not kept records on > room temp/humidity. It was noted that these factors may affect IHC > staining quality. If I am to begin documenting these parameters, I'd > like to know if there are any guidelines in a general sense (or a > specific one) relating to the variances and requirements. Any help > would be appreciated! > > Sara A. Breeden, HT(ASCP) > New Mexico Department of Agriculture > Veterinary Diagnostic Services > POBox 4700 > Albuquerque, NM 87196 > (505)841-2576 > (505)841-2518 FAX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 17, Issue 25 **************************************** From tdobersztyn <@t> chmca.org Fri Apr 15 15:41:48 2005 From: tdobersztyn <@t> chmca.org (tdobersztyn@chmca.org) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Job opportunity in Ohio - HT senior Tech Message-ID: Hello everyone. I wanted to let fellow histonetters know that there is a Senior Tech HT/EM position available at Akron Children's Hospital beginning Wednesday of next week. anyone interested can contact myself and I can send you more info. Electron Microscopy experience is a plus, but we are willing to train. Thanks! Theresa R Dobersztyn HT ASCP Electron Microscopy Laboratory Department of Pathology Akron Children's Hospital 1 Perkins Square Akron, Ohio 44308 330-543-8279 From POWELL_SA <@t> Mercer.edu Fri Apr 15 15:49:03 2005 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] MICROSCOPIC DIGITAL CAMERAS In-Reply-To: <4260221E.4040804@mclean.harvard.edu> Message-ID: <01LN4OUZXBYY8WZ91X@Macon2.Mercer.edu> Hi Tim, I use a Sony Cybershot DSC-717 5.0 mgpx on my Olympus BX40 using an adapter developed by Martin Microscopes www.martinmicroscope.com. Check out their web site where they list the scopes for which they have adapters. Bobby Martin is a very knowledgeable person. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Friday, April 15, 2005 3:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MICROSCOPIC DIGITAL CAMERAS Hi Everyone: I am in the market for a new digital camera. I use digital imaging to take a series of microscopic pictures on each case that comes through our department. These images are then put on our image archive for the research community. I have demoed the following cameras: The Leica DC 480 (5.1 megapixel) The Olympus DP70 (12.5 megapixel) The Zeiss AxioCam HR (1.3 Megapixel) Could people give me an idea of their experience using these cameras, the pros and cons, easy of use, etc. (Leica vs Olympus vs Zeiss)? The megapixel capacity seems not as important an issue to me, as comparing the overall function of each company's line of cameras, since I feel that I can pretty much extrapolate from one resolution capability to another within each company's line-up. The software seems to be about the same within each company's group of cameras. Thanks for any opinions or advice you can give me. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA 02478 617-855-3592 Any information, including protected health information (PHI), transmitted in this email is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential and or exempt from disclosure under applicable Federal or State law. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon, protected health information (PHI) by persons or entities other than the intended recipient is prohibited. If you received this email in error, please contact the sender and delete the material from any computer. From BDUE <@t> PARTNERS.ORG Fri Apr 15 16:03:37 2005 From: BDUE <@t> PARTNERS.ORG (Due, Brice) Date: Fri Sep 16 15:24:59 2005 Subject: Page 35(34) RE: specifics needed on IHC world reference for tape transfer RE:[Histonet] Tape transfer sections and IHC Message-ID: <59C772E2D8EDF345AE1601F5B60B3CB50279AA@PHSXMB7.partners.org> Page 34 by the document, but page 35 by Acrobat. Just do a search for "mount" once you have the pdf open. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: Friday, April 15, 2005 4:15 PM To: Cory Collins; Histonet@lists.utsouthwestern.edu Subject: specifics needed on IHC world reference for tape transfer RE:[Histonet] Tape transfer sections and IHC You gave a great reference, but it is 53 pages long so could you specify what page to go to for this technic. Guess I am in a hurry today. At 12:35 PM 4/15/2005, you wrote: > My pathologist, Dr. Rodney T. Miller, has written a protocol for this > technique >at http://www.ihcworld.com/_books/Technical-IHC.pdf . If you have any >questions you can e-mail me at ccollins@propathlab.com. > >Cory Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Apr 15 17:07:29 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] tape and other transfer methods RE: How does it work for bone In-Reply-To: References: Message-ID: <6.0.0.22.1.20050415160138.01b85a68@gemini.msu.montana.edu> Yes, these are different types of transfer. The Instrumedics tape transfer is the immediate way to pick up a section onto tape to be transfered onto a polymer coated slide which is then exposed to UV light. This can be for a paraffin block or the very popular Cryojane for undecalcifed bone frozen sections, fix and then do IHC or whatever stain one needs. These are sections normally too difficult to cut and adhere to a plus charge slide to begin with, or in the case of bone sections, totally fall apart. You can check out the device/method on Instrumedics website, they have diagrams and method description. I can see the advantages of your method for the application you describe too after reading p. 34, thanks for that info. At 02:19 PM 4/15/2005, you wrote: >We may be talking about two totally different ideas. > >What I'm talking about it transfering previously stained H&E sections to >different slides (in case the block is missing or no more tumor is left in >it) and doing the IHC stains necessary to get a diagnosis. Like I said we've >gotten up to 30 stains from one slide. It's also useful on cytology slides >and special stains. Am I making any sense? > >Cory > >-----Original Message----- >From: Gayle Callis [mailto:gcallis@montana.edu] >Sent: Friday, April 15, 2005 3:15 PM >To: Cory Collins; Histonet@lists.utsouthwestern.edu >Subject: How does it work for bone > > >Out of curiosity, how does it work for cutting undecalcified bone frozen >sections and keep them on a slide? > >At 12:35 PM 4/15/2005, you wrote: > >Anita, > > > >We use a different type of tissue transfer using a substance called quick > >mount. It's a great help because we can cut the tissue from one slide and > >transfer it to numerous other slides for IHC and we get great results. My > >pathologist, Dr. Rodney T. Miller, has written a protocol for this technique > >at http://www.ihcworld.com/_books/Technical-IHC.pdf . If you have any > >questions you can e-mail me at ccollins@propathlab.com. > > > >Cory > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 (lab with voice mail) >406 994-4303 (FAX) > > >______________________________________________________________________________ > > >This e-mail may contain confidential or privileged information. If you >think you have received this e-mail >in error, please advise the sender by reply e-mail and then delete this >e-mail immediately. From sschu <@t> Arctur.com Fri Apr 15 17:21:12 2005 From: sschu <@t> Arctur.com (Shirley S. Chu) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] California Histotechnology Convention Message-ID: To all Histonetters, The California Society for Histotechnology is holding its annual convention/symposium on May 12-15, 2005 at the DoubleTree Hotel in San Jose, CA (SF Bay Area). Workshops will be conducted each day of the meeting and exhibitors' hall open from Thurs afternoon through Sat morning. Anyone wishing an electronic copy of the program, please respond directly to me by e-mail: sschu@arctur.com Shirley Chu Arcturus Bioscience, Inc. 650-962-3020 ext. 359 From VAZQUEZR <@t> ohsu.edu Fri Apr 15 17:25:34 2005 From: VAZQUEZR <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Microwave Processors Message-ID: I will Monday, I have to leave for the day Robyn OHSU >>> "connie grubaugh" 04/15/05 1:16 PM >>> Would all techs that have the time please email me about your experiences with the different microwave processors pro and con.. Connie Grubaugh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From onep00 <@t> hotmail.com Sat Apr 16 00:39:34 2005 From: onep00 <@t> hotmail.com (Pam O'Neill) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] double impregnation of mummy tissue Message-ID: Hello All, I am reviewing a procedure for double impregnation of mummy tissue. It is from a book published in 1980. My problem is I am not familiar with the "purpose"(example: alcohol used for dehydration) of some of these solutions/chemicals in the processing steps. If someone could explain any of them maybe I could determine if there is a substitute product available. The first is 8% Phenol in 95% alcohol. The second is amyl acetate. In the double impregnation step they use 2%celloidin in methyl benzoate. What is the benefit of a double impregnation technique? The procedure instead of processing overnite is a 48 hour process. Why would you need to extend for so long? Any information will be a start in the right direction! Thankyou. Pam O'Neill BS HT/HTL (QIHC) SVMMC Toledo, Ohio _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today - it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ From arvind <@t> nbrc.res.in Fri Apr 15 12:44:41 2005 From: arvind <@t> nbrc.res.in (Arvind Pundir) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] regarding position of immunohistotech Message-ID: Dear sir, I am interested in joining your organisation at the above mentioned post, I have been working at present in National brain Research centre, Gurgaon, Haryana,India, as a senior laboratory technician my work includes doing immuno on brain tissue. I have done P. G. Diploma in Biomedical Laboratory Technology, after graduation in Microbiology I am very much interested in joining your organisation , for that please breif me what to do next Thanking you, yours' faithfully, Arvind Singh Pundir, National Brain Research Centre, Manesar, Gurgaon, Haryana, INDIA contact no--091-9811056224 From Barry.R.Rittman <@t> uth.tmc.edu Sat Apr 16 06:38:51 2005 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] double impregnation of mummy tissue Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0F3537C@UTHEVS3.mail.uthouston.edu> This is essentially Peterfi's technic. The celloidin is only present in the final block as a thin framework of celloidin that tend permeates the tissue and holds tissue together better than paraffin alone. May not be necessary to use double embedding if you are dealing with soft tissues. Peterfi's technic was designed for preparation of hard tissue samples. An alternate fiztion procedure is Sandersons technic Am at home without my notes this weekend. Hope this helps Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pam O'Neill Sent: Sat 4/16/2005 12:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] double impregnation of mummy tissue Hello All, I am reviewing a procedure for double impregnation of mummy tissue. It is from a book published in 1980. My problem is I am not familiar with the "purpose"(example: alcohol used for dehydration) of some of these solutions/chemicals in the processing steps. If someone could explain any of them maybe I could determine if there is a substitute product available. The first is 8% Phenol in 95% alcohol. The second is amyl acetate. In the double impregnation step they use 2%celloidin in methyl benzoate. What is the benefit of a double impregnation technique? The procedure instead of processing overnite is a 48 hour process. Why would you need to extend for so long? Any information will be a start in the right direction! Thankyou. Pam O'Neill BS HT/HTL (QIHC) SVMMC Toledo, Ohio _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today - it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sun Apr 17 04:03:27 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Timer Search References: <583D3E9A1E843445BD54E461D1A2F6F30F83F596@uwhis-xchng2.hosp.wisc.edu> Message-ID: <042a01c5432c$52236300$9f27d445@domainnotset.invalid> I posted a similar question for the same timers about 2 years ago. Even with all the suggestions from the wonder people on Histonet, these gray timers were never to be found - then or since. I don't believe this type of timer is being made anymore. Most labs seem to be going with digital, battery operated timers. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 38073 ----- Original Message ----- From: "Mitchell Jean A." To: Sent: Thursday, April 14, 2005 3:29 PM Subject: [Histonet] Timer Search I am on a search for the large (approx 3 1/2 in square) grey winding (120 minute) timers that were ever so popular in the lab once upon a time. Anyone have a clue if they are still available anywhere? I've been searching for awhile & can't come up with a source. Thanks much, Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Department of Neurology, Neuromuscular Laboratory Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sun Apr 17 04:13:06 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] PA Exam - additional information Message-ID: <043201c5432d$ab65ec20$9f27d445@domainnotset.invalid> Hi - I followed up on finding additional information about taking the PA exam via the OJT route - I contacted ASCP Board of Registry (BOR) about the Dec. 31 2007 deadline - as to whether it would be similar to the "deadline" that the HT high school OJT candidates have. The answer is YES, from Deborah Lyons, Assistant Manager of ASCP BOR. "Applicants determined eligible prior to the December 31, 2007 deadline for OJT eligibility will be eligible to test for a period of five years from the date of application, and yes they will have five attempts to pass the examination." In other words - if a PA OJT candidate takes the exam sometime before the Dec. 31, 2007 deadline, but fails it - they still have 4 more tries to take it (that's a total of 5 tries - the first time and 4 more tries) over the next five years. So - if you are thinking about taking the exam, and don't know if you are prepared enough - take it BEFORE the deadline anyway. If you pass it - great. If you don't pass, then you've gotten some experience with the types of questions on the exam, and you still have 4 more tries over the next five years to study, study, study. So the Dec. 31, 2007 deadline is a "deadline" for taking the PA exam the first time. (Remember, though, you still have the pay the application fee each time.) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From lpwenk <@t> sbcglobal.net Sun Apr 17 04:21:43 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Re: ASCP - My 2 cents References: <200504041439.KAA08840@arrakis.vcu.edu> Message-ID: <044101c5432e$df2aae00$9f27d445@domainnotset.invalid> Getting in late on this, because I've been away on vacation. As to whether a histotech with a higher degree or a higher certification should be paid a higher salary - In my opinion - if they are doing the SAME job as a histotech with a lower degree and/or lower certification - then NO, they should not be paid more. The person frying fries at a burger place earns the same money as the next fryer, regardless of whether they are still in high school, a HS graduate, in college, or a college graduate. It is the job that sets the pay scale, not the degree/certification. If, however, due to the higher degree or higher certification, that person is now doing additional or more complicated responsibilities - then yes, they should be paid accordingly. And if they are not being compensated for their additional responsibilities, then they have every right to grieve this to human resources. My opinions, totally. (And no, I'm not saying histotechs = fryers. Just using it as an example.) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: "Kelly D Mcqueeney" Cc: ; Sent: Monday, April 04, 2005 10:39 AM Subject: Re: [Histonet] Re: ASCP - My 2 cents > I have listened to all of this talk about certification, and no one > has yet to comment on those of us with degrees. Should a histotech > with a degree start at a higher salary base? What about certification? > Considering we are only eligible after a year in the field, we are a > lot of times neglected as far as base salary is concerned even though > we do have a degree, but are not yet certified. How should ASCP, or > even our employers address that issue? > > Candyce Fockler > Histotechnologist > VCUHS > > > > > ------------------- > > But lets say I wanted to be a Histotechnologist (is that the term?), > > > what kind of education would I need? What is the difference in > education > > for a Pathology Assistant, Technologist, etc? Sorry for the simple > > question, I read everyone's emails about certification and I'm just > > curious. Very interesting.... > > > > Kelly McQueeney > > > > > > Robyn Vazquez wrote: > > > > >Kelly, > > > > > >I have been doing histology for going on 15 years (?) and never > certified as my own choice. I do EXCELLENT QUALITY work. I was > taught in the military as OTJ training. > > > > > >Robyn > > > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sun Apr 17 04:24:44 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Taft's Method For Nucleic Acids References: <5E6BFDF4F0AB2C4DA69CF4473FC7B948323307@wala01.seattlecca.org> Message-ID: <046201c5432f$4b565480$9f27d445@domainnotset.invalid> What are you staining for? Just curious. MGP used to be used to demonstrate plasma cells (RNA in the cytoplasm), but IHC is SOOO much better. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak MI 48073 ----- Original Message ----- From: "Farley, Sunni R" To: Sent: Sunday, April 10, 2005 4:22 PM Subject: [Histonet] Taft's Method For Nucleic Acids > I am looking for information regarding Methyl Green-Pyronin staining for > nucleic acids. I have contacted Rowley Biochemical Inc (Danvers, MA) about > their Methyl Green Pyronin stain and Differentiating Solution. They > provided me with the chemical composition of these solutions and also the > protocol > for Taft's Method for Nucleic Acids. Does anybody routinely use these > items and this method? Also, have you used them on B5-fixed tissue? > Any information and/or suggestions would be much appreciated. Thank you for > your time! > > Sunni R. Farley > Histotechnician > Pathology Department > Seattle Cancer Care Alliance > 825 Eastlake Ave East > Seattle, Washington 98109 > (206) 288-1312 > > > > This electronic message transmission contains information which may be > confidential or privileged. The information is intended to be for the > use of the individual or entity named above. If you are not the > intended recipient, be aware that any disclosure, copying, distribution > or use of the contents of this information is prohibited. If you have > received this electronic transmission in error, please leave a message > via telephone at (206) 288-6266, notify me by electronic reply, and > delete this message. Opinions and ideas in this message that do not > relate to official business are understood as neither given nor > endorsed by the Seattle Cancer Care Alliance. To view our complete > Notice of Privacy Practices, visit our web site at www.seattlecca.org. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sun Apr 17 04:28:25 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Mallory PTAH and Zenkers References: Message-ID: <047501c5432f$cee0f3a0$9f27d445@domainnotset.invalid> No, you would not have to do the iodine and sodium thiosulfate step if you fix/post-mordant in something other than mercury. If you want, fix the tissue in formalin, process/embed/section as usual, and then postmordant in Bouin at 60 degree C for 1 hour before staining. Same as doing a trichrome. Wash in running water afterwards until the yellow color is gone. Stain with PTAH. Works great. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Perry, Margaret" To: Sent: Tuesday, April 12, 2005 6:04 PM Subject: [Histonet] Mallory PTAH and Zenkers Hello, I have a question about the Mallory's PTAH. If I use the zenkers without mercuric chloride do I still need to use Gram's iodine and sodium thiosulfate? I assume not but need to be sure. Thanks for the help. Margaret Perry HT South Dakota University Veterinary Science Histopathology Brookings SD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lao_ji <@t> yahoo.com Sun Apr 17 20:48:45 2005 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] RNA in situ hybridization-staining problems In-Reply-To: <20050415104250.78402.qmail@web42302.mail.yahoo.com> Message-ID: <20050418014845.28747.qmail@web30703.mail.mud.yahoo.com> Nicole, We used BM purple for AP-conjugated anti-DIG, I don't have problem like you mention, I just feel BM pruple is very stable, For more information please visit my working Lab website for protocal: http://saturn.med.nyu.edu/research/dg/joynerlab/protocols.html Hope the visualization part can help! Jimmy --- - - wrote: > Nicole, > > I encountered the same problem and switched to the > HRP-conjugated anti-DIG antibody also by Roche. I > used a TSA amplification system (Biogenex - mainly > used for their machines, but I was able to use it by > hand also) and finally, detected with DAB. All my > samples come out great! There was need for some > adjustment as I was getting background initially. > But I got rid of that with time adjustment and > blocking steps. I hope this helps. > > > "Schmitz, Nicole" wrote: > In our lab we perform RNA in situ hybridizations on > paraffin sections. We use the Roche DIG Labelling > Kit and the Nucleic Detection Kit (AP-conjugated > antibody and NBT/BCIP staining). We tried to > optimize the protocol but there are still some > problems occuring. After getting rid of the > background staining, which took quite a while we now > have the problem that after the stopping of the > final staining reaction the sections go on turning > darker and darker over the next few days. Even the > sense probe turns dark and if there is a high mRNA > expression the sections sometimes turn so dark that > you can see nothing anymore except the dark colour. > Has anyone encountered that kind of problem before > and has any suggestions? The problem is that we have > to store the slides so just taking pictures right > after staining is not the best solution.. > And I have another question. While trying to solve > the problem I found out that some people use an Poly > d(T) oligonucleotide to measure RNA degradation in > the tissue. Does anyone know more about that e.g. > where to order and how to use? Would I have to use > another protocol for that than my normal in situ > protocol? > Thank you for your help > > Sincerely, > Nicole Schmitz > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > --------------------------------- > Do you Yahoo!? > Read only the mail you want - Yahoo! Mail > SpamGuard. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Make Yahoo! your home page http://www.yahoo.com/r/hs From ashokc <@t> ncbs.res.in Mon Apr 18 01:54:50 2005 From: ashokc <@t> ncbs.res.in (ashokc@ncbs.res.in) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] cryosections Message-ID: <1179.192.168.3.133.1113807290.squirrel@192.168.3.133> Hello Dear all i need info about cryosections of mouse testis and staining,weather one can use hemotoxylin& eosin for cryosections if so, what type of hematoxlin is recommended. suggestions are well appreciated Ashok.C Research Scholar Prof.M.M.Panicker's lab Molecular Neurobiology National Centre for Biological Sciences GKVK Campus Bangalore-560065 Phone-+91-080-23636421 ext :2251 Fax no-91-080-23636662 From akbitting <@t> geisinger.edu Mon Apr 18 07:05:39 2005 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Amyloid control Message-ID: Does anyone have a spare amyloid control block for Thio T stains? Right now, we're hard pressed to find a good one in house. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Terry.Marshall <@t> rothgen.nhs.uk Mon Apr 18 07:39:30 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Temp/Humidity Records? Message-ID: Grrrr. :-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Charles, Roger [mailto:rcharles@state.pa.us] Sent: 15 April 2005 17:54 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? This differs in being an accredited lab vs a non-accredited lab. -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Friday, April 15, 2005 11:45 AM To: Charles, Roger; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? This differs from being a waste of time and effort in what way? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Charles, Roger [mailto:rcharles@state.pa.us] Sent: 15 April 2005 16:36 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? We went thru this same ordeal a few years ago during our AAVLD inspection. I also had the same comments regarding the closed conditions of automated IHC and how room temp and humidity would have little or any affect on IHC. The inspector explained the requirement is justified when troubleshooting any testing that has somehow been transformed over time based on your control changes. The data would be used to rule out the room conditions more then to blame the room conditions as the cause. Our whole laboratory is now wired to monitor these conditions. Roger Charles TSE and IHC Technician Pa Veterinary Laboratory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela A. Marcum Sent: Friday, April 15, 2005 9:46 AM To: Marshall Terry Dr,Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu; Breeden,Sara Subject: RE: [Histonet] Temp/Humidity Records? The comment for the records were regarding IHC and yet I find changes in humidity and temperature have more effect on my sectioning and mounting of paraffin and plastic sections. If you are doing automated IHC I don't see how this would matter. If it is manual and you use a humidity chamber it still won't matter much. I could only see this mattering to IHC if it is done on the bench in open air. The changes I get seasons change throughout the day as heat or AC are added or changed. Is this really a new requirement?? Pam Marcum Quoting "Marshall Terry Dr, Consultant Histopathologist" : > So you keep accurate records. > What can you do with this information that is actually useful? > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] > Sent: 15 April 2005 14:30 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temp/Humidity Records? > > > During a recent inspection, the SOP and documentation for room > temperature/humidity in the histo lab were noted as being absent. > Although I document instrument temperatures, I have not kept records on > room temp/humidity. It was noted that these factors may affect IHC > staining quality. If I am to begin documenting these parameters, I'd > like to know if there are any guidelines in a general sense (or a > specific one) relating to the variances and requirements. Any help > would be appreciated! > > Sara A. Breeden, HT(ASCP) > New Mexico Department of Agriculture > Veterinary Diagnostic Services > POBox 4700 > Albuquerque, NM 87196 > (505)841-2576 > (505)841-2518 FAX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Mon Apr 18 08:20:22 2005 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Timer Search In-Reply-To: <583D3E9A1E843445BD54E461D1A2F6F30F83F596@uwhis-xchng2.hosp.wisc.edu> References: <583D3E9A1E843445BD54E461D1A2F6F30F83F596@uwhis-xchng2.hosp.wisc.edu> Message-ID: <4263B416.4000705@bitstream.net> Hi Jean, The "wind-up" analog timer that you are looking for was originally manufactured by General Electric - "GE". (Which I always thought strange.... that GE would make a non-electrical appliance). Any how, it has not been manufactured for many years. They do pop up from time to time through the used lab equipment companies. I actually have one that I took home and use in my kitchen - great timer! (not for sale, sorry). I may know a place that has them here locally, so contact me "off-list" for more info. ~ Ford Ford M. Royer, MT(ASCP) Midwest Science & Biocenter Minneapolis, MN 55301 800-475-4869 Mitchell Jean A. wrote: >I am on a search for the large (approx 3 1/2 in square) grey winding >(120 minute) timers that were ever so popular in the lab once upon a >time. Anyone have a clue if they are still available anywhere? > >I've been searching for awhile & can't come up with a source. > >Thanks much, >Jean Mitchell, BS, HT (ASCP) >University of Wisconsin Hospital & Clinics >Department of Neurology, Neuromuscular Laboratory >Madison, WI >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From lchung <@t> ppmh.org Mon Apr 18 08:13:34 2005 From: lchung <@t> ppmh.org (Chung, Luong) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Cryostat Message-ID: All, I'm looking for a reliable cryostat with self disinfection. Any suggestion? Thanks Bruce Chung, MSM, HT/CT(ASCP) Anatomic Pathology Manager Phoebe Putney Memorial Hospital lchung@ppmh.org From Kemlo.Rogerson <@t> elht.nhs.uk Mon Apr 18 09:20:25 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Temp/Humidity Records?[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F1B5@bhrv-nt-11.bhrv.nwest.nhs.uk> This differs from a scientific approach to a QMS to a non-scientific approach; a medical approach? Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Marshall Terry Dr,Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 18 April 2005 13:40 To: Charles, Roger; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records?[Scanned] Grrrr. :-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Charles, Roger [mailto:rcharles@state.pa.us] Sent: 15 April 2005 17:54 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? This differs in being an accredited lab vs a non-accredited lab. -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Friday, April 15, 2005 11:45 AM To: Charles, Roger; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? This differs from being a waste of time and effort in what way? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Charles, Roger [mailto:rcharles@state.pa.us] Sent: 15 April 2005 16:36 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? We went thru this same ordeal a few years ago during our AAVLD inspection. I also had the same comments regarding the closed conditions of automated IHC and how room temp and humidity would have little or any affect on IHC. The inspector explained the requirement is justified when troubleshooting any testing that has somehow been transformed over time based on your control changes. The data would be used to rule out the room conditions more then to blame the room conditions as the cause. Our whole laboratory is now wired to monitor these conditions. Roger Charles TSE and IHC Technician Pa Veterinary Laboratory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela A. Marcum Sent: Friday, April 15, 2005 9:46 AM To: Marshall Terry Dr,Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu; Breeden,Sara Subject: RE: [Histonet] Temp/Humidity Records? The comment for the records were regarding IHC and yet I find changes in humidity and temperature have more effect on my sectioning and mounting of paraffin and plastic sections. If you are doing automated IHC I don't see how this would matter. If it is manual and you use a humidity chamber it still won't matter much. I could only see this mattering to IHC if it is done on the bench in open air. The changes I get seasons change throughout the day as heat or AC are added or changed. Is this really a new requirement?? Pam Marcum Quoting "Marshall Terry Dr, Consultant Histopathologist" : > So you keep accurate records. > What can you do with this information that is actually useful? > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] > Sent: 15 April 2005 14:30 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temp/Humidity Records? > > > During a recent inspection, the SOP and documentation for room > temperature/humidity in the histo lab were noted as being absent. > Although I document instrument temperatures, I have not kept records on > room temp/humidity. It was noted that these factors may affect IHC > staining quality. If I am to begin documenting these parameters, I'd > like to know if there are any guidelines in a general sense (or a > specific one) relating to the variances and requirements. Any help > would be appreciated! > > Sara A. Breeden, HT(ASCP) > New Mexico Department of Agriculture > Veterinary Diagnostic Services > POBox 4700 > Albuquerque, NM 87196 > (505)841-2576 > (505)841-2518 FAX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Apr 18 10:33:31 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Methyl green pyronin staining, methyl green source In-Reply-To: <046201c5432f$4b565480$9f27d445@domainnotset.invalid> References: <5E6BFDF4F0AB2C4DA69CF4473FC7B948323307@wala01.seattlecca.org> <046201c5432f$4b565480$9f27d445@domainnotset.invalid> Message-ID: <6.0.0.22.1.20050418092535.01b1f058@gemini.msu.montana.edu> Following the thread here. One can purchase methyl violet free Methyl reen from Sigma. This avoids the messy procedure to remove methyl violet using toxic chloroform and a separating funnel inside a fume hood. We changed to Sigmas MG instead of taking a chance other companies methyl green dye lots were not MV free. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From jkiernan <@t> uwo.ca Mon Apr 18 10:41:52 2005 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] cryosections References: <1179.192.168.3.133.1113807290.squirrel@192.168.3.133> Message-ID: <4263D540.A4116B13@uwo.ca> If you and Prof.M.M.Panicke are molecular biologists with no prior experience of histological techniques, check out: http://www.portlandpress.com/pcs/books/prod_det.cfm?product=1855781565 Alternatively (probably better), hire a technician who has training, or at least some experience, in the kind of work to be done in your lab. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ ashokc@ncbs.res.in wrote: > > Hello > > Dear all > i need info about cryosections of mouse testis and staining,weather one > can use hemotoxylin& eosin for cryosections if so, what type of hematoxlin > is recommended. > > suggestions are well appreciated > > Ashok.C > Research Scholar > Prof.M.M.Panicker's lab > Molecular Neurobiology > National Centre for Biological Sciences > GKVK Campus > Bangalore-560065 > Phone-+91-080-23636421 ext :2251 > Fax no-91-080-23636662 From gcallis <@t> montana.edu Mon Apr 18 10:49:12 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:59 2005 Subject: H&E stain on Re: [Histonet] cryosections In-Reply-To: <1179.192.168.3.133.1113807290.squirrel@192.168.3.133> References: <1179.192.168.3.133.1113807290.squirrel@192.168.3.133> Message-ID: <6.0.0.22.1.20050418093617.01b7a008@gemini.msu.montana.edu> We do H&E on frozen sections frequently. Cut frozen sections, immerse them immediately into NBF, you can fix for 15 minutes or longer (have waited days or even even weeks before H&E) then stain with a basically routine paraffin section H&E protocol. We have not fixed with Bouins though. We do not air dry frozen sections destined for an H&E stain, they go directly to NBF. Be careful to do tap water rinsing gently - FS are more fragile, use Plus Charge slides. After fixation, rinse briefly to get rid of NBF and proceed with staining. Stain is a progressive hematoxylin, Gill II or Richard Allen Hematoxylin 1 for 1 1/2 minutes, rinse 1 minutes, dip 20 dips in Richard Allen Clarifier, a mild acetic acid solution, rinse in running tap water 1 min, bluing is either Scotts tap water substitute (30 sec) or Richard Allan bluing solution for 30 sec to 1 min, rinse with running tap water 1 min, 70% ethanol, eosin y for 30 seconds, dehydrate, clear and mount coverslip. You can probably use any hematoxylin you like as long as you adjust timing for your frozen sections and we avoid ammonia water for bluing. If your tap water is alkaline, you can rinse in warm tap water for 1 minutes for bluing also. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Terry.Marshall <@t> rothgen.nhs.uk Mon Apr 18 10:49:51 2005 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Temp/Humidity Records?[Scanned] Message-ID: Could you put that into English? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@elht.nhs.uk] Sent: 18 April 2005 15:20 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records?[Scanned] This differs from a scientific approach to a QMS to a non-scientific approach; a medical approach? Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Marshall Terry Dr,Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: 18 April 2005 13:40 To: Charles, Roger; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records?[Scanned] Grrrr. :-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Charles, Roger [mailto:rcharles@state.pa.us] Sent: 15 April 2005 17:54 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? This differs in being an accredited lab vs a non-accredited lab. -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Friday, April 15, 2005 11:45 AM To: Charles, Roger; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? This differs from being a waste of time and effort in what way? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Charles, Roger [mailto:rcharles@state.pa.us] Sent: 15 April 2005 16:36 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? We went thru this same ordeal a few years ago during our AAVLD inspection. I also had the same comments regarding the closed conditions of automated IHC and how room temp and humidity would have little or any affect on IHC. The inspector explained the requirement is justified when troubleshooting any testing that has somehow been transformed over time based on your control changes. The data would be used to rule out the room conditions more then to blame the room conditions as the cause. Our whole laboratory is now wired to monitor these conditions. Roger Charles TSE and IHC Technician Pa Veterinary Laboratory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela A. Marcum Sent: Friday, April 15, 2005 9:46 AM To: Marshall Terry Dr,Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu; Breeden,Sara Subject: RE: [Histonet] Temp/Humidity Records? The comment for the records were regarding IHC and yet I find changes in humidity and temperature have more effect on my sectioning and mounting of paraffin and plastic sections. If you are doing automated IHC I don't see how this would matter. If it is manual and you use a humidity chamber it still won't matter much. I could only see this mattering to IHC if it is done on the bench in open air. The changes I get seasons change throughout the day as heat or AC are added or changed. Is this really a new requirement?? Pam Marcum Quoting "Marshall Terry Dr, Consultant Histopathologist" : > So you keep accurate records. > What can you do with this information that is actually useful? > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] > Sent: 15 April 2005 14:30 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Temp/Humidity Records? > > > During a recent inspection, the SOP and documentation for room > temperature/humidity in the histo lab were noted as being absent. > Although I document instrument temperatures, I have not kept records on > room temp/humidity. It was noted that these factors may affect IHC > staining quality. If I am to begin documenting these parameters, I'd > like to know if there are any guidelines in a general sense (or a > specific one) relating to the variances and requirements. Any help > would be appreciated! > > Sara A. Breeden, HT(ASCP) > New Mexico Department of Agriculture > Veterinary Diagnostic Services > POBox 4700 > Albuquerque, NM 87196 > (505)841-2576 > (505)841-2518 FAX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Apr 18 11:08:15 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] IEC cryostat manual Message-ID: <200504181608.j3IG87OD016287@chip.viawest.net> I just inherited an IEC Minitome cryostat without a manual. Can anyone help find a manual, please? Patsy From jkiernan <@t> uwo.ca Mon Apr 18 12:15:31 2005 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Methyl green pyronin staining, methyl green source References: <5E6BFDF4F0AB2C4DA69CF4473FC7B948323307@wala01.seattlecca.org> <046201c5432f$4b565480$9f27d445@domainnotset.invalid> <6.0.0.22.1.20050418092535.01b1f058@gemini.msu.montana.edu> Message-ID: <4263EB33.7494C303@uwo.ca> The dye sold by Sigma is not methyl green. It is ethyl green, which is better. They call it methyl green on the label, but with the correct CI number (the one for ethyl green). Ethyl green can be found also under its right name in the Sigma catalog, with a cross-reference to the methyl green entry (which has ethyl green as a synonym, and the correct molecular formula for ethyl green). I don't know why they go on calling their ethyl green methyl green. The latter is a dye nobody would want these days because of its inevitable contamination with crystal violet. The CV originates during manufacture of MG and is also produced by spontaneous degradation in the stored dye powder. Ethyl green is made in a different way and it is also much more stable than methyl green. If anyone sells real methyl green, it must be from very old stock because that dye has not been manufactured for many (?30-40) years. See Floyd Green: Sigma-Aldrich Handbook of Stains, Dyes and Indicators (1991) for explanation of why ethyl green in not contaminated and does not change slowly into crystal violet. The differences between the two dyes are explained also in both the 9th and the 10th editions of Conn's Biological Stains. The staining method for DNA & RNA has, in reality, been ethyl green-pyronine for many years. Recognition of this fact in staining manuals and catalogs is long overdue. A standardized EGP method (Hoyer et al 1986 Histochem. J. 18:90-94) is technically simpler than MGP procedures that date from the days of real methyl green. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Gayle Callis wrote: > > Following the thread here. > > One can purchase methyl violet free Methyl reen from Sigma. This avoids > the messy procedure to remove methyl violet using toxic chloroform and a > separating funnel inside a fume hood. We changed to Sigmas MG instead of > taking a chance other companies methyl green dye lots were not MV free. > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From emry <@t> u.washington.edu Tue Apr 19 00:35:43 2005 From: emry <@t> u.washington.edu (Trisha Emry) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] EDTA Message-ID: I can buy two kinds of EDTA through my local university stores. One can be used for electrophoresis (Fisher BP 120-500 $27.38) and the other just listed as powder (Fisher S311-500 $36.37). With the great price difference (we have been using the $36.37 type) I was wondering if someone could tell me if there really is a difference and if I have to continue to use the pricey type. Thanks, Trisha U of Washington, Seattle From emry <@t> u.washington.edu Tue Apr 19 00:40:39 2005 From: emry <@t> u.washington.edu (Trisha Emry) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] snout Message-ID: We are decalcifying large 4x4x1 or 2 inch pieces of pig snout and the limits of my microtome make it impossible for me to cut. Are there any contract labs out there that can do the sectioning when we get them decalcified? Thanks, Trisha U of Washingtion, Seattle From steven.coakley <@t> mirusbio.com Mon Apr 18 13:23:30 2005 From: steven.coakley <@t> mirusbio.com (Steven Coakley) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] IHC fixation Message-ID: Inquiring about formalin substitutes for IHC in order to eliminate or decrease antigen retrieval. Steven From gcallis <@t> montana.edu Mon Apr 18 13:33:13 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] EDTA In-Reply-To: References: Message-ID: <6.0.0.22.1.20050418120752.01b4b1b0@gemini.msu.montana.edu> E sure to know which EDTA you are working with, there are several. EDTA disodium salt, it will work for decalcification and if only soluble at ~ 10 g/100 ml water with pH of 4 to 6 (5% solution) EDTA (no sodiums added) will work also. It is only soluble at ~10g/100 ml water, and will have pH of approx 4 to 6 (5% solution). EDTA tetrasodium salt is very soluble in buffers/water, as much as 14g/100 mls, but is very alkaline at pH 9, you must adjust the pH down and Webb Jee (very famous bone person) used acetic acid. This is the EDTA we prefer for bone work. Fisher catalog show no great differences between these two different grades of EDTA. (Fisher BP 120-500 $27.38) is electrophoresis grade, has been tested for lots of different things, found under Bioreagent listings and is the disodium salt. (Fisher S311-500 $36.37) is ACS certified found in analytical section of their catalog, extensively tested, and is the a disodium salt. It helps to keep a Fisher chemical catalog around to check out chemical purity, etc, etc. Either will work, go for the cheapest. If all you are doing is decalcification, then technical grade, even cheaper works just as well, the tetrasodium salt is technical grade. At 11:35 PM 4/18/2005, you wrote: >I can buy two kinds of EDTA through my local university stores. > >One can be used for electrophoresis (Fisher BP 120-500 $27.38) >and the other just listed as powder (Fisher S311-500 $36.37). > >With the great price difference (we have been using the $36.37 type) I was >wondering if someone could tell me if there really is a difference and if I >have to continue to use the pricey type. > >Thanks, >Trisha >U of Washington, Seattle > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From vazquezr <@t> ohsu.edu Mon Apr 18 13:34:46 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Microwave Processors Message-ID: Connie, Make sure it is a complete tissue processing microwave. We have a Ted Pella, which would be great except it doesn't have water loader and therefore it doesn't process properly. My department isn't willing to buy it. We are moving and will purchasing a tissue processor. Robyn OHSU From JMacDonald <@t> mtsac.edu Mon Apr 18 13:37:30 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] (no subject) In-Reply-To: <9BCAC308B27CAD4196D8AC9ADF037AAA110A79A9@wlmmsx01.nemours.org> Message-ID: Beginning in 2001, the ASCP added cytology preparation and staining to the HT exam. "Kristen Broomall" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/12/2005 02:20 PM To "'Shirley Powell'" , "'Gareth Davis'" , "'Shawn Leslie'" , "'Histonet'" cc Subject RE: [Histonet] (no subject) I can attest to that too. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shirley Powell Sent: Tuesday, April 12, 2005 5:16 PM To: 'Gareth Davis'; 'Shawn Leslie'; 'Histonet' Subject: RE: [Histonet] (no subject) A histotech I know took the HT last weekend here and told me there was a lot more cytology than expected on hers. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gareth Davis Sent: Tuesday, April 12, 2005 3:50 PM To: Shawn Leslie; Histonet Subject: Re: [Histonet] (no subject) Hi, I took the HT computer test last December. One helpful hint I can give is to know everything about tissue idenitification, what stain is needed for that tissue, what may go wrong with stain for a specific tissue and how to identify mistakes. I saw so many images, and it really caught me offguard. I expected many, but not as many as I saw and the questions to the images were not what I expected. I did pass with a good score, but I was really surprised that I did. Good luck. Gareth Davis --- Shawn Leslie wrote: > > > To All, > > I have a trainee who is about to sit for her HT computer exam, and > wondered if anyone has taken it recently, and could offer some helpful > hints. Thanks > > Shawn Leslie > > Confidentiality Notice: This e-mail message, including any > attachments, may contain confidential information. The information is > intended only for the use of the individual(s) or entity named above. > If you are not the intended recipient, you are notified that any > disclosure, copying, distribution, or the taking of any action in > reliance on the contents of this e-mail information is prohibited. > If you have received this e-mail in error, please contact the sender > by reply e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Apr 18 14:18:08 2005 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] neutral stains for protozoa In-Reply-To: Message-ID: There is an iron hematoxylin procedure for protozoa. It is in the AFIP manual. I don't have it. It is Heidens or something similar. Gary Radice Sent by: histonet-bounces@lists.utsouthwestern.edu 03/31/2005 06:47 AM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] neutral stains for protozoa I have a colleague who is studying a calcified protozoan. She is specifically interested in calcification, and she is looking for stains she can use that are not acidic and that don't require acid differentiation steps, so that she doesn't inadvertently decalcify the specimens. Any thoughts about nuclear or counterstains that might work in this situation? Gary P. Radice gradice@richmond.edu Department of Biology 804-289-8107 (voice) University of Richmond 804-289-8233 (FAX) Richmond VA 23173 http://www.richmond.edu/~gradice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Mon Apr 18 14:27:32 2005 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR)) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] neutral stains for protozoa Message-ID: <8F3AB322628548428A992EFB0E80F5D315D630D6@nihexchange8.nih.gov> You can stain with the Von Kossa with a nuclear fast red counterstain. Ruth Yaskovich N.I.H. > ---------- > From: Jennifer MacDonald > Sent: Monday, April 18, 2005 2:18 PM > To: Gary Radice > Cc: Histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] neutral stains for protozoa > > There is an iron hematoxylin procedure for protozoa. It is in the AFIP > manual. I don't have it. It is Heidens or something similar. > > > > > > > Gary Radice > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/31/2005 06:47 AM > > To > Histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] neutral stains for protozoa > > > > > > > I have a colleague who is studying a calcified protozoan. She is > specifically interested in calcification, and she is looking for stains > she can use that are not acidic and that don't require acid > differentiation steps, so that she doesn't inadvertently decalcify the > specimens. > > Any thoughts about nuclear or counterstains that might work in this > situation? > > > > Gary P. Radice gradice@richmond.edu > Department of Biology 804-289-8107 (voice) > University of Richmond 804-289-8233 (FAX) > Richmond VA 23173 http://www.richmond.edu/~gradice > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From TJJ <@t> Stowers-Institute.org Mon Apr 18 14:48:00 2005 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Paraffin processing late stage mouse embryos Message-ID: Is anybody doing paraffin processing on E18.5 - P0 mouse embryos that can help us optimize our in-house protocol? I know that when possible one should bisect embryos for best fixation and processing. Unfortunately we can't always do that. In some instances, we are able to bisect the samples just posterior to the forelimbs and process only the top 1/2 of the embryo. Even after poking some holes and immersion for 72 hours in 10% NBF, combined with a rigorous processing schedule (2 hours/station with a typical automated tissue processor setup--same schedule as we use for decalcified whole adult mouse heads!), our samples just aren't sectioning well, and they tend to blow up on the water bath. A test with samples without holes poked in them vs. ones with holes produced no discernable improvement in processing. A google search turned up one lab (evidently hand processing) who recommends removing the skin of this size embryo (E15.5 - E18.5) and removing the head and/or limbs if not needed for study. Fixation and processing: fix 1 hour @ 40 degrees C, change fixative, then 40 degrees C overnight. The following are done at room temp or refrigerated for IHC or ISH: dehydrating in 30% ETOH 1-2 hrs, 50% ETOH 1-2 hrs, 70% ETOH 4 hrs or O/N, 95% ETOH 3 hrs x 2 or O/N, and 100% ETOH 1 hr x 2. Embryos can be stored at -20 degrees C at this point. Carrying on with dehydration and infiltration: 100% ETOH 1 hr x 3 @ RT, Xylene 30-40 min x 2 (check embryos*), paraffin 40 minutes x 3, paraffin embedding. *No indication of what we're actually checking on these samples I do think that removing the skin on these guys would help immensely. I also think that additional dissection when possible is critical to good fixation and processing. Any comments on the above processing schedule? Is anybody doing these later stage mouse embryo samples using a protocol that's working for you? Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org From cwscouten <@t> myneurolab.com Mon Apr 18 15:13:49 2005 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Cryostat Message-ID: <5784D843593D874C93E9BADCB87342AB44F87C@tpiserver03.Coretech-holdings.com> See the following link: This works great. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=475102&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats&idsubcategory=182 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Monday, April 18, 2005 8:14 AM To: Histonet (E-mail) Subject: [Histonet] Cryostat All, I'm looking for a reliable cryostat with self disinfection. Any suggestion? Thanks Bruce Chung, MSM, HT/CT(ASCP) Anatomic Pathology Manager Phoebe Putney Memorial Hospital lchung@ppmh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From matt <@t> ategra.com Mon Apr 18 16:03:51 2005 From: matt <@t> ategra.com (Matt Hawes (extension 224)) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] New Histology and MOH's Technologist Job Opportunities Message-ID: Hello Histonetters, My name is Matt Hawes and I am the Senior AP Recruiter here at Ategra Systems and I have received new Histology and MOH's Technologist Job Opportunities that I wanted to inform you about and see if you might be interested or if you might happen to know of anyone looking for new employment opportunities? Here are some of my HOTTEST Fulltime Permanent Histo Tech positions & MOH's Tech opportunities: 1. Boston Burbs, MA- MOH's/ Histo Tech- Day Shift 2. Rockville, MD- MOH's/Histo Tech- Day Shift 3. Northeastern MI- Histo Tech-Day Shift 4. Sioux City, IA- Histo Tech-Day Shift 5. Los Angeles, CA- IHC/Hist Supervisor 6. Rhode Island- Histo Tech-Day Shift 7. New Jersey-Histo Tech- Nite Shift (3 openings) 8. Upstate NY- Histo Tech- Day Shift (2 openings) These positions are as direct employees of our clients. They all offer full employee benefits (if working in a fulltime capacity): excellent healthcare, life insurance, dental, vision, 401K/retirement - as well as relocation bonuses (where applic). If you are interested in these jobs, please CALL ME ASAP at (800)466 9919 x224 or e-mail me at Matt@ategra.com . To speed things up, please also send me a copy of your resume, (if you haven't already done so). If you are interested in jobs outside the above-mentioned areas, please send me your resume as well as I have clients throughout the US. I will keep your resume confidential and will not release it to anyone without your permission (This is Ategra policy as well as my own). My services are at no charge to you. Of course, you may be happy in your present job, but it never hurts to to keep an eye open. Also, if you have friends/peers who might be interested as well, if you could pass my query & name on to them I'd be very grateful. However, if you are interested in any of the jobs above, please call me. Thank You !! Matt Hawes Senior Laboratory Recruiter Ategra Systems (800) 466-9919 Ext 234 Matt@Ategra.com --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing VOICE: 407-671-5800 ext 224 TOLLFREE: 800-466-9919 Ext 224 Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu To Learn More About Ategra: http://www.ategra.com -------------------------------------------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. -------------------------------------------------------------------------------------------------- From steven.coakley <@t> mirusbio.com Tue Apr 19 08:41:41 2005 From: steven.coakley <@t> mirusbio.com (Steven Coakley) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Qc forms Message-ID: I am starting up a histo lab and in need of varies qc forms. I got a few from Carsons. Any would be appreciated. Email me at sjchtascp@yahoo.com Thanks From tflore <@t> lsuhsc.edu Tue Apr 19 08:49:03 2005 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] QC & QA's Message-ID: I know I have seen them, but is there a site for Histotechnology QC and QA on sectioning techniques, staining techniques and quality, etc? Teresa Flores LSUHSC NO, LA From pruegg <@t> ihctech.net Tue Apr 19 11:56:21 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] neutral stains for protozoa In-Reply-To: <8F3AB322628548428A992EFB0E80F5D315D630D6@nihexchange8.nih.gov> Message-ID: <200504191656.j3JGuCOD021472@chip.viawest.net> I use a H&E counterstain for Von Kossa. I use gills 3 hematoxylin and do not destain in acid alcohol/ I do blue briefly in ammonia water, wash well and then use a 0.2% aqueous eosin for 30 min., dehydrate quickly thru 95% alcohols, 100 and xylene then permount. This method will stain osteoid on bone viewed by fluorescence UV. It is very nice for histomorphometry by image analysis as only the osteoid lights up and the rest of the tissue (calcified bone and blue nuclei) is dark under UV, makes it really easy to measure osteoid seams. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) Sent: Monday, April 18, 2005 12:28 PM To: Gary Radice; 'Jennifer MacDonald' Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] neutral stains for protozoa You can stain with the Von Kossa with a nuclear fast red counterstain. Ruth Yaskovich N.I.H. > ---------- > From: Jennifer MacDonald > Sent: Monday, April 18, 2005 2:18 PM > To: Gary Radice > Cc: Histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] neutral stains for protozoa > > There is an iron hematoxylin procedure for protozoa. It is in the > AFIP manual. I don't have it. It is Heidens or something similar. > > > > > > > Gary Radice > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/31/2005 06:47 AM > > To > Histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] neutral stains for protozoa > > > > > > > I have a colleague who is studying a calcified protozoan. She is > specifically interested in calcification, and she is looking for > stains she can use that are not acidic and that don't require acid > differentiation steps, so that she doesn't inadvertently decalcify the > specimens. > > Any thoughts about nuclear or counterstains that might work in this > situation? > > > > Gary P. Radice gradice@richmond.edu > Department of Biology 804-289-8107 (voice) > University of Richmond 804-289-8233 (FAX) > Richmond VA 23173 http://www.richmond.edu/~gradice > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmeyers <@t> ctcsurge.com Tue Apr 19 12:17:13 2005 From: rmeyers <@t> ctcsurge.com (Roger Meyers) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] automated response Message-ID: <10504191317.AA21413102@mail.ctcsurge.com> I will be out of the office and unavailable Tuesday, 4/19/05. If you need immediate assistance, please contact Laura Beaudet at 617-557-9264. From alan <@t> ptech.co.nz Tue Apr 19 13:10:54 2005 From: alan <@t> ptech.co.nz (Alan Peacock) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Differentiating brain tissue In-Reply-To: <20050419170703.D101648214@sif.iconz.co.nz> Message-ID: <4.2.0.58.20050420060816.00bade40@mail.iconz.co.nz> Hi, Does anyone know of a procedure or stain that can differentiate between human brain tissue and animal brain tissue on a fabric? Thanks, lan Peacock B.Sc From mcauliff <@t> umdnj.edu Tue Apr 19 16:22:41 2005 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Differentiating brain tissue In-Reply-To: <4.2.0.58.20050420060816.00bade40@mail.iconz.co.nz> References: <4.2.0.58.20050420060816.00bade40@mail.iconz.co.nz> Message-ID: <426576A1.3020300@umdnj.edu> I would hire a good forensics lab. Geoff Alan Peacock wrote: > > Hi, > > Does anyone know of a procedure or stain that can differentiate > between human brain tissue and animal brain tissue on a fabric? > > Thanks, > lan Peacock B.Sc > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From themagoos <@t> rushmore.com Tue Apr 19 14:39:37 2005 From: themagoos <@t> rushmore.com (Jason and Heather) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Fungus Control References: <4.2.0.58.20050420060816.00bade40@mail.iconz.co.nz> <426576A1.3020300@umdnj.edu> Message-ID: <000e01c54517$87344040$b3fc9fd1@magoo> My lab is in need of fungus control. We are willing to trade AFB control for fungus. I guess we live in a fungus free enviroment in South Dakota. If anybody can help please let me know. Jason McGough HT Clinical Laboratory of the Black Hills 2805 5th St. Rapid City, SD 57701 605-343-2267 themagoos@rushmore.com From CCollins <@t> propathlab.com Tue Apr 19 14:50:39 2005 From: CCollins <@t> propathlab.com (Cory Collins) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Immunofluorescence on salt-split skin Message-ID: I found out today that my lab will be doing IF's on salt-split skin biopsies in the near future for bullous pemphigoid. Can anyone that is currently doing this give me some insight into the sodium chloride concentration, the temperature, and the amount of time you leave the skin in the solution? Also, does the skin sperate on its own or do you have to use forceps to seperate the dermis from the epidermis? Thanks, Cory Cory Collins, HT (ASCP) QIHC Immunohistochemistry Supervisor ProPath 8267 Elmbrook Drive Suite 100 Dallas, TX 75247 214-638-2000 ext 2027 214-237-1730 Fax To learn more about ProPath, please visit http://www.ProPathLab.com ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From nmuvarak <@t> facstaff.wisc.edu Tue Apr 19 15:26:50 2005 From: nmuvarak <@t> facstaff.wisc.edu (NIDAL E MUVARAK) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Stain for Proteoglycans Message-ID: <9d225e9d3506.9d35069d225e@wiscmail.wisc.edu> Is there a stain we could do to assess proteoglycan content in the mouse pulmonary arteries? From vazquezr <@t> ohsu.edu Tue Apr 19 15:53:53 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Immunofluorescence on salt-split skin Message-ID: Cory, I can fax our recipe and procedure to you. Robyn OHSU From colesy1222 <@t> yahoo.com Tue Apr 19 16:24:39 2005 From: colesy1222 <@t> yahoo.com (Nicole Puglisi) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Digital Imaging Message-ID: <20050419212439.72580.qmail@web30605.mail.mud.yahoo.com> I'm looking to get some information on digital imaging software. If anyone is currently using digital imaging in their lab, would you please let me know what software you are currently using. I know there is a lot of software out there. I'm trying to weed out software for imaging in our lab. If you are using digital imaging are you happy with the software you have? Any help would be greatly appreciated. Thanks! Nicole Puglisi __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From AGrobe2555 <@t> aol.com Tue Apr 19 16:43:52 2005 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] (no subject) Message-ID: <1f3.8125b65.2f96d598@aol.com> Hello Folks, I wonder if anyone has used DCFDA on cryosections to quantify hydrogen peroxide in tissues. I have found references that use this with intact cells but not tissues. Any help is appreciated. Albert From cfavara <@t> niaid.nih.gov Tue Apr 19 18:31:36 2005 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Paraffin processing late stage mouse embryos Message-ID: Teri, I do a lot of work with mouse tissue and I find that if the tissue is blowing up on the water bath that most likely there is inadequate paraffin infiltration, which may be a result of inadequate fixation. If the staining you are doing can stand a longer time in fixative I would recommend a week in NBF making sure you have an adequate volume. I would try a gentler processing schedule. 70%ETOH 3 hours, 80% ETOH 1 hour, 95% ETOH 2 changes 20 minutes each, 100% ETOH 2 changes each, Propar 3 changes 30 min each, 4 paraffin 4 changes 30 minutes each. I personally prefer Propar to Xylene for mouse tissue and four paraffin baths. Hope this is helpful. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Johnson, Teri [mailto:TJJ@Stowers-Institute.org] Sent: Monday, April 18, 2005 12:48 PM To: Histonet Subject: [Histonet] Paraffin processing late stage mouse embryos Is anybody doing paraffin processing on E18.5 - P0 mouse embryos that can help us optimize our in-house protocol? I know that when possible one should bisect embryos for best fixation and processing. Unfortunately we can't always do that. In some instances, we are able to bisect the samples just posterior to the forelimbs and process only the top 1/2 of the embryo. Even after poking some holes and immersion for 72 hours in 10% NBF, combined with a rigorous processing schedule (2 hours/station with a typical automated tissue processor setup--same schedule as we use for decalcified whole adult mouse heads!), our samples just aren't sectioning well, and they tend to blow up on the water bath. A test with samples without holes poked in them vs. ones with holes produced no discernable improvement in processing. A google search turned up one lab (evidently hand processing) who recommends removing the skin of this size embryo (E15.5 - E18.5) and removing the head and/or limbs if not needed for study. Fixation and processing: fix 1 hour @ 40 degrees C, change fixative, then 40 degrees C overnight. The following are done at room temp or refrigerated for IHC or ISH: dehydrating in 30% ETOH 1-2 hrs, 50% ETOH 1-2 hrs, 70% ETOH 4 hrs or O/N, 95% ETOH 3 hrs x 2 or O/N, and 100% ETOH 1 hr x 2. Embryos can be stored at -20 degrees C at this point. Carrying on with dehydration and infiltration: 100% ETOH 1 hr x 3 @ RT, Xylene 30-40 min x 2 (check embryos*), paraffin 40 minutes x 3, paraffin embedding. *No indication of what we're actually checking on these samples I do think that removing the skin on these guys would help immensely. I also think that additional dissection when possible is critical to good fixation and processing. Any comments on the above processing schedule? Is anybody doing these later stage mouse embryo samples using a protocol that's working for you? Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Tue Apr 19 21:10:54 2005 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Stain for Proteoglycans Message-ID: The classic stain for proeoglycans is the Movatt's stain in the AFIP ( insert proteoglycans for ground substances), though I have found this stain to be quite long and requires stains not routinely used in a modern laboratory. We reularly use a modification of this method (using common stains used for other methods) for the staining of human aorta to demonstate proteoglycans which are the result of degenration of the connective tissue eg Marfan's syndrome. First stain with Alcian Blue in the usual manner. Fix this stain using the alcoholic ammonia as in Movatt's. Then perform a standard Verhoeff's van Gieson. This will give you the blue staining of the proteoglycans and classic staining of elastin fibres and collagen with the VVG. The value of the method over a routine connective tissue stain can be seen in cases where the proteoglycans are present even though the connective tisssue staining appears normal. The critical step is the alcoholic ammonia which transforms the Alcian Blue to insoluable Monastral Blue without which all blue staining would be lost. All the best. Do not hesitate to contact me if you want a detailed version of the method. Tony Reilly Chief Scientist Anatomical Pathology Northside Pathology Prince Charles Hospital Ph: 3350 8543 Fax: 33508546 Email: tony_reilly@health.qld.gov.au >>> NIDAL E MUVARAK 04/20/05 06:26am >>> Is there a stain we could do to assess proteoglycan content in the mouse pulmonary arteries? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *********************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is prohibited. It may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone or by return email. You should also delete this email and destroy any hard copies produced. *********************************************************************************** From RCHIOVETTI <@t> aol.com Tue Apr 19 22:04:48 2005 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Seeking Histotechs' and Pathologists' Input Message-ID: <157.4f260f06.2f9720d0@aol.com> Fellow Histonetters, I'm looking for a few good histotechs and pathologists! More accurately, I'm looking for input and opinions in the areas of cytology, histology, histotechnology, day-to-day operations in the lab, how decisions on equipment and instrumentation get made, what the liines of authority are in various labs, etc. This information is for a client of mine who would like to get a general idea about lab practices, procedures and operations. It's important that we get input from the perspectives of both the pathologist and the histotechnologist. We welcome participation from academic labs, private and public hospitals, and reference labs. The more, the merrier! If you and/or your docs are willing to participate in a 15-minute telephone call, please contact me off-list for more info or send me the contact information. I promise, this is a one-shot deal and it will only take an investment of 15 minutes. Thanks in advance for any assistance you can provide.. Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Independent Consultant for The Science, Technology and Industrial Sectors 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 rchiovetti@aol.com Member, Arizona Small Business Association - ASBA From Kemlo.Rogerson <@t> elht.nhs.uk Wed Apr 20 01:55:31 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Differentiating brain tissue[Scanned] Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F1D5@bhrv-nt-11.bhrv.nwest.nhs.uk> What an interesting question; it would depend on the type of DNA wouldn't it, or the number of neurones. Wonder if you could the difference between a Manchester United fan and a human? Just joking, Blackburn Rovers would have been too near home. -----Original Message----- From: Alan Peacock [mailto:alan@ptech.co.nz] Sent: 19 April 2005 19:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Differentiating brain tissue[Scanned] Hi, Does anyone know of a procedure or stain that can differentiate between human brain tissue and animal brain tissue on a fabric? Thanks, lan Peacock B.Sc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Wed Apr 20 02:47:32 2005 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] woe is me Message-ID: Hi all, anyone know how I can rescue a whole bunch of samples that polymerised into one huge mass in MMA? I have acess to all those great but toxic solvents such as chloroform, acetone, toluene and xylene. (naturally I use these under appropriate safety conditions, such as when smoking and eating my lunch ;o) ) thank you in advance -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "....I know who I am. No-one else knows who I am. If I was a giraffe, and someone said I was a snake, I'd think no, actually I'm a giraffe" Richard Gere From jnocito <@t> satx.rr.com Wed Apr 20 05:55:05 2005 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] woe is me References: Message-ID: <002b01c54597$6ad5ec70$7929f318@yourxhtr8hvc4p> can't help you, but I do have extra osmium tetroxide to sprinkle on your sandwich for that added flavor. Sorry, couldn't resist. I've been too quiet lately. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "louise renton" To: Sent: Wednesday, April 20, 2005 2:47 AM Subject: [Histonet] woe is me Hi all, anyone know how I can rescue a whole bunch of samples that polymerised into one huge mass in MMA? I have acess to all those great but toxic solvents such as chloroform, acetone, toluene and xylene. (naturally I use these under appropriate safety conditions, such as when smoking and eating my lunch ;o) ) thank you in advance -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "....I know who I am. No-one else knows who I am. If I was a giraffe, and someone said I was a snake, I'd think no, actually I'm a giraffe" Richard Gere _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Anti-Virus. Version: 7.0.308 / Virus Database: 266.9.17 - Release Date: 4/19/2005 From TMcNemar <@t> lmhealth.org Wed Apr 20 06:09:53 2005 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Fungus Control Message-ID: <6CD94D97ED7D924BA5C2B588FA9528213967B2@nt_exchange> I could could swap a couple with you. I grew my own using bread mold on a blood agar plate. Once it grows, cut it up and process the pieces. Works very well. Let me know (email below). Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio 43055 tmcnemar@lmhealth.org -----Original Message----- From: Jason and Heather [mailto:themagoos@rushmore.com] Sent: Tuesday, April 19, 2005 3:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fungus Control My lab is in need of fungus control. We are willing to trade AFB control for fungus. I guess we live in a fungus free enviroment in South Dakota. If anybody can help please let me know. Jason McGough HT Clinical Laboratory of the Black Hills 2805 5th St. Rapid City, SD 57701 605-343-2267 themagoos@rushmore.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lnicker1 <@t> tampabay.rr.com Wed Apr 20 09:32:57 2005 From: lnicker1 <@t> tampabay.rr.com (lnicker1) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] reply Message-ID: <001001c545b5$d8eff9d0$6401a8c0@Lola> From dahmed <@t> mdanderson.org Wed Apr 20 10:14:40 2005 From: dahmed <@t> mdanderson.org (dahmed@mdanderson.org) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] (no subject) Message-ID: Does anyone know where I can purchase an inexpensive low speed agitation plate? I need one that is fairly large for specimen container agitation? David S. Ahmed Chief Histology Technician Department of Pathology M.D. Anderson Cancer Center From Heather.A.Harper <@t> pcola.med.navy.mil Wed Apr 20 10:35:52 2005 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Need info for Message-ID: <807FE48C5A7CC940B973B58D32E70143194CD5E5@nhpens-exch1.pcola.med.navy.mil> Hi everyone, I'm looking for information for techs who work for any VA hospitals and Naval Hospitals, mainly in the state of Florida, but also across the nation. I need to know if that person grosses and what GS level they started at prior to grossing and what current GS position they hold. Also would like someone to share their work PD description, and possibly send me a copy. I am currently learning to gross but believe that most government techs are a GS 8, who gross. I am in the process of writing a new work PD, and my current work PD doesn't have an adequate description of my histology duties. Thank you in advance. Heather A. Harper Supervisor of Histology/Morgue Naval Hospital of Pensacola, FL. From mclarke <@t> allsaintshealthcare.org Wed Apr 20 10:46:33 2005 From: mclarke <@t> allsaintshealthcare.org (Clarke, Mary) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] slide dryer Message-ID: <3959B2255CA51443928A90517C973D6418EC6A@WFEXBE04.wfsi.priv> I have an old slide dryer from Lipshaw that is dying and wondering if anyone knows where I can get a replacement. It measures about 10x7x5 inches. The model number is 218. Thanks in advance for your help. Terri Clarke Histology Supervisor All Saints Laboratory 3801 Spring Street Racine, Wi 53405 mclarke@allsaintshealthcare.org 262-687-6609 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, April 19, 2005 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 17, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Methyl green pyronin staining, methyl green source (John A. Kiernan) 2. EDTA (Trisha Emry) 3. snout (Trisha Emry) 4. IHC fixation (Steven Coakley) 5. Re: EDTA (Gayle Callis) 6. Re: Microwave Processors (Robyn Vazquez) 7. RE: (no subject) (Jennifer MacDonald) 8. Re: neutral stains for protozoa (Jennifer MacDonald) 9. RE: neutral stains for protozoa (Yaskovich, Ruth A (NIH/NIDCR)) 10. Paraffin processing late stage mouse embryos (Johnson, Teri) 11. RE: Cryostat (Charles Scouten) 12. New Histology and MOH's Technologist Job Opportunities (Matt Hawes (extension 224)) 13. Qc forms (Steven Coakley) 14. QC & QA's (Flores, Teresa) 15. RE: neutral stains for protozoa (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Mon, 18 Apr 2005 13:15:31 -0400 From: "John A. Kiernan" Subject: Re: [Histonet] Methyl green pyronin staining, methyl green source To: Gayle Callis Cc: Histonet@lists.utsouthwestern.edu Message-ID: <4263EB33.7494C303@uwo.ca> Content-Type: text/plain; charset=us-ascii The dye sold by Sigma is not methyl green. It is ethyl green, which is better. They call it methyl green on the label, but with the correct CI number (the one for ethyl green). Ethyl green can be found also under its right name in the Sigma catalog, with a cross-reference to the methyl green entry (which has ethyl green as a synonym, and the correct molecular formula for ethyl green). I don't know why they go on calling their ethyl green methyl green. The latter is a dye nobody would want these days because of its inevitable contamination with crystal violet. The CV originates during manufacture of MG and is also produced by spontaneous degradation in the stored dye powder. Ethyl green is made in a different way and it is also much more stable than methyl green. If anyone sells real methyl green, it must be from very old stock because that dye has not been manufactured for many (?30-40) years. See Floyd Green: Sigma-Aldrich Handbook of Stains, Dyes and Indicators (1991) for explanation of why ethyl green in not contaminated and does not change slowly into crystal violet. The differences between the two dyes are explained also in both the 9th and the 10th editions of Conn's Biological Stains. The staining method for DNA & RNA has, in reality, been ethyl green-pyronine for many years. Recognition of this fact in staining manuals and catalogs is long overdue. A standardized EGP method (Hoyer et al 1986 Histochem. J. 18:90-94) is technically simpler than MGP procedures that date from the days of real methyl green. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Gayle Callis wrote: > > Following the thread here. > > One can purchase methyl violet free Methyl reen from Sigma. This avoids > the messy procedure to remove methyl violet using toxic chloroform and a > separating funnel inside a fume hood. We changed to Sigmas MG instead of > taking a chance other companies methyl green dye lots were not MV free. > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 18 Apr 2005 22:35:43 -0700 From: "Trisha Emry" Subject: [Histonet] EDTA To: "histo" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I can buy two kinds of EDTA through my local university stores. One can be used for electrophoresis (Fisher BP 120-500 $27.38) and the other just listed as powder (Fisher S311-500 $36.37). With the great price difference (we have been using the $36.37 type) I was wondering if someone could tell me if there really is a difference and if I have to continue to use the pricey type. Thanks, Trisha U of Washington, Seattle ------------------------------ Message: 3 Date: Mon, 18 Apr 2005 22:40:39 -0700 From: "Trisha Emry" Subject: [Histonet] snout To: "Histonet@lists. utsouthwestern. edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We are decalcifying large 4x4x1 or 2 inch pieces of pig snout and the limits of my microtome make it impossible for me to cut. Are there any contract labs out there that can do the sectioning when we get them decalcified? Thanks, Trisha U of Washingtion, Seattle ------------------------------ Message: 4 Date: Mon, 18 Apr 2005 13:23:30 -0500 From: "Steven Coakley" Subject: [Histonet] IHC fixation To: Message-ID: Content-Type: text/plain; charset="us-ascii" Inquiring about formalin substitutes for IHC in order to eliminate or decrease antigen retrieval. Steven ------------------------------ Message: 5 Date: Mon, 18 Apr 2005 12:33:13 -0600 From: Gayle Callis Subject: Re: [Histonet] EDTA To: "Trisha Emry" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050418120752.01b4b1b0@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed E sure to know which EDTA you are working with, there are several. EDTA disodium salt, it will work for decalcification and if only soluble at ~ 10 g/100 ml water with pH of 4 to 6 (5% solution) EDTA (no sodiums added) will work also. It is only soluble at ~10g/100 ml water, and will have pH of approx 4 to 6 (5% solution). EDTA tetrasodium salt is very soluble in buffers/water, as much as 14g/100 mls, but is very alkaline at pH 9, you must adjust the pH down and Webb Jee (very famous bone person) used acetic acid. This is the EDTA we prefer for bone work. Fisher catalog show no great differences between these two different grades of EDTA. (Fisher BP 120-500 $27.38) is electrophoresis grade, has been tested for lots of different things, found under Bioreagent listings and is the disodium salt. (Fisher S311-500 $36.37) is ACS certified found in analytical section of their catalog, extensively tested, and is the a disodium salt. It helps to keep a Fisher chemical catalog around to check out chemical purity, etc, etc. Either will work, go for the cheapest. If all you are doing is decalcification, then technical grade, even cheaper works just as well, the tetrasodium salt is technical grade. At 11:35 PM 4/18/2005, you wrote: >I can buy two kinds of EDTA through my local university stores. > >One can be used for electrophoresis (Fisher BP 120-500 $27.38) >and the other just listed as powder (Fisher S311-500 $36.37). > >With the great price difference (we have been using the $36.37 type) I was >wondering if someone could tell me if there really is a difference and if I >have to continue to use the pricey type. > >Thanks, >Trisha >U of Washington, Seattle > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 6 Date: Mon, 18 Apr 2005 11:34:46 -0700 From: "Robyn Vazquez" Subject: Re: [Histonet] Microwave Processors To: conniegrubaugh@hotmail.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Connie, Make sure it is a complete tissue processing microwave. We have a Ted Pella, which would be great except it doesn't have water loader and therefore it doesn't process properly. My department isn't willing to buy it. We are moving and will purchasing a tissue processor. Robyn OHSU ------------------------------ Message: 7 Date: Mon, 18 Apr 2005 11:37:30 -0700 From: Jennifer MacDonald Subject: RE: [Histonet] (no subject) To: "Kristen Broomall" Cc: 'Histonet' , 'Shawn Leslie' , histonet-bounces@lists.utsouthwestern.edu, 'Shirley Powell' , 'Gareth Davis' Message-ID: Content-Type: text/plain; charset="US-ASCII" Beginning in 2001, the ASCP added cytology preparation and staining to the HT exam. "Kristen Broomall" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/12/2005 02:20 PM To "'Shirley Powell'" , "'Gareth Davis'" , "'Shawn Leslie'" , "'Histonet'" cc Subject RE: [Histonet] (no subject) I can attest to that too. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shirley Powell Sent: Tuesday, April 12, 2005 5:16 PM To: 'Gareth Davis'; 'Shawn Leslie'; 'Histonet' Subject: RE: [Histonet] (no subject) A histotech I know took the HT last weekend here and told me there was a lot more cytology than expected on hers. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gareth Davis Sent: Tuesday, April 12, 2005 3:50 PM To: Shawn Leslie; Histonet Subject: Re: [Histonet] (no subject) Hi, I took the HT computer test last December. One helpful hint I can give is to know everything about tissue idenitification, what stain is needed for that tissue, what may go wrong with stain for a specific tissue and how to identify mistakes. I saw so many images, and it really caught me offguard. I expected many, but not as many as I saw and the questions to the images were not what I expected. I did pass with a good score, but I was really surprised that I did. Good luck. Gareth Davis --- Shawn Leslie wrote: > > > To All, > > I have a trainee who is about to sit for her HT computer exam, and > wondered if anyone has taken it recently, and could offer some helpful > hints. Thanks > > Shawn Leslie > > Confidentiality Notice: This e-mail message, including any > attachments, may contain confidential information. The information is > intended only for the use of the individual(s) or entity named above. > If you are not the intended recipient, you are notified that any > disclosure, copying, distribution, or the taking of any action in > reliance on the contents of this e-mail information is prohibited. > If you have received this e-mail in error, please contact the sender > by reply e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 18 Apr 2005 12:18:08 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] neutral stains for protozoa To: Gary Radice Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" There is an iron hematoxylin procedure for protozoa. It is in the AFIP manual. I don't have it. It is Heidens or something similar. Gary Radice Sent by: histonet-bounces@lists.utsouthwestern.edu 03/31/2005 06:47 AM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] neutral stains for protozoa I have a colleague who is studying a calcified protozoan. She is specifically interested in calcification, and she is looking for stains she can use that are not acidic and that don't require acid differentiation steps, so that she doesn't inadvertently decalcify the specimens. Any thoughts about nuclear or counterstains that might work in this situation? Gary P. Radice gradice@richmond.edu Department of Biology 804-289-8107 (voice) University of Richmond 804-289-8233 (FAX) Richmond VA 23173 http://www.richmond.edu/~gradice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 18 Apr 2005 15:27:32 -0400 From: "Yaskovich, Ruth A (NIH/NIDCR)" Subject: RE: [Histonet] neutral stains for protozoa To: Gary Radice , 'Jennifer MacDonald' Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: <8F3AB322628548428A992EFB0E80F5D315D630D6@nihexchange8.nih.gov> Content-Type: text/plain; charset="iso-8859-1" You can stain with the Von Kossa with a nuclear fast red counterstain. Ruth Yaskovich N.I.H. > ---------- > From: Jennifer MacDonald > Sent: Monday, April 18, 2005 2:18 PM > To: Gary Radice > Cc: Histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] neutral stains for protozoa > > There is an iron hematoxylin procedure for protozoa. It is in the AFIP > manual. I don't have it. It is Heidens or something similar. > > > > > > > Gary Radice > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/31/2005 06:47 AM > > To > Histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] neutral stains for protozoa > > > > > > > I have a colleague who is studying a calcified protozoan. She is > specifically interested in calcification, and she is looking for stains > she can use that are not acidic and that don't require acid > differentiation steps, so that she doesn't inadvertently decalcify the > specimens. > > Any thoughts about nuclear or counterstains that might work in this > situation? > > > > Gary P. Radice gradice@richmond.edu > Department of Biology 804-289-8107 (voice) > University of Richmond 804-289-8233 (FAX) > Richmond VA 23173 http://www.richmond.edu/~gradice > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 10 Date: Mon, 18 Apr 2005 14:48:00 -0500 From: "Johnson, Teri" Subject: [Histonet] Paraffin processing late stage mouse embryos To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Is anybody doing paraffin processing on E18.5 - P0 mouse embryos that can help us optimize our in-house protocol? I know that when possible one should bisect embryos for best fixation and processing. Unfortunately we can't always do that. In some instances, we are able to bisect the samples just posterior to the forelimbs and process only the top 1/2 of the embryo. Even after poking some holes and immersion for 72 hours in 10% NBF, combined with a rigorous processing schedule (2 hours/station with a typical automated tissue processor setup--same schedule as we use for decalcified whole adult mouse heads!), our samples just aren't sectioning well, and they tend to blow up on the water bath. A test with samples without holes poked in them vs. ones with holes produced no discernable improvement in processing. A google search turned up one lab (evidently hand processing) who recommends removing the skin of this size embryo (E15.5 - E18.5) and removing the head and/or limbs if not needed for study. Fixation and processing: fix 1 hour @ 40 degrees C, change fixative, then 40 degrees C overnight. The following are done at room temp or refrigerated for IHC or ISH: dehydrating in 30% ETOH 1-2 hrs, 50% ETOH 1-2 hrs, 70% ETOH 4 hrs or O/N, 95% ETOH 3 hrs x 2 or O/N, and 100% ETOH 1 hr x 2. Embryos can be stored at -20 degrees C at this point. Carrying on with dehydration and infiltration: 100% ETOH 1 hr x 3 @ RT, Xylene 30-40 min x 2 (check embryos*), paraffin 40 minutes x 3, paraffin embedding. *No indication of what we're actually checking on these samples I do think that removing the skin on these guys would help immensely. I also think that additional dissection when possible is critical to good fixation and processing. Any comments on the above processing schedule? Is anybody doing these later stage mouse embryo samples using a protocol that's working for you? Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org ------------------------------ Message: 11 Date: Mon, 18 Apr 2005 15:13:49 -0500 From: "Charles Scouten" Subject: RE: [Histonet] Cryostat To: "Chung, Luong" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <5784D843593D874C93E9BADCB87342AB44F87C@tpiserver03.Coretech-holdings.com> Content-Type: text/plain; charset="iso-8859-1" See the following link: This works great. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=475102&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats&idsubcategory=182 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Monday, April 18, 2005 8:14 AM To: Histonet (E-mail) Subject: [Histonet] Cryostat All, I'm looking for a reliable cryostat with self disinfection. Any suggestion? Thanks Bruce Chung, MSM, HT/CT(ASCP) Anatomic Pathology Manager Phoebe Putney Memorial Hospital lchung@ppmh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 18 Apr 2005 17:03:51 -0400 From: Matt Hawes (extension 224) Subject: [Histonet] New Histology and MOH's Technologist Job Opportunities To: Histonetters Message-ID: Content-Type: text/plain Hello Histonetters, My name is Matt Hawes and I am the Senior AP Recruiter here at Ategra Systems and I have received new Histology and MOH's Technologist Job Opportunities that I wanted to inform you about and see if you might be interested or if you might happen to know of anyone looking for new employment opportunities? Here are some of my HOTTEST Fulltime Permanent Histo Tech positions & MOH's Tech opportunities: 1. Boston Burbs, MA- MOH's/ Histo Tech- Day Shift 2. Rockville, MD- MOH's/Histo Tech- Day Shift 3. Northeastern MI- Histo Tech-Day Shift 4. Sioux City, IA- Histo Tech-Day Shift 5. Los Angeles, CA- IHC/Hist Supervisor 6. Rhode Island- Histo Tech-Day Shift 7. New Jersey-Histo Tech- Nite Shift (3 openings) 8. Upstate NY- Histo Tech- Day Shift (2 openings) These positions are as direct employees of our clients. They all offer full employee benefits (if working in a fulltime capacity): excellent healthcare, life insurance, dental, vision, 401K/retirement - as well as relocation bonuses (where applic). If you are interested in these jobs, please CALL ME ASAP at (800)466 9919 x224 or e-mail me at Matt@ategra.com . To speed things up, please also send me a copy of your resume, (if you haven't already done so). If you are interested in jobs outside the above-mentioned areas, please send me your resume as well as I have clients throughout the US. I will keep your resume confidential and will not release it to anyone without your permission (This is Ategra policy as well as my own). My services are at no charge to you. Of course, you may be happy in your present job, but it never hurts to to keep an eye open. Also, if you have friends/peers who might be interested as well, if you could pass my query & name on to them I'd be very grateful. However, if you are interested in any of the jobs above, please call me. Thank You !! Matt Hawes Senior Laboratory Recruiter Ategra Systems (800) 466-9919 Ext 234 Matt@Ategra.com --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing VOICE: 407-671-5800 ext 224 TOLLFREE: 800-466-9919 Ext 224 Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu To Learn More About Ategra: http://www.ategra.com -------------------------------------------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. -------------------------------------------------------------------------------------------------- ------------------------------ Message: 13 Date: Tue, 19 Apr 2005 08:41:41 -0500 From: "Steven Coakley" Subject: [Histonet] Qc forms To: Message-ID: Content-Type: text/plain; charset="us-ascii" I am starting up a histo lab and in need of varies qc forms. I got a few from Carsons. Any would be appreciated. Email me at sjchtascp@yahoo.com Thanks ------------------------------ Message: 14 Date: Tue, 19 Apr 2005 08:49:03 -0500 From: "Flores, Teresa" Subject: [Histonet] QC & QA's To: histonet@lists.utsouthwestern.edu Cc: histo@nsh.org Message-ID: Content-Type: text/plain I know I have seen them, but is there a site for Histotechnology QC and QA on sectioning techniques, staining techniques and quality, etc? Teresa Flores LSUHSC NO, LA ------------------------------ Message: 15 Date: Tue, 19 Apr 2005 10:56:21 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] neutral stains for protozoa To: "'Yaskovich, Ruth A \(NIH/NIDCR\)'" , "'Gary Radice'" , "'Jennifer MacDonald'" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: <200504191656.j3JGuCOD021472@chip.viawest.net> Content-Type: text/plain; charset="US-ASCII" I use a H&E counterstain for Von Kossa. I use gills 3 hematoxylin and do not destain in acid alcohol/ I do blue briefly in ammonia water, wash well and then use a 0.2% aqueous eosin for 30 min., dehydrate quickly thru 95% alcohols, 100 and xylene then permount. This method will stain osteoid on bone viewed by fluorescence UV. It is very nice for histomorphometry by image analysis as only the osteoid lights up and the rest of the tissue (calcified bone and blue nuclei) is dark under UV, makes it really easy to measure osteoid seams. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) Sent: Monday, April 18, 2005 12:28 PM To: Gary Radice; 'Jennifer MacDonald' Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] neutral stains for protozoa You can stain with the Von Kossa with a nuclear fast red counterstain. Ruth Yaskovich N.I.H. > ---------- > From: Jennifer MacDonald > Sent: Monday, April 18, 2005 2:18 PM > To: Gary Radice > Cc: Histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] neutral stains for protozoa > > There is an iron hematoxylin procedure for protozoa. It is in the > AFIP manual. I don't have it. It is Heidens or something similar. > > > > > > > Gary Radice > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/31/2005 06:47 AM > > To > Histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] neutral stains for protozoa > > > > > > > I have a colleague who is studying a calcified protozoan. She is > specifically interested in calcification, and she is looking for > stains she can use that are not acidic and that don't require acid > differentiation steps, so that she doesn't inadvertently decalcify the > specimens. > > Any thoughts about nuclear or counterstains that might work in this > situation? > > > > Gary P. Radice gradice@richmond.edu > Department of Biology 804-289-8107 (voice) > University of Richmond 804-289-8233 (FAX) > Richmond VA 23173 http://www.richmond.edu/~gradice > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 17, Issue 31 **************************************** From David.Edmondson <@t> christie-tr.nwest.nhs.uk Wed Apr 20 11:49:08 2005 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Fungus Control Message-ID: And I tried a block with another vegetable fungus (ratatouille leftovers) ) combined with a sliver of lung, so we could see when the elastic had come up. Dave Christie Hospital Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jason and Heather Sent: 19 April 2005 20:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fungus Control My lab is in need of fungus control. We are willing to trade AFB control for fungus. I guess we live in a fungus free enviroment in South Dakota. If anybody can help please let me know. Jason McGough HT Clinical Laboratory of the Black Hills 2805 5th St. Rapid City, SD 57701 605-343-2267 themagoos@rushmore.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Mueller <@t> kp.org Wed Apr 20 12:15:29 2005 From: David.Mueller <@t> kp.org (David.Mueller@kp.org) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] DAB Waste Disposal/Treatment Message-ID: Hi-- I work for the EH&S Department of Kaiser Permanente in San Francisco. We're looking for ways to reduce our DAB waste. Is anyone currently using DAB/Out or any other method of treatment? And if so, are you pleased with the system? Also--we've had trouble getting a firm answer from Triangle Biomedical as to what's contained in the remaining liquid once the DAB has been filtered out. Can anyone explain this further--we want to make sure it's something that can be poured down the drain in California. Thanks for your help. David Mueller Environmental Health and Safety Coordinator Kaiser Permanente San Francisco Medical Center From juan.gutierrez <@t> christushealth.org Wed Apr 20 12:47:24 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] slide dryer Message-ID: MOPEC has a nice one that is a little bit larger and spins the racks while drying. Thermo has a couple of new ones that I haven't tried yet. Good luck. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clarke, Mary Sent: Wednesday, April 20, 2005 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide dryer I have an old slide dryer from Lipshaw that is dying and wondering if anyone knows where I can get a replacement. It measures about 10x7x5 inches. The model number is 218. Thanks in advance for your help. Terri Clarke Histology Supervisor All Saints Laboratory 3801 Spring Street Racine, Wi 53405 mclarke@allsaintshealthcare.org 262-687-6609 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, April 19, 2005 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 17, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Methyl green pyronin staining, methyl green source (John A. Kiernan) 2. EDTA (Trisha Emry) 3. snout (Trisha Emry) 4. IHC fixation (Steven Coakley) 5. Re: EDTA (Gayle Callis) 6. Re: Microwave Processors (Robyn Vazquez) 7. RE: (no subject) (Jennifer MacDonald) 8. Re: neutral stains for protozoa (Jennifer MacDonald) 9. RE: neutral stains for protozoa (Yaskovich, Ruth A (NIH/NIDCR)) 10. Paraffin processing late stage mouse embryos (Johnson, Teri) 11. RE: Cryostat (Charles Scouten) 12. New Histology and MOH's Technologist Job Opportunities (Matt Hawes (extension 224)) 13. Qc forms (Steven Coakley) 14. QC & QA's (Flores, Teresa) 15. RE: neutral stains for protozoa (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Mon, 18 Apr 2005 13:15:31 -0400 From: "John A. Kiernan" Subject: Re: [Histonet] Methyl green pyronin staining, methyl green source To: Gayle Callis Cc: Histonet@lists.utsouthwestern.edu Message-ID: <4263EB33.7494C303@uwo.ca> Content-Type: text/plain; charset=us-ascii The dye sold by Sigma is not methyl green. It is ethyl green, which is better. They call it methyl green on the label, but with the correct CI number (the one for ethyl green). Ethyl green can be found also under its right name in the Sigma catalog, with a cross-reference to the methyl green entry (which has ethyl green as a synonym, and the correct molecular formula for ethyl green). I don't know why they go on calling their ethyl green methyl green. The latter is a dye nobody would want these days because of its inevitable contamination with crystal violet. The CV originates during manufacture of MG and is also produced by spontaneous degradation in the stored dye powder. Ethyl green is made in a different way and it is also much more stable than methyl green. If anyone sells real methyl green, it must be from very old stock because that dye has not been manufactured for many (?30-40) years. See Floyd Green: Sigma-Aldrich Handbook of Stains, Dyes and Indicators (1991) for explanation of why ethyl green in not contaminated and does not change slowly into crystal violet. The differences between the two dyes are explained also in both the 9th and the 10th editions of Conn's Biological Stains. The staining method for DNA & RNA has, in reality, been ethyl green-pyronine for many years. Recognition of this fact in staining manuals and catalogs is long overdue. A standardized EGP method (Hoyer et al 1986 Histochem. J. 18:90-94) is technically simpler than MGP procedures that date from the days of real methyl green. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Gayle Callis wrote: > > Following the thread here. > > One can purchase methyl violet free Methyl reen from Sigma. This avoids > the messy procedure to remove methyl violet using toxic chloroform and a > separating funnel inside a fume hood. We changed to Sigmas MG instead of > taking a chance other companies methyl green dye lots were not MV free. > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 18 Apr 2005 22:35:43 -0700 From: "Trisha Emry" Subject: [Histonet] EDTA To: "histo" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I can buy two kinds of EDTA through my local university stores. One can be used for electrophoresis (Fisher BP 120-500 $27.38) and the other just listed as powder (Fisher S311-500 $36.37). With the great price difference (we have been using the $36.37 type) I was wondering if someone could tell me if there really is a difference and if I have to continue to use the pricey type. Thanks, Trisha U of Washington, Seattle ------------------------------ Message: 3 Date: Mon, 18 Apr 2005 22:40:39 -0700 From: "Trisha Emry" Subject: [Histonet] snout To: "Histonet@lists. utsouthwestern. edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We are decalcifying large 4x4x1 or 2 inch pieces of pig snout and the limits of my microtome make it impossible for me to cut. Are there any contract labs out there that can do the sectioning when we get them decalcified? Thanks, Trisha U of Washingtion, Seattle ------------------------------ Message: 4 Date: Mon, 18 Apr 2005 13:23:30 -0500 From: "Steven Coakley" Subject: [Histonet] IHC fixation To: Message-ID: Content-Type: text/plain; charset="us-ascii" Inquiring about formalin substitutes for IHC in order to eliminate or decrease antigen retrieval. Steven ------------------------------ Message: 5 Date: Mon, 18 Apr 2005 12:33:13 -0600 From: Gayle Callis Subject: Re: [Histonet] EDTA To: "Trisha Emry" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050418120752.01b4b1b0@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed E sure to know which EDTA you are working with, there are several. EDTA disodium salt, it will work for decalcification and if only soluble at ~ 10 g/100 ml water with pH of 4 to 6 (5% solution) EDTA (no sodiums added) will work also. It is only soluble at ~10g/100 ml water, and will have pH of approx 4 to 6 (5% solution). EDTA tetrasodium salt is very soluble in buffers/water, as much as 14g/100 mls, but is very alkaline at pH 9, you must adjust the pH down and Webb Jee (very famous bone person) used acetic acid. This is the EDTA we prefer for bone work. Fisher catalog show no great differences between these two different grades of EDTA. (Fisher BP 120-500 $27.38) is electrophoresis grade, has been tested for lots of different things, found under Bioreagent listings and is the disodium salt. (Fisher S311-500 $36.37) is ACS certified found in analytical section of their catalog, extensively tested, and is the a disodium salt. It helps to keep a Fisher chemical catalog around to check out chemical purity, etc, etc. Either will work, go for the cheapest. If all you are doing is decalcification, then technical grade, even cheaper works just as well, the tetrasodium salt is technical grade. At 11:35 PM 4/18/2005, you wrote: >I can buy two kinds of EDTA through my local university stores. > >One can be used for electrophoresis (Fisher BP 120-500 $27.38) >and the other just listed as powder (Fisher S311-500 $36.37). > >With the great price difference (we have been using the $36.37 type) I was >wondering if someone could tell me if there really is a difference and if I >have to continue to use the pricey type. > >Thanks, >Trisha >U of Washington, Seattle > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 6 Date: Mon, 18 Apr 2005 11:34:46 -0700 From: "Robyn Vazquez" Subject: Re: [Histonet] Microwave Processors To: conniegrubaugh@hotmail.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Connie, Make sure it is a complete tissue processing microwave. We have a Ted Pella, which would be great except it doesn't have water loader and therefore it doesn't process properly. My department isn't willing to buy it. We are moving and will purchasing a tissue processor. Robyn OHSU ------------------------------ Message: 7 Date: Mon, 18 Apr 2005 11:37:30 -0700 From: Jennifer MacDonald Subject: RE: [Histonet] (no subject) To: "Kristen Broomall" Cc: 'Histonet' , 'Shawn Leslie' , histonet-bounces@lists.utsouthwestern.edu, 'Shirley Powell' , 'Gareth Davis' Message-ID: Content-Type: text/plain; charset="US-ASCII" Beginning in 2001, the ASCP added cytology preparation and staining to the HT exam. "Kristen Broomall" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/12/2005 02:20 PM To "'Shirley Powell'" , "'Gareth Davis'" , "'Shawn Leslie'" , "'Histonet'" cc Subject RE: [Histonet] (no subject) I can attest to that too. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shirley Powell Sent: Tuesday, April 12, 2005 5:16 PM To: 'Gareth Davis'; 'Shawn Leslie'; 'Histonet' Subject: RE: [Histonet] (no subject) A histotech I know took the HT last weekend here and told me there was a lot more cytology than expected on hers. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gareth Davis Sent: Tuesday, April 12, 2005 3:50 PM To: Shawn Leslie; Histonet Subject: Re: [Histonet] (no subject) Hi, I took the HT computer test last December. One helpful hint I can give is to know everything about tissue idenitification, what stain is needed for that tissue, what may go wrong with stain for a specific tissue and how to identify mistakes. I saw so many images, and it really caught me offguard. I expected many, but not as many as I saw and the questions to the images were not what I expected. I did pass with a good score, but I was really surprised that I did. Good luck. Gareth Davis --- Shawn Leslie wrote: > > > To All, > > I have a trainee who is about to sit for her HT computer exam, and > wondered if anyone has taken it recently, and could offer some helpful > hints. Thanks > > Shawn Leslie > > Confidentiality Notice: This e-mail message, including any > attachments, may contain confidential information. The information is > intended only for the use of the individual(s) or entity named above. > If you are not the intended recipient, you are notified that any > disclosure, copying, distribution, or the taking of any action in > reliance on the contents of this e-mail information is prohibited. > If you have received this e-mail in error, please contact the sender > by reply e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Yahoo! Small Business - Try our new resources site! http://smallbusiness.yahoo.com/resources/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 18 Apr 2005 12:18:08 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] neutral stains for protozoa To: Gary Radice Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" There is an iron hematoxylin procedure for protozoa. It is in the AFIP manual. I don't have it. It is Heidens or something similar. Gary Radice Sent by: histonet-bounces@lists.utsouthwestern.edu 03/31/2005 06:47 AM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] neutral stains for protozoa I have a colleague who is studying a calcified protozoan. She is specifically interested in calcification, and she is looking for stains she can use that are not acidic and that don't require acid differentiation steps, so that she doesn't inadvertently decalcify the specimens. Any thoughts about nuclear or counterstains that might work in this situation? Gary P. Radice gradice@richmond.edu Department of Biology 804-289-8107 (voice) University of Richmond 804-289-8233 (FAX) Richmond VA 23173 http://www.richmond.edu/~gradice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 18 Apr 2005 15:27:32 -0400 From: "Yaskovich, Ruth A (NIH/NIDCR)" Subject: RE: [Histonet] neutral stains for protozoa To: Gary Radice , 'Jennifer MacDonald' Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: <8F3AB322628548428A992EFB0E80F5D315D630D6@nihexchange8.nih.gov> Content-Type: text/plain; charset="iso-8859-1" You can stain with the Von Kossa with a nuclear fast red counterstain. Ruth Yaskovich N.I.H. > ---------- > From: Jennifer MacDonald > Sent: Monday, April 18, 2005 2:18 PM > To: Gary Radice > Cc: Histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] neutral stains for protozoa > > There is an iron hematoxylin procedure for protozoa. It is in the AFIP > manual. I don't have it. It is Heidens or something similar. > > > > > > > Gary Radice > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/31/2005 06:47 AM > > To > Histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] neutral stains for protozoa > > > > > > > I have a colleague who is studying a calcified protozoan. She is > specifically interested in calcification, and she is looking for stains > she can use that are not acidic and that don't require acid > differentiation steps, so that she doesn't inadvertently decalcify the > specimens. > > Any thoughts about nuclear or counterstains that might work in this > situation? > > > > Gary P. Radice gradice@richmond.edu > Department of Biology 804-289-8107 (voice) > University of Richmond 804-289-8233 (FAX) > Richmond VA 23173 http://www.richmond.edu/~gradice > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 10 Date: Mon, 18 Apr 2005 14:48:00 -0500 From: "Johnson, Teri" Subject: [Histonet] Paraffin processing late stage mouse embryos To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Is anybody doing paraffin processing on E18.5 - P0 mouse embryos that can help us optimize our in-house protocol? I know that when possible one should bisect embryos for best fixation and processing. Unfortunately we can't always do that. In some instances, we are able to bisect the samples just posterior to the forelimbs and process only the top 1/2 of the embryo. Even after poking some holes and immersion for 72 hours in 10% NBF, combined with a rigorous processing schedule (2 hours/station with a typical automated tissue processor setup--same schedule as we use for decalcified whole adult mouse heads!), our samples just aren't sectioning well, and they tend to blow up on the water bath. A test with samples without holes poked in them vs. ones with holes produced no discernable improvement in processing. A google search turned up one lab (evidently hand processing) who recommends removing the skin of this size embryo (E15.5 - E18.5) and removing the head and/or limbs if not needed for study. Fixation and processing: fix 1 hour @ 40 degrees C, change fixative, then 40 degrees C overnight. The following are done at room temp or refrigerated for IHC or ISH: dehydrating in 30% ETOH 1-2 hrs, 50% ETOH 1-2 hrs, 70% ETOH 4 hrs or O/N, 95% ETOH 3 hrs x 2 or O/N, and 100% ETOH 1 hr x 2. Embryos can be stored at -20 degrees C at this point. Carrying on with dehydration and infiltration: 100% ETOH 1 hr x 3 @ RT, Xylene 30-40 min x 2 (check embryos*), paraffin 40 minutes x 3, paraffin embedding. *No indication of what we're actually checking on these samples I do think that removing the skin on these guys would help immensely. I also think that additional dissection when possible is critical to good fixation and processing. Any comments on the above processing schedule? Is anybody doing these later stage mouse embryo samples using a protocol that's working for you? Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org ------------------------------ Message: 11 Date: Mon, 18 Apr 2005 15:13:49 -0500 From: "Charles Scouten" Subject: RE: [Histonet] Cryostat To: "Chung, Luong" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <5784D843593D874C93E9BADCB87342AB44F87C@tpiserver03.Coretech-holdings.com> Content-Type: text/plain; charset="iso-8859-1" See the following link: This works great. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=475102&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats&idsubcategory=182 Cordially, Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Monday, April 18, 2005 8:14 AM To: Histonet (E-mail) Subject: [Histonet] Cryostat All, I'm looking for a reliable cryostat with self disinfection. Any suggestion? Thanks Bruce Chung, MSM, HT/CT(ASCP) Anatomic Pathology Manager Phoebe Putney Memorial Hospital lchung@ppmh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 18 Apr 2005 17:03:51 -0400 From: Matt Hawes (extension 224) Subject: [Histonet] New Histology and MOH's Technologist Job Opportunities To: Histonetters Message-ID: Content-Type: text/plain Hello Histonetters, My name is Matt Hawes and I am the Senior AP Recruiter here at Ategra Systems and I have received new Histology and MOH's Technologist Job Opportunities that I wanted to inform you about and see if you might be interested or if you might happen to know of anyone looking for new employment opportunities? Here are some of my HOTTEST Fulltime Permanent Histo Tech positions & MOH's Tech opportunities: 1. Boston Burbs, MA- MOH's/ Histo Tech- Day Shift 2. Rockville, MD- MOH's/Histo Tech- Day Shift 3. Northeastern MI- Histo Tech-Day Shift 4. Sioux City, IA- Histo Tech-Day Shift 5. Los Angeles, CA- IHC/Hist Supervisor 6. Rhode Island- Histo Tech-Day Shift 7. New Jersey-Histo Tech- Nite Shift (3 openings) 8. Upstate NY- Histo Tech- Day Shift (2 openings) These positions are as direct employees of our clients. They all offer full employee benefits (if working in a fulltime capacity): excellent healthcare, life insurance, dental, vision, 401K/retirement - as well as relocation bonuses (where applic). If you are interested in these jobs, please CALL ME ASAP at (800)466 9919 x224 or e-mail me at Matt@ategra.com . To speed things up, please also send me a copy of your resume, (if you haven't already done so). If you are interested in jobs outside the above-mentioned areas, please send me your resume as well as I have clients throughout the US. I will keep your resume confidential and will not release it to anyone without your permission (This is Ategra policy as well as my own). My services are at no charge to you. Of course, you may be happy in your present job, but it never hurts to to keep an eye open. Also, if you have friends/peers who might be interested as well, if you could pass my query & name on to them I'd be very grateful. However, if you are interested in any of the jobs above, please call me. Thank You !! Matt Hawes Senior Laboratory Recruiter Ategra Systems (800) 466-9919 Ext 234 Matt@Ategra.com --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing VOICE: 407-671-5800 ext 224 TOLLFREE: 800-466-9919 Ext 224 Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu To Learn More About Ategra: http://www.ategra.com -------------------------------------------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. -------------------------------------------------------------------------------------------------- ------------------------------ Message: 13 Date: Tue, 19 Apr 2005 08:41:41 -0500 From: "Steven Coakley" Subject: [Histonet] Qc forms To: Message-ID: Content-Type: text/plain; charset="us-ascii" I am starting up a histo lab and in need of varies qc forms. I got a few from Carsons. Any would be appreciated. Email me at sjchtascp@yahoo.com Thanks ------------------------------ Message: 14 Date: Tue, 19 Apr 2005 08:49:03 -0500 From: "Flores, Teresa" Subject: [Histonet] QC & QA's To: histonet@lists.utsouthwestern.edu Cc: histo@nsh.org Message-ID: Content-Type: text/plain I know I have seen them, but is there a site for Histotechnology QC and QA on sectioning techniques, staining techniques and quality, etc? Teresa Flores LSUHSC NO, LA ------------------------------ Message: 15 Date: Tue, 19 Apr 2005 10:56:21 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] neutral stains for protozoa To: "'Yaskovich, Ruth A \(NIH/NIDCR\)'" , "'Gary Radice'" , "'Jennifer MacDonald'" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: <200504191656.j3JGuCOD021472@chip.viawest.net> Content-Type: text/plain; charset="US-ASCII" I use a H&E counterstain for Von Kossa. I use gills 3 hematoxylin and do not destain in acid alcohol/ I do blue briefly in ammonia water, wash well and then use a 0.2% aqueous eosin for 30 min., dehydrate quickly thru 95% alcohols, 100 and xylene then permount. This method will stain osteoid on bone viewed by fluorescence UV. It is very nice for histomorphometry by image analysis as only the osteoid lights up and the rest of the tissue (calcified bone and blue nuclei) is dark under UV, makes it really easy to measure osteoid seams. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) Sent: Monday, April 18, 2005 12:28 PM To: Gary Radice; 'Jennifer MacDonald' Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] neutral stains for protozoa You can stain with the Von Kossa with a nuclear fast red counterstain. Ruth Yaskovich N.I.H. > ---------- > From: Jennifer MacDonald > Sent: Monday, April 18, 2005 2:18 PM > To: Gary Radice > Cc: Histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] neutral stains for protozoa > > There is an iron hematoxylin procedure for protozoa. It is in the > AFIP manual. I don't have it. It is Heidens or something similar. > > > > > > > Gary Radice > Sent by: histonet-bounces@lists.utsouthwestern.edu > 03/31/2005 06:47 AM > > To > Histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] neutral stains for protozoa > > > > > > > I have a colleague who is studying a calcified protozoan. She is > specifically interested in calcification, and she is looking for > stains she can use that are not acidic and that don't require acid > differentiation steps, so that she doesn't inadvertently decalcify the > specimens. > > Any thoughts about nuclear or counterstains that might work in this > situation? > > > > Gary P. Radice gradice@richmond.edu > Department of Biology 804-289-8107 (voice) > University of Richmond 804-289-8233 (FAX) > Richmond VA 23173 http://www.richmond.edu/~gradice > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 17, Issue 31 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Apr 20 13:48:37 2005 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Stain for Proteoglycans References: <9d225e9d3506.9d35069d225e@wiscmail.wisc.edu> Message-ID: <4266A405.F4DF308F@uwo.ca> If you simply want to stain all proteoglycans, use alcian blue at pH 2.5. It will bind to the glycosaminoglycan components, which make up the bulk of proteoglycan molecules. Alcian blue will also stain heparin (mast cell granules), but they are unlikely cause confusion. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ NIDAL E MUVARAK wrote: > > Is there a stain we could do to assess proteoglycan content in the mouse > pulmonary arteries? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Wed Apr 20 15:02:32 2005 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Cleaning Acid Message-ID: Hello to all in histoland. I need help in locating a cleaning acid solution for cleaning glassware. We are having a problem with our GMS stain. I think it has something to do with the glassware. Thanks in advance Allison Scott CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From vazquezr <@t> ohsu.edu Wed Apr 20 16:14:42 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Cleaning Acid Message-ID: Allison, I use Micro 90 for glassware. I don't know if it would work with your glassware after doing a GMS. Robyn OHSU From jqb7 <@t> cdc.gov Wed Apr 20 16:37:56 2005 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Cleaning Acid Message-ID: We use 20% nitric acid in distilled water. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Scott, Allison D Sent: Wed 4/20/2005 4:02 PM To: 'histonet@lists.utsouthwestern.edu' Cc: Subject: [Histonet] Cleaning Acid Hello to all in histoland. I need help in locating a cleaning acid solution for cleaning glassware. We are having a problem with our GMS stain. I think it has something to do with the glassware. Thanks in advance Allison Scott CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 43 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Anni-Maija.Linden <@t> helsinki.fi Thu Apr 21 00:53:15 2005 From: Anni-Maija.Linden <@t> helsinki.fi (Anni-Maija Linden) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] rabbit anti-GFP (Molecular Probes) Message-ID: <1114062795.42673fcbcfd27@www3.helsinki.fi> Hi All, Do you have a simple method for GFP detection on mouse 4%PFA perfusion fixed free-floating brain sections using rabbit serum anti-GFP (Molecular Probes, A6455) and ABC-DAB detection. I would like to know how to block peroxidases effectively and which blocking buffer works best to get low background. Also optimal concentration of primary antibody? Thanks a lot. Best Regards, Anni-Maija -- Anni-Maija Linden, Ph.D. Institute of Biomedicine, Pharmacology University of Helsinki POB 63, 00014 University of Helsinki Finland tel. 358-9-191-25357 or 358-50-3441574 (gsm) email: anni-maija.linden@helsinki.fi From Andrew.MacDuff <@t> ed.ac.uk Thu Apr 21 02:43:04 2005 From: Andrew.MacDuff <@t> ed.ac.uk (Andrew MacDuff) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Mouse BAL Message-ID: <00bc01c54645$c04e1e80$b9b2d781@universi8lynpq> Dear All Does any one have a good method for bronchoalveolar lavage of mice and can you tell me what a normal number of recovered cells is? We tried yesterday and got very variable results- between 2000 to 185000 cells. Thanks for the help Andrew Andrew MacDuff Clinical Research Fellow Wilkie Laboratory Medical School Edinburgh University Teviot Place Edinburgh andrew.macduff@ed.ac.uk From leclercj <@t> vetanat.unizh.ch Thu Apr 21 03:22:08 2005 From: leclercj <@t> vetanat.unizh.ch (Leclerc Jocelyne) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] VDR Protein Message-ID: I need vitamin D receptor protein as a positive control. Where can i get it? Jocelyne Leclerc VetSuisse Fakult?t Veterin?r-Anatomisches Institut Winterthurerstr. 260 8057 Z?rich From juan.gutierrez <@t> christushealth.org Thu Apr 21 06:05:02 2005 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Stain for Proteoglycans Message-ID: Back in my biochemistry research days, we use to stain proteoglycans with coomassie blue for the protein core and alcian blue for the sulfated carbohydrate chains. This was done on gels, but you might try it on tissue. Good luck. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John A. Kiernan Sent: Wednesday, April 20, 2005 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Stain for Proteoglycans If you simply want to stain all proteoglycans, use alcian blue at pH 2.5. It will bind to the glycosaminoglycan components, which make up the bulk of proteoglycan molecules. Alcian blue will also stain heparin (mast cell granules), but they are unlikely cause confusion. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ NIDAL E MUVARAK wrote: > > Is there a stain we could do to assess proteoglycan content in the mouse > pulmonary arteries? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From padunnje <@t> iupui.edu Thu Apr 21 07:31:58 2005 From: padunnje <@t> iupui.edu (Dunn-Jena, Patsy A) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] Equipment requests Message-ID: I got hit by an unexpected request last pm so am asking for your help. 2 of the investigators asked about the following 3 items and I was not expecting anything like this with funds so short. I am a research lab and what I will be doing is primarily bone work, but I am doing some soft tissue work as well. Cryostat Dissecting microscope Microtome Vendors are welcome, but I am asking for your thoughts as to the good and bad. Patsy Patsy A. Dunn-Jena, RVT, LAT, HT (ASCP) Mineralized Tissue and Histology Research Laboratory Indiana University School of Dentistry 1121 W. Michigan Street, Room 238 Indianapolis, IN 46202 padunnje@iupui.edu (317) 274-0544 From ian.montgomery <@t> bio.gla.ac.uk Thu Apr 21 09:38:15 2005 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:24:59 2005 Subject: Fwd: [Histonet] Equipment requests Message-ID: <6.2.1.2.2.20050421152931.030238c0@udcf.gla.ac.uk> > >I got hit by an unexpected request last pm so am asking for your help. >2 of the investigators asked about the following 3 items and I was not >expecting anything like this with funds so short. I am a research lab >and what I will be doing is primarily bone work, but I am doing some >soft tissue work as well. > > > >Cryostat = I bought my Bright cryostat in 1974 and it's still in perfect >condition, need I say more. > >Dissecting microscope = Have a look at the latest Zeiss dissecting >microscopes, you'll be amazed. > >Microtome = Mmmm. I'm sure others will comment. I use Leica and my old >Leitz 1212 is still in daily use. > > > >Vendors are welcome, but I am asking for your thoughts as to the good >and bad. > > > >Patsy > > > >Patsy A. Dunn-Jena, RVT, LAT, HT (ASCP) > >Mineralized Tissue and Histology Research Laboratory > >Indiana University School of Dentistry > >1121 W. Michigan Street, Room 238 > >Indianapolis, IN 46202 > >padunnje@iupui.edu > >(317) 274-0544 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07623 975451 e-mail: ian.montgomery@bio.gla.ac.uk From pkarlisch <@t> psu.edu Thu Apr 21 09:43:20 2005 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Fri Sep 16 15:24:59 2005 Subject: [Histonet] All, Message-ID: All, We are interested in knowing how some of you handle the volume of Immunhistochemistries in your department if you are limited by staff and autostainers. Do you have cut off times for ordering; do you prioritize the work or do you have a turn around time that must be met and what is this time from ordering to finished slides. We have two autostainers that can only handle 20 slides each and 10-15% of the volume is performed manually. One tech of two rotates into this lab. I appreciate any help you can give me. Thank you, Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu From la.sebree <@t> hosp.wisc.edu Thu Apr 21 10:14:55 2005 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] All, Message-ID: Pat, We have a dedicated IHC/ISH lab with 2 full-time techs (an HT and an MT). We have 3 automated stainers capable of doing 70 slides. Our stated TAT is 24 hours which we most often meet or exceed. Biopsies in by 9am we attempt to have out the same day. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Thursday, April 21, 2005 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] All, All, We are interested in knowing how some of you handle the volume of Immunhistochemistries in your department if you are limited by staff and autostainers. Do you have cut off times for ordering; do you prioritize the work or do you have a turn around time that must be met and what is this time from ordering to finished slides. We have two autostainers that can only handle 20 slides each and 10-15% of the volume is performed manually. One tech of two rotates into this lab. I appreciate any help you can give me. Thank you, Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Thu Apr 21 10:32:20 2005 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Re: IHC TAT In-Reply-To: Message-ID: Hi Pat. One way we have maximized the use of stainers is to let them run overnight. We start a run in the late afternoon and let it run overnight. The slides are ready to be dehydrated & coverslipped when we come in the next morning. This seems to make efficient use of staff & instruments. We do have a cut off time. What we are going to start that night needs to be in the lab to be cut by noon (or so). We are lucky that we have a part time tech who cuts what we receive in the afternoon at night, and it?s ready to start in the morning. We can actually do 3 runs a day on our stainers by staggering start times of staff, starting a run early in the morning, a run mid-day if necessary, and the overnight run. If you can figure out your rate limiting steps, perhaps you can work around them. Patti Loykasek PhenoPath Laboratories Seattle, WA> All, > We are interested in knowing how some of you handle the volume of > Immunhistochemistries in your department if you are limited by staff and > autostainers. Do you have cut off times for ordering; do you prioritize > the work or do you have a turn around time that must be met and what is > this time from ordering to finished slides. > We have two autostainers that can only handle 20 slides each and > 10-15% of the volume is performed manually. One tech of two rotates > into this lab. > I appreciate any help you can give me. > Thank you, > Pat Karlisch > > Pat Karlisch > Supervisor, Histology, Pathology and Laboratory Medicine > Penn State Milton S. Hershey Medical Center > Mail Code H179 > Hershey, PA 17033 > Phone (717) 531-6072 > Fax: (717) 531- 7741 > email: pkarlisch@psu.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pzeitlow <@t> bbpllab.com Thu Apr 21 10:44:15 2005 From: pzeitlow <@t> bbpllab.com (Pat Zeitlow) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] All, Message-ID: <813FB33DA405334F947F8BFC6EBD0B2AE6A4@bbplsrv1.bbpl> We try to give a one day TAT from order to receipt of finished product. We set up the majority of our cases (usually all of our cases) by 9:30 a.m. Our pathologists receive the stained slides and are able to sign most cases out the same day they are stained. Any attempt to stain cases "real time", whether manual or automated, has resulted in a lot of $$ spent on running QC with each "run" and too much interference with the rest of our workflow. Our pathologists seemed please with the TAT. They can order IHCs any time on Tuesday (for instance) and have them back, interpreted, and the case reported by close of business Wednesday. Pat -----Original Message----- From: Patricia Karlisch [mailto:pkarlisch@psu.edu] Sent: Thursday, April 21, 2005 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] All, All, We are interested in knowing how some of you handle the volume of Immunhistochemistries in your department if you are limited by staff and autostainers. Do you have cut off times for ordering; do you prioritize the work or do you have a turn around time that must be met and what is this time from ordering to finished slides. We have two autostainers that can only handle 20 slides each and 10-15% of the volume is performed manually. One tech of two rotates into this lab. I appreciate any help you can give me. Thank you, Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathrm35 <@t> adelphia.net Thu Apr 21 12:55:03 2005 From: pathrm35 <@t> adelphia.net (pathrm35@adelphia.net) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] histotechnologist position in SE Florida Message-ID: <5614780.1114106103679.JavaMail.root@web3.mail.adelphia.net> Fellow tech's, We are currently seeking a FT histology technologists for our small but growing dermpath lab. We are seeking a HT or HTL (ASCP), Florida licensed technologist (not technician) or eligible with a minimum of an associates degree (in a science)and at least 5 years experience. Experience with immunos and grossing of skin specimens a plus. We offer: - a sign on bonus - a brand new 2,500 sf lab (we are currently moving in) - 2 great physicians to work for - close proximity to the ocean ( 10 minutes away) - no micro managment - competitive salary Ron Martin fax 561-721-1249 From joseph.tamasi <@t> bms.com Thu Apr 21 14:20:43 2005 From: joseph.tamasi <@t> bms.com (Joseph Tamasi) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] EGF Receptor Antibody Message-ID: <4267FD0B.7080303@bms.com> A colleague of mine is interested in demonstrationg EGF receptors in decalcified, paraffin embedded mouse and rat bone. Can anyone suggest a source for a good antibody against EGFR or her-1? Also, any procedure details (dilution, incubation times, etc) with these antibodies would be appreciated. Thanks in advance. Joe Tamasi Bristol-Myers Squibb Pennington, NJ From pathrm35 <@t> adelphia.net Thu Apr 21 15:45:18 2005 From: pathrm35 <@t> adelphia.net (pathrm35@adelphia.net) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] grossing of skin biopsies (again) Message-ID: <15351356.1114116318853.JavaMail.root@web8.mail.adelphia.net> I asked this question about 7 weeks ago but didn't get any clear definitive answers, so here I go again. Does anyone know what the requirements are for a tech to gross skin biopsies? We are a private dermpath lab in Florida. I need to know the educational and licensing requirements as I am seeking to fill a position. All help is greatly appreciated. Thanks, Ron Martin From histosci <@t> shentel.net Thu Apr 21 16:17:29 2005 From: histosci <@t> shentel.net (HSRL) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] PAS-Silver Stain Message-ID: <000001c546b7$8b4fcef0$0200a8c0@HSRLMAIN> Dear Netters, Does anyone have a protocol for a PAS-Silver stain combination? If so, it would be greatly appreciated if you would e-mail or fax me a copy or even point me in the right direction. Thanks a lot, Beth Poole HSRL, Inc.- A GLP Compliant Contract Laboratory 137 South Main Street Woodstock, Virginia 22664 (540)459-8211 Fax: (540)459-8217 beth@hsrl.org www.hsrl.org From conniemoss <@t> relia.net Thu Apr 21 16:21:19 2005 From: conniemoss <@t> relia.net (Connie McManus) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] woe is me In-Reply-To: <002b01c54597$6ad5ec70$7929f318@yourxhtr8hvc4p> References: <002b01c54597$6ad5ec70$7929f318@yourxhtr8hvc4p> Message-ID: <49339.208.186.240.165.1114118479.squirrel@email.relia.net> Louise, If you have access to a metalurgical saw with a diamond wheel, could you possibly cut them out individually, then re-embed each of these blocks of tissue in MMA in your regular mold?? I've done a similar thing with TEM samples before, but never with Histology-sized tissues. Just a thought. -- Connie McManus Mt Ogden Scientific Services 950 W Kershaw, Suite E Ogden UT 84401 tel: 801/334-6677 direct: 801/745-2583 cell: 435/757/2975 fax: 435/514-1781 conniemoss@relia.net www.mtogdensci.com > > Hi all, > > anyone know how I can rescue a whole bunch of samples that > polymerised into one huge mass in MMA? > > I have acess to all those great but toxic solvents such as chloroform, > acetone, toluene and xylene. (naturally I use these under appropriate > safety conditions, such as when smoking and eating my lunch ;o) ) > > thank you in advance > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "....I know who I am. No-one else knows who I am. If I was a giraffe, > and someone said I was a snake, I'd think no, actually I'm a giraffe" > Richard Gere > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Anti-Virus. > Version: 7.0.308 / Virus Database: 266.9.17 - Release Date: 4/19/2005 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ====== From conniemoss <@t> relia.net Thu Apr 21 16:29:09 2005 From: conniemoss <@t> relia.net (Connie McManus) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Website -- a small boast Message-ID: <49352.208.186.240.165.1114118949.squirrel@email.relia.net> Hi everyone! Our website is now up & running. My son, Nate, designed the whole thing. You have to understand, he had never done such a thing before. He learned to do this with some help from his friends. I'm just a tad proud. I Couldn't resist sending out this little boast. I invite you all to check it out in your spare time. http://www.mtogdensci.com While you're there, you might want to check out MOSS-LINK. This is a real-time viewing of what's in our scope, so you'll have to call our toll free number and ask for a demo. -- Connie McManus Mt Ogden Scientific Services 950 W Kershaw, Suite E Ogden UT 84401 toll free: 877/311-6677 tel: 801/334-6677 direct: 801/745-2583 cell: 435/757/2975 fax: 435/514-1781 conniemoss@relia.net www.mtogdensci.com ====== From Linresearch <@t> aol.com Thu Apr 21 16:38:19 2005 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] P450 Ab Message-ID: Hello, Can anyone recommend an P450 Ab for FFPE rodent tissues? Do you have a protocol? Lin From jkiernan <@t> uwo.ca Thu Apr 21 17:05:14 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] PAS-Silver Stain References: <000001c546b7$8b4fcef0$0200a8c0@HSRLMAIN> Message-ID: <4268239A.85AAA6A4@uwo.ca> What kind of silver stain? What do you want to see? ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 _______________________________ HSRL wrote: > > Dear Netters, > Does anyone have a protocol for a PAS-Silver stain combination? If so, > it would be greatly appreciated if you would e-mail or fax me a copy or > even point me in the right direction. > Thanks a lot, > > Beth Poole > > HSRL, Inc.- A GLP Compliant Contract Laboratory > > 137 South Main Street > > Woodstock, Virginia 22664 > > (540)459-8211 > > Fax: (540)459-8217 > > beth@hsrl.org > > www.hsrl.org From sayanarra <@t> yahoo.com Thu Apr 21 19:06:09 2005 From: sayanarra <@t> yahoo.com (saya narra) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] All, In-Reply-To: 6667 Message-ID: <20050422000610.98464.qmail@web60620.mail.yahoo.com> Linda, What type(s) of stainers do you have ? We have the VIP and autostainer which do the job. I don't know the best combination of automation / histotechs. I'd have all manual ops just to keep clear of the mouthy Australians ! Arrogance piled on attitude frosted with sarcasm topped with a dollop of condecension ! saya narra U of T "Sebree Linda A." wrote: Pat, We have a dedicated IHC/ISH lab with 2 full-time techs (an HT and an MT). We have 3 automated stainers capable of doing 70 slides. Our stated TAT is 24 hours which we most often meet or exceed. Biopsies in by 9am we attempt to have out the same day. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Thursday, April 21, 2005 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] All, All, We are interested in knowing how some of you handle the volume of Immunhistochemistries in your department if you are limited by staff and autostainers. Do you have cut off times for ordering; do you prioritize the work or do you have a turn around time that must be met and what is this time from ordering to finished slides. We have two autostainers that can only handle 20 slides each and 10-15% of the volume is performed manually. One tech of two rotates into this lab. I appreciate any help you can give me. Thank you, Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From sayanarra <@t> yahoo.com Thu Apr 21 19:28:09 2005 From: sayanarra <@t> yahoo.com (saya narra) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Processors In-Reply-To: 6667 Message-ID: <20050422002809.8032.qmail@web60621.mail.yahoo.com> Hello Sue, >From what I hear, Peloris TPs went into Ameripath and did nothing but breakdown, so the rumor goes. This may explain why they are being virtually given away to Ameripath. If you get one make sure 1) It's free to try - call US labs and see what their arrangement is - and 2) you make sure someone is close by to fix it. These suggestions are, of course, prudent for all instruments. Good luck . I prefer our VIPs fot TP. Faster isn't always better - tortoise and the hare !!! Saya Narra UofT Suelutsch@cs.com wrote: US Labs in Irvine California is awaiting their Peloris Processor. We were to receive two the beginning of April, but they say they are having software problems. We are really looking forward to trying this because of the amount of prostate we process. Has anyone yet tried this in Australia or anywhere else? __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Leslie.Chaussey <@t> uchospitals.edu Thu Apr 21 23:12:51 2005 From: Leslie.Chaussey <@t> uchospitals.edu (Leslie.Chaussey@uchospitals.edu) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Career opportunities Message-ID: <4CB46920276D5047BEBDA04E67BBF59F014F768D@uchadmb03.uchad.uchospitals.edu> We currently have the following positions open at the University of Chicago Hospitals Chief Technologist, Anatomic Pathology (supervisor) Sr Histotechnologist, Anatomic Pathology Histotechnologist, Anatomic Pathology Online applications can be completed at our web site www.uchospitals.edu Please feel free to respond to me directly if you have any questions. Leslie Chicago's a great city! ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Hospitals ******************************************************************************** From contact <@t> excaliburpathology.com Fri Apr 22 07:06:24 2005 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Kidney Tissue Message-ID: <20050422120624.68754.qmail@web50306.mail.yahoo.com> Hello, My lab is in need of normal and rejected transplant kidney tissue for IHC controls. I only need a small piece or two of each from the cortex containing glomeruli. Thanks, Paula Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From asmith <@t> mail.barry.edu Fri Apr 22 08:02:26 2005 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] woe is me Message-ID: <4C051EAE581BB646BF53A749A73FBA2D1F3CC0@exchsrv01.barrynet.barry.edu> A "Dremel" tool with an emery wheel would do the job. The flying powder is a bit irritating, so the job should be done in a fume hood. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connie McManus Sent: Thursday, April 21, 2005 5:21 PM To: Joe Nocito Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] woe is me Louise, If you have access to a metalurgical saw with a diamond wheel, could you possibly cut them out individually, then re-embed each of these blocks of tissue in MMA in your regular mold?? I've done a similar thing with TEM samples before, but never with Histology-sized tissues. Just a thought. -- Connie McManus Mt Ogden Scientific Services 950 W Kershaw, Suite E Ogden UT 84401 tel: 801/334-6677 direct: 801/745-2583 cell: 435/757/2975 fax: 435/514-1781 conniemoss@relia.net www.mtogdensci.com > > Hi all, > > anyone know how I can rescue a whole bunch of samples that > polymerised into one huge mass in MMA? > > I have acess to all those great but toxic solvents such as chloroform, > acetone, toluene and xylene. (naturally I use these under appropriate > safety conditions, such as when smoking and eating my lunch ;o) ) > > thank you in advance > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "....I know who I am. No-one else knows who I am. If I was a giraffe, > and someone said I was a snake, I'd think no, actually I'm a giraffe" > Richard Gere > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Anti-Virus. > Version: 7.0.308 / Virus Database: 266.9.17 - Release Date: 4/19/2005 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From Kemlo.Rogerson <@t> elht.nhs.uk Fri Apr 22 08:03:04 2005 From: Kemlo.Rogerson <@t> elht.nhs.uk (Kemlo Rogerson) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Kidney Tissue Message-ID: <1030B679AD69D6119C3F00080210DD9D05A3F203@bhrv-nt-11.bhrv.nwest.nhs.uk> I'll sell you a kidney, how much will you pay? 53 years old, one careful owner, washed regularly. Kemlo Rogerson Cellular Pathology Manager East Lancashire Hospitals NHS Trust DD. 01254-294162 Mobile 0774-9754194 -----Original Message----- From: Paula Pierce [mailto:contact@excaliburpathology.com] Sent: 22 April 2005 13:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Tissue Hello, My lab is in need of normal and rejected transplant kidney tissue for IHC controls. I only need a small piece or two of each from the cortex containing glomeruli. Thanks, Paula Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jhofecker <@t> yahoo.com Fri Apr 22 09:35:53 2005 From: jhofecker <@t> yahoo.com (Jennifer Hofecker) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] congo red on frozen sections Message-ID: <20050422143553.9020.qmail@web21006.mail.yahoo.com> Hi everyone, Happy Friday! Has anyone done a congo red on frozen sections? Which method do you use? Do you make any modifications to the procedure? I am attempting one on muscle frozen sections (my control is FFPE) and I am not sure what results to expect. Thank you all for you help. Jennifer Hofecker __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From celebrej <@t> HHSC.CA Fri Apr 22 10:59:38 2005 From: celebrej <@t> HHSC.CA (Celebre Julia) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] congo red on frozen sections Message-ID: <3AADFB88753AD31189C100902786B91C14E6DDE2@hch_nt_exchange.hhsc.ca> Happy Friday right back acha... We do Highman's Congo Red on frozen muscle biopsies that have been fixed in Baker's Formol Calcium for at least 10 minutes.. Usually works well.. Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext46145 email: celebrej@hhsc.ca -----Original Message----- From: Jennifer Hofecker [mailto:jhofecker@yahoo.com] Sent: Friday, April 22, 2005 10:36 AM To: histonet posts Subject: [Histonet] congo red on frozen sections Hi everyone, Happy Friday! Has anyone done a congo red on frozen sections? Which method do you use? Do you make any modifications to the procedure? I am attempting one on muscle frozen sections (my control is FFPE) and I am not sure what results to expect. Thank you all for you help. Jennifer Hofecker __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The Cornerstone of Care Campaign for Hamilton Health Sciences needs your support. Please, visit hamiltonhealthsciences.ca today and help make something great even greater. This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From lance.erickson <@t> ihc.com Fri Apr 22 11:04:02 2005 From: lance.erickson <@t> ihc.com (Lance Erickson) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] grossing of skin biopsies (again) Message-ID: Take a look at the CLIA website for federally regulated personnel qualification guidelines here: http://www.cms.hhs.gov/clia/apcsubm.pdf Basically it dictates that performing gross dictation in any form is considered high complexity testing. Specifics for the number of hours of classes is found there. ?493.1489 - On or before April 24 1995 (I) be a high school graduate or equivalent; and (b) have documentation of training appropriate for the test performed before analyzing patient specimens. After that date it requires an associate degree in a biological or chemical science or medical laboratory technology -or- qualify as a medical technologist with a bachelor's degree from an accredited institution -or- earned a bachelor's degree in a chemical, physical, biologic or clinical laboratory science. Interpretive Guidelines ?493.1489(b)(7)In the case of gross examinations, the technical supervisor may delegate to individuals qualified under ?493.1489 the responsibility for the physical examination/description, including color, weight, measurement and other characteristics of the tissue; or other mechanical procedures for which a specific written protocol has been developed. The technical supervisor is ultimately responsible for the diagnosis related to the gross examination and must sign the examination report. The technical supervisor is not required to provide direct onsite supervision but is responsible for the accuracy of all test results reported. All physical examinations/descriptions of tissue including color, weight, measurement and other characteristics of the tissue; or other mechanical procedures performed in the absence of the technical supervisor by individuals qualified under ?493.1489 should be reviewed within 24 hours by the technical supervisor. All microscopic tissue examinations must be performed by individuals qualified under ?493.1449(b), (l) or (m), as appropriate. This is for CLIA certified laboratories. The state of Florida may have more guidlines. Lance Erickson Anatomic Pathology Supervisor Primary Children's Medical Center Salt Lake City, UT >>> 04/21/05 2:45 PM >>> I asked this question about 7 weeks ago but didn't get any clear definitive answers, so here I go again. Does anyone know what the requirements are for a tech to gross skin biopsies? We are a private dermpath lab in Florida. I need to know the educational and licensing requirements as I am seeking to fill a position. All help is greatly appreciated. Thanks, Ron Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rudy.salcedo <@t> christushealth.org Fri Apr 22 12:25:18 2005 From: rudy.salcedo <@t> christushealth.org (Salcedo, Rudy) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Histology Tech, Position open Message-ID: Full-time histology Tech Position open at Christus Spohn Shoreline in Corpus Christi, Texas Must be HT with at least 5 years experience, rotate weekend (every 4th?) you can call and speak with Tammy Atwood @ 361-881-3920 or fax your resume to 361-885-0343. Thanks, Rudy Salcedo From jengirl1014 <@t> yahoo.com Fri Apr 22 12:32:38 2005 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Quick question---Maybe a long answer Message-ID: <20050422173238.96104.qmail@web60613.mail.yahoo.com> I have a PI that wishes to get cryosections from a biopsy he did. However, he freezed the tissue at -80C and then came to me with how to fix it, embed it, and section it. Have the following questions: Can this be done? What is the best way to do this? I've only done it from live tissue, not frozen. Please list any and all chemicals that you use and the place that you get them from. Thank you in advance and I appreciate every answer that I get. Jennifer K. Sipes, RALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 cell: 443-413-0853 e-mail: jengirl1014@yahoo.com __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From kerry.l.crabb <@t> gsk.com Fri Apr 22 12:38:47 2005 From: kerry.l.crabb <@t> gsk.com (kerry.l.crabb@gsk.com) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Kerry L Crabb/PharmRD/GSK is out of the office. Message-ID: I will be out of the office starting 22-Apr-2005 and will not return until 25-Apr-2005. Contact Eve about necropsy issues and contact Jim Nold about histology issues. I will respond to messages when I return. From michael.j.hurle <@t> gsk.com Fri Apr 22 12:47:45 2005 From: michael.j.hurle <@t> gsk.com (michael.j.hurle@gsk.com) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Michael J Hurle/PharmRD/GSK is out of the office. Message-ID: I will be out of the office starting 22-Apr-2005 and will not return until 05-May-2005. From eileen_dusek <@t> yahoo.com Fri Apr 22 14:26:32 2005 From: eileen_dusek <@t> yahoo.com (eileen dusek) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Pathos Processor Message-ID: <20050422192632.17578.qmail@web30513.mail.mud.yahoo.com> Hi Everyone, Does anyone have experience with the Pathos Processor from Milestone. I appreciate pros and cons. Eileen Dusek Edward Hospital __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From cmeiers <@t> thelabrat.com Sat Apr 23 08:56:53 2005 From: cmeiers <@t> thelabrat.com (Christophe Meiers) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] CD4 CD8 stain Message-ID: <20050423135653.6C2397260@sitemail.everyone.net> Could someone please give advice regarding cd4 and cd8 staining of brain tissue. We dissected out the brain of a mouse, made longitudinal section midbrain, one half of the brain was placed in formalin for routine H & E, the other half was snap frozen for the cd4 and cd8. The sections came out absolutely horrible with what looked like exploded tissue. Should the brain be treated in a different fashion ? Would formalin interfere with staining CD4 and CD8 ? I know this probably sounds like a very elementary question, we are currently waiting for a new Histo tech that is well versed in Immuno staining, but in the meantime research carries on. I am thanking you in advance and would appreciate any comments. As you have guessed, I am not a Histologist, just someone trying to keep the lab going until we fulfill the vacancy. Thank you Christophe Meiers _________________________________________________________________ Search the web here using Google-http://www.thelabrat.com/search.html From histo20 <@t> hotmail.com Sun Apr 24 16:58:40 2005 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Performing Immuno Stains Message-ID: Hello everyone! Does anyone know if a tech needs to be registered to perform immuno staining? Are immunos considered high complexity testing or not? We will be starting to do these in the very near future and I was just wondering about this. Thanks so much for your help - Paula Wilder St. Joseph Medical Center From DOOLEEO <@t> shands.ufl.edu Mon Apr 25 08:32:43 2005 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Parathyroid Hormone Message-ID: Dr. Histonetters, Vendors, Does anyone have an anti-parathyroid hormone antibody that works well on formalin fixed paraffin embedded tissue. The company I was using discontinued it. Thanks in advance Elaine Dooley HTL Shands Teaching Hospital Gainesville Florida 352-265-0111 ext 7-2117 From eca9 <@t> georgetown.edu Mon Apr 25 08:59:34 2005 From: eca9 <@t> georgetown.edu (Eva C Anderson) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] need some help with frozen sections Message-ID: <426CF7C6.3070002@georgetown.edu> Good morning, I am starting to stain frozen sections for the first time. Until now I have only stained paraffin embedded sections and so was hoping for some pointers. I contacted our histopathology lab who provided me with the following information: They don't use any antigen retrieval method. They pretreat the slides only with methanol for 10min followed by drying before staining. Then they proceed with the normal steps of staining and dehydration. I have however read that acetone can be used instead of the methanol. What is the difference that this provides? I am trying to stain for Stat5. Is there anything els I should keep in mind when handling frozen sections? Would be very grateful for any information you could provide this rookie with. Thank you, Eva From settembr <@t> umdnj.edu Mon Apr 25 10:18:54 2005 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Parathyroid Hormone Message-ID: Hello Elaine, I use parathyroid hormone from NovoCastra which is now distributed by Vision BioSystems in Norwell, Massachuetts I use it on human tissue at a dilution of 1:800. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Elaine Dooley 4/25/2005 9:32:43 AM >>> Dr. Histonetters, Vendors, Does anyone have an anti-parathyroid hormone antibody that works well on formalin fixed paraffin embedded tissue. The company I was using discontinued it. Thanks in advance Elaine Dooley HTL Shands Teaching Hospital Gainesville Florida 352-265-0111 ext 7-2117 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbass <@t> bidmc.harvard.edu Mon Apr 25 12:21:41 2005 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] GFP again Message-ID: <971ab99ff4f9d5a18b6bcaf122da48b6@bidmc.harvard.edu> Hey Guys, I have been searching the internet and all sorts of forums, including histonet trying to find the answers to my GFP questions. Essentially, I have a viral vector that I would like to inject into the liver which expresses EGFP. I would like to have the easiest way to determine if it works or not. I have heard that it is virtually impossible to detect native fluorescence with GFP, and that frozen cryostat sections completely lose the signal. Am I missing something here? There is a paper that is essentially doing the same thing that I would like to do, and they have enough signal that they can see it in the intact animal with a UV lamp, or in 6 micron cryosections without fixation or perfusion. According to everything I read, this shouldn't work. Does EGFP produce a stronger signal that can be seen in this way? The paper is: Nature Biotechnol 2005 Mar; 23(3):321-8. Any suggestions on how to process the liver such that I can see the native EGFP signal would be greatly appreciated. I have access to a cryostat and a microtome. I can freeze the tissue, perfuse the mouse, whatever it takes. I would prefer not to use immunostaining initially, as I have to section through entire lobes to visualize where the virus diffuses, etc. I can use an antibody once I get a rough estimate of where the signal is, but we don't have enough money to immunostain every section. Thanks, Caroline Bass From steven.coakley <@t> mirusbio.com Thu Apr 21 12:52:48 2005 From: steven.coakley <@t> mirusbio.com (Steven Coakley) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] paraffins Message-ID: Does anyone have any experience with surgpath IF200 infiltration medium and EM400 embedding medium. I'm considering using 2 different paraffins instead of one of the duel use paraffins. Is infiltation really improved by using the "softer" paraffin. Any opinions recommended. I'm starting up a new histo lab. Thanks, Steven From cfavara <@t> niaid.nih.gov Mon Apr 25 12:55:57 2005 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] GFP again Message-ID: Caroline, I am pretty much a novice at the GFP but have done some work on this. I can tell you that the protein is very soluble and does not survive standard fixation with NBF when working with frozen sections. There is a nice paper J Histochem Cytochem. 2003 Mar;51(3):401-4 that discusses this and proposes a vapor fixation. We have had good luck with formalin fixed paraffin embedded tissue using anti GFP antibodies. In my hands there is considerable variation in the sensitivity and signal /noise ratio. My suspicion is that due to the solubility of the protein perfused specimens may be preferable. But I have not tested this. We have started a project with grafting of GFP expressing tissue into a non GFP expressing mouse and I can tell you that seeing the GFP in the fresh tissue is not as obvious as some of the literature would lead one to believe. I am still working on this so I look forward to replies from others more knowledgeable and experienced than I. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Caroline Bass [mailto:cbass@bidmc.harvard.edu] Sent: Monday, April 25, 2005 10:22 AM To: Subject: [Histonet] GFP again Hey Guys, I have been searching the internet and all sorts of forums, including histonet trying to find the answers to my GFP questions. Essentially, I have a viral vector that I would like to inject into the liver which expresses EGFP. I would like to have the easiest way to determine if it works or not. I have heard that it is virtually impossible to detect native fluorescence with GFP, and that frozen cryostat sections completely lose the signal. Am I missing something here? There is a paper that is essentially doing the same thing that I would like to do, and they have enough signal that they can see it in the intact animal with a UV lamp, or in 6 micron cryosections without fixation or perfusion. According to everything I read, this shouldn't work. Does EGFP produce a stronger signal that can be seen in this way? The paper is: Nature Biotechnol 2005 Mar; 23(3):321-8. Any suggestions on how to process the liver such that I can see the native EGFP signal would be greatly appreciated. I have access to a cryostat and a microtome. I can freeze the tissue, perfuse the mouse, whatever it takes. I would prefer not to use immunostaining initially, as I have to section through entire lobes to visualize where the virus diffuses, etc. I can use an antibody once I get a rough estimate of where the signal is, but we don't have enough money to immunostain every section. Thanks, Caroline Bass _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pex0220 <@t> yahoo.com.cn Mon Apr 25 12:58:05 2005 From: pex0220 <@t> yahoo.com.cn (=?gb2312?q?=CC=EC=20=D0=C1?=) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Immunofluorescence about cartilage Message-ID: <20050425175805.63358.qmail@web15506.mail.cnb.yahoo.com> Hello,all, I am doing double immunofluorescence in bone sections. One protein was mainly expressed in cytoplasm, but we found that some positive expressions appeared among chondrocytes, moreover their expressions were stronger, my teacher told me, it could be due to this condition that part cartilages were broken during the staining so that this protein came out from chondrocytes, then positive expressions were present among chondrocytes. If it is true, what should I do to avoid this situation? If you have some experience about it, please do me a favor! Thank you! In addition, which methods can be used to reduce background? Guofeng Qian --------------------------------- Do You Yahoo!? ×¢²áÊÀ½çÒ»Á÷Æ·ÖʵÄÑÅ»¢Ãâ·ÑµçÓÊ From gcallis <@t> montana.edu Mon Apr 25 13:16:02 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] reply to murine CD4 and CD8 in brain sections Message-ID: <6.0.0.22.1.20050425120037.01b0cba8@gemini.msu.montana.edu> Christophe Meiers wrote: Could someone please give advice regarding cd4 and cd8 staining of brain tissue. We dissected out the brain of a mouse, made longitudinal section midbrain, one half of the brain was placed in formalin for routine H & E, the other half was snap frozen for the cd4 and cd8. The sections came out absolutely horrible with what looked like exploded tissue. Should the brain be treated in a different fashion ? Would formalin interfere with staining CD4 and CD8 ? *****You will have to frozen sections on fresh, unfixed snap frozen brain. Brain should be embedded in OCT, then it must MUST be snap frozen at very cold temps with either a dry ice/2 methylbutane mixture or another adequate method. Snap freezing has been discussed many times on Histonet, for methods do a search of Histonet Archives at www.histosearch.org. Formalin totally kills murine CD4 and CD8 antigen, and no retrieval or enzyme digestion will ever recover the antigens. Make sure block temperature and knife temperature are the same, equilibrate block (if stored at -80C) 20 min or so before cutting. Too cold a block means crunchy sections. Cryosection brain at -16C, 5 um sections, pick up on Plus Charge slides, air dry overnight, fix in 75%acetone/25% absolute ethanol (no substitute on alcohol can be used) for 5 min at room temperature, go directly to a buffer rinse FROM the fixative, 3 changes of buffer, and proceed with staining. Do not store your frozen sections in cryostat after cutting, go directly to air drying. We put our sections over a 16 mesh silica gel to ensure dryness. Some people use acetone fixation at 4C for 10 min, but the acetone/alcohol improves morphology and keeps excellent immunostaining. Peroxidase block, DAKO S2001 for 10 min Normal serum block for 30 min (10% goat/2.5%mouse in TBS or Dulbeccos PBS with 0.05% Tween 20. We add 0.2% goat serum to rinse buffers along with 0.05% Tween 20 Avidin/biotin block kit Vector CD4 Rat antimouse BD Pharmigen 1:500 (0.5mg/ml conc) in 5% goat serum. CD8 Rat antimouse 1:200 (0.5mg/ml conc) in 5% goat serum IgG isotype matched controls for these antibodies at same concentration in ug/ml is negative control Incubate 30 min, RT Goat antiRat F(ab')2 frag of IgG (0.5mg.ml) diluted 1:250 in normal serum block (yes! with mouse serum) for 30 min Strepavidin-HRP 1:500 from Biosource/TAGO diluted in rinse buffer, 20 min AEC+ DAKO control color development of positive control (normal spleen works) for approx 3 min Rinse, counterstain, and mount with aqueous mounting media. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From jkiernan <@t> uwo.ca Mon Apr 25 13:33:16 2005 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] paraffins References: Message-ID: <426D37EC.6949BC0E@uwo.ca> The subject of different paraffins was discussed at some length on Histonet more than 5 years ago. The most authoritative comments seemed to be from Russ Allison. His principal conclusion was that ingredients other than the hydrocarbons (the wax itself) made no difference to cutting properties. Russ has published in this field; the following list is probably incomplete. Allison RT (1978) The crystaline nature of histology waxes: a preliminary communication. Medical Laboratory Sciences 35: 355-363. Allison RT (1979) The crystaline nature of histology waxes: the effects of microtomy on the micro-structure of paraffin wax in sections. Medical Laboratory Sciences 36: 359-372. Allison RT, Lloyd D (1996) Measuring infiltration during paraffin wax processing for histology. British Journal of Biomedical Science 53: 235-237. Allison RT, Bryant D (1998) Effects of processing at 45C on staining. Biotechnic & Histochemistry 73: 128-136. Allison RT (2002) Tissue processing and sectioning. Chapter 7 in Microscopy and Histology for Molecular Biologists (Kiernan JA & Mason I eds). London: Portland Press. pp 144-169. In the most recent of these publications, Russ concedes that there may be some circumstances where addition of DMSO or a synthetic polymer could be advantageous. Unfortunately the archives at www.histosearch.com do not include the older correspondence about waxes. Russ Allison does not seem to be around on Histonet these days; a pity! -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Steven Coakley wrote: > > Does anyone have any experience with surgpath IF200 infiltration medium and > EM400 embedding medium. I'm considering using 2 different paraffins instead > of one of the duel use paraffins. Is infiltation really improved by using > the "softer" paraffin. Any opinions recommended. I'm starting up a new > histo lab. > > Thanks, > > Steven > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Apr 25 13:42:39 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] GFP, the ongoing dilemma with DsRed included Message-ID: <6.0.0.22.1.20050425121642.01b40a58@gemini.msu.montana.edu> Dear Caroline, You wrote: I have been searching the internet and all sorts of forums, including histonet trying to find the answers to my GFP questions. Essentially, I have a viral vector that I would like to inject into the liver which expresses EGFP. I would like to have the easiest way to determine if it works or not. I have heard that it is virtually impossible to detect native fluorescence with GFP, and that frozen cryostat sections completely lose the signal. Am I missing something here? There is a paper that is essentially doing the same thing that I would like to do, and they have enough signal that they can see it in the intact animal with a UV lamp, or in 6 micron cryosections without fixation or perfusion. According to everything I read, this shouldn't work. Does EGFP produce a stronger signal that can be seen in this way? The paper is: Nature Biotechnol 2005 Mar; 23(3):321-8. Any suggestions on how to process the liver such that I can see the native EGFP signal would be greatly appreciated. I have access to a cryostat and a microtome. I can freeze the tissue, perfuse the mouse, whatever it takes. I would prefer not to use immunostaining initially, as I have to section through entire lobes to visualize where the virus diffuses, etc. I can use an antibody once I get a rough estimate of where the signal is, but we don't have enough money to immunostain every section. ***We recently undertook a similar project with undecalcified murine nasal turbinate and small intestine tissue sections from mouse. In order to do immunofluorescence staining for a CD marker or dendritic cell, we were required to fix with acetone or acetone/alcohol, which immediately killed the eGFP and it failed to glow. This was also going be a problem with DsRED, a bioluminescent protein from Coral rather than jellyfish. One major problem is increasing autofluorescence in tissues by using formalin or paraformaldehyde fixatives. We could never get the best of both worlds with eGFP or DsRed. Final solution to the problem: With DsRed, unfixed fresh snap frozen tissues were cryosectioned at 5 um, mounted on Erie Plus Gold slides (a plus charged surface for extra holding power),and air dried for 30 min or more in front of a fan at RT. Air dried sections were rinsed one slide at time to prevent unfixed section loss with 2 changes PBS gentle agitation. We wanted to get rid of OCT. A coverslip was mounted with Prolong Gold antifade mounting media, ready to use containing DAPI for DNA/RNA. Results: spectacular DsRed where it was supposed to be, with nuclei counterfluorescing a lovely blue from DAPI staining DNA/RNA. The mounting media is a hard set. The only autofluorescence is natural, but does NOT interfere with the brighter signal of DsRed, and would not interfere with eGFP either. We are going to do this with eGFP next week to have green fluorescence with the DAPI stained nuclei. I have a review of autofluorescence I am going to attach to you. For more on eGFP and DsRed, go to Clontech website and download their Living Colours Manuals for these proteins. When you get to immunostaining, say for for a CD marker along with eGFP, we did our acetone/alcohol fixation, but detected eGFP with Goat antiGFP from Rockland (a superb antibody that is NOT expensive!) and came back with Donkey antiGoat-FITC, along with a biotinylated CD marker antibody and Strepavidin Alexa 555. This worked well for double fluorescence with the CD marker. As for DsRed, the antiDsRed antibody is pricey, so doing IFA staining for GFP has been more cost effective. From gcallis <@t> montana.edu Mon Apr 25 14:02:41 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] First time with frozen sections, long reply Message-ID: <6.0.0.22.1.20050425124550.01b4c780@gemini.msu.montana.edu> You wrote: I am starting to stain frozen sections for the first time. Until now I have only stained paraffin embedded sections and so was hoping for some pointers.I contacted our histopathology lab who provided me with the following information: They don't use any antigen retrieval method. They pretreat the slides only with methanol for 10min followed by drying before staining. Then they proceed with the normal steps of staining and dehydration. I have however read that acetone can be used instead of the methanol. What is the difference that this provides? I am trying to stain for Stat5. Is there anything els I should keep in mind when handling frozen sections? Questions: human tissue, animal tissue? Is the tissue fresh, unfixed or prefixed prior to snap freezing? Have you snap frozen (needed to minimize freezing artifact from water ice crystal formation) the tissues rather than cryostat freezing (too warm)? What antigens do you want to stain for? Fixation is dependent on the antigen, some will be fine with acetone, some may work better with another fixative. Antigen retrieval on frozen sections of fresh unfixed tissues is not needed as long a formalin or even paraformaldehyde is avoided. In general, AR tends to damage FS. If you plan to do CD marker staining, methanol can cause poor staining of these due to methanolic bridge formation. Avoid methanol as a fixative or in peroxidase block, use DAKO S2002 peroxidase block designed for gentle endog perox blocking, it doesn't contain MEOH and wil not chew section off the slide. Hydrogen peroxide can be put into PBS instead. Antibodies also need to be optimized since FS permit lower conc of antibodies. Dilution panels should be done. What does the spec sheet or manufacturer recommend for fixation of FS for your STAT5 antibody? You can always call their tech services and ask. Be gentle, FS are more fragile, and air dry them AFTER sectioning, DO NOT STORE newly cut setions in a cryostat. Avoid water condensation and freeze thawing of sections - this can damage antigens. Air drying overnight in front of a fan, or over dessicant is a good way to insure properly dried sections. Check Histonet Archives www.histosearch.org, it has tons of info on HOW to do frozen sections, fixation, blocking, and staining. Chris van der Loos has provided many excellent replies on frozen section IHC. DakoCytomation website has Immunochemical Staining Methods Handbook, 3rd Edition with tons of hints on what you want to do. Good luck Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From pkarlisch <@t> psu.edu Mon Apr 25 16:49:21 2005 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Surgical Volume vs. number of tests Message-ID: Histonetters: First, thank you all for the wonderful responses that you sent regarding the immunohistochemical staining relative to the volume of stains done per day. Your responses were very helpful. I would like to pose another question to you. Do you think there is over utilization with ordering immunohistochemistries on a daily basis? Relative to your surgical cases what would you say that your percentage (or actual numbers) of immunohistochemical cases are and how many slides would that equate to. For example: 6-8% of all our cases require immunohistochemistry, thus as volume increases so do our immuno stains. We do on average 1400 immuno slides/month which includes surgical, biopsy, bone marrow, Cytology FNA's and skins with one tech on rotation and two stainers with a capacity of 40 slides total. (Our yearly surgicals are about 34000 cases). We do very little research in this lab and minimal duplicate staining for file, study or education. Do any of you employ a method to discourage over utilization of immuno staining? Thank you again for any advice you can give me. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu From Janet.Bonner <@t> FLHOSP.ORG Mon Apr 25 17:18:45 2005 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Surgical Volume vs. number of tests Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB424D@fh2k093.fhmis.net> The workload/slides seems in line with ours. The only discouraging factor to ordering certain immunos is the ability to charge the patient/insurance company AND to justify the order/charges if called on the carpet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patricia Karlisch Sent: Monday, April 25, 2005 5:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Surgical Volume vs. number of tests Histonetters: First, thank you all for the wonderful responses that you sent regarding the immunohistochemical staining relative to the volume of stains done per day. Your responses were very helpful. I would like to pose another question to you. Do you think there is over utilization with ordering immunohistochemistries on a daily basis? Relative to your surgical cases what would you say that your percentage (or actual numbers) of immunohistochemical cases are and how many slides would that equate to. For example: 6-8% of all our cases require immunohistochemistry, thus as volume increases so do our immuno stains. We do on average 1400 immuno slides/month which includes surgical, biopsy, bone marrow, Cytology FNA's and skins with one tech on rotation and two stainers with a capacity of 40 slides total. (Our yearly surgicals are about 34000 cases). We do very little research in this lab and minimal duplicate staining for file, study or education. Do any of you employ a method to discourage over utilization of immuno staining? Thank you again for any advice you can give me. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Mon Apr 25 20:24:33 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Surgical Volume vs. number of tests Message-ID: <20050426012433.21115.qmail@web30410.mail.mud.yahoo.com> Dear Pat, I will offer a pathologists point of view. The actual percent cases which will need immuno will vary depending on several factors. First the case mix. You are in an academic center. I would expect you would have a larger percentage of demanding cases than a small private hospital. For example if you did only hernias, gallbladders and seb kers, you would not need any immunos. If you are doing all lymphomas and and soft tissue tumors you would have a %100 immuno rate. Experience and degree of specialization of the pathologists will play a role. In many situations the less experienced people tend to order a bit more than the more experienced people because of the lack of confidence that comes with experience. This is a natural evolution of the life of the pathologist much as your experience has tought you to find the simplest path. More academic pathologists with subspecialties may study certain cases in more depth because of their fields of interest. Also being an academic center with residents pathologists will often order more complete panals even though the diagnosis is clear for the benifit of the residents experience. Taking those facts out of the equation, I would be surprised if everyone reading this has not experienced increasing percentages of immuno requests over the past years. The number of antibodies required to carry out what would be considered " standard of practice" by our friends in the legal profession has grown enormously. And they will continue to grow. They are extremely helpful and represent one of the most significant advances in our profession. But have no fear. I predict the day is coming when your percentage of immunos will start to creep down only to be replaced by more specific, more intellectually demanding, and probably more expensive molecular methods! As far as trying to discourage over utilization, that is a budget vs. medical care issue and is under the perview of your medical director. It is his job to optimize care while keeping to the budget. You would have to be an experienced surgical pathologist to even begin make this judgement. One thing you could do to see if there is a particular culprit is to audit the ordering patterns of the different pathologists. Your computer system may be able to give you this easily.If you believe somebody is being wasteful with ordering of studies it is your job to bring it up with your superiors. When it works its way up, it can be looked at by a someone who can evaluate the situation from both sides. If there is an undo amout of tests being ordered this could possibly be reduced by more intradepartmental consultation. Sometimes sharing cases before immunos are ordered can prevent over ordering. It can also backfire and end up getting you more requests! When I show one of my cases to colleagues invariably I end up ordering a few more stains to satisfy their curiosity. If the hospital is pressuring you from the budget side then take a good hard look at where you can save without jeopardizing patient care. I would rather them take away the heat and the toilet paper than force me take guesses by cutting back on necessary studies. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From dpconsult <@t> earthlink.net Tue Apr 26 02:24:19 2005 From: dpconsult <@t> earthlink.net (Dick Paulson [Source Medical Products]) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] NSH Self Assessment and Study Guide CD Message-ID: For those of you who ordered the CD but couldn't get it to work.. Good news. NSH sent me a CD to test. This is my response. The CD works on all the systems I tested. I tested on the following operating systems: Windows 98 Windows 2000 Windows XP Home Edition Windows XP Professional Edition All the operating systems have the most up to date Service Packs and updates applied. I tested with the disk in the CD drive and also created a folder and copied the contents of the CD to a folder I created on disk. I also created a folder on a Windows 2000 server with the CD contents and ran the program from a mapped drive to the server. I went through 2 Studies and 1 Test to completion. Everything works. This is a great learning tool. I hope this helps. The CD was sent on the 19th but I just got it today (25th). If you have any questions, please do not hesitate to contact me directly. Thank you, Source Medical Products Dick Paulson dpconsult@earthlink.net Phone (800) 393-6345 From je22r <@t> udcf.gla.ac.uk Tue Apr 26 03:50:05 2005 From: je22r <@t> udcf.gla.ac.uk (Julia Edgar) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] coating slides for paraffin sections of CNS tissue Message-ID: <000901c54a3c$f17e3300$cdead182@vet.gla.ac.uk> Dear All We've been using APES coated slides for collecting paraffin embedded CNS tissue for immunohistochemistry (with microwave treatment) but the sections are becoming unstuck, to some extent, during processing. I will be grateful if you can tell me what you are coating slides with for small pieces of CNS tissue. Thank you Julia Julia Edgar BSc (Hons), PhD University of Glasgow Tel: 0141 330 5818 e-mail: je22r@udcf.gla.ac.uk From c.m.vanderloos <@t> amc.uva.nl Tue Apr 26 04:01:54 2005 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] RE: need some help with frozen sections Message-ID: <42f77b432f60.432f6042f77b@amc.uva.nl> Eva, Some DO's and DON'T's with immunostaining of cryostat tissue sections: * After cutting, let the sections dry overnight at room temperature under a ventilator * Fix with cold acetone (10 min, 4C). Some people apply a "double acetone fixation" method: 2x 10 min acetone fixation with air-drying in between. This may improve the tissue morphology. * The quality of the acetone should be P.A. grade and don't use it twice. Re-distilled acetone cannot be used for fixation of cryo's. Traces of water in the acetone ruins the tissue morphology. * Be aware that acetone is not a real fixative like NBF. Acetone just solves the fatty membranes and coagulate the proteins. Cryostat tissue sections remain quite vulnerable with respect to surfactants like Triton-X100, Tween-20. These should be avoided. Be aware that in some autostainer washbuffers surfactants may be included. * To solve this problem of tissue vulnerability an extra fixation (after acetone-fixation and air-drying) with Zamboni's (1 min, RT) is optional. This extra fixation step also improves the tissue morphology. However, depending on the antigen to be IHC stained, this step may also decrease the IHC staining intensity. * The application of methanol, either as a fixative or as base for endogenous peroxidase activity blocking is (at least to my vision) absolutely excluded. For example, most human CD markers are completely destroyed by methanol. On the other hand I am aware of investigators who are using successfully an acetone/methanol mixture for fixation of mouse cryo's for staining CD4, CD8 etc. * Acetone is not a good fixative when staining nuclear antigens. This fixation will lead to quite fuzzy stained nuclei. Instead, a NBF-fixation (5 min, RT) works out well. Although NBF-fixation is used: do NOT apply heat-induced antigen retrieval. * Some antigens associated with fatty structures (for example, oxLDL, or apoB) will solve into acetone. In this case NBF (5 min) can be tested. Again, NO antigen retrieval! * Endogenous peroxidase activity can be blocked with 0.1% Na-azide + 0.3% peroxide in PBS or TBS (20 min, RT). This kills at least the endogenous peroxidase activity in erythrocytes effectively, but in neutrophils some will remain. If endogenous peroxidase activity of neutrophils is a real problem, glucose oxidase blocking method is optional (see Histonet archives). * At the end of the IHC staining procedure, after the chromogen step wash your slides with tap water. NEVER use distilled water as this will ruin the tissue section completely!!!! * Unlike with FFPE sections, aqueous solutions (depending on the chromogen used of course) is no problem for mounting cryostat tissue sections. * The concept of first cutting a tissue section and than fixation means "post-fixation". This is something different from a FFPE section that is first fixed as a block, than embedded and cut ("pre-fixation"). This means that soluble antigens may leak away from a "post-fixed" tissue section. Be aware of this when IHC staining any small protein (<50KD), cytokine, chemokine, hormone, etc. I hope this isn't too much frightening you. Happy staining! Chris van der Loos, PhD Dept. of Pathology Academical Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 fax: +31 20 6960389 e-mail: [1]c.m.vanderloos@amc.uva.nl ----- Original Message ----- From Eva C Anderson Date Mon, 25 Apr 2005 09:59:34 -0400 To histonet@lists.utsouthwestern.edu Subject [Histonet] need some help with frozen sections Good morning, I am starting to stain frozen sections for the first time. Until now I have only stained paraffin embedded sections and so was hoping for some pointers. I contacted our histopathology lab who provided me with the following information: They don't use any antigen retrieval method. They pretreat the slides only with methanol for 10min followed by drying before staining. Then they proceed with the normal steps of staining and dehydration. I have however read that acetone can be used instead of the methanol. What is the difference that this provides? I am trying to stain for Stat5. Is there anything els I should keep in mind when handling frozen sections? Would be very grateful for any information you could provide this rookie with. Thank you, Eva References 1. mailto:c.m.vanderloos@amc.uva.nl From GAshton <@t> PICR.man.ac.uk Tue Apr 26 05:14:18 2005 From: GAshton <@t> PICR.man.ac.uk (Garry Ashton) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] immuno-LCM Message-ID: Dear all, I'm currently trying to re-optimize an immuno technique on frozen sections that allows for the production of quality RNA after laser capture. Presently I have found that not allowing the sections to dry, storing at -80, and using a much shortened immuno method (2 mins max in all ab incubations), plus a shortened colour reaction, with an RNase inhibitor added at the ab and colour steps gives me reasonable results. Does anybody else in histoland use this technique regularly, and if so have they found results similar to me. Many thanks. Garry PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the Christie Hospital NHS Trust. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From cfavara <@t> niaid.nih.gov Tue Apr 26 08:05:54 2005 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] coating slides for paraffin sections of CNS tissue Message-ID: Julia, I work with primarily rodent CNS tissue and use Superfrost/Plus I get them from Fischer in the US Cat# 22-034-979 and very rarely have any tissue loss generally when I neglect to properly dry the slide. Hope this is helpful. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Julia Edgar [mailto:je22r@udcf.gla.ac.uk] Sent: Tuesday, April 26, 2005 1:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coating slides for paraffin sections of CNS tissue Dear All We've been using APES coated slides for collecting paraffin embedded CNS tissue for immunohistochemistry (with microwave treatment) but the sections are becoming unstuck, to some extent, during processing. I will be grateful if you can tell me what you are coating slides with for small pieces of CNS tissue. Thank you Julia Julia Edgar BSc (Hons), PhD University of Glasgow Tel: 0141 330 5818 e-mail: je22r@udcf.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernaweston <@t> hotmail.com Tue Apr 26 08:17:11 2005 From: bernaweston <@t> hotmail.com (Bernadette Weston) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] viability of placenta Message-ID: How long can a placenta be refrigerated and still be viable for testing as well as Histology? Bernadette Weston HT Histology Supervisor Barberton Citizens Hospital Barberton, OH From GDawson <@t> dynacaremilwaukee.com Tue Apr 26 08:16:38 2005 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Temp/Humidity Records? Message-ID: Just got back from vacation but I feel the need to comment on this. Although I haven't heard of this one, it would not surprise me. Documentation, documentation, documentation; it is the only thing between us and utter chaos...give me a break. Each day, my staff and I do our IHC runs that require heat. My instructions are very straight forward; unless the thermometer reads at least 97 degrees, the run cannot continue. If the bath isn't up to temp, the slides wait until it is. We check and check and check this same thermometer many times a day and the heat runs don't continue until the magic number is reached. Still, every day, we must lug the temperature log out and put in that entry. Maybe this is a bad example but I don't see how writing an entry in a temperature log for a reading we all know by heart is keeping the walls of this lab from crumbling down. At some point, doesn't the additional, meaningless documentations added onto the CAP inspection roster become just that, meaningless. I picture a person somewhere in a dark room racking his or her brain 24/7 for more "required" documentation for CAP inspections because every year I think that CAP couldn't possibly come up with more things to document, they do. I'm rambling, long story short, I'm sick of all this documentation. My Opinion, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From Dorothy.L.Webb <@t> HealthPartners.Com Tue Apr 26 09:18:08 2005 From: Dorothy.L.Webb <@t> HealthPartners.Com (Dorothy.L.Webb@HealthPartners.Com) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] position Message-ID: We have a full time opening for a histotechnician, day shift, and on call every 7th Saturday between the hours of 0800 and 1430. This position rotates into all aspects of histology and will be trained to be a "back-up" in our IHC area. We are a large city trauma 1 hospital in St. Paul, MN. with 12 pathologists on board. A nice place to work, so, come on board!! You can contact me by Email if interested or go on line to regions.com for an application site and full job description! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From BDUE <@t> PARTNERS.ORG Tue Apr 26 09:49:03 2005 From: BDUE <@t> PARTNERS.ORG (Due, Brice) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] coating slides for paraffin sections of CNS tissue Message-ID: <59C772E2D8EDF345AE1601F5B60B3CB50279CA@PHSXMB7.partners.org> Hello Julia, I work in a clinical neuropathology lab. We do impox, silver stains, etc. on both charged (superfrost) and uncharged slides. We also do giant sctions up to 4in. x 6 in. on plain (uncharged) glass slides. We rarely have adhesion problems. You must make absolutely sure the sections are fully dried before you melt and deparaffinize them. If you melt the paraffin too soon, a water film will become trapped and the sections will begin lifting in xylene. The best way is to leave the slides in a 37C oven overnight. Second best is room temp overnight. In my experience, the second most important factor in adhesion is section flatness. Wrinkles will not only fill up with water, but they will also not make contact with the slide. In some autopsy cases we also use STA-ON (chromated gelatin) adhesive, either in the water bath, or smeared on the slide, but the drying rules above are still mandatory. If overnight is too long for you, first try it, and then try cutting back the drying times until your problems reappear. Good luck, -brice Neuropathology Lab Brigham & Women's Hospital Boston -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Favara, Cynthia (NIH/NIAID) Sent: Tuesday, April 26, 2005 9:06 AM To: 'Julia Edgar'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] coating slides for paraffin sections of CNS tissue Julia, I work with primarily rodent CNS tissue and use Superfrost/Plus I get them from Fischer in the US Cat# 22-034-979 and very rarely have any tissue loss generally when I neglect to properly dry the slide. Hope this is helpful. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Julia Edgar [mailto:je22r@udcf.gla.ac.uk] Sent: Tuesday, April 26, 2005 1:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coating slides for paraffin sections of CNS tissue Dear All We've been using APES coated slides for collecting paraffin embedded CNS tissue for immunohistochemistry (with microwave treatment) but the sections are becoming unstuck, to some extent, during processing. I will be grateful if you can tell me what you are coating slides with for small pieces of CNS tissue. Thank you Julia Julia Edgar BSc (Hons), PhD University of Glasgow Tel: 0141 330 5818 e-mail: je22r@udcf.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DonnaWillis <@t> texashealth.org Tue Apr 26 09:55:20 2005 From: DonnaWillis <@t> texashealth.org (Willis, Donna) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Temp/Humidity Records? Message-ID: <4483B6EE0FA82A4EAED2B1E161E5E41147C7BD@ftwex02.txhealth.org> Glen, Just wait until the CLSI (formally NCCLS) Microwave Device Use in the Histolog Laboratory Guideline is added to CAP. Donna Willis Histology Lab Manager Harris Methodist Fort Worth, Tx -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, April 26, 2005 8:17 AM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? Just got back from vacation but I feel the need to comment on this. Although I haven't heard of this one, it would not surprise me. Documentation, documentation, documentation; it is the only thing between us and utter chaos...give me a break. Each day, my staff and I do our IHC runs that require heat. My instructions are very straight forward; unless the thermometer reads at least 97 degrees, the run cannot continue. If the bath isn't up to temp, the slides wait until it is. We check and check and check this same thermometer many times a day and the heat runs don't continue until the magic number is reached. Still, every day, we must lug the temperature log out and put in that entry. Maybe this is a bad example but I don't see how writing an entry in a temperature log for a reading we all know by heart is keeping the walls of this lab from crumbling down. At some point, doesn't the additional, meaningless documentations added onto the CAP inspection roster become just that, meaningless. I picture a person somewhere in a dark room racking his or her brain 24/7 for more "required" documentation for CAP inspections because every year I think that CAP couldn't possibly come up with more things to document, they do. I'm rambling, long story short, I'm sick of all this documentation. My Opinion, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From BlazekL <@t> childrensdayton.org Tue Apr 26 10:32:28 2005 From: BlazekL <@t> childrensdayton.org (Linda Blazek) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] temp/humidity Message-ID: Where in the CAP guidelines does it say that humidity must be documented? Linda Linda Blazek, HT (ASCP) Department of Pathology Children's Medical Center Dayton, Ohio 45404 (937) 641-3358 fax (937)641-5482 blazekl@childrensdayton.org From laurie.colbert <@t> huntingtonhospital.com Tue Apr 26 11:02:22 2005 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Lyme Disease Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628001C5C2DC@EXCHANGE1.huntingtonhospital.com> Will a warthin starry diagnose lyme disease? Is there an IHC stain that will stain for this? Laurie Colbert From jkiernan <@t> uwo.ca Tue Apr 26 11:28:58 2005 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Cleaning Acid (Long) References: Message-ID: <426E6C4A.D7D3D0A0@uwo.ca> An answer to a question asked last week about cleaning glassware for a silver staining method. Skip to the bottom line if you don't want to know the reasons. Glassware that has been used for silver methods can collect traces of metallic silver - sometimes enough to see as a mirror or grey marks. If the glassware is used again for silver staining, these traces serve as nuclei for deposition of more silver, and they are bigger than the desired nucleation sites in the sections. The staining method may simply fail or the chemical reaction may go wild, with nonspecific deposition of silver all over the place. Silver is soluble in nitric acid. My cleaning technique is to put a little concentrated HNO3 in the vessel (Coplin jar or larger tank) and carefully move it over all the inside surface, over a sink with running water so that any spilled drops of acid are quickly diluted. Visible silver (look in the corners) disappears instantly, so smaller amounts must also be removed. Pour the used nitric acid into a beaker containing tap water (for later neutralization and disposal). If the tap water becomes opalescent you have removed a significant amount of silver from the glass. Next - and this is important - Fill the vessel with PURE (eg distilled) water and empty it; do this twice so that the concentration of silver ions in the water adhering to the sides is infinitessimal. Tap water must not be used for these washes because it always contains anions (chloride, bicarbonate, others?) that form insoluble silver salts. Any colloidal silver chloride particles that stay on the glass will be partly reduced to silver by exposure to light and can be expected to provide nucleation sites in later silver staining methods. Finally dry the glassware by letting it drain and store the vessels upside-down in a closed cupboard. Deposited silver is not the only kind of dirt that can spoil silver staining. Any kind of organic chemical deposit (such as a fragment of a section) or even residue from evaporated tap water will work in the same way. Concentrated nitric acid quickly oxidizes and dissolves pretty well everything, with one notable exception. The exception is metallic gold. This can replace deposited silver in glassware used for gold-toning, a procedure often used to improve contrast in silvered preparations. Gold on glass may appear only as a light purple discoloration. Any colour that resists nitric acid is probably gold. It can be removed with aqua regia, which is a 3:1 mixture of concentrated nitric:hydrochloric acids. Make and use aqua regia in a fume hood because it emits fumes of chlorine and nitrogen oxides. I have resorted to aqua regia 3 or 4 times (in >30 years) to get rid of gold on glass. The obvious way to prevent contamination is to reserve certain jars and dishes for gold-toning and nothing else. This is not very practical if we do many different methods and do not have a cupboard big enough for all jars that might carry catalytic contaminants. Some people use Farmer's reducer (a solution containing potassium ferricyanide and sodium thiosulphate). This is an altogether gentler liquid than nitric acid and it can dissolve silver from black & white photographs. The action of Farmer's reducer on visibly discoloured glass is very slow, and this mixture is not going to destroy insoluble organic forms of dirt such as bits of tissue. Bottom line: Nitric acid, followed by pure (not tap) water. John Kiernan London, Canada. ________________________________ "Scott, Allison D" wrote: > > Hello to all in histoland. I need help in locating a cleaning acid solution > for cleaning glassware. We are having a problem with our GMS stain. I > think it has something to do with the glassware. Thanks in advance > Allison Scott ------------------------- From krat18 <@t> aol.com Tue Apr 26 11:45:33 2005 From: krat18 <@t> aol.com (krat18@aol.com) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Heinz Body Stain Message-ID: <8C718967FC22B1B-F98-C0F1@FWM-D40.sysops.aol.com> Our Hematology dept. asked us to develop a procedure for Heinz Body Stain. Does anyone have such a procedure that they could send us? Thanks for your help! Karen_Raterman@ssmhc.com From Rcartun <@t> harthosp.org Tue Apr 26 11:46:36 2005 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Lyme Disease Message-ID: I have IHC for Borrelia burgdorferi, but I have never seen a positive human case. I have obtained positive staining in animal tissue and, obviously, our positive control tissue (lung injected with B. burgdorferi). Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Laurie Colbert" 04/26/05 12:02PM >>> Will a warthin starry diagnose lyme disease? Is there an IHC stain that will stain for this? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------- Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From contact <@t> excaliburpathology.com Tue Apr 26 12:04:19 2005 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Heinz Body Stain Message-ID: <20050426170419.90234.qmail@web50310.mail.yahoo.com> Pour ketchup on it. Sorry, couldn't resist. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From BDUE <@t> PARTNERS.ORG Tue Apr 26 12:09:30 2005 From: BDUE <@t> PARTNERS.ORG (Due, Brice) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Cleaning Acid (Long)... Aqua Regia Correction? Message-ID: <59C772E2D8EDF345AE1601F5B60B3CB50279CF@PHSXMB7.partners.org> Hello John, as always, thank you for sharing your knowledge. below you list aqua regia as 3:1 nitric:hydrochloric. I have more often seen it as 1:3 nitric:hydrochloric, and this is what I've used before. Was this a typo or can it go both ways? Thanks, -brice Neuropathology Lab Brigham & Women's Hospital, Boston -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of John A. Kiernan Sent: Tuesday, April 26, 2005 12:29 PM To: Scott, Allison D Cc: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Cleaning Acid (Long) An answer to a question asked last week about cleaning glassware for a silver staining method. Skip to the bottom line if you don't want to know the reasons. Glassware that has been used for silver methods can collect traces of metallic silver - sometimes enough to see as a mirror or grey marks. If the glassware is used again for silver staining, these traces serve as nuclei for deposition of more silver, and they are bigger than the desired nucleation sites in the sections. The staining method may simply fail or the chemical reaction may go wild, with nonspecific deposition of silver all over the place. Silver is soluble in nitric acid. My cleaning technique is to put a little concentrated HNO3 in the vessel (Coplin jar or larger tank) and carefully move it over all the inside surface, over a sink with running water so that any spilled drops of acid are quickly diluted. Visible silver (look in the corners) disappears instantly, so smaller amounts must also be removed. Pour the used nitric acid into a beaker containing tap water (for later neutralization and disposal). If the tap water becomes opalescent you have removed a significant amount of silver from the glass. Next - and this is important - Fill the vessel with PURE (eg distilled) water and empty it; do this twice so that the concentration of silver ions in the water adhering to the sides is infinitessimal. Tap water must not be used for these washes because it always contains anions (chloride, bicarbonate, others?) that form insoluble silver salts. Any colloidal silver chloride particles that stay on the glass will be partly reduced to silver by exposure to light and can be expected to provide nucleation sites in later silver staining methods. Finally dry the glassware by letting it drain and store the vessels upside-down in a closed cupboard. Deposited silver is not the only kind of dirt that can spoil silver staining. Any kind of organic chemical deposit (such as a fragment of a section) or even residue from evaporated tap water will work in the same way. Concentrated nitric acid quickly oxidizes and dissolves pretty well everything, with one notable exception. The exception is metallic gold. This can replace deposited silver in glassware used for gold-toning, a procedure often used to improve contrast in silvered preparations. Gold on glass may appear only as a light purple discoloration. Any colour that resists nitric acid is probably gold. It can be removed with aqua regia, which is a 3:1 mixture of concentrated nitric:hydrochloric acids. Make and use aqua regia in a fume hood because it emits fumes of chlorine and nitrogen oxides. I have resorted to aqua regia 3 or 4 times (in >30 years) to get rid of gold on glass. The obvious way to prevent contamination is to reserve certain jars and dishes for gold-toning and nothing else. This is not very practical if we do many different methods and do not have a cupboard big enough for all jars that might carry catalytic contaminants. Some people use Farmer's reducer (a solution containing potassium ferricyanide and sodium thiosulphate). This is an altogether gentler liquid than nitric acid and it can dissolve silver from black & white photographs. The action of Farmer's reducer on visibly discoloured glass is very slow, and this mixture is not going to destroy insoluble organic forms of dirt such as bits of tissue. Bottom line: Nitric acid, followed by pure (not tap) water. John Kiernan London, Canada. ________________________________ "Scott, Allison D" wrote: > > Hello to all in histoland. I need help in locating a cleaning acid solution > for cleaning glassware. We are having a problem with our GMS stain. I > think it has something to do with the glassware. Thanks in advance > Allison Scott ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Tue Apr 26 12:11:27 2005 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] RE: need some help with frozen sections In-Reply-To: <42f77b432f60.432f6042f77b@amc.uva.nl> References: <42f77b432f60.432f6042f77b@amc.uva.nl> Message-ID: Dear Chris, This is an *excellent* summary of what to do and not to do for frozen section IHC! One thing though, you said: "At the end of the IHC staining procedure, after the chromogen step wash your slides with tap water. NEVER use distilled water as this will ruin the tissue section completely!!!!". Why do you think this is so? In my experience, I never use tap water after staining - only MilliQ ddH20 - and have no deleterious effects on my frozen sections of varying thicknesses of both mouse and human tissue - both fixed with NBF and acetone. Best regards, Andrea At 11:01 AM +0200 4/26/05, C.M. van der Loos wrote: > Eva, > > Some DO's and DON'T's with immunostaining of cryostat tissue sections: > * After cutting, let the sections dry overnight at room temperature > under a ventilator > * Fix with cold acetone (10 min, 4C). Some people apply a "double acetone fixation" method: 2x 10 min acetone fixation with ......... -- From gcallis <@t> montana.edu Tue Apr 26 12:45:19 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] CAVEAT on Methanol for acetone/alcohol fixation for murine CD IHC, RE: need help on frozen sections, In-Reply-To: <42f77b432f60.432f6042f77b@amc.uva.nl> References: <42f77b432f60.432f6042f77b@amc.uva.nl> Message-ID: <6.0.0.22.1.20050426093430.01b8ce00@gemini.msu.montana.edu> Chris's comments are also true for MURINE CD marker staining. Consequently, we avoid methanol for both fixation and peroxidase blocking on murine frozen sections for CD (lymphocyte) IHC staining. ******Caveat: We NEVER use methanol as a frozen section fixative for MURINE lymphocyte (CD) markers. The acetone/alcohol (AA) fixative we use with great success is a mixture of acetone and 100% ETHYL ALCOHOL, and NEVER Methanol. Overnight air dried frozen sections are fixed in: 75 mls ACS reagent grade acetone and 25 ml 100% ethanol for 5 minutes at RT. Sections are NEVER allowed to dry after this fixation but go directly to buffer, 3 changes. It obviously has worked well for us as we have one CD4 protocol where rat antiMouse CD4 monoclonal is diluted 1:15,000 (fading out at 1:20,000) of a 0.5 mg/ml concentration. #####Please note: This combination "AA " fixative is used for MURINE CD marker staining for frozen sections and NOT human CD markers. I defer to Chris's experience on human CD marker staining, as the ethanol component in AA will probably cause failure with human CD marker also. We are talking two very different species here. Chris wrote: > Some DO's and DON'T's with immunostaining of cryostat tissue sections: > * The application of methanol, either as a fixative or as > base for endogenous peroxidase activity blocking is > (at least to my vision) absolutely excluded. For example, most > human CD markers are completely destroyed by methanol.On > the other hand I am aware of investigators who are using > successfully an acetone/methanol mixture for fixation of > mouse cryo's for staining CD4, CD8 etc. I have talked about methanolic bridges in the past which may cause loss of CD marker staining, but maybe this is NOT the exact mechanism for MEOH damage to antigens. Jules Elias indicated in his book that it was "thought that immunoreactivity of intracellular antigens may be affected by MEOH destructive effects on surface membrane antigens." He never said what the action was of MEOH on surface markers, but this came to me by way of discussion with a knowledgable person. Biogenex, in some printed material, also indicated MEOH should be avoided for CD marker work. It would be interesting to pursue what MEOH actually does to CD surface marker???? Any takers on the chemistry of what is going on here?? Elias supported his statements with the following references from his book: Elias JM Immunohistopathology, a practical approach to diagnosis. First Edition ASCP press, p46 - 48. 1. McMillan et al. J Cutan Pathol 8:228, 1981. Demonstration in situ of T cells and T cell susets in lichen planus using monoclonal antibodies. 2. Matsumoto Y. simultaneous inhibition of endogenous avidin binding activity and peroxidase applicable for eht avidin biotin system using monoclonal antibodies. Histochemistry 83:325,1985 3. Van Duijin P. Histochemistry of DOPA factores: III. Inactivation experiments on the DOPA factores in neutrophilic and eosinophilic leucocytes and erythrocytes. Acta Physio. Pharmacol 5:428:1987. Recommendations from the experts (Chris van der Loos) are well taken in order to save time, reagents, and avoid frustrations. For certain murine CD IHC staining on frozen sections, we discovered that acetone fixation recommendations from the antibody manufacturers were often far less successful than using the acetone/ethanol mixture, a method graciously given to us from an murine IHC expert in the field. Now we do a fixative panel to determine what works best for any given antigen. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From ROrr <@t> enh.org Tue Apr 26 13:02:15 2005 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] "Probe On " slides. Message-ID: I have a case of Fisher brand "Probe On" slides and one of the "Probe On" slide holders/carriers. Is any one interested? I'll be glad to send to a good home. Thank-you, Becky Orr, CLA HT (ASCP) IHC Lead Evanston Northwestern Helathcare ph: 847-570-2771 From gcallis <@t> montana.edu Tue Apr 26 13:48:04 2005 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Distilled water rinse versus tap water rinsing, a curious thing In-Reply-To: References: <42f77b432f60.432f6042f77b@amc.uva.nl> Message-ID: <6.0.0.22.1.20050426122435.01b86e40@gemini.msu.montana.edu> Andrea and Chris, I have seen Chris's results on rinsing. We collaborated on this after I got home from visiting his lab. I repeated his rinsing experiments in my lab to see if I had the same problems. I did NOT get the same results he observed. Could this be caused by differences in another countries water and purification systems? Very curious why this happens as rinsing with distilled water is a very common practice. My experience with acetone or acetone/ethyl alcohol combo fixed mouse frozen section IHC is the same as Andrea's, no problems or damage to sections after either rinsing with MilliQ, RO or tap water. Acetone fixed sections are less robust than the AA combo fixed sections, but distilled water rinse was not a problem anyway. We do not use NBF for murine IHC work here. 11:11 AM 4/26/2005, you wrote: >Dear Chris, > >This is an *excellent* summary of what to do and not to do for frozen >section IHC! > >One thing though, you said: "At the end of the IHC staining procedure, >after the chromogen step wash your slides with tap water. NEVER use >distilled water as this will ruin the tissue section completely!!!!". Why >do you think this is so? In my experience, I never use tap water after >staining - only MilliQ ddH20 - and have no deleterious effects on my >frozen sections of varying thicknesses of both mouse and human tissue - >both fixed with NBF and acetone. > >Best regards, >Andrea > > >At 11:01 AM +0200 4/26/05, C.M. van der Loos wrote: >> Eva, >> >> Some DO's and DON'T's with immunostaining of cryostat tissue sections: >> * After cutting, let the sections dry overnight at room temperature >> under a ventilator >> * Fix with cold acetone (10 min, 4C). Some people apply a "double > acetone fixation" method: 2x 10 min acetone fixation with > ......... >- Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From azdudley <@t> hotmail.com Tue Apr 26 13:48:51 2005 From: azdudley <@t> hotmail.com (anita dudley) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] decontamination of cryostat Message-ID: I know this has been discussed before but the cap number ANP.24250 says the cryostat should be defrosted weekly, if used daily. is everyone doing this?? we clean ours with 70% ethanol weekly but we don't take it all down. thanks for the help. anita dudley, providence hosp. mobile, alabama From histo007 <@t> hotmail.com Tue Apr 26 13:57:18 2005 From: histo007 <@t> hotmail.com (Jim Ball) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] New Yor Society Meeting Message-ID: Could some one reply directlly to me concerning the New York Society meeting that is taking place the first part of May. I can only attend on Saturday, so This is the only day I will need information on. My e-mail address is histo007@hotmail.com and the name is James L. Ball From dellav <@t> musc.edu Tue Apr 26 14:35:50 2005 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] New Yor Society Meeting Message-ID: Greetings Jim Judy LaDuc is the president of the New York Histotechnological Society. she can be reached at jaladuc@capital.net for anyone else who may have similar needs, the state society presidents are listed on the NSH homepage www.nsh.org Vinnie >>> "Jim Ball" 04/26/05 02:57PM >>> Could some one reply directlly to me concerning the New York Society meeting that is taking place the first part of May. I can only attend on Saturday, so This is the only day I will need information on. My e-mail address is histo007@hotmail.com and the name is James L. Ball _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Tue Apr 26 14:57:21 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] decontamination of cryostat Message-ID: Hello, I bring it to room temp, drain it and wipe it out then I use Santimaster IV which will kill all. Then I use absolute to finish it and get rid of any residual water. I do this about every other week. I clean it out good each day though. Robyn OHSU From dellav <@t> musc.edu Tue Apr 26 15:00:14 2005 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] viability of placenta Message-ID: in order to provide an answer it would be most beneficial to understand what testing you'd be doing on refrigerated placentas. for example, we have found that for cytogenetics we still have viability after 48 hours refrigeration but of course it steadily drops off. for routine histology one can easily refrigerate for 3 or 4 days and still get decent histology, possibly longer ( I haven't actually studied this) at my former facility placenta grossing was batched and done twice a week. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Bernadette Weston" 04/26/05 09:17AM >>> How long can a placenta be refrigerated and still be viable for testing as well as Histology? Bernadette Weston HT Histology Supervisor Barberton Citizens Hospital Barberton, OH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Tue Apr 26 15:06:14 2005 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Temp/Humidity Records? Message-ID: Glen, I feel your pain. however, I'm not sure what else an accreditation inspector could substitute and still be reasonably assured that the procedure you have in your manual is actually being followed. yes, we all know that you could easily 'fudge' the documentation and pass inspection, but hopefully, in the interest of true quality control, everyone understands the value of recording the actual temperature used for epitope retrieval and not just record the minimum acceptable temperature. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Dawson, Glen" 04/26/05 09:16AM >>> Just got back from vacation but I feel the need to comment on this. Although I haven't heard of this one, it would not surprise me. Documentation, documentation, documentation; it is the only thing between us and utter chaos...give me a break. Each day, my staff and I do our IHC runs that require heat. My instructions are very straight forward; unless the thermometer reads at least 97 degrees, the run cannot continue. If the bath isn't up to temp, the slides wait until it is. We check and check and check this same thermometer many times a day and the heat runs don't continue until the magic number is reached. Still, every day, we must lug the temperature log out and put in that entry. Maybe this is a bad example but I don't see how writing an entry in a temperature log for a reading we all know by heart is keeping the walls of this lab from crumbling down. At some point, doesn't the additional, meaningless documentations added onto the CAP inspection roster become just that, meaningless. I picture a person somewhere in a dark room racking his or her brain 24/7 for more "required" documentation for CAP inspections because every year I think that CAP couldn't possibly come up with more things to document, they do. I'm rambling, long story short, I'm sick of all this documentation. My Opinion, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Apr 26 16:12:59 2005 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Cleaning Acid (Long)... Aqua Regia Correction? References: <59C772E2D8EDF345AE1601F5B60B3CB50279CF@PHSXMB7.partners.org> Message-ID: <426EAEDB.682B03F9@uwo.ca> Sorry, my mistake. Well spotted. Aqua regia is, of course, 3 volumes of hydrochloric and one volume of nitric acid. (I have corrected it also in the quoted email below, just for posterity.) -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ "Due, Brice" wrote: > > Hello John, as always, thank you for sharing your knowledge. below you list aqua > regia as 3:1 nitric:hydrochloric. I have more often seen it as 1:3 > nitric:hydrochloric, and this is what I've used before. Was this a typo or can > it go both ways? > > Thanks, > -brice > Neuropathology Lab > Brigham & Women's Hospital, > Boston > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of John A. > Kiernan > Sent: Tuesday, April 26, 2005 12:29 PM > To: Scott, Allison D > Cc: 'histonet@lists.utsouthwestern.edu' > Subject: Re: [Histonet] Cleaning Acid (Long) > > An answer to a question asked last week about > cleaning glassware for a silver staining method. > Skip to the bottom line if you don't want to > know the reasons. > > Glassware that has been used for silver methods can > collect traces of metallic silver - sometimes enough > to see as a mirror or grey marks. If the glassware > is used again for silver staining, these traces serve > as nuclei for deposition of more silver, and they > are bigger than the desired nucleation sites in > the sections. The staining method may simply fail > or the chemical reaction may go wild, with nonspecific > deposition of silver all over the place. > > Silver is soluble in nitric acid. My cleaning technique > is to put a little concentrated HNO3 in the vessel > (Coplin jar or larger tank) and carefully move it over > all the inside surface, over a sink with running water > so that any spilled drops of acid are quickly diluted. > Visible silver (look in the corners) disappears > instantly, so smaller amounts must also be removed. > Pour the used nitric acid into a beaker containing > tap water (for later neutralization and disposal). If > the tap water becomes opalescent you have removed a > significant amount of silver from the glass. > > Next - and this is important - Fill the vessel with > PURE (eg distilled) water and empty it; do this twice > so that the concentration of silver ions in the water > adhering to the sides is infinitessimal. Tap water > must not be used for these washes because it always > contains anions (chloride, bicarbonate, others?) that > form insoluble silver salts. Any colloidal silver > chloride particles that stay on the glass will be > partly reduced to silver by exposure to light and > can be expected to provide nucleation sites in > later silver staining methods. Finally dry the > glassware by letting it drain and store the vessels > upside-down in a closed cupboard. > > Deposited silver is not the only kind of dirt that > can spoil silver staining. Any kind of organic > chemical deposit (such as a fragment of a section) > or even residue from evaporated tap water will > work in the same way. Concentrated nitric acid > quickly oxidizes and dissolves pretty well everything, > with one notable exception. > > The exception is metallic gold. This can replace > deposited silver in glassware used for gold-toning, > a procedure often used to improve contrast in > silvered preparations. Gold on glass may appear > only as a light purple discoloration. Any colour > that resists nitric acid is probably gold. It can be > removed with aqua regia, which is a 3:1 mixture of > concentrated hydrochloric:nitric acids. Make and > use aqua regia in a fume hood because it emits > fumes of chlorine and nitrogen oxides. I have > resorted to aqua regia 3 or 4 times (in >30 years) > to get rid of gold on glass. The obvious way to > prevent contamination is to reserve certain jars > and dishes for gold-toning and nothing else. This > is not very practical if we do many different > methods and do not have a cupboard big enough for > all jars that might carry catalytic contaminants. > > Some people use Farmer's reducer (a solution > containing potassium ferricyanide and sodium > thiosulphate). This is an altogether gentler > liquid than nitric acid and it can dissolve silver > from black & white photographs. The action of > Farmer's reducer on visibly discoloured glass is > very slow, and this mixture is not going to > destroy insoluble organic forms of dirt such > as bits of tissue. > > Bottom line: Nitric acid, followed by pure (not > tap) water. > > John Kiernan > London, Canada. > ________________________________ > "Scott, Allison D" wrote: > > > > Hello to all in histoland. I need help in locating a cleaning acid solution > > for cleaning glassware. We are having a problem with our GMS stain. I > > think it has something to do with the glassware. Thanks in advance > > Allison Scott > ------------------------- From anh2006 <@t> med.cornell.edu Tue Apr 26 16:53:30 2005 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Re: Distilled water rinse versus tap water rinsing, a curious thing In-Reply-To: <6.0.0.22.1.20050426122435.01b86e40@gemini.msu.montana.edu> References: <42f77b432f60.432f6042f77b@amc.uva.nl> <6.0.0.22.1.20050426122435.01b86e40@gemini.msu.montana.edu> Message-ID: Dear Gayle/Chris -- Very interesting indeed ... have you published these findings? It would be interesting to know the regional differences in such typically benign steps as washing after the protocol is complete! It is interesting to ponder that such steps could be responsible for such variations from lab to lab. I have found that for "delicate" sections - such as mouse hematopoietic tissue cryosections which have been fixed with acetone - that the crucial "make it or break it" step is what you blue in after hematoxylin counterstain. Classic ammonia water (0.25% ammonium hydroxide in dH20) seems to be incredibly damaging to sections so instead I use "Scott's Tap Water Substitute" from Sigma. I think someone else (maybe one of you guys) mentioned this recently as well on Histonet. Have a great week, Andrea At 12:48 PM -0600 4/26/05, Gayle Callis wrote: >Andrea and Chris, > >I have seen Chris's results on rinsing. We collaborated on this >after I got home from visiting his lab. I repeated his rinsing >experiments in my lab to see if I had the same problems. I did NOT >get the same results he observed. Could this be caused by >differences in another countries water and purification systems? >Very curious why this happens as rinsing with distilled water is a >very common practice. > >My experience with acetone or acetone/ethyl alcohol combo fixed >mouse frozen section IHC is the same as Andrea's, no problems or >damage to sections after either rinsing with MilliQ, RO or tap >water. Acetone fixed sections are less robust than the AA combo >fixed sections, but distilled water rinse was not a problem anyway. >We do not use NBF for murine IHC work here. -- From xavieranton <@t> yahoo.com Tue Apr 26 19:25:50 2005 From: xavieranton <@t> yahoo.com (Anton Xavier) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Unbiased feedback for a presentation: Message-ID: <20050427002550.11791.qmail@web54407.mail.yahoo.com> Dear All, I am currently doing some unbiased research into life science manufacturing companies for an upcoming presentation, focusing particularly on antibody manufacturing companies. I was wondering, as histotechnologists and immunocytochemists, what antibody companies do you prefer to buy from and why? Is it psychological: ie the first lab you worked in had those antibodies so you buy them now, or is it cost, or word of mouth, or unbelievably and annoyingly persistent antibody sales reps?? If glancing in your fridge or -20freezer approximately (in percentage terms) what companies are you're antibodies from?? (something like: Company X 40% Company Y 60% etc)? Please send replies to xavieranton@yahoo.com all emails sent personally to me will be kept strictly confidential. Thanks for all your help! Anton. From Jacqueline.Malam <@t> rli.mbht.nhs.uk Wed Apr 27 04:44:08 2005 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] RE: Histonet Digest, Vol 17, Issue 38 - Heinz body stain Message-ID: I have a Heinz body stain for you: Rhodinile blue (Simpson, Carlisle, Mallard 1970) Working solution: 0.5g rhodinile blue (E. Gurr, Michrome No 1156) to 100ml of a 1% solution of aqueous NaCl. Shake well and filter through No 1 Whatman filter paper. Method: To a few drops of heparinised or EDTA-treated blood in a small vial, add the same amount of working solution of stain. Swirl gently and stand for 2 mins (the stain - not you!!). Prepare thin smears and air dry. Examine as usual or mount in resin mountant. Results: Heinz bodies - deep purple Erythrocytes - yellow-orange to blue-green Reticulocytes - blue, although reticulum not evident unless staining time is prolonged. Method found in "Staining Procedures" George Clark, 4th ed. Hope it works - never tried it. Best of luck Jacqui -----Original Message----- From: histonet-request@lists.utsouthwestern.edu [mailto:histonet-request@lists.utsouthwestern.edu] Sent: 26 April 2005 18:01 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 17, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Heinz Body Stain (krat18@aol.com) 2. Re: Lyme Disease (Richard Cartun) ---------------------------------------------------------------------- Message: 1 Date: Tue, 26 Apr 2005 12:45:33 -0400 From: krat18@aol.com Subject: [Histonet] Heinz Body Stain To: histonet@lists.utsouthwestern.edu Message-ID: <8C718967FC22B1B-F98-C0F1@FWM-D40.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Our Hematology dept. asked us to develop a procedure for Heinz Body Stain. Does anyone have such a procedure that they could send us? Thanks for your help! Karen_Raterman@ssmhc.com ------------------------------ Message: 2 Date: Tue, 26 Apr 2005 12:46:36 -0400 From: "Richard Cartun" Subject: Re: [Histonet] Lyme Disease To: , Message-ID: Content-Type: text/plain; charset=US-ASCII I have IHC for Borrelia burgdorferi, but I have never seen a positive human case. I have obtained positive staining in animal tissue and, obviously, our positive control tissue (lung injected with B. burgdorferi). Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Laurie Colbert" 04/26/05 12:02PM >>> Will a warthin starry diagnose lyme disease? Is there an IHC stain that will stain for this? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------- Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 17, Issue 38 **************************************** DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. Morecambe Bay Hospitals NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From Jacqueline.Malam <@t> rli.mbht.nhs.uk Wed Apr 27 07:01:25 2005 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] RE: Histonet Digest, Vol 17, Issue 38 Lyme disease Message-ID: According to books, the spirochaetes are rarely demonstrable in tissues or body fluids, especially in the later stages of the disease, and you have to rely on specialized culture. Warthin Starry will stain spirochaetes; Levaditi's method would show them too. Jacqui -----Original Message----- From: histonet-request@lists.utsouthwestern.edu [mailto:histonet-request@lists.utsouthwestern.edu] Sent: 26 April 2005 18:01 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 17, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Heinz Body Stain (krat18@aol.com) 2. Re: Lyme Disease (Richard Cartun) ---------------------------------------------------------------------- Message: 1 Date: Tue, 26 Apr 2005 12:45:33 -0400 From: krat18@aol.com Subject: [Histonet] Heinz Body Stain To: histonet@lists.utsouthwestern.edu Message-ID: <8C718967FC22B1B-F98-C0F1@FWM-D40.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Our Hematology dept. asked us to develop a procedure for Heinz Body Stain. Does anyone have such a procedure that they could send us? Thanks for your help! Karen_Raterman@ssmhc.com ------------------------------ Message: 2 Date: Tue, 26 Apr 2005 12:46:36 -0400 From: "Richard Cartun" Subject: Re: [Histonet] Lyme Disease To: , Message-ID: Content-Type: text/plain; charset=US-ASCII I have IHC for Borrelia burgdorferi, but I have never seen a positive human case. I have obtained positive staining in animal tissue and, obviously, our positive control tissue (lung injected with B. burgdorferi). Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Laurie Colbert" 04/26/05 12:02PM >>> Will a warthin starry diagnose lyme disease? Is there an IHC stain that will stain for this? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------- Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 17, Issue 38 **************************************** DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. Morecambe Bay Hospitals NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From NMargaryan <@t> childrensmemorial.org Wed Apr 27 09:13:39 2005 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Re: Distilled water rinse versus tap water rinsing, a curious thing Message-ID: <63B8B599DE283148B92E83C78B32C15D8B5607@cmhexbe2.childrensmemorial.org> Dear Gayle/Chris/Andrea, I usually use hematoxylin Mayer's as a counterstain and after wash with H2O, 95%Alcohol, 2x100% Alcohol and Xyline for immuno-. The "Scott's Tap Water" and bluing solution I use for H&E only. If it is wrong, please, let me know. Why you use "Scott's Tap Water' in immuno? Thanks, Naira -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper Sent: Tuesday, April 26, 2005 4:54 PM To: Gayle Callis; Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Distilled water rinse versus tap water rinsing,a curious thing Dear Gayle/Chris -- Very interesting indeed ... have you published these findings? It would be interesting to know the regional differences in such typically benign steps as washing after the protocol is complete! It is interesting to ponder that such steps could be responsible for such variations from lab to lab. I have found that for "delicate" sections - such as mouse hematopoietic tissue cryosections which have been fixed with acetone - that the crucial "make it or break it" step is what you blue in after hematoxylin counterstain. Classic ammonia water (0.25% ammonium hydroxide in dH20) seems to be incredibly damaging to sections so instead I use "Scott's Tap Water Substitute" from Sigma. I think someone else (maybe one of you guys) mentioned this recently as well on Histonet. Have a great week, Andrea At 12:48 PM -0600 4/26/05, Gayle Callis wrote: >Andrea and Chris, > >I have seen Chris's results on rinsing. We collaborated on this >after I got home from visiting his lab. I repeated his rinsing >experiments in my lab to see if I had the same problems. I did NOT >get the same results he observed. Could this be caused by >differences in another countries water and purification systems? >Very curious why this happens as rinsing with distilled water is a >very common practice. > >My experience with acetone or acetone/ethyl alcohol combo fixed >mouse frozen section IHC is the same as Andrea's, no problems or >damage to sections after either rinsing with MilliQ, RO or tap >water. Acetone fixed sections are less robust than the AA combo >fixed sections, but distilled water rinse was not a problem anyway. >We do not use NBF for murine IHC work here. -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments. From lfidgen <@t> vt.edu Wed Apr 27 09:30:26 2005 From: lfidgen <@t> vt.edu (Laura Fidgen) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] S3 Histo Message-ID: <6.0.0.22.0.20050427102225.025806a0@pop.vt.edu> We are trying to implement the use of a xylene substitute, namely, S3 Histo from BBC Biomedical. Can anyone give me information on whether processing times need to be adjusted? We currently use xylene on our processor (2 stations x 1 hour each). Also, we are going to use the substitute in our automated Leica stainer. Would the station times for clearing need to be adjusted? Thanks in advance for any responses! Laura L. Fidgen, MScF, BSc, HT(ASCP) Laboratory and Research Practioner VMRCVM, Histopatholgy/Clinical Pathology Dept. Blacksburg, VA 24060-0443 From anh2006 <@t> med.cornell.edu Wed Apr 27 09:41:02 2005 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Re: Distilled water rinse versus tap water rinsing,a curious thing In-Reply-To: <63B8B599DE283148B92E83C78B32C15D8B5607@cmhexbe2.childrensmemorial.org> References: <63B8B599DE283148B92E83C78B32C15D8B5607@cmhexbe2.childrensmemorial.org> Message-ID: Dear Naira, My institution's tap water does not blue my hematoxylin counterstain to my satisfaction so this is why I prefer to use a blueing reagent for immunos. I like my hematoxylin counterstain for IHC to be *really really* blue so I need a blueing solution beyond that of tap water to accomplish that. In addition, when doing quantitation based on color gradations I wanted to install some sort of quality control and reproducibility in the counterstaining and I found that using a commercial reagent was better for that. I found the ammonia water we were using for paraffin H&Es to be very harsh on my frozens so now I use the Scott's. If your tap water blues appropriately I would keep using that it is much cheaper (aka free). Also as Gayle mentioned there are many other cheaper sources of reagents similar to Scott's which work just as well. Andrea At 9:13 AM -0500 4/27/05, Margaryan, Naira wrote: >Content-Type: text/html >Content-Transfer-Encoding: 7bit >X-NAIMIME-Modified: 1 > >Dear Gayle/Chris/Andrea, > > I usually use hematoxylin Mayer's as a counterstain and after wash >with H2O, 95%Alcohol, 2x100% Alcohol and Xyline for immuno-. > >The "Scott's Tap Water" and bluing solution I use for H&E only. > >If it is wrong, please, let me know. Why you use "Scott's Tap Water' >in immuno? > > >Thanks, >Naira > -- From TillRenee <@t> uams.edu Wed Apr 27 10:31:17 2005 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] CD markers on pig Message-ID: Has anyone done any CD markers on pig tissue? We are wanting to do fluorescence for CD45, CD3, CD4, CD21, and CD8 on cryosections of gi. Also, any Ki-67 that will work on pig. Renee' Till, HT Arkansas Childrens Nutrition Center 1120 Marshall Little Rock, AR 72202 (501) 364-2774 From Jacqueline.Malam <@t> rli.mbht.nhs.uk Wed Apr 27 11:00:38 2005 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] RE: Histonet Digest, Vol 17, Issue 37 Message-ID: Hi I had the same problem, especially with fatty tissues and found that poly-l-lysine (SIGMA, code P-1524) used at 0.1% aqueous and spread onto a slide as you would a blood smear worked much better. Cheers Jacqui Lancaster -----Original Message----- From: histonet-request@lists.utsouthwestern.edu [mailto:histonet-request@lists.utsouthwestern.edu] Sent: 26 April 2005 17:42 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 17, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. GFP again (Caroline Bass) 2. paraffins (Steven Coakley) 3. RE: GFP again (Favara, Cynthia (NIH/NIAID)) 4. Immunofluorescence about cartilage (=?gb2312?q?=CC=EC=20=D0=C1?=) 5. reply to murine CD4 and CD8 in brain sections (Gayle Callis) 6. Re: paraffins (John Kiernan) 7. GFP, the ongoing dilemma with DsRed included (Gayle Callis) 8. First time with frozen sections, long reply (Gayle Callis) 9. Surgical Volume vs. number of tests (Patricia Karlisch) 10. RE: Surgical Volume vs. number of tests (Bonner, Janet) 11. Surgical Volume vs. number of tests (Stephen Peters M.D.) 12. NSH Self Assessment and Study Guide CD (Dick Paulson [Source Medical Products]) 13. coating slides for paraffin sections of CNS tissue (Julia Edgar) 14. RE: need some help with frozen sections (C.M. van der Loos) 15. immuno-LCM (Garry Ashton) 16. RE: coating slides for paraffin sections of CNS tissue (Favara, Cynthia (NIH/NIAID)) 17. viability of placenta (Bernadette Weston) 18. RE: Temp/Humidity Records? (Dawson, Glen) 19. position (Dorothy.L.Webb@HealthPartners.Com) 20. RE: coating slides for paraffin sections of CNS tissue (Due, Brice) 21. RE: Temp/Humidity Records? (Willis, Donna) 22. temp/humidity (Linda Blazek) 23. Lyme Disease (Laurie Colbert) 24. Re: Cleaning Acid (Long) (John A. Kiernan) ---------------------------------------------------------------------- Message: 1 Date: Mon, 25 Apr 2005 13:21:41 -0400 From: Caroline Bass Subject: [Histonet] GFP again To: " " Message-ID: <971ab99ff4f9d5a18b6bcaf122da48b6@bidmc.harvard.edu> Content-Type: text/plain; charset=US-ASCII; format=flowed Hey Guys, I have been searching the internet and all sorts of forums, including histonet trying to find the answers to my GFP questions. Essentially, I have a viral vector that I would like to inject into the liver which expresses EGFP. I would like to have the easiest way to determine if it works or not. I have heard that it is virtually impossible to detect native fluorescence with GFP, and that frozen cryostat sections completely lose the signal. Am I missing something here? There is a paper that is essentially doing the same thing that I would like to do, and they have enough signal that they can see it in the intact animal with a UV lamp, or in 6 micron cryosections without fixation or perfusion. According to everything I read, this shouldn't work. Does EGFP produce a stronger signal that can be seen in this way? The paper is: Nature Biotechnol 2005 Mar; 23(3):321-8. Any suggestions on how to process the liver such that I can see the native EGFP signal would be greatly appreciated. I have access to a cryostat and a microtome. I can freeze the tissue, perfuse the mouse, whatever it takes. I would prefer not to use immunostaining initially, as I have to section through entire lobes to visualize where the virus diffuses, etc. I can use an antibody once I get a rough estimate of where the signal is, but we don't have enough money to immunostain every section. Thanks, Caroline Bass ------------------------------ Message: 2 Date: Thu, 21 Apr 2005 12:52:48 -0500 From: "Steven Coakley" Subject: [Histonet] paraffins To: Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have any experience with surgpath IF200 infiltration medium and EM400 embedding medium. I'm considering using 2 different paraffins instead of one of the duel use paraffins. Is infiltation really improved by using the "softer" paraffin. Any opinions recommended. I'm starting up a new histo lab. Thanks, Steven ------------------------------ Message: 3 Date: Mon, 25 Apr 2005 13:55:57 -0400 From: "Favara, Cynthia (NIH/NIAID)" Subject: RE: [Histonet] GFP again To: 'Caroline Bass' , " " Message-ID: Content-Type: text/plain Caroline, I am pretty much a novice at the GFP but have done some work on this. I can tell you that the protein is very soluble and does not survive standard fixation with NBF when working with frozen sections. There is a nice paper J Histochem Cytochem. 2003 Mar;51(3):401-4 that discusses this and proposes a vapor fixation. We have had good luck with formalin fixed paraffin embedded tissue using anti GFP antibodies. In my hands there is considerable variation in the sensitivity and signal /noise ratio. My suspicion is that due to the solubility of the protein perfused specimens may be preferable. But I have not tested this. We have started a project with grafting of GFP expressing tissue into a non GFP expressing mouse and I can tell you that seeing the GFP in the fresh tissue is not as obvious as some of the literature would lead one to believe. I am still working on this so I look forward to replies from others more knowledgeable and experienced than I. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Caroline Bass [mailto:cbass@bidmc.harvard.edu] Sent: Monday, April 25, 2005 10:22 AM To: Subject: [Histonet] GFP again Hey Guys, I have been searching the internet and all sorts of forums, including histonet trying to find the answers to my GFP questions. Essentially, I have a viral vector that I would like to inject into the liver which expresses EGFP. I would like to have the easiest way to determine if it works or not. I have heard that it is virtually impossible to detect native fluorescence with GFP, and that frozen cryostat sections completely lose the signal. Am I missing something here? There is a paper that is essentially doing the same thing that I would like to do, and they have enough signal that they can see it in the intact animal with a UV lamp, or in 6 micron cryosections without fixation or perfusion. According to everything I read, this shouldn't work. Does EGFP produce a stronger signal that can be seen in this way? The paper is: Nature Biotechnol 2005 Mar; 23(3):321-8. Any suggestions on how to process the liver such that I can see the native EGFP signal would be greatly appreciated. I have access to a cryostat and a microtome. I can freeze the tissue, perfuse the mouse, whatever it takes. I would prefer not to use immunostaining initially, as I have to section through entire lobes to visualize where the virus diffuses, etc. I can use an antibody once I get a rough estimate of where the signal is, but we don't have enough money to immunostain every section. Thanks, Caroline Bass _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 26 Apr 2005 01:58:05 +0800 (CST) From: =?gb2312?q?=CC=EC=20=D0=C1?= Subject: [Histonet] Immunofluorescence about cartilage To: Histonet@pathology.swmed.edu Message-ID: <20050425175805.63358.qmail@web15506.mail.cnb.yahoo.com> Content-Type: text/plain; charset=gb2312 Hello,all, I am doing double immunofluorescence in bone sections. One protein was mainly expressed in cytoplasm, but we found that some positive expressions appeared among chondrocytes, moreover their expressions were stronger, my teacher told me, it could be due to this condition that part cartilages were broken during the staining so that this protein came out from chondrocytes, then positive expressions were present among chondrocytes. If it is true, what should I do to avoid this situation? If you have some experience about it, please do me a favor! Thank you! In addition, which methods can be used to reduce background? Guofeng Qian --------------------------------- Do You Yahoo!? ?????????????????????????????? ------------------------------ Message: 5 Date: Mon, 25 Apr 2005 12:16:02 -0600 From: Gayle Callis Subject: [Histonet] reply to murine CD4 and CD8 in brain sections To: Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050425120037.01b0cba8@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Christophe Meiers wrote: Could someone please give advice regarding cd4 and cd8 staining of brain tissue. We dissected out the brain of a mouse, made longitudinal section midbrain, one half of the brain was placed in formalin for routine H & E, the other half was snap frozen for the cd4 and cd8. The sections came out absolutely horrible with what looked like exploded tissue. Should the brain be treated in a different fashion ? Would formalin interfere with staining CD4 and CD8 ? *****You will have to frozen sections on fresh, unfixed snap frozen brain. Brain should be embedded in OCT, then it must MUST be snap frozen at very cold temps with either a dry ice/2 methylbutane mixture or another adequate method. Snap freezing has been discussed many times on Histonet, for methods do a search of Histonet Archives at www.histosearch.org. Formalin totally kills murine CD4 and CD8 antigen, and no retrieval or enzyme digestion will ever recover the antigens. Make sure block temperature and knife temperature are the same, equilibrate block (if stored at -80C) 20 min or so before cutting. Too cold a block means crunchy sections. Cryosection brain at -16C, 5 um sections, pick up on Plus Charge slides, air dry overnight, fix in 75%acetone/25% absolute ethanol (no substitute on alcohol can be used) for 5 min at room temperature, go directly to a buffer rinse FROM the fixative, 3 changes of buffer, and proceed with staining. Do not store your frozen sections in cryostat after cutting, go directly to air drying. We put our sections over a 16 mesh silica gel to ensure dryness. Some people use acetone fixation at 4C for 10 min, but the acetone/alcohol improves morphology and keeps excellent immunostaining. Peroxidase block, DAKO S2001 for 10 min Normal serum block for 30 min (10% goat/2.5%mouse in TBS or Dulbeccos PBS with 0.05% Tween 20. We add 0.2% goat serum to rinse buffers along with 0.05% Tween 20 Avidin/biotin block kit Vector CD4 Rat antimouse BD Pharmigen 1:500 (0.5mg/ml conc) in 5% goat serum. CD8 Rat antimouse 1:200 (0.5mg/ml conc) in 5% goat serum IgG isotype matched controls for these antibodies at same concentration in ug/ml is negative control Incubate 30 min, RT Goat antiRat F(ab')2 frag of IgG (0.5mg.ml) diluted 1:250 in normal serum block (yes! with mouse serum) for 30 min Strepavidin-HRP 1:500 from Biosource/TAGO diluted in rinse buffer, 20 min AEC+ DAKO control color development of positive control (normal spleen works) for approx 3 min Rinse, counterstain, and mount with aqueous mounting media. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 6 Date: Mon, 25 Apr 2005 14:33:16 -0400 From: John Kiernan Subject: Re: [Histonet] paraffins To: steven.coakley@mirusbio.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <426D37EC.6949BC0E@uwo.ca> Content-Type: text/plain; charset=us-ascii The subject of different paraffins was discussed at some length on Histonet more than 5 years ago. The most authoritative comments seemed to be from Russ Allison. His principal conclusion was that ingredients other than the hydrocarbons (the wax itself) made no difference to cutting properties. Russ has published in this field; the following list is probably incomplete. Allison RT (1978) The crystaline nature of histology waxes: a preliminary communication. Medical Laboratory Sciences 35: 355-363. Allison RT (1979) The crystaline nature of histology waxes: the effects of microtomy on the micro-structure of paraffin wax in sections. Medical Laboratory Sciences 36: 359-372. Allison RT, Lloyd D (1996) Measuring infiltration during paraffin wax processing for histology. British Journal of Biomedical Science 53: 235-237. Allison RT, Bryant D (1998) Effects of processing at 45C on staining. Biotechnic & Histochemistry 73: 128-136. Allison RT (2002) Tissue processing and sectioning. Chapter 7 in Microscopy and Histology for Molecular Biologists (Kiernan JA & Mason I eds). London: Portland Press. pp 144-169. In the most recent of these publications, Russ concedes that there may be some circumstances where addition of DMSO or a synthetic polymer could be advantageous. Unfortunately the archives at www.histosearch.com do not include the older correspondence about waxes. Russ Allison does not seem to be around on Histonet these days; a pity! -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Steven Coakley wrote: > > Does anyone have any experience with surgpath IF200 infiltration medium and > EM400 embedding medium. I'm considering using 2 different paraffins instead > of one of the duel use paraffins. Is infiltation really improved by using > the "softer" paraffin. Any opinions recommended. I'm starting up a new > histo lab. > > Thanks, > > Steven > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 25 Apr 2005 12:42:39 -0600 From: Gayle Callis Subject: [Histonet] GFP, the ongoing dilemma with DsRed included To: Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050425121642.01b40a58@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Dear Caroline, You wrote: I have been searching the internet and all sorts of forums, including histonet trying to find the answers to my GFP questions. Essentially, I have a viral vector that I would like to inject into the liver which expresses EGFP. I would like to have the easiest way to determine if it works or not. I have heard that it is virtually impossible to detect native fluorescence with GFP, and that frozen cryostat sections completely lose the signal. Am I missing something here? There is a paper that is essentially doing the same thing that I would like to do, and they have enough signal that they can see it in the intact animal with a UV lamp, or in 6 micron cryosections without fixation or perfusion. According to everything I read, this shouldn't work. Does EGFP produce a stronger signal that can be seen in this way? The paper is: Nature Biotechnol 2005 Mar; 23(3):321-8. Any suggestions on how to process the liver such that I can see the native EGFP signal would be greatly appreciated. I have access to a cryostat and a microtome. I can freeze the tissue, perfuse the mouse, whatever it takes. I would prefer not to use immunostaining initially, as I have to section through entire lobes to visualize where the virus diffuses, etc. I can use an antibody once I get a rough estimate of where the signal is, but we don't have enough money to immunostain every section. ***We recently undertook a similar project with undecalcified murine nasal turbinate and small intestine tissue sections from mouse. In order to do immunofluorescence staining for a CD marker or dendritic cell, we were required to fix with acetone or acetone/alcohol, which immediately killed the eGFP and it failed to glow. This was also going be a problem with DsRED, a bioluminescent protein from Coral rather than jellyfish. One major problem is increasing autofluorescence in tissues by using formalin or paraformaldehyde fixatives. We could never get the best of both worlds with eGFP or DsRed. Final solution to the problem: With DsRed, unfixed fresh snap frozen tissues were cryosectioned at 5 um, mounted on Erie Plus Gold slides (a plus charged surface for extra holding power),and air dried for 30 min or more in front of a fan at RT. Air dried sections were rinsed one slide at time to prevent unfixed section loss with 2 changes PBS gentle agitation. We wanted to get rid of OCT. A coverslip was mounted with Prolong Gold antifade mounting media, ready to use containing DAPI for DNA/RNA. Results: spectacular DsRed where it was supposed to be, with nuclei counterfluorescing a lovely blue from DAPI staining DNA/RNA. The mounting media is a hard set. The only autofluorescence is natural, but does NOT interfere with the brighter signal of DsRed, and would not interfere with eGFP either. We are going to do this with eGFP next week to have green fluorescence with the DAPI stained nuclei. I have a review of autofluorescence I am going to attach to you. For more on eGFP and DsRed, go to Clontech website and download their Living Colours Manuals for these proteins. When you get to immunostaining, say for for a CD marker along with eGFP, we did our acetone/alcohol fixation, but detected eGFP with Goat antiGFP from Rockland (a superb antibody that is NOT expensive!) and came back with Donkey antiGoat-FITC, along with a biotinylated CD marker antibody and Strepavidin Alexa 555. This worked well for double fluorescence with the CD marker. As for DsRed, the antiDsRed antibody is pricey, so doing IFA staining for GFP has been more cost effective. ------------------------------ Message: 8 Date: Mon, 25 Apr 2005 13:02:41 -0600 From: Gayle Callis Subject: [Histonet] First time with frozen sections, long reply To: Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20050425124550.01b4c780@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed You wrote: I am starting to stain frozen sections for the first time. Until now I have only stained paraffin embedded sections and so was hoping for some pointers.I contacted our histopathology lab who provided me with the following information: They don't use any antigen retrieval method. They pretreat the slides only with methanol for 10min followed by drying before staining. Then they proceed with the normal steps of staining and dehydration. I have however read that acetone can be used instead of the methanol. What is the difference that this provides? I am trying to stain for Stat5. Is there anything els I should keep in mind when handling frozen sections? Questions: human tissue, animal tissue? Is the tissue fresh, unfixed or prefixed prior to snap freezing? Have you snap frozen (needed to minimize freezing artifact from water ice crystal formation) the tissues rather than cryostat freezing (too warm)? What antigens do you want to stain for? Fixation is dependent on the antigen, some will be fine with acetone, some may work better with another fixative. Antigen retrieval on frozen sections of fresh unfixed tissues is not needed as long a formalin or even paraformaldehyde is avoided. In general, AR tends to damage FS. If you plan to do CD marker staining, methanol can cause poor staining of these due to methanolic bridge formation. Avoid methanol as a fixative or in peroxidase block, use DAKO S2002 peroxidase block designed for gentle endog perox blocking, it doesn't contain MEOH and wil not chew section off the slide. Hydrogen peroxide can be put into PBS instead. Antibodies also need to be optimized since FS permit lower conc of antibodies. Dilution panels should be done. What does the spec sheet or manufacturer recommend for fixation of FS for your STAT5 antibody? You can always call their tech services and ask. Be gentle, FS are more fragile, and air dry them AFTER sectioning, DO NOT STORE newly cut setions in a cryostat. Avoid water condensation and freeze thawing of sections - this can damage antigens. Air drying overnight in front of a fan, or over dessicant is a good way to insure properly dried sections. Check Histonet Archives www.histosearch.org, it has tons of info on HOW to do frozen sections, fixation, blocking, and staining. Chris van der Loos has provided many excellent replies on frozen section IHC. DakoCytomation website has Immunochemical Staining Methods Handbook, 3rd Edition with tons of hints on what you want to do. Good luck Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) ------------------------------ Message: 9 Date: Mon, 25 Apr 2005 17:49:21 -0400 From: "Patricia Karlisch" Subject: [Histonet] Surgical Volume vs. number of tests To: Message-ID: Content-Type: text/plain; charset=US-ASCII Histonetters: First, thank you all for the wonderful responses that you sent regarding the immunohistochemical staining relative to the volume of stains done per day. Your responses were very helpful. I would like to pose another question to you. Do you think there is over utilization with ordering immunohistochemistries on a daily basis? Relative to your surgical cases what would you say that your percentage (or actual numbers) of immunohistochemical cases are and how many slides would that equate to. For example: 6-8% of all our cases require immunohistochemistry, thus as volume increases so do our immuno stains. We do on average 1400 immuno slides/month which includes surgical, biopsy, bone marrow, Cytology FNA's and skins with one tech on rotation and two stainers with a capacity of 40 slides total. (Our yearly surgicals are about 34000 cases). We do very little research in this lab and minimal duplicate staining for file, study or education. Do any of you employ a method to discourage over utilization of immuno staining? Thank you again for any advice you can give me. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu ------------------------------ Message: 10 Date: Mon, 25 Apr 2005 18:18:45 -0400 From: "Bonner, Janet" Subject: RE: [Histonet] Surgical Volume vs. number of tests To: "'Patricia Karlisch'" , histonet@lists.utsouthwestern.edu Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB424D@fh2k093.fhmis.net> Content-Type: text/plain; charset="iso-8859-1" The workload/slides seems in line with ours. The only discouraging factor to ordering certain immunos is the ability to charge the patient/insurance company AND to justify the order/charges if called on the carpet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patricia Karlisch Sent: Monday, April 25, 2005 5:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Surgical Volume vs. number of tests Histonetters: First, thank you all for the wonderful responses that you sent regarding the immunohistochemical staining relative to the volume of stains done per day. Your responses were very helpful. I would like to pose another question to you. Do you think there is over utilization with ordering immunohistochemistries on a daily basis? Relative to your surgical cases what would you say that your percentage (or actual numbers) of immunohistochemical cases are and how many slides would that equate to. For example: 6-8% of all our cases require immunohistochemistry, thus as volume increases so do our immuno stains. We do on average 1400 immuno slides/month which includes surgical, biopsy, bone marrow, Cytology FNA's and skins with one tech on rotation and two stainers with a capacity of 40 slides total. (Our yearly surgicals are about 34000 cases). We do very little research in this lab and minimal duplicate staining for file, study or education. Do any of you employ a method to discourage over utilization of immuno staining? Thank you again for any advice you can give me. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 25 Apr 2005 18:24:33 -0700 (PDT) From: "Stephen Peters M.D." Subject: [Histonet] Surgical Volume vs. number of tests To: Histonet@lists.utsouthwestern.edu Message-ID: <20050426012433.21115.qmail@web30410.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii Dear Pat, I will offer a pathologists point of view. The actual percent cases which will need immuno will vary depending on several factors. First the case mix. You are in an academic center. I would expect you would have a larger percentage of demanding cases than a small private hospital. For example if you did only hernias, gallbladders and seb kers, you would not need any immunos. If you are doing all lymphomas and and soft tissue tumors you would have a %100 immuno rate. Experience and degree of specialization of the pathologists will play a role. In many situations the less experienced people tend to order a bit more than the more experienced people because of the lack of confidence that comes with experience. This is a natural evolution of the life of the pathologist much as your experience has tought you to find the simplest path. More academic pathologists with subspecialties may study certain cases in more depth because of their fields of interest. Also being an academic center with residents pathologists will often order more complete panals even though the diagnosis is clear for the benifit of the residents experience. Taking those facts out of the equation, I would be surprised if everyone reading this has not experienced increasing percentages of immuno requests over the past years. The number of antibodies required to carry out what would be considered " standard of practice" by our friends in the legal profession has grown enormously. And they will continue to grow. They are extremely helpful and represent one of the most significant advances in our profession. But have no fear. I predict the day is coming when your percentage of immunos will start to creep down only to be replaced by more specific, more intellectually demanding, and probably more expensive molecular methods! As far as trying to discourage over utilization, that is a budget vs. medical care issue and is under the perview of your medical director. It is his job to optimize care while keeping to the budget. You would have to be an experienced surgical pathologist to even begin make this judgement. One thing you could do to see if there is a particular culprit is to audit the ordering patterns of the different pathologists. Your computer system may be able to give you this easily.If you believe somebody is being wasteful with ordering of studies it is your job to bring it up with your superiors. When it works its way up, it can be looked at by a someone who can evaluate the situation from both sides. If there is an undo amout of tests being ordered this could possibly be reduced by more intradepartmental consultation. Sometimes sharing cases before immunos are ordered can prevent over ordering. It can also backfire and end up getting you more requests! When I show one of my cases to colleagues invariably I end up ordering a few more stains to satisfy their curiosity. If the hospital is pressuring you from the budget side then take a good hard look at where you can save without jeopardizing patient care. I would rather them take away the heat and the toilet paper than force me take guesses by cutting back on necessary studies. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com ------------------------------ Message: 12 Date: Tue, 26 Apr 2005 02:24:19 -0500 From: "Dick Paulson [Source Medical Products]" Subject: [Histonet] NSH Self Assessment and Study Guide CD To: Message-ID: Content-Type: text/plain; charset="us-ascii" For those of you who ordered the CD but couldn't get it to work.. Good news. NSH sent me a CD to test. This is my response. The CD works on all the systems I tested. I tested on the following operating systems: Windows 98 Windows 2000 Windows XP Home Edition Windows XP Professional Edition All the operating systems have the most up to date Service Packs and updates applied. I tested with the disk in the CD drive and also created a folder and copied the contents of the CD to a folder I created on disk. I also created a folder on a Windows 2000 server with the CD contents and ran the program from a mapped drive to the server. I went through 2 Studies and 1 Test to completion. Everything works. This is a great learning tool. I hope this helps. The CD was sent on the 19th but I just got it today (25th). If you have any questions, please do not hesitate to contact me directly. Thank you, Source Medical Products Dick Paulson dpconsult@earthlink.net Phone (800) 393-6345 ------------------------------ Message: 13 Date: Tue, 26 Apr 2005 09:50:05 +0100 From: "Julia Edgar" Subject: [Histonet] coating slides for paraffin sections of CNS tissue To: Message-ID: <000901c54a3c$f17e3300$cdead182@vet.gla.ac.uk> Content-Type: text/plain; charset="iso-8859-1" Dear All We've been using APES coated slides for collecting paraffin embedded CNS tissue for immunohistochemistry (with microwave treatment) but the sections are becoming unstuck, to some extent, during processing. I will be grateful if you can tell me what you are coating slides with for small pieces of CNS tissue. Thank you Julia Julia Edgar BSc (Hons), PhD University of Glasgow Tel: 0141 330 5818 e-mail: je22r@udcf.gla.ac.uk ------------------------------ Message: 14 Date: Tue, 26 Apr 2005 11:01:54 +0200 From: "C.M. van der Loos" Subject: [Histonet] RE: need some help with frozen sections To: histonet@lists.utsouthwestern.edu Message-ID: <42f77b432f60.432f6042f77b@amc.uva.nl> Content-Type: text/plain; charset="us-ascii" Eva, Some DO's and DON'T's with immunostaining of cryostat tissue sections: * After cutting, let the sections dry overnight at room temperature under a ventilator * Fix with cold acetone (10 min, 4C). Some people apply a "double acetone fixation" method: 2x 10 min acetone fixation with air-drying in between. This may improve the tissue morphology. * The quality of the acetone should be P.A. grade and don't use it twice. Re-distilled acetone cannot be used for fixation of cryo's. Traces of water in the acetone ruins the tissue morphology. * Be aware that acetone is not a real fixative like NBF. Acetone just solves the fatty membranes and coagulate the proteins. Cryostat tissue sections remain quite vulnerable with respect to surfactants like Triton-X100, Tween-20. These should be avoided. Be aware that in some autostainer washbuffers surfactants may be included. * To solve this problem of tissue vulnerability an extra fixation (after acetone-fixation and air-drying) with Zamboni's (1 min, RT) is optional. This extra fixation step also improves the tissue morphology. However, depending on the antigen to be IHC stained, this step may also decrease the IHC staining intensity. * The application of methanol, either as a fixative or as base for endogenous peroxidase activity blocking is (at least to my vision) absolutely excluded. For example, most human CD markers are completely destroyed by methanol. On the other hand I am aware of investigators who are using successfully an acetone/methanol mixture for fixation of mouse cryo's for staining CD4, CD8 etc. * Acetone is not a good fixative when staining nuclear antigens. This fixation will lead to quite fuzzy stained nuclei. Instead, a NBF-fixation (5 min, RT) works out well. Although NBF-fixation is used: do NOT apply heat-induced antigen retrieval. * Some antigens associated with fatty structures (for example, oxLDL, or apoB) will solve into acetone. In this case NBF (5 min) can be tested. Again, NO antigen retrieval! * Endogenous peroxidase activity can be blocked with 0.1% Na-azide + 0.3% peroxide in PBS or TBS (20 min, RT). This kills at least the endogenous peroxidase activity in erythrocytes effectively, but in neutrophils some will remain. If endogenous peroxidase activity of neutrophils is a real problem, glucose oxidase blocking method is optional (see Histonet archives). * At the end of the IHC staining procedure, after the chromogen step wash your slides with tap water. NEVER use distilled water as this will ruin the tissue section completely!!!! * Unlike with FFPE sections, aqueous solutions (depending on the chromogen used of course) is no problem for mounting cryostat tissue sections. * The concept of first cutting a tissue section and than fixation means "post-fixation". This is something different from a FFPE section that is first fixed as a block, than embedded and cut ("pre-fixation"). This means that soluble antigens may leak away from a "post-fixed" tissue section. Be aware of this when IHC staining any small protein (<50KD), cytokine, chemokine, hormone, etc. I hope this isn't too much frightening you. Happy staining! Chris van der Loos, PhD Dept. of Pathology Academical Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 fax: +31 20 6960389 e-mail: [1]c.m.vanderloos@amc.uva.nl ----- Original Message ----- From Eva C Anderson Date Mon, 25 Apr 2005 09:59:34 -0400 To histonet@lists.utsouthwestern.edu Subject [Histonet] need some help with frozen sections Good morning, I am starting to stain frozen sections for the first time. Until now I have only stained paraffin embedded sections and so was hoping for some pointers. I contacted our histopathology lab who provided me with the following information: They don't use any antigen retrieval method. They pretreat the slides only with methanol for 10min followed by drying before staining. Then they proceed with the normal steps of staining and dehydration. I have however read that acetone can be used instead of the methanol. What is the difference that this provides? I am trying to stain for Stat5. Is there anything els I should keep in mind when handling frozen sections? Would be very grateful for any information you could provide this rookie with. Thank you, Eva References 1. mailto:c.m.vanderloos@amc.uva.nl ------------------------------ Message: 15 Date: Tue, 26 Apr 2005 11:14:18 +0100 From: "Garry Ashton" Subject: [Histonet] immuno-LCM To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear all, I'm currently trying to re-optimize an immuno technique on frozen sections that allows for the production of quality RNA after laser capture. Presently I have found that not allowing the sections to dry, storing at -80, and using a much shortened immuno method (2 mins max in all ab incubations), plus a shortened colour reaction, with an RNase inhibitor added at the ab and colour steps gives me reasonable results. Does anybody else in histoland use this technique regularly, and if so have they found results similar to me. Many thanks. Garry PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the Christie Hospital NHS Trust. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ Message: 16 Date: Tue, 26 Apr 2005 09:05:54 -0400 From: "Favara, Cynthia (NIH/NIAID)" Subject: RE: [Histonet] coating slides for paraffin sections of CNS tissue To: 'Julia Edgar' , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain Julia, I work with primarily rodent CNS tissue and use Superfrost/Plus I get them from Fischer in the US Cat# 22-034-979 and very rarely have any tissue loss generally when I neglect to properly dry the slide. Hope this is helpful. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Julia Edgar [mailto:je22r@udcf.gla.ac.uk] Sent: Tuesday, April 26, 2005 1:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coating slides for paraffin sections of CNS tissue Dear All We've been using APES coated slides for collecting paraffin embedded CNS tissue for immunohistochemistry (with microwave treatment) but the sections are becoming unstuck, to some extent, during processing. I will be grateful if you can tell me what you are coating slides with for small pieces of CNS tissue. Thank you Julia Julia Edgar BSc (Hons), PhD University of Glasgow Tel: 0141 330 5818 e-mail: je22r@udcf.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Tue, 26 Apr 2005 09:17:11 -0400 From: "Bernadette Weston" Subject: [Histonet] viability of placenta To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed How long can a placenta be refrigerated and still be viable for testing as well as Histology? Bernadette Weston HT Histology Supervisor Barberton Citizens Hospital Barberton, OH ------------------------------ Message: 18 Date: Tue, 26 Apr 2005 08:16:38 -0500 From: "Dawson, Glen" Subject: RE: [Histonet] Temp/Humidity Records? Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Just got back from vacation but I feel the need to comment on this. Although I haven't heard of this one, it would not surprise me. Documentation, documentation, documentation; it is the only thing between us and utter chaos...give me a break. Each day, my staff and I do our IHC runs that require heat. My instructions are very straight forward; unless the thermometer reads at least 97 degrees, the run cannot continue. If the bath isn't up to temp, the slides wait until it is. We check and check and check this same thermometer many times a day and the heat runs don't continue until the magic number is reached. Still, every day, we must lug the temperature log out and put in that entry. Maybe this is a bad example but I don't see how writing an entry in a temperature log for a reading we all know by heart is keeping the walls of this lab from crumbling down. At some point, doesn't the additional, meaningless documentations added onto the CAP inspection roster become just that, meaningless. I picture a person somewhere in a dark room racking his or her brain 24/7 for more "required" documentation for CAP inspections because every year I think that CAP couldn't possibly come up with more things to document, they do. I'm rambling, long story short, I'm sick of all this documentation. My Opinion, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI ------------------------------ Message: 19 Date: Tue, 26 Apr 2005 09:18:08 -0500 From: Dorothy.L.Webb@HealthPartners.Com Subject: [Histonet] position To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain We have a full time opening for a histotechnician, day shift, and on call every 7th Saturday between the hours of 0800 and 1430. This position rotates into all aspects of histology and will be trained to be a "back-up" in our IHC area. We are a large city trauma 1 hospital in St. Paul, MN. with 12 pathologists on board. A nice place to work, so, come on board!! You can contact me by Email if interested or go on line to regions.com for an application site and full job description! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ------------------------------ Message: 20 Date: Tue, 26 Apr 2005 10:49:03 -0400 From: "Due, Brice" Subject: RE: [Histonet] coating slides for paraffin sections of CNS tissue To: "Favara, Cynthia \(NIH/NIAID\)" , "Julia Edgar" , Message-ID: <59C772E2D8EDF345AE1601F5B60B3CB50279CA@PHSXMB7.partners.org> Content-Type: text/plain; charset="iso-8859-1" Hello Julia, I work in a clinical neuropathology lab. We do impox, silver stains, etc. on both charged (superfrost) and uncharged slides. We also do giant sctions up to 4in. x 6 in. on plain (uncharged) glass slides. We rarely have adhesion problems. You must make absolutely sure the sections are fully dried before you melt and deparaffinize them. If you melt the paraffin too soon, a water film will become trapped and the sections will begin lifting in xylene. The best way is to leave the slides in a 37C oven overnight. Second best is room temp overnight. In my experience, the second most important factor in adhesion is section flatness. Wrinkles will not only fill up with water, but they will also not make contact with the slide. In some autopsy cases we also use STA-ON (chromated gelatin) adhesive, either in the water bath, or smeared on the slide, but the drying rules above are still mandatory. If overnight is too long for you, first try it, and then try cutting back the drying times until your problems reappear. Good luck, -brice Neuropathology Lab Brigham & Women's Hospital Boston -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Favara, Cynthia (NIH/NIAID) Sent: Tuesday, April 26, 2005 9:06 AM To: 'Julia Edgar'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] coating slides for paraffin sections of CNS tissue Julia, I work with primarily rodent CNS tissue and use Superfrost/Plus I get them from Fischer in the US Cat# 22-034-979 and very rarely have any tissue loss generally when I neglect to properly dry the slide. Hope this is helpful. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Julia Edgar [mailto:je22r@udcf.gla.ac.uk] Sent: Tuesday, April 26, 2005 1:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coating slides for paraffin sections of CNS tissue Dear All We've been using APES coated slides for collecting paraffin embedded CNS tissue for immunohistochemistry (with microwave treatment) but the sections are becoming unstuck, to some extent, during processing. I will be grateful if you can tell me what you are coating slides with for small pieces of CNS tissue. Thank you Julia Julia Edgar BSc (Hons), PhD University of Glasgow Tel: 0141 330 5818 e-mail: je22r@udcf.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Tue, 26 Apr 2005 09:55:20 -0500 From: "Willis, Donna" Subject: RE: [Histonet] Temp/Humidity Records? To: "Dawson, Glen" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4483B6EE0FA82A4EAED2B1E161E5E41147C7BD@ftwex02.txhealth.org> Content-Type: text/plain; charset="us-ascii" Glen, Just wait until the CLSI (formally NCCLS) Microwave Device Use in the Histolog Laboratory Guideline is added to CAP. Donna Willis Histology Lab Manager Harris Methodist Fort Worth, Tx -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, April 26, 2005 8:17 AM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temp/Humidity Records? Just got back from vacation but I feel the need to comment on this. Although I haven't heard of this one, it would not surprise me. Documentation, documentation, documentation; it is the only thing between us and utter chaos...give me a break. Each day, my staff and I do our IHC runs that require heat. My instructions are very straight forward; unless the thermometer reads at least 97 degrees, the run cannot continue. If the bath isn't up to temp, the slides wait until it is. We check and check and check this same thermometer many times a day and the heat runs don't continue until the magic number is reached. Still, every day, we must lug the temperature log out and put in that entry. Maybe this is a bad example but I don't see how writing an entry in a temperature log for a reading we all know by heart is keeping the walls of this lab from crumbling down. At some point, doesn't the additional, meaningless documentations added onto the CAP inspection roster become just that, meaningless. I picture a person somewhere in a dark room racking his or her brain 24/7 for more "required" documentation for CAP inspections because every year I think that CAP couldn't possibly come up with more things to document, they do. I'm rambling, long story short, I'm sick of all this documentation. My Opinion, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. ------------------------------ Message: 22 Date: Tue, 26 Apr 2005 11:32:28 -0400 From: "Linda Blazek" Subject: [Histonet] temp/humidity To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Where in the CAP guidelines does it say that humidity must be documented? Linda Linda Blazek, HT (ASCP) Department of Pathology Children's Medical Center Dayton, Ohio 45404 (937) 641-3358 fax (937)641-5482 blazekl@childrensdayton.org ------------------------------ Message: 23 Date: Tue, 26 Apr 2005 09:02:22 -0700 From: "Laurie Colbert" Subject: [Histonet] Lyme Disease To: Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628001C5C2DC@EXCHANGE1.huntingtonhospital.com> Content-Type: text/plain; charset="iso-8859-1" Will a warthin starry diagnose lyme disease? Is there an IHC stain that will stain for this? Laurie Colbert ------------------------------ Message: 24 Date: Tue, 26 Apr 2005 12:28:58 -0400 From: "John A. Kiernan" Subject: Re: [Histonet] Cleaning Acid (Long) To: "Scott, Allison D" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <426E6C4A.D7D3D0A0@uwo.ca> Content-Type: text/plain; charset=us-ascii An answer to a question asked last week about cleaning glassware for a silver staining method. Skip to the bottom line if you don't want to know the reasons. Glassware that has been used for silver methods can collect traces of metallic silver - sometimes enough to see as a mirror or grey marks. If the glassware is used again for silver staining, these traces serve as nuclei for deposition of more silver, and they are bigger than the desired nucleation sites in the sections. The staining method may simply fail or the chemical reaction may go wild, with nonspecific deposition of silver all over the place. Silver is soluble in nitric acid. My cleaning technique is to put a little concentrated HNO3 in the vessel (Coplin jar or larger tank) and carefully move it over all the inside surface, over a sink with running water so that any spilled drops of acid are quickly diluted. Visible silver (look in the corners) disappears instantly, so smaller amounts must also be removed. Pour the used nitric acid into a beaker containing tap water (for later neutralization and disposal). If the tap water becomes opalescent you have removed a significant amount of silver from the glass. Next - and this is important - Fill the vessel with PURE (eg distilled) water and empty it; do this twice so that the concentration of silver ions in the water adhering to the sides is infinitessimal. Tap water must not be used for these washes because it always contains anions (chloride, bicarbonate, others?) that form insoluble silver salts. Any colloidal silver chloride particles that stay on the glass will be partly reduced to silver by exposure to light and can be expected to provide nucleation sites in later silver staining methods. Finally dry the glassware by letting it drain and store the vessels upside-down in a closed cupboard. Deposited silver is not the only kind of dirt that can spoil silver staining. Any kind of organic chemical deposit (such as a fragment of a section) or even residue from evaporated tap water will work in the same way. Concentrated nitric acid quickly oxidizes and dissolves pretty well everything, with one notable exception. The exception is metallic gold. This can replace deposited silver in glassware used for gold-toning, a procedure often used to improve contrast in silvered preparations. Gold on glass may appear only as a light purple discoloration. Any colour that resists nitric acid is probably gold. It can be removed with aqua regia, which is a 3:1 mixture of concentrated nitric:hydrochloric acids. Make and use aqua regia in a fume hood because it emits fumes of chlorine and nitrogen oxides. I have resorted to aqua regia 3 or 4 times (in >30 years) to get rid of gold on glass. The obvious way to prevent contamination is to reserve certain jars and dishes for gold-toning and nothing else. This is not very practical if we do many different methods and do not have a cupboard big enough for all jars that might carry catalytic contaminants. Some people use Farmer's reducer (a solution containing potassium ferricyanide and sodium thiosulphate). This is an altogether gentler liquid than nitric acid and it can dissolve silver from black & white photographs. The action of Farmer's reducer on visibly discoloured glass is very slow, and this mixture is not going to destroy insoluble organic forms of dirt such as bits of tissue. Bottom line: Nitric acid, followed by pure (not tap) water. John Kiernan London, Canada. ________________________________ "Scott, Allison D" wrote: > > Hello to all in histoland. I need help in locating a cleaning acid solution > for cleaning glassware. We are having a problem with our GMS stain. I > think it has something to do with the glassware. Thanks in advance > Allison Scott ------------------------- ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 17, Issue 37 **************************************** DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. Morecambe Bay Hospitals NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From eca9 <@t> georgetown.edu Wed Apr 27 11:56:48 2005 From: eca9 <@t> georgetown.edu (Eva C Anderson) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] grade difference? Message-ID: <426FC450.4070700@georgetown.edu> Hi Everone, This rookie would like to know what the difference is between ACS grade and PA grade. Thanks, Eva From julien_lambreydesouza <@t> uqar.qc.ca Wed Apr 27 14:14:09 2005 From: julien_lambreydesouza <@t> uqar.qc.ca (Julien LambreyDeSouza) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Prenan Trichrome Message-ID: <6.1.1.1.1.20050427151001.019b8498@pop3.uqar.qc.ca> Hello all histoneters, Could anybody give insight on the Prenan Trichrome (probably a progressive variation). Does this ring a bell to anyone? I checked on the web and in my all-knowing Humason's animal histology techniques, but I can't find any info on this. Thanks for the help. Julien Lambrey de Souza Assistant de recherche, Biologiste M.Sc. Biologie ?volutive. Universit? du Qu?bec ? Rimouski D?partement de Biologie, Chimie et Sciences sant? 300, all?e des Ursulines Rimouski (Qu?bec) Canada G5L 3A1 T?l.: (418) 723-1986 ext. 1714 Fax.: (418) 724-1849 Courriel: julien_lambreydesouza@uqar.qc.ca From ekh9535 <@t> bjc.org Wed Apr 27 14:52:00 2005 From: ekh9535 <@t> bjc.org (Erin Herter) Date: Fri Sep 16 15:25:00 2005 Subject: [Histonet] Leica ASP 300 Message-ID: Hi Histonetters, A few months ago, I sent an email asking if anyone had a Leica ASP300 and about any problems, etc. (We had been having a lot of problems with ours) A very nice lady from Leica read my email and contacted me. She arranged for someone from Leica to come to our lab and they fixed it!!! We now are VERY happy with our processor and have not had ANY problems since. So thank you to Leica, North Cental Instruments and the Histonet....... Erin Herter, Histology Department Alton Memorial Hospital One Memorial Drive Alton, IL 62002 618-463-7454 618-463-7641 fax ekh9535@bjc.org From madbaza <@t> powerup.com.au Wed Apr 27 17:20:13 2005 From: madbaza <@t> powerup.com.au (Barry Madigan) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] All, In-Reply-To: Message-ID: <20050427222014.RWSV1100.smta09.mail.ozemail.net@WORKSTATION1> Hello Pat I find the major limiting factor in performing Immunostains is the cutting of the sections. We have two Bond X Immunostainers that can handle a total of 30 slides each. These Immunostainers provide us with the flexibility of performing a run of up to 10 slides without tying up the whole machine, thus allowing further slides to be added. This is an enormous bonus when Pathologist like to have their request done urgently. We have had the Bond X Immunostainers for about 18 months now and they have made a major difference in our work flow in Immunohistochemistry. We do not have any cut off point for request and have two staff working in this area. One works from 8am to 4 pm and the other starts at 10am and finishes at 6pm. Before we purchased these stainers we had a cut off point of 10am. Pathologists receive their immunos the same day as requested or at least early the next morning for those request that are received late in the afternoon. For these late requests an overnight run is started just before 6pm using these immunostainers. The biggest impact we made to our workload a number of years ago was to place control material on the same slide as the test. This led to a reduction by at least a third in the total number of immuno slides. Apart from the cutting aspect the only other limiting factor is that our Pathologist occasionally batch their immuno requests or place them in the Laboratory when leaving for the day. This problem is being addressed by introducing an electronic ordering system. Regards Barry Madigan Immunohistochemistry QHPS-RBH campus Brisbane Queensland Australia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Karlisch Sent: Friday, 22 April 2005 12:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] All, All, We are interested in knowing how some of you handle the volume of Immunhistochemistries in your department if you are limited by staff and autostainers. Do you have cut off times for ordering; do you prioritize the work or do you have a turn around time that must be met and what is this time from ordering to finished slides. We have two autostainers that can only handle 20 slides each and 10-15% of the volume is performed manually. One tech of two rotates into this lab. I appreciate any help you can give me. Thank you, Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Secholsb <@t> aol.com Wed Apr 27 19:06:41 2005 From: Secholsb <@t> aol.com (Secholsb@aol.com) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] ROTOAVIRUS Message-ID: <203.76a7eb.2fa18311@aol.com> Does anybody know of a commercial source of antibodies against chicken rotoavirus? Sara Brown From c.m.vanderloos <@t> amc.uva.nl Thu Apr 28 02:09:35 2005 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] RE: need some help with frozen sections-tap vs. distilled water Message-ID: <57cf8a581ca2.581ca257cf8a@amc.uva.nl> Hi Andrea and Gayle, The story of the ruined cryo's after distilled water came from my preparations with the NSH hands-on workshop in Long Beach 2002. I was told that the NSH would supply me with just distilled water for everything. So I rehearsed the experiments here with distilled water only. Next, I was confronted with the poorest IHC staining ever, especially with respect to the nuclear counterstain/tissue morphology. It took me days before I figured out that the final washing step with distilled water was the real problem. I have the strong impression that especially lymphoid tissues are far more vulnerable to "distilled water washing" than other tissues. I don't know if this has something to do with the quality of our distilled water. Perhaps I need to repeat those simple experiments with the more standard MilliQ water as Gayle used. I have placed a picture at [1]www.histonet.org illustrating the difference I found with tap water vs. distilled water (tap vs. dist. water.pdf). Chris van der Loos, PhD Dept. of Pathology Academical Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 fax: +31 20 6960389 e-mail: [2]c.m.vanderloos@amc.uva.nl ----- Original Message ----- From "Andrea T. Hooper" Date Tue, 26 Apr 2005 13:11:27 -0400 To "C.M. van der Loos" , histonet@lists.utsouthwestern.edu Subject [Histonet] RE: need some help with frozen sections Dear Chris, This is an *excellent* summary of what to do and not to do for frozen section IHC! One thing though, you said: "At the end of the IHC staining procedure, after the chromogen step wash your slides with tap water. NEVER use distilled water as this will ruin the tissue section completely!!!!". Why do you think this is so? In my experience, I never use tap water after staining - only MilliQ ddH20 - and have no deleterious effects on my frozen sections of varying thicknesses of both mouse and human tissue - both fixed with NBF and acetone. Best regards, Andrea References 1. http://www.histonet.org/ 2. mailto:c.m.vanderloos@amc.uva.nl From Megan.Clarke <@t> hnehealth.nsw.gov.au Thu Apr 28 02:29:07 2005 From: Megan.Clarke <@t> hnehealth.nsw.gov.au (Megan Clarke) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] searching for these antibodies Message-ID: Hi histonetters Anyone using these antibodies. I would like to purchase these antibodies for use on human FFPET but not sure who the agents are that may supply them. Anti-thyroid peroxidase - clone TPO 47 - The antibody is made by Biocytex, Maiseilles, France. Uroplakin - ?? SV40 - clone Pab 416 - or Pab 101 - oncogene research products ?? supplier Thanks in advance. Zenobia Haffajee HAPS IHC dept Newcastle Australia. From jorge.tornero <@t> gmail.com Thu Apr 28 07:27:46 2005 From: jorge.tornero <@t> gmail.com (Jorge Tornero) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Recycling Technovit 7100 Message-ID: <8c964a790504280527384ce43c@mail.gmail.com> Hello, does anybody have experience about re-using technovit 7100 solutions? For instance, preparing the 1:1 absolute alcohol-resin solution with used preinfiltration solution. Cheers Jorge Tornero IEO-C?diz (Spain) From Sue.Kapoor <@t> uhsi.org Thu Apr 28 07:50:11 2005 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Recycling alcohol / microwave processor Message-ID: <61E9F2400F53D5119CFC00508B44E33B02E9E0F8@khmcexch.uhsi.org> Hi all, I'm interested in hearing if others using microwave tissue processors recycle the alcohol they used in it and/or reuse the alcohol?? Thanks in advance, Sue Kapoor, HT (ASCP) Histology Coordinator Kenosha Medical Center Kenosha, WI 262-653-5570 From ajennings <@t> unmc.edu Thu Apr 28 09:06:10 2005 From: ajennings <@t> unmc.edu (Anita Jennings) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] searching for these antibodies SV40 In-Reply-To: Message-ID: Ahhh this is another one of those....who bought out my supplier.... Calbiochem aka EMD seems to have a lot of the antibodies people are looking for these days....http://www.emdbiosciences.com/product/DP02 same product number as oncogenes....coincidence??? I think...not Anita I use this SV40 at 1:25 on frozen samples viewed with TX red "Megan Clarke" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] searching for these antibodies 04/28/2005 02:29 AM Hi histonetters Anyone using these antibodies. I would like to purchase these antibodies for use on human FFPET but not sure who the agents are that may supply them. Anti-thyroid peroxidase - clone TPO 47 - The antibody is made by Biocytex, Maiseilles, France. Uroplakin - ?? SV40 - clone Pab 416 - or Pab 101 - oncogene research products ?? supplier Thanks in advance. Zenobia Haffajee HAPS IHC dept Newcastle Australia. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andrew.gray <@t> lycos.com Thu Apr 28 10:07:27 2005 From: andrew.gray <@t> lycos.com (Andrew Gray) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Kappa opioid receptor Ab epitope Message-ID: <20050428150727.00D7830E7DF@ws7-1a.us4.outblaze.com> Dear Histonet, I am trying to find out the epitope sequence of the primary antibody targeting kappa opioid receptors, marketed by Sigma (Cat. O1757). Their website and data sheets only describe the epitope as 'internal region'. The literature articles referenced in the data sheet do not comment on this specific antibody. When I e-mailed the company they wouldn't tell me what the epitope sequence was (it probably required more effort than checking the website), and they also refused to identify the manufacturer. Information about any prior testing would also be appreciated. I have been using this antibody and I feel that this lack of information might reduce the publishability of my work. Link: http://www.sigmaaldrich.com/cgi-bin/hsrun/Suite7/Suite/Suite.hjx;start=Suite.HsViewHierarchy.run?Detail=Product&ProductNumber=SIGMA-O1757&VersionSequence=1 Thank you, Andrew Gray PhD student From TMcNemar <@t> lmhealth.org Thu Apr 28 10:06:18 2005 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] TAT for frozens? Message-ID: <6CD94D97ED7D924BA5C2B588FA9528213967B5@mail.lmhealth.org> Hello all, I was wondering what folks are using as a benchmark for frozen section TAT. Do you even have one? We have always listed a 15 minute TAT for but we are not always meeting it these days. The 15 minutes is for single frozens. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio 43055 From Diane.Gladney <@t> se.amedd.army.mil Thu Apr 28 10:32:36 2005 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] TAT for frozens? Message-ID: <4D55B2E997EFAE4DA6081DDE100B83024B1F2C@amedmlsermc133.amed.ds.army.mil> We use a 20 minute TAT for a single. We don't always meet this TAT for various reasons....difficulty in sectioning, unusual case for pathologist to diagnose (needing more time to look at reference books). Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology/Cytology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart Street P.O. Box 484 Ft. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, April 28, 2005 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TAT for frozens? Hello all, I was wondering what folks are using as a benchmark for frozen section TAT. Do you even have one? We have always listed a 15 minute TAT for but we are not always meeting it these days. The 15 minutes is for single frozens. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio 43055 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Thu Apr 28 10:35:06 2005 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] TAT for frozens? Message-ID: <83AACDB0810528418AA106F9AE9B7F7EA45B29@sjhaexc02.sjha.org> That is the CAP requirement. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Thursday, April 28, 2005 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TAT for frozens? Hello all, I was wondering what folks are using as a benchmark for frozen section TAT. Do you even have one? We have always listed a 15 minute TAT for but we are not always meeting it these days. The 15 minutes is for single frozens. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio 43055 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From bill501 <@t> mindspring.com Thu Apr 28 10:38:30 2005 From: bill501 <@t> mindspring.com (Bill) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] TAT for frozens? In-Reply-To: <6CD94D97ED7D924BA5C2B588FA9528213967B5@mail.lmhealth.org> References: <6CD94D97ED7D924BA5C2B588FA9528213967B5@mail.lmhealth.org> Message-ID: At 11:06 AM -0400 4/28/05, Tom McNemar wrote: >I was wondering what folks are using as a benchmark for frozen section TAT. >Do you even have one? We have always listed a 15 minute TAT for but we are >not always meeting it these days. The 15 minutes is for single frozens. IMO, this is a useless benchmark as every case is different. A fozen should takes as long as it needs to take to come up with an appropriate and accurate diagnosis. -- ______________ Bill Blank, MD Heartland Lab From bwhitaker <@t> brownpathology.com Thu Apr 28 10:47:36 2005 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] TAT for frozens? In-Reply-To: <83AACDB0810528418AA106F9AE9B7F7EA45B29@sjhaexc02.sjha.org> Message-ID: <000001c54c09$99c5a250$3601a8c0@brownpathology.net> Technically, it is a cap requirement that it be documented as to why the TAT was greater than 20 minutes. Nothing states that the TAT can't exceed 20 minutes. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, April 28, 2005 10:35 AM To: Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] TAT for frozens? That is the CAP requirement. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Thursday, April 28, 2005 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TAT for frozens? Hello all, I was wondering what folks are using as a benchmark for frozen section TAT. Do you even have one? We have always listed a 15 minute TAT for but we are not always meeting it these days. The 15 minutes is for single frozens. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio 43055 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. From BlazekL <@t> childrensdayton.org Thu Apr 28 11:07:08 2005 From: BlazekL <@t> childrensdayton.org (Linda Blazek) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] TAT for frozens? Message-ID: Thought this might help.Linda ANP.11820 Phase I N/A YES NO Does the laboratory periodically evaluate turnaround time for intraoperative frozen sections? NOTE: If 90% of frozen sections are not completed within 20 minutes, the laboratory must document evaluation of the reason(s) for the delay. This turnaround time is intended to apply to the typical single frozen section. In cases where there are multiple sequential frozen sections required on a single specimen (e.g., resection margins), or in cases where additional studies such as radiographic correlation are required, longer turnaround times may be expected. COMMENTARY: At least 90% of frozen section interpretations should be rendered within 20 minutes of specimen arrival in the frozen section area. This 20-minute turnaround time is intended to apply to the typical single frozen section. If 90% of frozen sections are not completed within 20 minutes, there must be documentation of the reason(s) for the delay. In cases where there are multiple sequential frozen sections required on a single specimen (e.g., resection margins), or in cases where additional studies such as radiographic correlation are required, longer turnaround times may be expected. REFERENCE: Novis DA, Zarbo RJ. Interinstitutional comparison of frozen section turnaround time. A College of American Pathologists Q-Probes study of 32 868 frozen sections in 700 hospitals. Arch Pathol Lab Med. 1997;121:559-567. From failm <@t> musc.edu Thu Apr 28 11:35:36 2005 From: failm <@t> musc.edu (Mildred Fail) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] unknown Black pigment puzzle Message-ID: Hello all, We have a skin punch biopsy containing a black pigment we are trying to identify. Negative for Fe Negative for Copper No change with Ammonium Sulfide Remains after melanin bleach Remains after treatment with concentrated Sulfuric acid Remains after treatment with potassium ferricyanide/hypo solution Is removed by treatment in Lugol's Iodine, followed by hypo, but after this treatment what appears to be crystals are left in several areas in a linear pattern. These do not appear to be Iodine crystals. The patient did have acupuncture treatment, is it possible this is silver pigment? From the needle? Something else as simple as a splinter? Any help would be greatly appreciated Thank you Rena Fail From contact <@t> excaliburpathology.com Thu Apr 28 12:08:13 2005 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] unknown black pigment Message-ID: <20050428170813.51454.qmail@web50308.mail.yahoo.com> Did the patient have a tattoo? Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From Barbara_Lentz <@t> dahlchase.com Thu Apr 28 12:21:36 2005 From: Barbara_Lentz <@t> dahlchase.com (Barbara Lentz) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] unknown black pigment Message-ID: Or had the patient stuck him/herself with a pencil? >>> Paula Pierce 04/28/05 01:08PM >>> Did the patient have a tattoo? Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Thu Apr 28 12:24:22 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] TAT for frozens? Message-ID: Linda, 20 minutes for just one single frozen slide? That seems to be a long time. 10 minutes max. Robyn OHSU From BlazekL <@t> childrensdayton.org Thu Apr 28 12:28:50 2005 From: BlazekL <@t> childrensdayton.org (Linda Blazek) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] TAT for frozens? Message-ID: Robyn, Those were not standards I set. That was a direct reference from the CAP survey. Linda >>> "Robyn Vazquez" 04/28/2005 1:24 PM >>> Linda, 20 minutes for just one single frozen slide? That seems to be a long time. 10 minutes max. Robyn OHSU From vazquezr <@t> ohsu.edu Thu Apr 28 12:35:02 2005 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] TAT for frozens? Message-ID: Linda, So, 20 minutes is a set standard established by CAP's? Thanks, Robyn OHSU From Luis.Chiriboga <@t> med.nyu.edu Thu Apr 28 12:59:26 2005 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Kappa opioid receptor Ab epitope In-Reply-To: <20050428150727.00D7830E7DF@ws7-1a.us4.outblaze.com> Message-ID: I would recommend doing a search in pubmed (look for articles that specifically use the sigma antibody or try and find the article in which the antibody was first characterized) and see what turns up. You should also search different antibody manufacturers. If sigma is the only one who supplies the antibody, then perhaps there is a proprietary reason that they will not relinquish the information. either way, I find it unusual that Sigma would not be forthcoming especially if you mention that you require the info for publication. Hope this helps Luis ____________________________________ Luis Chiriboga Ph.D. NYU Cancer Institute and Bellevue Hospital Center New York University School Of Medicine Department Of Pathology 4W27 462 First Avenue New York, N.Y. 10016 W(212) 562-4667. F(212) 263-2041 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Andrew Gray Sent: Thursday, April 28, 2005 11:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa opioid receptor Ab epitope Dear Histonet, I am trying to find out the epitope sequence of the primary antibody targeting kappa opioid receptors, marketed by Sigma (Cat. O1757). Their website and data sheets only describe the epitope as 'internal region'. The literature articles referenced in the data sheet do not comment on this specific antibody. When I e-mailed the company they wouldn't tell me what the epitope sequence was (it probably required more effort than checking the website), and they also refused to identify the manufacturer. Information about any prior testing would also be appreciated. I have been using this antibody and I feel that this lack of information might reduce the publishability of my work. Link: http://www.sigmaaldrich.com/cgi-bin/hsrun/Suite7/Suite/Suite.hjx;start=Suite .HsViewHierarchy.run?Detail=Product&ProductNumber=SIGMA-O1757&VersionSequenc e=1 Thank you, Andrew Gray PhD student _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MElliott <@t> mrl.ubc.ca Thu Apr 28 12:57:11 2005 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Region IX Education Day Message-ID: I would like to invite everyone to attend the upcoming Region IX "Education Day" being held in beautiful Vancouver British Columbia on Sat. June 25. We will be having 4 speakers talking on a variety of topics, including: basic histology, including fixation, by Bryan Hewlett; Ethel Macrea will be discussing pratice and pitfalls of IHC; Allan Rempel will be discussing basic molecular biiology and ISH; and finally Dr. Steve Kussick will be discussing the role of ISH/FISH/CISH and IHC and their applications to lymphoma diagnosis. In addition, there will be a number of Vendors displaying a variety of items. Lunch will be provided. The event is taking place in the Conference Centre of St. Paul's Hospital, in downtown Vancouver. For more information and registration forms please go to the Region IX Website http://www.nshregionix.org/flyereducday.pdf Come join us for a stimulating day. Mark Elliott Education Chair, Region IX From bsylinda <@t> aol.com Thu Apr 28 13:15:39 2005 From: bsylinda <@t> aol.com (bsylinda@aol.com) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] EGFR IHC Message-ID: <8C71A356AF0ED02-5D4-1A02A@mblk-r38.sysops.aol.com> Hello, We have just started using a new antibody, purchased from biogenix EGFR, and we not having good staining results. Could someone/anyone give some pointers to getting this antibody to work. Antibody sheet suggets that we use pepsin for pretreatment, 2 hr incubation of antibody. I am using e30 clone. Thanks in advance, Sylinda Battle, HT (ASCP) 903-232-3722 903-232-3839 fax From dholmes <@t> anatomy.umsmed.edu Thu Apr 28 13:27:53 2005 From: dholmes <@t> anatomy.umsmed.edu (Dianne Holmes) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] need markers . . . Message-ID: Do any of you work in research? I need some ideas on demonstrating intimal hyperplasia. At present, we are using H&E and also measuring intima/media ratios. Is there some other markers I could use before I go blind reading these micrographs?!!!! From petepath <@t> yahoo.com Thu Apr 28 13:39:54 2005 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Histonet] TAT for frozens? Message-ID: <20050428183954.34836.qmail@web30410.mail.mud.yahoo.com> Pathologist point of view. I think most of us can easily, embed, freeze cut and stain a single slide of a small simple piece of tissue some where between 5 and 10 minutes. Each additional block will add another few minutes. The real time consumer for the pathologist comes in grossing the tissue and reading the slide. Given a large specimen that requires careful inking, detailed dissection, meticulous slicing, and thorough gross examination of each piece the time can add up quickly. In reading the micro, many cases can be obvious in a glance, however some take meticulous screening to find minute clues. Wading through books and asking for intradepartmental consultation will also consume time. I have no problem with following turn around times in order to identify weak pathologists or techs in a department who may benifit from education. It may also help point out that it is time to modernize equiptment and may be a nice piece of data to show your administrators when you are looking for that new cryostat or additional staff. The point I would like to make is that perfoming a thorough gross and a careful micro are extremely important and should not be rushed. A careful gross is our best defense against sampling error. A careful micro will avoid any embarrasing surprises on permenents. Whether your a tech preparing the slides or a pathologist grossing and reading them it is very important that we avoid letting anyone make us feel rushed. None of us can do our best under pressure and I urge anyone in this business to make believe you are working on the tissue of a family member. The patients surgery is costing an a great deal and often totally hinges on what we say on frozen. An if we are wrong that same surgeon who rushed us will be puting the blame on us the next day when we have given a wrong diagnosis or find out we really don't have enough tissue for the special studies the patient went to surgery for. My suggestion: Worry about TAT stats for the simple ones and do them all as carefully as you can. If it is complicated it will take more time. These can be documented as comlicated for TAT records. When a surgeon calls to speed me up me in the middle of a case I like to tell them " Now you made me nervous and I have to go to the bathroom. It will take another 10 minutes" Stephen From tdobersztyn <@t> chmca.org Thu Apr 28 13:43:36 2005 From: tdobersztyn <@t> chmca.org (tdobersztyn@chmca.org) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] HT/EM senior tech position OHIO Message-ID: Hi fellow Histonetters, I would like to convey that correspondence for this HT position should be done by interested fellow technicians ONLY. Please do not call if you are a recruiter/recruiting company. All interest in this position can be directed to myself or the CHMCA.org web site- careers available: Allied Heath link A flux of calls from recruitment companies has become somewhat overwhelming. but thank you for understanding. Theresa R Dobersztyn HT ASCP Electron Microscopy Laboratory Department of Pathology Akron Children's Hospital 1 Perkins Square Akron, Ohio 44308 330-543-8279 From jstaruk <@t> masshistology.com Thu Apr 28 14:53:14 2005 From: jstaruk <@t> masshistology.com (Jim Staruk) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] need markers . . . In-Reply-To: Message-ID: <0IFO006YV9WMZFV6@vms048.mailsrvcs.net> We use an elastic stain to differentiate intima from the rest of the arterial wall. Check out a beautiful demonstration of intimal hyperplasia using an elastic stain on our welcoming homepage. Jim ______________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dianne Holmes Sent: Thursday, April 28, 2005 2:28 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] need markers . . . Do any of you work in research? I need some ideas on demonstrating intimal hyperplasia. At present, we are using H&E and also measuring intima/media ratios. Is there some other markers I could use before I go blind reading these micrographs?!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TillRenee <@t> uams.edu Thu Apr 28 15:29:19 2005 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Pierce DAB Message-ID: Is there some secret to this I haven't figured out? I ordered the Pierce Metal Enhanced DAB, thinking I might like the brownish/black better, and it's supposedly more sensitive. I've now tried it twice and ended up ruining two whole days work because it did absolutely nothing. No brown, no black. I've read and reread the mixing instructions and I can't see where I could've went wrong. I dug some regular DAB out of a kit and it won't even work after the other has been on the slides. Any ideas? These are identical immuno runs that worked perfectly before I switched DAB. Renee' Till, HT Arkansas Childrens Nutrition Center 1120 Marshall Little Rock, AR 72202 (501) 364-2774 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From drsvsrini <@t> yahoo.com Thu Apr 28 16:34:11 2005 From: drsvsrini <@t> yahoo.com (Mr srinivas sv) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] i need some technical help! Message-ID: <20050428213411.99581.qmail@web31506.mail.mud.yahoo.com> Hi, Does anyone have a protocol or reference for cleaning up polyclonals with acetone-extracted liver powder? I am trying to look at the expression of steroidogenic enzymes in the sheep ovary. My sections are cryosections. All my antibodies are polyclonal antibodies. My problem is that there is color development in both positive and negative controls. I am using normal rabbit sera (from SIGMA) for the negative control as my primary antibodies are raised in rabbit. I am using Normal goat sera and 1% BSA for blocking nonspecific antigens (Serum Blocking) as my secondary antibody is raised in Goat. I am also using 1% hydrogen peroxide for blocking endogenous peroxides. Fed up with this, i now want to try using acetone-extracted liver powder as an adsorbent. Can anyone explain me the use of acetone-extracted liver powder and protocol for using it for my experiment. I am trying this from past 6 months, without any positive result. So, anyone please troubleshoot my problem and suggest any alteration in my protocol. Please feel free to ask for any clarifications. Thanks... Regards, Srinivas Srinivas Seekallu PhD student Dept of Vet Biomed.Sci. Western College of Veterinary Medicine, University of Saskatchewan. Saskatoon, Canada. Ph: 306-477-0849 (Home) 306-966-7373 (Lab) 306-966-7382 (Office) __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Abizar.A.Lakdawalla <@t> appliedbiosystems.com Thu Apr 28 16:43:41 2005 From: Abizar.A.Lakdawalla <@t> appliedbiosystems.com (Abizar A Lakdawalla) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] i need some technical help! In-Reply-To: <20050428213411.99581.qmail@web31506.mail.mud.yahoo.com> Message-ID: I am trying to look at the expression of steroidogenic enzymes in the sheep ovary. My sections are cryosections. All my antibodies are polyclonal antibodies. ARE YOUR ANTIBODIES SHEEP OR GOAT POLYCLONALS? AND ARE YOU USING AN ANTI-SHEEP/GOAT SECONDARY ANTIBODY? ABIZAR From KMB1904 <@t> aol.com Thu Apr 28 16:44:30 2005 From: KMB1904 <@t> aol.com (KMB1904@aol.com) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Histology position ...Delaware Message-ID: <104.601d47b6.2fa2b33e@aol.com> Histology position ...ASCP certified or eligible tech wanted. FT tech needed for a busy hospital lab. The hours are 8:30 to 5pm M-F. Seaford, De is close to the beach and the cost of living is low! Come check us out! Please contact Nanticoke Memorial Hospital Human Resources Department. 302-629-6611 From mcauliff <@t> umdnj.edu Thu Apr 28 16:33:59 2005 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Pierce DAB In-Reply-To: References: Message-ID: <427156C7.60704@umdnj.edu> 1. Is your peroxide fresh? It turns to water over time. 2. Call Pierce and see if they have any ideas. Geoff Till, Renee wrote: >Is there some secret to this I haven't figured out? I ordered the Pierce >Metal Enhanced DAB, thinking I might like the brownish/black better, and >it's supposedly more sensitive. I've now tried it twice and ended up >ruining two whole days work because it did absolutely nothing. No brown, >no black. I've read and reread the mixing instructions and I can't see >where I could've went wrong. I dug some regular DAB out of a kit and it >won't even work after the other has been on the slides. Any ideas? These >are identical immuno runs that worked perfectly before I switched DAB. > > > >Renee' Till, HT > > > >Arkansas Childrens Nutrition Center > >1120 Marshall > >Little Rock, AR > >72202 > > > >(501) 364-2774 > > > > >Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From danaspears <@t> frontiernet.net Thu Apr 28 17:20:06 2005 From: danaspears <@t> frontiernet.net (Dana Spears) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Looking for Sharon Lang Message-ID: <010301c54c40$6e9d2ee0$02fea8c0@VENTANAB4561C6> If anyone has Sharon's contact info, could you please forward it onto me? Thanks! I think she is at BioGenex now? From danaspears <@t> frontiernet.net Thu Apr 28 17:21:55 2005 From: danaspears <@t> frontiernet.net (Dana Spears) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] 2005 Illinois Society for Histotechnologists Meeting Message-ID: <011001c54c40$af6559c0$02fea8c0@VENTANAB4561C6> Histonetters, Just wanted to remind you that the Illinois Meeting is rapidly approaching! It will be held May 12 & 13 at the Mark Twain Hotel in Peoria, IL. We have some terrific speakers this year - please check out the program on our website - www.ilhisto.org Hope to see you there! Dana Spears (309) 255-7214 From claus <@t> itsa.ucsf.edu Thu Apr 28 17:45:04 2005 From: claus <@t> itsa.ucsf.edu (Claudia Schaller) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] beta-Galactosidase Antibody Message-ID: <42716770.2050309@itsa.ucsf.edu> Looking for the best antibody!! Someone out there who has tried to detect Beta-Galactosidase (lacZ reporter) on Bouin's fixed and paraffin embedded mouse testis? Did someone try the polyclonal from Novus Biologicals? Any good experiences with other companies? I'm also happy to get some information about staining in other tissues! Any inormation will be helpful! Thanks! Claudia Schaller/UCSF From jorge.tornero <@t> gmail.com Fri Apr 29 02:31:14 2005 From: jorge.tornero <@t> gmail.com (Jorge Tornero) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Recycling Technovit 7100 In-Reply-To: <042820051316.27294.4270E2400004783D00006A9E2205886014CECE030E9D0C9A03@comcast.net> References: <042820051316.27294.4270E2400004783D00006A9E2205886014CECE030E9D0C9A03@comcast.net> Message-ID: <8c964a7905042900313d16bc6b@mail.gmail.com> I'll try to reuse the infiltration solution (pure resin) to make the 1:1 solution. I'll tell you the results if I can Anyway, thank you, Pam 2005/4/28, mucram11@comcast.net : > > Hi Jorge, > > I have reused my dilutions of GMA (7100 and JB-4 or JB-4 Plus) for several > infiltrations. I just watch the material and if I see or think I am adding > to much alcohol to the mix I have added more resin. I don't use it for more > than a 6 changes as I find it will sometimes change viscosity and I don't > want it too thick. Some people feel this wrong and will not reuse so I > think if is really personal experince and being careful to not over use the > solutions. > > Pam Marcum > > -------------- Original message -------------- > > > Hello, > > > > does anybody have experience about re-using technovit 7100 solutions? > > > > For instance, preparing the 1:1 absolute alcohol-resin solution with > > used preinfiltration solution. > > > > Cheers > > > > Jorge Tornero > > IEO-C?diz (Spain) > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri Apr 29 05:15:23 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] HT scores Message-ID: <008a01c54ca4$5df4d0c0$8b25d445@domainnotset.invalid> For those interested in the HT and HTL pass rates for July -Dec 2004. Taken from the ASCP BOR webpage. MCQ = Multiple Choice Questions (i.e., written/computer) PRACT = Practical Minimum Pass Score required = 400 Range of scores theoretically possible = 100-999 NAACLS = National Accrediting Agency for Clinical Laboratory Sciences (i.e., accredited HT programs) MCQ - NAACLS & non-NAACLS - Mean = 373 - Range = 100-761 - Total # taking exam = 1213 - Total # pass = 478 (39%) - NAACLS students - # taking exam = 148 - # pass = 111 (75%) - non-NAACLS candidates - # taking exam = 1065 - # pass = 367 (34.5%) PRACT (H&E - artery, kidney, skin, tonsil, cervix; Trichrome - liver; Prussian blue - spleen; Mucicarmine - small intestine; GMS - fungus) - NAACLS & non-NAACLS - Mean = 474 - Range = 100-865 - Total # taking exam = 1101 - Total # pass = 833 (76%) - NAACLS students - # taking exam = 149 - # pass = 126 (87%) - non-NAACLS candidates - # taking exam = 952 - # pass = 707 (74%) COMBINED MCQ & PRACT - NAACLS & non-NAACLS - Total # taking exam = 429 - Total # pass = 1176 (36.5%) - NAACLS students - # taking exam = 97 - # pass = 139 (70%) - non-NAACLS candidates - # taking exam = 332 - # pass = 1037 (31%) HT Exam first given in 1948. Number certified to date = 19,515 Comments from me, from observations of watching these numbers for decades: The usual pass rates are: - NAACLS students = 65-75%, so students in this session had the usual pass rate - non-NAACLS candidates = 40-45%, so candidates in this session did worse than usual. However, I have seen this phenomenon before, when there have been other "deadlines" for people working in the histology field to take a certification exam (or their perception that it might be required), for example during the beginnings of DRG's (mid-1980's) and just before CLIA '88 was enacted (early 1990's). There is a lower pass rate. Probably many reasons why - definitely not prepared for the caliber of the exam, possibly just wanting to get the exam in before the deadline and planned on studying harder for the next time they took it after the deadline, or a myriad of other reasons. And, no, it was not a harder written exam. Many of the questions in the pool are the same for the past 10-20 or more years. The theory on a GMS or PAS has been the same for a century. Yes, there are newer questions (microwaves, recycling, safety, for example), but these are tested and then referenced to already existing questions that have a long history. So their degree of difficulty is referenced to other questions of the same degree of difficulty. Anyway, thought people might be interested. I'll post the HTL scores in the next email. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From lpwenk <@t> sbcglobal.net Fri Apr 29 05:27:26 2005 From: lpwenk <@t> sbcglobal.net (lpwenk@sbcglobal.net) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] HTL exam scores Message-ID: <009001c54ca6$0c196480$8b25d445@domainnotset.invalid> For those interested in the HTL pass rates for July -Dec 2004. Taken from the ASCP BOR webpage. MCQ = Multiple Choice Questions (i.e., written/computer) PRACT = Practical Minimum Pass Score required = 400 Range of scores theoretically possible = 100-999 No scores listed for NAACLS students = National Accrediting Agency for Clinical Laboratory Sciences (i.e., accredited HT programs) (I've been told that's because the numbers are too low to be statistically accurate to report.) MCQ - NAACLS & non-NAACLS - Mean = 437 - Range = 100-656 - Total # taking exam = 110 - Total # pass = 73 (66%) PRACT (H&E - cerebellum, ovary, thyroid; Trichrome - uterus; Verhoeff van Gieson - artery; Mucicarmine - small intestine; Colloidal iron - lung, Silver stain - Helicobacter pylorii; IHC Chromagranin - pancreas) - NAACLS & non-NAACLS - Mean = 478 - Range = 100-678 - Total # taking exam = 83 - Total # pass = 69 (83%) COMBINED MCQ & PRACT - NAACLS & non-NAACLS - Total # taking exam = 104 - Total # pass = 64 (61.5%) Some comments from me, from observations of past tests: - Usual HTL pass rate = 45-60%, so this cycle is typical. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From c.m.vanderloos <@t> amc.uva.nl Fri Apr 29 06:51:25 2005 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] pictures at histonet.org Message-ID: <5c48dc5cb2fe.5cb2fe5c48dc@amc.uva.nl> L.S. Yesterday I said I would put a picture at the "list server images" of the Histonet.org website showing the difference of using tap water vs. distilled water washing. I tried to send those pictures according to the instructions to: [1]pictures@histonet.org. However, any e-mail - with or without a picture - to this address is returning to sender immediately. What's wrong here? Chris van der Loos, PhD Dept. of Pathology Academical Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 fax: +31 20 6960389 e-mail: [2]c.m.vanderloos@amc.uva.nl References 1. mailto:pictures@histonet.org 2. mailto:c.m.vanderloos@amc.uva.nl From pruegg <@t> ihctech.net Fri Apr 29 07:54:22 2005 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] F8 for porcine Message-ID: <200504291254.j3TCsPmh045731@pro12.abac.com> Does anyone know if Dako's rabbit polyclonal anti-human Factor 8 will cross react with pig endothelial cells? Patsy From dellav <@t> musc.edu Fri Apr 29 09:14:36 2005 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Region IX Education Day Message-ID: good luck and best wishes for a successful "education day" wish I could be there. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Mark Elliott" 04/28/05 01:57PM >>> I would like to invite everyone to attend the upcoming Region IX "Education Day" being held in beautiful Vancouver British Columbia on Sat. June 25. We will be having 4 speakers talking on a variety of topics, including: basic histology, including fixation, by Bryan Hewlett; Ethel Macrea will be discussing pratice and pitfalls of IHC; Allan Rempel will be discussing basic molecular biiology and ISH; and finally Dr. Steve Kussick will be discussing the role of ISH/FISH/CISH and IHC and their applications to lymphoma diagnosis. In addition, there will be a number of Vendors displaying a variety of items. Lunch will be provided. The event is taking place in the Conference Centre of St. Paul's Hospital, in downtown Vancouver. For more information and registration forms please go to the Region IX Website http://www.nshregionix.org/flyereducday.pdf Come join us for a stimulating day. Mark Elliott Education Chair, Region IX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histalim <@t> hotmail.fr Fri Apr 29 09:54:11 2005 From: histalim <@t> hotmail.fr (sarl HISTALIM) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] soybean cells Message-ID: Hello Does anyone know a product to collocate specifically soybean cells. Thank you very mutch for your help. Jean-Philippe COTON PS : I'm working at HISTALIM, a food histology lab. In France. We analyze products with meat like sausages, burgers www.histalim.com HISTALIM, l'histologie alimentaire www.histalim.com tel : 06.19.64.67.37. _________________________________________________________________ Ne cherchez plus, trouvez ! [1]Avec le nouveau MSN Search References 1. http://g.msn.com/8HMAFRFR/2749??PS=47575 From dellav <@t> musc.edu Fri Apr 29 10:19:13 2005 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] i need some technical help! Message-ID: I am sharing information taken from Fluorescent Protein Tracing by R.C Nairn, Livingstone Ltd 3rd ed. 1969. This information is also cited in Principles and Techniques in Diagnostic Histopathology, JM Elias, Noyes Publications, 1982 Liver absorption is frequently employed to purify antibodies simply because it is a large celullar organ. liver powder from the species you are studying (in this case Sheep) should be used. You will need to find a source for sheep liver powder. if you cannot locate a source but have access to sheep liver, contact me and I will provide you with the information to prepare your own. for absorption purposes, 100 mg of sheep liver powder is mixed with 1 ml of the original serum used (your primary antibody). the proportions are not critical and rough measurement is quite satisfactory. the mixture is shaken at room temperature for 2 hours at a speed that avoids frothing after which the mixture is centrifuged at 10,000g for 15 minutes and the supernatant is removed there is no harm in doing the absorption however it isn't clear from your message whether this will solve your background staining problem. can we assume that you are achieving the desired specific staining with the primary antibody and simply wish to clean up the background? how did you arrive at the correct dilution of your primary antibody ?? is the primary 'home-grown' or purchased commercially ?? please let us know the outcome of the absorption Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> Mr srinivas sv 04/28/05 05:34PM >>> Hi, Does anyone have a protocol or reference for cleaning up polyclonals with acetone-extracted liver powder? I am trying to look at the expression of steroidogenic enzymes in the sheep ovary. My sections are cryosections. All my antibodies are polyclonal antibodies. My problem is that there is color development in both positive and negative controls. I am using normal rabbit sera (from SIGMA) for the negative control as my primary antibodies are raised in rabbit. I am using Normal goat sera and 1% BSA for blocking nonspecific antigens (Serum Blocking) as my secondary antibody is raised in Goat. I am also using 1% hydrogen peroxide for blocking endogenous peroxides. Fed up with this, i now want to try using acetone-extracted liver powder as an adsorbent. Can anyone explain me the use of acetone-extracted liver powder and protocol for using it for my experiment. I am trying this from past 6 months, without any positive result. So, anyone please troubleshoot my problem and suggest any alteration in my protocol. Please feel free to ask for any clarifications. Thanks... Regards, Srinivas Srinivas Seekallu PhD student Dept of Vet Biomed.Sci. Western College of Veterinary Medicine, University of Saskatchewan. Saskatoon, Canada. Ph: 306-477-0849 (Home) 306-966-7373 (Lab) 306-966-7382 (Office) __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From matt <@t> ategra.com Fri Apr 29 12:16:48 2005 From: matt <@t> ategra.com (Matt Hawes (extension 224)) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] New Histology and MOH's Technologist Job Opportunities April 29th 2005 Message-ID: Hello , My name is Matt Hawes and I am the Senior AP Recruiter here at Ategra Systems and I have received new Histology and MOH's Technologist Job Opportunities that I wanted to inform you about and see if you might be interested or if you might happen to know of anyone looking for new employment opportunities? Here are some of my HOTTEST Fulltime Permanent Histo Tech positions & MOH's Tech opportunities: 1. Atlanta, GA- Histo Tech (All Shifts) 2. Ft.Lauderdale, FL- Histo Tech Days Perm 3. Ft.Lauderdale, FL- Histo Tech Days Temporary (12 weeks) 4. Boston Burbs, MA- MOH's/ Histo Tech- Day Shift 5. Northeastern MI- Histo Tech-Day Shift 6. Sioux City, IA- Histo Tech-Day Shift 7. Los Angeles, CA- IHC/Hist Supervisor 8. Rhode Island- Histo Tech-Day Shift 9. Northern New Jersey-Histo Tech- Nite Shift (3 openings) 10. Northest New Jersey- Histo Tech Day Shift (7 Openings all shifts) 11. Upstate NY- Histo Tech- Day Shift (2 openings) And we also have a Cytogenetics position available in New Jersey as well These positions are as direct employees of our clients. They all offer full employee benefits (if working in a fulltime capacity): excellent healthcare, life insurance, dental, vision, 401K/retirement - as well as relocation bonuses (where applic). If you are interested in these jobs, please CALL ME ASAP at (800)466 9919 x224 or e-mail me at Matt@ategra.com . To speed things up, please also send me a copy of your resume, (if you haven't already done so). If you are interested in jobs outside the above-mentioned areas, please send me your resume as well as I have clients throughout the US. I will keep your resume confidential and will not release it to anyone without your permission (This is Ategra policy as well as my own). My services are at no charge to you. Of course, you may be happy in your present job, but it never hurts to to keep an eye open. Also, if you have friends/peers who might be interested as well, if you could pass my query & name on to them I'd be very grateful. However, if you are interested in any of the jobs above, please call me. Thank You !! Matt Hawes Senior Laboratory Recruiter Ategra Systems (800) 466-9919 Ext 234 Matt@Ategra.com --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing VOICE: 407-671-5800 ext 224 TOLLFREE: 800-466-9919 Ext 224 Note: this message is intended for: at angelofmusic1178@aol.com To Learn More About Ategra: http://www.ategra.com -------------------------------------------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. -------------------------------------------------------------------------------------------------- From lfidgen <@t> vt.edu Fri Apr 29 14:29:25 2005 From: lfidgen <@t> vt.edu (Laura Fidgen) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Decalification and epoxy resin Message-ID: <6.0.0.22.0.20050429152708.02519de8@pop.vt.edu> We have a pathologist beginning a study with rat bones and the question he would like answered is: Are there any decalcification solutions that are NOT miscible with Epoxy resins? Thanks in advance! Laura L. Fidgen, MScF, BSc, HT(ASCP) Laboratory and Research Practioner VMRCVM, Histopatholgy/Clinical Pathology Dept. Blacksburg, VA 24060-0443 From cfavara <@t> niaid.nih.gov Fri Apr 29 17:08:12 2005 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] slide storage Message-ID: Many years ago -like in the 60's-70's I remember seeing a slide storage system that allowed on to leave slides on metal trays and then put them in a cabinet. Does anyone out there know of such a system? The reason I ask is that I have an investigator that wants all the slides in trays until he figures things out. This sometimes takes a year or more. Needless to say my counter space and book shelves are being over run. Please help if you can. Good weekend to all. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives From portera203 <@t> yahoo.com Fri Apr 29 17:48:05 2005 From: portera203 <@t> yahoo.com (Amy Porter) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] F8 for porcine In-Reply-To: 6667 Message-ID: <20050429224805.17295.qmail@web40904.mail.yahoo.com> Patsy - I believe that we have used this antibody on skin samples in the past without any problems. Patsy Ruegg wrote:Does anyone know if Dako's rabbit polyclonal anti-human Factor 8 will cross react with pig endothelial cells? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Amy S.Porter, HT(ASCP), QIHC Michigan State University - Department of Physiology Division of Human Pathology Investigative Histopathology Laboratory 2201 Biomedical Physical Sciences Room 2133 East Lansing, MI 48824-3320 Ph: 517-355-6475 ext 1480/1481 Fax: 517-432-1368 portera@msu.edu __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From leswes <@t> shaw.ca Fri Apr 29 18:23:04 2005 From: leswes <@t> shaw.ca (Lesley Weston) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Decalification and epoxy resin In-Reply-To: <6.0.0.22.0.20050429152708.02519de8@pop.vt.edu> Message-ID: If you mean miscible literally, then none of them are - they're all aqueous. If you mean is it OK to use any or all of them before processing for epoxy, then I'm not sure about the various acids, but I do know that EDTA works; I used it as part of my protocol for many years. -- Lesley Weston 3512 W. 31st. Avenue Vancouver, B.C. Canada, V6S 1X9 604-263-3122 leswes@shaw.ca > From: Laura Fidgen > Date: Fri, 29 Apr 2005 15:29:25 -0400 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Decalification and epoxy resin > > We have a pathologist beginning a study with rat bones and the question he > would like answered is: Are there any decalcification solutions that are > NOT miscible with Epoxy resins? Thanks in advance! > > Laura L. Fidgen, MScF, BSc, HT(ASCP) > Laboratory and Research Practioner > VMRCVM, Histopatholgy/Clinical Pathology Dept. > Blacksburg, VA 24060-0443 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From murtaugh <@t> genetics.utah.edu Fri Apr 29 23:03:18 2005 From: murtaugh <@t> genetics.utah.edu (Charles Murtaugh) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] h&e staining on frozen sections Message-ID: Hi all-- I have a problem that has plagued me for years, and is particularly annoying right now because I am trying to move entirely away from doing wax sections to frozen sections (for better preservation of antigens, ease of processing, etc.). I study mouse developmental biology, and I always like to stain one section from every block I process by standard H&E techniques. On wax sections, these are always nice, but on frozen they are invariably dreadful. For frozen sections, I first fix the tissues 1-4 hrs in 4% PFA/PBS, then treat overnight with 30% sucrose/PBS and embed in OCT. My students and I usually get really nice frozen sections, from 8-12 microns depending on what we want to look at, and typically we analyze by fluorescent microscopy or in situ hybridization. Whenever we do H&E staining, though, we get all this artifactual shrinking of the tissue, presumably due to the subsequent dehydration, as well as very poor differentiation of hematoxylin-stained nuclei. With wax neither of these is a problem, even though the initial fixation conditions are identical. Has anyone else encountered (and hopefully solved) this problem? Thanks in advance, Charles Murtaugh ===== L. Charles Murtaugh University of Utah Dept. of Human Genetics 15 N. 2030 E. Rm. 4420B Salt Lake City, UT 84113 tel 801-581-5958 fax 801-581-7796 email murtaugh@genetics.utah.edu From gu.lang <@t> gmx.at Sat Apr 30 10:39:39 2005 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] giemsa stain, green-blue acid mucin Message-ID: Hallo, Last time we stained a gastric biopsy with Giemsa. We found cells with green-blue acid mucin. I have never seen this before. It looks like the mucins in the movat-stain. Can anyone tell me the theoretical background about this? We do the Giemsa on the Ventana-stainer, with following differentiation in A.d. with a few drops glacial acetic acid. Thank you Gudrun Lang From bhewlett <@t> cogeco.ca Sat Apr 30 11:11:52 2005 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] h&e staining on frozen sections References: Message-ID: <000301c54d9f$528a6eb0$6500a8c0@mainbox> Charles, This is the technique that I have found to be useful. Prepare your tissue as usual. Section on the cryostat and handle the sections for fluorescence, ISH etc as usual. Cut the frozen section to be stained for H&E at 4-6 micrometer thickness, lift the anti-roll plate and pickup the flat section on a room temperature, positively charged slide. Immediately immerse the slide in a coplin jar containing the following fixative; ETOH.... 70.0 mL Formalin (37-40% formaldehyde).... 10.0 mL Glacial acetic acid........5.0 mL D.Water to make up to 100 mL. Allow the section to fix for 2 - 5 minutes Place slide in 70% ETOH x2 for 30 seconds each, followed by two changes D. water. Proceed with the H&E stain. The resulting stained section will be virtually indistinguishable from a FFPE stained section. Regards, Bryan Hewlett ----- Original Message ----- From: "Charles Murtaugh" To: Sent: Saturday, April 30, 2005 12:03 AM Subject: [Histonet] h&e staining on frozen sections Hi all-- I have a problem that has plagued me for years, and is particularly annoying right now because I am trying to move entirely away from doing wax sections to frozen sections (for better preservation of antigens, ease of processing, etc.). I study mouse developmental biology, and I always like to stain one section from every block I process by standard H&E techniques. On wax sections, these are always nice, but on frozen they are invariably dreadful. For frozen sections, I first fix the tissues 1-4 hrs in 4% PFA/PBS, then treat overnight with 30% sucrose/PBS and embed in OCT. My students and I usually get really nice frozen sections, from 8-12 microns depending on what we want to look at, and typically we analyze by fluorescent microscopy or in situ hybridization. Whenever we do H&E staining, though, we get all this artifactual shrinking of the tissue, presumably due to the subsequent dehydration, as well as very poor differentiation of hematoxylin-stained nuclei. With wax neither of these is a problem, even though the initial fixation conditions are identical. Has anyone else encountered (and hopefully solved) this problem? Thanks in advance, Charles Murtaugh ===== L. Charles Murtaugh University of Utah Dept. of Human Genetics 15 N. 2030 E. Rm. 4420B Salt Lake City, UT 84113 tel 801-581-5958 fax 801-581-7796 email murtaugh@genetics.utah.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ernestinemiddleton <@t> yahoo.ca Sat Apr 30 17:15:45 2005 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] fungus control Message-ID: <20050430221546.95814.qmail@web51505.mail.yahoo.com> Hi; The person who wanted fungus control blocks in exchange for AFB+ blocks, please e-mail me directly or call me at 718-920-4157. Ernestine Middleton, Manager, BS/MPA, HT/HTL Dept. Pathology Montefiore Medical Center Bronx, NY --------------------------------- Post your free ad now! Yahoo! Canada Personals From allison_m_ont <@t> hotmail.com Sat Apr 30 20:12:50 2005 From: allison_m_ont <@t> hotmail.com (Allison MacRostie) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Seeking Metro DC Jobs Message-ID: Hello, I am a histotech with six years of experience including immunohistochemistry. I am currently seeking a position within the Washington DC area. If anyone has any information please email me and I can forward a copy of my resume. Thanks for any help! Allison allison_m_ont@hotmail.com _________________________________________________________________ Take advantage of powerful junk e-mail filters built on patented Microsoft® SmartScreen Technology. http://join.msn.com/?pgmarket=en-ca&page=byoa/prem&xAPID=1994&DI=1034&SU=http://hotmail.com/enca&HL=Market_MSNIS_Taglines Start enjoying all the benefits of MSN® Premium right now and get the first two months FREE*. From steven.coakley <@t> mirusbio.com Wed Apr 27 09:12:58 2005 From: steven.coakley <@t> mirusbio.com (Steven Coakley) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] Cold acetone Message-ID: I hope this doesn't sound to lame. 13 years in this field and I've never used "cold acetone". The only ref. I have is to use -20 cold acetone. So how do I get my acetone to -20. Do I place a coplin jar of covered actenone in the cyostat for a period of time?????. \\Steve From drsvsrini <@t> yahoo.com Thu Apr 28 16:04:33 2005 From: drsvsrini <@t> yahoo.com (Mr srinivas sv) Date: Fri Sep 16 15:25:01 2005 Subject: [Histonet] i need some technical help! Message-ID: <20050428210433.71641.qmail@web31503.mail.mud.yahoo.com> Hi, Does anyone have a protocol or reference for cleaning up polyclonals with acetone-extracted liver powder? I am trying to look at the expression of steroidogenic enzymes in the sheep ovary. My sections are cryosections. All my antibodies are polyclonal antibodies. My problem is that there is color development in both positive and negative controls. I am using normal rabbit sera ( from SIGMA) for the negative control as my primary antibodies are raised in rabbit. I am using Normal goat sera and 1% BSA for blocking nonspecific antigens (Serum Blocking) as my secondary antibody is raised in Goat. I am also using 1% hydrogen peroxide for blocking endogenous peroxides. Fed up with this, i now want to try using acetone-extracted liver powder as an adsorbent. Can anyone explain me the use of acetone-extracted liver powder and protocol for using it for my experiment. I am trying this from past 6 months, without any positive result. So, anyone please troubleshoot my problem and suggest any alteration in my protocol. Please feel free to ask for any clarifications. Thanks... Regards, Srinivas Srinivas Seekallu PhD student Dept of Vet Biomed.Sci. Western College of Veterinary Medicine, University of Saskatchewan. Saskatoon, Canada. Ph: 306-477-0849 (Home) 306-966-7373 (Lab) 306-966-7382 (Office) __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com