[Histonet] (no subject)

[Gayle Callis]

In reply to using fluorophore labelled primary antibodies, you can still 
use these for immunohistochemistry if you just come back with a secondary 
antibody that is anti FITC, antiPE, etc.  DAKO has a rabbit antiFITC that 
is excellent, there are others on the markey.  If you want to get away from 
a biotin systems, you can always use a polymer immunostaining system to 
detect the rabbit antiFITC.

It should work very well.  Sorry I can't help you with antibody source.

You wrote:

Collagen IV subtypes: alpha 3-alpha 6,

Does anyone know where I can get antibodies to subtypes of all collagen IV
chains (alpha 1–alpha 6)?

Are they only available for immunofluorescence detection?

SantaCruz has some antibodies for collagen IV alpha 1, alpha 2 and alpha 3
isoforms. I also know that ID Labs in Canada has a clone specific to alpha 1
and alpha 2.




Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)