[Histonet] Any fixative to reduce background in immunofluorescence?

Tan, MinHan MinHan.Tan <@t> vai.org
Wed Oct 27 12:14:29 CDT 2004


Hi,
 
I am preparing to do some immunofluorescence staining in kidney tissue
with cd31.
 
Some preliminary work in formalin fixed paraffin embedded tissue has
yielded quite significant background fluorescence. 
 
A colleague suggested that I use frozen tissue, but the morphology is
quite unacceptable. 
 
Another colleague suggested paraformaldehyde, but that is really
inconvenient (preparing this every morning!?)
 
Any advice on a good commercial fixative for this purpose, that doesn't
destroy vessel antigens?

Thanks.
 
Min-Han

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