[Histonet] Sectionnong whole-body rats with a BRIGHT 8250 Cryostat

[Marie-Helene.Lavergne <@t> mdsps.com]

   Hi everyone,

   I'm  using  a  Bright  8250 cryostat to do rat whole-body sections for
   QWBA  analysis.  Since I started using the microtome, (even with blood
   standards  blocks  or  a rat carcass) sometimes (1 time/10), the knife
   appears  to slip on the block at the begginning of the block and start
   to  section  at  the  end.   I  tried two type of knife (D-profile and
   tungsten) and many angles (generaly 10 degree for the D-profile and 20
   for tungsten)

   I  tried  to  adjust the slide tensioners. I tried two types of CMC. I
   tried different temperature (-20 c, -25 c) and it still doesn't work.



   WHAT IS THE PROBLEM????

   

   Please, HELP ME, I'm trying to solve this problem since many months.



   Marie-Helene Lavergne

   Analyst DMPK

   MDS Pharma

   [1]marie-helene.lavergne <@t> mdsps.com

   

References

   1. file://localhost/tmp/mne@mdsps.com