[Histonet] RE: Cox -2 IHC

[C.M. van der Loos]

Dear Moumita Kundu,
If the original protocol for this COX-2 antibody was set up with a TSA detection kit (and was working) there is a good chance that detection of this antibody with neither  LSAB+ nor LSAB2 will show any specific positive staining. Simply because those detection systems are far less sensitive/efficient. My message is to test both detection systems and compare your results.

Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands

----- Original Message ----- 
>From  "Moumita Kundu" <mkundu <@t> purdue.edu> 
Date  Tue, 11 May 2004 10:56:04 -0500 
To  "Elizabeth Chlipala" <lizchlipala <@t> premierhistology.com>, "'Histonet'" <Histonet <@t> lists.utsouthwestern.edu> 
Subject  [Histonet] Cox -2 IHC 
I am trying to do IHC with Cox -2 ,The protocol I have is a long overnight
incubation with TSA kit.
I am trying to reduce the time of incubation using Dako's LSAB+  system or
LSAB2 .
Please pass me any protocol which can be helpful.

Thank you in advance.