[Histonet] Negative control for immuno slides

[Anissa Choi]

We do our negative reagent control by omitting the primary antibody. The 
number of negative
slides we run depends on the pre-treatment of the antibodies requested on 
the case. We run one
negative slide for each method i.e. no pre-treatment, protease and pressure 
cook. Are we doing
more negative control slides than what is required ? I know some people just 
do one negative
slide per case. Any comments on the proper way of running negative immuno 
controls? Thanks
in advance for your inputs !


Anissa Choi
Burnaby Hospital
Burnaby, B.C.
Canada

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