[Histonet] S-100 background staining in rat tissue

[Sharon E Willman]

Hi,
We are currently developing S-100 immunostain in rat tissue on
the Ventanna.  We have used several protocols, but seems to have
a lot of background staining.  Does anyone have any suggestions
as to how to decrease the background?  We have decreased the time
for the primary, added AB blocker to the protocol, but still have
a great deal of background staining.

Any help would be most appreciated.  Thank you in advance!

Sharon Willman