[Histonet] cutting rat brains

Tue May 4 10:11:12 CDT 2004

Hi David,

Creases come from waterbath temp being too low. Tissue separation can be
caused be water temp being too high and/or you have left the block on water
too long.

Cheers

Steve

                      "David Clark"                                                                                                                  
                      <clarkda <@t> ohsu.edu>                     To:      Histonet <@t> lists.utsouthwestern.edu                                              
                      Sent by:                               cc:                                                                                     
                      histonet-bounces <@t> lists.utsouth         Subject: [Histonet] cutting rat brains                                                  
                      western.edu                                                                                                                    

                      03/05/2004 14:39                                                                                                               

Hello,

I was wandering if any of you wonderful Histonet people who have much
experience cutting rat brains can offer some assistance in trying to cut
good quality sections. We either encounter lots of wrinkles or tissue
seperation from the paraffin sections once placed on the waterbath. Is
this a processing problem or do you think it is strictly a sectioning
problem?

Any suggestions would be appreciated.

Thanks,

David Clark
Neuropathology
OHSU

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