Fwd: Re: [Histonet] Decalcifying mouse heads
Caroline Stott
caroline.stott <@t> anatomy.otago.ac.nz
Wed Mar 31 15:52:23 CST 2004
We use 10% formic acid. We use the chemical test to check if
decalcification is complete. That is 5ml of ammonium hydroxide added to
5ml of the bone solution and add 5ml saturated ammonium oxalate. If the
solution is milky white, there is still calcium present. So keep changing
the solution every second day until it is finished.
Caroline
Caroline Stott
Histology Service Unit
Medical School
University of Otago
Dunedin
(03) 479 7152
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