[Histonet] Mouse pancreas

[Jackie.O'Connor <@t> abbott.com]

The processing schedule you are using would make fibroid uterus brittle. 
What is prosoft?  Anyway - 30 minutes in two changes each of (ethanol) 
70%, 95%, Absolute, Xylene, and 2 changes of paraffin at 55 minutes each 
should do you just fine - for just pancreas - since it is so delicate.  I 
use the above schedule (45 minutes each station) for routine mice tissue 
and xenografts with beautiful results.

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotherapeutics





"Grant, Debra" <DRG <@t> Stowers-Institute.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/31/2004 01:51 PM

 
        To:     "Histonet" <histonet <@t> pathology.swmed.edu>
        cc: 
        Subject:        [Histonet] Mouse pancreas


Hi Histonetters,

I am looking for a good tissue processing schedule for mouse pancreas. I
am currently using the following schedule and having trouble with
sectioning the pancreas, the H&E's look cracked and parched as if my
water bath is too hot. The temperature on my water bath is 42-43 degrees
Celsius, I have a thermometer also that I check the temp with. I use
Tissue Prep paraffin for infiltration and embedding. 

Fixed by the researcher in 4% PFA 24 hours rinsed in distilled water 5'
x 3. 

70% Alcohol- 30 min.
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour 30 min.
Clearite 3- 1 hour 15 min.
Clearite 3- 1 hour 15 min.
Clearite 3- 1 hour 15 min.
Paraffin- 1 hour
Paraffin- 1 hour
Paraffin- 1 hour
Paraffin- 1 hour


Thanks in advance!


Debby Grant
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg <@t> stowers-institute.org
 

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