Fwd: Re: [Histonet] Decalcifying mouse heads

[Atoska S. Gentry]

please what percent formic acid?

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>From: Caroline Stott <caroline.stott <@t> anatomy.otago.ac.nz>
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>Subject: Re: [Histonet] Decalcifying mouse heads
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>Hi there,
>We used formic acid to decalcify our mouse heads.  They didn't take too 
>long (1 week) although I cant remember how long precisely.  And yes they 
>were used for immuno studies.  We had a few studies, one for temporal 
>bones and another for the whole head.  They worked fine.
>Caroline
>
>
>We are interested in decalcifying whole heads from adult mice in order to 
>examine histological sections throughout the oral cavity and esophagus. 
>Are you aware what would be appropriate for such a large piece of tissue, 
>or how long it would take to decalcify? In the future we would like to 
>have the option of performing immunohistochemistry on our decalcified 
>samples. Do you have any experience with IHC on such large decalcified samples?
>
>
>
>Caroline Stott
>
>Histology Service Unit
>Medical School
>University of Otago
>Dunedin
>(03) 479 7152
>
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Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850