[Histonet] Apoptosis detection -Caspase 3-
Serrano del Pozo, Erika
ESerrano <@t> imim.es
Wed Mar 31 02:09:38 CST 2004
Hi Jackie,
As Joelle, I am looking for an ICC technique to detect apoptosis cells,
instead mine are muscular cells (C2C12).
I am really interested in the Caspase 3 antibody. Could you tell me which
commercial antibody do you use and the protocol to perform the ICC ( I´m
used to perform IHC not ICC, and I´m not really sure if there is any "triky"
step).
Thanks in advance.
> Erika Serrano
>
> Centre de Regulació Genòmica
> Differenciation and Cancer Programme
> Barcelona -SPAIN-
>
>
> ----------
> From: Jackie.O'Connor <@t> abbott.com
> Sent: Tuesday, March 30, 2004 15:06 PM
> To: Joelle Alcock
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Apoptosis detection
>
> The Caspase 3 antibody demonstrates only apoptotic cells.
>
> Jackie O'Connor
> Abbott Laboratories
> Abbott Park, IL
>
>
>
>
>
>
>
>
> "Joelle Alcock" <plxja <@t> nottingham.ac.uk>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 03/29/2004 03:51 PM
>
>
> To: <histonet <@t> lists.utsouthwestern.edu>
> cc:
> Subject: [Histonet] Apoptosis detection
>
>
> Hi all
>
> I am looking for an IHC technique to detect apoptosis cells on testis
> sections. I have previously used the Tunnel assay but would like an
> alternative as often meiotic cells are also labelled and I want to avoid
> this.
>
> I have seen the Histone labelling assay by UPSTATE but I think that is
> mainly for ICC not IHC.
>
> Thanks for any help
>
> Joelle Alcock
>
> Institute of Genetics
> Queens Medical Centre
> Nottingham, UK
>
>
>
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