[Histonet] Fixation for lectin work

Robert Schoonhoven rschoon <@t> email.unc.edu
Tue Mar 30 13:19:55 CST 2004


Must be more to it then that.  Bouin's is made up with formaldehyde.

Robert Schoonhoven

Pablo Sánchez Quinteiro wrote:

>I do not know the reason, but I remember that few years ago that fact
>caused me chronic headache. Just after switching to Acetic-Alcohol fixed
>material I got nice labelling. Bouin fluid is also an excellent fixative
>for lectin studies.
>
>Sometimes aldehydes work in some tissues, so before working with lectins it
>is advisable to compare different fixatives.
>
>Cheers
>
>Pablo Sanchez
>
>At 12:04 p.m. 30/03/04 -0600, you wrote:
>  
>
>>Dear Histonetters:
>>
>>Does anyone know why 10% buffered formaldehyde may not be suitable for 
>>lectin labeling?
>>
>>John C. Dennis
>>
>>
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>>
>
>
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