[Histonet] About H2O2 and CD Markers

[Luis Chiriboga]

I have used up to 10% H2O2(5 minutes Max) for extremely bloody cell blocks
(FFPE) but wasn't looking at CD markers. Routinely use ~3% for B&T cell
markers on FFPE and haven't noticed any deleterious effects.  I have also
used for (<3%diluted in PBS, no methanol) for frozen sections CD staining.
Just need to be careful, bubbling doesn't lift off section. You will need to
determine time and concentration to best suit your tissue and needs.
Hope this helps
Luis

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Jorge
Ivan Zapata Valencia
Sent: Friday, March 26, 2004 7:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] About H2O2 and CD Markers


Hello everybody.
I have a simple question: do someone know the effect, if any, of the
different concentrations, and times, of H2O2 on the CD markers (Paraffin
and/or frozen sections)? I have read that some people use 0.01%, others 0.1%
and others 3% for different times, usually 10 or 30 minutes. What could
happen if I use a longer time?
Thank You very much.

JORGE IVAN ZAPATA

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