[Histonet] CD31/PECAM-1
Gayle Callis
gcallis <@t> montana.edu
Mon Mar 29 09:58:23 CST 2004
Isaiah,
Another possibility is make the antibody from cell line clone (ATCC). The
supernate can be used for staining, undiluted, and can contain around 10
ug/ml of antibody. We do this frequently with antibodies we use in large
quantities.
At 07:41 PM 3/27/2004 -0600, you wrote:
>Histonetters,
>
>Our lab has used Pharmingen's CD31 antibody (cat# 01951A/D) with great
>results for a couple years for paraffin-embedded mouse tissue IHC.
>Recently, they decided to "sublet" the production of this antibody to a
>company called Cymbus, which sells the antibody at 1/10th the original
>protein concentration. We've called Pharmingen regarding this, and they
>staunchly maintain that there is no difference between their antibody
>and the Cymbus rendition.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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