[Histonet] Rossman's fluid

Geoff McAuliffe mcauliff <@t> umdnj.edu
Wed Mar 24 15:12:20 CST 2004


Hi Christine:

    Picric acid is only dangerous if allowed to dry out and then 
subjected to severe shock or high temperatures (well above the boiling 
point of water). Much of the picric acid paranoia is spread by 
unsubstantiated rumors. Still, if you don't want it in your lab, try a 
formalin+alcohol+acetic acid mixture or Bouin's (I think you can buy 
Bouin's made up or sat. aqueous picric acid, which minimizes the risks). 
You could also ask your local lab safety police to store a small 
quantity of picric acid for you.
   
Geoff

Christine Mather wrote:

>I would like to try an old method for fixing early chicken embryos that uses
>Rossman's fluid. It's not commercially available and uses picric acid,
>rather nasty stuff. Are there any modern alternatives that will do the same
>job?
>
>Thanks,
>
>Christine.
>
>
>
>
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-- 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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