[Histonet] CD4/CD25/TGFB Antibodies; Filter choices for Leica

[Gayle Callis]

What species are you working with?  Mouse?  Human?  

At 09:36 AM 3/24/2004 +0000, you wrote:
>Dear All,
>
>As part of a study on mucosal immunity in The Gambia, W. Africa, I plan to
double stain antral and duodenal specimens for CD4 and CD25 & CD8 and CD25.
 Also, we would attempt to triple stain for TGF beta on these subsets.  We
have access to a Leica DMRXE although the filter cubes are all currently
long pass suppression filters . I have both formalin-fixed and frozen
sections of the antrum and the duodenum.  
>
>I would like to ask for advise on several points:
>
>1.	Can anyone share their experience on antibody combinations known to
work for CD4-25, CD8-25 and triple staining for CD4-CD25-TGFB either with
vectastain elite (peroxidase) or immunofluorescence.  I have compiled a
list of CD4, CD8, CD25, TGFB antibodies from Dako, Ancell, Novocastra, BD,
and Serotec, but before purchasing any of these, I thought it would be wise
to ask advise from the Histonet community.
>
>2.	I would probably need to acquire different filter cubes to do
simultaneous imaging of fluorescence.  There are several suppliers I have
encountered on the net (Omega Filters and Chroma Technology) * does anyone
have experience on using filters from these companies on Leica microscopes
(specifically the DMRXE).  Any problems encountered with using third-party
filters with Leica microscopes?
>
>Thanks.
>Juan
>
>
>Dr. Juan Antonio Solon
>MRC Keneba Field Station
>MRC Laboratories Gambia
>Atlantic Road, Fajara
>PO Box 273, Banjul
>The Gambia
>Email :  juan.solon <@t> lshtm.ac.uk
>Tel No:  (++220) 541021
>Fax No: (++220) 541022 / (++220) 496513
>
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>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)