[Histonet] blood vessel staining?

[rocan <@t> mac.com]

Sylvia,
The Pharmigen antibody works great but you may have to do some antigen 
exposure. We have found that the best staining is on thick frozen 
sections(20um) and we leave a 1:100 dilution incubating overnight AT 
ROOM TEMPERATURE!
Our secondary is an anti-rat biotinylated (I think this one is from 
Biomeda).  We then use the ABC kit and DAB from vector.
Good Luck
Rocio

On Mar 23, 2004, at 2:46 PM, Sylvia Poulos wrote:

> Hi all,
> I've got some formalin fixed paraffin embedded mouse tissue.  Any idea
> of something I could do to stain capillaries in these?  Someone here
> mentioned mason's trichrome.  Any thoughts are always welcome. Thanks!
> Sylvia
>
> Sylvia P. Poulos
> USDA-ARS-Animal Physiology Research Unit
> Athens, GA 30605
> 706-583-8279
> 706-542-0399 (fax)
>
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