[Histonet] cryo preserving and sectioning of bone

Gareth Davis mrsgbd2001 <@t> yahoo.com
Tue Mar 23 15:20:51 CST 2004


Hello again,
Does anyone have a good protocol for preparing bone (after decal is done) for cryosectioning.  I've done the sucrose preservation and also have just gone straight to OCT and immediate freezing with LN2.  Both protocols have worked for me.  But, I was just wondering what the consensus was.
Thanks,
Ms. Gareth Davis
Research Assistant
Tennessee State University

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