[Histonet] IHC on blood and cytology smears (ICC)

[Jan Shivers]

Could someone please send me a protocol for performing IHC/ICC on smears (I
normally only do FFPE slides)?

I'd like info mostly on if the slides have to be coated/charged , smears
air-dried or fixed (and in what) for how long, and if you do any
modification of the immuno protocol, such as blocking endogenous peroxidase
later in the procedure (and with what concentration of H2O2), and/or
changing incubation times/dilutions.

I've done smears on very rare occasions, but have had trouble getting the
cells to remain on the uncoated slides that the smears are being made on.

Thank you very much in advance.

Jan Shivers
Univ of Minnesota Vet Diag Lab
shive003 <@t> tc.umn.edu