[Histonet] Mouse Lung

[Starkus, Laurie]

I've recently started working with mouse lung and was given a protocol for
processing.  The protocol calls for 0.85% NaCl for 60 minutes at 4 degrees
celsius.  Then a 1:1 0.85% NaCl to Absolute ethanol for 30 minutes at room
temp.  Then the processing looks rather normal.  Since my experience is with
human tissue, I've never seen a sodium chloride step in processing.  What
does it do?  And, is it really necessary?  This is mouse lung infected with
TB or cryptococcus.

Thanks in advance.