[Histonet] CD marker in zinc fixed mouse liver
Graves, Kerry
kegraves <@t> utmb.edu
Sun Mar 21 20:03:02 CST 2004
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Jorge Ivan Zapata Valencia
Sent: Sun 3/21/2004 6:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc:
Subject: [Histonet] CD marker in zinc fixed mouse liver
hello everybody.
I am new in immunohisotology and I have trying to stain for CD4,CD8 and
CD45R but i had problems to get good staining. I am using Pharmingen
protocol (rat primary antibody, biotinylated goat anti rat secondary
antibody, streptavidina-HRP). I have tried H2O2 as endogenous peroxidase
blocking solution (3% in Metanol) but eritrocytes are still brown after DAB.
I am blocking with white egg and skim mil, for endogenous biotin.
May one help me? May I have any good protocol?
Thank you very much.
JORGE IVAN ZAPATA
Universidad del Valle
Cali, Colombia
_________________________________________________________________
Protect your PC - get McAfee.com VirusScan Online
http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
More information about the Histonet
mailing list