[Histonet] CD marker in zinc fixed mouse liver

[Jorge Ivan Zapata Valencia]

hello everybody.
I am new in immunohisotology and I have trying to stain for CD4,CD8 and 
CD45R but i had problems to get good staining. I am using Pharmingen 
protocol (rat primary antibody, biotinylated goat anti rat secondary 
antibody, streptavidina-HRP). I have tried H2O2 as endogenous peroxidase 
blocking solution (3% in Metanol) but eritrocytes are still brown after DAB. 
I am blocking with white egg and skim mil, for endogenous biotin.
May one help me? May I have any good protocol?
Thank you very much.

JORGE IVAN ZAPATA
Universidad del Valle
Cali, Colombia

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