[Histonet] help with elastic staining

[Gudrun Lang]

As far as I know, Weigert stains the nuclei. So if your nuclei are
pale-black Weigert has worked.
Elastic fibers are stained by Resorcin Fuchsin. In our procedure there is a
"washing-out-step" afterwards. Perhaps it was too long?
There is a good description on the following website:
http://www-medlib.med.utah.edu/WebPath/HISTHTML/MANUALS/WEIGERTS.PDF

greetings
Gudrun Lang

----- Original Message -----
From: "Deb Stults" <DSTULTS <@t> fremontmemorial.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, March 19, 2004 7:48 PM
Subject: [Histonet] help with elastic staining


   We ran into a problem today with an elastic stain on an artery biopsy.
We're pretty sure it is due to a reagent problem, but not sure which one.
The elastic fibers did not stain at all.  We use Weigert's iron hematoxylin
solutions A and B, which are new bottles and worked yesterday in another
stain (we make up fresh before each use),  the Resorcin Fuchsin working
solution expired last week, and our new bottle (previously ordered) has not
yet arrived, and the Van Gieson's solution is brand new just opened today.
Since the fibers are not black, that makes me think that its the Weigert's,
but since the Resorcin Fuchsin is expired, we weren't sure if it could be
the problem.  If anyone has any suggestions, please let me know asap.  The
pathologist doesn't want to wait for new reagent to come in.
                                           Thanks,
                                                  Deb and Karen


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