[Histonet] help with elastic staining

Drew Allan Roenneburg ROENN <@t> surgery.wisc.edu
Fri Mar 19 13:33:07 CST 2004


Deb and Karen
Do you make up any of your own solutions?  Try combining 30 mL of a 5%
alcoholic hematoxylin, 10mL of a 10% aq. ferric chloride, and 10 mL
Verhoeff's iodine soln (Potassium iodide and Iodine).  Stain for 12
mins.  Wash under running tap water.  Differentiate by dipping slide in 
2% aq ferric chloride, checking microscopically between.  
Drew




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