[Histonet] active-Caspase
Patsy Ruegg
pruegg <@t> colobio.com
Fri Mar 19 13:09:55 CST 2004
if you are using a biotinylated antibody and/or detection system your
non-specific staining is probably due to endogenous biotin in liver and in
my experience even with the most aggressive ab block you cannot get rid of
the biotin in liver.
Patsy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Dixon,
Leslie E.
Sent: Thursday, March 18, 2004 8:59 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] active-Caspase
Hello all,
We are having issues with non-specific background staining. I am interested
in anyone's protocol for anti-active Caspase-3 on mouse livers. Any help
would be greatly appreciated.
Thanks,
Leslie
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