[Histonet] digesting soft tissue

[Philip Oshel]

Karl,

NaOH or KOH or bleach, I've used all. Check the fish people on campus 
-- this is a routine matter for them.
Mind, using base or bleach creates a pleasant odor your lab mates 
will be hanging you for.

Phil

>Greetings,
>I'm trying to find a good cocktail for non-specific digestion of 
>tissue from bone samples--only the mineralized bone needs to be left 
>unscathed.  I'm looking for a relatively cheap solution that will 
>yield nice clean bones.  I'm aware of a product from Fisher, but it 
>costs about $70/100ml. It seems to me that there must be a simple 
>solution that I'm just not aware of (aside from culturing maggots). 
>Thanks in advance for any suggestions.
>Regards,
>Karl G.
>
>--
>Karl Garsha
>Light Microscopy Specialist
>Imaging Technology Group
>Beckman Institute for Advanced Science and Technology
>University of Illinois at Urbana-Champaign
>405 North Mathews Avenue
>Urbana, IL 61801
>Office: B650J
>Phone: 217.244.6292
>Fax: 217.244.6219
>Mobile: 217.390.1874
>www.itg.uiuc.edu
>
>
>
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-- 
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison,  WI  53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)