[Histonet] digesting soft tissue
Geoff McAuliffe
mcauliff <@t> umdnj.edu
Thu Mar 18 18:10:24 CST 2004
Household bleach, 1 part bleach to 4 parts water.
or
Adolph's meat tenderizer is papain, don't know the concentration needed.
You can get papain form Sigma, works well at 37C. I don't know any other
details.
Karl Garsha wrote:
> Greetings,
> I'm trying to find a good cocktail for non-specific digestion of
> tissue from bone samples--only the mineralized bone needs to be left
> unscathed. I'm looking for a relatively cheap solution that will
> yield nice clean bones. I'm aware of a product from Fisher, but it
> costs about $70/100ml. It seems to me that there must be a simple
> solution that I'm just not aware of (aside from culturing maggots).
> Thanks in advance for any suggestions.
> Regards,
> Karl G.
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
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