[Histonet] mouse bone osteoblast IHC

Jan Shivers shive003 <@t> umn.edu
Thu Mar 18 13:50:55 CST 2004


I am posting this for an MD not on the Histonet listserv; he is requesting
information on staining mouse osteoblasts using the IHC method.  (I have had
no experience with osteoblasts myself).

Does anyone know of an antibody source for detecting murine osteoblasts (or
one made against another species that will cross-react)?

What decalcification method is used on the bone samples?

Any complete protocols that anyone is willing to share would be greatly
appreciated.

Thanks...

Jan Shivers
U of MN Vet Diag Lab





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