[Histonet] iron stain on bone marrow

[Patsy Ruegg]

Actually we used to get our best iron stains on smears since processing can
leach out iron in the tissue.  Perhaps a shorter fixation in methanol would
help, alcohol can also leach out the hemosiderin.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Wendy
England
Sent: Wednesday, March 17, 2004 1:28 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] iron stain on bone marrow


Thanks again to everybody who gave me the information about this stain!
Here comes another question: I couldn't see much iron on my slide, only
about 2~3 spots per rat bone marrow slide. I fixed them in Methanol for 15
min, then in freshly mixed stain solution(equal part of 20% HCl and 10%
potassium ferrocyanide) for 30 min in 50-60C. Then counterstain in Safranin
after wash. Then wash, air dry then mount.

I was told there should be much more iron. I feel bad now. Could any body
give me advice or could I ask if it's possible due to the bone marrow
preparation  since we indeed saw some spots. I didn't prepare the sample.

Thanks and look forward to hear from you there!

Wendy

_________________________________________________________________
FREE pop-up blocking with the new MSN Toolbar – get it now!
http://clk.atdmt.com/AVE/go/onm00200415ave/direct/01/


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet