[Histonet] iron stain on bone marrow-SOLUTIONS
Dunikoski, Leonard PhD
ldunikoski <@t> rbmc.org
Thu Mar 18 12:35:06 CST 2004
"Remember the order: acid to water"
-----Original Message-----
From: Bonner, Janet [mailto:Janet.Bonner <@t> FLHOSP.ORG]
Sent: Thursday, March 18, 2004 12:56 PM
To: 'Wendy England'; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] iron stain on bone marrow-SOLUTIONS
The 20% Hydrochloric acid solution should be added to the 10% Potassium
ferrocyanide solution - (not 10% potassium ferrocyanide to 20% Hydrochloric
acid solution) when making the 1:1 mixture.
-----Original Message-----
From: Wendy England [mailto:weneng <@t> hotmail.com]
Sent: Wednesday, March 17, 2004 3:28 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] iron stain on bone marrow
Thanks again to everybody who gave me the information about this stain! Here
comes another question: I couldn't see much iron on my slide, only
about 2~3 spots per rat bone marrow slide. I fixed them in Methanol for 15
min, then in freshly mixed stain solution(equal part of 20% HCl and 10%
potassium ferrocyanide) for 30 min in 50-60C. Then counterstain in Safranin
after wash. Then wash, air dry then mount.
I was told there should be much more iron. I feel bad now. Could any body
give me advice or could I ask if it's possible due to the bone marrow
preparation since we indeed saw some spots. I didn't prepare the sample.
Thanks and look forward to hear from you there!
Wendy
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