[Histonet] Please Help with Cresyl Violet Acetate
Featherstone, Annette
AFeatherstone <@t> KaleidaHealth.Org
Thu Mar 18 12:01:52 CST 2004
Our Cresyl Echt Violet is
CEV Stock Sol. CI-51010 --5gm
D. H2O----80 ml
Absolute--20 ml
Warm the distilled, add the CEV mix, then add alcohol.
Working CEV solution
45 ml of stock
Acetic Acid, glacial--15 drops
Deparaffinize, stain in working sol for 8 minutes, dehydrate, clear.
Nissl substance and nuclei----Blue-purple
Background--------------------Colorless
Annette Featherstone HT/MLT
-----Original Message-----
From: Sebree Linda A. [mailto:la.sebree <@t> hosp.wisc.edu]
Sent: Thursday, March 18, 2004 11:43
To: Histonet (E-mail)
Subject: [Histonet] Please Help with Cresyl Violet Acetate
Good Morning Histoneters,
I am trying to work-up a CEVstain for nissel substance and I am having
difficulties producing a magenta effect on the nissel. I have tried several
powders with the CI # of 10510-54-0 along with different staining
procedures. I would appreciate any help or suggestions anyone has to offer.
Angela Baker
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
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