[Histonet] ENaC-alpha Antibody

[Christl]

Hi all Histonetters

I would like to know if there is anyone out there who has used the
polyclonal Anti-Rat ENaC-alpha antibody from Alpha Diagnostics in the
UK? We are trying to get it to work but there seem to be no literature
on this. We are using normal rat kidney as a positive control.

I have to confess, we've only tried it at three different dilutions and
three incubation times with pressure cooking as the Ag retrieval method
of choice at the moment. However, seeing as we have very little
antibody, I don't want to let ANY go to waste unnecessarily!

Any suggestions would be helpful!

Thanks 
Christl