[Histonet] staining frozen sections

[jason madore]

We are having problems with staining our frozen sections. Slides which once 
gave excellent morphology soon begin to loose their nuclei. The nucleus 
looks empty or washed away leaving white holes where the nucleus was. We are 
cutting the slides at -20. Storing at -80 wrapped in tin foil with 
dessicant. Warm to RT for 30seconds and fix in cold acetone\EtOH for 5 
minutes, wash in dH2O for 1 minute,  and then stain with hematoxilyn, 
dehydrate, clear in xylenes, then mount. Does anyone know what could be 
causing this?
Thanks. for any help.
Jason.

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