[Histonet] smooth muscle alpha actin antibody for mouse cells andmouse tissue?

[Gayle Callis]

With mouse on mouse IHC staining, have you tried the DAKO ARK kit?  We also
had less background using the Chemicon Mouse on Mouse kit, they will supply
you with a free sample kit, so be sure to ask for one.  Nice when a company
does that for us. 

At 10:20 AM 3/15/2004 -0500, you wrote:
>Hi everyone - I want to stain mouse tissues and smooth muscle cells in 
>culture for alpha-actin.  Apparently clone 1A4 (mouse monoclonal) is 
>quite popular, but my PI wants me to find an antibody from a different 
>species (rabbit, for example) to prevent excessive background staining.  
>Any recommendations?  And what dilutions do you use? Thank you!! - Jen

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)