Réf. : [Histonet] organotypic cultures

Mon Mar 15 06:32:34 CST 2004

Don't understand why you want to perfuse and after do organo culture. Never
heard of this!

 We dissect as quick as possible, use a tissue chopper to get those 400µ
slice, get them off the chopper support with flush of culture medium into a
25ml Corning tube. Swirl them around a little because this helps separate
slices that stick together. Then under a disecting microscope, pick out
slices that seem nicest then scoop them up with the flat side of two
"paddle pastette" (Alpha laboratories cat n° LW4295 address 40 Parham
drive, Eastleigh, Hampshire SO50 4NU UK tel. 44-1703483000). We sterilize
the paddles  in ETOH.
Make sure you preincubate the millicell inserts with culture medium a
couple of hours before use. This seems to increase health and survival.

good luck,
Nancy Walker
Molecular Pharmacology

Sanofi-Synthelbo Research
B.P. 37 Labége Innopole
31676 LABEGE CEDEX FRANCE

nancy.walker <@t> sanofi-synthelabo.com
tel : (33)561004179  fax :(33)561004001